CN107028769A - A kind of binding agent and its application for being used to simulate the pathologic oral environment of asialia - Google Patents
A kind of binding agent and its application for being used to simulate the pathologic oral environment of asialia Download PDFInfo
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- CN107028769A CN107028769A CN201710260989.3A CN201710260989A CN107028769A CN 107028769 A CN107028769 A CN 107028769A CN 201710260989 A CN201710260989 A CN 201710260989A CN 107028769 A CN107028769 A CN 107028769A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K6/00—Preparations for dentistry
- A61K6/80—Preparations for artificial teeth, for filling teeth or for capping teeth
- A61K6/831—Preparations for artificial teeth, for filling teeth or for capping teeth comprising non-metallic elements or compounds thereof, e.g. carbon
- A61K6/838—Phosphorus compounds, e.g. apatite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K6/00—Preparations for dentistry
- A61K6/20—Protective coatings for natural or artificial teeth, e.g. sealings, dye coatings or varnish
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K6/00—Preparations for dentistry
- A61K6/50—Preparations specially adapted for dental root treatment
- A61K6/54—Filling; Sealing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K6/00—Preparations for dentistry
- A61K6/80—Preparations for artificial teeth, for filling teeth or for capping teeth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K6/00—Preparations for dentistry
- A61K6/80—Preparations for artificial teeth, for filling teeth or for capping teeth
- A61K6/884—Preparations for artificial teeth, for filling teeth or for capping teeth comprising natural or synthetic resins
- A61K6/891—Compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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Abstract
The invention discloses it is a kind of be used for simulate asialia pathologic oral environment binding agent and its application, simulation asialia pathologic oral environment be by lactic acid add NaCl solution in solution ph be 4；Its component of the binding agent is as follows by mass percentage：Unformed calcium phosphate nano particle 40% and PEHB 60%；Its component of wherein PEHB is as follows by weight percentage：The glycerol dimethacrylate 42% of benzene four, ethoxylated bisphenol A dimethylacrylate 42%, phenyl is double（2,4,6 trimethylbenzoyls）Phosphorous oxide 1%, hydroxyethyl methacrylate 10%, bisphenol-A GMA 5%；The present invention can be played simultaneously to be bonded protection and suppresses dental caries progress, and can effectively realize the remineralization of demineralization dentine；Calcium, phosphonium ion can be discharged, local sour environment is neutralized, the remineralization under the sour environment for lacking calcium phosphorus can be realized；Can clinical practice the preventing and treating of patient's rampant caries is reduced in the elderly's cementum caries and extreme saliva.
Description
Technical field
The present invention relates to dentistry Material Field, and in particular to a kind of pathologic oral cavity ring for being used to simulate asialia
The binding agent in border and its application.
Background technology
Saliva plays extremely important role during dental caries progress is suppressed；For healthy population, saliva
Film is covered in the compositions such as the phosphate contained in Tooth surface, saliva, bicarbonate and assigns its stronger buffer capacity all the time；
And saliva is endlessly flowed by the process of swallowing while part swill and bacterium is taken away, moreover it is possible to penetrate into biomembrane
Neutralize the local production acid of cariogenic bacteria；In addition, saliva neutralizes the calcium containing high concentration, phosphorus particle, hydroxyapatite can be both reduced
Solubility, reduces demineralization, can promote remineralization process again.
And asialia/reduction patient then completely or partially loses such protection mechanism, the elderly's salivary secretion work(
It can decline, the incidence of the incidence of cementum caries and extreme saliva reduction patient's rampant caries is all remarkably higher than ordinary person；In calcium, phosphorus
Particle deposit greatly reduces, acid neutralization capacity it is limited in the case of, remineralization process is obstructed, and the preventing and treating of such dental caries does not still have
It is well solved；In addition, material most widely used in the reparation of defect of teeth is compound resin, due to dentine-tree
There is one of secondary principal element for decaying influence repairing effect at a specified future date caused by Micro blazed-grating in fat adhesive failure；Dentine-tree
Fat bonding depends on bonding monomer and penetrates into the contamination layer formed in the collagenous fibres of demineralization, and staiing the stability of layer will directly affect
It is bonded the life-span；In oral environment, collagenous fiber network can by bacterium, enzyme etc. destruction so that cause stain layer denaturation, bonding
Failure；The exposed remineralization for staiing layer effectively reinforced resin-dentine can not only be bonded, and also the site that occur dental caries can be played
Repair, so as to play a part of extension compound resin-Dentin adhensive life-span, suppress secondary caries generation；And clinically also
The material that dental caries enter abduction is protected and suppresses without bonding can be played simultaneously.
