CN106962714A - The high gravity fermentation method of lemon polyphenol - Google Patents
The high gravity fermentation method of lemon polyphenol Download PDFInfo
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- CN106962714A CN106962714A CN201710355632.3A CN201710355632A CN106962714A CN 106962714 A CN106962714 A CN 106962714A CN 201710355632 A CN201710355632 A CN 201710355632A CN 106962714 A CN106962714 A CN 106962714A
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- 235000005979 Citrus limon Nutrition 0.000 title claims abstract description 103
- 244000248349 Citrus limon Species 0.000 title claims abstract description 98
- 238000000855 fermentation Methods 0.000 title claims abstract description 62
- 230000004151 fermentation Effects 0.000 title claims abstract description 56
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 38
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000005484 gravity Effects 0.000 title claims abstract description 18
- 241000195493 Cryptophyta Species 0.000 claims abstract description 57
- 241000196324 Embryophyta Species 0.000 claims abstract description 46
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 45
- 230000000694 effects Effects 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims description 30
- 241000894006 Bacteria Species 0.000 claims description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 7
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 6
- 239000000287 crude extract Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 244000131522 Citrus pyriformis Species 0.000 claims description 5
- 229920002678 cellulose Polymers 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 5
- 238000007792 addition Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 241000208688 Eucommia Species 0.000 claims description 3
- 241000208686 Eucommiaceae Species 0.000 claims description 3
- 241000233866 Fungi Species 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000012074 organic phase Substances 0.000 claims description 3
- 239000012071 phase Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 241000191996 Pediococcus pentosaceus Species 0.000 claims 1
- 239000000835 fiber Substances 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
- 230000035622 drinking Effects 0.000 abstract description 6
- 230000036541 health Effects 0.000 abstract description 4
- 235000019463 artificial additive Nutrition 0.000 abstract description 3
- 239000010985 leather Substances 0.000 abstract description 3
- 230000000116 mitigating effect Effects 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 7
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 3
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
- 229940090949 docosahexaenoic acid Drugs 0.000 description 2
- 150000002972 pentoses Chemical class 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000009088 Citrus pyriformis Nutrition 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of high gravity fermentation method of lemon polyphenol, the lemon fermented method for the lifting lemon polyphenol content that the present invention is provided, by using the endophyte of plant and algae tunning, the lemon peel juice is fermented, to lift the content of lemon polyphenol in the lemon peel juice, the effect for facilitating drinking person health is reached.The lemon fermented method for the lifting lemon polyphenol content that the present invention is provided, fermented by using the belt leather lemon of whole, and without any sugared part of addition, lemon peel juice sugariness can reach 9 11 ° of Brix after fermentation, and without helping user's entrance by extra artificial additive, reach the effect for mitigating drinking person body burden.
Description
Technical field
The present invention relates to a kind of fermentation process, the high concentration hair of particularly a kind of lemon polyphenol for lifting lemon polyphenol content
Fermenting process.
Background technology
Lemon (Lemon), scientific name Citruslimon (L.) Brum.f, lemon is rich in abundant Vitamin C, polyphenol, lemon
The nutriments such as acid, such as polyphenol, are the derivative of aromatic group, it is considered to be good antioxidant, have to many diseases
Good curative effect.Therefore intake lemon polyphenol has turned into one of regimen that modern keeps fit.
In general, after the common people can often remove the peel lemon, the juice (that is, lemon juice) for squeezing Limon pulp is drunk, still,
Lemon polyphenol content contained by Limon pulp is not high, and squeezes the acidity height of the lemon juice of gained, often needs extra addition sugared
Or honey is to adjust mouthfeel.In view of this, it is necessary to which a kind of lemon fermented method for lifting lemon polyphenol content, lemon is rich in obtain
The lemon peel juice of lemon polyphenol.
The content of the invention
The present invention provides a kind of high gravity fermentation method of lemon polyphenol, to obtain the lemon peel juice of high lemon polyphenol content
Zymotic fluid, and obtain the lemon peel juice zymotic fluid of easy entrance.
