CN107007498A - A kind of anti crease and anti senile tea oil extract and preparation method thereof - Google Patents
A kind of anti crease and anti senile tea oil extract and preparation method thereof Download PDFInfo
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- CN107007498A CN107007498A CN201710386452.1A CN201710386452A CN107007498A CN 107007498 A CN107007498 A CN 107007498A CN 201710386452 A CN201710386452 A CN 201710386452A CN 107007498 A CN107007498 A CN 107007498A
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- tea oil
- crease
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- 239000010495 camellia oil Substances 0.000 title claims abstract description 116
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 238000000605 extraction Methods 0.000 title claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000002904 solvent Substances 0.000 claims abstract description 23
- 238000007127 saponification reaction Methods 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000000706 filtrate Substances 0.000 claims abstract description 10
- 238000005374 membrane filtration Methods 0.000 claims abstract description 10
- 238000001694 spray drying Methods 0.000 claims abstract description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical group CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 27
- 239000000203 mixture Substances 0.000 claims description 10
- 238000001471 micro-filtration Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 2
- 230000003005 anti-senility effect Effects 0.000 claims 1
- 230000037303 wrinkles Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
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- 239000004519 grease Substances 0.000 description 2
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- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 235000020688 green tea extract Nutrition 0.000 description 1
- 229940094952 green tea extract Drugs 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
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- 208000014674 injury Diseases 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 229940029329 intrinsic factor Drugs 0.000 description 1
- 229940041476 lactose 100 mg Drugs 0.000 description 1
- 150000002617 leukotrienes Chemical class 0.000 description 1
- 238000010630 lipid peroxidation (MDA) assay Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
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- 229940118019 malondialdehyde Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Birds (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to technical field of biological extraction, more particularly to a kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：1) it is raw material to take camellia oil, adds the ethanol solution of alkalescence, heats saponification, is cooled to room temperature；2) solvent of 9~16 times of volumes of camellia oil, HIGH PRESSURE TREATMENT are added；3) ultrasonic wave extraction；4) micro-filtrate membrane filtration is carried out；5) it is concentrated under reduced pressure, spray drying.The present invention also provides the anti crease and anti senile tea oil extract for being prepared by the method for the present invention and obtaining.Obtained tea oil extract, which is prepared by the method for the present invention, has significant anti crease and anti senile old effect, and shelf-stable, and application is big.
Description
Technical field
The invention belongs to technical field of biological extraction, more particularly to a kind of anti crease and anti senile tea oil extract and its preparation side
Method.
Background technology
Camellia oil (also known as Wild camellia seed oil, tea-seed oil, camellia seed oil) is derived from the seed of tea oil tree.The manufacturing process of camellia oil
It can be divided into：Shell, dry, crush, extract oil, filtering, overall process is physical method, therefore it is real fully natural green food
With oil.Erucic acid, cholesterol, aflatoxins and other additives are free of in tea oil.After tested：Unrighted acid is up in tea oil
More than 90%, oleic acid reaches 80~83%, and linoleic acid reaches 7~13%, and is rich in protein and vitamin A, B, D, E etc., especially
It is that abundant leukotrienes contained by it is needed by human and can not synthesized.
Tea oil has five big characteristics,
1. the edible oil and fat with pollution-free food property：Tea oil is also known as camellia seed oil, and short, bristly hair or beard (cha) oil originates from wild tree
Kind-oil tea (tree), the tree is grown in high and steep mountains, it is not necessary to which labor management (as applied fertilizer, is sprayed insecticide), without any pollution.
2. it is easy to human consumption's absorption：In conventional edible oil and fat, high-quality aliphatic acid contained by tea oil at most (reaches 90.4
℅), saturated fatty acid (being commonly called as fat) content is minimum, without heavy erucic acid, is understood according to grease theory, tea oil portion big absolutely
Split-off, which is supported, to be easy to tissue and quickly absorbs.This property is also referred to as " skin-friendly ".
