CN107012196A - Microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow - Google Patents

Microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow Download PDF

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Publication number
CN107012196A
CN107012196A CN201710341977.3A CN201710341977A CN107012196A CN 107012196 A CN107012196 A CN 107012196A CN 201710341977 A CN201710341977 A CN 201710341977A CN 107012196 A CN107012196 A CN 107012196A
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CN
China
Prior art keywords
experimental method
inner cylinder
microorganism
outer barrel
shear flow
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CN201710341977.3A
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Chinese (zh)
Inventor
曾利
谭水位
陈小莉
吴红
吴一红
赵懿珺
纪平
张博
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China Institute of Water Resources and Hydropower Research
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China Institute of Water Resources and Hydropower Research
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Priority to CN201710341977.3A priority Critical patent/CN107012196A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms

Abstract

Microorganism hydrodynamics experimental method under the conditions of a kind of cyclical level shear flow, it is characterised in that the experimental method includes:Using the tank of a loop configuration, Experimental Flowing Object is added in the tank, by driving the inner cylinder and outer barrel of annular water tank to carry out the rotation of reverse direction, the flow velocity and fluidised form of shear flow in tank is formed and change.Microorganism hydrodynamics experimental method under the conditions of the cyclical level shear flow is simple and practical, experiment condition can fully be met, and it can realize the horizontal shear hydraulics condition of long-time circular flow, each mechanism of device hardly produces disturbance to water body, stability is good, and the water body of different densities need not be configured, also injury will not be produced to the microorganism in water body in the process of running.

