CN106959352A - A kind of method acted on using the luxuriant behavior evaluation medicament of volume rice leaf roller - Google Patents
A kind of method acted on using the luxuriant behavior evaluation medicament of volume rice leaf roller Download PDFInfo
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Abstract
The present invention provides a kind of method acted on using the luxuriant behavior evaluation medicament of volume rice leaf roller, and then methods described takes survival larva to carry out the luxuriant behavior observation of volume, record 8 indexs using rice leaf roller larvae is raised after low dosage medicament dipping blade：Silk group number after luxuriant length, 24h is rolled up after selection time, first secondary volume luxuriant time, the luxuriant silk number of lines of initial volume, average every group of silk thread number, silk group number, the luxuriant length of initial volume, 24h：And carry out Statistical Comparison with the control group without chemicals treatment, if without significant difference between each index, then think that medicament does not make significant difference to survival larva, if each index has significant difference, differentiate that medicament has a negative impact or Beneficial Effect to survival larva according to criterion.The inventive method, which will roll up luxuriant behavior, is used for supplementary evaluation medicament to the action effect of rice leaf roller, action effect of the medicament to rice leaf roller especially under low dosage level.The supplement that can be evaluated as medicament rice leaf roller action effect.
Description
Technical field
Rice leaf roller Cnaphalocrocis medinalis effects are commented the present invention relates to medicament in Rice Production
Valency method, belongs to biological technical field.
Background technology
Rice leaf roller (Cnaphalocrocis medinalis) is the important pests in Rice Production, takes food water of causing harm
Rice blade is simultaneously longitudinally formed leaf roll, influences leaf photosynthesis, causes the paddy rice underproduction.Rice leaf roller larvae is first in paddy rice blade tip
Harm, rear toward harm in the middle part of rice leaf, spinning takes food the vertical volume of blade.The luxuriant behavior of the volume of rice leaf roller larvae and its
Take food harm closely related.Rice leaf roller larvae spins since blade one end, bonds to the blade other end 1 silk thread of formation,
A plurality of silk thread one group of silk thread of formation that same position connection is told, then starts change place continuation spinning and forms multigroup silk thread, formed
Initial volume bud, rice leaf is perched or takes food in volume bud.
At present, effect of the medicament to rice leaf roller is mainly evaluated with the death rate, but after chemicals treatment living individuals by
In pharmacy effect, it is understood that there may be the difference in behavior, these differences also determine its harmfulness to plant.After pharmacy effect, if
Quick knot bud, then be conducive to larva to hide, in order to avoid natural enemy is preyed on, it is favourable to increase the exposed time if the luxuriant behavior slowly of volume is formed
Taken food in natural enemy.Situation of the insect through non-lethal dose chemicals treatment is evaluated to be conducive to accurately reflecting effect shape of the medicament to insect
Condition.In order to solve this problem, we have invented the method for evaluating influence of the medicament to rice leaf roller to roll up luxuriant behavior.
The content of the invention
The present invention provides a kind of Behavioral assessment method that evaluation medicament influences on insect, is to study medicament to cnaphalocrocis
The researcher of snout moth's larva effect provides a kind of behavior evaluation method.Main utilize of the invention rolls up the non-lethal agent of luxuriant behavior evaluation low concentration
The medicament of amount is acted on rice leaf roller, and survival insect is by being influenceed after primary evaluation chemicals treatment.
