CN106947018A - A kind of high-performance and highly controllable hud typed trace sensor and preparation method and purposes - Google Patents

A kind of high-performance and highly controllable hud typed trace sensor and preparation method and purposes Download PDF

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CN106947018A
CN106947018A CN201710136136.9A CN201710136136A CN106947018A CN 106947018 A CN106947018 A CN 106947018A CN 201710136136 A CN201710136136 A CN 201710136136A CN 106947018 A CN106947018 A CN 106947018A
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ethanol
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CN106947018B (en
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李洪吉
姜佳琪
徐叶青
卢凯
刘锡清
李春香
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Jiangsu University
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Abstract

The invention provides a kind of high-performance and highly controllable hud typed trace sensor and preparation method and purposes, preparation process is as follows：The preparation of step 1, amino functional Nano particles of silicon dioxide；The preparation of step 2, functionalization SiO2/Ag nano-complex particles；The preparation of step 3, nucleocapsid SiO2/Ag/MIPs.SERS technologies are combined by the present invention with surface molecule print technology so that the product of preparation has the high sensitivity of SERS Detection Techniques and MIT high selectivity concurrently；Present invention selection molecular imprinted polymer on surface (SMIPs) promotes the selectivity of tradition SERS backing materials, expands the application of SERS detections.

Description

A kind of high-performance and highly controllable hud typed trace sensor and preparation method and Purposes
Technical field
The present invention relates to the preparation and application of a kind of high-performance and highly controllable hud typed trace sensor, belong to new material Technical field.
Background technology
In recent years, organic pollution serious harm global environment, threatens the ecological balance and the health of the mankind.However, mostly Number organic pollution exists only in trace or ultra trace level, time-consuming using traditional detection method, complicated, and is extremely difficult to sensitive Degree demand, carries out accurate detection relative difficulty.Therefore, it is badly in need of developing effective and sensitive detection method.
SERS (SERS) is a kind of effective analysis method, it is possible to achieve tracing detection.When probe point Son SERS stromal surfaces or it is neighbouring when, the Raman signal of probe molecule can significantly be strengthened.In general, SERS strengthens Mechanism has two, respectively physics enhancing and Chemical enhancement.The former is due to that SERS active substrate surface plasmon oscillations are drawn The localized electromagnetic field enhancing risen so that Raman signal is been significantly enhanced.The latter be due to then probe molecule and SERS substrates it Between chemical action so that probe molecule polarizability increase caused by Raman signal strengthen.Under normal circumstances, both strengthen Mechanism is acted on simultaneously causes Raman signal to strengthen, and simply there are different contribution proportions in different systems.
At present, noble metal nano particles (such as gold, silver) are widely used in the preparation of SERS host materials, mainly due to your gold Metal nano-particle has outstanding optics, electrical properties, and has strong characteristic absorption in visibility region, and this is largely It is attributed to its surface plasma body resonant vibration.Wherein, silver-colored use is widest, is primarily due to the SERS signal enhancing of silver the most Substantially, and silver chemical is stable in properties, with broad-spectrum antibiotic property and potential anti-cancer applications.At present, SERS technologies are wide It is general to be applied to detection trace level chemical substance, in such as protein, Medicines, food additives and the identification of a variety of biotic components.So And, the research on SERS at present is mainly concentrated in the lifting of host material pattern or SERS performances, and ignores biography Host material of uniting lacks specific selectivity.Therefore, the selectivity of tradition SERS base materials is lifted, answering for SERS detections is will be enlarged by With.
Recently, molecularly imprinted polymer (MIPs) is excellent by feat of specific recognition, structure effect precordainment and extensive practicality etc. Different characteristic, receives much concern in chromatographic isolation, UF membrane, SPE, drug controlled release, chemical sensitisation, environment measuring.Point Sub- engram technology is to form multiple action site when template molecule (microsphere) is contacted with polymer monomer, passes through polymerization Process produces specific recognition site, after template molecule is removed, is formed in polymer and template molecule steric configuration phase The site hole of matching, such hole will have selection evident characteristics to template molecule and the like.In MIPs materials, Molecular imprinted polymer on surface (SMIPs) binds ability because can preferably solve traditional MIPs shortcoming, such as, with reference to dynamic Mechanical property is not good, too deep, template molecule the removal of avtive spot embedding is not thorough, has gradually attracted increasing science work The favor of author.
