CN107022579A - The method that xylitol is prepared using bamboo shoot process discarded object - Google Patents
The method that xylitol is prepared using bamboo shoot process discarded object Download PDFInfo
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- CN107022579A CN107022579A CN201710317518.1A CN201710317518A CN107022579A CN 107022579 A CN107022579 A CN 107022579A CN 201710317518 A CN201710317518 A CN 201710317518A CN 107022579 A CN107022579 A CN 107022579A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
Abstract
The invention discloses a kind of from the method that xylitol is prepared using bamboo shoot process discarded object, by Compositions of Bamboo Shoot Shell by hydrolysis, neutralization, decolouring, ion exchange, fermentation step, xylitol has been obtained.The present invention produces xylitol using biotransformation method, repeatedly purification, purification step can be saved, xylose utilization rate and xylitol conversion rate is improved, realizes that the key technology of xylitol low cost breaks through, increase farmers' income, improve the international occupancy volume of China's xylitol and competitiveness.
Description
Technical field
The present invention relates to a kind of preparation method of xylitol, and in particular to one kind is discarded using agricultural wastes bamboo shoot process
The method of thing clean manufacturing xylitol.
Background technology
Xylitol is a kind of natural sweetener, and chemical entitled " penta pentasaccharides ", is a kind of pentose.Appearance and the sense of taste all with sugarcane
Sugar is similar, and other sugar alcohols compare, and are the sweeteners of most sweet tea in polyalcohol.Be widely used to food, medicine, printing and dyeing, weaving,
The fields such as national defence, leather and chemical industry.In the 1970s, xylitol is special by international grain farmer and health organization food additive regulations
The committee of family (JECFA) is approved as A based food additives, in June, 1999 Codex Alimentary Commission (CAC) approval xylitol
For " in food can by normally produce the food additives needed to use ".Recently as domestic and foreign scholars to its physiology work(
The research of energy characteristic, it was demonstrated that xylitol has different physiological roles, is a kind of important functional Sugar Alcohol.
Xylitol is the intermediate of human body carbohydate metabolism, lack in vivo insulin influence glycometabolism in the case of, without pancreas
Island element promotes, and just can pass through cell membrane, be absorbed by tissue utilization, for cell with nutrition and energy, and will not cause blood glucose value liter
Height, eliminates the symptom more than three after diabetes patient takes (many foods, many drinks, diuresis), is the nutrition for being best suitable for patients with diabetes mellitus
The sugar substitutes of property；Xylitol can promote liver glycogen to synthesize, and blood glucose will not rise, and have improvement liver function to hepatopath and anti-
The effect of fatty liver；The anti-caries characteristic of xylitol effect in all sweeteners is best, and it can not be produced dental caries in oral cavity
The bacterial fermentation of tooth is utilized, and can suppress the generation of streptococcus growth and acid, is prevented carious tooth and is reduced the generation of dental plaque；Xylose
Alcohol provides energy for human body, synthesizes glycogen, reduces the consumption of the protein in fat and hepatic tissue, liver is protected and is repaiied
It is multiple, the generation that ketoboidies is harmful in human body is eliminated, will not be got fat because edible.Nearest Danish scientist finds that xylitol has again
The effect of the suppression Candida albicans of color, the xyranit such as the frequent oral taking chewy piece of women, lozenge can substantially reduce mycotic
The incidence of vaginitis；Daily oral 2~4g xylitols can improve bone density, so as to also have significant pre- to osteoporosis
Anti- effect.These new discoveries greatly strengthen xylitol as the superiority of new type of health sugar, the sale gesture on international market
Head has even overwhelmed Aspartame.From the nineties in last century so far, the global sales of xylitol totally increases 500%.At present
200,000 tons of international market annual requirement, but annual production is only 70,000 tons or so.Domestic market production capacity is about 2.5 ten thousand tons/year,
In recent years, with the increasing of imbalance between supply and demand, xylitol price continuous rise.
