CN106978335A - A kind of making nucleic acid molecular hybridization device - Google Patents
A kind of making nucleic acid molecular hybridization device Download PDFInfo
- Publication number
- CN106978335A CN106978335A CN201710383838.7A CN201710383838A CN106978335A CN 106978335 A CN106978335 A CN 106978335A CN 201710383838 A CN201710383838 A CN 201710383838A CN 106978335 A CN106978335 A CN 106978335A
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- Prior art keywords
- nucleic acid
- rotation
- acid molecular
- molecular hybridization
- making nucleic
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- 238000009396 hybridization Methods 0.000 title claims abstract description 20
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 20
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 20
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 20
- 238000002474 experimental method Methods 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000010808 liquid waste Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108091027305 Heteroduplex Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of making nucleic acid molecular hybridization device, including base, the lower surface both sides of the base are provided with supporting leg, the appearance surface rear end of the base is installed with switch, the upper surface center of the base is provided with rotation slot, the inner upper end of the rotation slot is provided with upper limit position block, the inner bottom surface of the rotation slot is provided with the first screwed hole, the internal whorl of first screwed hole is connected with the first threaded rod, the inner rotation of the rotation slot is connected with moving block, the upper surface two ends of the moving block are provided with lower position block, the upper surface of the moving block is fixedly connected with rotation dish by support bar, the upper surface two ends of the rotation dish are hinged with reaction utensil by hinged seat, the rotation dish outer surface be provided with rail groove, the inside both ends of the surface of the rail groove are provided with guide groove, the inside of the guide groove is rotatably connected to bracket panel by guide pad.The making nucleic acid molecular hybridization device can greatly improve the practicality and high efficiency of hybrid device.
Description
Technical field
The present invention relates to nucleic acid molecules engineering device technique field, specially a kind of making nucleic acid molecular hybridization device.
Background technology
Stable heteroduplex DNA molecular is formed by Walson-Crick base pairings in complementary nucleotide sequence
Process is referred to as hybridization, and making nucleic acid molecular hybridization is a kind of basic molecular biology experiment, for example, be in the blood of detection patient
No to contain specific pathogen or tumour cell, it is necessary to carry out nucleic acid molecular hybridization experiments, making nucleic acid molecular hybridization device is exactly
Carry out the instrument used in this experiment.
But existing making nucleic acid molecular hybridization device also has some shortcomings part, such as the existing Eclectics when in use
Device when being stirred mostly be that rotation, waste of resource, then it is exactly reacted liquid waste processing to have are driven using single motor
Also it is cumbersome to compare and lose time, it is necessary to distinguish transfer processing using pipette, the efficiency of strong influence experiment, and
And can also increase the amount of labour.
The content of the invention
It is an object of the invention to provide a kind of making nucleic acid molecular hybridization device, with asking for solving to propose in above-mentioned background technology
Topic.
To achieve the above object, the present invention provides following technical scheme：A kind of making nucleic acid molecular hybridization device, including base,
The lower surface both sides of the base are provided with supporting leg, and the appearance surface rear end of the base is installed with switch, the base it is upper
Rotation slot is provided with centre of surface, the inner upper end of the rotation slot is provided with upper limit position block, and the inner bottom surface of the rotation slot is set
There is the first screwed hole, the internal whorl of first screwed hole is connected with the first threaded rod, and the inner rotation of the rotation slot connects
Moving block is connected to, the upper surface two ends of the moving block are provided with lower position block, and the upper surface of the moving block is solid by support bar
Surely rotation dish is connected with, the upper surface center of the rotation dish is provided with through hole, and the upper surface two ends of the rotation dish pass through hinge
Joint chair is hinged with front side of reaction utensil, the lower surface of the reaction utensil and is installed with before back-moving spring, the inside of the reaction utensil
End is provided with taper surface, and the outer surface of the rotation dish is provided with rail groove, and the inside both ends of the surface of the rail groove are provided with guide groove, institute
The inside for stating guide groove is rotatably connected to bracket panel by guide pad, and the front-end surface of the bracket panel is rolled by roller to be connected
In the inner side of rail groove, the lower surface of the bracket panel is threaded with the second threaded rod by the second screwed hole, described
Wedge is fixedly connected with rear side of the upper surface of bracket panel, the upper surface slide of the wedge is connected to the lower surface of reaction utensil
Rear side, the outer surface of the bracket panel is provided with big belt wheel, and the outer surface of the big belt wheel is rotatably connected to small pulley by belt,
The small pulley is fixedly connected with motor by axle, and the motor is fixedly installed on the outside of the upper surface of base, and the motor leads to
Oversampling circuit is series at switch.
