CN106967592A - A kind of PCR pipe - Google Patents

A kind of PCR pipe Download PDF

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Publication number
CN106967592A
CN106967592A CN201710411444.8A CN201710411444A CN106967592A CN 106967592 A CN106967592 A CN 106967592A CN 201710411444 A CN201710411444 A CN 201710411444A CN 106967592 A CN106967592 A CN 106967592A
Authority
CN
China
Prior art keywords
pipe
lid
mouth
tube
plane mirror
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710411444.8A
Other languages
Chinese (zh)
Inventor
戴良
梁银针
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WUXI GUOSHENG BIOLOGICAL ENGINEERING Co Ltd
Original Assignee
WUXI GUOSHENG BIOLOGICAL ENGINEERING Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WUXI GUOSHENG BIOLOGICAL ENGINEERING Co Ltd filed Critical WUXI GUOSHENG BIOLOGICAL ENGINEERING Co Ltd
Priority to CN201710411444.8A priority Critical patent/CN106967592A/en
Publication of CN106967592A publication Critical patent/CN106967592A/en
Pending legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52Containers specially adapted for storing or dispensing a reagent
    • B01L3/523Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0689Sealing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • B01L2300/042Caps; Plugs

Abstract

The present invention is a kind of PCR pipe, its structure is to set concave plane mirror at the top of lid, the top surface of concave plane mirror is less than the top surface of lid, the arc steps of outwardly convex are set in the circumference exterior surface of lid bottom, the vertical section of arc steps is arch, fit circular arc step sets groove below the mouth of pipe on the inside of body, arc steps are interference fitted with groove, the vertical section of the mouth of pipe is isosceles trapezoid, the body first half is cylindrical tube, lower half is conical tube, conical tube is surrounded by taper tube wall, the bottom of conical tube is its bottom, the lower surface of its bottom is body bottom surface, body bottom surface is plane.Advantages of the present invention:Structure advantages of simple, it is easy to use, evaporation rate is reduced, the accuracy and experiment cycle period of experimental data is improved, reduces the cracking probability of tapered wall and conical bottom, prevent PCR instrument to be contaminated;Lid top surface sets concave plane mirror, is difficult to be worn, and scratches;Its bottom is designed as plane, convenient placement upright on the table.