China's xylitol is produced as conventional production methods, mainly using corncob as raw material, is pre-processed using sour water solution, through many
Secondary refined xylose, then xylitol is produced using hydrogenation technology, exist yield poorly, seriously polluted, high consumption, production cost height etc. it is scarce
Point.And Raney nickel pollutes the environment, serious restriction enterprise development and international competitiveness.And utilize microorganism direct fermentation half
Cellulosic hydrolysate produces xylitol, and process conditions are gentle, energy consumption is low, environmental pollution is small, Product quality and safety is reliable.Bamboo shoots add
In general work leftover bits and pieces does not develop, and it makes a fire and all feels trouble peasant Lian Na, also takes up the post of it and rots over time,
Crumb into dust fertilizer.Bamboo shoot process leftover bits and pieces is rich in hemicellulose in fact, and it will be good to produce xylose or even xylitol with it
Raw material, it is with low cost, have no relevant report both at home and abroad at present.
The content of the invention
In order to expand raw material sources, cost is reduced, a kind of raw material of present invention offer are novel, reaction condition is gentle, cost
It is low, green production, environment-friendly, it is easy to accomplish the preparation method of industrialized xylitol.
The present invention is adopted the following technical scheme that：
A kind of method that utilization bamboo shoot process discarded object prepares xylitol, is carried out as follows：
(1) hydrolyze：By bamboo shoot process discarded object, crush, be placed in hydrolysis kettle after drying, add the Compositions of Bamboo Shoot Shell quality 3
~4 times of water, boils 0.5wt%~1wt% sulphur that 5~6 times of the Compositions of Bamboo Shoot Shell quality is added after 100~120min, water removal
5~6h of hydrolysis is boiled under aqueous acid, normal pressure, hydrolyzate is obtained；
(2) neutralize：Hydrolyzate obtained by step (1) is warming up to 75~80 DEG C, CaCO is added while stirring3During emulsion is carried out
Be neutralized to pH for 3.5~4.0, be incubated 70~80min, filter cleaner obtains liquid glucose；
(3) decolourize：1/5~1/7 times of most raw sugar liquid product that liquid glucose obtained by step (2) is concentrated under reduced pressure, filters out precipitation
Solid after, be warming up to 75~80 DEG C, adjust pH to be 2.5~3.5 with hydrochloric acid, atlapulgite is added while stirring and is decolourized, it is de-
Atlapulgite is filtered out after color, the liquid glucose after being decolourized；
(4) ion exchange：Liquid glucose after to being decolourized obtained by step (3) carries out ion exchange, using highly acidic cation tree
Fat and highly basic porous anion resin carry out cross processing, and the liquid glucose after collection of ions exchange is detected by chemical detection method
The concentration of xylose in liquid glucose after ion exchange；
(5) ferment：Culture medium is prepared first：By the liquid glucose after ion exchange obtained by step (4), yeast extract, albumen
Peptone, water mixing i.e. obtains culture medium, by candida tropicalis seed liquor under conditions of 200~220r/min, 28~32 DEG C in
Ferment 48~60h in culture medium, obtains zymotic fluid, gained zymotic fluid is then centrifuged into 15~20 points under 8000~10000rpm
Clock, takes supernatant, adds atlapulgite, pH is to 4.8~5.2 for regulation, boils, is cooled to room temperature, then post-treated obtains product
Xylitol；In the culture medium, through preparation cause the liquid glucose in xylose Theoretical Mass in the medium final concentration of 150
~200g/L, the yeast extract, the final concentration of peptone each stand alone as 8~12g/L, and solvent is water, initial pH5~6；
The volume of the candida tropicalis seed liquor is the 5%~15% of the culture volume；The quality of the atlapulgite
Consumption is calculated as 25~30g/L with the volume of the supernatant.
Further, bamboo shoot process discarded object of the present invention includes Compositions of Bamboo Shoot Shell and discarded bamboo shoots leftover pieces.
Further, in step (1) of the present invention, recommend to be crushed to after the bamboo shoot process discarded object drying granularity for 2~
3mm。
Further, in step (2), because the hydrolyzate is still containing remaining sulfuric acid, pH value is 2.5 or so, therefore is added
CaCO3Emulsion is neutralized.
Further, the CaCO3Emulsion Baume degrees is recommended as 15~17 degree.
Further, in step (3), carried out because the liquid glucose color after concentration is deeper, therefore using atlapulgite at decolouring
Reason, the transparency (diopter) of the liquid glucose is 30%~40% after decolouring.
Further, in step (3), the quality consumption of the atlapulgite be liquid glucose quality after the concentration 12%~
15%.
Further, in step (4) of the present invention, the method for the cross processing is：First with the resin cation to decolourizing
Liquid glucose afterwards carries out ion exchange, then carries out ion exchange with the resin anion (R.A.), as a cycle, repeats 2
~3 times, obtain the liquid glucose after ion exchange.