The technical solution adopted by the present invention is：
A kind of method acted on using the luxuriant behavior evaluation medicament of volume rice leaf roller, the described method comprises the following steps：
(1) take the tillering stage rice leaf without pesticide-treated to be cut into segment, 10~60s is impregnated in medicament to be evaluated
(preferably 30s), taking-up is dried, and 2 age rice leaf roller larvaes are raised using the blade after immersion, is raised 1~5 day (preferably 3
My god) after will survival larva take out move to no medicament fed on leaves, to any age in 3-5 ages be used for roll up luxuriant behavior observation；
(2) luxuriant behavior observation is rolled up：Clip tillering stage rice leaf 1, takes to be seen age of the step (1) through chemicals treatment
Rice leaf roller larvae 1, be connected on rice leaf, start simultaneously at timing, following 8 for observing and recording chemicals treatment group
Index：(1) selection time：From worm is connect to the time for telling first filament；(2) first secondary volume luxuriant time：From tell first filament to
The time that initial volume bud is completed；(3) the luxuriant silk number of lines of initial volume：The silk thread sum told in initial volume bud building process；(4) it is average
Every group of silk thread number：The bar number of silk thread in average each group of silk thread；(5) silk group number：The silk thread told in initial volume bud building process
Group number；(6) the luxuriant length of initial volume：Initial volume bud builds the length of volume bud when completing；(7) luxuriant length is rolled up after 24h：Since being connect worm
The length of bud is rolled up after 24h；(8) silk group number after 24h：Since the silk group number that bud is rolled up after 24h connecing worm；Repeat 10-20 times, system
Count the average and standard error of These parameters；
(3) the same instar larvae without chemicals treatment is taken, as a control group, is grasped according to the same method of step (2)
Make, 8 indexs of observed and recorded, repeat 10-20 times, count the average and standard error of These parameters；
(4) chemicals treatment group and the data of control group are subjected to Statistical Comparison analysis, if without significant difference between each index,
Then think not making significant difference to survival larva after chemicals treatment, i.e., medicament is smaller on survival larva subsequent affect or without influence；If
Be present significant difference in each index, then differentiated according to following standard：The first luxuriant time increase of secondary volume is designated as+1, when first secondary volume is luxuriant
Between reduce and be designated as -1, selection time increase is designated as+1, and selection time, which is reduced, is designated as -1, initial volume bud silk number of lines reduce be designated as+
1, initial volume bud silk number of lines increase is designated as -1, and average every group of silk thread number, which is reduced, is designated as+1, and average every group of silk thread number increase be designated as -
1, silk group number, which is reduced, is designated as+1, and silk group number increase is designated as the luxuriant length reduction of -1, initial volume and is designated as+1, and initial volume bud length increases
Plus be designated as rolling up luxuriant length after -1,24h and reduce to be designated as rolling up luxuriant length increase after+1,24h and be designated as silk group number after -1,24h and reduce note
- 1 is designated as silk group number increase after+1,24h；0 is designated as without significant difference；After the fraction that 8 indexs are recorded according to above-mentioned standard
Addition obtains total score, total score is more than 0, and has two or more+1 point, then it is assumed that medicament has a negative impact to survival larva,
Total score is less than 0 and there is two or more -1 point of situation, then it is assumed that medicament produces Beneficial Effect to survival larva.
In general, rolling up luxuriant time, selection time shortening, the luxuriant length of volume, the luxuriant silk group number of volume etc. reduces then explanation increase
It is time of larva exposure, and volume bud is smaller, is unfavorable for hiding, counter to be conducive to natural enemy to take food, that is, think that medicament can continue pair
Survival larva has a negative impact.Said if volume luxuriant time, selection time shorten, roll up the increases such as the luxuriant silk group number of luxuriant length, volume
Bright chemicals treatment does not kill larva not only, produces Beneficial Effect to survival larva on the contrary
In the step (1), the blade after immersion is raised into 2 age rice leaf roller larvaes, preferred method of operation is as follows：
Blade after immersion is laid in culture dish, moisturizing is wrapped up in two ends with wet cotton balls, takes the access culture of 2 age rice leaf roller larvaes
Raised in ware.
In the step (2), tillering stage rice leaf preferably last to third from the bottom fully expanded leaves.
In the present invention, medicament to be evaluated is the medicament of low concentration non-lethal dose, and general concentration does not reach the lethal concentration of 50
Or dosage.The lethal concentration of 50 of different agents is different, and medicament to be evaluated mainly investigates the behavior for the insect that survived after chemicals treatment, because
The concentration of this medicament to be evaluated generally selects the concentration (LC between lethal 1~50%1-LC50)。
The luxuriant behavior of volume can be used to evaluate in medicament insecticidal action effect by the inventive method.
The beneficial effects of the present invention are：
(1) complementarity：Conventional method is mainly judged with the lethal concentration of 50 or dosage of medicament to the vertical volume of target organismses rice
The action effect of leaf snout moth's larva, and under normal circumstances, the insect that medicament is not killed is not considered into then, the insect not killed may be by
In the follow-up effect of medicament, the reduction of causing harm property or raising, behavior would generally react the state of an individual.The present invention is by seeing
Examine after chemicals treatment the luxuriant behavioural characteristic of rice leaf roller volume, supplementary evaluation medicament is to the action effect of rice leaf roller, especially
Action effect of the medicament to rice leaf roller under low dosage level.Rice leaf roller action effect can be evaluated as medicament
Supplement.(2) it is easy, easy to operate：The inventive method is simple and convenient, easy operation, it is only necessary to prepare the simple material such as paddy rice, timer
Material and instrument.
