CN107022479B - A kind of bacterial reproduction case for carrying out sample comparison - Google Patents

A kind of bacterial reproduction case for carrying out sample comparison Download PDF

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Publication number
CN107022479B
CN107022479B CN201710410844.7A CN201710410844A CN107022479B CN 107022479 B CN107022479 B CN 107022479B CN 201710410844 A CN201710410844 A CN 201710410844A CN 107022479 B CN107022479 B CN 107022479B
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China
Prior art keywords
pallet
out sample
carrying
bacterial reproduction
sample comparison
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CN201710410844.7A
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Chinese (zh)
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CN107022479A (en
Inventor
胥振国
蔡玉华
袁星
严从权
向敏
宫斌
皇鹤春
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Shenzhen KangTai Biological Products Co., Ltd.
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Hefei Technology College
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Abstract

The invention discloses a kind of bacterial reproduction casees for carrying out sample comparison, including babinet, the upper surface of the babinet is equipped with rectangular port, support column is equipped at the inner bottom surface center of the babinet, the outer surface of the support column is fixedly connected with the first pallet, the upper surface of first pallet is fixedly connected with the first culture dish, the upper surface of the support column is equipped with rotation hole, the inside of the rotation hole is rotatably connected to Rotational Cylindrical by moving block, the outer surface of the Rotational Cylindrical slidably connects the second pallet by connecting hole, the internal slide of the rectangular port is connected with end cap, the end cap is internally provided with swivel becket, the inner side of the swivel becket is equipped with annular groove, the inside of the annular groove is rotatably connected to rotating disc by ring block, the upper surface both sides of the rotating disc are respectively equipped with observation window and addition head.The bacterial reproduction case of the carry out sample comparison can greatly improve the convenience of operation, and compare and study convenient for sample.

