CN106970048A - The method for detecting a variety of agricultural and veterinary chemicals residuals in food simultaneously based on the surface plasma resonance technology that regenerated liquid optimizes - Google Patents
The method for detecting a variety of agricultural and veterinary chemicals residuals in food simultaneously based on the surface plasma resonance technology that regenerated liquid optimizes Download PDFInfo
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
The invention discloses a kind of method that surface plasma resonance technology optimized based on regenerated liquid detects a variety of agricultural and veterinary chemicals residuals in food simultaneously, fixed including censorchip surface, standard liquid is prepared, tests regenerated liquid eluting power, set up standard working curve, quantitative detection and sensing chip regenerate, realize and detection simultaneously is carried out to the content of a variety of agricultural and veterinary chemicals residual components in same sample.Advantage of the invention is that utilizing antigen-antibody bond strength and the difference of regenerated liquid eluting power, efficiently realize highly sensitive, the detection simultaneously of a variety of agricultural and veterinary chemicals residuals in food, it is particularly suitable for use in the detection of small molecular weight material, compared with prior art, shorten the detection time of a variety of agricultural and veterinary chemicals residuals, testing cost is reduced, food safety detection efficiency is improved, popularization and application of the surface plasma resonance sensor in field of detection of food safety are promoted.
Description
Technical field
It is more particularly to a kind of to be total to based on the surface plasma that regenerated liquid optimizes the present invention relates to field of detection of food safety
The method that technology of shaking detects a variety of agricultural and veterinary chemicals residuals in food simultaneously.
Background technology
With the improvement of living standards, people are more concerned about the quality and safety situation of food.However, food is pacified at present
Full problem happens occasionally, particularly in terms of food middle peasant's residue of veterinary drug, and still very universal, this also becomes food security most
Big risk.The severe situation remained in face of agricultural and veterinary chemicals, rationally efficiently analysis detection is to ensure food safety, control agricultural and veterinary chemicals
The important step of residual.
Wherein, the biology developed based on surface plasma resonance technology (Surface Plasmon Resonance, SPR)
The features such as sensor is had without mark, highly sensitive, quick detection, is one of ideal tools of food safety detection.But,
The context of detection of low molecular weight substance, SPR still suffers from that signal to noise ratio is big, response is small, the low deficiency of sensitivity, and food middle peasant beast
The most of molecular weight of medicine residual is relatively low, such as sevin, streptomysin etc..In addition, the agricultural and veterinary chemicals more than one remained in food so that
Traditional detection process complexity is cumbersome, and the spr sensor of single sense channel can not be met while detecting a variety of agricultural and veterinary chemicals residual groups
The requirement divided.Therefore, in the urgent need to developing the novel detection method based on SPR technique, a variety of agricultural and veterinary chemicals residuals in food are realized
It is highly sensitive, quick, simultaneously detect.
The content of the invention
To solve problems of the prior art, the present invention provides a kind of surface plasma based on regenerated liquid optimization and is total to
Shake technology, the method that a variety of agricultural and veterinary chemicals residuals in food are detected while can be rapidly and efficiently.
