CN106995786A - A kind of reaping hook bacteria strain and its application - Google Patents
A kind of reaping hook bacteria strain and its application Download PDFInfo
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- CN106995786A CN106995786A CN201610936466.1A CN201610936466A CN106995786A CN 106995786 A CN106995786 A CN 106995786A CN 201610936466 A CN201610936466 A CN 201610936466A CN 106995786 A CN106995786 A CN 106995786A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/77—Fusarium
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Abstract
The invention belongs to technical field of bioengineering, a kind of reaping hook bacteria strain and its application are specifically disclosed.Its deposit number of sickle-like bacteria Fusarium sp. is accredited as CCTCC M 2016582 by ITS gene orders.Present invention utilization inactivates FP JCCW thalline and studies its absorption behavior to thallium, draws optimal adsorption condition：PH5.0, initial concentration 100mg/L, time of contact 90min, rotating speed 150r/min, biomass 2.5g/L.And its absorption more meets Langmuir Isothermal Models and second-order kinetic equation, is mono layer adsorption, based on chemisorbed.FT IR, SEM, XRD phenetic analysis shows, FP JCCW porous surface gaps, and its absorption to thallium relies primarily on hydroxyl, carbonyl on cell membrane etc., and thalline crystal structure changes before and after absorption.Thus infer, inactive microorganism thalline FP JCCW absorption thalliums are completed by Electrostatic Absorption and surface complexation.The inactive microorganism FP JCCW that the present invention is provided can be used as economic and environment-friendly, sustainability sorbent treatment heavy metal thallium pollution.
Description
Technical field
The invention belongs to technical field of bioengineering, it is related to microorganism and its application, and in particular to a kind of sickle-like bacteria
(Fusarium sp.) bacterial strain FP-JCCW, and its inactive microorganism as adsorbent processing heavy metal thallium pollution in should
With.
Background technology
As process of industrialization develops, the heavy metal process problem after extensive mining is increasingly highlighted.Heavy metal thallium
Toxicity is strong, is easily enriched in sulfide, is often migrated in mining area exploitation, and can enter human body with food chain, to human lives
There is potential threat.
For mining, particularly thallium ore deposit and troilite, effective processing of heavy metal is that can mining industry sustainable development
Key.Nowadays the processing of heavy metal, mostly based on physics and chemical method, but both are compared to microbiological treatment, exist
Cost height, long processing period, the defects such as secondary pollution are easily caused, and be difficult to handle substantial amounts of low concentration pollution.Microbiological treatment
It is current domestic very active research field, such as fungi of pseudomonad, height endurability, depth flocculating microbial microorganism
The research of absorption thallium all obtains preferable effect, and the application handled which show good heavy metal pollution may.However, at present
Research is mostly to adsorb thallium using active bacterial strain, and the absorption research on inactive microorganism is also very few, and it can ignore microorganism
The inhibitions such as the repulsion of heavy metal itself.
The content of the invention
There is provided a kind of reaping hook for the above-mentioned technological deficiency using existing method processing heavy metal thallium pollution presence by the present invention
Bacterium (Fusarium sp.) bacterial strain FP-JCCW, and by the use of the bacterial strain the micro- raw body of inactivation as economic and environment-friendly, sustainability
Sorbent treatment heavy metal thallium pollution.
To achieve the above object, the present invention is adopted the technical scheme that：
A kind of sickle-like bacteria (Fusarium sp.) bacterial strain, the bacterial strain is FP-JCCW, be inventor from the big Golconda in Shaoguan by
The excellent anti-thallium fungi filtered out in Pollution Plant.The preservation information of the bacterial strain (biomaterial) is：
Title：Fusarium sp.FP-JCCW；
Deposit number：CCTCC NO：M 2016582；
Depositary institution：China typical culture collection center (CCTCC)；
Preservation address：Wuhan University of Wuhan, China city, postcode 430072；
The preservation time：On October 20th, 2016.
