CN107036991A - A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS - Google Patents
A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS Download PDFInfo
- Publication number
- CN107036991A CN107036991A CN201710179130.XA CN201710179130A CN107036991A CN 107036991 A CN107036991 A CN 107036991A CN 201710179130 A CN201710179130 A CN 201710179130A CN 107036991 A CN107036991 A CN 107036991A
- Authority
- CN
- China
- Prior art keywords
- chlorine dioxide
- abts
- chlorite
- concentration
- analysis method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 title claims abstract description 170
- 239000004155 Chlorine dioxide Substances 0.000 title claims abstract description 82
- 235000019398 chlorine dioxide Nutrition 0.000 title claims abstract description 82
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 title claims abstract description 72
- QBWCMBCROVPCKQ-UHFFFAOYSA-N chlorous acid Chemical compound OCl=O QBWCMBCROVPCKQ-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 238000004458 analytical method Methods 0.000 title claims abstract description 26
- JFBJUMZWZDHTIF-UHFFFAOYSA-N chlorine chlorite Inorganic materials ClOCl=O JFBJUMZWZDHTIF-UHFFFAOYSA-N 0.000 title claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 69
- 238000002835 absorbance Methods 0.000 claims abstract description 38
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 20
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 12
- 239000002253 acid Substances 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 31
- 229910001919 chlorite Inorganic materials 0.000 claims description 25
- 229910052619 chlorite group Inorganic materials 0.000 claims description 25
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 18
- 239000000460 chlorine Substances 0.000 claims description 18
- 229910052801 chlorine Inorganic materials 0.000 claims description 18
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 14
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 230000001186 cumulative effect Effects 0.000 claims description 4
- 239000008363 phosphate buffer Substances 0.000 claims description 4
- 239000008055 phosphate buffer solution Substances 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229910017604 nitric acid Inorganic materials 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- NHYCGSASNAIGLD-UHFFFAOYSA-N Chlorine monoxide Chemical compound Cl[O] NHYCGSASNAIGLD-UHFFFAOYSA-N 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- XTEGARKTQYYJKE-UHFFFAOYSA-M Chlorate Chemical compound [O-]Cl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-M 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000007800 oxidant agent Substances 0.000 description 5
- 230000001590 oxidative effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 150000003254 radicals Chemical class 0.000 description 4
- 238000004448 titration Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000790917 Dioxys <bee> Species 0.000 description 3
- 239000000645 desinfectant Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000033116 oxidation-reduction process Effects 0.000 description 2
- BULVZWIRKLYCBC-UHFFFAOYSA-N phorate Chemical compound CCOP(=S)(OCC)SCSCC BULVZWIRKLYCBC-UHFFFAOYSA-N 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241001550224 Apha Species 0.000 description 1
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 description 1
- -1 N, N- diethyl p-phenylenediamines ferrous ammonium sulfate Chemical class 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- DFGWJCZWKBGVOX-UHFFFAOYSA-N [Cl+].[O-2].[O-2].[Ti+4] Chemical compound [Cl+].[O-2].[O-2].[Ti+4] DFGWJCZWKBGVOX-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960003260 chlorhexidine Drugs 0.000 description 1
- 239000002384 drinking water standard Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides a kind of chlorine dioxide and chlorite rapid analysis method based on ABTS, and this method, which is crossed, takes water sample to be measured, and regulation pH value is 6.5, adds ABTS solution, determines absorbance of the solution under 405nm wavelength, calculates the concentration for obtaining chlorine dioxide；Chlorine dioxide in logical nitrogen stripping water sample, then adds acid for adjusting pH value to be 2, adds ABTS solution, determines absorbance of the solution under 405nm wavelength, calculates the concentration for obtaining chlorine dioxide.This method can be quick, accurate, economical, simple to operate carry out water quality chlorine dioxide and chloritic analysis.
Description
Technical field
The present invention relates to disinfectant field in analyzing water body, more particularly, to a kind of chlorine dioxide based on ABTS and
Chlorite rapid analysis method.