In step (4), the liquid glucose is further purified by ion-exchange treatment, the transparency (folding of the liquid glucose can be made
Luminosity) up to 95%~97%, liquid glucose is in colorless and transparent.
Further, in step (4) of the present invention, the chemical detection method is DNS methods.
In step (5), before culture medium is prepared, xylose in the liquid glucose can be measured by ultraviolet-visible spectrophotometry
Content so that prepare culture medium when, the final concentration of xylose can be made to meet concentration model of the present invention by regulating pondage
Enclose.
Further, in step (5) of the present invention, the post processing is：Suction filtration, takes filtrate evaporated under reduced pressure at 50~60 DEG C to obtain
Crystal is separated out to solid matter, and after extracting to obtain extract, cooling with absolute ethyl alcohol at 50~55 DEG C, crystal is collected by filtration simultaneously
Dry, obtain the product xylitol.
Further, in step (5) of the present invention, the quality consumption of the atlapulgite is calculated as 25 with the volume of the supernatant
~30g/L.
Further, candida tropicalis seed liquor of the present invention is made as follows：
(1) inclined-plane culture：Candida tropicalis is seeded to slant medium, 30 DEG C are cultivated 3~4 days, obtain inclined-plane
Thalline, the slant medium final concentration, which is constituted, is:1.5~5g/L of yeast extract, 2~5g/L of peptone, 18~25g/ of agar powder
L, xylose 30~50g/L, initial pH 5~6, solvent is water；
(2) seed culture：Selected from inclined-plane thalline during a ring thalline is seeded to seed culture medium, 30 DEG C, 200rpm cultures
24h, obtains candida tropicalis seed liquor；The seed culture medium final concentration is constituted：Glucose 5~15g/L, D- xylose 5
~15g/L, 1.5~5g/L of yeast extract, 2~5g/L of peptone, brewer's wort 2~5g/L, initial pH 5~6, solvent is water.
Further, slant medium final concentration composition of the present invention is preferably：Yeast extract 2g/L, peptone 3g/L, fine jade
Cosmetics 20g/L, xylose 40g/L, solvent are water, pH 5.5.
Further, the seed culture medium concentration composition is preferably：Glucose 10g/L, D- xylose 10g/L, yeast extract
2.0g/L, peptone 3.0g/L, brewer's wort 3.0g/L, pH 5.5, solvent is water.
Further, candida tropicalis (Candida tripicalis, fungi preservation number of the present invention：
AS2.1776), purchased from China General Microbiological DSMZ (Beijing), bioconversion xylitol is carried out, the torrid zone is false
Silk yeast is the form of seed liquor.Constituent analysis is carried out to gained zymotic fluid, wherein, xylose determining alcohol is 80g/L~90g/L,
Remaining xylose concentration is 5g/L~10g/L, and xylitol conversion rate is 62%~66%.
This project is intended using bamboo shoot process discarded object as raw material, produces xylitol using biotransformation method, can save and repeatedly carry
Pure, purification step, improves xylose utilization rate and xylitol conversion rate, realizes that the key technology of xylitol low cost breaks through, increase
Farmers' income, improves the international occupancy volume of China's xylitol and competitiveness.
The advantage of the present invention compared with prior art：
1) the use of bamboo shoot process discarded object is raw material, at home and abroad has no report and the application of correlation；
2) hydrolysis is to carry out at normal temperatures, it is to avoid the danger that height temperate zone is come, and compares color with high temperature and high pressure hydrolysis liquid phase
It is shallower, it is follow-up to decolourize to be more prone to；
3) overall production process of the invention reaction is gentle, it is not necessary to use chemical catalyst, environmental pollution compared with
Small, due to the selectivity that the characteristic and microbial enzyme of microorganism fungus kind are acted on, reacting final product is single so that extract and refined appearance
Easily, production cost is low；
4) the residue main component after zymotic fluid centrifugation is single cell protein, can further be developed.
Embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This.
