CN107034248A - A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine - Google Patents
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine Download PDFInfo
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Abstract
The present invention relates to the technology for producing field of phosphatidylserine, a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine is specifically related to.By the way that phospholipase D is carried on into high adsorbed gas gel porous material, so as to improve the reactivity and conversion catalyst efficiency of phospholipase D so that complete the catalytic synthesis of phosphatidylserine in a short time；Further, by carrying out the reaction of organic phase, aqueous phase under high pressure in narrow flow road, not only reaction efficiency is high, and narrow flow is homogenized to fluid and reacted, and reaction is uniform, it is easy to stability contorting.Compared to long-time catalytic reaction in a kettle., its efficiency, stability are substantially improved.Gained phosphatidylserine can be used as dietetic product or functional food, activation man memory power and cognitive function.
Description
Technical field
The present invention relates to the technology for producing field of phosphatidylserine, a kind of continuous high-efficient enzymatic conversion is specifically related to
The method of fabricated food phosphatidylserine.
Background technology
Phosphatidylserine（Phosphatidylserine, PS）Also known as composite nerve is sour, and its function is mainly improvement god
Through cell function, the conduction of nerve impulse is adjusted, promotes brain memory function, because it has very strong lipophilicity, after absorption
Blood-brain barrier can be run through and enter brain, vascular smooth muscle cells of releiving are played, increase the effect of brain blood supply.It no matter
Be that certainly, supplement phosphatidylserine can improve long-term memory as medicine or health food, it is long-term cognitive and
Free talk and logicality speech ability.
Phosphatidylserine（PS）Have become many American-European countries's research losses of memory, mood, cognitive behavior and study
The problem of the relevant clinical test of ability.Clinical test results are shown, after supplement phosphatidylserine, on cognitive and memory
Parameter significantly improves.Certainly, supplement phosphatidylserine can improve long-term memory, long-term cognition and freely talk
Tuhe logicality speech ability.Phosphatidylserine（PS）It can be proportionally added into meals, can be by enteron aisle effectively
Promptly it is absorbed into blood, and the finally and successfully nerve cell that intracerebral is reached through blood-brain barrier.
Phosphatidylserine（PS）It can be synthesized by the mankind or animal self-condition, can also be from plant extract, or can
Produced using biocatalysis technology from soybean lecithin.By using phospholipase D（PLDs）Phosphatidyl transfer
（transphosphatidylation）Reaction, easily can be modified the head base of phosphatide.Therefore, phosphatidylserine can
Pass through PLD catalytic productions from phosphatidyl choline or any other mixture of phospholipids with serine.
Existing phosphatidylserine is mainly used from extraction method in the brain tissue, hepatic tissue of animal, or using biological
Enzyme synthesizes for catalyst.Wherein extraction method needs substantial amounts of animal tissue, and extraction process is used compared with multi-solvent, and efficiency is low,
Process for separating and purifying is complicated, and separating difficulty is big；Biological enzyme synthetic method can batch synthetic phospholipid acyl serine.But due to enzyme
Catalytic capability it is limited, usual generated time is long, and yield is low.
The content of the invention
In view of the shortcomings of the prior art, the present invention provides a kind of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine
Method, its significant characteristic is that phospholipase D is carried on into high-absorption material, and the reactivity and conversion for improving phospholipase D are urged
Change efficiency, the enzyme and serine that pre-activate is handled are distributed in aqueous phase, by soybean lecithin（PC）It is dissolved in organic solvent, in advance
Organic phase and aqueous phase are mixed in runner after heat, during runner continuously flows, the generation efficient catalytic of pre-activate enzyme is made
With being formed continuously phosphatidylserine, it is high that it reacts continuous, efficiency high, yield.Gained phosphatidylserine is suitable for nutrition
Product or functional food, activation man memory power and cognitive function.
