CN107011451B - The different poly- polysaccharide of tremella and its extract, preparation method and application - Google Patents
The different poly- polysaccharide of tremella and its extract, preparation method and application Download PDFInfo
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- CN107011451B CN107011451B CN201610261524.5A CN201610261524A CN107011451B CN 107011451 B CN107011451 B CN 107011451B CN 201610261524 A CN201610261524 A CN 201610261524A CN 107011451 B CN107011451 B CN 107011451B
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of different poly- polysaccharide of tremella and its extracts, preparation method and application.The preparation method of the different poly- polyoses extract of tremella in the present invention is the following steps are included: acid flocculant is mixed with the tremella alkali extract for being adjusted to faintly acid or neutrality through acid, flocculation is separated by solid-liquid separation to obtain sediment composite；Wherein, the acid flocculant is the aqueous acid of flocculant, and the flocculant is chitosan.The different poly- polysaccharide of tremella that the different molecular weight ranges of high-purity can be made using preparation method of the invention, yield can achieve 20% or more, and purity can achieve 93% or more；10 made from preparation method using the application~the different poly- polysaccharide of tremella of 200,000 dalton shows the good ability facilitated fibrocyte proliferation effect and promote fibroblasts to secrete hyaluronic acid, while also showing excellent percutaneous permeability.
Description
Technical field
The present invention relates to a kind of different poly- polysaccharide of tremella and its extracts, preparation method and application.
Background technique
The different poly- polysaccharide of tremella can be applied to drug, health care product, in foods and cosmetics, tool as a kind of functional polysaccharide
There are many effects.Patent CN100402555C disclose a kind of molecular weight for being extracted under aqueous solution hot conditions 85~
The white fungus heteropolysaccharideand of 1600000 dalton, and disclose its with moisturizing greasy skin and mucous membrane, the sense of enhancing lubrication, anti-oxidant decline
Always, antiallergy, thick stabilization and other effects.It is disclosed in patent CN102161710A different using acid hydrolyzation preparation low molecular weight tremella
The method of poly- polysaccharide, and disclosing molecular weight is the different poly- polysaccharide of 500~10000 dalton tremellas in preparation treatment atrophic
The drug of gastritis and prepare the application in Ancillary drugs in tumor therapy.Aqueous slkali and aqueous solution are disclosed in patent CN1778824A
The different poly- polysaccharide of the homogeneous tremella of extraction, molecular weight in 50,000~70,000 dalton, be respectively provided with inhibit lymthoma, it is anti-radiation,
Strengthen immunity, antiviral, anti-oxidant and other effects.
The effect of can be seen that the different poly- polysaccharide of tremella of (1) different molecular weight by the above existing patented technology is different；
It (2), must when preparing the polysaccharide of low molecular weight since polysaccharide is often formed in the bodies of aminal and plant with biggish molecular state
Need certain method that fracture is hydrolyzed to glycosidic bond.The method of hydrolyzing glucosidic bonds common at present has acidolysis, alkaline hydrolysis and enzyme
Solution.
Acidolysis is hydrolyzed using acidic materials such as certain density hydrochloric acid, and non-specific hydrolysis is belonged to, can be very square
The glycosidic bond of macromolecular polysaccharide being broken just, but acidolysis will also tend to cause the excessive hydrolysis of polysaccharide, on the one hand can not
It realizes being precisely controlled for molecular weight, on the other hand will also result in the decline of polysaccharide yield, influence its technical application value.In addition,
This method has specific requirement to production equipment due to the presence of acid, is unfavorable for the production conversion of product.
Alkaline hydrolysis belongs to non-specific hydrolysis as acidolysis, but its hydrolysis is mild, can pass through different technology conditions
Setting obtain specified molecular weight polysaccharide.In addition, it is of less demanding to production equipment, technique application does not need specifically to set
It is standby.But alkaline hydrolysis, there is also some problems, alkaline hydrolysis technique is easy to cause product colour to deepen, it can also will be in plant material tissue
Cellulose hydrolysis, obtain alkali cellulose sodium and its pyrolysis product, these substances as impurity eventually with desired polysaccharide together
By ethanol precipitation, enter final products as impurity, seriously affects the purity and effect of product.Therefore, lye extraction is being carried out
When different molecular weight polysaccharide, it is necessary to consider removal because of the related impurities that cellulose hydrolyzes and generates, in addition also to solve to produce
The saturate problem of product.
In addition, it is more difficult using the selection of enzyme class when enzyme solution, because of its glycosidic bond links mode of different polysaccharide
It differs greatly, and also tends in same polysaccharide there are a variety of glycosidic bonds, but the specificity of enzyme is again stronger, a kind of enzyme can only
A kind of glycosidic bond is hydrolyzed, so there are larger difficulty for the screening of enzyme, the different microorganisms such as saccharomycete is mostly used to ferment at present
For method to obtain the enzymatic hydrolysis that certain specific enzyme carries out polysaccharide, the development cycle is long, costly；The different poly- polysaccharide macro-molecular structure of tremella
Mainly with α -1, the on-link mode (OLM) of 3 glycosidic bonds exists, and there is no the enzyme of specificity to can be applied to the different poly- polysaccharide of tremella at present and divide greatly
The enzymatic hydrolysis of minor structure.
Summary of the invention
The technical problem to be solved by the present invention is in order to overcome, to obtain tremella different poly- more for alkaline hydrolysis glycosidic bond in the prior art
The method purity of sugar is lower, saturate problem, and provides a kind of different poly- polysaccharide of tremella and its extract, preparation method and answer
With.The different poly- polysaccharide of tremella that the high-purity of different molecular weight ranges can be made using preparation method of the invention, yield can reach
To 20% or more, purity can achieve 93% or more；10 made from preparation method using the application~silver of 200,000 dalton
The different poly- polysaccharide of ear shows the good energy facilitated fibrocyte proliferation effect and promote fibroblasts to secrete hyaluronic acid
Power, while also showing excellent percutaneous permeability.
The present invention provides a kind of preparation methods of the different poly- polyoses extract of tremella comprising following steps: acidity is wadded a quilt with cotton
Solidifying agent is mixed with the tremella alkali extract for being adjusted to faintly acid or neutrality through acid, is flocculated, is separated by solid-liquid separation to obtain sediment composite；Its
In, the acid flocculant is the aqueous acid of flocculant, and the flocculant is chitosan.
