CN107024415A - A kind of device for studying molecular migration motion - Google Patents
A kind of device for studying molecular migration motion Download PDFInfo
- Publication number
- CN107024415A CN107024415A CN201710279605.2A CN201710279605A CN107024415A CN 107024415 A CN107024415 A CN 107024415A CN 201710279605 A CN201710279605 A CN 201710279605A CN 107024415 A CN107024415 A CN 107024415A
- Authority
- CN
- China
- Prior art keywords
- laser
- sample
- level crossing
- sample surfaces
- apart
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000033001 locomotion Effects 0.000 title claims abstract description 19
- 238000013508 migration Methods 0.000 title claims abstract description 11
- 230000005012 migration Effects 0.000 title claims abstract description 11
- 230000003287 optical effect Effects 0.000 claims abstract description 21
- 238000006073 displacement reaction Methods 0.000 claims abstract description 11
- 238000009792 diffusion process Methods 0.000 claims description 20
- 239000013307 optical fiber Substances 0.000 claims description 7
- 238000000879 optical micrograph Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 38
- 210000004027 cell Anatomy 0.000 description 16
- 238000000034 method Methods 0.000 description 5
- 239000007844 bleaching agent Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000737 periodic effect Effects 0.000 description 3
- 241000931526 Acer campestre Species 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- 230000005697 Pockels effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000002376 fluorescence recovery after photobleaching Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 239000005090 green fluorescent protein Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N2013/003—Diffusion; diffusivity between liquids
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to optical microphotograph field, a kind of device for studying molecular migration motion, laser, the polarizer, Pockers cell, analyzer, optical splitter, sample is collinear, the laser beam of the laser transmitting is enabled to pass through the polarizer, Pockers cell, two beam laser are divided into by the optical splitter after analyzer, beam of laser is passed through apart from L direct irradiations in sample surfaces, another beam of laser reaches level crossing after apart from a, sample surfaces are irradiated in after level crossing reflects, the above-mentioned two beam laser for being irradiated in the sample surfaces are angle o, the interference figure of light can be produced at sample surfaces, it is described adjustable apart from a and L, so as to change sample surfaces interference fringe spacing i by the value for changing the angle θ；The level crossing is connected with displacement platform and position modulation can be carried out to the level crossing so that the level crossing carries out sinusoidal vibration along its normal orientation with frequency 800Hz, so as to change position of interference fringe at the sample surfaces.
Description
Technical field
The present invention relates to optical microphotograph field, particularly a kind of analysis it is more simple and it is accurate, can measure to spread and be
A kind of device of several research molecular migration motions.
Background technology
Optically powered is also known as photobleaching, refers to fluorescent material is ejected under the irradiation of light fluorescence intensity over time
The phenomenon that passage progressively weakens or even disappeared.The quality of fluorescence imaging is largely dependent upon fluorescence signal intensity, improves and swashs
Luminous intensity can improve signal intensity, but when the intensity of exciting light exceedes certain limit, light absorbs tend to saturation, not
Excited state molecule is reversibly destroyed, here it is photobleaching phenomenon.Fluorescence recovery after photobleaching is to use lipophilicity or hydrophily
Fluorescence molecule, such as fluorescein, green fluorescent protein and albumen or lipid couple, and for detecting, mark molecule is thin in live body
Cellular surface or cell interior motion and its migration rate.The principle of recovery technology after fluorescent bleach is：Shone using superlaser
The a certain specific region of cell is penetrated, makes the fluorescence molecule of the region internal labeling is irreversible to be quenched, this region claims fluorescent bleach
Area.Subsequently, as the motion of the lipid molecular or protein molecule in cytoplasm, surrounding non-bleached area fluorescence molecule is constantly to light
Bleach area migration, the fluorescence intensity in fluorescent bleach area is little by little returned to previous level, on this basis in the urgent need to
It is a kind of that there is the device and method that can be used in measuring diffusion coefficient.
What the method that fluorescence recovers after traditional use photobleaching had to study the technology of the motions such as molecular migration diffusion
Defect has：In order to reach the demand of light signal collection in experiment, the density needs of fluorescence molecule are very high；The measurement of beam profile is needed
It is very accurate；The monitoring of photobleaching process is not readily reachable by, so as to need higher light intensity to carry out photobleaching, is so possible to
The structure of sample molecule can be destroyed, particularly some biological sample molecules.A kind of device for studying molecular migration motion makes
Photobleaching is carried out to sample with the hot spot of plane and subsequent monitoring detection, solve drawbacks described above.