The content of the invention
The present invention provides a kind of disease for being used to simulate asialia that can simultaneously play and be bonded protection and suppress dental caries progress
The binding agent of rationality oral environment and its application.
The technical solution adopted by the present invention is：A kind of binding agent for being used to simulate the pathologic oral environment of asialia,
Simulation asialia pathologic oral environment be by lactic acid add NaCl solution in solution ph be 4；
Its component of the binding agent is as follows by mass percentage：Unformed calcium phosphate nano particle 40% and PEHB 60%；
Its component of wherein PEHB is as follows by weight percentage：The glycerol dimethacrylate 42% of benzene four, ethoxylated bisphenol A dimethyl
Acrylate 42%, double (2,4, the 6- trimethylbenzoyl) phosphorous oxides 1% of phenyl, hydroxyethyl methacrylate 10%, bisphenol-A
GMA 5%.
A kind of preparation method for being used to simulate the binding agent of the pathologic oral environment of asialia, it is characterised in that bag
Include following steps：
A, by the glycerol dimethacrylate of benzene four and ethoxylated bisphenol A dimethylacrylate according to mass ratio it is 1:
1 ratio mixing, obtains mixture；
B, double (2,4,6- trimethylbenzoyl) phosphorous oxides of addition phenyl in the mixture that step A is obtained；
C, addition hydroxyethyl methacrylate and bisphenol-A GMA in step B obtains mixture
Obtain PEHB；
D, by PEHB and amorphous phosphoric acid nano-particle it is 3 in mass ratio:2 ratio is mixed to get required binding agent.
A kind of application for being used to simulate the binding agent of the pathologic oral environment of asialia, with the polyamides that end group is amino
Amine-amine type dendrimer solution is used in combination；The end that it is 1mg/mL with concentration by demineralization dentine sample that application method, which is,
After base is the polyamide-amine type branch-shape polymer solution immersion treatment 1h of amino；It is pre- in the coating of demineralization dentine sample surface
The demineralization dentine sample surface of compound resin after treatment is bonded using the binding agent after inorganic agent.
Further, its component of the pretreating agent is as follows by mass percentage：The glycerol dimethacrylate of benzene four
38.4%, hydroxyethyl methacrylate 11.6%, acetone 50%.
Further, release calcium, phosphonium ion in the pathologic oral environment of the simulation asialia.
Further, it is described simulation asialia pathologic oral environment in cushioning liquid pH value.
Further, demineralization dentine sample ore deposit again is promoted in the pathologic oral environment of the simulation asialia
Change.
Further, demineralization dentine sample hardness is promoted in the pathologic oral environment of the simulation asialia
Recover.
The beneficial effects of the invention are as follows：
(1) material of the invention can be played simultaneously bonds protection and suppresses dental caries progress, and can effectively realize demineralization tooth sheet
The remineralization of matter；
(2) amorphous calcium phosphate nano-particle volume in material of the present invention is smaller to be easy to wrap bonding agent into tooth sheet
The tubule formation resin of matter is dashed forward, and ensure that good adhesive effect；
(3) material of the present invention can discharge calcium, phosphonium ion, neutralize local sour environment, can realize the acid for lacking calcium phosphorus
Remineralization under property environment；
(4) present invention can clinical practice the preventing and treating of patient's rampant caries is reduced in the elderly's cementum caries and extreme saliva.
Brief description of the drawings
Fig. 1 is the different dentine shear bond strengths of NACP with PEHB mixed proportions；Wherein A is dentine sample in distillation
Dentine shear bond strength after being soaked 1 day in water, it is viscous that B soaks dentine shearing after 14 days for dentine sample in distilled water
Connect intensity.
Fig. 2 soaks in the solution that different time is measured for four treatment group samples in embodiment 1 in simulating oral cavity environment
Calcium and phosphorus particle concentration；Wherein A is calcium ion concentration in solution；B is phosphate ion concentration in solution.