Embodiment
Technical scheme is described further with specific embodiment below, but protection scope of the present invention is not
It is limited to this.
Embodiment 1：The luxuriant behavior observation method (control group processing) of rice leaf roller volume
1st, the preparation and plantation of paddy rice
Take TN1 rice paddy seeds, breeding is planted in cement tub, conventional rich water quality management.It is standby to tillering stage.
2nd, prepare before observing
Last to third from the bottom fully expanded leaves 1 of clip tillering stage paddy rice, is wrapped up at section with wet cotton balls,
It is put into dactylethrae.Repeat number 10-20 batches.
3rd, luxuriant behavior observation is rolled up
The rice leaf roller larvae 1 of 5 ages is taken, is connected on rice leaf, while pressing timer starts timing.Observation
And record following index：(1) selection time：From worm is connect to telling first secondary volume luxuriant time time (2) of first filament：From telling first
The time that root filament is completed to initial volume bud.(3) the luxuriant silk number of lines of initial volume：The silk thread told in initial volume bud building process is total
Number.(4) average every group of silk thread number：The bar number of silk thread in average each group of silk thread.(5) silk group number：Initial volume bud building process
In the silk group number told.(6) the luxuriant length of initial volume：Initial volume bud builds the length of volume bud when completing.(7) luxuriant length is rolled up after 24h：
Since the length that bud is rolled up after 24h connecing worm.(8) silk group number after 24h：Since the silk group number that bud is rolled up after 24h connecing worm.Repeat
Number 10-20 times.
4th, the statistics of luxuriant behavioral indicator is rolled up
Utilize SPSS software statistics average and standard error.
Embodiment 2：Rice leaf roller after the luxuriant behavior observation method evaluating chemical chemicals treatment of volume provided using the present invention
Effect (chemicals treatment group)
Rice leaf roller second instar larvae is handled using 0.007mg/L multiple killing teichomycin, medicament pair is fully understanded in order to understand
The action effect of rice leaf roller, we make an appraisal in the luxuriant behavior of volume by observing rice leaf roller larvae after chemicals treatment.
1st, the preparation and plantation of paddy rice
Take TN1 rice paddy seeds, breeding is planted in cement tub, conventional rich water quality management.Used, the plantation to tillering stage standby experiment
Period is without using any agricultural chemicals.
2nd, chemicals treatment rice leaf roller
Take blade to be cut into 5-7cm segments, 30s is impregnated in 0.007mg/L multiple killing teichomycin, taking-up is dried.Blade is laid on
In culture dish, moisturizing is wrapped up in two ends with wet cotton balls.2 age rice leaf roller larvaes are taken to access in culture dish.To survive larva after 3 days
Taking-up moves to no medicament fed on leaves, is used to roll up luxuriant behavior observation to 5 ages.Using larva on without chemicals treatment blade as right
According to group (embodiment 1).
The luxuriant behavior observations of volume 3 prepare
Last to third from the bottom fully expanded leaves 1 of clip tillering stage rice leaf, uses wet cotton balls at section
Parcel, is put into dactylethrae.Repeat 10-20 batches.
The luxuriant behavior observations of volume 4
The rice leaf roller larvae 1 of age to be seen is taken, is connected on rice leaf, while pressing timer starts meter
When.Observe and record following index：(1) selection time：From worm is connect to the time for telling first filament；(2) first secondary volume luxuriant time：
From first filament is told to the initial volume luxuriant time completed.(3) the luxuriant silk number of lines of initial volume：Told in initial volume bud building process
Silk thread sum.(4) average every group of silk thread number：The bar number of filament in each group of silk thread.(5) silk group number：Initial volume bud was built
The silk group number told in journey.(6) the luxuriant length of initial volume：Initial volume bud builds the length of volume bud when completing.(7) bud is rolled up after 24h long
Degree：Since the length that bud is rolled up after 24h connecing worm.(8) silk group number after 24h：Since the silk group number that bud is rolled up after 24h connecing worm.
Repeat number 10-20 batches.
