CN107014854A - The durothermic detection method of collagen - Google Patents
The durothermic detection method of collagen Download PDFInfo
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- CN107014854A CN107014854A CN201710198769.2A CN201710198769A CN107014854A CN 107014854 A CN107014854 A CN 107014854A CN 201710198769 A CN201710198769 A CN 201710198769A CN 107014854 A CN107014854 A CN 107014854A
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- collagen
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N25/00—Investigating or analyzing materials by the use of thermal means
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Abstract
The invention belongs to a kind of detection method of collagen, and in particular to a kind of durothermic detection method of collagen.Methods described, collagen solution is prepared by collagen to be measured, and by detecting that collagen solution heats forward and backward viscosity, the temperature tolerance of collagen is judged according to viscosity B coefficent, and then judges this collagen if appropriate for blowing casing.The present invention is judged the temperature tolerance of collagen using by detecting that collagen solution heats forward and backward viscosity according to viscosity B coefficent, and then judges this collagen if appropriate for blowing casing；After being summarized to test data, show that viscosity rate of descent exceedes the unsuitable blowing casing of collagen of a certain numerical value；During concrete application, casing is detected using this temperature and time, by calculating viscosity rate of descent this collagen can be drawn if appropriate for blowing casing；Testing cost is low, and quickly and easily the temperature tolerance of collagen can be detected, has important directive function to the production of casing.
Description
Technical field
The invention belongs to a kind of detection method of collagen, and in particular to a kind of durothermic detection method of collagen.
Background technology
Collagen is the intermediate product that Collagent casing for sausages makes, and ox-hide is by a series of glue for being processed into similar dough
Original, then casing is blow molded into by collagen.
Need to make intestines body become ripe through heater meanses such as boilings after Collagent casing for sausages bowel lavage, the temperature tolerance of casing is applied to effect
Fruit has conclusive effect, and current application test can only test to the casing for recording into intestines, examines relatively delayed,
There is no foresight, detection is more bothered.Collagent casing for sausages is formed by collagen blowing, and the temperature tolerance of collagen has directly to casing
Influence.Tradition mainly detects that testing cost is higher to durothermic inspection by differential scanning calorimetry.
The content of the invention
The deficiency of the prior art more than, it is an object of the invention to provide a kind of durothermic detection method of collagen,
It is low with testing cost, can fast and easy the characteristics of detect, have directive function to the production of casing.
The durothermic detection method of collagen of the present invention, prepares collagen solution, by detecting collagen by collagen to be measured
Solution heats forward and backward viscosity, the temperature tolerance of collagen is judged according to viscosity B coefficent, and then judge whether this collagen fits
Close blowing casing.
Main active substances in collagen are collagen fabric, and collagen is dissolved in into fiber after water and disperseed in the solution, this
When solution viscosity it is higher, after heating collagenous fibres can gelatinization dissolving reduce solution viscosity, by calculate viscosity decline ratio
Example is judged the temperature tolerance of collagen.
Wherein：
Described collagen to be measured is collagen extracted from ox-hide, for preparing Collagent casing for sausages.
The mass concentration of described collagen solution is 10-20%.
Described viscosity is detected using viscosimeter.
Preferably, the durothermic detection method of described collagen, step is as follows：
(1) collagen to be measured is prepared into collagen solution, it is standby after stirring；
(2) viscosity of collagen solution is detected；
(3) collagen solution is heated；
(4) collagen solution after the heating obtained by step (3) is cooled to after room temperature, surveys its viscosity；
(5) ratio declined after heating than viscosity before heating, i.e. viscosity rate of descent are calculated；
(6) casing will be blow molded into the same species collagen for preparing collagen solution, records into and heated after intestines, observation heating
Situation.
It is described to be stirred for collagen being uniformly dispersed in water in step (1).
In step (3), described being heated to be is heated using water-bath.
In step (3), described heating-up temperature is 60-80 DEG C.
In step (3), the described heat time is 2-3 hours.
Described viscosity rate of descent is calculated using equation below：
Viscosity * 100% before viscosity rate of descent=(viscosity after viscosity-heating before heating)/heating.
In step (6), whether described observation heating state occurs that boiling falls stove or water boils for observation in heating process
Decortication.
It is highly preferred that the durothermic detection method of described collagen, step is as follows：
(1) collagen to be measured is configured to the collagen solution that mass concentration is 10%, it is standby after stirring；
(2) viscosity of collagen solution is detected with viscosimeter；
(3) collagen solution is put into 60 DEG C of water-baths and heated；
(4) collagen solution after the heating obtained by step (3) is cooled to after room temperature, its viscosity is measured with viscosity；
(5) ratio declined after heating than viscosity before heating, i.e. viscosity rate of descent are calculated, viscosity rate of descent is using following public
Formula is calculated：
Viscosity * 100% before viscosity rate of descent=(viscosity after viscosity-heating before heating)/heating；
The blown casing of collagen of the viscosity rate of descent more than 73% generally occurs that boiling falls stove or water boils decortication.