The technical scheme is that：The high gravity fermentation method of lemon polyphenol, including：A lemon peel juice is provided, to incite somebody to action
The whole lemon juicing containing skin is obtained；One endophyte of plant is provided；One algae tunning is provided；Mix the lemon peel juice,
The endophyte of plant and the algae tunning, to form a mixture, make the mushroom material ultimate density in the mixture be
2.8×105CFU/ml-3.6×106CFU/ml；With the endophyte of plant and the algae tunning co-fermentation lemon peel juice,
Wherein fermentation temperature is 17 DEG C -37 DEG C, and fermentation time is 30-60 days, to obtain a lemon peel juice zymotic fluid.
Further, fermentation temperature is 17 DEG C -28 DEG C.
Further, fermentation time is 40-50 days.
Further, fermentation temperature is 26 DEG C.
Further, fermentation time is 45 days.
Further, the mixture is is made up of the lemon peel juice, the bacterium solution of endophyte of plant and algae tunning.
Further, in the mixture, the bacterium number of the endophyte of plant and algae tunning is identical.
Further, the extracting method of the endophyte of plant is：Using tissue block method, the fresh bark of eucommia i.e. Eucommiaceae is taken to plant
The bark of thing, after surface sterilization processing, is seeded to PDA（Separate endogenetic fungus）With Gause I culture medium（Raw unwrapping wire in separation
Bacterium）Upper culture 7-10d, then separates single bacterium colony；Picking inoculated by hypha block is in equipped with 100mL fermentation mediums from activated inclined plane
Conical flask in, put 28 DEG C, 200r/min shaking table shaken cultivations 7d；Fermentation culture medium removes thalline, and filtrate uses isometric acetic acid
Ethyl ester is extracted 3 times, merges organic phase, and 55 DEG C are concentrated under reduced pressure and are evaporated, as bacterium solution crude extract；The thalline for centrifuging and being filtrated to get,
With appropriate 95% ethanol condensing reflux 3 times, merge ethanol phase, be concentrated under reduced pressure and be evaporated, as thalline crude extract；Put 4 DEG C of refrigerator preservations
It is standby.
Further, the algae tunning is splits kettle algae tunning, and its preparation method is：Over cleaning of learning from else's experience is split
Kettle algae raw material, sets enzymatic hydrolysis condition as solid-liquid ratio 1: 10（m/V）, cellulase CellulaseACCF-4740 additions 2%, temperature
55 DEG C of degree, pH4.5, reaction time 1.3h, are digested, after cellulose hydrolyzation terminates, and boiling water bath goes out enzyme 15min, 9000r/
Min is centrifuged, and is collected supernatant and is obtained splitting kettle algae cellulose hydrolyzation liquid；Kettle algae enzymolysis liquid is split to go out after enzyme activity, regulation pH value to
6.2-6.6, high pressure steam sterilization（121 DEG C, 20min）, then it is inoculated with the pentose piece ball of the MRS culture mediums activation of volume fraction 1%
Bacterium solution stands, fermented to kettle algae enzymolysis liquid, 37 DEG C of constant incubators are split；After fermentation ends, 9000r/min centrifugations are collected
Supernatant obtains splitting kettle algae tunning.
The focus that natural fermented material is always the research of scientists from all over the world, domestic and international different researchers pair are found from plant
The research prompting of numerous plants, but the more difficult simulation of plant growth environment, growth cycle length and Activities of Some Plants are all endangered, this
A large amount of productions to natural fermented active material bring very big difficulty.Endophyte of plant（Endophyte）It is to be present in healthy plant
In thing tissue and organ, and infected host plant does not show substantially to infect a quasi-microorganism of symptom.Research to it
It was found that and its correlation Quality Research show, the presence of endophyte, and many endophytes are all accompanied by almost all of plant
The secondary metabolite with the same or analogous tool bioactivity of host plant can be produced under long-term natural selection, this is
It was found that the extraction of New function activated product and active material provides important development and utilization approach.
