CN106987589B - Method and container for long-term preservation of gene samples in cosmic space - Google Patents
Method and container for long-term preservation of gene samples in cosmic space Download PDFInfo
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- CN106987589B CN106987589B CN201710418086.3A CN201710418086A CN106987589B CN 106987589 B CN106987589 B CN 106987589B CN 201710418086 A CN201710418086 A CN 201710418086A CN 106987589 B CN106987589 B CN 106987589B
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 57
- 238000004321 preservation Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000007774 longterm Effects 0.000 title claims abstract description 19
- JJWKPURADFRFRB-UHFFFAOYSA-N carbonyl sulfide Chemical compound O=C=S JJWKPURADFRFRB-UHFFFAOYSA-N 0.000 title claims description 9
- 230000002068 genetic effect Effects 0.000 claims description 11
- 238000009413 insulation Methods 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 10
- 229910000881 Cu alloy Inorganic materials 0.000 claims description 7
- SBYXRAKIOMOBFF-UHFFFAOYSA-N copper tungsten Chemical compound [Cu].[W] SBYXRAKIOMOBFF-UHFFFAOYSA-N 0.000 claims description 7
- 210000004027 cell Anatomy 0.000 claims description 4
- 210000001519 tissue Anatomy 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- 210000003470 mitochondria Anatomy 0.000 claims description 2
- 210000004940 nucleus Anatomy 0.000 claims description 2
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 2
- 230000003389 potentiating effect Effects 0.000 claims 1
- 230000006641 stabilisation Effects 0.000 abstract description 2
- 238000011105 stabilization Methods 0.000 abstract description 2
- 230000005855 radiation Effects 0.000 description 6
- 238000013461 design Methods 0.000 description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 3
- 229910052721 tungsten Inorganic materials 0.000 description 3
- 239000010937 tungsten Substances 0.000 description 3
- 229910045601 alloy Inorganic materials 0.000 description 2
- 239000000956 alloy Substances 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000012611 container material Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052741 iridium Inorganic materials 0.000 description 1
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 229910052762 osmium Inorganic materials 0.000 description 1
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The invention discloses a method and a container for long-term preservation of gene samples in a space, wherein the gene samples containing biological gene information which are subjected to stabilization treatment are placed in the container, and the container enters the space along with a final stage of a space transportation system or a spacecraft to be preserved. The method can realize the ultra-long-time preservation of the perpetual level, and can realize the permanent preservation of biological gene information.
Description
Technical Field
The invention relates to the technical field of gene preservation, in particular to a method and a container for preserving a gene sample in a cosmic space for a long time.
Background
Gene preservation is widely applied to the gene detection industry, in the prior art, application publication No. CN101481733 discloses a DNA fixing method based on gel-fixed nucleic acid and application thereof, and the purpose of DNA normal-temperature long-term preservation is mainly sequencing; the application publication number CN106672441A discloses a preparation method for preserving DNA specimen at normal temperature and a packaging device, which is mainly used in the ground room temperature environment; application publication number CN101525613 discloses a method for preserving human DNA for funeral and interment, whose ground preservation cannot last for a long time; application publication number CN1299412 discloses a method for long-term preservation of DNA molecules and a package for implementing such long-term preservation, which, although mentioning the capsule preservation concept, is mainly used in a ground room temperature environment without radiation resistance.
Currently, there is no container or method for independently preserving genes in space outside the earth, except in space cabins and in specialized space experimental satellites.
Disclosure of Invention
In view of the above-described shortcomings, the present invention provides a method and container for long-term preservation of gene samples in a cosmic space.
To achieve the above object, a first object of the present invention is to provide a method for preserving a gene sample for a long period in a cosmic space, comprising:
placing the stabilized gene sample containing biological gene information into a container;
the container enters a space along with the final stage of a space transportation system or a spacecraft, so that the long-term preservation of the gene sample is realized.
As a further improvement of the present invention, the gene samples include biological tissues, cells, mitochondria, nuclei, DNA and RNA, and contain effective genetic information.
As a further development of the invention, the container is a sealed, thermally insulating, radiation-proof container.