3. significant reducing blood lipid performance：Practical basis：No less than the practical experience of life of tens of millions of people.Because tea oil can be removed
Harmful blood fat (saturated fatty acid and cholesterol), so the effects such as it has blood clean, reduction blood viscosity and dredging vascellum.
The inoxidizability of the last 4.：Tea oil be typical oleic acid/linoleic acid grease, stronger inoxidizability be it is such not
The general character of saturated fatty acid；The vitamin E enriched contained by tea oil is generally acknowledged powerful antioxidant.
5. good biomembrane fluidity：Because tea oil has significant reducing blood lipid performance, so it can remove attachment
The metabolism of lipid and cholesterol on wall, dredges capillary and arteries in the blood vessels, and blood is moistened in increase blood vessel blood transfusion section, softening
Pipe, recovers its elasticity, blood vessel is kept young state.
And general camellia oil is divided into edible camellia oil and external-use skin care tea oil, edible-type tea oil typically selects fresh tea oil, always
Tea oil is used for skin effect more preferably, new tea oil alkalescence, and edible alkalescence is to healthy.By prolonged storage, tea oil
Acid value increase, become weakly acidic.The skin of people inherently weak acid, the cosmetics of weak acid are good to skin, so older
Tea oil to skin more it is good it is easier absorb.The general camellia oil seal that squeeze and filter is good, which is placed more than 10 years, to be referred to as always
Tea oil.
At present, existing many factors are proved relevant with aging, can be divided into Castle's intrinsic factor and extrinsic factor, wherein endogenous
Sexual factor maximum is injury of the free radical to body, for skin, and the maximum factor of exogenous aging is ultraviolet, outside
Property aging in source is otherwise known as " light aging ".When talking about anti-aging, we usually can be since anti-oxidant, removing free radical, freely
Base be a class very active bioactive molecule group (because the overwhelming majority contains free oxygen atom, be generally called oxygen from
By base) be typically body metabolism product.Free radical is endogenous aging factor be determined at present, most important, therefore, is resisted
Aging is exactly Green Tea Extract, anti-oxidant largely.If for external-use skin care, camellia oil needs sealing more than 10 years,
Need the time long, just can be more preferable for external-use skin care effect, even so, the camellia oil of oily is used for direct skin care, oil
Soapy feeling is not to be easy to allow people to receive.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of anti crease and anti senile tea oil extract and preparation method thereof, pass through
This method prepares anti crease and anti senile tea oil extract, and obtained product can be applied directly in cosmetics or skin care item, it is not necessary to passed through
Sealed after for many years, and the shelf-life significantly extends, and its application will not be limited due to the greasy feeling of tea oil.
To achieve the above object, the present invention is adopted the following technical scheme that：
The present invention provides a kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, adds the ethanol solution of alkalescence, heats saponification, is cooled to room temperature；
2) solvent of 9~16 times of volumes of camellia oil, HIGH PRESSURE TREATMENT are added；
3) ultrasonic wave extraction；
4) micro-filtrate membrane filtration is carried out；
5) it is concentrated under reduced pressure, spray drying.
Preferably, step 1 of the present invention) in, the solute in alkaline ethanol solution is sodium hydroxide.Alkaline ethanol is molten
Liquid is added, and is more beneficial for saponification.
Preferably, step 1 of the present invention) in, the concentration of alkaline ethanol solution is 0.7~1.2mol/L.Concentration thing
Preferably, the progress of reaction is conducive to.
Preferably, step 1 of the present invention) in, the volume ratio of the ethanol solution of camellia oil and alkalescence is 1:15~25.It is logical
Such volume ratio is crossed, can more accelerate reaction speed.
Preferably, step 1 of the present invention) in, it is heated to 75~95 DEG C of 40~65min of saponification.At such temperature, instead
Should be fastest, effect is best.
Preferably, step 2 of the present invention) in, the ethanol that solvent is ethyl acetate and volume fraction is 65~75% is molten
The mixture of liquid.Selection preferably solvent is arranged in pairs or groups, and preferably, recovery rate is also maximum for the effect of effective component extracting.