Description

A kind of bacterial reproduction case for carrying out sample comparison
Technical field
The present invention relates to breeding box technology field, specially a kind of bacterial reproduction case for carrying out sample comparison.
Background technology
In the Related Experimental Study of fine bacterium, the breeding case of bacterium is indispensable important equipment.In experiment, in order to Bacterial reproduction condition is studied, a variety of different culture dishes generally can be all provided and carry out bacterial reproductions, and existing breeding Case, which is substantially, to be individually placed into culture dish inside breeding case, comparatively laborious in the addition for carrying out breeding raw material, is influenced Working efficiency, and existing breeding case can not carry out sample comparison, need all to take out multiple culture dishes, can not only make Into the variation of Reproduction Conditions, and operation is more complicated, is unfavorable for the progress of experiment.
Invention content
The purpose of the present invention is to provide a kind of bacterial reproduction case for carrying out sample comparison, to solve in above-mentioned background technology The problem of proposition.
To achieve the above object, the present invention provides following technical solution:A kind of bacterial reproduction case for carrying out sample comparison, packet Babinet is included, the appearance surface rear end of the babinet is installed with bacteria detecting apparatus, and the upper surface of the babinet is equipped with square end Mouthful, support column is equipped at the inner bottom surface center of the babinet, the outer surface of the support column is fixedly connected with the first pallet, institute The upper surface for stating the first pallet is fixedly connected with the first culture dish, and the upper surface of the support column is equipped with rotation hole, the rotation The inside in hole is rotatably connected to Rotational Cylindrical by moving block, and the upper surface of the Rotational Cylindrical is equipped with diamond shape slot, the Rotational Cylindrical Outer surface is equipped with splined post, and the outer surface of the Rotational Cylindrical slidably connects the second pallet by connecting hole, the connecting hole Splined hole is internally provided with, the splined hole is slidably connected to the outer surface of splined post, and the upper surface of second pallet, which is fixed, to be connected The second culture dish is connected to, the upper table surface rear end of second pallet is equipped with break slot, the upper surface inside two of second pallet End is equipped with hanging ring, and the internal slide of the rectangular port is connected with end cap.
Preferably, the end cap is internally provided with swivel becket, and the inner side of the swivel becket is equipped with annular groove, the ring The inside of shape slot is rotatably connected to rotating disc by ring block, and the upper surface both sides of the rotating disc are respectively equipped with observation window and add Add head, boss is equipped at the upper surface center of the rotating disc.
Preferably, the boss is internally provided with guide groove, and the internal slide of the guide groove is connected with guide rod, described The lower surface of guide rod is fixedly connected with diamond shape head, and the diamond shape head is slidably connected to the interior surface of diamond shape slot, the guiding The outer surface of bar is rotatably connected to rotor plate, and the lower surface of the rotor plate is fixedly connected with spring.
Preferably, the end cap is square structure.
Preferably, the upper surface of the addition head is threaded with seal cap, and the internal slide of the seal cap is connected with Liquid flowing tube, the upper surface of the liquid flowing tube are fixedly connected with pressing glueballs, and the outer surface upper end of the liquid flowing tube is equipped with pressing plate, institute The lower surface for stating pressing plate is fixedly connected with resetting spring.
Preferably, the guide groove and guide rod are cylindrical structure.
Preferably, the rotor plate is slidably connected to the interior surface of guide groove.
Preferably, the spring noose is in the outer surface of guide rod.
Compared with prior art, the beneficial effects of the invention are as follows:The bacterial reproduction case of the carry out sample comparison passes through rotation Second pallet of connection and the first pallet for being fixedly connected fix culture dish, with reference to break slot, facilitate to different times Sample carries out effective ratio pair, but also can install observation window by the rotating disc of rotation connection, can conveniently adjust observation position It puts, but also comparison can be sampled by liquid flowing tube, be detected using bacteria detecting apparatus, greatly improve operation just Profit, and compare and study convenient for sample.
Description of the drawings
Fig. 1 is the overall structure diagram of the present invention;
Fig. 2 is the vertical view of the end cap of the present invention;
Fig. 3 is the schematic diagram of the second pallet of the present invention;
Fig. 4 is the structure diagram of the Rotational Cylindrical of the present invention;
Fig. 5 is the connection diagram of the guide rod of the present invention;
Fig. 6 is the connection sectional view of the splined post of the present invention;
Fig. 7 is the connection sectional view of the liquid flowing tube of the present invention.
In figure:1 babinet, 11 rectangular ports, 12 support columns, 13 first pallets, 14 first culture dishes, 15 rotation holes, 16 are carefully Bacterium detection device, 2 Rotational Cylindricals, 21 moving blocks, 22 diamond shape slots, 23 splined posts, 24 connecting holes, 25 second pallets, 26 splined holes, 27 Second culture dish, 28 break slots, 29 hanging rings, 3 end caps, 31 swivel beckets, 32 annular grooves, 33 ring blocks, 34 rotating discs, 35 observations Window, 36 addition heads, 4 boss, 41 guide grooves, 42 guide rods, 43 diamond shape heads, 44 rotor plates, 45 springs, 5 seal caps, 51 take liquid Pipe, 52 pressing glueballs, 53 pressing plates, 54 resetting springs.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work Embodiment shall fall within the protection scope of the present invention.