Background technology
Chlorine dioxide is generally acknowledged efficient, wide spectrum of new generation, the sterilization of safety, antistaling agent in the world, is chlorhexidine-containing disinfectant
Preferable substitute, is widely used all over the world.Meanwhile, disinfection by chlorine dioxide is nearly free from haloform, halogen
The DBPs such as acetic acid.And chlorite is the common accessory substance that disinfection by chlorine dioxide is produced, chlorine dioxide self property
Also it is unstable, easily it is decomposed into chlorite etc..What China promulgated for 2006《Standards for drinking water quality》Provide titanium dioxide
Limit value concentration is 0.1-0.8mg/L in the output water of chlorine, when using disinfection by chlorine dioxide, limit of the chlorite in drinking water
It is worth for 0.7mg/L.Therefore, it is necessary to chlorine dioxide and chloritic in periodic monitoring water when being carried out disinfection using chlorine dioxide
Concentration, to ensure water quality safety.
At present in Chinese GB5750-2006《Drinking water standard detection method》With U.S. APHA《Standard
Methods for the Examination of Water and Wastewater》In, the chlorine dioxide in drinking water is commonly used
Assay method be N, N- diethyl p-phenylenediamines ferrous ammonium sulfate titration (DPD-FAS methods) and iodimetric titration；Chlorite exists
Detection method be iodimetric titration and the chromatography of ions.Instrument needed for the chromatography of ions --- ion chromatograph is expensive, typically
Water factory does not have fund outfit, and comparatively DPD-FAS methods and instrument price required for iodimetric titration are relatively inexpensive but required when determining
Reagent is more and inconvenient on-site measurement.Also, many water factories are when using chlorine dioxide as disinfectant, by titanium dioxide
Chlorine and free chlorine are used in combination, and when using iodometric determination chlorite, chlorate and free chlorine can be produced to measurement result
Raw influence.
The content of the invention
It is fast that the present invention provides a kind of quick, accurate, economical, simple to operate chlorine dioxide and chlorite based on ABTS
Fast analysis method.
In order to reach above-mentioned technique effect, technical scheme is as follows：
A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS, including chlorine dioxide analysis method and
Chloritic analysis method；
The analysis method of the chlorine dioxide is：
Water sample to be measured is taken, regulation pH value is 6.5, adds ABTS solution, determine absorbance of the solution under 405nm wavelength
A1, then pass through formula：Chlorine dioxide concentration=2.37A1/b×V2/V1Calculate the concentration of chlorine dioxide；
The chloritic analysis method：
Water sample to be measured is taken, then the chlorine dioxide led in nitrogen stripping water sample adds acid for adjusting pH value to be 2, add ABTS molten
Liquid, determines absorbance A of the solution under 405nm wavelength2, chlorite concentration=0.53A2/b×V2/V1, wherein, A1For pH
The absorbance measured under the conditions of 6.5；A2For the absorbance measured under the conditions of pH 2.0；B is cuvette light path；V1For institute's water sampling
Volume；V2To add the cumulative volume after various reagents.
In the present invention, ABTS is a kind of substrate of catalase, and its oxidation-reduction potential is 0.68V, easily with oxidation
Electro transfer, the green free radical ABTS of generation occur for agent reaction·+。ABTS·+Stability is strong, and half-life period is 47 at ambient temperature
Hour, at 4 DEG C, half-life period is 357 hours, therefore, at ambient temperature the attenuation rate in 5 hours<5%, 24 hours at 4 DEG C
Interior attenuation rate<3%.Also, ABTS·+Absorbance when 405nm molar absorption coefficient is in pH 6.5 and pH 2 is respectively
28500M-1cm-1And 31600M-1cm-1.It therefore, it can according to reacted ABTS·+Absorbance, and oxidant and ABTS it is anti-
The quantitative relationship answered, directly calculates the concentration of oxidant, without bioassay standard curve.
In pH 6.5, ABTS only generates ABTS free radicals (ABTS with chlorine dioxide reaction·+), and chlorite and chlorine
Hydrochlorate does not react (formula 1) with ABTS；In pH 2, chlorite and chlorine dioxide can be with ABTS reaction generations ABTS·+, and
Chlorate does not react with ABTS.
ClO2+ABTS→ClO2 -+ABTS·+ (1)
ClO2 -+4ABTS+4H+→Cl-+4ABTS·++2H2O (2)
Around this principle, in pH=2, with nitrogen stripping chlorine dioxide, the chlorite in water can be carried out single
Solely determine.Chlorine dioxide and chlorite react the ABTS of generation with ABTS·+For green, pass through ultraviolet-uisible spectrophotometer
The absorbance that it is determined in 405nm determines concentration.
Further, pH is adjusted to 6.5 using phosphate buffer solution, and makes phosphate concn be 12mM；Using sulfuric acid,
One or more in nitric acid, hydrochloric acid, phosphoric acid adjust chloritic pH to 2 to be determined.