Marine microalgae as the original and highly important living marine resources of a class, rich in polyunsaturated fatty acid, polysaccharide,
The various bioactivators such as polypeptide, now mainly for the preparation of bioenergy and aquaculture feed, on the work for fermentation
Property material prepare report it is more rare.It is the microalgae that a class is rich in polyunsaturated fatty acid to split kettle algae, is often used to production high
Purity docosahexaenoic acid（Docosahexaenoic acid, DHA）Health products, infant's dairy products additive etc..Split
Algae-residue its protein content produced by after the extracted polyunsaturated fatty acid of kettle algae may be up to more than 40%, however at present these
Algae-residue is treated as feed or soil fertility quality mostly, and protein resource is not fully developed and utilized.The production of kettle algae fermentation protein will be split
Thing is applied to lemon polyphenol fermenting and producing, and to improve lemon polyphenol product production quantifier elimination, there is not been reported both at home and abroad.
The lemon fermented method for the lifting lemon polyphenol content that the present invention is provided, by using the endophyte of plant and algae
Tunning, the lemon peel juice is fermented, and to lift the content of lemon polyphenol in the lemon peel juice, is reached and is facilitated drinking person
Effect of health.The lemon fermented method for the lifting lemon polyphenol content that the present invention is provided, by using the belt leather lemon of whole
Fermented, and without any sugared part is added, lemon peel juice sugariness can reach 9-11 ° of Brix after fermentation, and without by extra
Artificial additive helps user's entrance, reaches the effect for mitigating drinking person body burden.
Embodiment
Technical scheme is clearly and completely described below in conjunction with the embodiment of the present invention, it is clear that retouched
The embodiment stated is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, sheet
The every other embodiment that field those of ordinary skill is obtained under the premise of creative work is not made, belongs to the present invention
The scope of protection.
Embodiment 1
The high gravity fermentation method of lemon polyphenol, including：A lemon peel juice is provided, by the whole lemon juicing containing skin is obtained
；One endophyte of plant is provided；One algae tunning is provided；Mix the lemon peel juice, the endophyte of plant and algae hair
Ferment product, to form a mixture, it is 2.8 × 10 to make the mushroom material ultimate density in the mixture5CFU/ml；With the plant
Endophyte and the algae tunning co-fermentation lemon peel juice, wherein fermentation temperature are 37 DEG C, and fermentation time is 60 days, to obtain
Obtain a lemon peel juice zymotic fluid.
Further, the mixture is is made up of the lemon peel juice, the bacterium solution of endophyte of plant and algae tunning.
Further, in the mixture, the bacterium number of the endophyte of plant and algae tunning is identical.
Further, the extracting method of the endophyte of plant is：Using tissue block method, the fresh bark of eucommia i.e. Eucommiaceae is taken to plant
The bark of thing, after surface sterilization processing, is seeded to PDA（Separate endogenetic fungus）With Gause I culture medium（Raw unwrapping wire in separation
Bacterium）Upper culture 7-10d, then separates single bacterium colony；Picking inoculated by hypha block is in equipped with 100mL fermentation mediums from activated inclined plane
Conical flask in, put 28 DEG C, 200r/min shaking table shaken cultivations 7d；Fermentation culture medium removes thalline, and filtrate uses isometric acetic acid
Ethyl ester is extracted 3 times, merges organic phase, and 55 DEG C are concentrated under reduced pressure and are evaporated, as bacterium solution crude extract；The thalline for centrifuging and being filtrated to get,
With appropriate 95% ethanol condensing reflux 3 times, merge ethanol phase, be concentrated under reduced pressure and be evaporated, as thalline crude extract；Put 4 DEG C of refrigerator preservations
It is standby.
Further, the algae tunning is splits kettle algae tunning, and its preparation method is：Over cleaning of learning from else's experience is split
Kettle algae raw material, sets enzymatic hydrolysis condition as solid-liquid ratio 1: 10（m/V）, cellulase CellulaseACCF-4740 additions 2%, temperature
55 DEG C of degree, pH4.5, reaction time 1.3h, are digested, after cellulose hydrolyzation terminates, and boiling water bath goes out enzyme 15min, 9000r/
Min is centrifuged, and is collected supernatant and is obtained splitting kettle algae cellulose hydrolyzation liquid；Kettle algae enzymolysis liquid is split to go out after enzyme activity, regulation pH value to
6.2-6.6, high pressure steam sterilization（121 DEG C, 20min）, then it is inoculated with the pentose piece ball of the MRS culture mediums activation of volume fraction 1%
Bacterium solution stands, fermented to kettle algae enzymolysis liquid, 37 DEG C of constant incubators are split；After fermentation ends, 9000r/min centrifugations are collected
Supernatant obtains splitting kettle algae tunning.