Acidity flocculant of the present invention is generally obtained by the way that the chitosan to be dissolved in sour water.Wherein, institute
The sour water stated is generally the conventional sour water that can be dissolved chitosan in this field, the preferred aqueous hydrochloric acid solution of the present invention；Described
The mass concentration of aqueous hydrochloric acid solution preferably 0.25%~1.0%.The chitosan is referred to this field and is routinely selected,
The specification of the preferred chitosan of the present invention are as follows: deacetylation >=85%；And at 20 DEG C, concentration be 10 grams per liter when viscosity >=
200mpa.s.In the acid flocculant, the concentration of the flocculant preferably 0.005~0.02g/mL, more preferably
0.01g/mL。
In the preparation method of the different poly- polyoses extract of tremella of the present invention, it is described " through acid be adjusted to faintly acid or in
Property tremella alkali extract " in sour type can be the type of conventional use of acid in the generic operation of this field, the present invention is preferably dilute
Hydrochloric acid；The mass concentration of the dilute hydrochloric acid preferably 0.5%~6.0%, more preferable 1%~6.0%；The faintly acid is general
Refer to 4≤PH < 7, preferably 6≤PH < 7；The neutrality refers generally to PH=7.The tremella refers generally to Tremella fructification raw material；
The Tremella fructification raw material can be the Tremella fructification raw material for obtaining Tremella fructification raw material using pure water soaking and washing.
It is of the present invention " to be adjusted to weak acid through acid in the preparation method of the different poly- polyoses extract of tremella of the present invention
Property or neutral tremella alkali extract " preparation method is referred to this field and routinely selected, generally are as follows: tremella is subjected to alkaline hydrolysis
After obtaining tremella alkali extract, the tremella alkali extract is adjusted with acid to faintly acid or neutrality, the filtrate being obtained by filtration is institute
" the tremella alkali extract of faintly acid or neutrality is adjusted to through acid " stated.Wherein, the tremella can directly carry out alkaline hydrolysis and obtain " institute
The tremella alkali extract stated " carries out alkaline hydrolysis after can also being homogenized the tremella again and obtains the tremella alkali extract.Described
The preferred decompression essence filtering of filtering.The condition of the alkaline hydrolysis can be selected according to the different poly- polysaccharide of tremella of different molecular weight ranges
It selects, different alkaline hydrolysis conditions (type, concentration and dosage including alkali, the time of alkaline hydrolysis and temperature etc.) available different molecular
The different poly- polysaccharide of tremella of range is measured, the alkaline hydrolysis condition in the present invention is preferably as follows: the preferred sodium hydroxide of alkali；The alkali can be with
The form of aqueous alkali participates in reaction, the mass concentration of the aqueous alkali preferably 1~15% (such as 3%, 5%, 6%,
8% or 10%)；The volume mass of the aqueous alkali and the tremella than preferably 10~30mL/g (such as 15mL/g,
20mL/g or 25mL/g)；The time of the alkaline hydrolysis preferably 2~10h (such as 3h, 4h, 6h or 8h)；The alkaline hydrolysis temperature is excellent
Select 90~98 DEG C.
In the preparation method of the different poly- polyoses extract of tremella of the present invention, the dosage of the acid flocculant is general
For that completely, i.e., the different poly- polysaccharide of tremella in described " being adjusted to the tremella alkali extract of faintly acid or neutrality through acid " can be flocculated
It can；In the present invention, " it is adjusted to the tremella alkali extract of faintly acid or neutrality through acid " described in every 100mL and preferably adds 5~10mL institute
The acid flocculant stated；Acid flocculant described in more preferably 5~8mL of addition.Preferably 10~35 DEG C of the flocculated temperature；
The flocculation preferably carries out under stiring.The routine that the separation of solid and liquid is referred to this field is selected, the present invention
It is preferred that filtering.
In the preparation method of the different poly- polyoses extract of tremella of the present invention, it is described " through acid be adjusted to faintly acid or in
The tremella alkali extract of property " and the hybrid mode of " the acid flocculant " are not particularly limited, generally by the acidity
Flocculant is added in described " being adjusted to the tremella alkali extract of faintly acid or neutrality through acid ".
The preparation method of the different poly- polyoses extract of tremella of the present invention may be used also after obtaining the sediment composite
It further includes steps of and dissociates the sediment composite under alkaline condition, be separated by solid-liquid separation, obtain filtrate A.
In the preparation of the filtrate A, described being dissociated into separates the acid flocculant and the different poly- polysaccharide of tremella
Process, the present invention in preferably include following steps: after the sediment composite is mixed with water, with alkali be adjusted to PH be 8.0
~10.0 (such as 9.0) are dissociated at 50~60 DEG C.Wherein, the preferred pure water of the water, the pure water in this field
Generally deionized water；The routine that the dosage of the water is referred to this field is selected, the water and the precipitating
The volume mass of compound is than preferably 10~20mL/g, more preferable 12~15mL/g.The alkali can make the precipitating compound
Object dissociation, the preferred sodium hydroxide of the application；The sodium hydroxide can be carried out instead in the form of sodium hydrate aqueous solution
It answers, the sodium hydrate aqueous solution that the sodium hydrate aqueous solution preferred mass concentration is 4%~10%, more preferable mass concentration
For 5% sodium hydrate aqueous solution.
The present invention also provides a kind of preparation methods of the different poly- polysaccharide of tremella comprising following steps: by above-mentioned filtrate A
Decoloration, is adjusted to neutrality with acid, is concentrated, alcohol precipitation, collects precipitating.
In the preparation method of the different poly- polysaccharide of the tremella, the routine that the decoloration is referred to this field is selected
It selects, present invention preferably employs hydrogen peroxide to decolourize；Wherein, the peroxide that the hydrogen peroxide preferred mass score is 30%
Change aqueous solution of hydrogen.The dosage of the hydrogen peroxide, which is generally, decolourizes the filtrate A, for example, the quality point
Number is preferably the 0.5%~2.0% of the filtrate A volume for the volume of 30% aqueous hydrogen peroxide solution, more preferably
1.0%.In the preparation method of the different poly- polysaccharide of the tremella, the acid is referred to the conventional of such reaction of this field and carries out
Selection, the preferred dilute hydrochloric acid of the present invention, the mass concentration of the dilute hydrochloric acid preferably 4%~8%, more preferable 6%.The tremella
In the preparation method of different poly- polysaccharide, the concentration condition is referred to the conventional selection of this field, and the present invention preferably depressurizes dense
Contracting；The concentration is preferably 2~3mL/g to the volume mass of concentrate and tremella ratio, and the tremella is the filtrate A
Preparation process used in tremella raw material.The routine that the step of described alcohol precipitation can refer to this field is selected, generally
Alcohol is mixed with to the concentrate, is precipitated；Wherein, the type of the alcohol be referred to this field it is conventional into
Row selection, preferred alcohol of the present invention；The ethyl alcohol carries out the precipitating, the ethyl alcohol generally in the form of its aqueous solution
The concentration of volume percent of aqueous solution preferably 90%~95%；The terminal of the alcohol precipitation is referred to this field and is routinely selected
It selects, the present invention preferably reaches 60%~70% to the concentration of volume percent of final ethyl alcohol.