Embodiment 1
(1) selection of raw material
Make raw material from bamboo shoot process discarded object；
(2) hydrolyze
The bamboo shoot process discarded object chosen is cleaned up with cleaning machine, crushed after drying, the bamboo shoot process after crushing
Discarded object (granularity is 3mm) 100g is put into hydrolysis kettle, adds 300g water, be warming up to 100 DEG C boil 100min, draining after add
500g 0.5wt% sulfuric acid, then heats under 100 DEG C, normal pressure and hydrolyzes 4h, obtain hydrolyzate；
(3) neutralize
Gained hydrolyzate is still containing remaining sulfuric acid, and pH value is 2.5 or so, therefore adds 15 degree of CaCO of Baume degrees3Emulsion is entered
Row is neutralized, and is concretely comprised the following steps：The hydrolyzate is added in neutralizing tank, 80 DEG C are warming up to, described CaCO is added while stirring3
Emulsion, regulation and control to pH are 3.5, are abundant precipitation, are incubated 60 minutes after neutralization, refilter slagging-off, obtain liquid glucose；
(4) evaporate
Liquid glucose after slagging-off is evaporated under reduced pressure, 100mL is concentrated into, and by the CaSO of precipitation4Filter out；
(5) decolourize
Liquid glucose color after concentration is deeper, and decolorization is carried out using atlapulgite 12g：The liquid glucose is heated to 80
DEG C, regulation and control pH is 2.5, and atlapulgite is added while stirring and is decolourized, and atlapulgite, the sugar after gained decolouring are filtered out after decolouring
Liquid transparency (diopter) is 36%；
(6) ion exchange
In order to further purify liquid glucose, ion exchange need to be carried out, it is strong from 732 type strong acidic ion resins and D201 types
Alkali porous anion resin carries out intersection ion-exchange treatment, a diameter of 4cm of chromatographic column, and post bed height is 42cm, xylitol liquid
Flow control is 1.5mL/cm2·min.It is described intersect ion-exchange treatment method be：First carried out with the resin cation
Ion exchange, then ion exchange is carried out with the resin anion (R.A.), as a cycle, repeat 2 times, liquid glucose can be made saturating
Lightness (diopter) is up to 95% or so, and liquid glucose detects that the concentration for obtaining xylose in liquid glucose is in colorless and transparent through DNS methods
375g/L；
(7) ferment：Culture medium is prepared first：By the liquid glucose after 40mL ion exchanges, 0.8g yeast extracts, 0.8g albumen
Peptone, the mixing of 60mL water are obtained in culture medium, gained culture medium, and the xylose in the liquid glucose is caused by calculating preparation culture medium
Final concentration of 150g/L, the yeast extract, the final concentration of peptone are 8g/L, after culture medium is prepared, ultra-clean
Platform accesses 5mL candida tropicalis seed liquors, is then placed in shaking table, ferment 48h under conditions of 200r/min, 30 DEG C, obtains
To zymotic fluid, then gained zymotic fluid is centrifuged 20 minutes under 8000rpm, supernatant is taken, add 2.0g atlapulgites, adjust
PH is saved to 4.8, boils, is cooled to room temperature, suction filtration takes filtrate evaporated under reduced pressure at 50 DEG C to obtain solid matter 13.6g, and 50 DEG C
Solid matter obtained by lower use 60mL absolute ethyl alcohols extracting, separates out crystal after extract cooling, crystal and drying is collected by filtration, pass through
The detection of DNS methods obtains product xylitol 8.3g；
The candida tropicalis seed liquor is obtained as follows：
(1) inclined-plane culture：Candida tropicalis AS2.1776 is seeded to slant medium, 30 DEG C are cultivated 3 days, are obtained
Inclined-plane thalline, the slant medium final concentration, which is constituted, is：Yeast extract 2g/L, peptone 3g/L, agar powder 20g/L, xylose
40g/L, solvent is water, pH 5.5；
(2) seed culture：Selected from inclined-plane thalline during a ring thalline is seeded to seed culture medium, 30 DEG C, 200rpm cultures
24h, obtains seed liquor；The seed culture medium concentration is constituted：Glucose 10g/L, D- xylose 10g/L, yeast extract 2.0g/L,
Peptone 3.0g/L, brewer's wort 3.0g/L, pH 5.5, solvent is water；
In above-mentioned fermentation process, constituent analysis is carried out to the zymotic fluid of gained, wherein xylose determining alcohol is 83g/L, remaining
Xylose concentration is 12g/L, and xylitol conversion rate is 62%.