Further, absorbance A is determined1Process it is as follows：
Water sample 21mL to be measured is taken, the phosphate buffer solutions of pH 6.5 that 3mL concentration is 0.1M are added, adding 1mL concentration is
1g/L ABTS solution, then absorbance of the determination sample under 405nm wavelength.
Further, absorbance A is determined2Process it is as follows：
Water sample 21mL to be measured is taken, the nitrogen 3min that flow velocity is 20ml/min is passed through, the salt that 3mL equivalent concentration is 0.3M is added
Acid solution, adds the ABTS solution that 1mL concentration is 1g/L, then absorbance of the determination sample under 405nm wavelength.
Further, when there is free chlorine simultaneously in water sample to be measured, 5g/L glycine 0.2mL is added, to eliminate certainly
The influence determined by chlorine to chlorine dioxide and chlorite.
Further, the concentration range that this method determines chlorine dioxide in water is 0.15-2.65mg/L, chloritic dense
Degree scope is 0.07-0.7mg/L；If chlorine dioxide and chlorite are more than this concentration range in water sample to be measured, pure water need to be used
It is diluted to the concentration range.
The chlorine dioxide and chloritic concentration in water sample are determined, is calculated by below equation：
Chlorine dioxide concentration (mg/L)=A1/(28500×b)×67.5×1000×V2/V1
=2.37A1/b×V2/V1 (3)
Chlorite concentration (mg/L)=A/ (31600 × b)/4 × 67.5 × 1000 × V2/V1
=0.53A2/b×V2/V1 (4)
Wherein, A1For the absorbance measured under the conditions of pH 6.5；A2For the absorbance measured under the conditions of pH 2；B is cuvette
Light path (cm)；V1For the volume (mL) of institute's water sampling；V2To add the cumulative volume (mL) after various reagents.
Compared with prior art, the beneficial effect of technical solution of the present invention is：
The present invention is by taking water sample to be measured, and regulation pH value is 6.5, adds ABTS solution, determines solution under 405nm wavelength
Absorbance, the concentration of chlorine dioxide is obtained by standard curve；Chlorine dioxide in logical nitrogen stripping water sample, then acid adding adjust
It is 2 to save pH value, adds ABTS solution, determines absorbance of the solution under 405nm wavelength, chlorine dioxide is obtained by standard curve
Concentration.This method can be quick, accurate, economical, simple to operate carry out water quality chlorine dioxide and chloritic analysis.
Brief description of the drawings
Fig. 1 is that and chlorite and chlorate are to dioxy in 6.5 times chlorine dioxide standard curves of pH based on ABTS methods
Change the interference of chlorine measurement；
Fig. 2 is that chlorite measure is done in 2 times chlorite standard curves of pH, and chlorate based on ABTS methods
Disturb.
Embodiment
Accompanying drawing being given for example only property explanation, it is impossible to be interpreted as the limitation to this patent；
In order to more preferably illustrate the present embodiment, some parts of accompanying drawing have omission, zoomed in or out, and do not represent actual product
Size；
To those skilled in the art, it is to be appreciated that some known features and its explanation, which may be omitted, in accompanying drawing
's.
Technical scheme is described further with reference to the accompanying drawings and examples.
The present invention proposes that one kind joins the two of nitrogen-two (3- ethyls-benzothiazole -6- sulfonic acid) di-ammonium salts (ABTS) based on 2,2-
Chlorine monoxid and chlorite rapid analysis method.
ABTS is a kind of substrate of catalase, and its oxidation-reduction potential is 0.68V, is easily occurred with oxidant reaction
Electro transfer, the green free radical ABTS of generation·+。ABTS·+Stability is strong, and half-life period is 47 hours at ambient temperature, at 4 DEG C
When half-life period be 357 hours, therefore, the attenuation rate in 5 hours at ambient temperature<5%, attenuation rate at 4 DEG C in 24 hours<
3%.Also, ABTS·+Absorbance when 405nm molar absorption coefficient is in pH 6.5 and pH 2 is respectively 28500M-1cm-1
And 31600M-1cm-1.It therefore, it can according to reacted ABTS·+Absorbance, and the quantitative pass that oxidant reacts with ABTS
System, directly calculates the concentration of oxidant, without bioassay standard curve.