It is further preferable that it is 1 that the solvent, which is volume ratio,:3~6 ethyl acetate and the mixture of ethanol solution.Selection
Preferably solvent burden ratio, preferably, recovery rate is also maximum for the effect of effective component extracting.
Preferably, step 2 of the present invention) in, 2~6min of HIGH PRESSURE TREATMENT under 78~90MPa.Under HIGH PRESSURE TREATMENT,
Under high pressure effect, cell rupture, active ingredient is separated out, and is more beneficial for improving effective component extraction rate, while being also follow-up
Extraction is ready, and shortens manufacturing cycle.
Preferably, step 3 of the present invention) in, 10~18min of ultrasonic wave extraction under 230~420W.At high pressure
After reason, ultrasonic wave extraction is selected, being capable of effective component extracting, the extract prepared, anti crease and anti senile old effect to greatest extent
It is preferably also.
Preferably, step 4 of the present invention) in, the molecular weight that blocks of microfiltration membranes is 100000~250000D, and flow velocity is 2
~4ml/min.By micro-filtration, impurity screening can further refine extract.
The present invention also provides the anti crease and anti senile tea oil that the preparation method of described anti crease and anti senile tea oil extract is prepared
Extract.Anti crease and anti senile tea oil extract is prepared by the method for the present invention, it is not easy to become sour, greatly prolong usage cycles, meanwhile,
There is no greasy feeling, can be used in various anti crease and anti senile old or improve in anti crease and anti senile old product, expand application.
Anti crease and anti senile old tea oil extract is prepared by the method for the present invention, sealed storage for many years is being needed not move through, so that it may
To be directly used in skin care item, and effect is significantly, furthermore it is possible to significantly improve the useful life of tea oil, it is not easy to become sour, also may be used
It is used for as raw material in food, skin care item or cosmetics, expands application.Obtained in addition, being prepared by the method for the present invention
Tea oil extract there is preferable anti crease and anti senile old effect.
Embodiment
The invention will be further described with reference to embodiments, but the invention is not limited in these embodiments.
Embodiment 1
A kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, and the concentration for adding 15 times of camellia oil volume is 1.2mol/L, and solute is sodium hydroxide
The ethanol solution of alkalescence, is heated to 75 DEG C of saponification 65min, is cooled to room temperature；
2) solvent of 9 times of volumes of camellia oil is added, the HIGH PRESSURE TREATMENT 2min under 90MPa, the solvent is that volume ratio is 1:5
Ethyl acetate and volume fraction for 65% ethanol solution mixture；
3) the ultrasonic wave extraction 10min under 420W；
4) with the micro-filtrate membrane filtration that molecular weight is 250000D is blocked, flow velocity is 2ml/min；
5) it is concentrated under reduced pressure, spray drying is available anti crease and anti senile tea oil extract.
Embodiment 2
A kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, and the concentration for adding 18 times of camellia oil volume is 1.1mol/L, and solute is sodium hydroxide
The ethanol solution of alkalescence, is heated to 80 DEG C of saponification 55min, is cooled to room temperature；
2) solvent of 11 times of volumes of camellia oil is added, the HIGH PRESSURE TREATMENT 3min under 87MPa, the solvent is that volume ratio is 1:
6 ethyl acetate and volume fraction is the mixture of 70% ethanol solution；
3) the ultrasonic wave extraction 12min under 370W；
4) with the micro-filtrate membrane filtration that molecular weight is 220000D is blocked, flow velocity is 3ml/min；
5) it is concentrated under reduced pressure, spray drying is available anti crease and anti senile tea oil extract.
Embodiment 3
A kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, and the concentration for adding 20 times of camellia oil volume is 0.9mol/L, and solute is sodium hydroxide
The ethanol solution of alkalescence, is heated to 85 DEG C of saponification 50min, is cooled to room temperature；
2) solvent of 13 times of volumes of camellia oil is added, the HIGH PRESSURE TREATMENT 4min under 84MPa, the solvent is that volume ratio is 1:
5 ethyl acetate and volume fraction is the mixture of 70% ethanol solution；
3) the ultrasonic wave extraction 14min under 320W；
4) with the micro-filtrate membrane filtration that molecular weight is 180000D is blocked, flow velocity is 3ml/min；
5) it is concentrated under reduced pressure, spray drying is available anti crease and anti senile tea oil extract.