The lemon fermented method for the lifting lemon polyphenol content that the present invention is provided, by using the endophyte of plant and algae
Tunning, the lemon peel juice is fermented, and to lift the content of lemon polyphenol in the lemon peel juice, is reached and is facilitated drinking person
Effect of health.The lemon fermented method for the lifting lemon polyphenol content that the present invention is provided, by using the belt leather lemon of whole
Fermented, and without any sugared part is added, lemon peel juice sugariness can reach 9-11 ° of Brix after fermentation, and without by extra
Artificial additive helps user's entrance, reaches the effect for mitigating drinking person body burden.
Embodiment 2
The high gravity fermentation method of lemon polyphenol, including：A lemon peel juice is provided, by the whole lemon juicing containing skin is obtained
；One endophyte of plant is provided；One algae tunning is provided；Mix the lemon peel juice, the endophyte of plant and algae hair
Ferment product, to form a mixture, it is 3.6 × 10 to make the mushroom material ultimate density in the mixture6CFU/ml；With the plant
Endophyte and the algae tunning co-fermentation lemon peel juice, wherein fermentation temperature are 17 DEG C DEG C, and fermentation time is 30 days, with
Obtain a lemon peel juice zymotic fluid.
Further, the mixture is is made up of the lemon peel juice, the bacterium solution of endophyte of plant and algae tunning.
Further, in the mixture, the bacterium number of the endophyte of plant and algae tunning is identical.
Embodiment 3
The high gravity fermentation method of lemon polyphenol, including：A lemon peel juice is provided, by the whole lemon juicing containing skin is obtained
；One endophyte of plant is provided；One algae tunning is provided；Mix the lemon peel juice, the endophyte of plant and algae hair
Ferment product, to form a mixture, it is 8.6 × 10 to make the mushroom material ultimate density in the mixture5CFU/ml；With the plant
Endophyte and the algae tunning co-fermentation lemon peel juice, wherein fermentation temperature are 28 DEG C, and fermentation time is 50 days, to obtain
Obtain a lemon peel juice zymotic fluid.
Further, fermentation temperature is 17 DEG C -28 DEG C.
Further, fermentation time is 40-50 days.
Further, fermentation temperature is 26 DEG C.
Further, fermentation time is 45 days.
Further, the mixture is is made up of the lemon peel juice, the bacterium solution of endophyte of plant and algae tunning.
Further, in the mixture, the bacterium number of the endophyte of plant and algae tunning is identical.
Embodiment 4
The high gravity fermentation method of lemon polyphenol, including：A lemon peel juice is provided, by the whole lemon juicing containing skin is obtained
；One endophyte of plant is provided；One algae tunning is provided；Mix the lemon peel juice, the endophyte of plant and algae hair
Ferment product, to form a mixture, it is 1.5 × 10 to make the mushroom material ultimate density in the mixture6CFU/ml；With the plant
Endophyte and the algae tunning co-fermentation lemon peel juice, wherein fermentation temperature are 26 DEG C, and fermentation time is 45 days, to obtain
Obtain a lemon peel juice zymotic fluid.
Further, the mixture is is made up of the lemon peel juice, the bacterium solution of endophyte of plant and algae tunning.
Further, in the mixture, the bacterium number of the endophyte of plant and algae tunning is identical.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.The ins and outs not being described in detail in the present invention, can pass through any in this area
Prior art is realized.Particularly, all technical characterstics not being described in detail can be realized by any prior art in the present invention.