The content of the invention
In order to solve the above problems, apparatus of the present invention cause the facular area that light and dark striated is produced in sample surfaces
Domain, after carrying out photobleaching with strong light in bright fringes region, continues to keep relatively low intensity illumination, to detect pair between light and shade striped
Changed with time than degree, and the information such as the diffusion coefficient of testing molecule are obtained by subsequent analysis, Cluster Brownian diffusion coefficient can
Independently to obtain, it is the average value in whole spot pattern region.
The technical solution adopted in the present invention is：
It is described it is a kind of study molecular migration motion device mainly include laser, the polarizer, Pockers cell, analyzer,
Optical splitter, sample, displacement platform, level crossing, optical fiber, optical filter, photomultiplier, lock-in amplifier, cable, the lock mutually amplify
Device connects the Pockers cell and the displacement platform by cable respectively, the luminous energy of the sample surfaces by the optical fiber and
The optical filter is transferred in the photomultiplier, and the Pockers cell can make laser generation one extremely short less than 1 second
Full intensity pulse, the photomultiplier, which can collect after photobleaching the emerging fluorescence due to molecule diffusion motion, to be believed
Number, then analyzed by the lock-in amplifier, so as to obtain the sample surfaces bright fringes part and dark fringe part
Fluorescence intensity average contrast C (t).
The laser, the polarizer, Pockers cell, analyzer, optical splitter, sample are collinear, enable to the laser
The laser beam of transmitting is divided into two beam laser, beam of laser after the polarizer, Pockers cell, analyzer by the optical splitter
By apart from L direct irradiations, in the sample surfaces, another beam of laser reaches the level crossing after apart from a, by described
The sample surfaces are irradiated in after level crossing reflection, the above-mentioned two beam laser for being irradiated in the sample surfaces are angle o, can be in institute
The interference figure that light is produced at sample surfaces is stated, it is described adjustable apart from a and L, so as to be changed by the value for changing the angle θ
The sample surfaces interference fringe spacing i；The level crossing is connected with the displacement platform and the level crossing can be carried out
Position modulation so that the level crossing carries out sinusoidal vibration along its normal orientation with frequency 800Hz, so as to change the sample table
Position of interference fringe at face.
Use the method and step that apparatus of the present invention are studied for：
The laser beam that laser described in one, is launched is after the polarizer, Pockers cell, analyzer, by the light splitting
Device is divided into two beam laser, and beam of laser is passed through apart from L direct irradiations in the sample surfaces, and another beam of laser is after apart from a
The level crossing is reached, the sample surfaces are irradiated in after level crossing reflection, it is above-mentioned to be irradiated in the sample surfaces
Two beam laser it is angle o so that at the sample surfaces produce light interference figure；
Two, can change the value of the angle θ by adjusting the length apart from a and L, so as to change the sample table
Face interference fringe spacing i；
Three, make the pulse of the laser one extremely short full intensity less than 1 second of generation by the Pockers cell, to bright fringes
The molecule of the corresponding species of the fluorescence labeling at place carries out optically powered, and after optically powered process terminates, the laser is continuously launched
Light intensity is I (r, t) laser to detect the change in fluorescence of the sample surfaces；
Four, carry out position modulation by the displacement platform to the level crossing so that the level crossing is along its normal orientation
Sinusoidal vibration is carried out with frequency 800Hz, so as to change position of interference fringe at the sample surfaces；
Described in five, photomultiplier collect photobleaching after due to molecule diffusion motion emerging fluorescence signal, then
Analyzed by the lock-in amplifier, thus obtain sample surfaces bright fringes part and dark fringe part fluorescence intensity it
Than i.e. average contrast C (t), fluorescence signal F (t) and the concentration for the molecule being fluorescently labeled that the photomultiplier is measured
cm(r, t) and detection light intensity I (r, t) are related
WhereinWithIt is cm(r, t) and I (r, t) spatial Fourier transform, F (t) can be decomposed into
The harmonic progression of modulating frequency, in experiment, the harmonic component f of single order and second order1And f (t)2(t) can mutually it be amplified by the lock
Device is obtained simultaneously；
Experimental data final six, provides the average contrast between dark fringe and bright fringes
Above formula characterizes Blang's dispersal behavior with an exponential damping；
Seven, byRelaxation time τ can be obtainedq, by relaxation time τqWith the pass of diffusion coefficient D
SystemQ is the space periodic of candy strip, can obtain diffusion coefficient D.