Description

Microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow
Technical field
The invention belongs to microorganism hydrodynamics experimental technique, it is specifically related to a kind of for moving algae aggregation simulation in water Microorganism hydrodynamics experimental method under the conditions of the cyclical level shear flow of experiment.
Background technology
Wawter bloom (Algal Blooms) refers to a kind of natural, ecological phenomenon of algal bloom in poisons in freshwater, is that water body is rich A kind of feature of nutrient laden, enters after water body mainly due to the effluent sewage containing a large amount of nitrogen, phosphorus in life and industrial and agricultural production, blue Make water body that a kind of phenomenon of blueness or green is presented after the amount reproductions such as algae, green alga, diatom.In recent years, China is much regional, example Such as Taihu Lake, Dian Chi, Chaohu, Hongchehu Lake, the tributary of reservoir area of Three Gorges, extensive, " wawter bloom " phenomenon of high intensity has all been broken out." water China " brings many harm to public health and socio-economic development, including threaten drinking for resident as a kind of ecocatas-trophe Water security, increases water treatment cost;Biotoxin is discharged, in the multistage for triggering fish, animal and the public by food chain Poison;A large amount of consumption dissolved oxygens, make the aquatic organism hypoxia death such as fish, shrimp, destroy culture fishery;Distribute stench, infringement tourism, sight The development of light industry." wawter bloom " has turned into that China is current and very long one period great water environmental problems of interest from now on, National water pollution control with administer pay close attention to problem.
The hydraulic analogy experimental provision of algae aggregation is " wawter bloom " life in the environment waters such as research lake, reservoir, river course Disappear the important hardware platform of evolution process, to the hydrodynamic force mechanism of clear and definite " wawter bloom " formation, sets up efficient " wawter bloom " prevention, control System is significant with control measures, is the emphasis that the scientific research institutions such as water conservancy, environment, ecology pay close attention to always.Present day analog algae The experimental provision of class aggregation is mainly used in reflecting the influence of temperature, illumination, nutritive salt to wawter bloom, is only applicable to this ideal of hydrostatic Hydrodynamic condition.However, the hydrodynamic condition that " wawter bloom " occurs in actual conditions is generally river channels, such as lake, storehouse, gulf Wind current, reservoir area main flow and density current caused by the temperature difference of tributary, the natural flowing in river course etc..Under circumstance of flowing water and Lentic environment There are significant differences in flow shape, major embodiment is both ways:One is that current under river channels are generally turbulent flow fluidised form, and hydrostatic Condition is non-turbulent flow fluidised form;Two be that current have obvious velocity profile under river channels.Existing algae aggregation simulation Experimental provision can not reflect the feature physical quantity of dynamic water, such as time averaged velocity gradient, tubulence energy, turbulence dissipative shock wave, poly- to algae The influence of collection, and then the forming process and characteristic rule of " wawter bloom " can not be truly simulated in circumstance of flowing water.Therefore, utilization can be simulated The experimental provision of density current condition, to study algae accumulation rule, to understanding under river channels the hydrodynamic(al) that " wawter bloom " formation is developed Power mechanism, scientific and reasonable " wawter bloom " prevention of structure, control are significant with control measures, are also current environment and ecology Field of hydraulics studies key issue urgently to be resolved hurrily.
Complex device being used the test method of simulation shear flow at present, such as long tank detaches iris type shearing more Stream generation apparatus, the long reverse water-feeding type shear flow experimental rig of tank, belt pulley reverse movement shearing stream generation apparatus etc..On Device is stated to be used widely in correlative study as the experimental rig of vertical shear flow.This kind of device is typically only capable to produce Vertical shear flow is, it is necessary to which the different water body of two kinds of density, device causes disturbance to experiment water body in itself, and water column stability is poor, and And it is unfavorable for circular flow long-term and stably.
The content of the invention
The invention provides a kind of method simple practical, it can fully meet the cyclical level shear flow condition of experiment condition Under microorganism hydrodynamics experimental method.It can realize the horizontal shear hydraulics condition of long-time circular flow, dress Zhi Ge mechanisms hardly produce disturbance to water body, and stability is good, and need not configure the water body of different densities, also will not be in fortune Injury is produced to the microorganism in water body during row.
The technical solution adopted in the present invention is as follows:
A kind of microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow, it is characterised in that the experiment side Method includes:
Using the tank of a loop configuration, Experimental Flowing Object is added in the tank, by the inner cylinder for driving annular water tank The rotation of reverse direction is carried out with outer barrel, the flow velocity and fluidised form of shear flow in tank is formed and change.
In embodiment, the experimental method further comprises:The inner cylinder of the tank is with outer barrel with concentric circles knot Structure is set, and bottom of gullet is using a base as the common support of inner cylinder and outer barrel, and the inner cylinder and outer barrel use transparent material It is made.
In one embodiment, the experimental method further comprises:The correspondence position of inner cylinder and outer barrel is opened on the base Groove is simultaneously provided with dynamic water sealing device, the inner cylinder and outer barrel relative to base external force driving under opposite direction around concentric circles The center of circle is rotated.
In another embodiment, the experimental method further comprises:Circular arc knot is opened up in the lower edge of inner cylinder and outer barrel The groove of structure, makes inner cylinder and outer barrel are in close contact in rotation between dynamic water sealing device and forms sealing.
In one embodiment, the experimental method further comprises:Based on the motor of floor installation two, a driving Motor drives inner cylinder, and another motor drives outer barrel.
In a further embodiment, the motor is set up by support and fixed, and motor is by leading power plate driving company Connect the inner cylinder and outer barrel.
In one embodiment, the experimental method further comprises:
By controlling the velocity of rotation of the inner cylinder and outer barrel to carry out the flow velocity of shear flow and the adjustment of fluidised form in tank;Will Laser film source level is injected in the annular water tank, and is measured and capture using CCD camera.
The present invention produces cyclical level shear flow by a kind of special experimental method, and the shear flow is in annular water tank The continual and steady shear flow that thimble face is produced, cutting in annular water tank can be changed by the velocity of rotation for adjusting tank inside and outside wall Cut the flow velocity and fluidised form of stream.When carrying out microorganism hydrodynamics, Experimental Flowing Object and microorganism are added in annular water tank, is started Drive device inner cylinder and outer barrel are turned to and rotating speed control, you can the water body environment of the eastern water body of simulation true to nature, reach Optimal experiment effect and data.
In order to realize leakage-preventing effect of the water body in the case where inner cylinder and outer barrel are rotated, experimental method of the invention in annular water tank Employ the inner cylinder and outer barrel of tubular structure, and with inner cylinder, outer barrel coordinate the base of bottom movable sealing.Concrete application In, inner cylinder and outer barrel can use clear glass or glass fibre reinforced plastic structure, base using steel plate making, offered on base with inner cylinder and The corresponding groove of outer barrel, is provided with dynamic water sealing device in groove, and the dynamic water sealing device can use polymer sealant Bar.The lower edge of inner cylinder and outer barrel is designed as arcuate structure, in order to friction of the reduction with moving water sealing device part when rotating Power and be in close contact form water-stop.Inner cylinder and outer barrel are designed using concentric structure, and its rotation can be installed using on base Fixed motor is driven, and the erection that motor carries out inner cylinder and outer barrel home position by support is fixed, two Motor carries out being connected for radial direction between inner cylinder and outer barrel by leading power plate respectively.
The beneficial effects of the present invention are the microorganism hydrodynamics experimental method letter under the conditions of the cyclical level shear flow Single practical, can fully meeting experiment condition, it can realize the horizontal shear hydraulics condition of long-time circular flow, dress Zhi Ge mechanisms hardly produce disturbance to water body, and stability is good, and need not configure the water body of different densities, also will not be in fortune Injury is produced to the microorganism in water body during row.
The present invention is further elaborated with reference to the accompanying drawings and detailed description.
Brief description of the drawings
Fig. 1 is a state simulation structural representation of the specific embodiment of the invention;
Fig. 2 is the experimental provision structural representation in the specific embodiment of the invention;
Fig. 3 is the cut-away section structural representation of the experimental provision in the specific embodiment of the invention.
Embodiment
As shown in figure 1, the microorganism hydrodynamics experimental method system under the conditions of the cyclical level shear flow is using an annular The tank of structure, Experimental Flowing Object is added in the tank, by driving the inner cylinder 20 and outer barrel 10 of annular water tank to carry out inverse side To rotation, form and change the flow velocity and fluidised form of shear flow in tank.Can by the velocity of rotation for adjusting barrel inside and outside tank The flow velocity and fluidised form of shear flow in change annular water tank.When carrying out microorganism hydrodynamics, added in annular water tank real Test fluid and microorganism, start drive device inner cylinder and outer barrel are turned to and rotating speed control, you can simulation true to nature is eastern The water body environment of water body, reaches optimal experiment effect and data.
As shown in Figures 2 and 3, the inner cylinder 20 and outer barrel 10 of tank are columnar structured, and inner cylinder 20 is with outer barrel 10 with concentric Circle structure setting, can be made of the transparent material of glass or glass steel material etc., laser film source level is injected into the annular After in tank, usable CCD camera is measured and capture.Bottom of gullet is used as inner cylinder 20 and outer barrel 10 using a base 30 Common support and sealed bottom, the base 30 can be made of steel plate.Arc-shaped structure is opened up in the lower edge of inner cylinder and outer barrel The correspondence position of inner cylinder and outer barrel is slotted and is provided with dynamic water sealing device (not shown) on groove 31, base, while The lower edge of inner cylinder and outer barrel opens up the groove of arc-shaped structure, makes inner cylinder and outer barrel close between dynamic water sealing device in rotation Contact forms sealing.The opposite direction under external force driving is rotated around the center of circle of concentric circles relative to base for inner cylinder 20 and outer barrel 10. The external force is driven to two motors 11 and 21 installed based on the base 30, and a motor 21 drives inner cylinder 20, separately One motor 11 drives outer barrel 10.Motor 11,21 is set up by support 13 and 23 fix respectively, motor 11,21 Respectively by leading power plate 12, outer barrel 10 and inner cylinder 20 described in 22 drive connections.Motor is respectively positioned on the axis of inner cylinder and outer barrel On line, the velocity of rotation that can control the inner cylinder and outer barrel by motor carries out the flow velocity of shear flow and fluidised form in tank Adjustment.