The present invention is achieved by the following technical solutions：
A kind of surface plasma resonance technology optimized based on regenerated liquid detects a variety of agricultural and veterinary chemicals residuals in food simultaneously
Method, comprises the steps：
(1) fixation of censorchip surface coating antigen or antibody：The coating antigen or antibody of each agricultural and veterinary chemicals residual component are mixed
The volume for being bonded to mixed liquor is 50-150 μ L, and the concentration of each coating antigen or antibody in the mixed liquor is 5-20 μ g/
ML, recycles active ester method (EDC/NHS) that the mixed liquor is fixed on into the censorchip surface；
(2) standard liquid is prepared：The hydroxyethyl piperazine second thiosulfonic acid salt buffer for being 6-8 with 0.01-0.1mol/L, pH scope
Liquid (HBS buffer solutions) configures the standard sample storing solution of each agricultural and veterinary chemicals residual component, and the concentration of the standard sample storing solution is equal
For 0.1-1mg/mL；
The standard sample storing solution is measured, its corresponding standard stock solution is prepared；With HBS buffer solutions by the standard stock solution
The standard liquid of various concentrations is configured to, the phase of the standard liquid and excessive fixed concentration of various concentrations residual component to be measured is taken
Answer antibody or coating antigen to mix hatching, obtain the determinand mixed liquor of various concentrations；
(3) regenerated liquid eluting power is tested：Test HCl solution, NaOH solution, magnesium chloride solution and glycine-HCI
The elution profile for each residual component antibody of censorchip surface that solution is obtained for step (1)；
(4) standard working curve is set up：
The detection of one pack system：It is passed through the determinand mixed liquor of the various concentrations of 50-100 μ L steps (2) preparation, record
The response signal change of surface plasma resonance sensor, draws standard working curve, and carries out polynomial curve fitting, obtains
Obtain the equation of one pack system regression curve；The standard working curve and one pack system regression curve of other residual components are again measured successively
Equation；
Multi-analyte immunoassay：Standard liquid prepared by plurality of step (2) mixes hatching with excessive antibody or coating antigen, takes
Mixed liquor 50-100 μ L after hatching, sample introduction records overall response signal change, then according to the test knot of step (3)
Really, sample introduction is eluted the order grown from weak to strong according to regenerated liquid eluting power successively, recording responses value changes；Hatched according to various concentrations
The response of remaining antibody or coating antigen is drawn standard working curve and is fitted after rear mixed liquor and its corresponding regeneration, obtains many
The equation of component regression curve；
(5) quantitative detection：Detection simultaneously is carried out for unknown sample of the unknown content containing a variety of residual components, to described
Excessive antibody or coating antigen is separately added into the prepare liquid of unknown sample, sample introduction after mixing hatching records overall response
Change；Sample introduction is eluted the order grown from weak to strong according to regenerated liquid eluting power successively, each remaining antibody in memorization COMS clip surface or
The response value changes of coating antigen；The response of remaining antibody or coating antigen is substituted into the mark for the multi-analyte immunoassay that step (4) is obtained
Quasi- working curve and corresponding single group water content detection standard working curve, calculate the concentration value of each residual component；
(6) being passed through the glycine-HCI cushioning liquid that 0.01-0.02mol/L, pH value are 1.2-2.5 regenerates chip, then
Chip after life is used for subsequent detection.
Preferably, in step (2), described pH value is 7.4.
Preferably, in step (5), the mixing brooding time of standard sample storing solution and excessive antibodies or coating antigen is 5-
10 minutes.
The step of the above method in (1), when censorchip surface fix be the mixed liquor of coating antigen when, subsequently walking
Carry out just using antibody during mixing hatching in rapid.Conversely, the step of the above method in (1), being fixed when censorchip surface
When being the mixed liquor of antibody, carry out just using coating antigen during mixing hatching in subsequent step.
By the method for the present invention, the single channel of same chip can connect a variety of coating antigens simultaneously.Moreover, using different
The regenerated liquid of eluotropic strength carries out gradient elution to chip, while realizing that analyte detection to be measured regenerates with chip.According to single detection
Curvilinear equation and the curvilinear equation detected simultaneously, are detected while realizing a variety of determinands.The step of this method (4) and
(5) in, excessive antibody-solutions are passed through, using the mode of antibody binding chip, surface plasma body resonant vibration spectrometer sound are improved
It should be worth, it is adaptable to measure the less small-molecular-weight agricultural and veterinary chemicals residual component of response signal, its molecular weight can<1000Da.