5th, A+E
Correlated results is arranged by observing, specification table 1 is seen.As a result show, rice leaf roller survives after chemicals treatment
Larva selection time extends, and the luxuriant length of volume is shorter, and initial volume bud silk group number and 24h volumes of luxuriant silk group number also tail off compared with control, and 8
5 have significant difference in individual index, according to standards of grading, and the luxuriant time increase of first secondary volume is designated as+1, and the first secondary volume luxuriant time subtracts
- 1 is designated as less, selection time increase is designated as+1, selection time, which is reduced, is designated as -1, and initial volume bud silk number of lines, which is reduced, is designated as+1, just
The luxuriant silk number of lines increase of beginning volume is designated as -1, and average every group of silk thread number, which is reduced, is designated as+1, and averagely every group of silk thread number increase is designated as -1, silk
Line group number, which is reduced, is designated as+1, and silk group number increase is designated as the luxuriant length reduction of -1, initial volume and is designated as+1, initial volume bud length increase note
Reduce to roll up luxuriant length after -1,24h and be designated as rolling up luxuriant length increase after+1,24h and be designated as silk group number after -1,24h and reduce to be designated as+1,
The increase of silk group number is designated as -1 after 24h；0 is designated as without significant difference；It is added after being kept the score according to above-mentioned standard and obtains total score, always
It is divided into+5 points, and there is 5 kinds+1 point, non-- 1 point of presence, therefore, it is determined that 0.007mg/L multiple killing teichomycin is to rice leaf roller larvae
There is adverse effect in the larva that survives.Selection time extends, and the luxuriant length of volume is shorter, initial volume bud silk group number and 24h volumes of luxuriant silk thread
Group number reduces the time for the exposure for adding larva, and the luxuriant parameter of volume shows that volume bud is smaller, is unfavorable for hiding, is conducive to day on the contrary
Enemy takes food.
Influence of the chemicals treatment of table 1 to the luxuriant behavior of volume of rice leaf roller survival larva
In table 1, * represents that control group has significant difference with treatment group.
Claims (10)
1. a kind of method act on using the luxuriant behavior evaluation medicament of volume rice leaf roller, it is characterised in that methods described including with
Lower step：
(1) take the tillering stage rice leaf without pesticide-treated to be cut into segment, 10~60s is impregnated in medicament to be evaluated, take out
Dry, 2 age rice leaf roller larvaes are raised using the blade after immersion, survival larva is taken out after raising 1~5 day and moves to nothing
Medicament fed on leaves, is used to roll up luxuriant behavior observation to any age in 3-5 ages；
(2) luxuriant behavior observation is rolled up：Clip tillering stage rice leaf 1, takes the rice of to be seen age of the step (1) through chemicals treatment
Leaf-roller larva 1, is connected on rice leaf, starts simultaneously at timing, observes and record following 8 fingers of chemicals treatment group
Mark：(1) selection time：From worm is connect to the time for telling first filament；(2) first secondary volume luxuriant time：From first filament is told to first
Begin the volume luxuriant time completed；(3) the luxuriant silk number of lines of initial volume：The silk thread sum told in initial volume bud building process；(4) it is average every
Group silk thread number：The bar number of silk thread in average each group of silk thread；(5) silk group number：The silk group told in initial volume bud building process
Number；(6) the luxuriant length of initial volume：Initial volume bud builds the length of volume bud when completing；(7) luxuriant length is rolled up after 24h：Since being connect worm
The length of bud is rolled up after 24h；(8) silk group number after 24h：Since the silk group number that bud is rolled up after 24h connecing worm；Repeat 10-20 times, system
Count the average and standard error of These parameters；
(3) the same instar larvae without chemicals treatment is taken, as a control group, is operated according to the same method of step (2), is seen
8 indexs of record are examined, are repeated 10-20 times, the average and standard error of These parameters is counted；
(4) chemicals treatment group and the data of control group are subjected to Statistical Comparison analysis, if recognizing between each index without significant difference
Not made significant difference after chemicals treatment to survival larva；If each index has significant difference, sentenced according to following standard
Not：The first luxuriant time increase of secondary volume is designated as+1, and the first secondary volume luxuriant time, which is reduced, is designated as -1, and selection time increase is designated as+1, selection time
Reduction is designated as -1, and initial volume bud silk number of lines, which is reduced, is designated as+1, and initial volume bud silk number of lines increase is designated as -1, average every group of silk thread
Number reductions are designated as+1, and average every group of silk thread number increase is designated as -1, and silk group number, which is reduced, is designated as+1, silk group number increase is designated as -1,
The luxuriant length of initial volume, which is reduced, is designated as+1, and initial volume bud length increase is designated as the luxuriant length reduction of volume after -1,24h and is designated as rolling up after+1,24h
Luxuriant length increase is designated as after -1,24h silk group number and reduced being designated as silk group number increase after+1,24h and being designated as -1；Without significant difference note
For 0；It is added after the fraction that 8 indexs are recorded according to above-mentioned standard and obtains total score, total score is more than 0, and has two or more+1 point
, then it is assumed that medicament has a negative impact to survival larva, and total score is less than 0 and there is two or more -1 point of situation, then it is assumed that medicine
Agent produces Beneficial Effect to survival larva.