Compared with prior art, the beneficial effects of the invention are as follows：
1st, the present invention is using by detecting that collagen solution heats forward and backward viscosity, according to heatproof of the viscosity B coefficent to collagen
Property judged, and then judge this collagen if appropriate for blowing casing.Using mutually synthermal and same time to different casing
Detected, after being summarized to test data, show that viscosity rate of descent exceedes the unsuitable blowing casing of collagen of a certain numerical value.
In specific application, casing is detected using this temperature and time, by calculate viscosity rate of descent can draw it is this
Collagen is if appropriate for blowing casing, it is not necessary to is blown, bowel lavage, detected after heating again.
2nd, the method for the invention testing cost is low, quickly and easily the temperature tolerance of collagen can be detected, to glue
Former quality, which has, more fully to be grasped, and has important directive function to the production of casing.
Embodiment
With reference to embodiment, the present invention will be further described, so that those skilled in the art knows more about the present invention,
But and it is not so limited the present invention.
Embodiment 1
The durothermic detection method of described collagen, step is as follows：
(1) collagen to be measured is configured to the collagen solution that mass concentration is 10%, it is standby after stirring；
(2) viscosity of collagen solution is detected with viscosimeter；
(3) collagen solution is put into 60 DEG C of water-baths and heated；
(4) collagen solution after the heating obtained by step (3) is cooled to after room temperature, its viscosity is measured with viscosity；
(5) ratio declined after heating than viscosity before heating, i.e. viscosity rate of descent are calculated, viscosity rate of descent is using following public
Formula is calculated：
Viscosity * 100% before viscosity rate of descent=(viscosity after viscosity-heating before heating)/heating；
Described collagen to be measured is collagen extracted from ox-hide, for preparing Collagent casing for sausages.
Different collagen is detected using above-mentioned condition, after being summarized to test data, viscosity rate of descent is drawn
The blown casing of collagen more than 73% generally occurs that boiling falls stove, water and boils the exception such as decortication in heating process.
This result of the test is verified using differential scanning calorimetry, both results are consistent.In specific application, using this temperature
Degree and time are detected to casing, viscosity rate of descent are calculated, when viscosity rate of descent is more than 73%, you can judge this collagen not
It is adapted to blowing casing, it is not necessary to blown, bowel lavage, detected after heating again.
Embodiment 2
The durothermic detection method of described collagen, step is as follows：
(1) collagen to be measured is configured to the collagen solution that mass concentration is 15%, it is standby after stirring；
(2) viscosity of collagen solution is detected with viscosimeter；
(3) collagen solution is put into 70 DEG C of water-baths and heated；
(4) collagen solution after the heating obtained by step (3) is cooled to after room temperature, its viscosity is measured with viscosity；
(5) ratio declined after heating than viscosity before heating, i.e. viscosity rate of descent are calculated, viscosity rate of descent is using following public
Formula is calculated：
Viscosity * 100% before viscosity rate of descent=(viscosity after viscosity-heating before heating)/heating；
Described collagen to be measured is collagen extracted from ox-hide, for preparing Collagent casing for sausages.
Different collagen is detected using above-mentioned condition, after being summarized to test data, viscosity rate of descent is drawn
The blown casing of collagen more than 79% generally occurs that boiling falls stove, water and boils the exception such as decortication in heating process.
This result of the test is verified using differential scanning calorimetry, both results are consistent.In specific application, using this temperature
Degree and time are detected to casing, viscosity rate of descent are calculated, when viscosity rate of descent is more than 79%, you can judge this collagen not
It is adapted to blowing casing, it is not necessary to blown, bowel lavage, detected after heating again.
Embodiment 3
The durothermic detection method of described collagen, step is as follows：
(1) collagen to be measured is configured to the collagen solution that mass concentration is 20%, it is standby after stirring；
(2) viscosity of collagen solution is detected with viscosimeter；
(3) collagen solution is put into 80 DEG C of water-baths and heated；
(4) collagen solution after the heating obtained by step (3) is cooled to after room temperature, its viscosity is measured with viscosity；
(5) ratio declined after heating than viscosity before heating, i.e. viscosity rate of descent are calculated, viscosity rate of descent is using following public
Formula is calculated：
Viscosity * 100% before viscosity rate of descent=(viscosity after viscosity-heating before heating)/heating；
Described collagen to be measured is collagen extracted from ox-hide, for preparing Collagent casing for sausages.