The sample surfaces can add a photomask for having circular open, and the measured zone of the sample surfaces is located at institute
State in opening, the material of photomask is to the reflectivity very little of light, can reduce due to the sample surfaces that laser facula is caused
Influence of the veiling glare of non-measured region to experiment signal to noise ratio；Different optical maser wavelength 488nm, 532nm, 633nm can be used
Etc. being tested, and the result that each optical maser wavelength condition experiment of Integrated comparative is obtained, can more accurately reflect molecule
Travel motion；To large biological molecule sample, laser intensity is less than 250W/cm during photobleaching2, and the burst length is less than 400ms.
Pockers cell is a kind of electro-optical device, and it includes an electro-optic crystal passed through by light, based on Pockels effect,
The polarization direction of light can be regulated and controled by the voltage being applied on crystal and change light and pass through the bit phase delay after crystal.
Due to the diffusion of molecule, the contrast C (t) reduces with the time, using same candy strip come to sample
Product carry out optically powered and detection and collect signal ensure that them by same wavelength to characterize.Visited in the lock-in amplifier
The composition of the fundamental frequency of the fluorescence intensity of survey is changed over time, and meets the diffusion equation of single mode, and contrast C (t) is according to simple
Exponential dampingWherein relaxation time τqIt is relevant with diffusion coefficient DQ is candy strip
Space periodic, so, by changing q, can verify that Blang diffusion rule and accurately decision brown-Zsigmondy movements diffusion coefficient.
Constant C0、C1With brown-Zsigmondy movements and not relative fractions between brown-Zsigmondy movementsWithIt is relevant.
In the situation of the complex sample containing N kind different motion populations,It can be extended to
The diffusion coefficient and fraction of kth kind population be respectively
The beneficial effects of the invention are as follows：
Present invention analysis is more simple and accurate, it is easier to detects the small change of diffusion coefficient, or differentiates not of the same race
Difference between group；Carry out optically powered and detection to sample and collect the periodic sinusoidal pattern used in signal can be used for
The diffusion equation of a single mode is detected, the pattern of optically powered can be an increasingly complex figure, usually Gaussian spot；Separately
Outside, apparatus of the present invention can more easily change the parameter of interference fringe, to spread the true of rule in reciprocal space Direct Test
Reality, the device is read and the same pattern of optically powered, can be applied to research, DNA diffusion of the polymer in the diffusion at interface
And the field such as electrophoresis.
Brief description of the drawings
Further illustrated with reference to the figure of the present invention：
Fig. 1 is schematic diagram of the present invention.
In figure, 1. lasers, 2. polarizers, 3. Pockers cells, 4. analyzers, 5. optical splitters, 6. samples, 7. displacement platforms,
8. level crossing, 9. optical fiber, 10. optical filters, 11. photomultipliers, 12. lock-in amplifiers, 13. cables.
Embodiment
If Fig. 1 is schematic diagram of the present invention, a kind of device for studying molecular migration motion mainly includes laser 1, risen
Inclined device 2, Pockers cell 3, analyzer 4, optical splitter 5, sample 6, displacement platform 7, level crossing 8, optical fiber 9, optical filter 10, photomultiplier transit
Pipe 11, lock-in amplifier 12, cable 13, the lock-in amplifier 12 connect the Pockers cell 3 and institute respectively by cable 13
Rheme moving stage 7, the luminous energy on the surface of sample 6 is transferred to the photomultiplier transit by the optical fiber 9 and the optical filter 10
In pipe 11, the Pockers cell 3 can make laser produce the pulse of the extremely short full intensity for being less than 1 second, and the photoelectricity is again
The emerging fluorescence signal due to molecule diffusion motion can be collected after photobleaching by increasing pipe 11, then pass through the lock-in amplifier
12 are analyzed, so as to obtain the surface bright fringes part of sample 6 and the average contrast C of the fluorescence intensity of dark fringe part
(t)。
The laser 1, the polarizer 2, Pockers cell 3, analyzer 4, optical splitter 5, sample 6 is collinear, enables to described
The laser beam that laser 1 is launched is that two beams swash by 5 points of the optical splitter after the polarizer 2, Pockers cell 3, analyzer 4
Light, beam of laser is passed through apart from L direct irradiations in the surface of sample 6, and another beam of laser reaches the plane after apart from a
Mirror 8, is irradiated in the surface of sample 6 after the level crossing 8 reflection, and above-mentioned two beams for being irradiated in the surface of sample 6 swash
Light is angle o, and the interference figure of light can be produced at the surface of sample 6, described adjustable apart from a and L, so as to by changing
The value for becoming the angle θ changes the surface fringe spacing i of sample 6；Be connected with the displacement platform 7 level crossing 8 and
Position modulation can be carried out to the level crossing 8 so that the level crossing 8 carries out sinusoidal along its normal orientation with frequency 800Hz
Vibration, so as to change position of interference fringe at the surface of sample 6.
