CN107012083B - Sample treatment combined box for clinical instant detector - Google Patents
Sample treatment combined box for clinical instant detector Download PDFInfo
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- CN107012083B CN107012083B CN201710372542.5A CN201710372542A CN107012083B CN 107012083 B CN107012083 B CN 107012083B CN 201710372542 A CN201710372542 A CN 201710372542A CN 107012083 B CN107012083 B CN 107012083B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The invention provides a sample treatment combined box for a clinical instant detector, which comprises a sliding part, a driving device for driving the sliding part to move, a communicating pipe arranged on the sliding part, and a plurality of saccular containers arranged on the moving track of the sliding part, wherein the saccular containers are provided with openings and are tightly contacted with the sliding part, any two saccular containers are in an unconnected state when the communicating pipe is in a wheel-empty position, the sliding part is driven by the driving device to move, the communicating pipe sequentially communicates different saccular containers along with the movement of the sliding part, and when the two saccular containers are subjected to external force, the two saccular containers are subjected to pressure difference, and liquid exchange can be carried out between the two saccular containers. Compared with the prior art that a structure for controlling the flow of the trace liquid is completed by using more than ten valves, the invention is simpler and more practical, and the manufacturing cost is greatly reduced, thereby effectively reducing the consumable cost.
Description
Technical Field
The invention relates to the field of molecular diagnosis, in particular to a sample treatment combined box for a clinical instant detector.
Background
Techniques for detecting changes in the structure or expression levels of genetic material in a patient using molecular biological methods to make clinical point-of-care diagnoses are referred to as molecular point-of-care diagnoses. The genetic material includes DNA, RNA, etc. of the human body, or of other pathogens. The diagnosis technique mainly uses the complementary pairing hybridization property of nucleic acid, and the polymerase chain reaction (Polymerase Chain Reaction, PCR) copy amplification property extended according to the complementary pairing hybridization property is composed of 1) nucleic acid sample collection and preparation, 2) real-time quantitative polymerase chain reaction, 3) PCR quantitative hybridization technique such as fluorescence, and 4) data analysis.
The ideal instrument for realizing the clinical instant diagnosis technology can realize the full-automatic, high-flux and full-closed nucleic acid extraction, nucleic acid amplification, real-time PCR detection, nested PCR multipoint detection, high-resolution melting (HRM) detection and multiple hybridization detection.
There are a number of instruments available on the market for molecular diagnostics according to the principles described above.
For example, the molecular clinical instant diagnostic apparatus developed by Idaho Technology, inc. In the united states, contains a FilmArray, which is a disposable sample processing kit, and contains a complex multi-valve fluid control device. The diagnosis combination box needs to adopt more than ten valves to complete the transfer of trace liquid among a plurality of containers, so that the diagnosis combination box has complex instruments and high manufacturing cost.
Disclosure of Invention
The invention provides a sample processing combination box on a clinical instant detector, which adopts a sliding multi-valve to control the liquid flow, can realize the sequential control of the operation of a plurality of valves by using a simpler mechanical structure, realizes the transfer of trace liquid, nucleic acid and the like among a plurality of containers, effectively simplifies the mechanical structure and reduces the cost.
The utility model provides a sample processing combination box for on clinical instant detector, includes the slider, drives the drive arrangement that the slider removed, locate the communicating pipe of slider, locate a plurality of saccular containers on the slider moving track, a plurality of saccular containers have the opening and with slider in close contact with two arbitrary saccular containers are in the non-intercommunication state when communicating pipe is in the empty position of wheel, the slider is in the drive arrangement moves down, communicating pipe is with different saccular containers intercommunication in proper order along with the removal of slider, when two saccular containers of intercommunication receive the exogenic action to make there is pressure differential between two saccular containers, can carry out liquid exchange between two saccular containers of intercommunication.
Further, the sliding part is a circular rotating part, the circular rotating part is driven by the driving device to do autorotation, the communicating pipe is arranged in the circular rotating part, an opening of the communicating pipe is arranged on the outer side wall of the circular rotating part, and the plurality of saccular containers are arranged around the circumferential outer wall of the circular rotating part.