The present invention identifies the classification of FP-JCCW bacterial strains by ITS gene orders.ITS gene PCR amplimers are：ITS1
(5 '-TCCGTAGGTGAACCTGCGG-3 ') and ITS4 (5 '-TCCTCCGCTTATTGATATGC-3 ').By its gene order with
Related strain sequence in ncbi database carries out BLAST contrasts, and FP-JCCW bacterial strains are subordinate to together with sickle-like bacteria Fusarium sp.
One branch, thus be named as Fusarium sp. the present invention gene order of FP-JCCW bacterial strains is had been filed on NCBI's
GENBANK databases, strain gene sequence accession number is KX349437.
With reference to the qualification result of ITS gene orders, FP-JCCW bacterial strains of the present invention have with sickle-like bacteria Fusarium sp.
There is high homology, thus be named as Fusarium sp.FP-JCCW.
FP-JCCW bacterial strains can be used for preparing a kind of adsorbent.
Further, a kind of adsorbent can be prepared using the FP-JCCW thalline of inactivation.The preparation method of this adsorbent
For：FP-JCCW bacterial strains are subjected to Liquid Culture, FP-JCCW mycelium are filtrated to get；Then mycelium is placed in baking oven and dried
It is dry；The mycelium of drying is pulverized, the adsorbent is obtained.
In some embodiments, the preparation method of above-mentioned adsorbent is further optimized for：FP-JCCW bacterial strains are subjected to liquid training
72h is supported, with 8 layers of filtered through gauze, FP-JCCW mycelium are obtained；Then mycelium is transferred in enamel tray, is placed in 80 DEG C of baking ovens
Middle progress baking 6h；The mycelium of drying is placed and pulverized in mortar, the adsorbent is obtained.
Adsorbent of the present invention, can handle heavy metal thallium dirty as economic and environment-friendly, sustainability biological adsorption material
Dye.
Compared with prior art, the present invention at least has following beneficial effect or advantage：
(1) present invention separation screening from the big Golconda pollution mining-area plant in Shaoguan obtains one plant has stronger resistance to thallium
Bacterial strain, contrasts homology through BLAST, is inferred as sickle-like bacteria and is accredited as Fusuraium.sp.FP-JCCW, accession number is
KX349437.FP-JCCW bacterial strains can carry out heavy metal thallium pollution as economic, efficient, environment-friendly biological adsorption material
Processing.It is application and the place of following thallium The present invention gives the absorption behavior of inactivation FP-JCCW bacterial strain heavy metal thalliums and mechanism
Reason provides more theoretical foundations.
(2) present invention specify that inactive microorganism FP-JCCW adsorbs the optimal adsorption condition of thallium：PH5.0, initial concentration
100mg/L, time of contact 90min, rotating speed 150r/min, biomass 2.5g/L.
(3) Langmuir the and Freundlich models of the invention by setting up and contrasting absorption, the suction of FP-JCCW bacterial strains
Attached process more meets Langmuir Isothermal Models, and it is mono layer adsorption to illustrate it, and possible adsorption process occurs mainly in cell
On wall.Absorption firsts and seconds kinetics equation is set up, it is found that second-order kinetics more meets adsorption process, illustrates that adsorption process is
Chemisorbed is dominated, it may occur however that surface complex reaction.
(4) infrared spectrum analysis is passed through ,-OH, C-H, C=O ,-NH in FP-JCCW bacterial strain adsorption processes2, the base such as C-O
Main contributions effect is rolled into a ball.Observed by SEM-EDS and X-ray diffraction analysis adsorb front and rear inactive microorganism FP-JCCW, inhaled
Cell surface before attached is unsmooth and has hole, and cell morphology has significant change after absorption, and has crystal generation.Phenetic analysis
Afterwards, more specify that FP-JCCW absorption is completed by Electrostatic Absorption and surface complexation.