Embodiment 2
(1) selection of raw material
Make raw material from bamboo shoot process discarded object；
(2) hydrolyze
The bamboo shoot process discarded object chosen is cleaned up with cleaning machine, crushed after drying, the bamboo shoot process after crushing
Discarded object (granularity is 3mm) 100g is put into hydrolysis kettle, adds 300g water, be warming up to 100 DEG C boil 120min, draining after add
500g 0.75wt% sulfuric acid, then heats under 100 DEG C, normal pressure and hydrolyzes 5h, obtain hydrolyzate；
(3) neutralize
Gained hydrolyzate is still containing remaining sulfuric acid, and pH value is 2.5 or so, therefore adds 15 degree of CaCO of Baume degrees3Emulsion is entered
Row is neutralized, and is concretely comprised the following steps：The hydrolyzate is added in neutralizing tank, 80 DEG C are warming up to, described CaCO is added while stirring3
Emulsion, regulation and control to pH are 3.5, are abundant precipitation, are incubated 60 minutes after neutralization, refilter slagging-off, obtain liquid glucose；(4) evaporate
Liquid glucose after slagging-off is evaporated under reduced pressure, 100mL is concentrated into, and by the CaSO of precipitation4Filter out；
(5) decolourize
Liquid glucose color after concentration is deeper, and decolorization is carried out using atlapulgite 12g：The liquid glucose is heated to 80
DEG C, regulation and control pH is 2.5, and atlapulgite is added while stirring and is decolourized, and atlapulgite, the sugar after gained decolouring are filtered out after decolouring
Liquid transparency (diopter) is 37%；
(6) ion exchange
In order to further purify liquid glucose, ion exchange need to be carried out, it is strong from 732 type strong acidic ion resins and D201 types
Alkali porous anion resin carries out intersection ion-exchange treatment, a diameter of 4cm of chromatographic column, and post bed height is 42cm, xylitol liquid
Flow control is 1.5mL/cm2·min.It is described intersect ion-exchange treatment method be：First carried out with the resin cation
Ion exchange, then ion exchange is carried out with the resin anion (R.A.), as a cycle, repeat 2 times, liquid glucose can be made saturating
Lightness (diopter) is up to 95% or so, and liquid glucose detects that the concentration for obtaining xylose in liquid glucose is in colorless and transparent through DNS methods
384g/L；
(7) ferment：Culture medium is prepared first：By the liquid glucose after 47mL ion exchanges, 1.0g yeast extracts, 1.0g albumen
Peptone, the mixing of 53mL water are obtained in culture medium, gained culture medium, the wood in the liquid glucose is caused by calculating preparation culture medium
The final concentration of 180g/L of sugar, the yeast extract, the final concentration of peptone are 10g/L, after culture medium is prepared, super
Net platform access 5mL candida tropicalis seed liquors, are then placed in shaking table, ferment 48h under conditions of 220r/min, 30 DEG C,
Zymotic fluid is obtained, then centrifuges gained zymotic fluid 20 minutes under 10000rpm, supernatant is taken, 2.5g atlapulgites are added,
PH is adjusted to 4.8, boils, is cooled to room temperature, suction filtration takes filtrate evaporated under reduced pressure at 50 DEG C to obtain solid matter 17.9g, and 50
With the solid matter obtained by the extracting of 60mL absolute ethyl alcohols at DEG C, crystal is separated out after extract cooling, crystal and drying is collected by filtration,
Product xylitol 10.7g is obtained through the detection of DNS methods；
In above-mentioned fermentation process, constituent analysis is carried out to the zymotic fluid of gained, wherein xylose determining alcohol is 107g/L, remaining
Xylose concentration is 13g/L, and xylitol conversion rate is 66%.
