CN107043097B - By the method and its carbon dots of mushroom preparation carbon dots and application - Google Patents
By the method and its carbon dots of mushroom preparation carbon dots and application Download PDFInfo
- Publication number
- CN107043097B CN107043097B CN201710042843.1A CN201710042843A CN107043097B CN 107043097 B CN107043097 B CN 107043097B CN 201710042843 A CN201710042843 A CN 201710042843A CN 107043097 B CN107043097 B CN 107043097B
- Authority
- CN
- China
- Prior art keywords
- carbon dots
- mushroom
- carbon
- powder
- dots
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2002/00—Crystal-structural characteristics
- C01P2002/70—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2002/00—Crystal-structural characteristics
- C01P2002/80—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70
- C01P2002/82—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70 by IR- or Raman-data
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/01—Particle morphology depicted by an image
- C01P2004/04—Particle morphology depicted by an image obtained by TEM, STEM, STM or AFM
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Materials Engineering (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Carbon And Carbon Compounds (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
It is 5-7 hours obtained carbon dots of 180-200 DEG C of reaction after mixing mushroom powder and water in the ratio of 0.08g ~ 0.1g:1mL the invention discloses a kind of method by mushroom preparation carbon dots.The protophyte resource mushroom that the present invention is widely present using nature is cooked carbon source, carbon dots are prepared by simple hydro-thermal method, it is environmentally protective, gained carbon dots have good fluorescence property, also have many advantages, such as that good light stability, toxicity be low, good biocompatibility, in terms of have a good application prospect.
Description
Technical field
The invention belongs to the preparation technical fields of luminescent material, are related to a kind of method by mushroom preparation carbon dots, further relate to
The application of carbon dots and the carbon dots obtained by this method.
Background technique
In recent years, it is received more and more attention as an emerging important member, carbon dots in carbon nanomaterial family.
In addition to have similar to traditional semiconductor-quantum-point excellent optical property, carbon dots also have good light stability, toxicity it is low,
Traditional quantum dot such as good biocompatibility and environment friendly, preparation condition are mild, step is simple, abundant raw material is cheap without
Analogous advantage.Currently, the research emphasis of carbon dots concentrates on finding easy preparation method and answering in fluorescence analysis
With.
The synthesis of carbon dots is generally divided into method (such as arc discharge method, laser ablation, electrochemical synthesis and acid from top to bottom
Oxidizing process etc.) and method (such as microwave method, hydro-thermal method and ultrasonic method) two major classes from bottom to top.From top to bottom method by physics or
From large to small by the carbon material of bulk, size is until nanoscale for the method for chemistry.Method is that have with the carbon containing of small size from bottom to top
Machine object is carbon source, is changed from small to big by orderly or unordered being assembled of small molecule, to reach certain size to prepare
Carbon dots.The carbon dots that these two kinds of methods prepare all show the excellent characteristics of luminescence.It is basic to prepare carbon source used in carbon dots
On can be divided into inorganic carbon source and organic carbon source two major classes.Inorganic carbon source mainly has candle ash, graphite powder, active carbon, carbon nanometer
Pipe and carbon fiber etc..It is main using method from top to bottom when preparing carbon dots with inorganic carbon source.Organic carbon source has glucose, amino
The environmentally protective nature such as the carbon containing organic chemical reagent such as acid and grass, pericarp, sweet potato, coffee grounds, capsicum, apple and sugarcane
Product.Method from bottom to top is mainly used when preparing carbon dots with organic carbon source, most common method has hydro-thermal method and microwave method.But
So far, there is not yet being reported in the method that mushroom class prepares carbon dots as carbon source.
Summary of the invention
In view of this, it is an object of the invention to the protophyte resource mushrooms being widely present using nature to do carbon source,
Carbon dots are prepared by simple hydro-thermal method, it is environmentally protective.
Through studying, the invention provides the following technical scheme:
1. by the method for mushroom preparation carbon dots, which is characterized in that by mushroom powder and water in the ratio of 0.08g ~ 0.1g:1mL
After mixing, 5-7 hours obtained carbon dots of 180-200 DEG C of reaction.
It is furthermore preferred that after mushroom powder and water are mixed in the ratio of 0.1g:1mL, 200 DEG C of reactions, 6 hours obtained carbon dots.