Brief description of the drawings
Fig. 1 is the phylogenetic tree of FP-JCCW bacterial strains of the present invention.
During Fig. 2 is adsorption test described in embodiment, pH is to Tl+The influence curve of absorption.
During Fig. 3 is adsorption test described in embodiment, Tl+Influence curve of the initial concentration to absorption.
During Fig. 4 is adsorption test described in embodiment, influence curve of the time of contact to absorption.
During Fig. 5 is adsorption test described in embodiment, influence curve of the shaking speed to absorption.
During Fig. 6 is adsorption test described in embodiment, influence curve of the biomass to absorption.
During Fig. 7 is adsorption test described in embodiment, the Langmuir adsorption isotherm models of foundation.
During Fig. 8 is adsorption test described in embodiment, the Freundlich adsorption isotherm models of foundation.
During Fig. 9 is adsorption test described in embodiment, the first _ order kinetics equation of foundation.
During Figure 10 is adsorption test described in embodiment, the second-order kinetic equation of foundation.
Figure 11 is FP-JCCW bacterial strains absorption Tl of the present invention+Preceding A and B after absorption FTIR spectrum change pair
Than figure.
Figure 12 is FP-JCCW bacterial strains absorption Tl of the present invention+Preceding A and B after absorption ESEM change comparison diagram.
Figure 13 is FP-JCCW bacterial strains absorption Tl of the present invention+Preceding A and B after absorption energy spectrometer analysis figure.
Figure 14 is FP-JCCW bacterial strains absorption Tl of the present invention+Preceding A and B after absorption X-ray diffraction analysis figure.
Embodiment
Technical scheme is elaborated further below with reference to embodiment.
Embodiment 1：The separation and identification of FP-JCCW bacterial strains
(1) FP-JCCW bacterial strains are the resistant strains filtered out out of Shaoguan Dabaoshan Mine, specifically include following step
Suddenly：
(1) culture medium is prepared
PDA culture medium：Potato (peeling) 200g, glucose 20g, agar 20g, distilled water 1000mL, natural ph.
The specific process for preparation of PDA culture medium：By 200g peeling potatoes and eye, it is put into 1000mL's after being cut into small pieces
Boil to potato change is soft and, then with 8 layers of filtered through gauze, filtrate is re-poured into without rotten in pot in the lump in distilled water, plus
Enter agar and glucose uniform stirring and moisturizing is to 1000mL, be finally sub-packed in standby in 250mL triangular flask.
(2) domestication culture
Spore suspension is made in the endogenetic fungal bacterial strain of fresh cultured to logarithmic phase, takes 1mL spore suspensions to be applied to
In solid medium of the finite concentration containing thallium, it is placed in 25 DEG C of constant incubators and cultivates 72h, seen whether that bacterium colony is grown, such as
There is colony growth, then draw 1mL spore suspensions again and be inoculated into the fresh solid culture medium of higher thallium concentration, continue to cultivate,
Colony growth situation is observed, the minimum inhibitory concentration of thallium ion is determined.
(3) isolate and purify
Rinse the silt of the root, stem, leaf surface of herborization well with running water first, respectively in 95% ethanol and
Each sterilization 1min in 0.5% sodium hypochlorite, blotted after then being rinsed 3 times with sterile saline with sterilizing blotting paper it is standby,
And be aseptically cut into the fragment of 0.5 × 0.5cm sizes and be inoculated into fresh PDA culture medium, invert in 25 DEG C of perseverance
Static gas wave refrigerator in warm incubator.Cultivate after 3-7d, if fragment edge grows mycelia, with the good bacterium of transfer needle picking marginal growth
Silk be seeded on new solid medium, after the mycelia being newly inoculated with grows up to bacterium colony, then its edge of picking cultural hypha, so
Purify repeatedly, the bacterial strain purified.
(2) taxonomic identification is carried out to FP-JCCW bacterial strains using ITS gene sequencing method.