To achieve the above object, using following concrete technical scheme：
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, it is characterised in that specific preparation method is such as
Under：
（1）By phospholipase D and porous material with mass ratio 1:3-5 disperses, application of vacuum 1-2h so that phospholipase D uniform load
In the micropore of porous material, activation of phospholipase D systems are obtained；Wherein, described porous material is aerosil, stone
At least one of black alkene aeroge, carbon aerogels, titania aerogel；
（2）By step（1）Obtained activation of phospholipase D systems, serine are with mass ratio 1:20-50 disperses under ul-trasonic irradiation
5-10min, obtains mixed system I；
（3）By soybean lecithin and organic solvent with mass ratio 1:3-5 is well mixed, and obtains mixed system II；
（4）Mixed system I, mixed system II are respectively charged into pressurized tank, the pressurized tank of mixed system I maintains constant pressure 0.1-
0.3MPa, the pressurized tank of mixed system II maintains constant pressure 0.5-1MPa, preheats as 60-80 DEG C, by control pressure tank valve, will
Mixed system I, mixed system II are with volume ratio 1:3-5 flow at high speed is mixed, and rapidly by narrow flow road, in narrow flow road continuous stream
During dynamic, pre-activate phospholipase D system produces efficient catalytic effect, and serine and soybean lecithin continuously are catalyzed and synthesized into phosphatide
Acyl serine；
（5）Phosphatidylserine crude product is continuously collected in narrow flow road rear end, organic phase is washed with water, vacuum sloughs organic solvent,
Absolute ethyl alcohol insoluble matter is further isolated with absolute ethyl alcohol, vacuum drying obtains phosphatidylserine.
It is preferred that, step（1）The specific surface area of the porous material is more than 700m2/ g, pore radius is 1-3nm, preferably
Phospholipase D is set to be in optimal activation state, so as to realize quick catalysis synthetic phospholipid acyl serine.
It is preferred that, step（2）The serine is Serine, and concentration is 0.3-0.35g/ml.
It is preferred that, step（3）The content of the phosphorus level in soya lecithin phosphatidylcholine is 5 ~ 35%, preferably 10 ~ 35%.
The present invention does not have special requirement to the species of organic solvent, as long as the dissolving of soybean lecithin can be realized,
Can be with organic solvent known to those skilled in the art, organic solvent is C5 ~ C12 alkane, ether, ketones solvent as described
At least one of with esters solvent.
It is preferred that, step（3）The organic solvent is at least one in cyclohexanone, petroleum ether, acetone, ethyl acetate etc.
Kind.
It is preferred that, step（3）The narrow flow road is internal diameter 3-5mm, long 2-3m stainless steel pipes.
It is preferred that, step（3）The cross section in the narrow flow road is cylindrical shape or triangle；It is furthermore preferred that the narrow flow road
Set for cylindrical shape is alternate with triangle so that serine is fully contacted with soybean lecithin, activation of phospholipase D systems, improve anti-
Answer efficiency.
It is preferred that, step（3）Described narrow flow road periphery sets water-cooling system, and narrow flow channel temp is maintained into 60-80 DEG C.
The present invention is obtained by the way that reactant is reacted under conditions of flowing, therefore, and the flow velocity of reactant is to reaction
Influence it is very big, when the flow velocity of reactant is very fast, then reactant can be caused to react completely, reaction efficiency is low, the production of product
Rate is low, and when the flow velocity of reactant is slower, then causes serine and the contact force of soybean lecithin, activation of phospholipase D systems to reduce,
Equally reduce production efficiency；It is preferred that, in step（4）, the flow velocity in narrow flow road of fluid is 0.5 ~ 1 m/s.
According to the present invention, emulsifying agent can promote the generation of phosphatidylserine, in another preferred implementation of the present invention
In mode, also contain emulsifying agent in the mixed system.Step（3）In, it is the further reaction efficiency for improving soybean lecithin, molten
Solution dispersiveness, preferably uses glycerin monostearate and is emulsified.
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine of the present invention, by the way that phospholipase D is born
High adsorbed gas gel porous material is loaded in, so as to improve the reactivity and conversion catalyst efficiency of phospholipase D so that in the short time
The interior catalytic synthesis for completing phosphatidylserine；Further, by carrying out organic phase, aqueous phase under high pressure in the narrow flow road
Reaction, not only reaction efficiency is high, and narrow flow is homogenized to fluid and reacted, and reaction is uniform, it is easy to stability contorting.Compared in reaction
Long-time catalytic reaction in kettle, its efficiency, stability are substantially improved.Gained phosphatidylserine can be used as dietetic product or function
Property food, activation man memory power and cognitive function.
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine of the present invention, compared with prior art,
The characteristics of it is protruded and excellent effect are：
1st, phospholipase D is carried on high absorption aerogel material, improves the reactivity and conversion catalyst efficiency of phospholipase D.
2nd, using narrow flow road, serine and soybean lecithin, the abundant high-efficient contact of activation of phospholipase D systems under high pressure, from
And realize that continuous high-efficient prepares phosphatidylserine, it reacts continuous, efficiency high, yield height.
3rd, the inventive method technique is simple, preparation process cleanliness without any pollution, preparation efficiency are high, is suitable for large-scale production.
Embodiment
Below by way of embodiment, the present invention is described in further detail, but this should not be interpreted as to the present invention
Scope be only limitted to following example.In the case where not departing from above method thought of the present invention, according to ordinary skill
Various replacements or change that knowledge and customary means are made, should be included in the scope of the present invention.