In order to improve the selectivity of SERS backing materials, SERS technologies and molecular imprinting technology (MIT) are combined, prepared MIPs-SERS sensors.For example, Kamra et al. is prepared for a novel biology sensor, their binding molecule trace polymerizations Thing and SERS (MIPs-SERS) determine melamine in whole milk.Xiao et al. establishes MIP- Based chemical sensors, are detected by SERS to nicotine.These methods present the Optimality of MIPs-SERS technologies Energy.Therefore, the MIT of highly sensitive SERS Detection Techniques and high selectivity is combined, prepares MIPs-SERS sensors, inspection Surveying organic pollutants has feasibility.
The content of the invention
The sensor main will be synthesized by three-step reaction.First, synthesizing amino functionalized SiO 2 nano-particle.By second Alcohol, water and NH3·H2O is equably mixed, and tetraethyl orthosilicate (TEOS) and mechanical agitation is slowly added dropwise.Again by the second of 3- aminopropyls three TMOS (APTES) is added in solution, continues to stir mixed liquor.After reaction, centrifugation is cleaned with ethanol, is dried；Then, Amino functional Nano particles of silicon dioxide is distributed in the mixed solution of ethanol/water, silver nitrate solution is added, is then added dropwise Polyvinylpyrrolidone (PVP) solution, continues the magnetic agitation in dark surrounds.Then, monoethanolamine is added into mixed system, And raise temperature, continue to stir.Centrifugation, is cleaned with water and ethanol repeatedly, removes unreacted reactant.Final product is in room Temperature is lower to be dried in vacuo；Finally, by rhodamine (R6G), acrylamide (AM) and GDMA (EGDMA) are added Into solution above, N is used2Thoroughly remove oxygen.Azodiisobutyronitrile (AIBN) is added, hybrid system is sealed, perseverance is put into Tepidarium oscillator, after 50 DEG C of reaction a few hours, then brings up to 60 DEG C of reaction continuation reactions.Product uses second by being collected by centrifugation Alcohol is repeatedly washed, and removes unreacted reactant, then further washed with surname extraction.
The present invention is achieved through the following technical solutions：
A kind of high-performance and highly controllable hud typed trace sensor, the sensor is by amino functional titanium dioxide Silicon, Ag, imprinted layer are composited, and the Ag is carried on amino functional silica, form SiO2The nano combined grains of/Ag Son；The imprinted layer is by acrylamide (AM), GDMA (EGDMA) and azodiisobutyronitrile (AIBN) It is polymerized, the imprinted layer is coated on functionalization SiO2Outside/Ag nano-complex particles, the thickness of the imprinted layer for 40~ 170nm。
A kind of preparation method of high-performance and highly controllable hud typed trace sensor, step is as follows：
The preparation of step 1, amino functional Nano particles of silicon dioxide
Added into ethanol/water mixed solution under ammoniacal liquor, stirring condition, add TEOS, stirring adds APTES, continued Stirring reaction；Product is centrifuged, washing and drying, obtain amino functional Nano particles of silicon dioxide, it is stand-by；
Step 2, functionalization SiO2The preparation of/Ag nano-complex particles
Amino functional Nano particles of silicon dioxide is scattered in ethanol/water mixed solution, add silver nitrate solution and PVP solution, the magnetic agitation in dark surrounds；Then, EA is added, lifting temperature continues stirring reaction；Solid product is centrifuged Separation, is washed, and is dried, is obtained functionalization SiO2/ Ag nano-complex particles, it is stand-by；
Step 3, nucleocapsid SiO2/ Ag/MIPs preparation
By functionalization SiO2/ Ag nano-complex particles are distributed in acetonitrile, are added rhodamine 6G, AM and EGDMA, are used inertia Gas clean-up oxygen；Then, AIBN is added, sealing is placed in thermostatic control oscillator vibration, is set in 50 DEG C of progress prepolymerizations anti- Should, then heat to 60 DEG C and continue to react；Solid product is centrifuged, and is washed, and is dried, is obtained nucleocapsid SiO2/ Ag/MIPs, i.e., The high-performance and highly controllable hud typed trace sensor.