The preparation method of the different poly- polysaccharide of tremella of the present invention may also include the step of drying precipitating.Described
Dry preferred vacuum decompression is dry.
In the preparation method of the different poly- polysaccharide of tremella of the present invention, when molecular weight is prepared less than 100,000 dalton
When the different poly- polysaccharide of (such as 5.6 ten thousand, 8.7 ten thousand or 9.6 ten thousand dalton) tremella, tremella is prepared in the preparation process of the filtrate A
The alkali carries condition of sub- alkali extract is preferred are as follows: alkali is sodium hydrate aqueous solution (such as 10% hydrogen that mass concentration is 9%~11%
Aqueous solution of sodium oxide)；The volume mass ratio of the alkali and the tremella is 15~25mL/g (such as 20mL/g)；Alkaline hydrolysis
Temperature is 90~98 DEG C；The time of alkaline hydrolysis is 9~11h (such as 10h), and the molecular weight has been prepared less than 100,000 dongles
Pause the different poly- polysaccharide of tremella.
In the preparation method of the different poly- polysaccharide of tremella of the present invention, it is less than or equal to when molecular weight is prepared greater than 200,000
When the different poly- polysaccharide of 1000000 dalton (such as 22.4 ten thousand, 67.5 ten thousand or 97.1 ten thousand dalton) tremella, the preparation of the filtrate A
The alkali carries condition for preparing the sub- alkali extract of tremella in the process is preferred are as follows: alkali is the sodium hydroxide water that mass concentration is 0.5%~4.5%
Solution (such as 3% sodium hydrate aqueous solution)；The volume mass ratio of the alkali and the tremella is 5~15mL/g (example
Such as 10mL/g)；The temperature of alkaline hydrolysis is 90~98 DEG C；The time of alkaline hydrolysis is 3~6h (preferably 4h), and the molecular weight is prepared
It is less than or equal to the different poly- polysaccharide of 1,000,000 dalton tremellas greater than 200,000.
In the preparation method of the different poly- polysaccharide of tremella of the present invention, when molecular weight 10~200,000 dalton are prepared
When the different poly- polysaccharide of (such as 16.7 ten thousand, 18.5 ten thousand and 12.8 ten thousand dalton) tremella, silver is prepared in the preparation process of the filtrate A
The alkali carries condition of ear alkali extract is preferred are as follows: alkali is sodium hydrate aqueous solution (such as 6% hydroxide that mass concentration is 5%~8%
Sodium water solution)；The volume mass ratio of the alkali and the tremella is 15~25mL/g (such as 20mL/g)；The alkaline hydrolysis
Temperature be 90~98 DEG C；The time of the alkaline hydrolysis is 3~6h (such as 4h), and the molecular weight 10~20 has been prepared
The different poly- polysaccharide of ten thousand dalton tremellas.
It is 10~200,000 dalton the present invention also provides a kind of molecular weight being prepared by above-mentioned preparation method
The different poly- polysaccharide of tremella.The molecular weight is that the molar ratio of the neutral sugar in the different poly- polysaccharide of tremella of 10~200,000 dalton is preferred
For fucose: xylose: mannose: glucose=0.40~0.45 (such as 0.42): 0.34~0.36 (such as 0.35): 1:
0.17~0.25 (such as 0.20)；The molecular weight is glucuronic acid in the different poly- polysaccharide of tremella of 10~200,000 dalton
Mass content preferably 17%~20% (such as 18%).
The present invention also provides a kind of different poly- polysaccharide of tremella of 10~200,000 dalton of molecular weight in preparing skin care item
Using.The effect of described skin care item may include deep water supplement and/or anti-aging.The type of the skin care item does not have special limit
It is fixed, it can be cosmetics or personal-care supplies etc..In addition, the tremella of 10~200,000 dalton of molecular weight of the present invention is different poly-
Polysaccharide can also be used in the fields such as daily chemical product, health food and drug.
The concentration of hydrochloric acid in the present invention refers to the mass fraction of aqueous hydrochloric acid solution unless otherwise specified；Such as 1% hydrochloric acid
Refer to that mass fraction is 1% aqueous hydrochloric acid solution.
The concentration of sodium hydroxide in the present invention refers to the mass fraction of sodium hydrate aqueous solution unless otherwise specified；Such as
10% sodium hydroxide refers to that mass fraction is 10% sodium hydrate aqueous solution.
Room temperature of the present invention refers generally to 10~35 DEG C.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can any combination to get the present invention it is each preferably
Example.
The reagents and materials used in the present invention are commercially available.
The positive effect of the present invention is that:
1. in the present invention in the preparation method of the different poly- polysaccharide of tremella, for some problems existing during alkaline hydrolysis, according to
The design feature of the different poly- polysaccharide of tremella, innovative has used acid flocculant, passes through the network of the different poly- polysaccharide of flocculant and tremella
Precipitating is closed, the different poly- polysaccharide of tremella can be made to be separated with other impurities (such as product of cellulose alkaline hydrolysis), can finally be made high
The different poly- polysaccharide of the low molecular weight tremella of purity can achieve using the yield that preparation method of the invention prepares the different poly- polysaccharide of tremella
20% or more, purity can achieve 93% or more.
2. the application is different poly- using the tremella that the preparation method of the different poly- polysaccharide of above-mentioned tremella realizes different molecular weight ranges
The preparation of polysaccharide, such as the different poly- polysaccharide of tremella of 10~200,000 dalton.
3. the different poly- polysaccharide of tremella of 10~200,000 dalton in the application shows and good fibrocyte is facilitated to increase
It grows effect and promotes the ability of fibroblasts to secrete hyaluronic acid, while also showing excellent percutaneous permeability, it can be with
Increase the effect of skin skin corium storage capacity, there is deep water supplement and/or anti-aging function.
Specific embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to the reality
It applies among a range.In the following examples, the experimental methods for specific conditions are not specified, according to conventional methods and conditions, or according to quotient
The selection of product specification.
Room temperature is generally 10~35 DEG C in specific embodiment.