ITS gene PCR amplimers are：ITS1 (5 '-TCCGTAGGTGAACCTGCGG-3 ') and ITS4 (5 '-
TCCTCCGCTTATTGATATGC-3’)。
Amplification program is：95 DEG C of pre-degenerations 3min, 95 DEG C of denaturation 30s, 55 DEG C of annealing 40s, 72 DEG C of extension 60s, carry out 34
Individual circulation, 72 DEG C of extension 10min.
Through Shanghai life work sequencing after gene amplification product purifying, gained sequence is entered with existing ITS sequence in GENBANK
Row BLAST comparative analyses, Multiple sequence alignments are carried out with MEGA5.2, and NJ (Neighbor- are built using Kimura-2 models
Joining) systematic evolution tree.
The DNA of FP-JCCW bacterial strains is entered into performing PCR amplification, and by the related strain in its gene order and ncbi database
Sequence carries out BLAST contrasts.As a result show, the bacterial strain is subordinate to same branch with sickle-like bacteria Fusarium sp., thus is ordered
Entitled Fusarium sp., and with MEGA5.2 software buildings NJ (neighbor-joining) phylogenetic tree (Fig. 1), sequence
NCBI GENBANK databases are had been filed on, strain gene sequence accession number is KX349437.
Embodiment 2：The performance and the mechanism of action of FP-JCCW bacterial strain Adsorption of Heavy Metals thalliums
For the characterization of adsorption and preliminary mechanism of further clear and definite FP-JCCW bacterial strains heavy metal thallium, the present embodiment is with thallium
For research object, in characterization of adsorption of the microorganisms bacterial strain under different environmental conditions to thallium, Electronic Speculum, infrared spectrum point are utilized
The preliminary mechanism of the technique study bacterial strain Adsorption of Heavy Metals thalliums such as analysis.
(1) preparation of FP-JCCW bacterial strains adsorbent
FP-JCCW bacterial strains are subjected to Liquid Culture 72h, with 8 layers of filtered through gauze, FP-JCCW mycelium are obtained.Then by bacterium
Body is transferred in enamel tray, is placed in 80 DEG C of baking ovens and is carried out baking 6h.The mycelium of drying is placed and pulverized in mortar, is obtained
To inactive microorganism FP-JCCW (adsorbent).
Here the fluid nutrient medium of culture FP-JCCW bacterial strains is PDA liquid medium.PDA liquid medium is prepared as：
200g peeled potatoes, stripping and slicing is put into 1000ml distilled water and boils 30min, and filtered through gauze adds 20g glucose, and stirring and dissolving is fixed
Hold to 1000ml, be dispensed into conical flask, 121 DEG C of sterilizing 30min.
(2) Adsorption Test Method and result
(1) pH is to Sr2+The influence of absorption
It is 20mg/L in concentration, volume is 20ml Tl+In solution, 0.02g inactive microorganisms FP-JCCW is added.Bian Zhen
Swing side and nitric acid or sodium hydroxide is slowly added dropwise, pH to 3,4,5,6,7,8,9,30 DEG C of constant temperature 150r/min vibrations are adjusted respectively
60min.Solution 8000r/min centrifugation 5min after absorption, supernatant is cleared up, determine its Tl after centrifugation+Concentration.
PH is a material impact factor of microorganism adsorption.As seen from Figure 2, FP-JCCW bacterial strains adsorption process is with pH
Change.In pH<When 5, adsorbance increases with pH increase, it may be possible to because pH is too low so that water and hydrogen ion (H3O+) account for
According to adsorption site, inactive microorganism is hindered to Tl+Absorption.It is that degree of absorption reaches highest in pH=5, adsorbance is
120.9mg/g.Subsequent adsorbance increases and reduced with pH, and hydroxide colloid is likely to form in pH increase solution, and causes to inhale
Attached amount reduction.