In step 1, the ethanol/water mixed solution, ammoniacal liquor, TEOS, APTES volume ratio are 80~100：10~20： 9：1~3；In the ethanol/water mixed solution, ethanol, the volume ratio of water are 4：5；The time of the stirring A is 4~6h, described The time for continuing stirring reaction is 10~14h.
In step 2, the amino functional Nano particles of silicon dioxide, ethanol/water mixed solution, silver nitrate solution, PVP Solution, EA amount ratio are 100mg：50mL：5mL：4~6mL：0.4~0.6mL；The concentration of the silver nitrate solution is 0.1mol/L, during the concentration of the PVP solution is 0.2mol/L, the ethanol/water mixed solution, ethanol, the volume ratio of water are 4：1；The time of the magnetic agitation in dark surrounds is 4~6h, and the time of the continuation stirring is 3~5h.
In step 3, the functionalization SiO2/ Ag nano-complex particles, acetonitrile, rhodamine 6G, AM, EGDMA, AIBN use Amount is than being 100mg：50~70mL：0.05~0.15mmol：0.3~0.5mmol：0.237~0.396mL：9~11mg；It is described Inert gas is nitrogen.
In step 1~3, described washing is ethanol and water and washed respectively 3 times.
Prepared high-performance and highly controllable hud typed trace sensor are used for selective absorption rhodamine 6G.
The similar synthetic method of preparation method of the corresponding non-imprinted polymer of the present invention as above, but is not added with R6G.
The technological merit of the present invention：
SERS technologies are combined by the present invention with surface molecule print technology so that the product of preparation has SERS detection skills concurrently The high sensitivity of art and MIT high selectivity；Present invention selection molecular imprinted polymer on surface (SMIPs) promotes tradition SERS linings The selectivity of bottom material, expands the application of SERS detections；In the present invention, shell can be controlled by changing dosage of crosslinking agent Thickness.In recent years, molecularly imprinted polymer (MIPs) received much concern.Because material avoids traditional MIPs inferior position, it can bind Template molecule, specific identification hole is combined it with SERS Detection Techniques, and the development to SERS technologies has highly important Meaning.The present invention represents SERS detection and had broad application prospects in new material technology field.
Brief description of the drawings
Fig. 1：The SiO of the different imprinted layer thickness of preparation2/ Ag/MIPs TEM image:40nm (a), 100nm (b), 170nm(c)；
Fig. 2：SiO2/ Ag/MPS and SiO2/ Ag/MIPs FFIR, curve a is SiO2/ Ag/MPS, Curve b is SiO2/Ag/MIPs；
Fig. 3：The SiO of different-thickness2/ Ag/MIPs absorption 10-6Mol/L R6G SERS detections：40nm (a), 100nm (b), 170nm (c)；
Fig. 4：SiO2/ Ag/MIPs absorption various concentrations R6G SERS spectra figure (a) and its raman scattering intensity and R6G concentration Linear relationship (b)；
Fig. 5：SiO2/ Ag/MIPs is 10–6SERS spectra selective enumeration method in mol/L R6G (a), RB (b) and CV (c).
Embodiment
With reference to specific implementation example, the present invention will be further described.
Embodiment 1：
(1) synthesis of amino functional Nano particles of silicon dioxide：
In 100mL single-necked flasks, 90mL ethanol, water mixed solvent (volume ratio 4 are added:5), and 15mL NH are added3· H2O.Under stirring condition, 9mLTEOS is added, 5h is persistently stirred.2mL APTES are added, continue to stir 12h.By product centrifugation point From washing and drying is stand-by.