A second object of the present invention is to provide a container for long-term preservation of a gene sample in a space, the container being cooperatively mounted on a final stage of a space transportation system or a rack of a spacecraft, comprising: stud, plug and sleeve;
a cavity is formed in the axis position of the stud;
the screw plug is screwed on the port of the cavity to form a closed sealing cavity; a closed heat insulation sleeve is placed in the sealing cavity, and a gene sample is contained in the heat insulation sleeve;
the screw sleeve is sleeved at the cavity end of the stud, and the container is installed on the bracket through the screw connection of the screw sleeve and the stud.
As a further improvement of the invention, the stud is a columnar structure made of W90 tungsten copper alloy;
the screw plug is a plug-shaped structure made of W90 tungsten copper alloy;
the screw sleeve is of a sleeve-shaped structure made of W90 tungsten copper alloy.
As a further improvement of the invention, the heat insulation sleeve is formed by buckling two cylindrical parts, and the cylindrical parts are cylindrical structures made of pure aluminum oxide.
As a further improvement of the invention, two heat insulation sleeves are arranged in the sealing cavity.
As a further improvement of the invention, the coating thickness of the container on the gene sample from outside to inside is more than 5mm.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a method and a container for long-term preservation of gene samples in a cosmic space, wherein the gene samples containing biological gene information through stabilization treatment are placed in the container and enter the cosmic space along with a final stage of a space transportation system or a spacecraft, so that the gene samples can be preserved for an ultra-long time in a perpetual level, and the permanent preservation of the biological gene information is realized.
Drawings
FIG. 1 is a block diagram of a container for long-term preservation of a genetic sample in a space, according to one embodiment of the present invention;
FIG. 2 is a schematic view of the stud of FIG. 1 in the A-direction;
FIG. 3 is a block diagram of the plug of FIG. 1;
FIG. 4 is a schematic view of the insert of FIG. 1 in the direction A;
fig. 5 is a block diagram of a thermal insulation sleeve for a container in accordance with one embodiment of the present invention.
In the figure:
1. a stud; 2. a screw plug; 3. a screw sleeve; 4. sealing the cavity; 5. a heat insulating sleeve; 6. and (3) a bracket.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
The invention is described in further detail below with reference to the attached drawing figures:
aiming at the problems existing in the prior art, the method has great significance on how to manufacture the storage capacity device and how to minimize the problem of utilizing space transportation resources; the invention provides a sealed, heat-insulating and radiation-proof container and a method for realizing long-term storage by carrying a spacecraft into a space.
The first object of the present invention is to provide a method for long-term preservation of gene samples in a space by placing stabilized biological components (gene samples) containing biological gene information (gene information, genetic data, genetic code) in a sealed, heat-insulated, radiation-proof container into the space along with the final stage of the space transportation system or spacecraft.
As shown in fig. 1 to 5, a second object of the present invention is to provide a container for long-term preservation of gene samples in a space, which is cooperatively mounted on a final stage of a space transportation system or a rack 6 of a spacecraft, comprising: stud 1, plug screw 2, sleeve screw 3 and heat insulation sleeve 5;
a cavity is formed in the axis position of the stud 1, and the axis of the cavity coincides with the axis of the stud 1; the screw plug 2 is screwed on the port of the cavity to form a closed sealing cavity 4; 1, 2 or more closed heat-insulating sleeves 5 are arranged in the sealed cavity 4, and the heat-insulating sleeves 5 contain stabilized biological gene information-containing (gene information, genetic data, genetic code) biological tissue, cells, DNA, RNA and other gene samples; the threaded sleeve 3 is sleeved at the cavity end of the stud 1, and the container is arranged on the bracket 6 through the threaded connection of the threaded sleeve 3 and the stud 1.
The invention provides a structural form of a container, and the container only needs to satisfy the following conditions: the sealed, heat-insulating and radiation-proof container for the whole-package gene sample is not limited to the container structure provided by the invention.
The quality of the container and the size of each part meet the design requirement of the space system bracket, and the performance of the container meets the design requirement of the space system; the mass of the vessel design was 142.6.+ -. 0.5g and the gene sample was about 0.1g.
As shown in FIG. 1, when the two containers are installed, the axial distance between the two containers is more than or equal to 23mm, so that the lower position of the wrench is facilitated.