The present invention uses spr sensor gradient regeneration method, and by regenerated liquid according to the order that grows from weak to strong, gradient elution is passed
The antibody of the different bond strengths of sensor surfaces absorption, the characteristics of being monitored in real time based on SPR, chip list after analysis regenerated liquid elution
Face antibody and the situation of response value changes, a variety of agricultural and veterinary chemicals residuals can be detected simultaneously.
Agricultural and veterinary chemicals to be measured are remained and premixed with excessive antibody by the method for the present invention, and unreacted antibody is with passing in mixed liquor
The coating antigen of sensor chip surface is specifically bound, and causes signal intensity；If target molecule is few in testing sample, mix
Remaining unreacted antibody is more in liquid so that the antibody of chip surface absorption is more, and now caused surface plasma is total to
Vibration response signal is bigger, on the contrary then smaller.Because the response value changes of antibody can reflect the change in concentration of target molecule to be measured,
And the molecular weight of antibody is larger, its caused response is changed greatly, therefore, and the highly sensitive detection of small-molecule substance can be achieved.
Moreover, when to a variety of target analyte detections to be measured, corresponding antibodies are combined with chip surface, overall sound is obtained by SPR
Answer value changes；According to the difference of adhesion between different antigen-antibodies, can by using varying strength elution solution gradient again
Raw mode, the order grown from weak to strong according to regenerated liquid is eluted, one by one recursion, finally by the real-time prison of spr sensor
Quick, the detection simultaneously of a variety of agricultural and veterinary chemicals residuals in food are realized in control.
The present invention provide based on surface plasma resonance sensor, by the way of gradient regenerates, detection food in
The method of a variety of low molecule amount agricultural and veterinary chemicals residuals, compared with prior art with advantages below：(1) present invention uses immunosupress
Method, by coating antigen modification in chip surface, substantially increase the service life of sensing chip, and solve SPR detection
The response of small molecular weight material is small, the low problem of sensitivity, realizes the highly sensitive detection of small molecule agricultural and veterinary chemicals；
(2) present invention is realized single in surface plasma resonance sensor by the way of regenerated liquid gradient elution
In sense channel, while detecting multiple harmful substances, compared to single material detection technique, sample detection efficiency is substantially increased,
Shorten sample detection time；
(3) this method is used, the purpose of the various detection of conventional one-channel spr sensor can not only be realized, and can be with
The flux of multi-channel detection is further improved, while improving the service efficiency of sensing chip, reduces and is harmful in detection food
The cost of thing.
Embodiment
The method of the present invention is described in detail with reference to specific embodiment.
Embodiment 1
Two kinds of typical food small molecular agricultural and veterinary chemicals of selection are detection object, respectively sevin and streptomysin.
Select the coating antigen that chip fixed member is two kinds of target molecules, i.e. bovine serum albumin(BSA)-sevin and cow's serum
Albumin-streptomysin.
The antibody of selection is mouse antibodies, i.e. sevin antibody and anti-streptomycin antibody
Concrete operation step is as follows：
(1) sensor surface coating antigen is fixed：Chip surface carboxyl groups are activated first with EDC/NHS methods；Then by west dimension
The coating antigen mixing of cause and streptomysin, it is 150 μ L to make cumulative volume, and two kinds of coating original contents are 20 μ g/mL, then with 30 μ L/
Min speed is passed through the sense channel of sensor, and the mixed liquor of two kinds of coating antigens is directly modified in sensor chip surface, most
Closed afterwards using pH for 8.5 monoethanolamine.