Fig. 3 soaks lactic acid solution after different time for four treatment group samples in embodiment 2 in simulating oral cavity environment
PH value；Wherein A is the pH value of solution when sample soaks 15min in lactic acid solution；B is that sample soaks in lactic acid solution
The pH value of solution during 60min；C is the pH value of solution when sample soaks 3h in lactic acid solution；D is that sample soaks in lactic acid solution
The pH value of solution when steeping 24h.
Fig. 4 is that four treatment group samples handle after 14 days sample perpendicular to tooth sheet in simulating oral cavity environment in embodiment 3
The scanning electron microscope (SEM) photograph of matter tubule major axis；Wherein A and C is control group scanning electron microscope (SEM) photograph；B and D is PAMAM treatment group ESEMs
Figure；E and G is NACP adhesive treatment groups；F and H is PAMAM+NACP adhesive treatment groups.
Fig. 5 is that four treatment group samples handle after 14 days sample parallel to tooth sheet in simulating oral cavity environment in embodiment 3
The scanning electron microscope (SEM) photograph of matter tubule major axis；Wherein A and C is control group scanning electron microscope (SEM) photograph；B and D is PAMAM treatment group ESEMs
Figure；E and G is NACP adhesive treatment groups；F and H is PAMAM+NACP adhesive treatment groups.
Fig. 6 handles sample after 14 days for four treatment group samples in embodiment 3 in simulating oral cavity environment and measured by EDS
Calcium phosphorus atoms percentage.
Fig. 7 handles the change of the dentine hardness after 14 days, 28 days for four treatment groups in embodiment 4 in simulating oral cavity environment
Change design sketch.
Embodiment
The present invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
A kind of binding agent for being used to simulate the pathologic oral environment of asialia,
Simulation asialia pathologic oral environment be by lactic acid add NaCl solution in solution ph be 4；This is molten
Liquid is the acid cariogenic environment that simulation is laid in without calcium and phosphorus ionses；
Its component of the binding agent is as follows by weight percentage：NACP (unformed calcium phosphate nano particle) 40% He
PEHB60%；Its component of wherein PEHB is as follows by weight percentage：PMGDM (glycerol dimethacrylate of benzene four) 42%,
EBPADMA (ethoxylated bisphenol A dimethylacrylate) 42%, double (2,4, the 6- trimethylbenzoyl) phosphorous oxides 1% of phenyl,
HEMA (hydroxyethyl methacrylate) 10%, BisGMA (bisphenol-A GMA) 5%.
A kind of preparation method for being used to simulate the binding agent of the pathologic oral environment of asialia, it is characterised in that bag
Include following steps：
A, by the glycerol dimethacrylate of benzene four and ethoxylated bisphenol A dimethylacrylate according to mass ratio it is 1:
1 ratio mixing, obtains mixture；
B, double (2,4,6- trimethylbenzoyl) phosphorous oxides of addition phenyl in the mixture that step A is obtained；
C, addition hydroxyethyl methacrylate and bisphenol-A GMA in step B obtains mixture
Obtain PEHB；
D, by PEHB and amorphous phosphoric acid nano-particle it is 3 in mass ratio:2 ratio is mixed to get required binding agent.