(2) influence of the thallium initial concentration to absorption
It is respectively 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/L in concentration, volume is 20ml Tl+Solution,
Add 0.02g inactive microorganisms FP-JCCW, 30 DEG C of constant temperature 150r/min and vibrate 60min.Solution 8000r/min after absorption from
Heart 5min, clears up supernatant after centrifugation, determines its Tl+Concentration.
As seen from Figure 3, FP-JCCW adsorbance increases with the increase of initial concentration.The timing of liquor capacity one, metal
Ion concentration increases, and unit adsorbent adsorbance also increases therewith, it may be possible to because the adsorption site of adsorbent is not also by metal
Ion is fully occupied.As Tl in solution+When concentration is 100mg/L, adsorbance is up to 229.8mg/g.
(3) influence of the time of contact to absorption
It is 20mg/L in concentration, volume is 20ml Tl+In solution, 0.04g inactive microorganisms FP-JCCW is added.30℃
Constant temperature 150r/min vibrate respectively 10min, 20min, 30min, 40min, 50min, 60min, 70min, 80min, 90min,
100min、110min、120min.Solution 8000r/min centrifugation 5min after absorption, centrifuged supernatant is cleared up, and determines remaining
Tl+Concentration.
As seen from Figure 4, adsorbance all increases with the increase of time of contact, and balance is reached substantially to 90min.But connect
The tactile time is longer, and thallium ion is possible to be precipitated.Therefore 90min is optimum condition, and adsorbance is 116.8mg/g.
(4) influence of the shaking speed to absorption
It is 20mg/L in concentration, volume is 20ml Tl+In solution, 0.04g inactive microorganisms FP-JCCW is added.Respectively
30 DEG C of constant temperature oscillation 60min under 30r/min, 60r/min, 90r/min, 120r/min, 150r/min, 180r/min.Absorption
Solution 8000r/min centrifugation 5min afterwards, centrifuged supernatant is cleared up, and determines residue Tl+Concentration.
As seen from Figure 5, rotating speed is less than 150r/min, and adsorbance increases as rotating speed increases.Reached in 150r/min
Peak value is adsorbed, adsorbance is 117.3mg/g, and adsorbance is reduced suddenly in 180r/min afterwards.Rotating speed increases so that thallium ion
Reduce with the contact resistance of FP-JCCW bacterial strains, the chance contacted with adsorption site increases, therefore adsorbance increases.Can rotating speed mistake
It hurry up, the adsorption site of adsorbent may be caused to be destroyed, adsorbance reduction.
(5) influence of the biomass to absorption
It is 20mg/L in concentration, volume is 20ml Tl+In solution, be separately added into 0.5g/L, 1g/L, 2g/L, 3g/L,
4g/L, 5g/L inactive microorganism FP-JCCW, 30 DEG C of constant temperature 150r/min vibrate 60min.Solution 8000r/min after absorption from
Heart 5min, centrifuged supernatant is cleared up, and determines residue Tl+Concentration.
As seen from Figure 6, in the range of biomass is 0.5g/L-4g/L, adsorbance increases as biomass increases.
This is probably due to biomass increases, and adsorption site increases, and adsorbance is also increased.Biomass is more than after 2.5g/L, adsorbance
Increase with biomass and reduce.Probably due to ion concentration is not enough so that the absorption resistance increase of thallium ion and adsorption site.Inhale
Attached amount reaches when biomass is 1g/L is 117.6mg/g to the maximum, is certain because of concentration of metal ions, and biomass increases,
So unit adsorbent FP-JCCW adsorbance reduction.
(6) isothermal adsorption is studied
Influence test data according to initial concentration to adsorbent FP-JCCW, sets up Langmuir and Freundlich moulds
Type carries out analysis, and it is adsorbed, analysis result such as Fig. 7 and Fig. 8.