Preferably, reaction solution is cooling, supernatant is collected in centrifugation, the filtering with microporous membrane for being 0.22 μm with aperture, filtrate
It is dry after being dialysed with the bag filter that molecular cut off is 100, obtain carbon dots powder.
It is furthermore preferred that reaction solution is cooling, 10000rpm is centrifuged 15 minutes, collects supernatant, is 0.22 μm with aperture
Filtering with microporous membrane, after the bag filter that filtrate molecular cut off is 100 is dialysed 24 hours, freeze-drying obtains carbon dots powder.
Preferably, the mushroom powder is by new fresh mushroom in 60 DEG C ~ 80 DEG C after drying 24 ~ 48 hours, with mortar grinder at
Powder.
Using carbon dots made from the above method.
Application of the carbon dots in terms of cell imaging.
The beneficial effects of the present invention are: the present invention provides a kind of methods by mushroom preparation carbon dots, utilize nature
The protophyte resource mushroom being widely present is cooked carbon source, prepares carbon dots by simple hydro-thermal method, environmentally protective, gained carbon dots tool
Have good fluorescence property, also have many advantages, such as that good light stability, toxicity be low, good biocompatibility, in terms of
It has a good application prospect.
Detailed description of the invention
Fig. 1 is prepared the method schematic diagram of carbon dots by mushroom.
The characterization of Fig. 2 carbon dots: the fluorescence excitation and emission spectra of (A) carbon dots, wherein illustration is carbon dots dispersion liquid visible
Photo under light (I) and ultraviolet light (II)；(B) the high resolution TEM figure of carbon dots；(C) infrared spectroscopy of carbon dots；(D) carbon
The X-ray energy spectrum of point.
Emission spectrum of Fig. 3 carbon dots under different excitation wavelengths.
The study on the stability of Fig. 4 carbon dots: (A) saline-alkaline tolerance is investigated；(B) study on the stability under different pH condition；(C)
Stablize the time to investigate；(D) study on the stability in different organic reagents.
Toxicity test of the carbon dots of Fig. 5 various concentration to CKK-8 cell.
Fig. 6 is prepared the condition optimizing of carbon dots by mushroom: (A) reaction time optimizes；(B) reaction temperature optimizes.
NaCl(under Fig. 7 (A) ultraviolet lamp is left) and carbon dots (right side)；(B) NaCl(under fluorescent lamp is left) and carbon dots (right side).
The absorbent cotton (left side) of carbon dots dispersion liquid is not smeared under Fig. 8 (A) ultraviolet lamp and smears the absorbent cotton of carbon dots dispersion liquid
(right side)；(B) filter paper (left side) of carbon dots dispersion liquid is not smeared under ultraviolet lamp and smears the filter paper (right side) of carbon dots dispersion liquid.
Fluorescent image of Fig. 9 carbon dots in human A549 cell lines: (A) is cell photograph via bright field；(B) it is marked for carbon dots
(excitation wavelength 488nm) is imaged in cell fluorescence；(C) photo is superimposed for light field and fluorescence picture.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with attached drawing to of the invention excellent
Embodiment is selected to be described in detail.
Main agents used in preferred embodiment are as follows: mushroom is purchased from It In Beibei, Chongqing Yong Hui supermarket；Methanol
(Methanol), ethyl alcohol (Ethanol), acetone (Acetone), acetic acid (Acetic Acid), acetonitrile (Acetonitrile),
Dimethyl sulfoxide (DimethylSulfoxide), tetrahydrofuran (Tetrahydrofuran) and trichloroethanes
(Trichloroethane) it is purchased from Chengdu Ke Long chemical reagent factory；Disodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate
(NaH2PO4), glacial acetic acid (HAC), phosphoric acid (H3PO4), boric acid (H3BO3), sodium hydroxide (NaOH) and sodium chloride (NaCl) is purchased from
Beijing Ding Guo Biotechnology Co., Ltd；Experimental water is ultrapure water (Millipore, 18.25 M Ω).