It is preferred that, the upper surface slide of first threaded rod is connected to the lower surface center of moving block.
It is preferred that, the upper limit position block is slidably connected to the outer surface of lower position block.
It is preferred that, the reaction utensil at least six, and angularly it is distributed in the upper surface of rotation dish.
It is preferred that, the height of the wedge is not less than the height of hinged seat.
It is preferred that, the upper surface slide of second threaded rod is connected to the lower surface of rotation dish.
Compared with prior art, the beneficial effects of the invention are as follows：The making nucleic acid molecular hybridization device is turned by screw threads for fastening
Moving plate combination motor, can will stir and liquid waste processing is driven using common motor, compare and economize on resources, and pass through wedge shape
Block and back-moving spring coordinate, and realize that batch topples over waste liquid, efficiency comparison is high, can greatly improve the practicality and height of hybrid device
Effect property.
Brief description of the drawings
Fig. 1 is overall structure diagram of the invention；
Fig. 2 is moving block connection diagram of the invention；
Fig. 3 is bracket panel connection diagram of the invention；
Fig. 4 is the sectional view of the reaction utensil of the present invention.
In figure：1 base, 11 supporting legs, 12 switches, 13 rotation slots, 14 first screwed holes, 15 first threaded rods, 16 upper limits
Block, 2 moving blocks, 21 lower position blocks, 22 support bars, 23 rotation dish, 24 through holes, 25 hinged seats, 26 reaction utensils, 27 back-moving springs,
28 taper surfaces, 3 rail grooves, 31 guide grooves, 32 guide pads, 33 bracket panels, 34 rollers, 35 second screwed holes, 36 second threaded rods,
37 wedges, 4 big belt wheels, 41 belts, 42 small pulleys, 43 motors.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1-4 are referred to, the present invention provides a kind of technical scheme：A kind of making nucleic acid molecular hybridization device, including base 1, bottom
The lower surface both sides of seat 1 are provided with supporting leg 11, and the appearance surface rear end of base 1 is installed with switch 12, the upper surface center of base 1
Place is provided with rotation slot 13, and the inner upper end of rotation slot 13 is provided with upper limit position block 16, and upper limit position block 16 is slidably connected to lower position block
21 outer surface, can carry out spacing to moving block 2, and the inner bottom surface of rotation slot 13 is provided with the first screwed hole 14, the first screw thread
The internal whorl in hole 14 is connected with the first threaded rod 15, and the upper surface slide of the first threaded rod 15 is connected to the following table of moving block 2
Face center, is easy to regulation, and the inner rotation of rotation slot 13 is connected with moving block 2, and the upper surface two ends of moving block 2 are provided with lower limit
Position block 21, the upper surface of moving block 2 is fixedly connected with rotation dish 23 by support bar 22, and the upper surface center of rotation dish 23 is set
There is a through hole 24, the upper surface two ends of rotation dish 23 are hinged with reaction utensil 26 by hinged seat 25, reaction utensil 26 at least six, and
The upper surface of rotation dish 23 is angularly distributed in, fixed peace on front side of multiple experiments, the lower surface of reaction utensil 26 can be carried out simultaneously
Equipped with back-moving spring 27, the interior forward end of reaction utensil 26 is provided with taper surface 28, and the outer surface of rotation dish 23 is provided with rail groove 3, rail
The inside both ends of the surface of road groove 3 are provided with guide groove 31, and the inside of guide groove 31 is rotatably connected to bracket panel 33 by guide pad 32, auxiliary
The front-end surface of disk 33 is helped to roll the inner side for being connected to rail groove 3 by roller 34, the lower surface of bracket panel 33 passes through the
Two screwed holes 35 are threaded with the second threaded rod 36, and the upper surface slide of the second threaded rod 36 is connected to the following table of rotation dish 23
Bracket panel 33 and rotation dish 23, can so be fixed together by face, are easy to be stirred while rotating, use when side
Just, wedge 37 is fixedly connected with rear side of the upper surface of bracket panel 33, the height of wedge 37 is not less than the height of hinged seat 25,
Guarantee the rear end of reaction utensil 26 is too high, be easy to waste liquid to flow out, the upper surface slide of wedge 37 is connected to reaction utensil 26
Lower surface on rear side of, the outer surface of bracket panel 33 is provided with big belt wheel 4, and the outer surface of big belt wheel 4 is rotatably connected to by belt 41
Small pulley 42, small pulley 42 is fixedly connected with motor 43 by axle, and motor 43 is fixedly installed on the outside of the upper surface of base 1, electricity
Machine 43 is by circuit connected in series in switch 12.