Acid flocculant solution in specific embodiment, which refers to, to be dissolved the chitosan in the aqueous hydrochloric acid solution of 1% (v/v)
It is prepared；For example, 1% acid flocculant solution refers to that the hydrochloric acid that 1g chitosan is dissolved in 100mL 1% (v/v) is water-soluble
It is prepared in liquid.(wherein, chitosan specification: deacetylation >=85%；Viscosity (10 grams per liters, 20 DEG C) >=200mpa.s)
The method of determination of polysaccharide in specific embodiment and the method for molecular weight determination are the side of this field routine
Method, wherein determination of polysaccharide reference: " saccharide complex biochemical investigations technique ", publishing house, Zhang Weijie Zhejiang University, 1999,
Phend-sulphuric acid in the quantitative determination of chapter 2 2.1 section sugar of page 11；Molecular weight determination reference: it is more that HPLC method measures fungi
The research of sugared average molecular weight method, Kong Xianghui etc., 2006 Annual Conference of the association for science and technology, Heilongjiang Province and the Sun Island science and technology forum,
2006。
Embodiment 1
Take Tremella fructification raw material, pure water soaking and washing；The dense of raw material weight (g) 20 times of volumes is added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that degree is 6%, alkaline hydrolysis extracts 4h under the conditions of 90 DEG C~98 DEG C；After alkaline hydrolysis, with 6% concentration
Dilute hydrochloric acid adjust material liquid PH value to 6.0~7.0；The filtering of conventional vacuum essence is carried out, filtrate is collected；It is added in every 100ml filtrate
The acid flocculant solution that 8ml concentration is 1%, stirring makes it sufficiently flocculate under normal temperature condition, forms a kind of sediment composite；It crosses
Sediment composite is collected in filter；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in raw material weight (g)
20 times of volumes pure water solution (mL) in, and with 5% sodium hydroxide solution adjust material liquid PH value to alkalinity 9.0~10.0,
Dissociation is heated under the conditions of 50 DEG C~60 DEG C.Above-mentioned feed liquid is filtered, filtrate is collected；After sediment progress harmless treatment, abandon
It goes.30% hydrogen peroxide that 1.0% (V/V) of filtrate total volume is added into above-mentioned filtrate carries out decolorization；Expect after decoloration
It is neutral that the dilute hydrochloric acid that liquid is again 6% with mass concentration, which adjusts pH value,；Above-mentioned feed liquid is concentrated under reduced pressure into the 2 of raw material weight (g)
Times~3 times of volumes (mL)；Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, makes ethyl alcohol in final feed liquid
Concentration of volume percent reach 60%~70%, collect precipitating, vacuum decompression is dry to get different poly- more to high-purity tremella
Sugar, yield are as follows: 27.2%；The different poly- polyoses content of tremella are as follows: 95%；It is 16.7 ten thousand dalton through HPLC method measurement molecular weight.
Embodiment 2:
Take Tremella fructification raw material, pure water soaking and washing；The dense of raw material weight (g) 25 times of volumes is added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that degree is 5%, alkaline hydrolysis extracts 3h under the conditions of 90 DEG C~98 DEG C, dense with quality after alkaline hydrolysis
The dilute hydrochloric acid of degree 6% adjusts material liquid PH value to 6.0~7.0；Conventional essence filtering is carried out, filtrate is collected；It is added in every 100ml filtrate
The acid flocculant solution that 5ml concentration is 2%, stirring makes it sufficiently flocculate under normal temperature condition, forms a kind of sediment composite；It crosses
Sediment composite is collected in filter；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in raw material weight (g)
In the pure water solution of 15 times of volumes (mL), and material liquid PH value is adjusted to alkalinity 8.0~9.0,50 with 5% sodium hydroxide solution
DEG C~60 DEG C under the conditions of heat dissociation.Above-mentioned feed liquid is filtered, filtrate is collected；After sediment progress harmless treatment, discard.To
30% hydrogen peroxide that 0.5% (V/V) of filtrate total volume is added in above-mentioned filtrate carries out decolorization；Feed liquid is again after decoloration
It is neutral that the dilute hydrochloric acid for being 6% with mass concentration, which adjusts pH value,；Above-mentioned feed liquid is concentrated under reduced pressure into 2~3 times of bodies of raw material weight (g)
Product (mL)；Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, keeps the precipitating of ethyl alcohol in final feed liquid dense
Degree reaches 60%~70%, collects precipitating, and vacuum decompression is dry to get arriving the different poly- polysaccharide of high-purity tremella, yield are as follows:
29.1%；The different poly- polyoses content of tremella are as follows: 94.7%；It is 18.5 ten thousand dalton through HPLC method measurement molecular weight.
Embodiment 3:
Take Tremella fructification raw material, pure water soaking and washing；15 times of volumes of raw material weight (g) are added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that concentration is 8%, alkaline hydrolysis extracts 6h under the conditions of 90 DEG C~98 DEG C；It is dense with 6% after alkaline hydrolysis
The dilute hydrochloric acid of degree adjusts material liquid PH value to 6.0~7.0；Conventional essence filtering is carried out, filtrate is collected；It is added in every 100mL filtrate
The acid flocculant solution that 10mL concentration is 0.5%, stirring makes it sufficiently flocculate under normal temperature condition, and it is compound to form a kind of precipitating
Object；Sediment composite is collected in filtering；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in raw material weight
In the pure water solution (mL) for measuring 10 times of volumes of (g), and with 5% sodium hydroxide solution adjust material liquid PH value to alkaline 8.0~
9.0, dissociation is heated under the conditions of 50 DEG C~60 DEG C.Above-mentioned feed liquid is filtered, filtrate is collected；Sediment carries out harmless treatment
Afterwards, it discards.30% hydrogen peroxide that 2.0% (V/V) of filtrate total volume is added into above-mentioned filtrate carries out decolorization；Decoloration
It is neutral that the dilute hydrochloric acid that feed liquid is again 6% with mass concentration afterwards, which adjusts pH value,；Above-mentioned feed liquid is concentrated under reduced pressure into raw material weight (g)
2 times~3 times volumes (mL)；Feed liquid is precipitated using high concentration ethanol after concentration, keeps the precipitating of ethyl alcohol in final feed liquid dense
Degree reaches 60%~70%, collects precipitating, and vacuum decompression is dry to get arriving the different poly- polysaccharide of high-purity tremella, yield are as follows:
24.8%；The different poly- polyoses content of tremella are as follows: 93.6%, it is 12.8 ten thousand dalton through HPLC method measurement molecular weight.