Key instrument used in preferred embodiment is as follows: FA2004A electronic balance (the upper limited public affairs of the sub- instrument of Nereid's atmospheric electricity
Department), 25 ml polytetrafluoroethylene (PTFE) hydrothermal reaction kettles (Jinan China Heng Hua Science and Technology Ltd.), F-7000 type fluorescence spectrophotometry
It counts (Hitachi, Japan), Tecnai G2 F20 S-TWIN type transmission electron microscope (FEI Co., the U.S.), ESCALAB
250 model x-ray photoelectron spectroscopy (Thermo company, the U.S.), Prestige-21 Fourier infrared spectrograph (Japanese Shimadzu
Company), PiloFD8-4.3V type freeze drier (Xi Meng international corporation, the U.S.), DHG-9140A electric heating constant-temperature blowing drying box
(Shanghai Qi Xin scientific instrument Co., Ltd), the laboratory UPT Superpure water machine (the Chinese excellent general experimental instruments and equipment limited in Xi'an),
TG16-W high speed centrifugal machine for minim.
Embodiment 1 prepares carbon dots by carbon source of mushroom
1, the preparation of carbon dots
As shown in Figure 1, by new fresh mushroom after 80 DEG C 24 hours dry, with mortar grinder at powder；Take mushroom powder 0.6
6 mL of ultrapure water is added in g, mixes, sets in 25 mL polytetrafluoroethylene (PTFE) stainless steel cauldrons, and 200 DEG C are reacted 6 hours；Gained reaction
Liquid is cooled to room temperature, and 10000 rpm are centrifuged 15 minutes, collects supernatant, the filtering with microporous membrane for being 0.22 μm with aperture, filtrate
After being dialysed 24 hours with the bag filter that molecular cut off (MWCO) is 100, freeze-drying obtains carbon dots powder；Carbon dots powder is used
Ultrapure water dispersion obtains the carbon dots dispersion liquid of 1 μ g/mL, 4 DEG C of preservations.
2, the characterization of carbon dots
A length of 370 nm of the optimum excitation wave of carbon dots, best launch wavelength are 455 nm(Fig. 2A), be under visible light
Yellowish (the illustration I in Fig. 2A) issues hanced cyan fluorescent (the illustration II in Fig. 2A) under 365 nm ultraviolet lights.
Use the shape characteristic (Fig. 2 B) of high resolution TEM observation carbon dots, it is seen that carbon dots have good dispersion
Property, average diameter is 2.3 ± 1.3 nm.
Use the surface texture (Fig. 2 C) of FTIR spectrum analysis carbon dots, 3438 cm-1The peak at place is attributed to O-H and stretches
Contracting vibration, 1678 cm-1The peak at place is attributed to C=O stretching vibration, 2964 cm-1The peak at place is attributed to N-H stretching vibration, display
There are the characteristic groups such as carboxyl, hydroxyl, amino on the surface of carbon dots.
Surface composition and the elemental analysis (Fig. 2 D) of carbon dots are carried out using X-ray electron spectrum, it is seen that mainly deposit on spectrogram
At the peak (284.6,399.8 and 531.4 eV are respectively from C 1s, N 1s and O 1s) of C, N and O three types, illustrate carbon
Point doping N.
The wavelength of fluorescence of carbon dots has apparent Red Shift Phenomena with the increase of excitation wavelength, when excitation wavelength increases from 370 nm
450 nm are added to, the launch wavelength of carbon dots increases to 450 nm(Fig. 3 from 370 nm).
3, the study on the stability of carbon dots
(1) saline-alkaline tolerance is investigated: take carbon dots dispersion liquid, be added different final concentrations (0,0.1,0.2,0.3,0.4,0.5,
0.6,0.7,0.8,0.9,1.0 mol/L) sodium chloride, be incubated for, 455 nm of wavelength at measurement fluorescence intensity.As a result such as Fig. 4 A
Shown, the fluorescence intensity of carbon dots is not substantially change in the case that sodium chloride concentration is gradually increased in system, shows its tool
There is higher saline-alkaline tolerance.
(2) study on the stability under different pH condition: taking carbon dots dispersion liquid, different pH value (2,3,4,5,6,7,8,
9,10,11,12) under the conditions of be incubated for after, the measurement fluorescence intensity 455 nm of wavelength at, as a result as shown in Figure 4 B, the fluorescence of carbon dots
Intensity is slightly changed under different ph values, overall relatively stable, and the best pH value that is incubated for is 7.
(3) stablize the time to investigate: taking carbon dots dispersion liquid, be incubated for different time (1,2,3,4,5,6,7,8,9,10,11,12
Hour) after, fluorescence intensity is measured at 455 nm of wavelength.As a result as shown in Figure 4 C, the fluorescence intensity of carbon dots is steady in 12 hours
It is fixed, it does not change with time.