Description

A kind of PCR pipe
Technical field
The present invention relates to a kind of PCR pipe.
Background technology
PCR is PCR, is a kind of Protocols in Molecular Biology for being used to amplify the specific DNA fragmentation of amplification, It is considered as the special DNA replication dna of in vitro, and PCR maximum feature is that micro DNA can be significantly increased.One in experiment As use PCR instrument, be denatured DNA using heating, make in the presence of polymerase it is single-stranded be copied into double-strand, and then reach that gene is answered The purpose of system.Wherein, PCR pipe is PCR important experimental apparatus.
The lid sealing structure of the PCR pipe of prior art easily causes evaporation rate too in the case of an experiment circulation It is high so that the experimental data of extraction is inaccurate, so as to influence medical diagnosis result.In experiment circulation, the main heating of PCR pipe Radiation areas be located at ttom of pipe taper surface on, the adapter angular compliance of this angle for requiring taper surface and PCR instrument it is consistent Property, it otherwise will influence to test cycle period, while the taper wall thickness of PCR pipe also has certain shadow to the efficiency of experiment circulation Ring, taper wall thickness, which is easily produced, splits pipe, and conical lower portion easily produces hole cracking, pollutes the adapter of PCR instrument.In addition, existing skill Plane is generally at the top of the lid of the PCR pipe of art, it is easy to be worn, scratch, lead to not clearly observe tubular body situation, It is very inconvenient and the bottom of body is generally not plane, it is impossible to upright on the table to place.
The content of the invention
Proposed by the present invention is a kind of PCR pipe, and its purpose aims to solve the problem that the lid sealing that existing PCR pipe is present, and body is opened The defect such as split, improve experiment cycle period, obtain accurately experiment effect.
The technical solution of the present invention:A kind of PCR pipe, its structure includes opening at the top of lid and body, its middle tube body There are the mouth of pipe and bottom lock, lid is plugged in the mouth of pipe, lid is the hollow tubular structure of top seal and bottom opening, lid Top sets concave plane mirror, and the top surface of concave plane mirror, which is less than in the top surface of lid, the circumference exterior surface of lid bottom, sets convex The arc steps risen, the vertical section of arc steps is that fit circular arc step sets groove, circular arc platform below the mouth of pipe on the inside of arch, body Rank is interference fitted with groove, and the vertical section of the mouth of pipe is isosceles trapezoid, and the body first half is cylindrical tube, and lower half is taper Body, conical tube is surrounded by taper tube wall, and the bottom of conical tube is its bottom, and the lower surface of its bottom is body bottom Face, body bottom surface is plane.
It is preferred that, the external diameter of the cylindrical tube of described body is that 8.0mm, thickness are 0.5mm, the bottom of conical tube External diameter be 3.0mm, angle of inclination be 18 °, thickness be 0.3mm.
It is preferred that, a diameter of 4.0mm of described concave plane mirror, thickness is 0.8mm, concave plane vertex face and lid top surface Distance be 0.5mm.
It is preferred that, a diameter of 5.1mm of described arc steps peak, groove top is from being 2.8mm with a distance from the mouth of pipe.
It is preferred that, a diameter of 6.5mm of the described mouth of pipe, highly it is 1.5mm.
It is preferred that, it is hemi-spherical cavities, a diameter of 2.6mm in described its bottom.
It is preferred that, described lid is formed in one with body and material is identical.
Advantages of the present invention:The present invention is simple and reasonable, and easy to use, lid is imported into body by mouth of pipe inclined-plane Groove in, make step in lid can interference in groove, reach good sealing effectiveness, reduce evaporation rate, improve reality Test the accuracy of data;The angle and wall thickness of body taper surface are reasonable in design, improve consistent with the cooperation of PCR instrument adapter Property, experiment cycle period is improved, the cracking probability of tapered wall and conical bottom is reduced, prevents PCR instrument to be contaminated;Lid top surface If concave plane mirror, it is difficult to be worn, scratches;Its bottom is designed as plane, convenient placement upright on the table.
Brief description of the drawings
Fig. 1 is the structural representation of PCR pipe of the present invention.
Fig. 2 is the open mode structural representation of PCR pipe of the present invention.
Fig. 3 is the open mode dimensional structure diagram of PCR pipe of the present invention.
1 in figure it is lid, 2 be concave plane mirror, 3 be arc steps, 4 be body, 5 be groove, 6 be conical tube, 7 is Body bottom surface, 8 be taper tube wall, 9 be the mouth of pipe, 10 be its bottom.
Embodiment
With reference to embodiment and embodiment, the present invention is further detailed explanation.
As shown in Figure 1, 2, 3, a kind of PCR pipe, its structure includes lid 1 and body 4, and the top of its middle tube body 4 is provided with pipe Mouth 9 and bottom lock, lid 1 are plugged in the mouth of pipe 9, and lid 1 is the hollow tubular structure of top seal and bottom opening, lid 1 top sets concave plane mirror 2, the top surface of concave plane mirror 2 be less than in the top surface of lid 1, the circumference exterior surface of the bottom of lid 1 set to The arc steps 3 of outer lug, the vertical section of arc steps 3 is arch, and the lower section fit circular arc step 3 of the 4 inner side mouth of pipe of body 9 sets recessed Groove 5, arc steps 3 are interference fitted with groove 5, and the vertical section of the mouth of pipe 9 is isosceles trapezoid, and the first half of body 4 is cylindrical tube Body, lower half is conical tube 6, and conical tube 6 is surrounded by taper tube wall 8, and the bottom of conical tube 6 is its bottom 10, pipe The lower surface of body bottom 10 is body bottom surface 7, and body bottom surface 7 is plane.
The external diameter of the cylindrical tube of described body 4 is that 8.0mm, thickness are 0.5mm, the bottom external diameter of conical tube 6 Be 18 ° for 3.0mm, angle of inclination, thickness be 0.3mm.
A diameter of 4.0mm of described concave plane mirror 2, thickness is 0.8mm, the top surface of concave plane mirror 2 and the top surface of lid 1 away from From for 0.5mm.
A diameter of 5.1mm of the described peak of arc steps 3, the top of groove 5 is from being 2.8mm with a distance from the mouth of pipe.
A diameter of 6.5mm of the described mouth of pipe 9, highly be 1.5mm.
It is hemi-spherical cavities in described its bottom 10, a diameter of 2.6mm, the convenient reagent sample in body is inhaled Dry, reagent sample is difficult to stick to spherical surface.
Described lid 1 is formed in one with body 4 and material is identical.
According to above structure, during closure, lid can be imported into the groove in body by mouth of pipe inclined-plane, made in lid Step can interference in groove, reach good sealing effectiveness;The angle and wall thickness of body taper surface are reasonable in design, improve with The uniformity that PCR instrument adapter coordinates;Body easily can uprightly be put on the table by the planar design of its bottom Put.
Above-described is only the preferred embodiment of the present invention, it is noted that for one of ordinary skill in the art For, without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to the present invention Protection domain.