The present invention is in the specific implementation：When in use, from model UB-03 motor 43, volume is smaller, and will
Switch 12 is connected in series in working power, then can be put into reaction solution in reaction utensil 26 and be reacted, it is necessary to when being stirred, can turn
Dynamic second threaded rod 36 holds out against the lower surface of rotation dish 23, so rotation dish 23 and bracket panel 33 can be fixed together, so
After 12 can be rotated by switch to start motor 43, so by the gearing of belt 41, can be led with bracket panel 33
It is dynamic, also it can just be rotated with rotation dish 23, while moving block 2 is rotated in rotation slot 13 is supported effect, at this moment
Upper limit position block 16 and lower position block 21 depart from, and also can just rotate and be stirred with reaction utensil 26, at this moment speed can be most
Low grade, prevents reaction solution from throwing away, when, it is necessary to dispose waste liquid, at this moment first motor 43 can stopping, then unclamp after completion of the reaction
Second threaded rod 36 departs from bracket panel 33 and rotation dish 23, and then rotating the first threaded rod 15 can push up moving block 2 upwards
Rise, such lower position block 21 is also just contacted with upper limit position block 16, can be limited the rotation of moving block 2, also be limited rotation dish
23 rotation, may then pass through switch 12 and starts motors 43, similarly the same to be rotated with bracket panel 33, at this moment bracket panel 33 with
Rotation dish 23 is relatively rotated, and such bracket panel 33 is rotated with wedge 37 in the upper surface of rotation dish 23, can be by reaction utensil
26 rear end is raised, and taper surface 28 is also with regard to horizontal positioned, and such waste liquid can just be flowed out by taper surface 28, at this moment can be made
The lower section of through hole 24 is placed on vessel and contains the village, it is more convenient, the convenience used can be greatly improved.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of making nucleic acid molecular hybridization device, including base (1), it is characterised in that：The lower surface both sides of the base (1) are provided with
Supporting leg (11), the appearance surface rear end of the base (1) is installed with switch (12), the upper surface center of the base (1)
Provided with rotation slot (13), the inner upper end of the rotation slot (13) is provided with upper limit position block (16), the inside of the rotation slot (13)
Bottom surface is provided with the first screwed hole (14), and the internal whorl of first screwed hole (14) is connected with the first threaded rod (15), described
The inner rotation of rotation slot (13) is connected with moving block (2), and the upper surface two ends of the moving block (2) are provided with lower position block
(21), the upper surface of the moving block (2) is fixedly connected with rotation dish (23) by support bar (22), the rotation dish (23)
Upper surface center is provided with through hole (24), and the upper surface two ends of the rotation dish (23) are hinged with reaction utensil by hinged seat (25)
(26), it is installed with front side of the lower surface of the reaction utensil (26) before back-moving spring (27), the inside of the reaction utensil (26)
End is provided with taper surface (28), and the outer surface of the rotation dish (23) is provided with rail groove (3), the inside two ends of the rail groove (3)
Face is provided with guide groove (31), and the inside of the guide groove (31) is rotatably connected to bracket panel (33) by guide pad (32), described
The front-end surface of bracket panel (33) rolls the inner side for being connected to rail groove (3), the bracket panel (33) by roller (34)
Lower surface the second threaded rod (36) is threaded with by the second screwed hole (35), on rear side of the upper surface of the bracket panel (33)
Wedge (37) is fixedly connected with, the upper surface slide of the wedge (37) is connected on rear side of the lower surface of reaction utensil (26),
The outer surface of the bracket panel (33) is provided with big belt wheel (4), and the outer surface of the big belt wheel (4) is rotated by belt (41) to be connected
There is small pulley (42), the small pulley (42) is fixedly connected with motor (43) by axle, and the motor (43) is fixedly installed in bottom
On the outside of the upper surface of seat (1), the motor (43) is by circuit connected in series in switch (12).
