CN1069409C - Preparation of metal ion sensor - Google Patents
Preparation of metal ion sensor Download PDFInfo
- Publication number
- CN1069409C CN1069409C CN 96114793 CN96114793A CN1069409C CN 1069409 C CN1069409 C CN 1069409C CN 96114793 CN96114793 CN 96114793 CN 96114793 A CN96114793 A CN 96114793A CN 1069409 C CN1069409 C CN 1069409C
- Authority
- CN
- China
- Prior art keywords
- phosphatide
- present
- ion sensor
- metal ion
- ion
- Prior art date
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- Expired - Fee Related
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Composite Materials (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention belongs to the preparation method for a metal ion sensor. The present invention aims to prepare the metal ion sensor with good bio-compatibility and good stability by depositing phosphatide on a modified electrode of a thiol self-assembly monomolecular layer and embedding natural, or artificially synthesized bacterin in a phosphatide film. The ion sensor of the present invention improves the sensibility of detection from an inner amplification process and has the advantages of good organism compatibility, quick response, small size, wide dynamic range, good stability, etc. The Nernst response slope of the ion sensors to metal ions approaches to 60mV, the detection range is generally from 10<-7>M to 1M, and the service life is more than two months. The applications of the present invention in the life science aspects of medicine, clinic, etc. have greater superiority than these of the traditional ion-selective electrode.
Description
The invention belongs to the preparation method of metal ion sensor.
The detection of metallic ion has outstanding critical role in the field that environmental monitoring, metal industry, food inspection, clinical assay, medicine analysis etc. involve the interests of the state and the people, closely bound up with the people's life.Therefore, the development of metal ion sensor for many years is the major subjects that people study always, develops many metal ion sensors, particularly ion-selective electrode and has been widely used in commercial production and clinical medicine.But still there is the shortcoming that cannot overcome in traditional ion-selective electrode, the shortcoming such as serious and biocompatibility difference such as poor selectivity, memory effect, and they have seriously restricted its application in life science.
The objective of the invention is phosphatidosis on mercaptan self assembled monolayer modified electrode, in immobilized artificial membrane, embed rhzomorph natural or synthetic, the metal ion sensor of preparation good biocompatibility and good stability again.
The present invention will contain the organic solution that carbon chain length is 10～20 alkyl sulfhydryl through the gold electrode of chemistry and galvanochemistry cleaning treatment and dry mistake, comprise ethanol, methyl alcohol, acetone, tetrahydrofuran, dimethyl formamide, dimethyl acetamide and hexane, in soak the people after 1 minute to 2 days, form alkyl sulfhydryl unimolecular film modified electrode, clean repeatedly and nitrogen drying with absolute ethyl alcohol and high purity water again, with 0.1～1.0mg/ml phosphatide, comprise phosphatid ylcholine, oleic acid lecithin, two bay phatidylcholines, two palmityl choline, phosphatidyl-ethanolamine, phosphatidylserine, the chloroform of phosphatidylinositols and methyl alcohol mixed solution spread on the KSV5000 type LB membrane instrument water, kept static 10～40 minutes, slide sliding the barrier with 5～25mm/s speed again, when the surface pressure of phosphatide unimolecular layer is compressed to 40mN/m, sliding barrier stops to slide and keeping static, above-mentioned modified electrode is seen through the phosphatide unimolecular layer from gas phase to be entered the aqueous solution, with suction pipe phosphatide unnecessary on the water surface is sopped up, the electrode of then this having been modified phospholipid molecule immerses and contains mycin, comprise Nigeria's element, soaked in the solution of gramicidins and coban 5～20 hours, and made the biology sensor that certain metal ion species is had this special response of energy after the cleaning.When the ion in the substrate solution and rhzomorph interact, some electrical quantitys may take place, as the variation of current potential, electric current, resistance etc., produce one can detected conversion signal.
Ion transducer among the present invention is the sensitivity that improve to detect of amplification process internally, good biocompatible is arranged, advantages such as response is fast, size is little, wide dynamic range and good stability.These ion transducers approach 60mV to this special response slope of energy of metallic ion, and sensing range is generally 10
-7M～1M, the life-span period of greater than two months.Than traditional ion-selective electrode bigger superiority is arranged in application aspect medicine and the life science such as clinical.
Embodiment provided by the invention is as follows:
Embodiment 1.Preparation contains the K of valinomycins
+Sensor.