(2) functionalization SiO2The synthesis of/Ag nano-complex particles：
In 100mL single-necked flasks, 100mg amino functional Nano particles of silicon dioxide is dispersed in 50mL ethanol/waters Mixed solvent (v/v=4:1) in, it is 0.1mol/L silver nitrate solutiones to add 5mL concentration, and being subsequently added 5mL concentration is 0.2mol/L PVP solution, continues the magnetic agitation 4h in dark surrounds.Then, 500 μ L EA are added, and temperature is brought up to 50 DEG C are continued to stir 5h.Centrifugation, unreacted reactant is removed with water and ethanol cyclic washing, final product vacuum at room temperature Dry.
(3) nucleocapsid SiO2/ Ag/MIPs preparation
In 100mL single-necked flasks, 100mg MPS are modified SiO2/ Ag nano-particles are dispersed in 60mL acetonitriles.Add 0.1mmol R6G, 0.4mmol AM and 237 μ L EGDMA, are passed through N at room temperature215 minutes, thoroughly remove oxygen.Then, 10mg AIBN are added, sealing is put into thermostatic control oscillator vibration, reaction temperature is set in 50 DEG C, reaction duration 6h.Then 60 DEG C are brought up to, then reacts 24h.The product that is collected by centrifugation and washed with ethanol, remove unreacted reactant, further use Surname extraction liquid is washed
In reaction system described in step (1), the volume ratio of TEOS and mixed solvent is 9mL:90mL, TEOS and ammoniacal liquor Volume ratio be 9mL:15mL, TEOS and APTES volume ratio are 9mL:2mL.Washing described in step, is second alcohol and water Wash 3 times respectively.
In reaction system described in step (2), silver nitrate solution and PVP volume ratio are 1mL:1mL, silver nitrate solution with The volume ratio of EA solution is 1mL:100μL.
In reaction system described in step (3), SiO2The mass volume ratio of/Ag nano-particles and acetonitrile solution is 100mg: 60mL, SiO2/ Ag nanoparticles and AIBN mass ratio are 100mg:10mg, SiO2The quality mole of/Ag nanoparticles and R6G solution Than for 100mg:0.1mmol, SiO2/ Ag nanoparticles are 100mg with the quality mol ratio of AM solution:0.4mmol, SiO2/ Ag nanometers Grain is 100mg with the mass volume ratio of EGDMA solution:237μL.Washing described in step, is ethanol and water washs 3 respectively It is secondary.
The similar synthetic method of preparation method of the corresponding non-imprinted polymer of the present invention as above, but is not added with R6G.
Embodiment 2：
(1) synthesis of amino functional Nano particles of silicon dioxide：
In 100mL single-necked flasks, 80mL ethanol, water mixed solvent (volume ratio 4 are added:5), and 10mL NH are added3· H2O.Under stirring condition, 8mL TEOS are added, 4h is persistently stirred.1mL APTES are added, continue to stir 10h.By product centrifugation point From washing and drying is stand-by.
(2) functionalization SiO2The synthesis of/Ag nano-complex particles：
In 100mL single-necked flasks, 100mg amino functional Nano particles of silicon dioxide is dispersed in 50mL ethanol/waters Mixed solvent (v/v=4:1) in, it is 0.1mol/L silver nitrate solutiones to add 4mL concentration, and being subsequently added 4mL concentration is 0.2mol/L PVP solution, continues the magnetic agitation 3h in dark surrounds.Then, 400 μ L EA are added, and temperature is brought up to 50 DEG C are continued to stir 4h.Centrifugation, unreacted reactant is removed with water and ethanol cyclic washing, final product vacuum at room temperature Dry.