Embodiment 1
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, it is characterised in that specific preparation method is such as
Under：
（1）By phospholipase D and porous material aerosil with mass ratio 1:3 disperse, application of vacuum 1h so that phosphatidase
D uniform loads obtain activation of phospholipase D systems in the micropore of porous material；
（2）By step（1）Obtained activation of phospholipase D systems, serine are with mass ratio 1:20 disperse under ul-trasonic irradiation
10min, obtains mixed system I；The serine is Serine, and concentration is 0.3g/ml；
（3）By the content of phosphatidyl choline for 10% soybean lecithin and organic solvent cyclohexanone with mass ratio 1:3 are well mixed,
And the emulsifying agent glycerin monostearate of soybean lecithin quality 1% is added, it is uniformly dispersed and obtains mixed system II；
（4）Mixed system I, mixed system II are respectively charged into pressurized tank, the pressurized tank of mixed system I maintains constant pressure 0.1MPa,
The pressurized tank of mixed system II maintains constant pressure 0.5MPa, preheats as 80 DEG C, by control pressure tank valve, by mixed system I, mixes
Zoarium system II is with volume ratio 1:3 flow at high speed are mixed, and rapidly pass through narrow flow road, during narrow flow road continuously flowing, pre- work
Change phospholipase D system and produce efficient catalytic effect, serine and soybean lecithin are continuously catalyzed and synthesized into phosphatidylserine；It is described
Narrow flow road is cylindrical shape, and narrow flow road is internal diameter 3mm, long 2m stainless steel pipes;So that serine and soybean lecithin, activation phosphatide
Enzyme D systems are fully contacted, and improve reaction efficiency；Narrow flow road periphery sets water-cooling system, and narrow flow channel temp is maintained into 80 DEG C；
（5）Phosphatidylserine crude product is continuously collected in narrow flow road rear end, organic phase is washed with water, vacuum sloughs organic solvent,
Absolute ethyl alcohol insoluble matter is further isolated with absolute ethyl alcohol, vacuum drying obtains phosphatidylserine.
The yield that the embodiment 1 prepares phosphatidylserine reaches 25kg/h, and dry phosphatidylserine content is 72%
Embodiment 2
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, it is characterised in that specific preparation method is such as
Under：
（1）By phospholipase D and porous material graphene aerogel with mass ratio 1:4 disperse, application of vacuum 1.5h so that phosphatidase
D uniform loads obtain activation of phospholipase D systems in the micropore of porous material；
（2）By step（1）Obtained activation of phospholipase D systems, serine are with mass ratio 1:20 disperse under ul-trasonic irradiation
10min, obtains mixed system I；The serine is Serine, and concentration is 0.35g/ml；
（3）By the content of phosphatidyl choline for 15% soybean lecithin and organic solvent petroleum ether with mass ratio 1:5 are well mixed,
And the emulsifying agent glycerin monostearate of soybean lecithin quality 2% is added, it is uniformly dispersed and obtains mixed system II；
（4）Mixed system I, mixed system II are respectively charged into pressurized tank, the pressurized tank of mixed system I maintains constant pressure 0.2MPa,
The pressurized tank of mixed system II maintains constant pressure 0.5MPa, preheats as 80 DEG C, by control pressure tank valve, by mixed system I, mixes
Zoarium system II is with volume ratio 1:3 flow at high speed are mixed, and rapidly pass through narrow flow road, during narrow flow road continuously flowing, pre- work
Change phospholipase D system and produce efficient catalytic effect, serine and soybean lecithin are continuously catalyzed and synthesized into phosphatidylserine；It is described
Narrow flow road, which is that cylindrical shape is alternate with triangle, to be set, and narrow flow road is internal diameter 3mm, long 2.5m stainless steel pipes;So that serine
Fully contacted with soybean lecithin, activation of phospholipase D systems, improve reaction efficiency；Narrow flow road periphery sets water-cooling system, by narrow flow
Channel temp maintains 80 DEG C；
（5）Phosphatidylserine crude product is continuously collected in narrow flow road rear end, organic phase is washed with water, vacuum sloughs organic solvent,
Absolute ethyl alcohol insoluble matter is further isolated with absolute ethyl alcohol, vacuum drying obtains phosphatidylserine.
