CN1069970C - Method for activating test-tube of maleic acid anhydride-phenylethylene copolymer - Google Patents
Method for activating test-tube of maleic acid anhydride-phenylethylene copolymer Download PDFInfo
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- CN1069970C CN1069970C CN 97100756 CN97100756A CN1069970C CN 1069970 C CN1069970 C CN 1069970C CN 97100756 CN97100756 CN 97100756 CN 97100756 A CN97100756 A CN 97100756A CN 1069970 C CN1069970 C CN 1069970C
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- test tube
- maleic anhydride
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Abstract
The present invention discloses an activation method for maleic anhydride styrene copolymer test tubes, which belongs to the field of biological chemistry. The present invention solves the problems of weak binding capacity of copolymers and antibodies, and low activation rate of the previous activation methods; the present invention is characterized in that a prepared maleic anhydride styrene copolymer solution is coated on a polystyrene test tube, and a sodium hydroxide solution is added in the coated test tube; the solution in the test tube is removed after being kept at room temperature; the maleic anhydride styrene copolymer test tube is activated through washing and drying. The activation rate of the maleic anhydride styrene test tubes produced in the activation method is increased by more than 30 times than the maleic anhydride styrene test tubes before activation; the present invention can be used for solid phase immune analysis of multiple antibodies and protein, and has the advantage of broad application range.
Description
--activation method of----styrol copolymer test tube belongs to biochemical field to the invention discloses a kind of maleic anhydride.
------styrol copolymer test tube is mainly used in the solid-phase immunoassay maleic anhydride, and its activation is meant that an antibody or other protein, polypeptides matter in the covalent bond mode, are fixed to the technology on polystyrene test tube (plate) surface; Promptly on test tube wall, introduce reactive group, make protein or antibody and its covalent bond, reach the purpose of sessile antibody.Known method mainly is to implant a kind of aminopolystyrene carrier at test tube wall at present, makes antibody and tube wall covalent bond by the bifunctional group coupling agent, thus sessile antibody; Its deficiency is that the activity of multipolymer own is not high, with protein, antibody binding capacity a little less than; Can not satisfy actual needs.
The object of the present invention is to provide that easy, activated test tube of a kind of method and protein, antibody binding capacity are strong, the maleic anhydride--activation method of----styrol copolymer test tube of applied range.
The object of the present invention is achieved like this:
Following steps of the present invention are formed:--the preparation of----styrol copolymer solution of (1) maleic anhydride; After maleic anhydride and styrene mixed by 2: 1 molecular proportions, add benzoyl peroxide and make catalyzer, additional proportion is 0.2% of a maleic anhydride weight; Place 75～80 ℃ of water-baths to heat, change over to immediately in the ice-water bath after thick material to be formed and the boiling, faint yellow solid congeals into; The cooling back adds the butanone that is equivalent to 8 times of volume of styrene makes it abundant dissolving, adds acetonitrile solution again and dilutes, and transfers to butanone concentration and reaches 15%～20%, promptly gets copolymer solution;
(2) coating polystyrene plastics test tube: get copolymer solution, inject the polystyrene test tube, leave standstill sucking-off complete soln after 10 seconds, the test tube tube wall after the coating leaves the layer of even co-polymer membrane, and room temperature condition is dry down;
(3) maleic anhydride--activation of----styrol copolymer test tube: the 0.1～1.0mol/L of the adding in vitro sodium hydroxide solution after coating, room temperature condition was placed 6～24 hours down, discard the solution in the pipe, with distilled water test tube is cleaned 5 times, under 50 ℃ of conditions, dry maleic anhydride--the styrol copolymer test tube promptly is activated.
The maleic anhydride that uses above-mentioned activation method to make--styrol copolymer test tube because the adding of sodium hydroxide solution makes multipolymer change water wettability into by hydrophobicity, has improved the ability with protein, antibodies; Multipolymer test tube conjugated protein after the activation, the ability of antibody have been brought up to the 131ug/ pipe by the preceding 4.0ug/ pipe of activation, and activation rate has been improved more than 30 times; The test tube that makes can be preserved 3 months under 4 ℃ of conditions, can deposit 3 years under-20 ℃ of conditions; And can be applicable to the solid-phase immunoassay of multiple antibody such as polyclone, monoclonal, affinity element and protein, have the advantage of applied range.
Claims (1)
1. a maleic anhydride---the activation method of polymers test tube is characterized in that being made up of following steps:
(1) maleic anhydride---the preparation of polymers solution: after maleic anhydride and styrene mixed by 2: 1 molecular proportions, add benzoyl peroxide and make catalyzer, additional proportion is 0.2% of a maleic anhydride weight; Place 75～80 ℃ of water-baths to heat, change over to immediately in the ice-water bath after thick material to be formed and the boiling, faint yellow solid congeals into; The cooling back adds the butanone that is equivalent to 8 times of volume of styrene makes it abundant dissolving, adds acetonitrile solution again and dilutes, and transfers to butanone concentration and reaches 15～20%, promptly gets copolymer solution;
(2) coating polystyrene test tube: get copolymer solution, inject the polystyrene test tube, leave standstill sucking-off complete soln after 10 seconds, the test tube tube wall after the coating leaves the layer of even co-polymer membrane, and room temperature condition is dry down;
(3) maleic anhydride---the activation of polymers test tube: the 0.1～1.0mol/L of the adding in vitro sodium hydroxide solution after coating, room temperature condition was placed 6～24 hours down, discard the solution in the pipe, with distilled water test tube is cleaned 5 times, under 50 ℃ of conditions, dry maleic anhydride---the styrol copolymer test tube promptly is activated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97100756 CN1069970C (en) | 1997-02-21 | 1997-02-21 | Method for activating test-tube of maleic acid anhydride-phenylethylene copolymer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97100756 CN1069970C (en) | 1997-02-21 | 1997-02-21 | Method for activating test-tube of maleic acid anhydride-phenylethylene copolymer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1167262A CN1167262A (en) | 1997-12-10 |
| CN1069970C true CN1069970C (en) | 2001-08-22 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97100756 Expired - Fee Related CN1069970C (en) | 1997-02-21 | 1997-02-21 | Method for activating test-tube of maleic acid anhydride-phenylethylene copolymer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1069970C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101864408A (en) * | 2010-05-25 | 2010-10-20 | 中国科学院过程工程研究所 | Preparation method of nanometer fiber immobilization beta-D-galactosidase |
| GB201107737D0 (en) * | 2011-05-09 | 2011-06-22 | Univ Birmingham | Extraction from cells |
| CN104558352B (en) * | 2013-10-28 | 2018-02-23 | 中国石油化工股份有限公司 | Block polymer and preparation method thereof and the application in thick oil hydrothermal catalytic visbreaking and the method for thick oil hydrothermal catalytic visbreaking |
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1997
- 1997-02-21 CN CN 97100756 patent/CN1069970C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1167262A (en) | 1997-12-10 |
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