(2) standard liquid is prepared：Weigh 2 kinds of standard items 10mg of sevin and streptomysin respectively, be dissolved in 0.01mol/L,
PH value is 7.4 HBS buffer solutions, is settled to 10mL, as 1mg/mL Standard Reserving Solution；And 2 kinds of standard storages are measured respectively
Standby liquid, the standard stock solution for preparing 2 kinds of harmful substance residual components is respectively 100ng/mL；When one pack system is detected, 100 μ g/mL are taken
The μ L of sevin antibody 10, are mixed with the sevin standard stock solution of different volumes, are diluted to 100 μ L with HBS buffer solution mixed liquors, most
Obtain eventually sevin concentration in mixed liquor be followed successively by 0,5,10,20,30ng/mL；In a similar way, 10 μ g/mL strepto-s are taken
The plain μ L of antibody 10, are mixed with the streptomysin standard stock solution of different volumes, are diluted to 100 μ L with HBS buffer solution mixed liquors, final
Be followed successively by 0 to mixed liquor streptomycin concentration, 1,2.5,5,7.5,10ng/mL.
Two kinds of samples are detected simultaneously when, 10 μ L sevins antibody (100 μ g/mL), 10 μ L anti-streptomycin antibodies (10 μ g/mL) are taken
Sevin, streptomysin standard liquid with different volumes are mixed, and 100 μ L, wherein sevin are diluted to HBS buffer solution mixed liquors
Concentration is followed successively by 0,5,10,20,30ng/mL, streptomysin concentration is 0,1,2.5,5,7.5,10ng/mL, it is standby.
(3) screening of regenerated liquid：Selection is optimized to following actified solution：Different pH 0.01mol/L sweet ammonia
Acid-hydrochloric acid solution (Gly-HCl, pH value is respectively 1.2,1.5,2.0,2.5), concentration is respectively 0.015mol/L, 0.025mol/
L, 0.03mol/L, 0.035mol/L and 0.05mol/L NaOH solution, 5mol/L magnesium chloride solution (MgCl2) and
0.1mol/L HCl solution；Flow rate set is 30 μ L/min when sample in experiment is added, and is tested respectively, and observation is respective again
Come into force really, select optimal condition of protoplast isolation, and judge the power of two kinds of regenerated liquid eluting powers.
(4) Specification Curve of Increasing：
First, one pack system is detected：It is 0,5,10,20,30ng/mL sevins standard liquid and antibody mixed liquor to be passed through concentration
100 μ L, are measured using surface plasma resonance spectrum sensor, record surface plasma resonance sensor signal intensity, paint
Working curve processed, and polynomial curve fitting is carried out, obtain the equation of regression curve；Be passed through concentration for 0,1,2.5,5,7.5,
The μ L of 10ng/mL streptomysins mixed liquor 100, are measured using surface plasma resonance spectrum sensor, are recorded surface plasma and are total to
Vibration sensor signal intensity, drawing curve, and polynomial curve fitting is carried out, obtain the equation of regression curve.
Secondly, detected while two kinds of samples：The standard liquid of sevin and streptomysin, antigen are sufficiently mixed hatching,
Be passed through 100 μ L biased samples to be measured, the concentration of wherein sevin be testing sample concentration be 0,5,10,20,30ng/mL, record
The overall response signal change (T) of lower surface plasma resonance sensor；Then the weaker regenerated liquid of eluting power is utilized
(NaOH) eluted, wherein anti-streptomycin antibody is thoroughly washed away, sevin antibody still remains chip surface, thus exists
Certain response signal change (R)；With the change of sevin concentration in mixed liquor, response R can also change therewith, record west
The relation of denapon concentration of standard solution and its response R, drawing curve, and polynomial curve fitting is carried out, returned
Curvilinear equation.
(5) the glycine-HCI solution that 0.01mol/L, pH value are 1.2 is passed through, the sevin that chip surface is remained is resisted
Body is eluted, and completes sensing chip regeneration, standby.
(6) after sensing chip regeneration, actual unknown concentration testing sample is added and takes 10 μ L sevins antibody (100 μ g/
ML), 10 μ L anti-streptomycin antibodies (10 μ g/mL), mixing hatching；In the surface plasma resonance sensor being passed through, record is overall
Value changes (T) are responded, are then regenerated with NaOH solution, residual sevin antibody response (R) is obtained, R is substituted into its corresponding
Curvilinear equation, obtains sevin constituent content；Then sevin concentration be updated to the standard curve side during detection of its one pack system
Journey, obtains the response (C) of sevin；Streptomysin response is obtained using global response value T and sevin response C difference
(G) then, G substituted into standard curve during the single detection of streptomysin, so that it is determined that the content of streptomysin, test limit is up to ng/
ML grades.