(3) nucleocapsid SiO2/ Ag/MIPs preparation
In 100mL single-necked flasks, 100mg MPS are modified SiO2/ Ag nano-particles are dispersed in 50mL acetonitriles.Add 0.05mmol R6G, 0.3mmol AM and 316 μ L EGDMA, are passed through N at room temperature215 minutes, thoroughly remove oxygen.With Afterwards, 9mg AIBN are added, sealing is put into thermostatic control oscillator vibration, reaction temperature is set in 50 DEG C, reaction duration 5h.With After bring up to 60 DEG C, then react 20h.The product that is collected by centrifugation and washed with ethanol, remove unreacted reactant, further make Washed with surname extraction liquid
In reaction system described in step (1), the volume ratio of TEOS and mixed solvent is 8mL:80mL, TEOS and ammoniacal liquor Volume ratio be 8mL:10mL, TEOS and APTES volume ratio are 8mL:1mL.Washing described in step, is second alcohol and water Wash 3 times respectively.
In reaction system described in step (2), silver nitrate solution and PVP volume ratio are 1mL:1mL, silver nitrate solution with The volume ratio of EA solution is 1mL:100μL.
In reaction system described in step (3), SiO2The mass volume ratio of/Ag nano-particles and acetonitrile solution is 100mg: 50mL, SiO2/ Ag nanoparticles and AIBN mass ratio are 100mg:9mg, SiO2The quality mol ratio of/Ag nanoparticles and R6G solution For 100mg:0.05mmol, SiO2/ Ag nanoparticles are 100mg with the quality mol ratio of AM solution:0.3mmol, SiO2/ Ag nanometers Grain is 100mg with the mass volume ratio of EGDMA solution:316μL.Washing described in step, is ethanol and water washs 3 respectively It is secondary.
The similar synthetic method of preparation method of the corresponding non-imprinted polymer of the present invention as above, but is not added with R6G.
Embodiment 3：
(1) synthesis of amino functional Nano particles of silicon dioxide：
In 100mL single-necked flasks, 100mL ethanol, water mixed solvent (volume ratio 4 are added:5), and 20mL is added NH3·H2O.Under stirring condition, 10mL TEOS are added, 6h is persistently stirred.3mL APTES are added, continue to stir 14h.By product Centrifuge, washing and drying is stand-by.
(2) functionalization SiO2The synthesis of/Ag nano-complex particles：
In 100mL single-necked flasks, 100mg amino functional Nano particles of silicon dioxide is dispersed in 50mL ethanol/waters Mixed solvent (v/v=4:1) in, it is 0.1mol/L silver nitrate solutiones to add 6mL concentration, and being subsequently added 6mL concentration is 0.2mol/L PVP solution, continues the magnetic agitation 5h in dark surrounds.Then, 600 μ L EA are added, and temperature is brought up to 50 DEG C are continued to stir 6h.Centrifugation, unreacted reactant is removed with water and ethanol cyclic washing, final product vacuum at room temperature Dry.
(3) nucleocapsid SiO2/ Ag/MIPs preparation
In 100mL single-necked flasks, 100mg MPS are modified SiO2/ Ag nano-particles are dispersed in 70mL acetonitriles.Add 0.15mmol R6G, 0.5mmol AM and 396 μ L EGDMA, are passed through N at room temperature215 minutes, thoroughly remove oxygen.With Afterwards, 11mg AIBN are added, sealing is put into thermostatic control oscillator vibration, reaction temperature is set in 50 DEG C, reaction duration 7h.With After bring up to 60 DEG C, then react 28h.The product that is collected by centrifugation and washed with ethanol, remove unreacted reactant, further make Washed with surname extraction liquid
In reaction system described in step (1), the volume ratio of TEOS and mixed solvent is 10mL:100mL, TEOS and ammonia The volume ratio of water is 10mL:20mL, TEOS and APTES volume ratio are 10mL:3mL.Washing described in step, is ethanol Washed respectively with water 3 times.
In reaction system described in step (2), silver nitrate solution and PVP volume ratio are 1mL:1mL, silver nitrate solution with The volume ratio of EA solution is 1mL:100μL.