Embodiment 4
Take Tremella fructification raw material, pure water soaking and washing；20 times of volumes of raw material weight (g) are added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that concentration is 10%, alkaline hydrolysis extracts 10h under the conditions of 90 DEG C~98 DEG C, after alkaline hydrolysis, uses matter
The dilute hydrochloric acid for measuring concentration 6% adjusts material liquid PH value to 6.0~7.0；Conventional essence filtering is carried out, filtrate is collected；In every 100ml filtrate
The acid flocculant solution that 10ml concentration is 1% is added, stirring makes it sufficiently flocculate under normal temperature condition, and it is compound to form a kind of precipitating
Object；Sediment composite is collected in filtering；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in raw material weight
In the pure water solution (mL) for measuring 20 times of volumes of (g), and with 10% sodium hydroxide solution adjust material liquid PH value to alkaline 9.0~
10.0, dissociation is heated under the conditions of 50 DEG C~60 DEG C.Above-mentioned feed liquid is filtered, filtrate is collected；Sediment carries out harmless treatment
Afterwards, it discards.30% hydrogen peroxide that 2.0% (V/V) of feed liquid total volume is added into above-mentioned filtrate carries out decolorization；Decoloration
It is neutral that the dilute hydrochloric acid that feed liquid is again 6% with mass concentration afterwards, which adjusts pH value,；Above-mentioned feed liquid is concentrated under reduced pressure into raw material weight (g)
2 times~3 times volumes (mL)；Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, is made in final feed liquid
The concentration of volume percent of ethyl alcohol reaches 60%~70%, collects precipitating, and vacuum decompression is dry to get different poly- to high-purity tremella
Polysaccharide, yield are as follows: 26.8%；The different poly- polyoses content of tremella are as follows: 96.4%；It is 8.7 ten thousand dongles through HPLC method measurement molecular weight
.
Embodiment 5
(1) Tremella fructification raw material, pure water soaking and washing are taken；20 times of volumes of raw material weight (g) are added in raw material after cleaning
Concentration be 9% sodium hydrate aqueous solution (mL), alkaline hydrolysis extracts 9h under the conditions of 90 DEG C~98 DEG C, after alkaline hydrolysis, uses matter
The dilute hydrochloric acid for measuring concentration 6% adjusts material liquid PH value to 6.0~7.0；Conventional essence filtering is carried out, filtrate is collected；In every 100ml filtrate
The acid flocculant solution that 10ml concentration is 1% is added, stirring makes it sufficiently flocculate under normal temperature condition, and it is compound to form a kind of precipitating
Object；Sediment composite is collected in filtering；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in raw material weight
In the pure water solution (mL) for measuring 20 times of volumes of (g), and with 10% sodium hydroxide solution adjust material liquid PH value to alkaline 9.0~
10.0, dissociation is heated under the conditions of 50 DEG C~60 DEG C.Above-mentioned feed liquid is filtered, filtrate is collected；Sediment carries out harmless treatment
Afterwards, it discards.30% hydrogen peroxide that 2.0% (V/V) of feed liquid total volume is added into above-mentioned filtrate carries out decolorization；Decoloration
It is neutral that the dilute hydrochloric acid that feed liquid is again 6% with mass concentration afterwards, which adjusts pH value,；Above-mentioned feed liquid is concentrated under reduced pressure into raw material weight (g)
2 times~3 times volumes (mL)；Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, is made in final feed liquid
The concentration of volume percent of ethyl alcohol reaches 60%~70%, collects precipitating, and vacuum decompression is dry to get different poly- to high-purity tremella
Polysaccharide, yield are as follows: 24.2%；The different poly- polyoses content of tremella are as follows: 95.8%；It is 9.6 ten thousand dongles through HPLC method measurement molecular weight
.
Embodiment 6
Take Tremella fructification raw material, pure water soaking and washing；20 times of volumes of raw material weight (g) are added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that concentration is 11%, alkaline hydrolysis extracts 11h under the conditions of 90 DEG C~98 DEG C, after alkaline hydrolysis, uses matter
The dilute hydrochloric acid for measuring concentration 6% adjusts material liquid PH value to 6.0~7.0；Conventional essence filtering is carried out, filtrate is collected；In every 100ml filtrate
The acid flocculant solution that 10ml concentration is 1% is added, stirring makes it sufficiently flocculate under normal temperature condition, and it is compound to form a kind of precipitating
Object；Sediment composite is collected in filtering；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in raw material weight
In the pure water solution (mL) for measuring 20 times of volumes of (g), and with 10% sodium hydroxide solution adjust material liquid PH value to alkaline 9.0~
10.0, dissociation is heated under the conditions of 50 DEG C~60 DEG C.Above-mentioned feed liquid is filtered, filtrate is collected；Sediment carries out harmless treatment
Afterwards, it discards.30% hydrogen peroxide that 2.0% (V/V) of feed liquid total volume is added into above-mentioned filtrate carries out decolorization；Decoloration
It is neutral that the dilute hydrochloric acid that feed liquid is again 6% with mass concentration afterwards, which adjusts pH value,；Above-mentioned feed liquid is concentrated under reduced pressure into raw material weight (g)
2 times~3 times volumes (mL)；Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, is made in final feed liquid
The concentration of volume percent of ethyl alcohol reaches 60%~70%, collects precipitating, and vacuum decompression is dry to get different poly- to high-purity tremella
Polysaccharide, yield are as follows: 28.3%；The different poly- polyoses content of tremella are as follows: 94.6%；It is 5.6 ten thousand dongles through HPLC method measurement molecular weight
.
Embodiment 7:
Take Tremella fructification raw material, pure water soaking and washing；10 times of volumes of raw material weight (g) are added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that concentration is 3%, alkaline hydrolysis extracts 4h under the conditions of 90 DEG C~98 DEG C；After alkaline hydrolysis, with dilute salt
Acid adjusts material liquid PH value to 6.0~7.0；Essence filtering, collects filtrate；The acidity that 8ml concentration is 1% is added in every 100ml filtrate
Flocculant solution, stirring makes it sufficiently flocculate under normal temperature condition, forms a kind of sediment composite；Sediment composite is collected in filtering；
After filtrate progress harmless treatment, discard.The pure water that above-mentioned sediment composite is dispersed in 12 times of volumes of raw material weight (g) is molten
In liquid (mL), and material liquid PH value is adjusted to alkalinity 9.0~10.0, under the conditions of 50 DEG C~60 DEG C with 10% sodium hydroxide solution
Heating dissociation.Above-mentioned feed liquid is filtered, filtrate is collected；After sediment progress harmless treatment, discard.It is added into above-mentioned filtrate
30% hydrogen peroxide of 0.5% (V/V) of feed liquid total volume carries out decolorization；Feed liquid is with mass concentration again after decoloration
It is neutrality that 6% dilute hydrochloric acid, which adjusts pH value,；Above-mentioned feed liquid is concentrated under reduced pressure into 2 times~3 times volumes (mL) of raw material weight (g)；It is dense
Feed liquid is precipitated using 90%~95% high concentration ethanol after contracting, and the precipitating concentration of ethyl alcohol in final feed liquid is made to reach 60%
~70%, precipitating is collected, vacuum decompression is dried to arrive the different poly- polysaccharide of high-purity tremella, yield are as follows: 20.6%；Tremella is different poly-
Polyoses content are as follows: 97.1%；It is 67.5 ten thousand dalton through HPLC method measurement molecular weight.