Claims (1)
1. a kind of device for studying molecular migration motion, mainly including laser (1), the polarizer (2), Pockers cell (3), analyzing
Device (4), optical splitter (5), sample (6), displacement platform (7), level crossing (8), optical fiber (9), optical filter (10), photomultiplier
(11), lock-in amplifier (12), cable (13), the lock-in amplifier (12) connect the Pu Keer respectively by cable (13)
Box (3) and the displacement platform (7), the luminous energy on sample (6) surface are passed by the optical fiber (9) and the optical filter (10)
Defeated to arrive in the photomultiplier (11), the Pockers cell (3) can make laser produce one and be less than 1 second extremely short full strong
The pulse of degree, the photomultiplier (11) can collect after photobleaching the emerging fluorescence signal due to molecule diffusion motion,
Then analyzed by the lock-in amplifier (12), so as to obtain the sample (6) surface bright fringes part and dark fringe
The average contrast C (t) of partial fluorescence intensity,
It is characterized in that：The laser (1), the polarizer (2), Pockers cell (3), analyzer (4), optical splitter (5), sample (6)
Collinear, the laser beam for enabling to the laser (1) to launch passes through the polarizer (2), Pockers cell (3), analyzer (4)
Two beam laser are divided into by the optical splitter (5) afterwards, beam of laser is passed through apart from L direct irradiations in the sample (6) surface, another
Shu Jiguang reaches the level crossing (8) after apart from a, and the sample (6) table is irradiated in after the level crossing (8) reflection
Face, the above-mentioned two beam laser for being irradiated in the sample (6) surface are angle o, and the dry of light can be produced at the sample (6) surface
Pattern is related to, it is described adjustable apart from a and L, so as to change sample (6) the Surface Interference bar by the value for changing the angle θ
Line spacing i；The level crossing (8) is connected with the displacement platform (7) and position modulation can be carried out to the level crossing (8),
So that the level crossing (8) carries out sinusoidal vibration along its normal orientation with frequency 800Hz, so as to change the sample (6) surface
Locate position of interference fringe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710279605.2A CN107024415A (en) | 2017-04-17 | 2017-04-17 | A kind of device for studying molecular migration motion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710279605.2A CN107024415A (en) | 2017-04-17 | 2017-04-17 | A kind of device for studying molecular migration motion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107024415A true CN107024415A (en) | 2017-08-08 |
Family
ID=59527099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710279605.2A Withdrawn CN107024415A (en) | 2017-04-17 | 2017-04-17 | A kind of device for studying molecular migration motion |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107024415A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672559A (en) * | 2019-10-25 | 2020-01-10 | 西安交通大学 | Device and method for simultaneously measuring binary system thermal diffusivity and mutual diffusivity |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000031518A1 (en) * | 1998-11-24 | 2000-06-02 | Cambridge Research & Instrumentation, Inc. | Fluorescence polarization assay system and method |
| CN103091297A (en) * | 2013-01-30 | 2013-05-08 | 浙江大学 | Super-resolution microscope method and device based on random fluorescence bleaching |
| CN104062750A (en) * | 2014-06-18 | 2014-09-24 | 浙江大学 | Method and device for two-photon fluorescence stimulated emission differential super-resolution microscopy |
| CN106124468A (en) * | 2016-06-20 | 2016-11-16 | 浙江大学 | A kind of based on photoactivation and the super-resolution fluorescence microscopy method of Structured Illumination and device |
| CN206618657U (en) * | 2017-04-17 | 2017-11-07 | 金华职业技术学院 | A kind of device for studying molecular migration motion |
-
2017
- 2017-04-17 CN CN201710279605.2A patent/CN107024415A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000031518A1 (en) * | 1998-11-24 | 2000-06-02 | Cambridge Research & Instrumentation, Inc. | Fluorescence polarization assay system and method |