2. the method as described in claim 1, it is characterised in that in the step (1), dip time is 30s.
3. the method as described in claim 1, it is characterised in that in the step (1), 2 age rice are raised by the blade after immersion
The operating method of leaf-roller larva is as follows：Blade after immersion is laid in culture dish, moisturizing is wrapped up in two ends with wet cotton balls, takes
Raised in 2 age rice leaf roller larvaes access culture dish.
4. the method as described in claim 1, it is characterised in that in the step (1), raised for 2 ages using the blade after immersion
Rice leaf roller larvae, feeding time is 3 days.
5. the method as described in claim 1, it is characterised in that in the step (2), the tillering stage rice leaf is inverse
First to third from the bottom fully expanded leaves.
6. the method as described in claim 1, it is characterised in that in the step (1), the volume bud of the observation rice leaf roller
The age of behavior was 5 ages.
7. the method as described in claim 1, it is characterised in that the step (1) operates by the following method：Take without at agricultural chemicals
The tillering stage rice leaf of reason is cut into segment, and 30s is impregnated in medicament to be evaluated, and taking-up is dried, and the blade after immersion is laid on
In culture dish, moisturizing is wrapped up in two ends with wet cotton balls, takes 2 age rice leaf roller larvaes to access and is raised in culture dish, raises 3
Survival larva is taken out after it and moves to no medicament fed on leaves, is used to roll up luxuriant behavior observation to 5 ages.
8. the method as described in claim 1, it is characterised in that in the step (1), the concentration of the medicament to be evaluated is low
Concentration pesticidal chemical.
9. the method as described in claim 1, it is characterised in that the concentration of the medicament to be evaluated select lethal 1~50% it
Between concentration.
10. the method as described in claim 1, it is characterised in that in the step (2) or step (3), is united using SPSS softwares
Count average and standard error.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102037879A (en) * | 2009-10-19 | 2011-05-04 | 黄后新 | Method for preventing and controlling cnaphalocrocis medinalis at later stage of rice |
| CN104255355A (en) * | 2014-10-07 | 2015-01-07 | 浙江省农业科学院 | Banker plant system for preventing and curing rice leaf folders and rice stem borers |
| CN104957122A (en) * | 2015-05-25 | 2015-10-07 | 南京农业大学 | Rice leaf roller acquisition and biological assay method |
| CN105432659A (en) * | 2015-11-24 | 2016-03-30 | 贵州省植物保护研究所 | Compound agent for controlling Sogatella furcifera and synergism evaluation method of compound agent |
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2017
- 2017-03-28 CN CN201710194732.2A patent/CN106959352B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102037879A (en) * | 2009-10-19 | 2011-05-04 | 黄后新 | Method for preventing and controlling cnaphalocrocis medinalis at later stage of rice |
| CN104255355A (en) * | 2014-10-07 | 2015-01-07 | 浙江省农业科学院 | Banker plant system for preventing and curing rice leaf folders and rice stem borers |
| CN104957122A (en) * | 2015-05-25 | 2015-10-07 | 南京农业大学 | Rice leaf roller acquisition and biological assay method |
| CN105432659A (en) * | 2015-11-24 | 2016-03-30 | 贵州省植物保护研究所 | Compound agent for controlling Sogatella furcifera and synergism evaluation method of compound agent |
Non-Patent Citations (2)
| Title |
|---|
| M. PUNITHAVALLI等: "Influence of Rice Genotypes on Folding and Spinning Behaviour of Leaffolder (Cnaphalocrocis medinalis) and Its Interaction with Leaf Damage", 《RICE SCIENCE》 * |
| 王彩云等: "不同地理种群稻纵卷叶螟的解毒酶活性及对药剂的敏感性", 《浙江农业学报》 * |
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