Embodiment 4
A kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, and the concentration for adding 23 times of camellia oil volume is 0.8mol/L, and solute is sodium hydroxide
The ethanol solution of alkalescence, is heated to 90 DEG C of saponification 45min, is cooled to room temperature；
2) solvent of 15 times of volumes of camellia oil is added, the HIGH PRESSURE TREATMENT 5min under 81MPa, the solvent is that volume ratio is 1:
4 ethyl acetate and volume fraction is the mixture of 75% ethanol solution；
3) the ultrasonic wave extraction 16min under 280W；
4) with the micro-filtrate membrane filtration that molecular weight is 140000D is blocked, flow velocity is 4ml/min；
5) it is concentrated under reduced pressure, spray drying is available anti crease and anti senile tea oil extract.
Embodiment 5
A kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, and the concentration for adding 25 times of camellia oil volume is 0.7mol/L, and solute is sodium hydroxide
The ethanol solution of alkalescence, is heated to 95 DEG C of saponification 40min, is cooled to room temperature；
2) solvent of 16 times of volumes of camellia oil is added, the HIGH PRESSURE TREATMENT 6min under 78MPa, the solvent is that volume ratio is 1:
3 ethyl acetate and volume fraction is the mixture of 65% ethanol solution；
3) the ultrasonic wave extraction 18min under 230W；
4) with the micro-filtrate membrane filtration that molecular weight is 100000D is blocked, flow velocity is 2ml/min；
5) it is concentrated under reduced pressure, spray drying is available anti crease and anti senile tea oil extract.
Comparative example 1
A kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, and the concentration for adding 20 times of camellia oil volume is 0.9mol/L, and solute is sodium hydroxide
The ethanol solution of alkalescence, is heated to 85 DEG C of saponification 50min, is cooled to room temperature；
2) solvent of 13 times of volumes of camellia oil is added, the ultrasonic wave extraction 14min under 320W, the solvent is that volume ratio is
1:5 ethyl acetate and volume fraction is the mixture of 70% ethanol solution；
3) with the micro-filtrate membrane filtration that molecular weight is 180000D is blocked, flow velocity is 3ml/min；
4) it is concentrated under reduced pressure, spray drying is available anti crease and anti senile tea oil extract.
Comparative example 2
A kind of preparation method of anti crease and anti senile tea oil extract, including following preparation process：
1) it is raw material to take camellia oil, and the concentration for adding 20 times of camellia oil volume is 0.9mol/L, and solute is sodium hydroxide
The ethanol solution of alkalescence, is heated to 85 DEG C of saponification 50min, is cooled to room temperature；
2) solvent of 13 times of volumes of camellia oil is added, the HIGH PRESSURE TREATMENT 4min under 84MPa, the solvent is that volume ratio is 1:
5 ethyl acetate and volume fraction is the mixture of 70% ethanol solution；
3) refluxing extraction 3 times at 85 DEG C, every time 1 hour, merge extract solution；
4) with the micro-filtrate membrane filtration that molecular weight is 180000D is blocked, flow velocity is 3ml/min；
5) it is concentrated under reduced pressure, spray drying is available anti crease and anti senile tea oil extract.