In reaction system described in step (3), SiO2The mass volume ratio of/Ag nano-particles and acetonitrile solution is 100mg: 70mL, SiO2/ Ag nanoparticles and AIBN mass ratio are 100mg:11mg, SiO2The quality mole of/Ag nanoparticles and R6G solution Than for 100mg:0.15mmol, SiO2/ Ag nanoparticles are 100mg with the quality mol ratio of AM solution:0.5mmol, SiO2/ Ag receives The grain of rice is 100mg with the mass volume ratio of EGDMA solution:396μL.Washing described in step, is ethanol and water is washed respectively Wash 3 times.
The similar synthetic method of preparation method of the corresponding non-imprinted polymer of the present invention as above, but is not added with R6G.
Fig. 1：The SiO of preparation2/ Ag/MIPs TEM image.The Ag is carried on amino functional silica, is formed SiO2/ Ag nano-complex particles；The imprinted layer be by acrylamide (AM), GDMA (EGDMA) and Azodiisobutyronitrile (AIBN) is polymerized, and the imprinted layer is coated on functionalization SiO2It is described outside/Ag nano-complex particles The thickness of imprinted layer is 40~170nm.By in figure we can see that different imprinted layer thickness:40nm (a), 100nm (b), 170nm(c)；
Fig. 2：SiO2/ Ag/MPS and SiO2/ Ag/MIPs FFIR.By in figure we can see that print Mark polymer layer is successfully wrapped in SiO2/ Ag surfaces；
Fig. 3：The SiO of different-thickness2/ Ag/MIPs absorption 10-6Mol/L R6G SERS detections.By in figure we can see Go out the SiO of 40nm (a) polymer layer thickness2/ Ag/MIPs SERS maximum intensities, 100nm (b) takes second place, and 170nm (c) is minimum；
Fig. 4：SiO2/ Ag/MIPs absorption various concentrations R6G SERS spectra figure (a) and its raman scattering intensity and R6G concentration Linear relationship (b).By in figure we can see that in 1505cm-1Place, is gradually reduced with R6G concentration, and raman scattering intensity reduces, and two Person has functional relation；
Fig. 5：SiO2/ Ag/MIPs is 10–6SERS spectra selective enumeration method in mol/L R6G (a), RB (b) and CV (c). By in figure we can see that R6G peakedness ratio RB, CV are big.
Detectability evaluation is carried out by the following method in the specific embodiment of the invention：Adsorb various concentrations R6G institute Some SERS matrix all drops in slide, natural air drying.Excite 633nm, the time for exposure 10s of the spectra collection of each sample and The 0.25mW of incident laser power, SERS spectra, which is collected, uses 50 × nikon lens.With R6G concentration [c] for abscissa, SERS Intensity is that ordinate draws curve.
Test example 1：
SiO is have detected first2/ Ag/MIPs absorption various concentrations R6G raman scattering intensity then investigated raman scattering intensity with Linear relationship between R6G concentration.Configuration 10-6-10-14Mol/L R6G solution, divides and takes 5 10mg SiO2/ Ag/MIPs is added Into centrifuge tube, be separately added into various concentrations R6G and adsorbed, it is to be adsorbed after the completion of substrate is dropped in into slide, natural air drying, It is placed under object lens, adjusts object lens, then detects the raman scattering intensity of solution.In 1505cm-1Place can be observed, with R6G concentration gradually Reduction, raman scattering intensity reduces, and the two has functional relation.
Test example 2：
SiO is investigated2/ Ag/MIPs is to R6G, RB and CV selectivity (as shown in figure 5, obtained SiO2/Ag/MIPs 10–6It is stronger to R6G selectivity under mol/L concentration, also selective to RB and CV but compare to weaker).By R6G, RB 10 are configured to CV-6Mol/L solution.10mL R6G, RB, CV solution and 10mg SiO are taken respectively2/ Ag/MIPs be added to from In heart pipe, adsorbed, it is to be adsorbed after the completion of substrate is dropped in into slide, natural air drying is placed under object lens, adjust object lens, so The raman scattering intensity of solution is detected afterwards.Can be that ordinate draws Raman curve with respect to raman scattering intensity using wavelength as abscissa.As a result table It is bright, SiO2/ Ag/MIPs has good selectivity to R6G.