A kind of application for being used to simulate the binding agent of the pathologic oral environment of asialia, with the polyamides that end group is amino
Amine-amine type dendrimer solution is used in combination；The end that it is 1mg/mL with concentration by demineralization dentine sample that application method, which is,
After base is the polyamide-amine type branch-shape polymer solution immersion treatment 1h of amino；It is pre- in the coating of demineralization dentine sample surface
The demineralization dentine sample surface of compound resin after treatment is bonded using the binding agent after inorganic agent；End group is the poly- of carboxyl
Amide-amine type dendritic uses third generation end group for the polyamide-amine type branch-shape polymer of carboxyl, i.e. G3-
PAMAM-COOH, purchased from Weihai Chen Yuan new chemical materialses Co., Ltd, abbreviation PAMAM；By 100mgPAMAM powder under normal temperature and pressure
End is dissolved in the PAMAM solution that 10mg/mL is obtained in 10mL distilled water；Amorphous calcium phosphate used is obtained by spray drying process
Nano-particle, specific method is as follows：Calcium carbonate and Dicalcium Phosphate are dissolved in final concentration of to calcium ion in acetum respectively
The final concentration of 5.333mmol/L of 8mmol/L, phosphonium ion, resulting solution, which is sprayed into a heater, evaporates water and acid, electrostatic
Settling vessel collects dried particle, obtains the amorphous calcium phosphate nano-particle that mean particle diameter is 116nm；
Its component of pretreating agent is as follows by mass percentage：The glycerol dimethacrylate 38.4% of benzene four, methyl-prop
Olefin(e) acid hydroxyl ethyl ester 11.6%, acetone 50%；The specific preparation method of pretreating agent is as follows：Pretreating agent is by PMGDM (benzene 4 the third three
Alcohol dimethylacrylate) and 2-HEMA (hydroxyethyl methacrylate) in mass ratio 3.3：1 mixing, then adds acetone molten
Agent makes the mass fraction of acetone after mixing be 50%；The specific preparation method of bonding agent is as follows：PMGDM (the glycerine diformazans of benzene four
Base acrylate) in EBPADMA (ethoxylated bisphenol A dimethylacrylate) in mass ratio 1:It is double that 1% phenyl is added after 1 mixing
(2,4,6- trimethylbenzoyl) phosphorous oxide assigns its photocuring attribute, then adds 10%HEMA (hydroxyethyl methacrylate second
Ester) and 5%BisGMA (bisphenol-A GMA), now gained bonding agent claim PEHB；NACP is (unformed
Calcium phosphate nano particle) and PEHB in mass ratio 2:3 mixing, obtain NACP bonding agents.
Further, release calcium, phosphonium ion in the pathologic oral environment of the simulation asialia.
Further, it is described simulation asialia pathologic oral environment in cushioning liquid pH value.
Further, demineralization dentine sample ore deposit again is promoted in the pathologic oral environment of the simulation asialia
Change.
Further, demineralization dentine sample hardness is promoted in the pathologic oral environment of the simulation asialia
Recover.
Experiment is carried out in simulating oral cavity environment in following embodiments, and demineralization dentine sample is soaked in into 1ml and newly matched somebody with somebody
In the lactic acid solution of system, a lactic acid solution often was changed by 24 hours, heating water bath continues 14 days；This process simulation saliva lacks
Weary patient is in without calcium and phosphorus ionses deposit, the long-term dentine in acid pathologic oral environment.
Demineralization dentine sample preparation methods are as follows in embodiment：
(1) what Outpatient Service Stomatology collection was pulled out grinds one's teeth in sleep without dental caries people, with 0.005% thymol solution disinfection 4h, with distillation
Water soaks, in 4 DEG C of preservations；
(2) 4 × 4 × 1mm is cut to obtain perpendicular to the direction of long axis of tooth at the about 4mm of enamelo-cemental junction top with diamond blade
Dentine sample；
3. dentine sample is with 37% phosphoric acid solution acid etching 15s；
(4) the ultrasonically treated 10min of dentine sample distilled water of demineralization is to remove the soft dirt of surface bacterial plaque, with PBS (phosphoric acid
Buffered saline, pH=7.4) solution immersion, in 4 DEG C of preservations.
Amorphous calcium phosphate nano-particle and PEHB mixed proportion first in screening binding agent, specific method are as follows：
Dentine sample coating pretreating agent after demineralization, handles 15s, air gun featheriness evaporates solvent；Coating adhesive,
Photocuring 10s；Then the stainless steel substrates that there is circular windowing (diameter 4mm, thick 1.5mm) in center are placed in and are coated with bonding agent
Above dentine sample, compound resin (TPH, purchased from Deng Shibai companies), photocuring 1min are filled up at windowing；After the completion of bonding
Dentine sample soaks 1 day/14 days for 37 DEG C in distilled water, then tests dentine shear bond strength SD；Test result is such as
Shown in Fig. 1：Amorphous calcium phosphate nano-particle adding proportion is from 10%~40%, and this shear bond strength of tooth is poor without statistics
It is different；Water aging 14 days is with the dentine shear bond strength of 1 day without significant difference；Choose respectively and put into Clinical practice at present
Total etching/Self-etching bond agent is used as positive control；It is one pack system total etching adhesive system Prime＆Bond NT to 1 in figure
(Dentsply,Milford,DE)；To 2 be one pack system self-etching adhesives system Scotchbond Multi-Purpose (SBMP,
3M,St.Paul,MN)。
Demineralization dentine sample is divided into four groups in following embodiments, every group of six samples, its specific processing method is as follows：
(1) control group：Each demineralization dentine sample is dried up after being handled with 100 μ L distilled water immersions.