(7) 0.01mol/L (pH is 1.2) glycine-HCI solution is passed through again, and the sevin that chip surface is remained is resisted
Body is eluted, and completes sensing chip regeneration, for measurement next time.
Sample detection, compared with existing surface plasma detection technique, detection efficiency are carried out according to the method for embodiment 1
More than 2 times are improved, testing cost reduces more than 2 times, and the time shortens more than 2 times, and reusable hundreds of times of chip is used
The method of the present invention solves conventional surface plasma resonance sensor single channel and is unable to the problem of Multiple detection, and can use
In the low molecule such as sevin amount (<1000Da) the detection of material.
Advantage of the invention is that using antigen-antibody bond strength and the difference of regenerated liquid eluting power, efficiently realizing
Highly sensitive, the detection simultaneously of a variety of agricultural and veterinary chemicals residuals, are particularly suitable for use in the detection of small molecular weight material, shorten a variety of in food
The detection time of agricultural and veterinary chemicals residual, reduces testing cost, improves food safety detection efficiency, promote surface plasma
Popularization and application of the resonance sensor in field of detection of food safety.
The present invention is described in detail for the accurate of narration and conveniently in embodiment by taking sevin, streptomysin as an example,
But determined the present disclosure applies equally to other agricultural and veterinary chemicals in food, the detection and analysis of such as Acetamiprid, terramycin, thus it is above-mentioned
Content is within the scope of the present invention, it should explanation, in the case where not departing from the core of the present invention, any simple
Deformation, modification or other skilled in the art can not spend the equivalent substitution of creative work each fall within the present invention
Protection domain.
Claims (3)
1. a kind of surface plasma resonance technology optimized based on regenerated liquid detects the side of a variety of agricultural and veterinary chemicals residuals in food simultaneously
Method, it is characterised in that comprise the steps：
(1) fixation of censorchip surface coating antigen or antibody：By the coating antigen or antibody of each agricultural and veterinary chemicals residual component mix to
The volume of mixed liquor is 50-150 μ L, and the concentration of each coating antigen or antibody in the mixed liquor is 5-20 μ g/mL, then
The mixed liquor is fixed on the censorchip surface using active ester method；
(2) standard liquid is prepared：The hydroxyethyl piperazine second sulphur phthalate buffer for being 6-8 with 0.01-0.1mol/L, pH scope is matched somebody with somebody
The standard sample storing solution of each agricultural and veterinary chemicals residual component is put, the concentration of the standard sample storing solution is 0.1-1mg/mL；
The standard sample storing solution is measured, its corresponding standard stock solution is prepared；With hydroxyethyl piperazine second sulphur phthalate buffer
The standard stock solution is configured to the standard liquid of various concentrations, the standard liquid and excess of various concentrations residual component to be measured is taken
Fixed concentration corresponding antibodies or coating antigen mixing hatching, obtain the determinand mixed liquor of various concentrations；
(3) regenerated liquid eluting power is tested：Test HCl solution, NaOH solution, magnesium chloride solution and glycine-HCI solution
The elution profile of each residual component antibody of censorchip surface obtained for step (1)；
(4) standard working curve is set up：
The detection of one pack system：It is passed through the determinand mixed liquor of the various concentrations of 50-100 μ L steps (2) preparation, recording surface
The response signal change of plasma resonance sensor, draws standard working curve, and carries out polynomial curve fitting, obtains single
The equation of component regression curve；Measure the standard working curve of other residual components and the side of one pack system regression curve successively again
Journey；