Test example 1：The external anti-aging experiment of anti crease and anti senile tea oil extract that the inventive method is prepared
1st, modeling and administration
Kunming mouse 40 is taken, body weight 22-26g is provided by Guangxi Medical University's experimental animal center, is randomly divided into 5
Group (normal group, model group, 3 groups of embodiment, 1 group of comparative example, 2 groups of comparative example), every group 8.Normal group, the isometric physiology of gavage
Salt solution；D- galactolipins 100mg/kg and the isometric physiological saline of gavage is subcutaneously injected in model group, Kunming mouse nape part；Implement
The tea that D- galactolipins 100mg/kg and the isometric embodiment 3 of gavage are prepared is subcutaneously injected in 3 groups of example, Kunming mouse nape part
Oil extract 100mg/kg；The body such as D- galactolipins 100mg/kg and gavage is subcutaneously injected in 1 group of comparative example, Kunming mouse nape part
The tea oil extract 100mg/kg that product comparative example 1 is prepared；D- half is subcutaneously injected in 2 groups of comparative example, Kunming mouse nape part
The tea oil extract 100mg/kg that lactose 100mg/kg and the isometric comparative example 2 of gavage are prepared；By daily 20mL/kg agent
Amount, one day gavage once, successive administration 50d.(embodiment 3, comparative example 1 and comparative example 2 concentration as needed for being prepared solvent using pure water
Sample)
2nd, mice serum SOD vigor and MDA assays
After 50d last doses 2h, blood is taken in Kunming mouse eyeball rear vein beard capillary, centrifuges take blood immediately
Clearly, operated by superoxide dismutase kit specification, each pipe absorbance is determined at 550nm, calculate super oxygen in serum
Thing is disproportionated enzyme activity.Operated by Malondialdehyde Kit specification, the third two are determined in each pipe absorbance, calculating serum 532nm at
Aldehyde.
3rd, murine liver tissue GSH-Px vitality tests
Mouse liver is taken, connective tissue is removed, the tissue that the physiological saline of homogenizer grinding plus precooling is prepared into 10% is even
Slurry.Refrigerated centrifuge (2 DEG C~4 DEG C), takes its supernatant, with glutathione peroxidase kit measurement glutathione peroxidating
Thing enzyme activity.
4th, Mice brain tissues CAT vitality tests
Kunming mouse takes rapid cervical dislocation after blood to put to death, and cuts brain tissue, with using filter paper after 4 DEG C of normal saline flushings
Blot, -20 DEG C of freezen protectives.During measure, brain tissue homogenate, 3000r/min refrigerated centrifuge 10min at 4 DEG C take its supernatant,
Activity of catalase is determined with hydrogen peroxide enzyme reagent kit.
5th, result of the test
Each group rat blood serum SOD, MDA, GSH-Px and brain tissue LPF changes of contents
Experiment above is understood, anti crease and anti senile tea oil extract is prepared using the inventive method, and it has preferably anti-ageing
Old effect.
Test example 2：The anti crease and anti senile old tea oil extract that the inventive method is prepared irradiates lower cavy skin to ultraviolet
The protective action of skin
30 cavys are randomly divided into 5 groups：
A groups are negative control group, the anti crease and anti senile old tea oil extract of the tested area's not external application of guinea pig back and do not irradiate ultraviolet
Line；
B groups are irradiation control group, the anti crease and anti senile old tea oil extract of the tested area's not external application of guinea pig back, but receive ultraviolet
Irradiation；
C groups are the anti crease and anti senile old tea oil extract group of external application embodiment 3, and the tested area's external application embodiment 3 of guinea pig back is crease-resistant
Anti-aging tea oil extract is followed by being irradiated by ultraviolet.
D is the anti crease and anti senile old tea oil extract group of external application comparative example 1, and the tested area's external application comparative example 1 of guinea pig back is crease-resistant anti-
Aging tea oil extract is followed by being irradiated by ultraviolet.
E groups are the anti crease and anti senile old tea oil extract group of external application comparative example 2, and the tested area's external application comparative example 2 of guinea pig back is crease-resistant
Anti-aging tea oil extract is followed by being irradiated by ultraviolet.
In the anti crease and anti senile old tea oil extract of the external application present invention, add pure water and stir into after pasty state, then applied
Smear.