Further, the sliding piece is a rod-shaped sliding piece, the plurality of saccular containers are arranged on the side edges of the rod-shaped sliding piece, and the rod-shaped sliding piece is driven by the driving device to do linear motion along the length direction of the rod-shaped sliding piece.
Further, a controllable magnetic device is arranged on the sliding part near the communicating pipe, when the controllable magnetic device has a magnetic field, the communicating pipe can absorb and contain the magnetic beads, along with the sliding of the sliding part, the nucleic acid absorbed on the magnetic beads in the communicating pipe is transported together with the magnetic beads, and when the magnetic field disappears, the magnetic beads can fall off from the communicating pipe.
Furthermore, glass wool is arranged in the communicating pipe of the sliding part, nucleic acid adsorbed on the glass wool is transported along with the sliding of the sliding part, and the glass wool can adsorb nucleic acid dissolved in water by utilizing capillary effect, so that the adsorbed nucleic acid is transported along with the sliding of the sliding part.
Further, the driving device is a digital control motor.
Furthermore, the communicating pipe is of a U-shaped structure.
Further, the plurality of bladder containers can each generate different pressures within the bladder container under the influence of external forces.
The invention provides a novel sample processing combination box for a clinical instant detector, which is characterized in that the novel sample processing combination box is constructed by adopting a sliding multi-valve to control liquid flow, a communicating pipe on a sliding part to transport nucleic acid and a plastic high-elastic material to seal the processing box on the sample processing box. Compared with the prior art, the novel sample treatment combined box has the advantages that more than ten valves are used for controlling the flow of the trace liquid, the novel sample treatment combined box is simpler and more practical, and the manufacturing cost is greatly reduced, so that the consumable cost of a user is effectively reduced.
Drawings
FIG. 1 is a schematic view of a communication pipe according to a first embodiment of the present invention;
fig. 2 is a schematic structural diagram of a communication pipe according to the first embodiment of the present invention;
fig. 3 is a schematic structural diagram of a communication pipe according to the second embodiment of the present invention when the communication pipe is not connected;
fig. 4 is a schematic structural diagram of a communication pipe in the second embodiment of the present invention;
FIG. 5 is a schematic illustration of a third embodiment of the invention with magnetic beads residing in a capsule container;
FIG. 6 is a schematic view showing a third embodiment of the present invention in which magnetic beads are adsorbed on a communication tube;
FIG. 7 is a schematic diagram showing adsorption of nucleic acid by glass wool in a communicating tube according to a fourth embodiment of the present invention;
FIG. 8 is a schematic diagram showing nucleic acid capillary-adsorbed on glass wool being transported on a four-way communication tube according to an embodiment of the present invention;
in the figure: 101. 102, 115, 116, 512, 513, 514, 515-a capsule, 103, 113, 511-a communicating tube, 104-a circular rotating member, 114-a rod-shaped sliding member, 117-magnetic beads, 118-a controllable magnetic device.
Detailed Description
The method for constructing the novel sample processing combination box by controlling the liquid flow by the sliding multi-valve adopted by the technical scheme of the invention, transferring the nucleic acid by the communicating pipe on the sliding part and sealing the processing box by the plastic high-elastic material is clearly and completely described below with reference to the attached drawings.
Biological experiments have in many cases involved the transfer of trace amounts of liquid between two containers. The invention adopts a sample processing combined box for a clinical instant detector, and can sequentially control the operation of a plurality of valves by only one mechanical action, such as rotation or horizontal movement. One such control device can replace the function of multiple valves, allowing the entire instrument to run freely.
The invention provides two sliding multi-valve control device embodiments, namely: a sample processing combination box for a clinical instant detector by adopting a rotary sliding communication valve or a sample processing combination box for a clinical instant detector by adopting a rod-shaped sliding communication valve.
