CN106967756A - A kind of method that tomato of fermenting prepares insecticide - Google Patents

A kind of method that tomato of fermenting prepares insecticide Download PDF

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Publication number
CN106967756A
CN106967756A CN201710243609.5A CN201710243609A CN106967756A CN 106967756 A CN106967756 A CN 106967756A CN 201710243609 A CN201710243609 A CN 201710243609A CN 106967756 A CN106967756 A CN 106967756A
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fermentation
tomato
insecticide
water
commercially available
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CN201710243609.5A
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廖祥儒
窦欣
田乔鹏
蔡宇杰
管政兵
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Jiangnan University
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Jiangnan University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention provides a kind of technology for preparing biological source insecticide.Its process characteristic is that, using tomato as raw material, using being stored in China typical culture collection center, deposit number is：CCTCCNO：M2013283 bacillus amyloliquefaciens (Bacillus amyloliquefaciens) H47 and commercially available bacillus pumilus and commercially available Dipel mixed fermentation.Raw material adds strain 100mL after suitably smashing, and add water 10L, 25 DEG C -30 DEG C 3 12d of fermentation, and finished product is obtained after filtering.Preparation method of the present invention and during non-pollution safety it is reliable, the fermentation time cycle is short, and process procedure is few, simple to operation, can reduce investment outlay and operating cost.Compared with traditional chemical medicament, it has green safety, the advantage such as environment-friendly, and Promoting bacteria also has preferable disinsection prophylaxis and growth-promoting functions in itself, gathers around and has broad application prospects.