Claims (9)
1. the high gravity fermentation method of lemon polyphenol, it is characterised in that：Including：A lemon peel juice is provided, for by whole containing skin
Lemon juicing is obtained；One endophyte of plant is provided；One algae tunning is provided；Mix the lemon peel juice, the plant endogenesis
Bacterium and the algae tunning, to form a mixture, make mushroom material ultimate density in the mixture for 2.8 ×
105CFU/ml-3.6×106CFU/ml；With the endophyte of plant and the algae tunning co-fermentation lemon peel juice, wherein sending out
Ferment temperature is 17 DEG C -37 DEG C, and fermentation time is 30-60 days, to obtain a lemon peel juice zymotic fluid.
2. the high gravity fermentation method of lemon polyphenol as claimed in claim 1, it is characterised in that：Fermentation temperature is 17 DEG C -28
℃。
3. the high gravity fermentation method of lemon polyphenol as claimed in claim 1, it is characterised in that：Fermentation time is 40-50 days.
4. the high gravity fermentation method of lemon polyphenol as claimed in claim 2, it is characterised in that：Fermentation temperature is 26 DEG C.
5. the high gravity fermentation method of lemon polyphenol as claimed in claim 3, it is characterised in that：Fermentation time is 45 days.
6. the high gravity fermentation method of lemon polyphenol as claimed in claim 1, it is characterised in that：The mixture is by the lemon
Skin juice, the bacterium solution of endophyte of plant and algae tunning are constituted.
7. the high gravity fermentation method of the lemon polyphenol as described in claim 1, it is characterised in that：In the mixture, the plant
The bacterium number of thing endophyte and algae tunning is identical.
8. the high gravity fermentation method of the lemon polyphenol as described in claim 1, it is characterised in that：The endophyte of plant
Extracting method is：Using tissue block method, the fresh bark of eucommia i.e. bark of Eucommiaceae plant is taken, after surface sterilization processing, PDA is seeded to
（Separate endogenetic fungus）With Gause I culture medium（Separate endogeny rayungus）Upper culture 7-10d, then separates single bacterium colony；From work
Change picking inoculated by hypha block in inclined-plane and in the conical flask equipped with 100mL fermentation mediums, put 28 DEG C, the vibration of 200r/min shaking tables
Cultivate 7d；Fermentation culture medium removes thalline, and filtrate is extracted 3 times with isometric ethyl acetate, merges organic phase, 55 DEG C are concentrated under reduced pressure
It is evaporated, as bacterium solution crude extract；The thalline for centrifuging and being filtrated to get, with appropriate 95% ethanol condensing reflux 3 times, merges ethanol phase,
It is concentrated under reduced pressure and is evaporated, as thalline crude extract；4 DEG C of refrigerators are put to save backup.
9. the high gravity fermentation method of the lemon polyphenol as described in claim 1, it is characterised in that：The algae tunning
To split kettle algae tunning, its preparation method is：Over cleaning of learning from else's experience splits kettle algae raw material, sets enzymatic hydrolysis condition as solid-liquid ratio 1: 10
（m/V）, cellulase CellulaseACCF-4740 additions 2%, 55 DEG C of temperature, pH4.5, reaction time 1.3h, carry out enzyme
Solution, after cellulose hydrolyzation terminates, boiling water bath goes out enzyme 15min, and 9000r/min centrifugations collect supernatant and obtain splitting kettle algae fiber
Plain enzyme hydrolyzate；Split kettle algae enzymolysis liquid to go out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20min）,
Then the Pediococcus pentosaceus liquid of the MRS culture mediums activation of inoculation volume fraction 1% is to splitting kettle algae enzymolysis liquid, 37 DEG C of constant incubators
Stand, fermented；After fermentation ends, 9000r/min centrifugations collect supernatant and obtain splitting kettle algae tunning.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710355632.3A CN106962714A (en) | 2017-05-19 | 2017-05-19 | The high gravity fermentation method of lemon polyphenol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710355632.3A CN106962714A (en) | 2017-05-19 | 2017-05-19 | The high gravity fermentation method of lemon polyphenol |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103844219A (en) * | 2014-02-21 | 2014-06-11 | 东莞健茂生物科技有限公司 | Fermentation method for improving polyphenol content of lemon |
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2017
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| CN103844219A (en) * | 2014-02-21 | 2014-06-11 | 东莞健茂生物科技有限公司 | Fermentation method for improving polyphenol content of lemon |
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