Multi-analyte immunoassay：Standard liquid prepared by plurality of step (2) mixes hatching with excessive antibody or coating antigen, takes hatching
Mixed liquor 50-100 μ L afterwards, sample introduction records overall response signal change, then according to the test result of step (3), according to
Sample introduction is eluted the order that regenerated liquid eluting power grows from weak to strong successively, recording responses value changes；After being hatched according to various concentrations
The response of remaining antibody or coating antigen is drawn standard working curve and is fitted after mixed liquor and its corresponding regeneration, obtains multicomponent
The equation of regression curve；
(5) quantitative detection：Detection simultaneously is carried out for unknown sample of the unknown content containing a variety of residual components, to described unknown
Excessive antibody or coating antigen is separately added into the prepare liquid of sample, sample introduction after mixing hatching records overall response value changes；
Sample introduction is eluted the order grown from weak to strong according to regenerated liquid eluting power successively, each remaining antibody in memorization COMS clip surface or coating antigen
Response value changes；The response of remaining antibody or coating antigen is substituted into the standard work for the multi-analyte immunoassay that step (4) is obtained
Curve and corresponding single group water content detection standard working curve, calculate the concentration value of each residual component；
(6) being passed through the glycine-HCI cushioning liquid that 0.01-0.02mol/L, pH value are 1.2-2.5 regenerates chip, after regeneration
Chip be used for subsequent detection.
2. according to the method described in claim 1, it is characterised in that：In step (2), described pH value is 7.4.
3. method according to claim 1 or 2, it is characterised in that：In step (5), standard sample storing solution resists with excessive
The mixing brooding time of body or coating antigen is 5-10 minutes.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110646381A (en) * | 2018-06-27 | 2020-01-03 | 北京中龙益诚科技有限公司 | Surface plasma resonance immunization method for detecting beta 2 receptor stimulant in pig urine |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001006236A1 (en) * | 1999-07-15 | 2001-01-25 | Gpc Biotech Ag | High throughput analysis of molecular interaction using surface plasmon resonance |
| US20120154818A1 (en) * | 2007-12-18 | 2012-06-21 | Plexera Llc | Spr apparatus with a high performance fluid delivery system |
| CN102628803A (en) * | 2012-04-17 | 2012-08-08 | 王利兵 | Method for detecting pesticide and veterinary medicament residues in foods based on surface plasma resonance technology |
| CN102645420A (en) * | 2012-04-17 | 2012-08-22 | 王利兵 | Method for detecting multiple harmful substance residual components in food based on surface plasma resonance technique |
-
2017
- 2017-02-17 CN CN201710087667.3A patent/CN106970048A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001006236A1 (en) * | 1999-07-15 | 2001-01-25 | Gpc Biotech Ag | High throughput analysis of molecular interaction using surface plasmon resonance |
| US20120154818A1 (en) * | 2007-12-18 | 2012-06-21 | Plexera Llc | Spr apparatus with a high performance fluid delivery system |
| CN102628803A (en) * | 2012-04-17 | 2012-08-08 | 王利兵 | Method for detecting pesticide and veterinary medicament residues in foods based on surface plasma resonance technology |
| CN102645420A (en) * | 2012-04-17 | 2012-08-22 | 王利兵 | Method for detecting multiple harmful substance residual components in food based on surface plasma resonance technique |
Non-Patent Citations (1)
| Title |
|---|
| YINQIANG XIA等: "Design of elution strategy for simultaneous detection of chloramphenicol and gentamicin in complex samples using surface plasmon resonance", 《BIOSENSORS AND BIOELECTRONICS》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110646381A (en) * | 2018-06-27 | 2020-01-03 | 北京中龙益诚科技有限公司 | Surface plasma resonance immunization method for detecting beta 2 receptor stimulant in pig urine |
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Application publication date: 20170721 |
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| WD01 | Invention patent application deemed withdrawn after publication |