Embodiment 8
Take Tremella fructification raw material, pure water soaking and washing；10 times of volumes of raw material weight (g) are added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that concentration is 0.5%, alkaline hydrolysis extracts 3h under the conditions of 90 DEG C~98 DEG C；After alkaline hydrolysis, use is dilute
Hydrochloric acid adjusts material liquid PH value to 6.0~7.0；Essence filtering, collects filtrate；The acid that 8ml concentration is 1% is added in every 100ml filtrate
Property flocculant solution, stirring makes it sufficiently flocculate under normal temperature condition, forms a kind of sediment composite；It is compound to collect precipitating for filtering
Object；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in the pure of 12 times of volumes of raw material weight (g)
In aqueous solution (mL), and material liquid PH value is adjusted to alkalinity 9.0~10.0, in 50 DEG C~60 DEG C items with 10% sodium hydroxide solution
Dissociation is heated under part.Above-mentioned feed liquid is filtered, filtrate is collected；After sediment progress harmless treatment, discard.Into above-mentioned filtrate
30% hydrogen peroxide that 0.5% (V/V) of feed liquid total volume is added carries out decolorization；Feed liquid uses mass concentration again after decoloration
It is neutral for 6% dilute hydrochloric acid adjusting pH value；Above-mentioned feed liquid is concentrated under reduced pressure into 2 times~3 times volumes (mL) of raw material weight (g)；
Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, reaches the precipitating concentration of ethyl alcohol in final feed liquid
60%~70%, precipitating is collected, vacuum decompression is dried to arrive the different poly- polysaccharide of high-purity tremella, yield are as follows: 22.7%；Tremella
Different poly- polyoses content are as follows: 99.2%；It is 97.1 ten thousand dalton through HPLC method measurement molecular weight.
Embodiment 9
Take Tremella fructification raw material, pure water soaking and washing；10 times of volumes of raw material weight (g) are added in raw material after cleaning
The sodium hydrate aqueous solution (mL) that concentration is 4.5%, alkaline hydrolysis extracts 6h under the conditions of 90 DEG C~98 DEG C；After alkaline hydrolysis, use is dilute
Hydrochloric acid adjusts material liquid PH value to 6.0~7.0；Essence filtering, collects filtrate；The acid that 8ml concentration is 1% is added in every 100ml filtrate
Property flocculant solution, stirring makes it sufficiently flocculate under normal temperature condition, forms a kind of sediment composite；It is compound to collect precipitating for filtering
Object；After filtrate progress harmless treatment, discard.Above-mentioned sediment composite is dispersed in the pure of 12 times of volumes of raw material weight (g)
In aqueous solution (mL), and material liquid PH value is adjusted to alkalinity 9.0~10.0, in 50 DEG C~60 DEG C items with 10% sodium hydroxide solution
Dissociation is heated under part.Above-mentioned feed liquid is filtered, filtrate is collected；After sediment progress harmless treatment, discard.Into above-mentioned filtrate
30% hydrogen peroxide that 0.5% (V/V) of feed liquid total volume is added carries out decolorization；Feed liquid uses mass concentration again after decoloration
It is neutral for 6% dilute hydrochloric acid adjusting pH value；Above-mentioned feed liquid is concentrated under reduced pressure into 2 times~3 times volumes (mL) of raw material weight (g)；
Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, reaches the precipitating concentration of ethyl alcohol in final feed liquid
60%~70%, precipitating is collected, vacuum decompression is dried to arrive the different poly- polysaccharide of high-purity tremella, yield are as follows: 25.6%；Tremella
Different poly- polyoses content are as follows: 98.5%；It is 22.4 ten thousand dalton through HPLC method measurement molecular weight.
Comparative example 1: it is prepared using the method that pure water high-temperature boiling mentions and retains the natural different poly- polysaccharide structures of tremella
The different poly- polysaccharide of high molecular weight tremella, measuring molecular weight by HPLC method is 4,800,000 dalton.Specific step is as follows:
Take Tremella fructification raw material, pure water soaking and washing；10 times of volumes of raw material weight (g) are added in raw material after cleaning
Pure water (mL) boils under the conditions of 90 DEG C~98 DEG C and mentions 8h, and filtrate is collected in filtering.Above-mentioned feed liquid is concentrated under reduced pressure into raw material weight (g)
2 times~3 times volumes (mL)；Feed liquid is precipitated using 90%~95% high concentration ethanol after concentration, is made in final feed liquid
The precipitating concentration of ethyl alcohol reaches 60%~70%, collects precipitating, and vacuum decompression is dried to arrive the different poly- polysaccharide of high-purity tremella,
Molecular weight is 4,800,000 dalton after measured.
Comparative example 2: not using acid flocculant, remaining condition and step implementation 1 according to the invention carry out, preparation
Obtain the different poly- polysaccharide of tremella.
Comparative example 3: without decolorization of the invention, remaining condition and step it is according to the invention implement 1 into
Row, is prepared the different poly- polysaccharide of tremella.
Monosaccharide composition analysis test result in the different poly- polysaccharide of 1 tremella of effect example
Test sample:
The different poly- polysaccharide of tremella made from Examples 1 to 3 and comparative example 1~2.
Test method:
(1) sample 20mg is taken, 2mol/L TFA 6ml is added, tube sealing hydrolyzes 6h at 100 DEG C, and pyridine, salt are added after being evaporated
Sugared nitrile acetic acid esters is made in sour azanol, acetic anhydride, analyzes into GC-MS, obtains mole composition of neutral monosaccharides in the different poly- polysaccharide of tremella
Ratio.
(2) mass content of the acid sugar (glucuronic acid) in each sample is tested using sulfuric acid carbazole method.
The different poly- polysaccharide of the tremella of comparative example 1 be retain the natural different poly- polysaccharide structures of tremella high molecular weight tremella it is different poly-
Polysaccharide, it can be interpreted as the tremella polysaccharides for the reset condition that do not destroyed by acid or alkali, molecule measuring is set to 4,800,000 dongles
.If the ratio and comparative example 1 by neutral sugar in the different poly- polysaccharide of low molecular weight tremella that alkaline hydrolysis obtains are almost the same, say
The monosaccharide composition and reset condition of the bright low molecule tremella polysaccharides are almost the same, i.e., different in the low molecular weight tremella obtained through alkaline hydrolysis
Substantially without introducing since cellulose hydrolyzes resulting impurity glucose in poly- polysaccharide.Specific test result: it is shown in Table 1.
