CN107002120B - 测序方法 - Google Patents

测序方法 Download PDF

Info

Publication number
CN107002120B
CN107002120B CN201580039895.2A CN201580039895A CN107002120B CN 107002120 B CN107002120 B CN 107002120B CN 201580039895 A CN201580039895 A CN 201580039895A CN 107002120 B CN107002120 B CN 107002120B
Authority
CN
China
Prior art keywords
nucleic acid
template nucleic
sequence
acid molecules
molecular marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580039895.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN107002120A (zh
Inventor
凯瑟琳·默里·伯克
艾伦·厄尔·达尔林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Singapore Pte Ltd
Original Assignee
Langs Technology Co ltd
University of Technology Sydney
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51177418&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN107002120(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Langs Technology Co ltd, University of Technology Sydney filed Critical Langs Technology Co ltd
Priority to CN202210864432.1A priority Critical patent/CN116072218A/zh
Publication of CN107002120A publication Critical patent/CN107002120A/zh
Application granted granted Critical
Publication of CN107002120B publication Critical patent/CN107002120B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioethics (AREA)
  • Databases & Information Systems (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580039895.2A 2014-05-23 2015-05-22 测序方法 Active CN107002120B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210864432.1A CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1409282.9A GB201409282D0 (en) 2014-05-23 2014-05-23 Sequencing process
GB1409282.9 2014-05-23
PCT/GB2015/051518 WO2015177570A1 (en) 2014-05-23 2015-05-22 Sequencing process

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202210864432.1A Division CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

Publications (2)

Publication Number Publication Date
CN107002120A CN107002120A (zh) 2017-08-01
CN107002120B true CN107002120B (zh) 2022-08-09

Family

ID=51177418

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201580039895.2A Active CN107002120B (zh) 2014-05-23 2015-05-22 测序方法
CN202210864432.1A Pending CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202210864432.1A Pending CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

Country Status (12)

Country Link
US (2) US11047002B2 (https=)
EP (1) EP3146070B2 (https=)
JP (1) JP6687605B2 (https=)
KR (3) KR102363284B1 (https=)
CN (2) CN107002120B (https=)
AU (1) AU2015263055B2 (https=)
CA (1) CA2949925A1 (https=)
DK (1) DK3146070T5 (https=)
ES (1) ES2697804T5 (https=)
GB (1) GB201409282D0 (https=)
SG (1) SG11201609754QA (https=)
WO (1) WO2015177570A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2979395T3 (es) 2016-05-26 2024-09-25 Becton Dickinson Co Métodos de ajuste del recuento de etiquetas moleculares
JP7228510B2 (ja) * 2016-11-08 2023-02-24 ベクトン・ディキンソン・アンド・カンパニー 細胞標識分類の方法
US11164659B2 (en) 2016-11-08 2021-11-02 Becton, Dickinson And Company Methods for expression profile classification
KR101798229B1 (ko) * 2016-12-27 2017-12-12 주식회사 천랩 전장 리보솜 rna 서열정보를 얻는 방법 및 상기 리보솜 rna 서열정보를 이용하여 미생물을 동정하는 방법
CN111108218B (zh) * 2017-09-20 2024-06-25 生命科技股份有限公司 使用压缩的分子标记的核酸序列数据检测融合的方法
CN111247589B (zh) * 2017-09-25 2025-06-03 贝克顿迪金森公司 免疫受体条形码错误校正
KR102878365B1 (ko) * 2017-10-09 2025-10-31 주식회사 마크로젠 단일 분자 서열분석 및 핵산서열 특성화를 위한 고유한 분자 식별자
JPWO2019132010A1 (ja) * 2017-12-28 2021-01-21 タカラバイオ株式会社 塩基配列における塩基種を推定する方法、装置及びプログラム
US12221657B2 (en) 2018-08-10 2025-02-11 Tata Consultancy Services Limited Method and system for improving amplicon sequencing based taxonomic resolution of microbial communities
ES2965194T3 (es) * 2018-08-13 2024-04-11 Illumina Singapore Pte Ltd Algoritmo de secuenciación
WO2025255494A1 (en) 2024-06-07 2025-12-11 Illumina, Inc. Identifying the origin of amplified rna molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006137734A1 (en) * 2005-06-23 2006-12-28 Keygene N.V. Improved strategies for sequencing complex genomes using high throughput sequencing technologies