Chlorine dioxide is surveyed in 6.5 times chlorine dioxide standard curves of pH, and chlorite and chlorate based on ABTS methods
Fixed interference is as shown in Figure 1；Chlorite is determined in 2 times chlorite standard curves of pH, and chlorate based on ABTS methods
Interference it is as shown in Figure 2.
The technical scheme is that：
A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS, comprising：(1) the analysis side of chlorine dioxide
Method：Water sample to be measured is taken, regulation pH value is 6.5, adds ABTS solution, determine absorbance of the solution under 405nm wavelength, pass through mark
Directrix curve obtains the concentration of chlorine dioxide；(2) chloritic analysis method：Water sample to be measured is taken, is led in nitrogen stripping water sample
Chlorine dioxide, then adds acid for adjusting pH value to be 2, adds ABTS solution, determines absorbance of the solution under 405nm wavelength, passes through
Standard curve obtains the concentration of chlorine dioxide.
Using phosphate buffer solution regulation pH to 6.5 (phosphate concn=12mM)；Using sulfuric acid, nitric acid, hydrochloric acid, phosphorus
One or more of regulations in acid determine chloritic pH to 2.
The present invention principle be：In pH 6.5, ABTS only generates ABTS free radicals (ABTS with chlorine dioxide reaction·+),
And chlorite and chlorate do not react (formula 1) with ABTS；In pH 2, chlorite and chlorine dioxide can react with ABTS
Generate ABTS·+, and chlorate does not react with ABTS.
ClO2+ABTS→ClO2 -+ABTS·+ (1)
ClO2 -+4ABTS+4H+→Cl-+4ABTS·++2H2O (2)
Around this principle, in pH 2, with nitrogen stripping chlorine dioxide, the chlorite in water can be carried out independent
Determine.Chlorine dioxide and chlorite react the ABTS of generation with ABTS·+For green, surveyed by ultraviolet-uisible spectrophotometer
Fixed its determines concentration in 405nm absorbance.
General operation is to take 21mL to contain chlorine dioxide and chloritic water sample, if chlorine dioxide in water sample to be measured
It is more than this method detection range with chlorite, pure water can be added to be diluted to the concentration range.Take 0.1M phosphate buffer solution
3mL makes water sample pH to be measured be 6.5, then 1g/L ABTS solution 1mL is added water sample, determines its absorbance A1；Take 21mL same again
Sample contains chlorine dioxide and chloritic water sample, is passed through chlorine dioxide in nitrogen stripping water sample, adds 0.3M hydrochloric acid 3mL and makes
Water sample pH to be measured is 2, takes 1g/L ABTS solution 1mL, determines its absorbance A2。
When also there is free chlorine simultaneously in water sample to be measured, 5g/L glycine solution 0.2mL need to be added before the assay, eventually
Only all free chlorine, to eliminate the influence that free chlorine is determined to chlorine dioxide and chlorite.
The optimum range that this method can measure the concentration of chlorine dioxide is 0.15-2.65mg/L, chloritic concentration
Optimum range is 0.07-0.7mg/L.If chlorine dioxide and chlorite are more than this concentration range in water sample to be measured, it can adopt
The concentration range is diluted to pure water.
The chlorine dioxide and chloritic concentration in water sample are determined, is calculated by below equation：
Chlorine dioxide concentration (mg/L)=A1/(28500×b)×67.5×1000×V2/V1
=2.37A1/b×V2/V1 (3)
Chlorite concentration (mg/L)=A2/(31600×b)/4×67.5×1000×V2/V1
=0.53A2/b×V2/V1 (4)
Wherein, A1For the absorbance measured under the conditions of pH 6.5；A2For the absorbance measured under the conditions of pH 2.0；B is colorimetric
Ware light path (cm)；V1For the volume (mL) of institute's water sampling；V2To add the cumulative volume (mL) after various reagents.
Two groups of experiments have been carried out using this method：
Experiment one：
When containing chlorine dioxide and chlorite in water sample, to the measure of chlorine dioxide：
21mL is taken to contain chlorine dioxide and chloritic water sample, the 0.1mol/L phosphate buffer solutions 3mL of addition makes to treat
It is 6.5 to survey water sample pH, and adds 1g/L ABTS solution 1mL, determines its absorbance A 1.Formula (3) is substituted into, is obtained two in water sample
The concentration of chlorine monoxid.