Referring to fig. 1 and 2, a first embodiment of the present invention includes a circular rotating member 104 and a plurality of bladder containers disposed around the outer wall of the circumference of the circular rotating member 104, wherein all of the bladder containers can generate different pressures in the bladder containers under the action of external force. The circular rotating member 104 may be driven by a driving device (e.g., a digital control motor) to perform rotation, a communicating pipe 103 is disposed in the circular rotating member 104, and an opening of the communicating pipe 103 is disposed on an outer sidewall of the circular rotating member 104. When the circular rotation member 104 is rotated to a certain position, the communicating tube 103 may communicate two bladder containers (101, 102) among the plurality of bladder containers while the other bladder containers are in an unconnected state.
As shown in fig. 1, the circular rotating member 104 may have a disc-shaped structure, a plurality of capsule-shaped containers are enclosed around the outer wall thereof and closely contact with the outer wall, the communicating tube 103 may have a U-shaped structure, and openings at both ends thereof may be butted with openings at the upper ends of the capsule-shaped containers. When the communication pipe 103 of the circular rotation member 104 is in the wheelspace position, the liquid in the bladder container cannot flow through the communication pipe 103 at all. When the circular rotation member 104 rotates by a certain position, as shown in fig. 2, the communicating pipe 103 is positioned at a position where the adjacent bladder containers (101, 102) are communicated, and in the case where there is a pressure difference between the two bladder containers (101, 102), the liquid can flow between the adjacent bladder containers (101, 102) through the communicating pipe 103. The circular rotating member 104 continues to rotate, the communicating tube 103 moves to the next position, and the communicating tube 103 can sequentially connect different saccular containers, thereby achieving the purpose of controlling the flow direction of liquid.
Referring to fig. 3 and 4, the embodiment of the present invention includes a rod-shaped sliding member 114 and a plurality of capsule-shaped containers closely contacted with the rod-shaped sliding member 114, wherein all capsule-shaped containers can generate different pressures in the capsule-shaped containers under the action of external force. The rod-shaped sliding part 114 can be driven by a driving device (such as a numerical control motor) to do linear motion along the length direction of the rod-shaped sliding part, a communicating pipe 113 is arranged on the outer side wall of the rod-shaped sliding part 114, when the rod-shaped sliding part 114 slides to a certain position, the communicating pipe 113 can communicate two saccular containers (115 and 116) in the plurality of saccular containers, and other saccular containers are in an unconnected state at the moment.
As shown in fig. 3, the rod-shaped sliding member 114 may have a cross bar structure, a plurality of capsule-shaped containers are disposed on the side of the rod-shaped sliding member 114 (e.g., above or below along the length direction of the cross bar) and are in close contact with the outer wall of the rod-shaped sliding member 114, the communicating tube 113 may have a U-shaped structure, and openings at both ends thereof may be in butt joint with openings at the upper ends of the capsule-shaped containers. When the communicating tube 113 of the rod-shaped slider 114 is in the wheelspace position, the liquid in the capsule-shaped container cannot flow through the communicating tube 113 at all. When the rod-shaped slider 114 moves to a certain position, as shown in fig. 4, the communicating pipe 113 is positioned to communicate the adjacent bladder containers (115, 116), and when there is a pressure difference between the two bladder containers (115, 116), the liquid can flow between the adjacent bladder containers (115, 116) through the communicating pipe 113. The rod-shaped sliding member 114 continues to rotate, the communicating pipe 113 moves to the next position, and the communicating pipe 113 can sequentially connect different saccular containers, so that the purpose of controlling the flow direction of liquid is achieved.
The communicating tube used in the present invention has another application in which nucleic acid adsorbing materials such as: siliconized magnetic beads, solid silica fibers (e.g., glass wool), adsorbed or disposed within the communicating tube, transport nucleic acids attached to the solid silica fibers or the siliconized magnetic beads, or with nucleic acids dissolved in a liquid.