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0960215A1 (en) * 1997-01-15 1999-12-01 Brax Genomics Limited Nucleic acid sequencing
JP4403069B2 (ja) * 2002-08-12 2010-01-20 独立行政法人理化学研究所 クローニングおよび分析のためのｍＲＮＡの５’末端の使用方法
JP4413017B2 (ja) * 2004-01-15 2010-02-10 独立行政法人理化学研究所 クラスタリングプログラムおよびクラスタリング装置
MX2009000044A (es) * 2006-06-30 2009-01-23 Ambit Bios Corp Etiqueta de acido nucleico detectable.
EP2513333B1 (en) 2009-12-17 2013-10-02 Keygene N.V. Restriction enzyme based whole genome sequencing
CA2803940C (en) † 2010-06-30 2019-07-02 Bgi Genomics Co., Ltd. Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping
US9033273B2 (en) 2010-11-02 2015-05-19 Jonathan Edelson Integrated aircraft ground navigation control system
ES2986436T3 (es) 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
EP2820174B1 (en) † 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
PT2828218T (pt) 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014047556A1 (en) 2012-09-21 2014-03-27 The Broad Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006137734A1 (en) * 2005-06-23 2006-12-28 Keygene N.V. Improved strategies for sequencing complex genomes using high throughput sequencing technologies

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform;Kozich, James J.; Westcott, Sarah L.; Baxter, Nielson T.; 等.;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;20130930;第79卷(第17期);全文 *
Microbial diversity in the sputum of a cystic fibrosis patient studied with 16S rDNA pyrosequencing;Armougom, F.; Bittar, F.; Stremler, N.; 等.;《EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES》;20090930;第28卷(第9期);全文 *
The genome of Clostridium difficile 5.3;Darling, Aaron E.; Worden, Paul; Chapman, Toni A.; 等;《GUT PATHOGENS》;20140224;第6卷;Methods、Quality assurance和Initial findings部分 *

Also Published As

Publication number Publication date
DK3146070T5 (en) 2019-01-21
KR20230141873A (ko) 2023-10-10
JP6687605B2 (ja) 2020-04-22
KR102363284B1 (ko) 2022-02-15
EP3146070A1 (en) 2017-03-29
DK3146070T3 (en) 2019-01-07
EP3146070B1 (en) 2018-09-26
WO2015177570A1 (en) 2015-11-26
KR20220025213A (ko) 2022-03-03
KR102864537B1 (ko) 2025-09-24
US20210403991A1 (en) 2021-12-30
SG11201609754QA (en) 2016-12-29
ES2697804T3 (es) 2019-01-28
AU2015263055A1 (en) 2016-12-08
AU2015263055B2 (en) 2020-12-03
EP3146070B2 (en) 2024-07-31
CN116072218A (zh) 2023-05-05
KR20170012390A (ko) 2017-02-02
US20170183724A1 (en) 2017-06-29
CA2949925A1 (en) 2015-11-26
GB201409282D0 (en) 2014-07-09
JP2017517282A (ja) 2017-06-29
CN107002120A (zh) 2017-08-01
US11047002B2 (en) 2021-06-29
ES2697804T5 (es) 2024-11-25
KR102579902B1 (ko) 2023-09-18

Similar Documents

Publication Publication Date Title
CN107002120B (zh) 测序方法
Van Dijk et al. Ten years of next-generation sequencing technology
US11789906B2 (en) Systems and methods for genomic manipulations and analysis
AU2019250200A1 (en) Error Suppression In Sequenced DNA Fragments Using Redundant Reads With Unique Molecular Indices (UMIs)
CN110799653A (zh) 用于多重大规模平行测序的最佳索引序列
CN110832087A (zh) 用于多核苷酸样品的索引的通用短衔接子
CN107077537A (zh) 用短读测序数据检测重复扩增
CN103476946A (zh) 基于配对末端随机序列的基因分型
WO2012042374A2 (en) Method of determining number or concentration of molecules
EP3165612B1 (en) Method for counting number of nucleic acid molecules
CN104232760B (zh) 确定混合测序数据中读段的样本源的方法及装置
CN114555821B (zh) 检测与dna靶区域独特相关的序列
HK40086867A (zh) 测序方法
HK1235832A1 (en) Sequencing process
HK1235832B (en) Sequencing process

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20190307

Address after: New South Wales, Sydney, Australia

Applicant after: Langs Technology Co.,Ltd.

Address before: New South Wales Australia

Applicant before: University of Technology, Sydney

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20221114

Address after: Singapore, Singapore City

Patentee after: ILLUMINA SINGAPORE Pte. Ltd.

Address before: New South Wales, Sydney, Australia

Patentee before: Langs Technology Co.,Ltd.

TR01 Transfer of patent right