When containing chlorine dioxide and chlorite in water sample, determined to chloritic：
Take 21mL to contain chlorine dioxide and chloritic water sample, be passed through dioxy in 20mL/min nitrogen 3min stripping water samples
Change chlorine, add 0.3M hydrochloric acid 3mL up to water sample pH to be measured is 2, take 1g/L ABTS solution 1mL, determine its absorbance A2.Generation
Enter formula (4), obtain chloritic concentration in water sample.
Experiment two：
When chlorine dioxide, chlorite and free chlorine coexist, to the measure of chlorine dioxide：
21mL is taken to contain after chlorine dioxide and chloritic water sample, addition 5g/L glycine 0.2mL terminations are all
Free chlorine, the 0.1mol/L phosphate buffer solutions 3mL then added makes water sample pH to be measured be 6.5, and it is molten to add 1g/L ABTS
Liquid 1mL, determines its absorbance A1.Formula (3) is substituted into, the concentration of chlorine dioxide in water sample is obtained.
When chlorine dioxide, chlorite and free chlorine coexist, determined to chloritic：
Take 21mL to contain chlorine dioxide and chloritic water sample, be passed through dioxy in 20mL/min nitrogen 3min stripping water samples
Change chlorine, addition 5g/L glycine 0.2mL terminates all free chlorine, then adds 0.3M hydrochloric acid 3mL until water sample to be measured
PH is 2, takes 1g/L ABTS solution 1mL, determines its absorbance A2.Formula (4) is substituted into, obtains chloritic dense in water sample
Degree.
The same or analogous part of same or analogous label correspondence；
Position relationship is used for being given for example only property explanation described in accompanying drawing, it is impossible to be interpreted as the limitation to this patent；
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not pair
The restriction of embodiments of the present invention.For those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.It is all this
Any modifications, equivalent substitutions and improvements made within the spirit and principle of invention etc., should be included in the claims in the present invention
Protection domain within.
Claims (6)
1. a kind of chlorine dioxide and chlorite rapid analysis method based on ABTS, it is characterised in that including chlorine dioxide
Analysis method and chloritic analysis method；
The analysis method of the chlorine dioxide is：
Water sample to be measured is taken, regulation pH value is 6.5, adds ABTS solution, determine absorbance A of the solution under 405nm wavelength1, then
Pass through formula：Chlorine dioxide concentration=2.37A1/b×V2/V1Calculate the concentration of chlorine dioxide；
The chloritic analysis method：
Water sample to be measured is taken, then the chlorine dioxide led in nitrogen stripping water sample adds acid for adjusting pH value to be 2, add ABTS solution, surveys
Determine absorbance A of the solution under 405nm wavelength2, chlorite concentration=0.53A2/b×V2/V1, wherein, A1For the conditions of pH 6.5
Under the absorbance that measures；A2For the absorbance measured under the conditions of pH 2.0；B is cuvette light path；V1For the volume of institute's water sampling；
V2To add the cumulative volume after various reagents.
2. chlorine dioxide and chlorite rapid analysis method according to claim 1 based on ABTS, it is characterised in that
PH is adjusted to 6.5 using phosphate buffer solution, and makes phosphate concn be 12mM；Using in sulfuric acid, nitric acid, hydrochloric acid, phosphoric acid
One or more adjust chloritic pH to 2 to be determined.
3. chlorine dioxide and chlorite rapid analysis method according to claim 2 based on ABTS, it is characterised in that
Determine absorbance A1Process it is as follows：
Water sample 21mL to be measured is taken, the phosphate buffer solutions of pH 6.5 that 3mL concentration is 0.1M are added, 1mL concentration is added for 1g/L
ABTS solution, then absorbance of the determination sample under 405nm wavelength.
4. chlorine dioxide and chlorite rapid analysis method according to claim 2 based on ABTS, it is characterised in that
Determine absorbance A2Process it is as follows：
Water sample 21mL to be measured is taken, the nitrogen 3min that flow velocity is 20ml/min is passed through, adds 3mL equivalent concentration molten for 0.3M hydrochloric acid
Liquid, adds the ABTS solution that 1mL concentration is 1g/L, then absorbance of the determination sample under 405nm wavelength.
5. the chlorine dioxide and chlorite rapid analysis method based on ABTS according to claim any one of 3-4, its
Being characterised by, when there is free chlorine simultaneously in water sample to be measured, 5g/L glycine 0.2mL is added, to eliminate free chlorine to two
The influence that chlorine monoxid and chlorite are determined.