2. a kind of making nucleic acid molecular hybridization device according to claim 1, it is characterised in that：First threaded rod (15)
Upper surface slide is connected to the lower surface center of moving block (2).
3. a kind of making nucleic acid molecular hybridization device according to claim 1, it is characterised in that：The upper limit position block (16) is slided
It is connected to the outer surface of lower position block (21).
4. a kind of making nucleic acid molecular hybridization device according to claim 1, it is characterised in that：The reaction utensil (26) is at least
Six, and angularly it is distributed in the upper surface of rotation dish (23).
5. a kind of making nucleic acid molecular hybridization device according to claim 1, it is characterised in that：The height of the wedge (37)
Not less than the height of hinged seat (25).
6. a kind of making nucleic acid molecular hybridization device according to claim 1, it is characterised in that：Second threaded rod (36)
Upper surface slide is connected to the lower surface of rotation dish (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710383838.7A CN106978335B (en) | 2017-05-26 | 2017-05-26 | A kind of making nucleic acid molecular hybridization device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710383838.7A CN106978335B (en) | 2017-05-26 | 2017-05-26 | A kind of making nucleic acid molecular hybridization device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106978335A true CN106978335A (en) | 2017-07-25 |
| CN106978335B CN106978335B (en) | 2018-09-18 |
Family
ID=59342779
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710383838.7A Expired - Fee Related CN106978335B (en) | 2017-05-26 | 2017-05-26 | A kind of making nucleic acid molecular hybridization device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106978335B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006118420A1 (en) * | 2005-04-30 | 2006-11-09 | Jae Chern Yoo | Bio-disc, bio-driver apparatus, and assay method using the same |
| CN205534913U (en) * | 2016-04-28 | 2016-08-31 | 安徽三联学院 | Machinery support with adjustable |
| CN106282007A (en) * | 2016-09-22 | 2017-01-04 | 周江 | Nucleic acid molecular cross instrument |
| CN206799630U (en) * | 2017-05-26 | 2017-12-26 | 胥振国 | A kind of making nucleic acid molecular hybridization device |
-
2017
- 2017-05-26 CN CN201710383838.7A patent/CN106978335B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006118420A1 (en) * | 2005-04-30 | 2006-11-09 | Jae Chern Yoo | Bio-disc, bio-driver apparatus, and assay method using the same |
| CN205534913U (en) * | 2016-04-28 | 2016-08-31 | 安徽三联学院 | Machinery support with adjustable |
| CN106282007A (en) * | 2016-09-22 | 2017-01-04 | 周江 | Nucleic acid molecular cross instrument |
| CN206799630U (en) * | 2017-05-26 | 2017-12-26 | 胥振国 | A kind of making nucleic acid molecular hybridization device |
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| Publication number | Publication date |
|---|---|
| CN106978335B (en) | 2018-09-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180528 Address after: 238000 Hefei, Career Technical College, Chaohu, Hefei, Anhui Applicant after: HEFEI TECHNOLOGY College Address before: 230000 Hefei, Career Technical College, Chaohu, Hefei, Anhui Applicant before: Xu Zhenguo |
|
| CB03 | Change of inventor or designer information |
Inventor after: Xu Zhenguo Inventor after: Pei Zhengfeng Inventor after: Cai Yuhua Inventor after: Fan Gaofu Inventor after: Liu Xiushu Inventor after: Wang Fenghua Inventor after: Xiang Min Inventor before: Xu Zhenguo Inventor before: Cai Yuhua Inventor before: Fan Gaofu Inventor before: Liu Xiushu Inventor before: Wang Fenghua Inventor before: Xiang Min |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190522 Address after: 050000 Room 517, 5th Floor, 2 Building, No. 136 Huanghe Avenue Science and Technology Center, Shijiazhuang High-tech Zone, Hebei Province Patentee after: Shijiazhuang Yigao Biotechnology Co.,Ltd. Address before: 238000 Hefei, Career Technical College, Chaohu, Hefei, Anhui Patentee before: HEFEI TECHNOLOGY College |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180918 |