The yield that the embodiment 2 prepares phosphatidylserine reaches 28kg/h, and dry phosphatidylserine content is
75.6%
Embodiment 3
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, it is characterised in that specific preparation method is such as
Under：
（1）By phospholipase D and porous material carbon aerogels with mass ratio 1:3 disperse, application of vacuum 2h so that phospholipase D is uniform
It is supported in the micropore of porous material, obtains activation of phospholipase D systems；
（2）By step（1）Obtained activation of phospholipase D systems, serine are with mass ratio 1:50 disperse under ul-trasonic irradiation
10min, obtains mixed system I；The serine is Serine, and concentration is 0.3g/ml；
（3）By the content of phosphatidyl choline for 5 ~ 35% soybean lecithin and organic solvent ethyl acetate with mass ratio 1:5 mixing are equal
It is even, and soybean lecithin quality 1-2% emulsifying agent glycerin monostearate is added, it is uniformly dispersed and obtains mixed system II；
（4）Mixed system I, mixed system II are respectively charged into pressurized tank, the pressurized tank of mixed system I maintains constant pressure 0.3MPa,
The pressurized tank of mixed system II maintains constant pressure 1MPa, preheats as 60 DEG C, by control pressure tank valve, by mixed system I, mixing
System II is with volume ratio 1:3 flow at high speed are mixed, and rapidly pass through narrow flow road, during narrow flow road continuously flowing, pre-activate
Phospholipase D system produces efficient catalytic effect, and serine and soybean lecithin continuously are catalyzed and synthesized into phosphatidylserine；It is described narrow
Runner, which is that cylindrical shape is alternate with triangle, to be set, and narrow flow road is internal diameter 5mm, long 3m stainless steel pipes;So that serine with it is big
Fabaceous Lecithin, activation of phospholipase D systems are fully contacted, and improve reaction efficiency；Narrow flow road periphery sets water-cooling system, by narrow flow road temperature
Degree maintains 80 DEG C；
（5）Phosphatidylserine crude product is continuously collected in narrow flow road rear end, organic phase is washed with water, vacuum sloughs organic solvent,
Absolute ethyl alcohol insoluble matter is further isolated with absolute ethyl alcohol, vacuum drying obtains phosphatidylserine.
The yield that the embodiment 3 prepares phosphatidylserine reaches 29kg/h, and dry phosphatidylserine content is
74.8%
Embodiment 4
A kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, it is characterised in that specific preparation method is such as
Under：
（1）By phospholipase D and porous material titania aerogel with mass ratio 1:3 disperse, application of vacuum 2h so that phosphatidase
D uniform loads obtain activation of phospholipase D systems in the micropore of porous material；
（2）By step（1）Obtained activation of phospholipase D systems, serine are with mass ratio 1:20 disperse under ul-trasonic irradiation
5min, obtains mixed system I；The serine is Serine, and concentration is 0.3g/ml；
（3）By the content of phosphatidyl choline for 5 ~ 35% soybean lecithin and organic solvent-acetone with mass ratio 1:3 are well mixed,
And soybean lecithin quality 1-2% emulsifying agent glycerin monostearate is added, it is uniformly dispersed and obtains mixed system II；
（4）Mixed system I, mixed system II are respectively charged into pressurized tank, the pressurized tank of mixed system I maintains constant pressure 0.3MPa,
The pressurized tank of mixed system II maintains constant pressure 1MPa, preheats as 80 DEG C, by control pressure tank valve, by mixed system I, mixing
System II is with volume ratio 1:4 flow at high speed are mixed, and rapidly pass through narrow flow road, during narrow flow road continuously flowing, pre-activate
Phospholipase D system produces efficient catalytic effect, and serine and soybean lecithin continuously are catalyzed and synthesized into phosphatidylserine；It is described narrow
Runner, which is that cylindrical shape is alternate with triangle, to be set, and narrow flow road is internal diameter 4mm, long 2m stainless steel pipes;So that serine with it is big
Fabaceous Lecithin, activation of phospholipase D systems are fully contacted, and improve reaction efficiency；Narrow flow road periphery sets water-cooling system, by narrow flow road temperature
Degree maintains 80 DEG C；
（5）Phosphatidylserine crude product is continuously collected in narrow flow road rear end, organic phase is washed with water, vacuum sloughs organic solvent,
Absolute ethyl alcohol insoluble matter is further isolated with absolute ethyl alcohol, vacuum drying obtains phosphatidylserine.