As shown in figures 2, 3 and 4, the stud 1, the plug 2 and the sleeve 3 are made of W90 tungsten-copper alloy, and the components are as follows: cu10.4% W89.6%, density (g/cm 3 ): 16, conductivity (% IACS): 30, hardness (HRB. Gtoreq.): 98, softening temperature (. Gtoreq.): 900 ℃, tensile strength (Mpa): 330, performing a standard: GB/T8320-2003; the stud 1, the screw plug 2 and the screw sleeve 3 are not limited to W90, but can be made of gold, iridium, osmium, platinum, lead and the like.
As shown in FIG. 5, the heat insulation sleeve 5 of the present invention is composed of two cylindrical members, which are made of pure alumina; two heat-insulating sleeves 5 are placed in a sealing cavity formed by the stud and the screw plug, and the two heat-insulating sleeves 5 are pressed in the sealing cavity 1 through the screw plug 2.
The invention relates to a mounting mode of a container on a rocket, which comprises the following steps:
the DNA specimen is made into gene cabin load (the gene specimen is placed in a container) which is installed on a satellite support of the upper stage of a carrier rocket, and after the satellite is separated, the gene cabin load is carried into an uncontrollable floating state along with the last stage and the upper stage of the rocket which is kept in orbit. Alternatively, the gene-cabin load can be placed directly inside the space beacon and brought into the predetermined orbit in a standard manner like satellite launching.
The "container" of the present invention is a spatial radio beacon or storage compartment for storing a human inactivated DNA capsule. The container material innovatively uses a W90 alloy, i.e., an alloy of 90% metallic tungsten and 10% copper.
The equivalent aluminum thickness of tungsten is:
6.74 mm-6.84 mm;
i.e. 1mm tungsten is equivalent to 6.742-6.838 mm aluminum.
From the depth dose relationship curve, it can be further estimated that, in the case of GEO satellites >14mm Al (14 mm equivalent aluminum thickness) (data are not clear at larger thicknesses, the degree of improvement of the shielding effect should be less and less obvious), the cumulative radiation dose over 10 years is around 1e4rad (Si), and if 10000 years are considered, the total dose:
1e4*1000＝1e7rad(Si)。
it is emphasized here that the definition of rad, which refers to the radiant energy per unit mass of accumulated precipitate, is 100 reg/g=1 rad, where 1 reg=10 -7 J, g is the mass unit gram, so that the index is irrelevant to the real weight and the volume of the DNA sample; the cumulative radiation dose of one thousand years will not cause complete failure of the gene sample in the chamber.
The influence factors of the preservation of the gene sample in the space are temperature and radiation, and the low temperature is convenient for the preservation of the gene sample because the temperature in the space is low; the most dominant factor affecting preservation of gene samples is radiation. The cumulative radiation quantity is related to the material and thickness of the container, which is at least 2.8mm in thickness in order to achieve a thousand years of sealing; in order to ensure the perpetual preservation of genes, the invention designs the container to wrap the gene samples from outside to inside to have the wrapping thickness of more than 5mm.
The invention realizes the perpetual-level superlong-time preservation of biological gene information by putting the biological components (gene samples) which are stabilized and contain biological gene information (gene information, genetic data, genetic code) such as biological tissues, cells, DNA, RNA and the like into a sealed, heat-insulating and radiation-proof container along with the last stage of a space transportation system or a spacecraft entering a cosmic space, thereby becoming the reality of permanently preserving the biological gene information and mainly solving the problem that the perpetual-level long-time preservation of samples cannot be achieved by various methods in the past. On one hand, the service plan for recycling the value of the space launching waste part is completed, on the other hand, the contents such as space news information, knowledge, encyclopedia and the like are provided for non-space professionals of the general masses of China through the cooperation propaganda of the first party and the second party, the space interests of the teenagers and the masses of China are cultivated, the space knowledge education and the space spirit spreading are promoted, the national scientific culture quality is improved, the culture of talents of the Chinese space is promoted, and the development of the Chinese space is assisted.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A method for long-term preservation of a gene sample in a cosmic space, comprising:
placing the stabilized gene sample containing biological gene information into a container;
the container enters a space along with the final stage of a space transportation system or a spacecraft, so that long-term preservation of the gene sample is realized; wherein,,
the container is cooperatively arranged on a final stage of an aerospace transport system or a bracket of a spacecraft, and comprises: stud, plug and sleeve; a cavity is formed in the axis position of the stud; the screw plug is screwed on the port of the cavity to form a closed sealing cavity; a closed heat insulation sleeve is placed in the sealing cavity, and a gene sample is contained in the heat insulation sleeve; the screw sleeve is sleeved at the cavity end of the stud, and the container is arranged on the bracket through the screw connection of the screw sleeve and the stud;
the stud is of a columnar structure made of W90 tungsten-copper alloy, the screw plug is of a plug-shaped structure made of W90 tungsten-copper alloy, and the screw sleeve is of a sleeve-shaped structure made of W90 tungsten-copper alloy;
the heat insulation sleeve is formed by buckling two cylindrical parts, and the cylindrical parts are of a cylindrical structure made of pure aluminum oxide;
the coating thickness of the container on the gene sample from outside to inside is more than 5mm.