Table 1: the different different poly- polysaccharide monosaccharide composition ratio analyses of tremella
| Sample | The molar ratio of neutral sugar | Glucuronic acid content, % |
| Comparative example 1 | Fucose: xylose: mannose: glucose=0.42:0.34:1:0.18 | 19 |
| Comparative example 2 | Fucose: xylose: mannose: glucose=0.25:0.26:1:0.67 | 15 |
| Embodiment 1 | Fucose: xylose: mannose: glucose=0.40:0.35:1:0.20 | 17 |
| Embodiment 2 | Fucose: xylose: mannose: glucose=0.45:0.34:1:0.17 | 20 |
| Embodiment 3 | Fucose: xylose: mannose: glucose=0.42:0.36:1:0.25 | 18 |
Found out by table 1: the neutral monosaccharides composition ratio of Examples 1 to 3 preparation gained sample is compared with sample substantially to be implemented
The sample of example 1 is consistent, and the glucose content in comparative example 2 increased significantly, and illustrates acid flocculation of the present invention
Method can effectively remove when alkaline hydrolysis extracts that (the impurity glucose may be hydrolyzed by cellulose for the impurity glucose that introduces
To).
Effect example 2: product colour comparison.
Test sample: 3 sample of comparative example and Examples 1 to 3 sample.
Test method:
Determination of light transmittance method: sample is dissolved into the aqueous solution of 0.5% mass concentration using pure water, using ultraviolet spectrometry light
Degree meter measures its light transmittance at 400nm, and saturate sample transmittance is low.As a result table 2.
The light transmittance of 2 sample of table
| Sample | Light transmittance, % |
| Comparative example 3 | 70.6 |
| Embodiment 1 | 90.7 |
| Embodiment 2 | 88.5 |
| Embodiment 3 | 92.1 |
Table 2, it is seen that implemented using the light transmittance of the different poly- polysaccharide of tremella made from the embodiment of the present invention 1~3 much higher than comparison
Example 3, therefore the different poly- saturate problem of polysaccharide of product tremella can effectively solve using preparation method of the invention.
Effect example 3: the different poly- polysaccharide of tremella is to fibroblastic activation and promotion fibroblasts to secrete hyalomitome
Acid content efficacy test
The sample of above-described embodiment 1 is selected to carry out efficacy test.
It with the DMEM culture medium culture human fibroblasts containing calf serum, passes on 2 times, confirmation cell growth status is steady
It is fixed, take the cell that growth fusion rate is 80% or so stand-by.
The cell for growing to 80% or so in culture dish is recycled in centrifuge tube in aseptic operating platform, passes through cytometer
Number instrument meter number, is seeded in 6 porocyte culture plates and is cultivated, and adds the extract of embodiment 1 in the medium, is training it
The concentration (w/v) supported in base is 0.1% (i.e. 0.1g/100mL) and 0.01%, while setting up and not adding extract of the invention
One group is blank control group.
After culture 3 days, every hole cell quantity is measured with cell counter, and for statistical analysis.
Hyaluronic acid contents detection: the culture solution after collecting each component 72h carries out the ELISA detection of hyaluronic acid (HA)
(i.e. enzyme-linked immunosorbent assay detection) draws standard items and draws standard song, and by specification is added reagent, is protected from light incubation at room temperature
The reacting terminating solution of 5min terminates the concentration that observation absorptance in reaction 15min measures HA.
Testing result:
(1) fibroblast proliferation effect: sample after the culture of 72h, containing sample concentration 0.5%, 0.25%,
The cell survival rate of 0.125% effect group is 107.03%, 106.31%, 105.70%, shows to have fibroblast
Good cultivation effect.
(2) fibroblasts to secrete hyaluronic acid effect: sample after the culture of 72h, containing sample concentration 0.5%,
0.25%, the hyaluronic acid secretion amount of 0.125% effect group is 108.57%, 106.83%, 100.76%, therefore, 0.5%,
The different poly- polysaccharide of the tremella of 0.25% concentration can significantly promote fibroblasts to secrete hyaluronic acid.
The different poly- polysaccharide of the tremella that embodiment 1 is prepared it can be seen from the above testing result can promote fibroblast
Proliferation, while can increase skin skin corium storage capacity by promoting fibroblasts to secrete hyaluronic acid to reach, reach
Deep water supplement, the effect of anti-aging.
Effect example 4: the different poly- polysaccharide percutaneous permeability comparison of tremella
1, test equipment and reagent:
Permeameter (RYJ-6B type drug transdermal diffusion test instrument)
Artificial skin (Shanghai pavilion instrument instrument)
2, test sample: comparative example 1 and Examples 1 to 3 sample
3, test method
Percutaneous dispersion test instrument acceptance pool volume is 15mL, and acceptable solution is the PBS buffer solution of PH 6.0, and diffusion cell is placed in
37 DEG C are kept in water bath with thermostatic control circulating box, are stirred continuously with 400r/min.Acceptable solution is added in acceptance pool and and artificial skin
Close contact, and pay attention to draining bubble from sample tap.After starting diffusion tester 30min stabilization, 3ml sample solution is added
Enter sample cell, be stirred continuously with 400r/min, continuously permeate 8h, infiltration terminates, and reception tank solution is taken to survey using Phenol sulfuric acid procedure
Determine the content of tremella polysaccharides.Using the accumulation transmitance of 8h as the infiltrative evaluation condition of sample.
8h accumulates transmitance and calculates: the amount (mg) of reception tank tremella polysaccharides/sample cell initial tremella polysaccharides amount (mg)
Wherein, determination of polysaccharide refers to: " saccharide complex biochemical investigations technique ", publishing house, Zhang Weijie Zhejiang University,
1999.
4, test result is shown in Table 3:
The different poly- polysaccharide transdermal rate of 3 tremella of table
| Sample | 8h accumulates transmitance, % |
| Comparative example 1 | 5.9 |
| Embodiment 1 | 36.7 |
| Embodiment 2 | 32.4 |
| Embodiment 3 | 38.1 |
Table 3 can be shown that the different poly- polysaccharide of tremella that 1~3 middle-molecular-weihydroxyethyl of the embodiment of the present application is 10~200,000 shows good
Good percutaneous permeability, the different poly- polysaccharide of high molecular weight tremella being much better than in comparative example 1.Good percutaneous permeability can make
The different poly- polysaccharide of 10~200,000 tremella in the application preferably penetrates skin, and by promoting fibroblast proliferation, promoting
Fibroblasts to secrete hyaluronic acid achievees the effect that deep water supplement, anti-aging to increase skin skin corium storage capacity.