(2) PAMAM groups：Each demineralization dentine sample soaks 1h in 100 μ L PAMAM solution, is cleaned with distilled water.
(3) NACP groups：NACP bonding agents are placed in 2 × 2 × 12mm rectangular die, consolidated per sidelight according to 1min
NACP strip of glue after change；Each demineralization dentin surface contacts with three NACP strip of glue simultaneously.
(4) PAMAM+NACP groups：Each demineralization dentine sample is with after 100 μ LPAMAM solution immersion treatments 1h, and surface connects
Touch the NACP resin streaks for placing 2 × 2 × 12mm after 3 pieces of photocurings (with NACP groups).
Embodiment 1
Tested using above-mentioned simulating oral cavity environment, respectively at the 1st, determine the breast for being soaked with sample within 3,5,7,10 and 14 days
Calcium and phosphorus ionses concentration in acid solution；1mL solution Spectrophotometric Determination of Calcium phosphate ion concentration is gathered every time, while supplement etc.
Measure new lactic acid solution.
Calcium and phosphorus ionses concentration measurement is as shown in Fig. 2 it can be seen that calcium in PAMAM groups and control group solution
Phosphate ion concentration is almost 0；NACP groups and PAMAM+NACP groups have the calcium and phosphorus ionses release from NACP binding agents, due to breast
The renewal of acid solution, is in reduction trend from the 1st day to the concentration of the 14th day calcium and phosphorus ionses；In the 14th day NACP groups and PAMAM+
The calcium and phosphorus ionses concentration of NACP groups remains above PAMAM groups and control group；Illustrate that NACP binding agents can be in the acidity laid in without calcium phosphorus
Sustained release calcium and phosphorus ionses in environment.
Embodiment 2
Tested using above-mentioned simulating oral cavity environment, respectively at the 1st, determine the breast for being soaked with sample within 3,5,7,10 and 14 days
The pH of acid solution (initial pH is 4)；Sample is soaked in lactic acid solution for daily 24 hours, and a lactic acid solution is changed per 24h；
PH electrodes monitor the pH of lactic acid solution in real time.
Test result is as shown in figure 3, PAMAM groups and the pH value of control group-lactic acid solution maintain essentially in 4；NACP groups and
First day lactic acid solution of PAMAM+NACP groups rises to 6.9 when rising to 6.5,3h when rising to 6.1,60min when 15min；Tieed up during 24h
Hold 6.9；Due to the renewal of lactic acid solution, pH value is in slightly decrease trend with time stepping method；Lactic acid solution is when 15min within 14th day
6.5 are risen to when rising to 5.5,3h；The pH value of NACP groups and PAMAM+NACP groups be far above without NACP binding agents PAMAM groups with
Control group；Illustrate that NACP binding agents have stronger acid neutralization capacity, it is possible to increase pH, improve cariogenic sour environment.
Embodiment 3
Tested using above-mentioned simulating oral cavity environment, after demineralization dentine sample soaks 14 days, each sample Buddha's warrior attendant
Stone blade is cut in half along center line, and half is used to detect cross section (perpendicular to dentinal tubule direction), and second half is used to detect
Vertical section (parallel to the direction of dentinal tubule)；Every part of sample is soaked in the PBS solution containing 1% glutaraldehyde in 4 DEG C of preservations
4h, is then dehydrated with graded ethanol；Metal spraying after 100% hmds is rinsed；Scanning electron microscopic observation sample is used, while with
Energy dispersive spectrometry detects chemical element component, and its result is as Figure 4-Figure 6.