Embodiment 3
(1) selection of raw material
Make raw material from bamboo shoot process discarded object；
(2) hydrolyze
The bamboo shoot process discarded object chosen is cleaned up with cleaning machine, crushed after drying, the bamboo shoot process after crushing
Discarded object (granularity is 3mm) 100g is put into hydrolysis kettle, adds 300g water, be warming up to 100 DEG C boil 100min, draining after add
500g 1.0wt% sulfuric acid, then heats under 100 DEG C, normal pressure and hydrolyzes 4h, obtain hydrolyzate；
(3) neutralize
Gained hydrolyzate is still containing remaining sulfuric acid, and pH value is 2.5 or so, therefore adds 15 degree of CaCO of Baume degrees3Emulsion is entered
Row is neutralized, and is concretely comprised the following steps：The hydrolyzate is added in neutralizing tank, 80 DEG C are warming up to, described CaCO is added while stirring3
Emulsion, regulation and control to pH are 3.5, are abundant precipitation, are incubated 60 minutes after neutralization, refilter slagging-off, obtain liquid glucose；(4) evaporate
Liquid glucose after slagging-off is evaporated under reduced pressure, 100mL is concentrated into, and by the CaSO of precipitation4Filter out；
(5) decolourize
Liquid glucose color after concentration is deeper, and decolorization is carried out using atlapulgite 15g：The liquid glucose is heated to 80
DEG C, regulation and control pH is 2.5, and atlapulgite is added while stirring and is decolourized, and atlapulgite, the sugar after gained decolouring are filtered out after decolouring
Liquid transparency (diopter) is 38%；
(6) ion exchange
In order to further purify liquid glucose, ion exchange need to be carried out, it is strong from 732 type strong acidic ion resins and D201 types
Alkali porous anion resin carries out intersection ion-exchange treatment, a diameter of 4cm of chromatographic column, and post bed height is 42cm, xylitol liquid
Flow control is 1.5mL/cm2·min.It is described intersect ion-exchange treatment method be：First carried out with the resin cation
Ion exchange, then ion exchange is carried out with the resin anion (R.A.), as a cycle, repeat 2 times, liquid glucose can be made saturating
Lightness (diopter) is up to 95% or so, and liquid glucose detects that the concentration for obtaining xylose in liquid glucose is in colorless and transparent through DNS methods
388g/L；
(7) ferment：Culture medium is prepared first：By the liquid glucose after 52mL ion exchanges, 1.0g yeast extracts, 1.0g albumen
Peptone, the mixing of 48mL water are obtained in culture medium, gained culture medium, the wood in the liquid glucose is caused by calculating preparation culture medium
The final concentration of 200g/L of sugar, the yeast extract, the final concentration of peptone are 10g/L, after culture medium is prepared, super
Net platform access 5mL candida tropicalis seed liquors, are then placed in shaking table, ferment 48h under conditions of 220r/min, 30 DEG C,
Zymotic fluid is obtained, then centrifuges gained zymotic fluid 20 minutes under 10000rpm, supernatant is taken, 2.5g atlapulgites are added,
PH is adjusted to 4.8, boils, is cooled to room temperature, suction filtration takes filtrate evaporated under reduced pressure at 50 DEG C to obtain solid matter 20.9g, and 50
With the solid matter obtained by the extracting of 60mL absolute ethyl alcohols at DEG C, crystal is separated out after extract cooling, crystal and drying is collected by filtration,
Product xylitol 11.4g is obtained through the detection of DNS methods；
In above-mentioned fermentation process, constituent analysis is carried out to the zymotic fluid of gained, wherein xylose determining alcohol is 114g/L, remaining
Xylose concentration is 16g/L, and xylitol conversion rate is 64%.
Embodiment 4
(1) selection of raw material
Make raw material from bamboo shoot process discarded object；
(2) hydrolyze
The bamboo shoot process discarded object chosen is cleaned up with cleaning machine, crushed after drying, the bamboo shoot process after crushing
Discarded object (granularity is 3mm) 100g is put into hydrolysis kettle, adds 400g water, be warming up to 100 DEG C boil 120min, draining after add
600g 1.0wt% sulfuric acid, then heats under 100 DEG C, normal pressure and hydrolyzes 6h, obtain hydrolyzate；
(3) neutralize
Gained hydrolyzate is still containing remaining sulfuric acid, and pH value is 2.5 or so, therefore adds 17 degree of CaCO of Baume degrees3Emulsion is entered
Row is neutralized, and is concretely comprised the following steps：The hydrolyzate is added in neutralizing tank, 60 DEG C are warming up to, described CaCO is added while stirring3
Emulsion, regulation and control to pH are 4.0, are abundant precipitation, are incubated 80 minutes after neutralization, refilter slagging-off, obtain liquid glucose；(4) evaporate
Liquid glucose after slagging-off is evaporated under reduced pressure, 100mL is concentrated into, and by the CaSO of precipitation4Filter out；
(5) decolourize