Different collagen is detected using above-mentioned condition, after being summarized to test data, viscosity rate of descent is drawn
The blown casing of collagen more than 87% generally occurs that boiling falls stove, water and boils the exception such as decortication in heating process.
This result of the test is verified using differential scanning calorimetry, both results are consistent.In specific application, using this temperature
Degree and time are detected to casing, viscosity rate of descent are calculated, when viscosity rate of descent is more than 87%, you can judge this collagen not
It is adapted to blowing casing, it is not necessary to blown, bowel lavage, detected after heating again.
Claims (10)
1. a kind of durothermic detection method of collagen, it is characterised in that：Collagen to be measured is prepared into collagen solution, by detecting collagen
Solution heats forward and backward viscosity, the temperature tolerance of collagen is judged according to viscosity B coefficent, and then judge whether this collagen fits
Close blowing casing.
2. the durothermic detection method of collagen according to claim 1, it is characterised in that：Described collagen to be measured is from ox
Collagen extracted in skin, for preparing Collagent casing for sausages.
3. the durothermic detection method of collagen according to claim 1, it is characterised in that：The quality of described collagen solution
Concentration is 10-20%.
4. the durothermic detection method of collagen according to claim 1, it is characterised in that：Described viscosity uses viscosimeter
Detected.
5. the durothermic detection method of collagen according to claim 1, it is characterised in that：Step is as follows：
(1) collagen to be measured is prepared into collagen solution, it is standby after stirring；
(2) viscosity of collagen solution is detected；
(3) collagen solution is heated；
(4) collagen solution after the heating obtained by step (3) is cooled to after room temperature, surveys its viscosity；
(5) ratio declined after heating than viscosity before heating, i.e. viscosity rate of descent are calculated；
(6) collagen to be measured is blow molded into casing, records into and heated after intestines, observe heating state.
6. the durothermic detection method of collagen according to claim 5, it is characterised in that：In step (3), described heating
To be heated using water-bath.
7. the durothermic detection method of collagen according to claim 5, it is characterised in that：In step (3), described heating
Temperature is 60-80 DEG C.
8. the durothermic detection method of collagen according to claim 5, it is characterised in that：In step (3), described heating
Time is 2-3 hours.
9. the durothermic detection method of collagen according to claim 5, it is characterised in that：Described viscosity rate of descent is used
Equation below is calculated：
Viscosity * 100% before viscosity rate of descent=(viscosity after viscosity-heating before heating)/heating.
10. the durothermic detection method of collagen according to claim 5, it is characterised in that：In step (6), described sight
Examine whether heating state occurs that boiling falls stove or water boils decortication for observation in heating process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710198769.2A CN107014854A (en) | 2017-03-29 | 2017-03-29 | The durothermic detection method of collagen |
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| CN201710198769.2A CN107014854A (en) | 2017-03-29 | 2017-03-29 | The durothermic detection method of collagen |
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| CN107014854A true CN107014854A (en) | 2017-08-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112526069A (en) * | 2020-11-25 | 2021-03-19 | 山东海奥斯生物科技有限公司 | Method for judging quality of collagen fiber |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621213A (en) * | 2012-01-13 | 2012-08-01 | 淮海工学院 | Evaluation method for degree of thermal denaturation of macromolecular collagen by using pepsin process |
| CN104430787A (en) * | 2014-11-20 | 2015-03-25 | 广西大学 | Fish skin collagen casing and preparation method thereof |
| CN105061587A (en) * | 2015-08-26 | 2015-11-18 | 华南理工大学 | Ultrasonic wave aided extraction method suitable for high-viscosity collagen |
-
2017
- 2017-03-29 CN CN201710198769.2A patent/CN107014854A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621213A (en) * | 2012-01-13 | 2012-08-01 | 淮海工学院 | Evaluation method for degree of thermal denaturation of macromolecular collagen by using pepsin process |
| CN104430787A (en) * | 2014-11-20 | 2015-03-25 | 广西大学 | Fish skin collagen casing and preparation method thereof |
| CN105061587A (en) * | 2015-08-26 | 2015-11-18 | 华南理工大学 | Ultrasonic wave aided extraction method suitable for high-viscosity collagen |
Non-Patent Citations (3)
| Title |
|---|
| 付丽红: "胶原蛋白在不同条件下的粘度变化", 《日用化学工业》 * |
| 张小娟: "牛皮胶原蛋白的水解及其抗氧化性研究", 《中国优秀硕士论文全文数据库 工程科技Ⅰ辑》 * |
| 张强: "不同方法提取鲢鱼皮胶原蛋白的理化特性比较", 《现代食品科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112526069A (en) * | 2020-11-25 | 2021-03-19 | 山东海奥斯生物科技有限公司 | Method for judging quality of collagen fiber |
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Application publication date: 20170804 |
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