Can be seen that by Fig. 7 and Fig. 8, adsorbent FP-JCCW adsorption process closer to Langmuir adsorption isotherm models,
Adsorbent absorption thallium ion is based on mono layer adsorption.The isothermal coefficient Rs of Langmuir2For 0.9911, theory
Adsorption saturation amount B=129.87mg/g, absorption constant K=0.0246, substitute into Langmuir absorption empirical formulas Co/ W=1/KB+Co/
B, obtains Co/ W=3.2034+0.0077Co。
(7) adsorption dynamics adsorption kinetics research
Influence test data according to time of contact to adsorbent FP-JCCW, sets up first order kinetics and second-order kinetics
Equation carries out analysis, and it is adsorbed, analysis result such as Fig. 9 and Figure 10.
It can be seen that by Fig. 9 and Figure 10, adsorbent FP-JCCW adsorption process more meets pseudo-second order kinetic fitting, absorption
Process is based on chemisorbed.First order kinetics is fitted using 10-80min data, R2=0.9672, can preferably it retouch
State the starting stage of FP-JCCW absorption.Second-order kinetics describes the state that FP-JCCW adsorbs overall process, R2=0.9879.Cause
This, adsorption process is that, by physical absorption and chemisorbed collective effect, physical absorption acts predominantly on the starting stage of absorption, can
Can occur Electrostatic Absorption.And be chemisorbed on overall process and serve leading role, it may occur however that surface complexation.
(3) FP-JCCW bacterial strains are analyzed the SEM and FT-IR of thallium
(1) infared spectrum (FT-IR) is analyzed：The adsorbent 2mg of drying is taken to add 200mg spectroscopic pure KBr (mass ratioes
1:100) ground in agate mortar, be pressed into the thin slice of homogeneous transparent, sample is analyzed using Raman infrared spectrometer.
As seen from Figure 11, the FP-JCCW before and after adsorbing has significant change.3500cm-1Neighbouring absworption peak from
3385cm-1Have been moved to 3422cm-1, illustrate that hydroxyl (- OH) take part in reaction on cell membrane in the environment of thallium ion.2920cm-1
Neighbouring 2926cm-1And 2854cm-1The intensity of absworption peak is varied from, and illustrates that C-H bond (C-H) is joined in adsorption process fat carbochain
With reaction.1650cm-1Neighbouring absworption peak is from 1624cm-1Have been moved to 1653cm-1, positioned at amide Ⅰ, illustrate on amide groups
Carbonyl (C=O) performance is active, take part in adsorption reaction.1450cm-1Neighbouring 1458cm-1Absorption peak strength has significant change,
Illustrate the amino (- NH in the environment of thallium ion2) it take part in reaction.1088cm-1Neighbouring absorption peak strength also has significant change,
Explanation is that the carbon-oxygen bond (C-O) on cell membrane on carbohydrate or alcohol is shunk caused by vibration.FT-IR infrared analysis result tables
Mainly there are-OH, C-H, C=O ,-NH during bright FW-JCCW absorption thallium2, the group such as C-O take part in reaction.
(2) SEM-EDS is analyzed：After being pre-processed to the bacterial strain powder before and after absorption, FEI Quanta400FEG are placed in
Room temperature scanning under type environmental scanning electron microscope (FEI Co. of Czech), obtains the appearance structure of sample.
As seen from Figure 12, the scanning electron microscope (SEM) photograph before and after FW-JCCW absorption has significant change, shows inactive microorganism and thallium
Ion is reacted.It can be seen that antimicrobial surface is uneven before absorption, and have pore structure.Microorganism is scattered after absorption
Distribution, is around dispersed with some crystal, it may be possible to form the compound of thallium.
With the FP-JCCW after absorption before the absorption for being put into and handling well in energy depressive spectroscopy, SEM and EDS analyses are carried out,
Study the presence of inactive microorganism cell surface deposit thallium.