Liquid glucose color after concentration is deeper, and decolorization is carried out using atlapulgite 15g：The liquid glucose is heated to 75
DEG C, regulation and control pH is 3.5, and atlapulgite is added while stirring and is decolourized, and atlapulgite, the sugar after gained decolouring are filtered out after decolouring
Liquid transparency (diopter) is 40%；
(6) ion exchange
In order to further purify liquid glucose, ion exchange need to be carried out, it is strong from 732 type strong acidic ion resins and D201 types
Alkali porous anion resin carries out intersection ion-exchange treatment, a diameter of 4cm of chromatographic column, and post bed height is 42cm, xylitol liquid
Flow control is 1.5mL/cm2·min.It is described intersect ion-exchange treatment method be：First carried out with the resin cation
Ion exchange, then ion exchange is carried out with the resin anion (R.A.), as a cycle, repeat 3 times, liquid glucose can be made saturating
Lightness (diopter) is up to 97% or so, and liquid glucose detects that the concentration for obtaining xylose in liquid glucose is in colorless and transparent through DNS methods
398g/L；
(7) ferment：Culture medium is prepared first：By the liquid glucose after 50mL ion exchanges, 1.5g yeast extracts, 1.5g albumen
Peptone, the mixing of 50mL water are obtained in culture medium, gained culture medium, and the xylose in the liquid glucose is caused by calculating preparation culture medium
Final concentration of 200g/L, the yeast extract, the final concentration of peptone are 15g/L, after culture medium is prepared, ultra-clean
Platform accesses 15mL candida tropicalis seed liquors, is then placed in shaking table, ferment 60h under conditions of 220r/min, 32 DEG C, obtains
To zymotic fluid, then gained zymotic fluid is centrifuged 15 minutes under 10000rpm, supernatant is taken, 2.5g atlapulgites are added, adjusted
PH is saved to 5.2, boils, is cooled to room temperature, suction filtration takes filtrate evaporated under reduced pressure at 60 DEG C to obtain solid matter 20.4g, and 55 DEG C
Solid matter obtained by lower use 60mL absolute ethyl alcohols extracting, separates out crystal after extract cooling, crystal and drying is collected by filtration, pass through
The detection of DNS methods obtains product xylitol 11.3g；
In above-mentioned fermentation process, constituent analysis is carried out to the zymotic fluid of gained, wherein xylose determining alcohol is 113g/L, remaining
Xylose concentration is 13g/L, and xylitol conversion rate is 62%.
Claims (10)
1. a kind of method that utilization bamboo shoot process discarded object prepares xylitol, it is characterised in that methods described is entered as follows
OK：
(1) hydrolyze：By bamboo shoot process discarded object, crush, be placed in hydrolysis kettle after drying, add 3~4 times of the Compositions of Bamboo Shoot Shell quality
Water, boil added after 100~120min, water removal 5~6 times of the Compositions of Bamboo Shoot Shell quality 0.5wt%~1wt% sulfuric acid it is water-soluble
5~6h of hydrolysis is boiled under liquid, normal pressure, hydrolyzate is obtained；
(2) neutralize：Hydrolyzate obtained by step (1) is warming up to 75~80 DEG C, CaCO is added while stirring3Emulsion is neutralized, in
Be 3.5~4.0 to pH, be incubated 60~80min, filter cleaner obtains liquid glucose；
(3) decolourize：1/5~1/7 times of most raw sugar liquid product that liquid glucose obtained by step (2) is concentrated under reduced pressure, filters out consolidating for precipitation
After body, 75~80 DEG C are warming up to, adjusts pH to be 2.5~3.5 with hydrochloric acid, atlapulgite is added while stirring and is decolourized, after decolouring
Atlapulgite is filtered out, the liquid glucose after being decolourized；
(4) ion exchange：Liquid glucose after to decolourizing obtained by step (3) carries out ion exchange, using strong acidic ion resin and
Highly basic porous anion resin carries out cross processing, and the liquid glucose after collection of ions exchange detects ion by chemical detection method
The concentration of xylose in liquid glucose after exchange；
(5) ferment：Culture medium is prepared first：By the liquid glucose after ion exchange obtained by step (4), yeast extract, peptone, water
Mixing i.e. obtain culture medium, by candida tropicalis seed liquor under conditions of 200~220r/min, 28~32 DEG C in culture medium
48~60h of middle fermentation, obtains zymotic fluid, and then gained zymotic fluid is centrifuged 15~20 minutes under 8000~10000rpm, is taken
Supernatant, adds atlapulgite, pH is to 4.8~5.2 for regulation, boils, is cooled to room temperature, then post-treated obtains product xylose
Alcohol；In the culture medium, through preparation cause the liquid glucose in xylose Theoretical Mass in the medium final concentration of 150~
200g/L, the yeast extract, the final concentration of peptone each stand alone as 8~12g/L, and solvent is water, initial pH5~6；Institute
The volume for stating candida tropicalis seed liquor is the 5%~15% of the culture volume.
2. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 1, it is characterised in that the torrid zone
Candida seed liquor is made as follows：
(1) inclined-plane culture：Candida tropicalis is seeded to slant medium, 30 DEG C are cultivated 3~4 days, obtain inclined-plane thalline,
The slant medium final concentration is constituted:1.5~5g/L of yeast extract, 2~5g/L of peptone, 18~25g/L of agar powder, xylose
30~50g/L, initial pH 5~6, solvent is water；
(2) seed culture：Selected from inclined-plane thalline during a ring thalline is seeded to seed culture medium, 30 DEG C, 200rpm culture 24h,
Obtain candida tropicalis seed liquor；The seed culture medium final concentration is constituted：Glucose 5~15g/L, D- xylose 5~
15g/L, 1.5~5g/L of yeast extract, 2~5g/L of peptone, brewer's wort 2~5g/L, initial pH 5~6, solvent is water.
3. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 1, it is characterised in that：Step (1)
In, granularity is crushed to for 2~3mm after the bamboo shoot process discarded object drying.
4. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 1, it is characterised in that：Step (2)
In, the CaCO3Emulsion Baume degrees is 15~17 degree.
5. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 1, it is characterised in that：Step (3)
In, the quality consumption of the atlapulgite is 12%~15% of liquid glucose quality after the concentration.
6. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 1, it is characterised in that：Step (4)
In, the method for the cross processing is：First carry out ion exchange to the liquid glucose after decolouring with the resin cation, then with described
Resin anion (R.A.) carries out ion exchange, as a cycle, repeats 2~3 times, obtains the liquid glucose after ion exchange.
7. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 1, it is characterised in that：Step (5)
In, the quality consumption of the atlapulgite is calculated as 25~30g/L with the volume of the supernatant.
8. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 1, it is characterised in that：Step (5)
In, the post processing is：Suction filtration, takes filtrate evaporated under reduced pressure at 55~60 DEG C to obtain solid matter, and used at 50~55 DEG C
Absolute ethyl alcohol separates out crystal after extracting to obtain extract, cooling, and crystal and drying is collected by filtration, and obtains the product xylitol.
9. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 2, it is characterised in that：The inclined-plane
Culture medium final concentration is constituted：Yeast extract 2g/L, peptone 3g/L, agar powder 20g/L, xylose 40g/L, solvent are water, pH
5.5。
10. the method for preparing xylitol using bamboo shoot process discarded object as claimed in claim 2, it is characterised in that：The kind
Sub- culture medium concentration is constituted：Glucose 10g/L, D- xylose 10g/L, yeast extract 2.0g/L, peptone 3.0g/L, brewer's wort
3.0g/L, pH 5.5, solvent is water.
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| CN111440829A (en) * | 2020-02-27 | 2020-07-24 | 浙江工业大学 | Method for preparing xylitol by using citrus peel |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982460A (en) * | 2005-09-30 | 2007-06-20 | 广西壮族自治区中国科学院广西植物研究所 | Separation of tropical candiyeast strain and production of xylitol |
| US20110097772A1 (en) * | 2009-10-23 | 2011-04-28 | Institute Of Nuclear Energy Research Atomic Energy Council, Executive Yuan | Method for producing xylitol from lignocellulosic hydrolysates without detoxification |
| CN104762333A (en) * | 2015-03-09 | 2015-07-08 | 浙江工业大学 | A method of preparing xylitol by utilization of winter bamboo shoot shells |
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2017
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982460A (en) * | 2005-09-30 | 2007-06-20 | 广西壮族自治区中国科学院广西植物研究所 | Separation of tropical candiyeast strain and production of xylitol |
| US20110097772A1 (en) * | 2009-10-23 | 2011-04-28 | Institute Of Nuclear Energy Research Atomic Energy Council, Executive Yuan | Method for producing xylitol from lignocellulosic hydrolysates without detoxification |
| CN104762333A (en) * | 2015-03-09 | 2015-07-08 | 浙江工业大学 | A method of preparing xylitol by utilization of winter bamboo shoot shells |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111440829A (en) * | 2020-02-27 | 2020-07-24 | 浙江工业大学 | Method for preparing xylitol by using citrus peel |
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