(4) study on the stability in different organic reagents: taking carbon dots dispersion liquid, different organic reagents (methanol, ethyl alcohol,
Acetone, acetic acid, acetonitrile, dimethyl sulfoxide, tetrahydrofuran, trichloroethanes) in be incubated for after, 455 nm of wavelength at measurement fluorescence it is strong
Degree.As a result as shown in Figure 4 D, carbon dots keep stablizing in most of organic reagents.
4, the Toxicity test of carbon dots
10 mg/mL carbon dots dispersion liquids of filtration sterilization are taken, being diluted to final concentration with fresh 1640 culture medium is respectively
2.5,2.0,1.5,1.0,0.5 mg/mL.Take the CKK-8 cell inoculation of logarithmic growth in 60 mm Tissue Culture Dish, totally 6 ware,
Being put into 24 h of cell incubator incubation makes cell enter logarithmic growth phase, absorbs culture solution in each culture dish, is separately added into aforementioned
3 mL of carbon dots dilution of various concentration, while solvent control group is set and (is added with physiological saline substitution carbon dots dispersion liquid
3 mL of 1640 culture medium), it is put into cell incubator culture 24 hours.As a result as shown in figure 5, even if at high dose (2.5mg/mL)
Under the conditions of being incubated for (24 hours) with long-time, carbon dots also hardly generate toxicity to cell, show good biocompatibility.
5, the fluorescence of carbon dots
The carbon dots powder for taking freeze-drying to obtain is compared under fluorescent lamp with NaCl in ultraviolet lamp respectively.As a result such as
Shown in Fig. 6, carbon dots powder shows apparent fluorescence (Fig. 6 A) in the UV lamp, and there is also apparent with NaCl under fluorescent light
Color distinction (Fig. 6 B).
Carbon dots dispersion liquid is applied on absorbent cotton and filter paper respectively, in the UV lamp with do not smear the de- of carbon dots dispersion liquid
Rouge cotton and filter paper are compared.As a result as shown in fig. 7, in the UV lamp, smear carbon dots dispersion liquid absorbent cotton and filter paper with not
The absorbent cotton and filter paper for smearing carbon dots dispersion liquid have apparent fluorescent differences.
Embodiment 2, the condition optimizing that carbon dots are prepared using mushroom as carbon source
1, the reaction time optimizes
By 1 the method for embodiment, 4,5,6,7,8,9 hours preparation carbon dots are reacted respectively in 200 DEG C.As a result such as Fig. 8 A institute
Showing, the fluorescent effect of carbon dots made from reaction 5-7 hours is preferable, and the reaction time most preferably 6 hours.
2, reaction temperature optimizes
By 1 the method for embodiment, respectively at 120,140,160,180,200 DEG C of reactions, 6 hours preparation carbon dots.As a result such as
Shown in Fig. 8 B, the 180-200 DEG C of fluorescent effect for reacting carbon dots obtained is preferable, and most preferably 200 DEG C of reaction temperature.
Embodiment 3, the application using mushroom as carbon dots made from carbon source in terms of cell imaging
Human lung adenocarcinoma cell (A549 cell) is inoculated in 6 holes in 96 microwell plates after confining liquid is added in every hole to be put into
In wet box, 37 DEG C, 5%CO are placed into2It is incubated for 30 minutes in incubator, takes out 96 microwell plates, it is first slow with the phosphoric acid of 0.01mol/L
Fliud flushing washes away confining liquid, then is blotted orifice plate surrounding liquid with filter paper, will be in carbon dots dispersion liquid made from embodiment 1 and 6 holes
A549 cell is in 37 DEG C, 5%CO2Incubator is incubated for 1 hour, is washed with the phosphate buffer of 0.01mol/L, and it is aobvious then to set fluorescence
The label situation of micro- microscopic observation cell.
Carbon dots mark A549 cell results as shown in Figure 9, it can be seen that A549 cell shows bright under fluorescence microscope
Aobvious green fluorescence illustrates that carbon dots produced by the present invention can be used as fluorescent dye for cell imaging.
Above-mentioned test result explanation, the present invention have good fluorescence property by carbon dots made from carbon source of mushroom, also have
Have the advantages that good light stability, toxicity be low, good biocompatibility, in terms of have a good application prospect.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although passing through ginseng
According to the preferred embodiment of the present invention, invention has been described, it should be appreciated by those of ordinary skill in the art that can
To make various changes to it in the form and details, without departing from the present invention defined by the appended claims
Spirit and scope.