Description

A kind of method that tomato of fermenting prepares insecticide
Technical field
The present invention relates to one plant of bacillus amyloliquefacienses (Bacillusamyloliquefaciens) H47 of solution and short and small bud Spore bacillus and bacillus thuringiensis microbiological fermentation tomato prepare the production method of plant insecticide, belong to microorganism and fermentation skill Art field.
Background technology
Tomato (Solanum lycopersicum) is Solanaceae herbaceous plant, and complete stool is by cement glandular hairs.Stem is half orthostatic Or half tendril, easily lodging, high 0.7-1.0m or 1.0-1.3m.The branch ability of stem is strong, and easily raw adventitious root on stipes, stem is easy Lodging, contacts to earth, takes root, so tomato cutting propagation is easier to survive.Imparipinnate leaf or pinniform drastic crack, alternate；The long 5- of leaf 40cm；Leaflet irregular, differs in size, normal 5-9 pieces, and avette or Long Circle, long 5-7cm, front end is tapering, and edge has irregularly Sawtooth or sliver, it is local crooked, there is stalklet.Flower is hermaphrodite flower, yellow, self-pollination, compound raceme.3-9, flower, into side life Case umbrella inflorescence；Calyx 5-7 is split, and sliver lanceolar is harbored to linear during fruit；Corolla yellow, radial, 5-7 splits, and diameter is about 2cm；Stamen 5-7 roots, are born in a portion, and filigree is short, and flower pesticide partly polymerize shape, or is around in gynoecium in a cone；The Room of ovary 2 is at most Room, column cap head.Fruit is berry, berry oblate spheroid shape or near spherical, meat and succulence, crocus or cerise, smooth.Seed Flat, kidney shape, lark, mass of 1000 kernel 3.0-3.3g, life-span 3-4.Flower, fruiting period summer, autumn.Well developed root system, power of regeneration is strong, But most the distribution of root system is in 30-50cm soil layer.
Tomato seedling, which contains solanine and the nerve cell of the plain Central nervous system of tomato, has pharmacological action, disturbs elder brother " the information coding " of worm central nervous system, so as to influence its trophic behaviour.
Chinese Plants aboundresources, develops for plant insecticide and provides abundant insecticide active substance, and tool There is great potential；Chinese research exploitation medicinal plant is with a long history, and the research to Chinese medicine have accumulated rich experiences；Plant source is killed Worm agent is very wide in the development prospect of China, and important role will be played the part of in integrated pest management from now on.Utilize conjunction Suitable plant growth-promoting bacteria fermentation tomato, not only contributes to the extraction of physiological activator, is also less prone to cause the inactivation of active material, Promoting bacteria also has preferable disinsection prophylaxis and growth-promoting functions in itself.Be conducive to improving plant growth and strengthen insecticidal effect, favorably In the effective utilization of environmental protection and tomato plants in itself.
The content of the invention
In view of the foregoing defects the prior art has, the present invention is raw material using tomato plants, is protected with June 24th, 2013 China typical culture collection center is stored in, deposit number is：CCTCCNO：M2013283 bacillus amyloliquefaciens (bacterial strain is hereinafter referred to as by (Bacillus amyloliquefaciens) H47：Bacillus amyloliquefaciens H47CCTCCM2013283) and commercially available bacillus pumilus and commercially available Dipel fermentation prepare plant insecticide, favorably In improving plant growth and enhancing insecticidal effect, be conducive to environmental protection.
The fermentation technique scheme of invention：
1) strain prepares：By bacillus amyloliquefaciens H47CCTCCM2013283 and commercially available bacillus pumilus, commercially available Soviet Union Cloud gold bacillus is inoculated in seed culture medium respectively, in 30 DEG C, 250r/min concussion and cultivates 12h；The seed culture based component is pressed G/L is calculated as：Peptone 10, yeast extract 5, NaCl 10, remaining composition is water, and 121 DEG C of autoclaving 15min adjust pH to 7.0. Seed is done in mixing by a certain percentage.
2) zymotic fluid prepares：From superior in quality tomato, rinsed with water, 70% -75% alcohol-pickled 3min uses water Clean, it is stand-by after being blended through pulper.
3) fermentation ullage disappears and air duct sterilization：Fermentation ullage is carried out by instructions book to disappear.
4) zymotic fluid disappears in fact：Step 2 gained tomato is put into fermentation tank, supplement running water to final volume is 10L, by phase Close specification and carry out real elimination bacterium.
5) inoculated and cultured：When fermentation jar temperature is down to 37 DEG C, by step 1 gained seed by volume 1% inoculum concentration, It is seeded in the fermentation tank equipped with tomato, is cultivated 3-12 days under the conditions of 25-30 DEG C, throughput and the holding of technological requirement is set Tank pressure, sets other each culture parameters cultures.
6) discharge：Culture is finished, and releases zymotic fluid, is filtered, and gained zymotic fluid is insecticide finished product after filtering.The present invention Beneficial effect：