Claims (7)

1. a kind of high-performance and highly controllable hud typed trace sensor, it is characterised in that the sensor is by amino work( Silica, Ag, imprinted layer can be changed to be composited, the Ag is carried on amino functional silica, form SiO2/Ag Nano-complex particle；The imprinted layer is by the polymerization of acrylamide, GDMA and azodiisobutyronitrile Into, the imprinted layer is coated on functionalization SiO2Outside/Ag nano-complex particles, the thickness of the imprinted layer is 40~170nm.
2. the preparation method of a kind of high-performance as claimed in claim 1 and highly controllable hud typed trace sensor, it is special Levy and be, step is as follows：
The preparation of step 1, amino functional Nano particles of silicon dioxide
Added into ethanol/water mixed solution under ammoniacal liquor, stirring condition, add TEOS, stirred A, add APTES, continue to stir Mix reaction；Product is centrifuged, washing and drying, obtain amino functional Nano particles of silicon dioxide, it is stand-by；
Step 2, functionalization SiO2The preparation of/Ag nano-complex particles
Amino functional Nano particles of silicon dioxide is scattered in ethanol/water mixed solution, silver nitrate solution is added and PVP is molten Liquid, the magnetic agitation in dark surrounds；Then, EA is added, lifting temperature continues stirring reaction；Solid product is centrifuged, Washing, dries, obtains functionalization SiO2/ Ag nano-complex particles, it is stand-by；
Step 3, nucleocapsid SiO2/ Ag/MIPs preparation
By functionalization SiO2/ Ag nano-complex particles are distributed in acetonitrile, are added rhodamine 6G, AM and EGDMA, are used inert gas Remove oxygen；Then, AIBN is added, sealing is placed in thermostatic control oscillator vibration, is set in 50 DEG C of progress prepolymerizations, so After be warming up to 60 DEG C continue react；Solid product is centrifuged, and is washed, and is dried, is obtained nucleocapsid SiO2/ Ag/MIPs, i.e., described height Performance and highly controllable hud typed trace sensor.
3. the preparation method of high-performance as claimed in claim 2 and highly controllable hud typed trace sensor, its feature exists In in step 1, the ethanol/water mixed solution, ammoniacal liquor, TEOS, APTES volume ratio are 80~100：10~20：9：1~ 3；In the ethanol/water mixed solution, ethanol, the volume ratio of water are 4：5；The time of the stirring A is 4~6h, the continuation The time of stirring reaction is 10~14h.
4. the preparation method of high-performance as claimed in claim 2 and highly controllable hud typed trace sensor, its feature exists In, in step 2, the amino functional Nano particles of silicon dioxide, ethanol/water mixed solution, silver nitrate solution, PVP solution, EA amount ratio is 100mg：50mL：5mL：4~6mL：0.4~0.6mL；The concentration of the silver nitrate solution is 0.1mol/L, The concentration of the PVP solution is 0.2mol/L, in the ethanol/water mixed solution, and ethanol, the volume ratio of water are 4：1；It is described The time of magnetic agitation is 4~6h in dark surrounds, and the time of the continuation stirring is 3~5h.
5. the preparation method of high-performance as claimed in claim 2 and highly controllable hud typed trace sensor, its feature exists In, in step 3, the functionalization SiO2/ Ag nano-complex particles, acetonitrile, rhodamine 6G, AM, EGDMA, AIBN amount ratio For 100mg：50~70mL：0.05~0.15mmol：0.3~0.5mmol：0.237~0.396mL：9~11mg；The inertia Gas is nitrogen.
6. the preparation method of high-performance as claimed in claim 2 and highly controllable hud typed trace sensor, its feature exists In in step 1~3, described washing is ethanol and water and washed respectively 3 times.
7. the high-performance and highly controllable hud typed trace sensor described in claim 1 are used for selective absorption rhodamine 6G Purposes.
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