Perpendicular to dentinal tubule scanning direction Electronic Speculum detection figure as shown in figure 4, from Fig. 4 A and Fig. 4 C, Fig. 4 B and Fig. 4 D
It can be seen that all dentinal tubules are in open state, and no mineral deposit, higher image graph 4C and Fig. 4 D of multiplication factor can
See exposed collagenous fibres；It can be seen that NACP groups realize a certain degree of remineralization, dentine table from Fig. 4 E and Fig. 4 G
There is a small amount of mineral deposit in face and dentinal tubule；Dentine in PAMAM+NACP groups is can be seen that from Fig. 4 F and Fig. 4 H
It can be seen that more neogenic mineral matter.
ESEM detection image parallel to dentinal tubule direction is as shown in figure 5, its detection range is in top layer tooth sheet
Within matter in the range of 2~30 μm；As can be seen that dentine in Fig. 5 A and Fig. 5 C control groups and Fig. 5 B and Fig. 5 D PAMAM groups
Mineral deposit, the collagenous fibres of the visible exposure of the higher image of multiplication factor are had no in tubule；Can be with from Fig. 5 E and Fig. 5 G
Find out in NACP group dentinal tubules there is a small amount of mineral deposit；It can be seen that PAMAM+NACP group tooth sheets from Fig. 5 F and Fig. 5 H
Visible substantial amounts of neogenic mineral crystal in matter tubule.
Atomic percent of the calcium P elements counted according to EDS testing results in dentine is as shown in Figure 6；It can be seen that
Calcium P elements percentage is very low in control group and PAMAM groups, and calcium phosphorus element content is substantially zeroed；Illustrate dentine sample surface
Complete demineralization；NACP groups are it can be seen that compare control group and PAMAM group calcium P elements percentages are obviously improved；PAMAM+
The calcium P elements that NACP groups compare NACP groups are higher；Illustrate PAMAM+NACP group dentine remineralization degree highests, i.e. NACP glues
Knot agent is combined with PAMAM can provide calcium phosphorus deposit in improved sour environment is lacked, and effectively realize remineralization.
Embodiment 4
Tested using above-mentioned simulating oral cavity environment, respectively at the hard of the four groups of dentine samples of measurement in the 14th day and the 28th day
The hardness of dentine is used as control after degree, measurement complete health dentine and acid etching；Shown in test result figure Fig. 7, a tables in figure
Show healthy dentine sample, b represents dentine sample after acid etching, and c represents 14 days dentine samples after acid etching, and d is represented after acid etching
28 days dentine samples；Vickers diamond is popped one's head in and stops 10s with being supported on sample for 20g to measure hardness, and each sample is surveyed
Amount 6 times, averages.
It can be seen from figure 7 that the hardness of complete health dentine sample is 0.55GPa, hardness is down to after acid etching
0.31GPa；Soaked in lactic acid solution after certain time, PAMAM groups and control group dentine sample hardness were down in the 14th day
0.51GPa, 0.05GPa is down at the 28th day；The dentine hardness of NACP groups is higher than PAMAM groups and control group, PAMAM+NACP
The dentine hardness of group rose to 0.47GPa, near 0.56GPa at the 28th day when the 14th day, hard close to complete health dentine
Degree；Illustrate that PAMAM solution is combined with NACP resins in the pathologic oral environment for the asialia that the present invention is simulated to promote
The recovery of demineralization dentine hardness.
The present invention can preferably in-vitro simulated asialia patient without calcium and phosphorus ionses deposit, long-term in acid pathologic mouthful
Chamber environment；And NACP bonding agents can discharge calcium phosphorus particle with PAMAM combinations in this extreme environment, promote demineralization dentine again
Mineralising；The therapeutic scheme of the dental caries patient of saliva reduction/shortage is caused to provide new possibility for a variety of causes；Be conducive to the elderly
The dental caries preventing and treating of group and dental caries Susceptible population and tooth body are preserved；Additionally be expected to be used for improving resin-dentine bonding, reduce after
Send out the generation of dental caries.