Claims (5)
1. by the method for mushroom preparation carbon dots, which is characterized in that mix mushroom powder and water in the ratio of 0.08g ~ 0.1g:1mL
Afterwards, 5-7 hours obtained carbon dots of 180-200 DEG C of reaction, it is 0.22 μm with aperture that reaction solution is cooling, and supernatant is collected in centrifugation
Filtering with microporous membrane, it is dry after the bag filter dialysis that filtrate molecular cut off is 100, obtain carbon dots powder；The mushroom powder
It is in 60 DEG C ~ 80 DEG C after drying 24 ~ 48 hours, with mortar grinder at powder by new fresh mushroom.
2. as described in claim 1 by the method for mushroom preparation carbon dots, which is characterized in that mushroom powder and water are pressed 0.1g:1mL
Ratio mixing after, 200 DEG C of reactions, 6 hours obtained carbon dots.
3. as described in claim 1 by the method for mushroom preparation carbon dots, which is characterized in that reaction solution is cooling, 10000rpm
Supernatant is collected in centrifugation 15 minutes, is 0.22 μm of filtering with microporous membrane with aperture, filtrate molecular cut off be 100 it is saturating
After analysing bag dialysis 24 hours, freeze-drying obtains carbon dots powder.
4. using carbon dots made from any one of claims 1 to 3 the method.
5. application of the carbon dots described in claim 4 in terms of cell imaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710042843.1A CN107043097B (en) | 2017-01-20 | 2017-01-20 | By the method and its carbon dots of mushroom preparation carbon dots and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710042843.1A CN107043097B (en) | 2017-01-20 | 2017-01-20 | By the method and its carbon dots of mushroom preparation carbon dots and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107043097A CN107043097A (en) | 2017-08-15 |
| CN107043097B true CN107043097B (en) | 2019-04-19 |
Family
ID=59543968
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710042843.1A Expired - Fee Related CN107043097B (en) | 2017-01-20 | 2017-01-20 | By the method and its carbon dots of mushroom preparation carbon dots and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107043097B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108069414A (en) * | 2017-12-19 | 2018-05-25 | 重庆文理学院 | A kind of method for preparing carbon nanomaterial using discarded pine tree tower |
| CN108101022A (en) * | 2017-12-27 | 2018-06-01 | 重庆文理学院 | A kind of method that carbon quantum dot is prepared using fennel seeds as raw material |
| CN109593523A (en) * | 2018-11-13 | 2019-04-09 | 东北林业大学 | A kind of preparation method and application of hypha,hyphae nitrogen sulphur auto-dope carbon dots |
| CN109734073A (en) * | 2018-12-13 | 2019-05-10 | 南京师范大学 | It is a kind of using wormwood leaf as carbon quantum dot of carbon source and the preparation method and application thereof |
| CN109821525A (en) * | 2019-03-28 | 2019-05-31 | 东北林业大学 | A kind of preparation method loading biomass carbon point defect titanium dioxide optical catalyst |
| CN110194953A (en) * | 2019-06-27 | 2019-09-03 | 上海大学 | Biomass-based fluorescent carbon quantum dot, preparation method and application |
| CN110320196A (en) * | 2019-08-23 | 2019-10-11 | 云南健牛生物科技有限公司 | A kind of highly sensitive method for detecting inorganic mercury and organic mercury |
| TR201914839A1 (en) * | 2019-09-30 | 2021-04-21 | Selcuk Ueniversitesi | A method for obtaining fluorophore dyes from edible wild plants. |
| CN113789173B (en) * | 2021-09-16 | 2023-03-31 | 青岛农业大学 | Preparation method and application of edible fungus-based fluorescent carbon quantum dots for indicating freshness of food |
| CN114958359B (en) * | 2022-05-12 | 2023-10-13 | 青岛农业大学 | Simulated enzyme material copper-doped carbon quantum dot with mushrooms as carbon sources, and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103482609A (en) * | 2013-10-15 | 2014-01-01 | 浙江师范大学 | Preparation method of carbon quantum dots for detecting Fe<3+> in lake water |
| CN104192828A (en) * | 2014-08-26 | 2014-12-10 | 上海交通大学 | Hydrothermal synthesis method of carbon quantum dots from cotton |
| CN104531148A (en) * | 2014-12-31 | 2015-04-22 | 天津大学 | Preparation method of assembly of carbon quantum dots (CQDS) |