2. The method of long-term preservation of a gene sample in space according to claim 1, wherein the gene sample comprises biological tissue, cells, mitochondria, nuclei, DNA and RNA and contains potent genetic information.
3. The method for long-term preservation of a genetic sample in space according to claim 1, wherein the container is a sealed, thermally insulated, radiation-proof container.
4. The method for long-term preservation of a genetic sample in space of claim 1 wherein two insulating sleeves are placed within the sealed cavity.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710418086.3A CN106987589B (en) | 2017-06-06 | 2017-06-06 | Method and container for long-term preservation of gene samples in cosmic space |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710418086.3A CN106987589B (en) | 2017-06-06 | 2017-06-06 | Method and container for long-term preservation of gene samples in cosmic space |
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| CN106987589A CN106987589A (en) | 2017-07-28 |
| CN106987589B true CN106987589B (en) | 2023-06-27 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672321A (en) * | 1993-04-19 | 1997-09-30 | Samco Scientific, Inc. | Biological specimen collection system |
| CA2710372A1 (en) * | 2008-01-28 | 2009-08-06 | Prionics Ag | Container for the preparation, preservation and storage of biological samples using a drying agent |
| CA2794234A1 (en) * | 2010-03-25 | 2011-09-29 | Abon Biopharm (Hangzhou) Co., Ltd. | Detection device for detecting analytes in liquid specimen |
| CN105109708A (en) * | 2015-08-31 | 2015-12-02 | 北京航天长征飞行器研究所 | Thermal control method of spatial aircraft |
| CN204971364U (en) * | 2015-09-08 | 2016-01-20 | 深圳市华晨阳科技有限公司 | Cell collector for genetic test |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040179977A1 (en) * | 2003-03-10 | 2004-09-16 | Cem Corporation | Controlled Pressure Release Vessel for Microwave Assisted Chemistry |
| US20110295611A1 (en) * | 2010-05-30 | 2011-12-01 | Gelu Comanescu | Method, process and apparatus for preserving biological materials and information concerning biological materials for the use of descendants and future generations. |
-
2017
- 2017-06-06 CN CN201710418086.3A patent/CN106987589B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672321A (en) * | 1993-04-19 | 1997-09-30 | Samco Scientific, Inc. | Biological specimen collection system |
| CA2710372A1 (en) * | 2008-01-28 | 2009-08-06 | Prionics Ag | Container for the preparation, preservation and storage of biological samples using a drying agent |
| CA2794234A1 (en) * | 2010-03-25 | 2011-09-29 | Abon Biopharm (Hangzhou) Co., Ltd. | Detection device for detecting analytes in liquid specimen |
| CN105109708A (en) * | 2015-08-31 | 2015-12-02 | 北京航天长征飞行器研究所 | Thermal control method of spatial aircraft |
| CN204971364U (en) * | 2015-09-08 | 2016-01-20 | 深圳市华晨阳科技有限公司 | Cell collector for genetic test |
Non-Patent Citations (2)
| Title |
|---|
| DNA测序进入太空时代;张迪;;创新时代(第02期);全文 * |
| 殖民外太空;奇闻怪事(02);第2-6页 * |
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