Claims (7)

1. a kind of microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow, it is characterised in that the experimental method Including:
Using the tank of a loop configuration, Experimental Flowing Object is added in the tank, by driving the inner cylinder of annular water tank and outer Cylinder carries out the rotation of reverse direction, forms and change the flow velocity and fluidised form of shear flow in tank.
2. the microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow according to claim 1, its feature It is that the experimental method further comprises:The inner cylinder of the tank is set with outer barrel with concentric structure, and bottom of gullet is used One base is as the common support of inner cylinder and outer barrel, and the inner cylinder and outer barrel are made of transparent material.
3. the microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow according to claim 2, its feature It is that the experimental method further comprises:The correspondence position of inner cylinder and outer barrel is slotted and is provided with dynamic water-stop on the base Device, the opposite direction under external force driving is rotated around the center of circle of concentric circles relative to base for the inner cylinder and outer barrel.
4. the microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow according to claim 3, its feature It is that the experimental method further comprises:The groove of arc-shaped structure is opened up in the lower edge of inner cylinder and outer barrel, makes inner cylinder and outer Cylinder is in close contact between dynamic water sealing device when rotating and forms sealing.
5. the microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow according to claim 2, its feature It is that the experimental method further comprises:Based on the motor of floor installation two, motor driving inner cylinder is another Motor drives outer barrel.
6. the microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow according to claim 5, its feature It is that the motor is set up by support to fix, motor is by leading inner cylinder and outer barrel described in power plate drive connection.
7. the microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow according to claim 1, its feature It is that the experimental method further comprises:
By controlling the velocity of rotation of the inner cylinder and outer barrel to carry out the flow velocity of shear flow and the adjustment of fluidised form in tank;By laser Film source level is injected in the annular water tank, and is measured and capture using CCD camera.
CN201710341977.3A 2017-05-15 2017-05-15 Microorganism hydrodynamics experimental method under the conditions of cyclical level shear flow Pending CN107012196A (en)

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Cited By (3)

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Publication number Priority date Publication date Assignee Title
CN109323844A (en) * 2018-09-30 2019-02-12 河海大学 The annular water tank device of density stratification water body in a kind of simulation shear flow
CN113324731A (en) * 2021-05-17 2021-08-31 哈尔滨工程大学 Experimental device for be used for research of underwater shear flow field electric spark bubble
CN115656478A (en) * 2022-11-14 2023-01-31 中国科学院、水利部成都山地灾害与环境研究所 Seepage-proofing shearing test device for simulating ice particle circulating shearing and using method

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109323844A (en) * 2018-09-30 2019-02-12 河海大学 The annular water tank device of density stratification water body in a kind of simulation shear flow
CN109323844B (en) * 2018-09-30 2019-12-31 河海大学 Annular water tank device for simulating shear flow medium-density layered water body
CN113324731A (en) * 2021-05-17 2021-08-31 哈尔滨工程大学 Experimental device for be used for research of underwater shear flow field electric spark bubble
CN115656478A (en) * 2022-11-14 2023-01-31 中国科学院、水利部成都山地灾害与环境研究所 Seepage-proofing shearing test device for simulating ice particle circulating shearing and using method

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