Claims (7)

1. a kind of PCR pipe, its feature includes being provided with the mouth of pipe (9) and bottom at the top of lid (1) and body (4), its middle tube body (4) Closing, lid (1) is plugged in the mouth of pipe (9), and lid (1) is the hollow tubular structure of top seal and bottom opening, lid (1) Top sets concave plane mirror (2), and the top surface of concave plane mirror (2) is less than the top surface of lid (1), and the circumference of lid (1) bottom is to appearance The arc steps (3) of outwardly convex are set on face, the vertical section of arc steps (3) is the mouth of pipe (9) lower section on the inside of arch, body (4) Fit circular arc step (3) sets groove (5), and arc steps (3) are interference fitted with groove (5), and the vertical section of the mouth of pipe (9) is isosceles Trapezoidal, body (4) first half is cylindrical tube, and lower half is conical tube (6), and conical tube (6) is enclosed by taper tube wall (8) Into the bottom of conical tube (6) is its bottom (10), and the lower surface of its bottom (10) is body bottom surface (7), body bottom surface (7) it is plane.
2. a kind of PCR pipe as claimed in claim 1, it is characterized in that the external diameter of the cylindrical tube of described body (4) is 8.0mm, thickness are 0.5mm, the bottom external diameter of conical tube (6) be 3.0mm, angle of inclination be 18 °, thickness be 0.3mm.
3. a kind of PCR pipe as claimed in claim 1, it is characterized in that a diameter of 4.0mm of described concave plane mirror (2), thickness For 0.8mm, the distance of concave plane mirror (2) top surface and lid (1) top surface is 0.5mm.
4. a kind of PCR pipe as claimed in claim 1, it is characterized in that described arc steps (3) peak is a diameter of 5.1mm, from being 2.8mm with a distance from the mouth of pipe at the top of groove (5).
5. a kind of PCR pipe as claimed in claim 1, it is characterized in that a diameter of 6.5mm of the described mouth of pipe (9), being highly 1.5mm。
6. a kind of PCR pipe as claimed in claim 1, it is characterized in that being hemi-spherical cavities in described its bottom (10), directly Footpath is 2.6mm.
7. a kind of PCR pipe as claimed in claim 1, it is characterized in that described lid (1) and body (4) is formed in one and material Matter is identical.
CN201710411444.8A 2017-06-05 2017-06-05 A kind of PCR pipe Pending CN106967592A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710411444.8A CN106967592A (en) 2017-06-05 2017-06-05 A kind of PCR pipe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710411444.8A CN106967592A (en) 2017-06-05 2017-06-05 A kind of PCR pipe

Publications (1)

Publication Number Publication Date
CN106967592A true CN106967592A (en) 2017-07-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710411444.8A Pending CN106967592A (en) 2017-06-05 2017-06-05 A kind of PCR pipe

Country Status (1)

Country Link
CN (1) CN106967592A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109735437A (en) * 2019-01-28 2019-05-10 长春长光辰英生物科学仪器有限公司 It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102921485A (en) * 2012-10-30 2013-02-13 无锡耐思生物科技有限公司 Centrifuge tube structure
US20140271408A1 (en) * 2013-03-13 2014-09-18 Dale Singh Taunk Microtube cap
CN205077049U (en) * 2015-08-27 2016-03-09 东莞市积健生物科技有限公司 Seal structure of PCR tubes , PCR tubes strip and PCR tubes board
CN206927884U (en) * 2017-06-05 2018-01-26 无锡国盛生物工程有限公司 A kind of PCR pipe

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102921485A (en) * 2012-10-30 2013-02-13 无锡耐思生物科技有限公司 Centrifuge tube structure
US20140271408A1 (en) * 2013-03-13 2014-09-18 Dale Singh Taunk Microtube cap
CN205077049U (en) * 2015-08-27 2016-03-09 东莞市积健生物科技有限公司 Seal structure of PCR tubes , PCR tubes strip and PCR tubes board
CN206927884U (en) * 2017-06-05 2018-01-26 无锡国盛生物工程有限公司 A kind of PCR pipe

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109735437A (en) * 2019-01-28 2019-05-10 长春长光辰英生物科学仪器有限公司 It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell

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