CN106995506A - A kind of method of the solvent of abundant dissolving liquaemin - Google Patents
A kind of method of the solvent of abundant dissolving liquaemin Download PDFInfo
- Publication number
- CN106995506A CN106995506A CN201710337508.4A CN201710337508A CN106995506A CN 106995506 A CN106995506 A CN 106995506A CN 201710337508 A CN201710337508 A CN 201710337508A CN 106995506 A CN106995506 A CN 106995506A
- Authority
- CN
- China
- Prior art keywords
- supernatant
- precipitation
- liquaemin
- sodium chloride
- adds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of method of the solvent of abundant dissolving liquaemin.Key step has enzymolysis, through adding sodium chloride, adjusts pH, heats, and the process operations such as enzymolysis obtain liquaemin extract solution；Oxidation, adds sodium hydroxide regulation pH, then adds hydrogen peroxide and is aoxidized；Then sodium chloride is added, is heated up, pH is adjusted, when stirring plus ethanol, precipitation ethanol precipitation is carried out, sodium chloride is added, heating adjusts pH, when stirring plus ethanol, is precipitated；Dehydration, supernatant is suctioned out, sediment adds absolute ethyl alcohol while stirring, is stood.The precipitation heating of supernatant, centrifugal drying will be removed.
Description
Technical field
Art of the present invention be field of biological pharmacy, more particularly to it is a kind of it is abundant dissolving liquaemin solvent method.
Background technology
Liquaemin is a kind of biochemical drug extracted from pig intestinal mucosa, is irreplaceable, redemption life in operation
, market can not be out of stock choice drug.The current country is isolated and purified in the technology of liquaemin, a large amount of organic molten using ethanol etc.
Agent such as is precipitated and is dehydrated at the operation.In order to reduce dissolvent residual so that the time being finally dried lengthens, increase technique is used
When.This method technique is simple, mild condition, produces safer, not only dissolvent residual content is low, and can improve liquaemin receipts
Rate, and improve liquaemin purity.It is a kind of technique that can carry out large-scale industrial production.
The content of the invention
, can not only using the extracting method it is an object of the invention to provide a kind of method of the solvent of abundant dissolving liquaemin
Dissolvent residual is reduced, and cost can be reduced, product quality is improved, improves production efficiency.The purpose of the present invention by enzymolysis,
Through adding sodium chloride, pH is adjusted, is heated, the process operations such as enzymolysis obtain liquaemin extract solution；Oxidation, adds sodium hydroxide regulation
PH, then adds hydrogen peroxide and is aoxidized；Ethanol precipitation, adds sodium chloride, heating adjusts pH, when stirring plus ethanol, carries out
Precipitation；Dehydration：Supernatant is suctioned out, sediment adds absolute ethyl alcohol while stirring, stood；The precipitation for removing supernatant is added
Heat, centrifugal drying;Sodium chloride mass fraction is 1.5%, adjusts pH to 7.0 for 3% hydrochloric acid solution with concentration, is warming up to 65 DEG C,
Pancreatin is added by the units of 35g/ hundred million;PH11 is adjusted for 35% sodium hydroxide solution with concentration, the volume for adding hydrogen peroxide is solution
The 5% of volume;By 7% plus sodium chloride of liquor capacity, pH to 7 is adjusted with 4% hydrochloric acid solution, temperature is heated to 65 DEG C, ethanol
Precipitating concentration is 80 degree；Time of repose is 7 hours;Add absolute ethyl alcohol make alcoholic strength be 95 degree, time of repose for 36 hours with
On；Siphon removes supernatant, and the precipitation for removing supernatant is heated into 65 DEG C, is transferred in centrifuge and centrifuges.
Specific embodiment
Technical scheme for a better understanding of the present invention, makees further detailed to the present invention with reference to specific embodiment
Describe in detail bright.
Enzymolysis, through adding sodium chloride, adjusts pH, heats, and the process operations such as enzymolysis obtain liquaemin extract solution；Oxidation, plus
Enter sodium hydroxide regulation pH, then add hydrogen peroxide and aoxidized；Ethanol precipitation, adds sodium chloride, and heating adjusts pH, side
Side plus ethanol are stirred, is precipitated；Dehydration：Supernatant is suctioned out, sediment adds absolute ethyl alcohol while stirring, stood；It will remove
The precipitation heating of supernatant, centrifugal drying;Sodium chloride mass fraction be 1.5%, with concentration for 3% hydrochloric acid solution adjust pH to
7.0,65 DEG C are warming up to, pancreatin is added by the units of 35g/ hundred million;PH11 is adjusted for 35% sodium hydroxide solution with concentration, peroxide is added
The volume for changing hydrogen is the 5% of liquor capacity;By 7% plus sodium chloride of liquor capacity, pH to 7 is adjusted with 4% hydrochloric acid solution, by temperature
Degree is heated to 65 DEG C, and ethanol precipitation concentration is 80 degree；Time of repose is 7 hours;Adding absolute ethyl alcohol makes alcoholic strength be 95 degree, quiet
The time is put for more than 36 hours；Siphon removes supernatant, and the precipitation for removing supernatant is heated into 65 DEG C, is transferred in centrifuge
Centrifugation.