| CN103091297A (en) * | 2013-01-30 | 2013-05-08 | 浙江大学 | Super-resolution microscope method and device based on random fluorescence bleaching |
| CN104062750A (en) * | 2014-06-18 | 2014-09-24 | 浙江大学 | Method and device for two-photon fluorescence stimulated emission differential super-resolution microscopy |
| CN106124468A (en) * | 2016-06-20 | 2016-11-16 | 浙江大学 | A kind of based on photoactivation and the super-resolution fluorescence microscopy method of Structured Illumination and device |
| CN206618657U (en) * | 2017-04-17 | 2017-11-07 | 金华职业技术学院 | A kind of device for studying molecular migration motion |
Non-Patent Citations (1)
| Title |
|---|
| LAURENT JOURDAINNE 等: "Dynamics of Poly(L-lysine) in Hyaluronic Acid/Poly(L-lysine) Multilayer Films Studied by Fluorescence Recovery after Pattern Photobleaching", LANGMUIR, vol. 24, no. 5, pages 7842 - 7847 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672559A (en) * | 2019-10-25 | 2020-01-10 | 西安交通大学 | Device and method for simultaneously measuring binary system thermal diffusivity and mutual diffusivity |
| CN110672559B (en) * | 2019-10-25 | 2021-01-19 | 西安交通大学 | Device and method for simultaneously measuring binary system thermal diffusivity and mutual diffusivity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5215883A (en) | Electrophoretic mobility of fluorophore labeled particles in gels by fluorophore movement after photobleaching | |
| Dertinger et al. | Two‐focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements | |
| CN109444053B (en) | Transient heat transfer microscope and method for measuring micro-area heat by using same | |
| CN103210302B (en) | Optical analysis device for observing polarisation characteristics of single light-emitting particle, optical analysis method and optical analysis computer program therefor | |
| CN104359892A (en) | Different modal molecular vibration spectrum detection and imaging device and method | |
| KR100929202B1 (en) | Image acquisition device and method using coherent anti-stokes Raman scattering | |
| CN107121362A (en) | A kind of method for studying molecular migration motion | |
| Hansen et al. | Fluorescence correlation spectroscopy with patterned photoexcitation for measuring solution diffusion coefficients of robust fluorophores | |
| CN104508462A (en) | Spectral analysis device using confocal microscope or multiphoton microscope optical system, spectral analysis method, and spectral analysis computer program | |
| US9518928B2 (en) | Optode for determining chemical parameters | |
| CN206618657U (en) | A kind of device for studying molecular migration motion | |
| CN107024415A (en) | A kind of device for studying molecular migration motion | |
| Palazzo et al. | Diffusion measuring techniques | |
| KR101758114B1 (en) | Method for measuring fluorescence lifetime according to energy transfer | |
| Ide et al. | Fluorescence lifetime resolution with phase fluorometry | |
| Schwille et al. | Principles and applications of fluorescence correlation spectroscopy (FCS) | |
| Champion et al. | Comparison between two methods to measure translational diffusion of a small molecule at subzero temperature | |
| Madden et al. | Mining the polarization-dependence of nonlinear optical measurements | |
| Didier et al. | Two-photon two-focus fluorescence correlation spectroscopy with a tunable distance between the excitation volumes | |
| Corcoran | Laser-induced fluorescence spectroscopy (LIF) | |
| CN207894818U (en) | A kind of water quality detecting device | |
| JPH03154850A (en) | Specimen inspecting device | |
| JP5751563B2 (en) | Frequency domain fluorescence measurement system | |
| Premadasa et al. | Spatially co-registered wide-field nonlinear optical imaging of living and complex biosystems in a total internal reflection geometry | |
| RU2563318C1 (en) | Method of optical determining and identification in liquids of micro-objects containing dna and device for its implementation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170808 |
|
| WW01 | Invention patent application withdrawn after publication |