Claims (10)
1. a kind of preparation method of the different poly- polyoses extract of tremella, which comprises the following steps: by acid flocculant with
The tremella alkali extract mixing of faintly acid or neutrality is adjusted to through acid, flocculation is separated by solid-liquid separation to obtain sediment composite, the precipitating is answered
It closes object to dissociate under alkaline condition, is separated by solid-liquid separation, obtains filtrate A；Wherein, alkali used in the tremella alkali extract is quality
The sodium hydrate aqueous solution that concentration is 5%~8%, the acid flocculant are the aqueous acid of flocculant, the flocculation
Agent is chitosan, the specification of the chitosan are as follows: deacetylation >=85%；Viscosity and at 20 DEG C, when concentration is 10 grams per liter
>=200mpa.s, after the dissociation is the following steps are included: the sediment composite is mixed with water, being adjusted to pH with alkali is
It is dissociated at 8.0~10.0,50~60 DEG C.
2. the preparation method of the different poly- polyoses extract of tremella as described in claim 1, which is characterized in that
In the acid flocculant, the flocculant concentration is 0.005~0.02g/mL；
And/or in the acid flocculant, the sour water is aqueous hydrochloric acid solution, and the quality of the aqueous hydrochloric acid solution is dense
Degree is 0.25%~1.0%；
And/or described " the tremella alkali extract of faintly acid or neutrality is adjusted to through acid " the preparation method comprises the following steps: tremella is carried out alkaline hydrolysis
After obtaining tremella alkali extract, it is adjusted with acid the tremella alkali extract to faintly acid or neutrality, the filtrate being obtained by filtration, as institute
" the tremella alkali extract of faintly acid or neutrality is adjusted to through acid " stated；
And/or the acid in described " being adjusted to the tremella alkali extract of faintly acid or neutrality through acid " is dilute hydrochloric acid；The dilute hydrochloric acid
Mass concentration be 0.5%~6.0%；
And/or mixing of described " the tremella alkali extract of faintly acid or neutrality is adjusted to through acid " with " the acid flocculant "
Mode is to be added to the acid flocculant in described " being adjusted to the tremella alkali extract of faintly acid or neutrality through acid "；
And/or the dosage of the acid flocculant are as follows: " the tremella alkali of faintly acid or neutrality is adjusted to through acid described in every 100mL
Acid flocculant described in 5~10mL of addition in extract "；
And/or the flocculated temperature is 10~35 DEG C；
And/or the tremella is Tremella fructification raw material.
3. the preparation method of the different poly- polyoses extract of tremella as described in claim 1, which is characterized in that described " adjusts through acid
To faintly acid or neutrality tremella alkali extract " in acid be dilute hydrochloric acid；The mass concentration of the dilute hydrochloric acid is 1%~6.0%；
And/or the dosage of the acid flocculant are as follows: " the tremella alkali of faintly acid or neutrality is adjusted to through acid described in every 100mL
Acid flocculant described in 5~8mL of addition in extract ".
4. the preparation method of the different poly- polyoses extract of tremella as described in claim 1, which is characterized in that
In the preparation of the filtrate A, the volume mass ratio of the water and the sediment composite is 10~20mL/g；
And/or in the preparation of the filtrate A, the alkali is sodium hydroxide；The sodium hydroxide is that mass concentration is
4%~10% sodium hydrate aqueous solution；
And/or in the preparation of the filtrate A, described " being adjusted to PH is 8.0~10.0 " is 8.0~9.0 to be adjusted to PH.
5. the preparation method of the different poly- polyoses extract of tremella as described in claim 1, which is characterized in that the filtrate A's
In preparation, the volume mass ratio of the water and the sediment composite is 12~15mL/g.
6. a kind of preparation method of the different poly- polysaccharide of tremella comprising following steps: by the described in any item filters of Claims 1 to 5
Liquid A decoloration, is adjusted to neutrality with acid, is concentrated, alcohol precipitation, collects precipitating.
7. the preparation method of the different poly- polysaccharide of tremella as claimed in claim 6, which is characterized in that the decoloration is using peroxide
Change hydrogen to decolourize；The hydrogen peroxide is the aqueous hydrogen peroxide solution that mass fraction is 30%；The mass fraction is
The volume of 30% hydrogen peroxide is the 0.5%~2.0% of the filtrate A volume；
And/or in the preparation method of the different poly- polysaccharide of tremella, the acid is dilute hydrochloric acid；The quality of the dilute hydrochloric acid
Concentration is 4%~8%；
And/or in the preparation method of the different poly- polysaccharide of tremella, the concentration is to be concentrated under reduced pressure；The concentration is extremely
The volume mass of concentrate and tremella ratio is 2~3mL/g, and the tremella is used in the preparation process of the filtrate A
Tremella raw material；
And/or the alcohol is precipitated as being precipitated using ethyl alcohol, the ethyl alcohol be concentration of volume percent be 90%~
95% ethanol water；The concentration of volume percent of the terminal of the alcohol precipitation to final ethyl alcohol reaches 60%~70%；
And/or in the preparation method of the different poly- polysaccharide of tremella, further include the steps that the dry precipitating；Described is dry
Dry vacuum decompression is dry.
8. the preparation method of the different poly- polysaccharide of tremella as claimed in claims 6 or 7, which is characterized in that the preparation of the filtrate A
The alkali carries condition of tremella alkali extract is prepared in the process are as follows: alkali is the sodium hydrate aqueous solution that mass concentration is 5%~8%；It is described
Buck and the volume mass ratio of the tremella be 15~25mL/g；The temperature of alkaline hydrolysis is 90~98 DEG C；The time of alkaline hydrolysis is 3
~6h；Molecular weight 10~different poly- polysaccharide of 200,000 dalton tremellas is prepared.
9. a kind of molecular weight that the preparation method of the different poly- polysaccharide of tremella as claimed in claim 8 is prepared is 10~200,000
The different poly- polysaccharide of tremella that you pause.
10. a kind of molecular weight as claimed in claim 9 is the different poly- polysaccharide of tremella of 10~200,000 dalton in skin care item
It include deep water supplement and/or anti-aging using the effect of, described skin care item.
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| CN101780118A (en) * | 2009-01-19 | 2010-07-21 | 华东理工大学 | Method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation |
| CN104211829A (en) * | 2014-09-17 | 2014-12-17 | 上海交通大学 | Preparation and application of white fungus functional polysaccharide |
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| CN101780118A (en) * | 2009-01-19 | 2010-07-21 | 华东理工大学 | Method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation |
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