| CN104845618A (en) * | 2015-05-25 | 2015-08-19 | 福州大学 | Nitrogen-doped fluorescent carbon quantum dot and preparation method and application thereof |
| CN105462583A (en) * | 2015-11-03 | 2016-04-06 | 南京工业大学 | Preparation method and application of ethanol solution containing fluorescent nano-carbon quantum dots |
-
2017
- 2017-01-20 CN CN201710042843.1A patent/CN107043097B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103482609A (en) * | 2013-10-15 | 2014-01-01 | 浙江师范大学 | Preparation method of carbon quantum dots for detecting Fe<3+> in lake water |
| CN104192828A (en) * | 2014-08-26 | 2014-12-10 | 上海交通大学 | Hydrothermal synthesis method of carbon quantum dots from cotton |
| CN104531148A (en) * | 2014-12-31 | 2015-04-22 | 天津大学 | Preparation method of assembly of carbon quantum dots (CQDS) |
| CN104845618A (en) * | 2015-05-25 | 2015-08-19 | 福州大学 | Nitrogen-doped fluorescent carbon quantum dot and preparation method and application thereof |
| CN105462583A (en) * | 2015-11-03 | 2016-04-06 | 南京工业大学 | Preparation method and application of ethanol solution containing fluorescent nano-carbon quantum dots |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107043097A (en) | 2017-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107043097B (en) | By the method and its carbon dots of mushroom preparation carbon dots and application | |
| CN104789217B (en) | Amphipathy carbon quantum dot and preparation method thereof | |
| CN109179421B (en) | Method for preparing yellow or green silicon quantum dots | |
| Lin et al. | Eco-friendly synthesis of shrimp egg-derived carbon dots for fluorescent bioimaging | |
| CN105018082A (en) | Method for preparing carbon quantum dot labeled probe for silk fibroin extracted cell development | |
| CN106675557B (en) | Preparation method of a kind of N doping carbon quantum dot and products thereof, application | |
| CN108504349B (en) | Preparation method of rhodamine hybrid carbon dots and application of rhodamine hybrid carbon dots in mitochondrial targeting recognition | |
| CN109097036A (en) | A kind of longer emission wavelength fluorescent carbon quantum dot and preparation method thereof | |
| CN109321240B (en) | Orange fluorescent carbon dot and preparation method thereof | |
| CN106892421A (en) | A kind of red carbon quantum dot preparation method of full green | |
| CN106701069B (en) | A kind of preparation method of controllable, longwave transmissions the carbon-based nano dot of fluorescence of wavelength | |
| CN109734073A (en) | It is a kind of using wormwood leaf as carbon quantum dot of carbon source and the preparation method and application thereof | |
| CN110294471A (en) | A kind of synthetic method of the nitrogen co-doped graphene quantum dot of boron | |
| CN108084997A (en) | The preparation method of N doping fluorescent carbon quantum dots | |
| CN104479675A (en) | Large-scaled industrial preparation method for fluorescent graphene quantum dot material | |
| CN110257060A (en) | Utilize the method and product of resveratrol preparation carbon dots and application | |
| CN107603610A (en) | Preparation method from mature vinegar carbon nano-particles | |
| CN112126428A (en) | Orange fluorescent carbon dot and preparation method and application thereof | |
| Wan et al. | Regulation of multi-color fluorescence of carbonized polymer dots by multiple contributions of effective conjugate size, surface state, and molecular fluorescence | |
| CN107603612A (en) | A kind of preparation method and application of hollow fluorescent orange carbon nano-particles | |
| CN110257061A (en) | Method for preparing yellow fluorescence carbon dots using 2,3- diamino-pyridine and products thereof and application | |
| CN104555984B (en) | A kind of carbon quantum dot fluorescence labeling material with Ovum Gallus domesticus album as carbon source and its preparation method and application | |
| CN106370640B (en) | Carbon dots-gold cluster composite fluorescence nano-probe and its application | |
| CN111518552B (en) | Preparation of fluorine-containing graphene quantum dots and application of fluorine-containing graphene quantum dots as photodynamic therapy photosensitizer | |
| CN107529514A (en) | Adulterate the preparation method and applications of fluorine ion carbon point |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190419 Termination date: 20200120 |