In the third embodiment, referring to fig. 5 and 6, the communicating tube 113 is used for transporting nucleic acid adsorbing material and then transporting nucleic acid. The capsule 115 or 117 in this embodiment contains nucleic acid, nucleic acid lysate, and nucleic acid adsorbing material (e.g., siliconized magnetic beads 116). A controllable magnetic device 118 is provided on the rod-shaped slider 114 near the communication tube 113, which can control the presence or absence of magnetic force by turning on/off the power, thereby realizing convenient transfer of the adsorbed minute amount of nucleic acid. Specifically, the nucleic acid is dissolved in the saccular container 115 or the saccular container 117 by the nucleic acid lysate. By the force of the outside of the capsule, alternating and repeated positive and negative pressures are generated in the capsule, so that the liquid repeatedly flows between the two capsules (figure 5). After adsorbing nucleic acids, the magnetic beads 116 in the nucleic acid-containing nucleic acid lysate are magnetically collected in the communication tube 113 of the rod-shaped slide 114 (as shown in FIG. 6), and the adsorbed nucleic acids move with the rod-shaped slide.
Referring to fig. 7 and 8, a fourth embodiment of the invention is a schematic diagram of a communicating tube 511 for transporting nucleic acid dissolved in a liquid. As shown in fig. 8, in the present embodiment, the communicating tube 511 is a U-shaped tube, glass wool is placed in the communicating tube 511, and when it communicates the saccular container 512 and the saccular container 513, a nucleic acid-containing lysate repeatedly flows between the saccular container 512 and the saccular container 513 through the communicating tube 511, and nucleic acid is adsorbed on the glass wool. In this case, the nucleic acid adsorbed to the glass wool is dehydrated and moves along with the movement of the communicating tube 511. The nucleic acid in the communication tube 511 is further washed between the bladder container 512 and the bladder container 514, and between the bladder container 515 and the bladder container 516.
When the communicating tube 511 communicates the capsule container 517 and the capsule container 518, the eluting solution in the capsule container 517 elutes the nucleic acid on the glass wool, and dissolves it into the eluting solution. Because of the capillary effect of glass wool, the nucleic acid dissolved in the nucleic acid eluent remains adsorbed to the glass wool and can be transported to communicate with the rotation of the communication tube 103 and enter the lower capsule until all the desired reactions are completed.
The invention provides a novel sample processing combination box for a clinical instant detector, which is characterized in that the novel sample processing combination box is constructed by adopting a sliding multi-valve to control liquid flow, a communicating pipe on a sliding part to transport nucleic acid and a plastic high-elastic material to seal the processing box on the sample processing box. Compared with the prior art, the novel sample treatment combined box has the advantages that more than ten valves are used for controlling the flow of the trace liquid, the novel sample treatment combined box is simpler and more practical, and the manufacturing cost is greatly reduced, so that the consumable cost of a user is effectively reduced.
The foregoing is merely illustrative embodiments of the present invention, and the present invention is not limited thereto, and any changes or substitutions that may be easily contemplated by those skilled in the art within the scope of the present invention should be included in the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.
Claims (5)
1. A sample processing combination box for a clinical instant detector, which is characterized in that: the device comprises a sliding part, a driving device for driving the sliding part to move, a communicating pipe arranged on the sliding part, and a plurality of saccular containers arranged on the moving track of the sliding part, wherein the saccular containers are provided with openings and are in close contact with the sliding part, any two saccular containers are in an unconnected state when the communicating pipe is in a wheel-empty position, the sliding part is driven by the driving device to move, the communicating pipe sequentially communicates different saccular containers along with the movement of the sliding part, and when the two saccular containers are subjected to external force so that pressure difference exists between the two saccular containers, liquid exchange can be carried out between the two saccular containers; the sliding part is a circular rotating part, the circular rotating part is driven by the driving device to do autorotation movement, the communicating pipe is arranged in the circular rotating part, an opening of the communicating pipe is arranged on the outer side wall of the circular rotating part, and the plurality of saccular containers are arranged around the circumferential outer wall of the circular rotating part; or the sliding piece is a rod-shaped sliding piece, the plurality of saccular containers are arranged on the side face of the rod-shaped sliding piece, and the rod-shaped sliding piece is driven by the driving device to do linear motion along the length direction of the rod-shaped sliding piece; the magnetic device is characterized in that a controllable magnetic device is arranged outside the communicating pipe on the sliding part, when the controllable magnetic device has a magnetic field, the communicating pipe can absorb and contain magnetic beads, nucleic acid absorbed on the magnetic beads in the communicating pipe is transported together with the magnetic beads along with the sliding of the sliding part, and when the magnetic field disappears, the magnetic beads can fall off from the communicating pipe.