Claims (8)
1. a kind of binding agent for being used to simulate the pathologic oral environment of asialia, it is characterised in that
Simulation asialia pathologic oral environment be by lactic acid add NaCl solution in solution ph be 4；
Its component of the binding agent is as follows by mass percentage：Unformed calcium phosphate nano particle 40% and PEHB 60%；Wherein
Its component of PEHB is as follows by weight percentage：The glycerol dimethacrylate 42% of benzene four, ethoxylated bisphenol A dimethyl allene
Acid esters 42%, double (2,4, the 6- trimethylbenzoyl) phosphorous oxides 1% of phenyl, hydroxyethyl methacrylate 10%, bisphenol-A methyl
Glycidyl acrylate 5%.
2. a kind of preparation method of bonding agent as claimed in claim 1, it is characterised in that comprise the following steps：
A, by the glycerol dimethacrylate of benzene four and ethoxylated bisphenol A dimethylacrylate according to mass ratio it is 1:1
Ratio is mixed, and obtains mixture；
B, double (2,4,6- trimethylbenzoyl) phosphorous oxides of addition phenyl in the mixture that step A is obtained；
C, add hydroxyethyl methacrylate and bisphenol-A GMA in step B obtains mixture and obtain
PEHB；
D, by PEHB and amorphous phosphoric acid nano-particle it is 3 in mass ratio:2 ratio is mixed to get required binding agent.
3. a kind of application of binding agent as claimed in claim 1, it is characterised in that：With the polyamidoamine tree that end group is amino
Dendritic polymer solution is used in combination；Application method is that the end group for being 1mg/mL with concentration by demineralization dentine sample is amino
After polyamide-amine type branch-shape polymer solution immersion treatment 1h；Adopted after demineralization dentine sample surface coating pretreating agent
The demineralization dentine sample surface of compound resin after treatment is bonded with binding agent described in claim 1.
4. application according to claim 3, it is characterised in that：Its component of the pretreating agent is as follows by mass percentage：
The glycerol dimethacrylate 38.4% of benzene four, hydroxyethyl methacrylate 11.6%, acetone 50%.
5. application according to claim 3, it is characterised in that：In the pathologic oral environment of the simulation asialia
Discharge calcium, phosphonium ion.
6. application according to claim 3, it is characterised in that：In the pathologic oral environment of the simulation asialia
The pH value of cushioning liquid.
7. application according to claim 3, it is characterised in that：In the pathologic oral environment of the simulation asialia
Promote demineralization dentine sample remineralization.
8. application according to claim 3, it is characterised in that：In the pathologic oral environment of the simulation asialia
Promote the recovery of demineralization dentine sample hardness.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109298146A (en) * | 2018-09-11 | 2019-02-01 | 郑州大学第附属医院 | Imitate the experimental provision of oral environment and the research method of dental enamel Demineralization |
| CN112999094A (en) * | 2021-03-01 | 2021-06-22 | 广西医科大学附属口腔医院 | Nano complex for inducing bionic internal mineralization of collagen fibers and preparation method thereof |
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| CN105267046A (en) * | 2015-11-02 | 2016-01-27 | 浙江大学 | Method of quickly repairing demineralized dentine |
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| CN105267046A (en) * | 2015-11-02 | 2016-01-27 | 浙江大学 | Method of quickly repairing demineralized dentine |
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| LIANG, KUNNENG ET AL.: "Poly (amido amine) and nano-calcium phosphate bonding agent to remineralize tooth dentin in cyclic artificial saliva/lactic acid", 《MATERIALS SCIENCE AND ENGINEERING C》 * |
| LING ZHANG ET AL.: "Rechargeable dental adhesive with calcium phosphate nanoparticles for long-term ion release", 《J DENT.》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109298146A (en) * | 2018-09-11 | 2019-02-01 | 郑州大学第附属医院 | Imitate the experimental provision of oral environment and the research method of dental enamel Demineralization |
| CN109298146B (en) * | 2018-09-11 | 2021-09-28 | 郑州大学第一附属医院 | Experimental device for simulating oral tooth enamel demineralization and research method for tooth enamel demineralization |
| CN112999094A (en) * | 2021-03-01 | 2021-06-22 | 广西医科大学附属口腔医院 | Nano complex for inducing bionic internal mineralization of collagen fibers and preparation method thereof |
| CN112999094B (en) * | 2021-03-01 | 2022-06-17 | 广西医科大学附属口腔医院 | Nano complex for inducing bionic internal mineralization of collagen fibers and preparation method thereof |
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