- 7 are please referred to Fig.1, the present invention provides a kind of technical solution:A kind of bacterial reproduction case for carrying out sample comparison, including Babinet 1, the appearance surface rear end of babinet 1 are installed with bacteria detecting apparatus 16, and the upper surface of babinet 1 is equipped with rectangular port 11, Support column 12 is equipped at the inner bottom surface center of babinet 1, the outer surface of support column 12 is fixedly connected with the first pallet 13, the first support The upper surface of disk 13 is fixedly connected with the first culture dish 14, and there are six the first culture dishes 14, and is angularly distributed in the first pallet 13 upper surface can add a variety of different culture solutions, can provide a variety of samples, and the upper surface of support column 12 is equipped with rotation Hole 15, the inside of rotation hole 15 are rotatably connected to Rotational Cylindrical 2 by moving block 21, and the upper surface of Rotational Cylindrical 2 is equipped with diamond shape slot 22, The outer surface of Rotational Cylindrical 2 is equipped with splined post 23, and the outer surface of Rotational Cylindrical 2 slidably connects the second pallet 25 by connecting hole 24, Connecting hole 24 is internally provided with splined hole 26, and splined hole 26 is slidably connected to the outer surface of splined post 23, the second pallet 25 it is upper Surface is fixedly connected with the second culture dish 27, and the upper table surface rear end of the second pallet 25 is equipped with break slot 28, the size of break slot 28 It, can be to sample ratio to more convenient for observing the possessive construction in the first culture dish 14 not less than the size of the first culture dish 14 Adding finely, both ends are equipped with hanging ring 29 on the inside of the upper surface of the second pallet 25, and the internal slide of rectangular port 11 is connected with end cap 3, End cap 3 is square structure, avoids rotating, and improves stability when adjusting, and end cap 3 is internally provided with swivel becket 31, swivel becket 31 Inner side be equipped with annular groove 32, the inside of annular groove 32 is rotatably connected to rotating disc 34, rotating disc 34 by ring block 33 Upper surface both sides be respectively equipped with observation window 35 and addition head 36, addition head 36 upper surface be threaded with seal cap 5, seal The internal slide of cap 5 is connected with liquid flowing tube 51, and the upper surface of liquid flowing tube 51, which is fixedly connected with, presses glueballs 52, outside liquid flowing tube 51 Surface upper end is equipped with pressing plate 53, and the lower surface of pressing plate 53 is fixedly connected with resetting spring 54, can be sealed when not in use, Dust is avoided to fall into and changes breeding environment, after different sample extractions is come out, bacteria detecting apparatus 16 can be added dropwise to Inside carries out plus surveys, and can analyze the breeding situation of bacterium, using it is more convenient efficiently, set at the upper surface center of rotating disc 34 There is boss 4, boss 4 is internally provided with guide groove 41, and guide groove 41 and guide rod 42 are cylindrical structure, turns convenient for opposite Dynamic, the internal slide of guide groove 41 is connected with guide rod 42, and the lower surface of guide rod 42 is fixedly connected with diamond shape head 43, diamond shape head 43 are slidably connected to the interior surface of diamond shape slot 22, and the outer surface of guide rod 42 is rotatably connected to rotor plate 44, and rotor plate 44 is slided The dynamic interior surface for being connected to guide groove 41, can prevent from coming off, and improve stability, the lower surface of rotor plate 44 is fixedly connected with Spring 45,45 noose of spring in this way can jack up guide rod 42 upwards in the outer surface of guide rod 42, can be with Rotational Cylindrical 2 It is detached from, avoids maloperation.
The present invention is in the specific implementation:When in use, can respectively add in the first culture dish 14 and the second culture dish 27 Enter different culture solutions, convenient for providing different breeding samples, hanging ring 29 then can be tangled with hook, it can be by the second pallet 25 are put into babinet 1, while ensure that splined hole 26 is slidably connected to the outer surface of splined post 23, such second pallet 25 As Rotational Cylindrical 2 is had rotated, then end cap 3 can be put into rectangular port 11 and cover babinet 1, avoid the dirts such as dust Contaminate culture solution, when bacterial reproduction for a period of time after, need to add new culture solution or various samples be compared, at this moment may be used Rotating disc 34 to be rotated to certain angle, addition head 36 can be turned to the top of the second culture dish 27, remove seal cap Culture solution can be added or the formational situation of observation bacterium colony is carried out by observation window 35, and needed the first culture dish 14 When being compared with the second culture dish 27, guide rod 42 can be pressed downward, at this moment can by rotor plate 44 come compressed spring 45, Diamond shape head 43 can be inserted into inside diamond shape slot 22, at this moment can be rotated by rotating guide rod 42 with Rotational Cylindrical 2, together When rotor plate 44 do not rotated together with guide rod 42, can be deformed upon to avoid spring 45, improve service life, then Rotational Cylindrical 2 are rotated by splined post 23 with the second pallet 25, and break slot 28 can be turned to the top of the first culture dish 14, Ran Houzai Guide rod 42 is unclamped, spring 45 resets pushes up guide rod 42 upwards, can be detached from Rotational Cylindrical 2, then again will by rotating disc 34 Observation window 35 turns to the top of break slot 28, is thus observed that the first culture dish 14 and the second culture dish 27, together Reason can also add culture solution by adding first 36 into the first culture dish 14, then be rotated successively, can be to multiple samples This is compared, and when being sampled, and can be pressed downward liquid flowing tube 51, can thus may be used with compression reseting spring 54 It to touch culture solution, then squeezes pressing glueballs 52 and discharges internal air, unclamp again hand, pressing glueballs 52 in this way will be because It is restored automatically for elastic reaction, it is internal in this way to generate negative pressure, under atmospheric pressure, culture solution can be drawn into Inside liquid flowing tube 51, then resetting spring 54 will push up liquid flowing tube 51 upwards, so as to leave culture solution, conveniently be sampled inspection It surveys.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace And modification, the scope of the present invention is defined by the appended.