2. The sample processing combination cassette for use on a clinical point-of-care meter according to claim 1, wherein: the plurality of bladder containers can generate different pressures in the bladder containers under the action of external force.
3. The sample processing combination cassette for use on a clinical point-of-care meter according to claim 1, wherein: the communicating pipe is provided with solid silicon fibers, nucleic acid adsorbed on the solid silicon fibers is transported along with the sliding of the sliding piece, and the solid silicon fibers can adsorb nucleic acid dissolved in water by utilizing capillary effect, and the adsorbed nucleic acid is transported along with the sliding of the sliding piece.
4. A sample processing kit for use on a clinical point-of-care meter according to any one of claims 1-3, wherein: the driving device is a numerical control motor.
5. A sample processing kit for use on a clinical point-of-care meter according to any one of claims 1-3, wherein: the communicating pipe is of a U-shaped structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710372542.5A CN107012083B (en) | 2017-05-24 | 2017-05-24 | Sample treatment combined box for clinical instant detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710372542.5A CN107012083B (en) | 2017-05-24 | 2017-05-24 | Sample treatment combined box for clinical instant detector |
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| Publication Number | Publication Date |
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| CN107012083A CN107012083A (en) | 2017-08-04 |
| CN107012083B true CN107012083B (en) | 2023-10-17 |
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| CN201710372542.5A Active CN107012083B (en) | 2017-05-24 | 2017-05-24 | Sample treatment combined box for clinical instant detector |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1646222A (en) * | 2002-02-25 | 2005-07-27 | 西菲伊德公司 | Fluid processing and control |
| CN103403545A (en) * | 2010-12-09 | 2013-11-20 | 阿科尼生物系统公司 | Sample analysis system |
| WO2016117726A1 (en) * | 2015-01-23 | 2016-07-28 | Infopia Co., Ltd. | Cartridge |
| CN206736264U (en) * | 2017-05-24 | 2017-12-12 | 吴春梅 | A kind of sample treatment combination box on clinical instant detector |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11260386B2 (en) * | 2015-06-05 | 2022-03-01 | The Emerther Company | Component of a device, a device, and a method for purifying and testing biomolecules from biological samples |
-
2017
- 2017-05-24 CN CN201710372542.5A patent/CN107012083B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1646222A (en) * | 2002-02-25 | 2005-07-27 | 西菲伊德公司 | Fluid processing and control |
| CN103403545A (en) * | 2010-12-09 | 2013-11-20 | 阿科尼生物系统公司 | Sample analysis system |
| WO2016117726A1 (en) * | 2015-01-23 | 2016-07-28 | Infopia Co., Ltd. | Cartridge |
| CN206736264U (en) * | 2017-05-24 | 2017-12-12 | 吴春梅 | A kind of sample treatment combination box on clinical instant detector |
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| Publication number | Publication date |
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| CN107012083A (en) | 2017-08-04 |
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Effective date of registration: 20230918 Address after: 361000 No.188, Pingcheng South Road, Haicang street, Haicang District, Xiamen City, Fujian Province (3rd floor) Applicant after: Xiamen Baotai Biotechnology Co.,Ltd. Address before: 361026 floor 3, building 4, Zhongcang Industrial Park, No. 19, Pingcheng Middle Road, Haicang District, Xiamen City, Fujian Province Applicant before: Wu Chunmei |
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