6. chlorine dioxide and chlorite rapid analysis method according to claim 1 based on ABTS, it is characterised in that
The concentration range that this method determines chlorine dioxide in water is 0.15-2.65mg/L, and chloritic concentration range is 0.07-
0.7mg/L；If chlorine dioxide and chlorite are more than this concentration range in water sample to be measured, the concentration model need to be diluted to using pure water
Enclose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710179130.XA CN107036991A (en) | 2017-03-23 | 2017-03-23 | A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710179130.XA CN107036991A (en) | 2017-03-23 | 2017-03-23 | A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107036991A true CN107036991A (en) | 2017-08-11 |
Family
ID=59533321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710179130.XA Withdrawn CN107036991A (en) | 2017-03-23 | 2017-03-23 | A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107036991A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101318728A (en) * | 2008-07-16 | 2008-12-10 | 中山大学 | Ultraviolet light catalysis disinfection method by chlorine dioxide for water |
| CN105527280A (en) * | 2014-09-30 | 2016-04-27 | 深圳市水务(集团)有限公司 | Method for detecting chlorine dioxide and chlorite concentration in water through LGB-HRP quantitative injection |
-
2017
- 2017-03-23 CN CN201710179130.XA patent/CN107036991A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101318728A (en) * | 2008-07-16 | 2008-12-10 | 中山大学 | Ultraviolet light catalysis disinfection method by chlorine dioxide for water |
| CN105527280A (en) * | 2014-09-30 | 2016-04-27 | 深圳市水务(集团)有限公司 | Method for detecting chlorine dioxide and chlorite concentration in water through LGB-HRP quantitative injection |
Non-Patent Citations (1)
| Title |
|---|
| ULRICH PINKERNELL 等: "Methods For The Photometric Determination Of Reactive Bromine And Chlorine Species With ABTS", 《WATER RESEARCH》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Huo et al. | A fluorescein-based highly specific colorimetric and fluorescent probe for hypochlorites in aqueous solution and its application in tap water | |
| Klassen et al. | H2O2 determination by the I3-method and by KMnO4 titration | |
| US8065906B2 (en) | Detection of free chlorine in water | |
| Yang et al. | Simple 1, 8-diaminonaphthalene-based fluorescence chemosensor for hypochlorites and its practical application | |
| Connick et al. | Kinetics and mechanism of the oxidation of HSO3-by O2. 1. the uncatalyzed reaction | |
| CN102564982A (en) | Method for determining and correcting cyanide | |
| CN107036991A (en) | A kind of chlorine dioxide and chlorite rapid analysis method based on ABTS | |
| CN110174397B (en) | Application of colorimetric probe | |
| US9746451B2 (en) | Kinetic chlorine measurement | |
| RU2231060C2 (en) | Azodye-base aqueous solution, method for its preparing and its using | |
| US5155048A (en) | Organic reagent for the colorimetric detection of chlorine and ozone in drinking water | |
| JP2017032503A (en) | Residual chlorine measurement system, residual chlorine measurement method, and program | |
| US9513227B2 (en) | Method for quantitative determination of oxidant and apparatus for quantitative determination of oxidant | |
| Kramer et al. | Determination of microquantities of iodine in water solution by amperometric titrations | |
| Harp et al. | Spectrophotometric determination of chlorine dioxide | |
| Dahlgren | Spectrophotometric Determination of Ethyl-, Diethyl-and Triethylamine in Aqueous Solution. | |
| JP2006170897A (en) | Method and apparatus for measuring chemical oxygen demand (cod) | |
| CN103383356A (en) | Method for rapidly determining chlorates in drinking water | |
| KR20180091180A (en) | Method and Test Kit For Dectecting Concentration of Hydrogen Peroxide | |
| Lambert et al. | Induced colorimetric method for carbon monoxide | |
| CN103018184B (en) | A kind of method measuring underwater trace bromate | |
| Olenin et al. | Sols of silver nanoparticles as analytical reagents for the determination of active chlorine in water samples by spectrophotometry and photometric titration | |
| JP6953521B2 (en) | A method for measuring the concentration of a chemical species using a reagent baseline | |
| JP6954387B2 (en) | Continuous measurement method and equipment for starch concentration in water | |
| Yoshinaga et al. | Polyvinyl alcohol as a useful indicator on iodometry (II): temperature dependence of iodine recovery and the correction method of iodine concentration in the lower detection limit region |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170811 |