Claims (9)

1. a kind of bacterial reproduction case for carrying out sample comparison, including babinet (1), it is characterised in that:The outer surface of the babinet (1) Rear end is installed with bacteria detecting apparatus (16), and the upper surface of the babinet (1) is equipped with rectangular port (11), the babinet (1) support column (12) is equipped at inner bottom surface center, the outer surface of the support column (12) is fixedly connected with the first pallet (13), the upper surface of first pallet (13) is fixedly connected with the first culture dish (14), the upper surface of the support column (12) Equipped with rotation hole (15), the inside of the rotation hole (15) is rotatably connected to Rotational Cylindrical (2), the rotation by moving block (21) The upper surface of column (2) is equipped with diamond shape slot (22), and the outer surface of the Rotational Cylindrical (2) is equipped with splined post (23), the Rotational Cylindrical (2) Outer surface the second pallet (25) is slidably connected by connecting hole (24), the connecting hole (24) is internally provided with splined hole (26), the splined hole (26) is slidably connected to the outer surface of splined post (23), and the upper surface of second pallet (25) is fixed The second culture dish (27) is connected with, the upper table surface rear end of second pallet (25) is equipped with break slot (28), second pallet (25) both ends are equipped with hanging ring (29) on the inside of upper surface, and the internal slide of the rectangular port (11) is connected with end cap (3).
2. a kind of bacterial reproduction case for carrying out sample comparison according to claim 1, it is characterised in that:The end cap (3) Be internally provided with swivel becket (31), the inner side of the swivel becket (31) is equipped with annular groove (32), the annular groove (32) Inside is rotatably connected to rotating disc (34) by ring block (33), and the upper surface both sides of the rotating disc (34) are respectively equipped with observation Window (35) and addition head (36), boss (4) is equipped at the upper surface center of the rotating disc (34).
3. a kind of bacterial reproduction case for carrying out sample comparison according to claim 2, it is characterised in that:The boss (4) Be internally provided with guide groove (41), the internal slide of the guide groove (41) is connected with guide rod (42), the guide rod (42) Lower surface be fixedly connected with diamond shape head (43), the diamond shape head (43) is slidably connected to the interior surface of diamond shape slot (22), institute The outer surface for stating guide rod (42) is rotatably connected to rotor plate (44), and the lower surface of the rotor plate (44) is fixedly connected with spring (45)。
4. a kind of bacterial reproduction case for carrying out sample comparison according to claim 1, it is characterised in that:First culture There are six wares (14), and is angularly distributed in the upper surface of the first pallet (13).
5. a kind of bacterial reproduction case for carrying out sample comparison according to claim 1, it is characterised in that:The end cap (3) For square structure.
6. a kind of bacterial reproduction case for carrying out sample comparison according to claim 2, it is characterised in that:The addition head (36) upper surface is threaded with seal cap (5), and the internal slide of the seal cap (5) is connected with liquid flowing tube (51), described The upper surface of liquid flowing tube (51) is fixedly connected with pressing glueballs (52), and the outer surface upper end of the liquid flowing tube (51) is equipped with pressing plate (53), the lower surface of the pressing plate (53) is fixedly connected with resetting spring (54).
7. a kind of bacterial reproduction case for carrying out sample comparison according to claim 3, it is characterised in that:The guide groove (41) and guide rod (42) is cylindrical structure.
8. a kind of bacterial reproduction case for carrying out sample comparison according to claim 3, it is characterised in that:The rotor plate (44) it is slidably connected to the interior surface of guide groove (41).
9. a kind of bacterial reproduction case for carrying out sample comparison according to claim 3, it is characterised in that:The spring (45) Noose is in the outer surface of guide rod (42).
CN201710410844.7A 2017-06-04 2017-06-04 A kind of bacterial reproduction case for carrying out sample comparison Active CN107022479B (en)

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CN108264997A (en) * 2018-03-26 2018-07-10 郭庆 A kind of Bacteria Culture assessing instrument
CN108802029B (en) * 2018-05-09 2020-11-27 河北经贸大学 Biological observation device for scientific research
CN109868209B (en) * 2019-04-17 2022-04-15 重庆医药高等专科学校 A mixing device that is arranged in food medicine to examine bacterium quantity in appearance to detect

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KR101504902B1 (en) * 2012-02-29 2015-03-23 가부시키가이샤 제이텍 Cell culture device having culture medium replacement function
CN203559049U (en) * 2013-11-13 2014-04-23 廊坊师范学院 Culture dish tray
CN106085820B (en) * 2016-08-26 2018-03-16 河南科技大学 A kind of Multifunctional petri dish frame
CN206927891U (en) * 2017-06-04 2018-01-26 合肥职业技术学院 A kind of bacterial reproduction case for carrying out sample comparison

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Effective date of registration: 20191212

Address after: No. 6, Nanshan District science and Technology Industrial Park, Shenzhen, Guangdong, Guangdong

Patentee after: Shenzhen KangTai Biological Products Co., Ltd.

Address before: 238000 Anhui city of Hefei province Chaohu City Economic Development Zone South nest Laoshan Hefei Career Technical College

Patentee before: Hefei Vocationl Technical College