A kind of method of biological source insecticide is made the invention provides fermentation tomato.Preparation method of the present invention and during Non-pollution safety is reliable, and the fermentation time cycle is short, and process procedure is few, simple to operation, can reduce investment outlay and operating cost.With biography System chemical agent is compared, its tool green safety, the advantage such as environment-friendly, and Promoting bacteria also has preferable disinsection prophylaxis and rush in itself Raw effect, gathers around and has broad application prospects.
Specific implementation method：
With reference to embodiment, the present invention is described further.Following examples are only several specific realities of the present invention Example is applied, but the design concept of the present invention is not limited thereto, all changes for carrying out unsubstantiality to the present invention using this design, The behavior for invading the scope of the present invention should be belonged to.
Embodiment 1
1) strain prepares：By bacillus amyloliquefaciens H47CCTCCM2013283 and commercially available bacillus pumilus, commercially available Soviet Union Cloud gold bacillus is inoculated in seed culture medium respectively, in 30 DEG C, 250r/min concussion and cultivates 12h；The seed culture based component is pressed G/L is calculated as：Peptone 10, yeast extract 5, NaCl 10, remaining composition is water, and 121 DEG C of autoclaving 15min adjust pH to 7.0. By 1:1:Seed is done in 2 mixing.
2) zymotic fluid prepares：From superior in quality tomato, rinsed with water, 70% -75% alcohol-pickled 3min uses water Clean, it is stand-by after being blended through pulper.
3) fermentation ullage disappears and air duct sterilization：Fermentation ullage is carried out by instructions book to disappear.
4) zymotic fluid disappears in fact：Step 2 gained tomato is put into fermentation tank, supplement running water to final volume is 10L, by phase Close specification and carry out real elimination bacterium.
5) inoculated and cultured：When fermentation jar temperature is down to 37 DEG C, by step 1 gained seed by volume 1% inoculum concentration, It is seeded in the fermentation tank equipped with tomato, is cultivated 3-12 days under the conditions of 25 DEG C, the throughput of technological requirement is set and tank is kept Pressure, sets other each culture parameters cultures.
6) discharge：Culture is finished, and releases zymotic fluid, is filtered, and gained zymotic fluid is insecticide finished product after filtering.
Embodiment 2
1) strain prepares：By bacillus amyloliquefaciens H47CCTCCM2013283 and commercially available bacillus pumilus, commercially available Soviet Union Cloud gold bacillus is inoculated in seed culture medium respectively, in 30 DEG C, 250r/min concussion and cultivates 12h；The seed culture based component is pressed G/L is calculated as：Peptone 10, yeast extract 5, NaCl 10, remaining composition is water, and 121 DEG C of autoclaving 15min adjust pH to 7.0. By 1:2:Seed is done in 1 mixing.
2) zymotic fluid prepares：From superior in quality tomato, rinsed with water, 70% -75% alcohol-pickled 3min uses water Clean, it is stand-by after being blended through pulper.
3) fermentation ullage disappears and air duct sterilization：Fermentation ullage is carried out by instructions book to disappear.
4) zymotic fluid disappears in fact：Step 2 gained tomato is put into fermentation tank, supplement running water to final volume is 10L, by phase Close specification and carry out real elimination bacterium.
5) inoculated and cultured：When fermentation jar temperature is down to 37 DEG C, by step 1 gained seed by volume 1% inoculum concentration, It is seeded in the fermentation tank equipped with tomato, is cultivated 3-12 days under the conditions of 27 DEG C, the throughput of technological requirement is set and tank is kept Pressure, sets other each culture parameters cultures.
6) discharge：Culture is finished, and releases zymotic fluid, is filtered, and gained zymotic fluid is insecticide finished product after filtering.
Embodiment 3
1) strain prepares：By bacillus amyloliquefaciens H47CCTCCM2013283 and commercially available bacillus pumilus, commercially available Soviet Union Cloud gold bacillus is inoculated in seed culture medium respectively, in 30 DEG C, 250r/min concussion and cultivates 12h；The seed culture based component is pressed G/L is calculated as：Peptone 10, yeast extract 5, NaCl 10, remaining composition is water, and 121 DEG C of autoclaving 15min adjust pH to 7.0. By 1:2:Seed is done in 2 mixing.
2) zymotic fluid prepares：From superior in quality tomato, rinsed with water, 70% -75% alcohol-pickled 3min uses water Clean, it is stand-by after being blended through pulper.
3) fermentation ullage disappears and air duct sterilization：Fermentation ullage is carried out by instructions book to disappear.
4) zymotic fluid disappears in fact：Step 2 gained tomato is put into fermentation tank, supplement running water to final volume is 10L, by phase Close specification and carry out real elimination bacterium.
5) inoculated and cultured：When fermentation jar temperature is down to 37 DEG C, by step 1 gained seed by volume 1% inoculum concentration, It is seeded in the fermentation tank equipped with tomato, is cultivated 3-12 days under the conditions of 30 DEG C, the throughput of technological requirement is set and tank is kept Pressure, sets other each culture parameters cultures.
6) discharge：Culture is finished, and releases zymotic fluid, is filtered, and gained zymotic fluid is insecticide finished product after filtering.