Each pre-irradiation, light is shaved by guinea pig back skin 5cm × 6cm scope internal hairs, and C groups apply embodiment 3 with swab stick and are made
Anti crease and anti senile old tea oil extract, D groups with swab stick apply comparative example 1 made from anti crease and anti senile old tea oil extract, E group swab sticks
Anti crease and anti senile old tea oil extract made from comparative example 2 is applied, control group applies water.Tested cavy is put into irradiation case, irradiated simultaneously
Long wave ultraviolet (UVA) and ultraviolet B radiation (UVB), irradiate 3 times weekly, concurrent irradiation 15 weeks.
A group guinea pig skins do not occur exception.
After B groups are irradiated 2 weeks through ultraviolet, irradiated site starts erythema occur, with the extension of irradiation time, erythema area
Gradually expand and aggravate.
Just there is erythema after irradiating 5 weeks in C groups, and erythema area is significantly less than B groups.
There is erythema after irradiating 4 weeks in D groups, but erythema area is less than B groups.
Just there is erythema after irradiating 4 weeks in E groups, and erythema area is significantly less than B groups.
This test example confirms that the anti crease and anti senile old tea oil extract that the inventive method is prepared passes through external preparation for skin approach
Available for protecting the skin from the oxidative damages such as ultraviolet radiation, and thus caused skin ageing phenomenon is resisted, simultaneously explanation
The anti crease and anti senile old tea oil extract prepared by the method for the present invention is needed not move through to be sealed for many years, is just had good
Effect.
Claims (10)
1. a kind of preparation method of anti crease and anti senile tea oil extract, it is characterised in that including following preparation process：
1) it is raw material to take camellia oil, adds the ethanol solution of alkalescence, heats saponification, is cooled to room temperature；
2) solvent of 9~16 times of volumes of camellia oil, HIGH PRESSURE TREATMENT are added；
3) ultrasonic wave extraction；
4) micro-filtrate membrane filtration is carried out；
5) it is concentrated under reduced pressure, spray drying.
2. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 1)
In, the solute in alkaline ethanol solution is sodium hydroxide.
3. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 1)
In, the concentration of alkaline ethanol solution is 0.7~1.2mol/L.
4. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 1)
In, the volume ratio of the ethanol solution of camellia oil and alkalescence is 1:15~25.
5. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 1)
In, it is heated to 75~95 DEG C of 40~65min of saponification.
6. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 2)
In, the mixture for the ethanol solution that solvent is ethyl acetate and volume fraction is 65~75%.
7. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 2)
In, 2~6min of HIGH PRESSURE TREATMENT under 78~90MPa.
8. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 3)
In, 10~18min of ultrasonic wave extraction under 230~420W.
9. the preparation method of anti crease and anti senile tea oil extract according to claim 1, it is characterised in that：The step 4)
In, the molecular weight that blocks of microfiltration membranes is 100000~250000D, and flow velocity is 2~4ml/min.
It is anti-that 10. the preparation method of the anti crease and anti senile tea oil extract according to claim 1~9 any one is prepared
Wrinkle antisenility tea oil extract.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305974A (en) * | 2008-07-11 | 2008-11-19 | 广东新大地生物科技有限公司 | Camellia oil preparation method for cosmetics |
| CN104546609A (en) * | 2015-01-09 | 2015-04-29 | 湖南新金浩茶油股份有限公司 | Anti-wrinkle and nursing camellia oleifera oil |
| CN104855539A (en) * | 2015-05-07 | 2015-08-26 | 含山县昭关鹰皇油脂有限公司 | Anti-wrinkle beauty maintaining blend oil |
-
2017
- 2017-05-26 CN CN201710386452.1A patent/CN107007498A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305974A (en) * | 2008-07-11 | 2008-11-19 | 广东新大地生物科技有限公司 | Camellia oil preparation method for cosmetics |
| CN104546609A (en) * | 2015-01-09 | 2015-04-29 | 湖南新金浩茶油股份有限公司 | Anti-wrinkle and nursing camellia oleifera oil |
| CN104855539A (en) * | 2015-05-07 | 2015-08-26 | 含山县昭关鹰皇油脂有限公司 | Anti-wrinkle beauty maintaining blend oil |
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