As seen from Figure 13, inactive microorganism is before absorption thallium ion, and cell surface has C, O, P, S, Cl and K peak.
After absorption, Tl absworption peak is occurred in that, while P, S, O element peak are considerably reduced, C is added, and can be shown that containing P, S, O
Group form chelate.Other K element peak is disappeared after absorption, occurs Na elements peak therewith, it is possible to absorption with it is competing
It is relevant that striving property suppresses potassium ion.
(3) XRD analysis：After the bacterial strain pressed powder before and after absorption, it is put into progress X-ray in D/max-2200/PC and spreads out
Penetrate analysis.
With the FP-JCCW after absorption before the absorption for being put into and handling well in X-ray diffractometer, XRD analysis are carried out.By scheming
14 can be seen that, FP-JCCW adsorbs preceding 2 θ and characteristic peak occurs in 8.28 °, 19.69 °, 28.35 °, 40.68 °, 47.22 °.After absorption,
It is 9.03 ° and 19.22 ° that peak at 8.28 °, 19.69 ° two, which is turned right and offset by a little, and its peak value all increases significantly, illustrates crystal
Structure changes, and FP-JCCW absorption may be by Electrostatic Absorption and surface complexation.
Further narration has been done to the present invention above in conjunction with embodiment, but the present invention is not limited to above-mentioned embodiment,
In the knowledge that one skilled in the relevant art possesses, it can also be made on the premise of present inventive concept is not departed from
Various change.
Claims (8)
1. a kind of sickle-like bacteria (Fusarium sp.) bacterial strain, it is characterised in that the bacterial strain is FP-JCCW, and the bacterial strain is in 2016
It is preserved in China typical culture collection center on October 20, in, deposit number is CCTCC M 2016582.
2. reaping hook bacteria strain according to claim 1, it is characterised in that the ITS gene PCR amplimers of the bacterial strain
For：ITS1 (5 '-TCCGTAGGTGAACCTGCGG-3 ') and ITS4 (5 '-TCCTCCGCTTATTGATATGC-3 ').
3. reaping hook bacteria strain according to claim 1, it is characterised in that gene of the bacterial strain in GENBANK databases
Sequence accession number is KX349437.
4. a kind of adsorbent, it includes bacterial strain according to claim 1.
5. adsorbent according to claim 4, it includes the deactivated strain prepared according to claim 1.
6. adsorbent according to claim 5, it is characterised in that the preparation method of the adsorbent is：By FP-JCCW bacterium
Strain carries out Liquid Culture, is filtrated to get FP-JCCW mycelium；Then mycelium is placed in baking oven and dried；By the mycelia of drying
Body is pulverized, and obtains the adsorbent.
7. adsorbent according to claim 6, it is characterised in that the preparation method of the adsorbent is：By FP-JCCW bacterium
Strain carries out Liquid Culture 72h, with 8 layers of filtered through gauze, obtains FP-JCCW mycelium；Then mycelium is transferred in enamel tray,
It is placed in 80 DEG C of baking ovens and carries out baking 6h；The mycelium of drying is placed and pulverized in mortar, the adsorbent is obtained.
8. the adsorbent according to any one of claim 4 to 7, it is used to handle heavy metal thallium pollution.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108033542A (en) * | 2017-12-08 | 2018-05-15 | 沈阳化工大学 | A kind of quick removal benzo（a）Pyrene（BaP）With the method for arsenic combined pollution waste water |
| CN109225151A (en) * | 2018-10-17 | 2019-01-18 | 广州大学 | Microorganism nano complex and the preparation method and application thereof |
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| CN108033542A (en) * | 2017-12-08 | 2018-05-15 | 沈阳化工大学 | A kind of quick removal benzo（a）Pyrene（BaP）With the method for arsenic combined pollution waste water |
| CN109225151A (en) * | 2018-10-17 | 2019-01-18 | 广州大学 | Microorganism nano complex and the preparation method and application thereof |
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