Claims (7)

1. a kind of method that tomato of fermenting prepares biological source insecticide.It is characterized in that using tomato as raw material, adding strain fermentation system Standby insecticide, this method comprises the following steps：
1) strain prepares：By bacillus amyloliquefaciens H47CCTCCM2013283 and commercially available bacillus pumilus, commercially available Su Yun gold Bacillus is inoculated in seed culture medium respectively, in 30 DEG C, 250r/min concussion and cultivates 12h；The seed culture based component is based on g/L For：Peptone 10, yeast extract 5, NaCl 10, remaining composition is water, and 121 DEG C of autoclaving 15min adjust pH to 7.0.By one Seed is done in certainty ratio mixing.
2) zymotic fluid prepares：From superior in quality tomato, rinsed with water, 70% -75% alcohol-pickled 3min is eluted with water, It is stand-by after being blended through pulper.
3) fermentation ullage disappears and air duct sterilization：Fermentation ullage is carried out by instructions book to disappear.
4) zymotic fluid disappears in fact：Step 2 gained tomato is put into fermentation tank, supplement running water to final volume is 10L, by mutually speaking on somebody's behalf Bright book carries out real elimination bacterium.
5) inoculated and cultured：When fermentation jar temperature is down to 37 DEG C, by step 1 gained seed by volume 1% inoculum concentration, inoculation Into the fermentation tank equipped with tomato, cultivated 3-12 days under the conditions of 25-30 DEG C, the throughput of technological requirement is set and tank is kept Pressure, sets other each culture parameters cultures.
6) discharge：Culture is finished, and releases zymotic fluid, is filtered, and gained zymotic fluid is insecticide finished product after filtering.
2. the method that fermentation tomato according to claim 1 prepares biological source insecticide, it is characterized in that：The strain is independent Seed culture medium culture 12h mix by a certain percentage after as fermentation seed.
3. the method that fermentation tomato according to claim 1 prepares biological source insecticide, it is characterized in that：Strain (solution starch Bacillus H47CCTCCM2013283 and commercially available bacillus pumilus, commercially available Dipel) be with seed culture based formulas (g/L)：Peptone 10, yeast extract 5, NaCl 10, remaining composition is water, and 121 DEG C of autoclaving 15min adjust pH to 7.0.
4. the method that fermentation tomato according to claim 1 prepares biological source insecticide, it is characterized in that：Tomato processing method For：From superior in quality tomato, rinsed with water, 70% -75% alcohol-pickled 3min is eluted with water, after being blended through pulper It is stand-by.
5. the method that fermentation tomato according to claim 1 prepares biological source insecticide, it is characterized in that：The addition of strain For the 1% of fermentation system.
6. the method that fermentation tomato according to claim 1 prepares biological source insecticide, it is characterized in that：Raw material and strain are mixed Close uniform, 25-30 DEG C of fermentation temperature, fermentation time 3-12d.
7. the method that fermentation tomato according to claim 1 prepares biological source insecticide, it is characterized in that：The fermentation time cycle Short, process procedure is few, and the insecticide of preferable disinsection prophylaxis and growth-promoting functions can be obtained after fermentation.
CN201710243609.5A 2017-04-14 2017-04-14 A kind of method that tomato of fermenting prepares insecticide Pending CN106967756A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1265834A (en) * 1999-03-07 2000-09-13 宛吉斌 Process for preparing natural plant biological biocide and biological fertilizer
CN101697740A (en) * 2009-04-04 2010-04-28 冯子龙 Novel traditional Chinese medicine pesticide and production technology thereof
CN103451128A (en) * 2013-07-12 2013-12-18 江南大学 Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1265834A (en) * 1999-03-07 2000-09-13 宛吉斌 Process for preparing natural plant biological biocide and biological fertilizer
CN101697740A (en) * 2009-04-04 2010-04-28 冯子龙 Novel traditional Chinese medicine pesticide and production technology thereof
CN103451128A (en) * 2013-07-12 2013-12-18 江南大学 Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨柳、陈宇飞: "番茄茎叶提取物对萝卜蚜杀虫效果研究", 《生物技术通报》 *

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Application publication date: 20170721