The above described is only a preferred embodiment of the present invention, being not the limitation for making other forms to the present invention, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But it is every without departing from technical solution of the present invention content, the technical spirit according to the present invention is to above example institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.
Claims (1)
1. a kind of method of the solvent of abundant dissolving liquaemin, its feature is mainly reflected in following steps：Enzymolysis, through adding chlorination
Sodium, adjusts pH, heats, and the process operations such as enzymolysis obtain liquaemin extract solution；Oxidation, adds sodium hydroxide regulation pH, Ran Houjia
Enter hydrogen peroxide to be aoxidized；Ethanol precipitation, adds sodium chloride, and heating adjusts pH, when stirring plus ethanol, precipitated；It is de-
Water：Supernatant is suctioned out, sediment adds absolute ethyl alcohol while stirring, stood；The precipitation heating of supernatant will be removed, centrifugation is dry
It is dry;Sodium chloride mass fraction is 1.5%, adjusts pH to 7.0 for 3% hydrochloric acid solution with concentration, is warming up to 65 DEG C, single by 35g/ hundred million
Position adds pancreatin;PH11 is adjusted for 35% sodium hydroxide solution with concentration, the volume for adding hydrogen peroxide is the 5% of liquor capacity;
By 7% plus sodium chloride of liquor capacity, pH to 7 is adjusted with 4% hydrochloric acid solution, temperature is heated to 65 DEG C, ethanol precipitation concentration
For 80 degree；Time of repose is 7 hours;Adding absolute ethyl alcohol makes alcoholic strength be 95 degree, and time of repose is more than 36 hours；Siphon is removed
Supernatant is removed, the precipitation for removing supernatant is heated to 65 DEG C, is transferred in centrifuge and centrifuges.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710337508.4A CN106995506A (en) | 2017-05-15 | 2017-05-15 | A kind of method of the solvent of abundant dissolving liquaemin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710337508.4A CN106995506A (en) | 2017-05-15 | 2017-05-15 | A kind of method of the solvent of abundant dissolving liquaemin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106995506A true CN106995506A (en) | 2017-08-01 |
Family
ID=59434689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710337508.4A Withdrawn CN106995506A (en) | 2017-05-15 | 2017-05-15 | A kind of method of the solvent of abundant dissolving liquaemin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106995506A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113786793A (en) * | 2021-09-23 | 2021-12-14 | 潢川县鹏升畜产品有限公司 | Heparin sodium crude product extraction device and extraction method |
-
2017
- 2017-05-15 CN CN201710337508.4A patent/CN106995506A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113786793A (en) * | 2021-09-23 | 2021-12-14 | 潢川县鹏升畜产品有限公司 | Heparin sodium crude product extraction device and extraction method |
| CN113786793B (en) * | 2021-09-23 | 2022-08-02 | 潢川县鹏升畜产品有限公司 | Heparin sodium crude product extraction device and extraction method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103145878B (en) | A kind of ultralow point of heparin sodium technical study | |
| CN103030715B (en) | Method for separating purified heparin sodium | |
| CN106995506A (en) | A kind of method of the solvent of abundant dissolving liquaemin | |
| CN110872247A (en) | Xofluza sulfur-containing heterocyclic compound, intermediate thereof and preparation method | |
| CN108975433A (en) | A kind of recovery and treatment method of Iso-Propylalcohol wastewater | |
| CN103804522B (en) | A kind of method improving heparin sodium purity | |
| CN102603927B (en) | Method for preparing lignin and hemicellulose from wheat straws | |
| CN105399861A (en) | A method of reducing solvent residues in sodium chondroitin sulfate | |
| CN106674379A (en) | Method for reducing ethanol residues in sodium chondroitin sulfate | |
| RU2244008C1 (en) | Improved method for preparing sodium nucleate | |
| CN105399869A (en) | A method of reducing solvent residues in heparin sodium | |
| CN103755835A (en) | Method for stabilizing anti-Xa factor activity of refined heparin sodium by employing ethanol fractional precipitation process | |
| CN109929054A (en) | A kind of remaining method of reduction sodium chondroitin sulfate ethyl alcohol | |
| CN104448044B (en) | Method for decolorizing heparin sodium and lowering dermatan sulfate content | |
| CN106866663A (en) | A kind of process of praziquantel synthesis | |
| Ge et al. | Refolding of lysozyme in vitro assisted by colloidal thermosensitive poly (N‐isopropylacrylamide) brushes grafted onto the surface of uniform polystyrene cores | |
| CN107383237B (en) | Control method of chondroitin sulfate ethanol residue | |
| CN104292297A (en) | Method for comprehensively utilizing wastewater generated in production of starch through acid slurry technology | |
| CN103864701B (en) | The preparation method of 4-sulfanilamide-5-methoxyl group-6-chloropyrimide | |
| CN104724736B (en) | A kind of method that utilization ethylmaltol waste residue extracts magnesium chloride | |
| CN102321136B (en) | Preparation method for S-adenosine-L-methionine disulfate tosylate | |
| CN107557401B (en) | Preparation method of 2-thiophenecarboxylic acid | |
| CN106831499A (en) | A kind of preparation method of benzylidene camphor sulfonic acid | |
| CN110857474B (en) | Method for treating L-cysteine hydrochloride monohydrate | |
| JP2008179710A (en) | Method for producing low-molecular-weight hyaluronic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170801 |