The yield that the embodiment 4 prepares phosphatidylserine reaches 30kg/h, and dry phosphatidylserine content is
75%。
Claims (10)
1. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, it is characterised in that specific preparation method
It is as follows：
（1）By phospholipase D and porous material with mass ratio 1:3-5 disperses, application of vacuum 1-2h so that phospholipase D uniform load
In the micropore of porous material, activation of phospholipase D systems are obtained；Wherein, described porous material is aerosil, stone
At least one of black alkene aeroge, carbon aerogels, titania aerogel；
（2）By step（1）Obtained activation of phospholipase D systems, serine are with mass ratio 1:20-50 disperses under ul-trasonic irradiation
5-10min, obtains mixed system I；
（3）By soybean lecithin and organic solvent with mass ratio 1:3-5 is well mixed, and obtains mixed system II；
（4）Mixed system I, mixed system II are respectively charged into pressurized tank, the pressurized tank of mixed system I maintains constant pressure 0.1-
0.3MPa, the pressurized tank of mixed system II maintains constant pressure 0.5-1MPa, preheats as 60-80 DEG C, by control pressure tank valve, will
Mixed system I, mixed system II are with volume ratio 1:3-5 flow at high speed is mixed, and rapidly by narrow flow road, in narrow flow road continuous stream
During dynamic, pre-activate phospholipase D system produces efficient catalytic effect, and serine and soybean lecithin continuously are catalyzed and synthesized into phosphatide
Acyl serine；
（5）Phosphatidylserine crude product is continuously collected in narrow flow road rear end, organic phase is washed with water, vacuum sloughs organic solvent,
Absolute ethyl alcohol insoluble matter is further isolated with absolute ethyl alcohol, vacuum drying obtains phosphatidylserine.
2. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（1）The specific surface area of the porous material is more than 700m2/ g, pore radius is 1-3nm, is preferably made at phospholipase D
In optimal activation state, so as to realize quick catalysis synthetic phospholipid acyl serine.
3. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（2）The serine is Serine, and concentration is 0.3-0.35g/ml.
4. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（3）The content of the phosphorus level in soya lecithin phosphatidylcholine is 5 ~ 35%.
5. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（3）The organic solvent is at least one of cyclohexanone, petroleum ether, acetone, ethyl acetate etc..
6. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（3）The narrow flow road is internal diameter 3-5mm, long 2-3m stainless steel pipes.
7. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（3）The cross section in the narrow flow road is cylindrical shape or triangle or cylindrical shape setting alternate with triangle.
8. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（3）Described narrow flow road periphery sets water-cooling system, and narrow flow channel temp is maintained into 60-80 DEG C.
9. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（4）, flow velocity of the fluid in narrow flow road is 0.5 ~ 1 m/s.
10. a kind of method of continuous high-efficient enzymatic conversion fabricated food phosphatidylserine, its feature according to claim 1
It is：Step（3）In, emulsified using glycerin monostearate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020133126A1 (en) * | 2018-12-27 | 2020-07-02 | 南通励成生物工程有限公司 | Method for preparing phosphatidylserine by ultrasonic assisted enzymolysis |
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| CN1897919A (en) * | 2003-09-25 | 2007-01-17 | 酶学技术有限公司 | Stabilized formulations of phosphatidylserine |
| CN102524402A (en) * | 2011-12-23 | 2012-07-04 | 内蒙古伊利实业集团股份有限公司 | Liquid milk product containing phosphatidylserine |
| CN103131737A (en) * | 2011-11-26 | 2013-06-05 | 江南大学 | Method of preparing phosphatidylserine in enzymic mode |
| CN103966277A (en) * | 2014-05-22 | 2014-08-06 | 南京工业大学 | Method for preparing phosphatidylserine under catalysis of immobilized phospholipase D |
| CN106755154A (en) * | 2017-01-13 | 2017-05-31 | 理星（天津）生物科技有限公司 | A kind of method that use micro passage reaction prepares phosphatidylserine |
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2017
- 2017-06-19 CN CN201710462045.4A patent/CN107034248A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1897919A (en) * | 2003-09-25 | 2007-01-17 | 酶学技术有限公司 | Stabilized formulations of phosphatidylserine |
| CN103131737A (en) * | 2011-11-26 | 2013-06-05 | 江南大学 | Method of preparing phosphatidylserine in enzymic mode |
| CN102524402A (en) * | 2011-12-23 | 2012-07-04 | 内蒙古伊利实业集团股份有限公司 | Liquid milk product containing phosphatidylserine |
| CN103966277A (en) * | 2014-05-22 | 2014-08-06 | 南京工业大学 | Method for preparing phosphatidylserine under catalysis of immobilized phospholipase D |
| CN106755154A (en) * | 2017-01-13 | 2017-05-31 | 理星（天津）生物科技有限公司 | A kind of method that use micro passage reaction prepares phosphatidylserine |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2020133126A1 (en) * | 2018-12-27 | 2020-07-02 | 南通励成生物工程有限公司 | Method for preparing phosphatidylserine by ultrasonic assisted enzymolysis |
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