At first with chemistry and galvanochemistry cleaning treatment and under the high pure nitrogen atmosphere acetone soln of dry gold electrode immersion 2mM hexadecyl mercaptan, take out after 24 hours, clean repeatedly repeatedly and the high pure nitrogen drying with absolute ethyl alcohol and high purity water, with concentration be the volume ratio of 0.625mg/mL phosphatid ylcholine be 4: 1 chloroform and methyl alcohol mixed solution spread over KSV5000 type membrane instrument tank on the liquid level of redistilled water water, kept static 15 minutes, after treating organic solvent volatilization fully, slide sliding the barrier with 10mm/s speed, when the surface pressure of phosphatid ylcholine unimolecular layer was compressed to 40mN/m, sliding barrier stopped to slide and keeping static.With vertical czochralski method above-mentioned modified electrode being seen through the phosphatide unimolecular layer from gas phase again enters the aqueous solution, keep surface pressure and slide the barrier system being in steady state by computing machine in the process, deviation≤± 0.01mN/m, after electrode immerses solution, with suction pipe phosphatid ylcholine unnecessary on the water surface is sopped up, will modify good phosphatid ylcholine membrane electrode again and put into and contain 10
-3M NaCl and 10
-6Soak more than 6 hours in the solution of M valinomycins, promptly obtain the support phosphatid ylcholine film biology sensor of functionalization after the cleaning.It is as follows to metallic ion response order: Li
+＜Na
+＜Cs
+＜K
+＜Rb
+, to K
+This special response slope of energy approach 60mV, K
+To Na
+, Li
+, Ca
2+And Mg
2+Selectivity factor be respectively 10
-4, 10
-4, 2 * 10
-5With 3 * 10
-5K
+Detect the range of linearity from 10
-5M to 10
-1M is limited to 10 under detecting
-6M.Life-span period of greater than two months under-10 ℃.
Embodiment 2.Preparation contains the Na of coban
+Sensor.
The ethanolic solution of the gold electrode immersion 1.5mM tetradecyl mercaptan that will cross through chemistry and galvanochemistry cleaning treatment and nitrogen drying, take out after 20 hours, clean repeatedly repeatedly and the high pure nitrogen drying with absolute ethyl alcohol and high purity water, with concentration be the volume ratio of 0.75mg/ml lauroyl choline be 3: 1 chloroform and methyl alcohol mixed solution spread over KSV5000 type membrane instrument tank on the liquid level of redistilled water water, kept static 20 minutes, slide sliding the barrier with 12mm/s speed again, when the surface pressure of two bay phatidylcholine unimolecular layers is 45mN/m, sliding barrier stops to slide and keeping static, by vertical czochralski method above-mentioned modified electrode being seen through the phosphatide unimolecular layer from gas phase enters the aqueous solution, after electrode immerses solution, with suction pipe unnecessary two bay phatidylcholines on the water surface are sopped up, will modify two good bay phatidylcholine membrane electrodes again and put into and contain 10
-3M NaCl and 10
-6Soaked 10 hours in the solution of M coban, promptly be prepared into Na
+Sensor.This sensor is to Na
+This special response slope of energy be 60mV, detect the range of linearity from 10
-1M to 10
-5M is limited to 2 * 10 under detecting
-6M.Na
+To K
+, Rb
+And Ag
+The selection coefficient be respectively 2 * 10
-2, 6 * 10
-3With 32, through continuous coverage, do not find the existence of memory effect, compare biocompatibility with ion-selective electrode and be improved significantly.This sensor exists, and stores after two months down for-10 ℃, and performance does not change.
Claims (1)
1. the preparation method of a metal ion sensor, it is characterized in that gold electrode with chemistry and galvanochemistry cleaning treatment and dry mistake is immersed in contains in ethanol that carbon chain length is 10～20 alkyl sulfhydryl or the acetone soln after 1 minute to 2 days, clean repeatedly and nitrogen drying with absolute ethyl alcohol and high purity water, with 0.1～1.0mg/mL phosphatide, comprise that the chloroform of phosphatid ylcholine and two bay phatidylcholines and methyl alcohol volume ratio are that 4～3: 1 mixed solution spreads in the tank of KSV5000 type LB membrane instrument on the redistilled water water liquid level, kept static 10～40 minutes, slide sliding the barrier with 5～25mm/s speed again, when the surface pressure of phosphatide unimolecular layer is compressed to 40mN/m, stop to slide the barrier and keep static, above-mentioned modified electrode is seen through the phosphatide unimolecular layer from gas phase to be entered the aqueous solution, with suction pipe phosphatide unnecessary on the water surface is sopped up, next, the immobilized artificial membrane modified electrode is put into contained 10
-3M NaCl and 10
-6M valinomycins or 10
-3M NaCl and 10
-6Soaked in the solution of M coban 5～20 hours, and made Li after the cleaning
+, Na
+, Cs
+, K
+, Rb
+, Mg, Ca metallic ion have can this special linear response biology sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96114793 CN1069409C (en) | 1996-12-26 | 1996-12-26 | Preparation of metal ion sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96114793 CN1069409C (en) | 1996-12-26 | 1996-12-26 | Preparation of metal ion sensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1186237A CN1186237A (en) | 1998-07-01 |
| CN1069409C true CN1069409C (en) | 2001-08-08 |
Family
ID=5122304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96114793 Expired - Fee Related CN1069409C (en) | 1996-12-26 | 1996-12-26 | Preparation of metal ion sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1069409C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100522244C (en) * | 1995-06-07 | 2009-08-05 | 杜雷克特公司 | High viscosity liquid controlled delivery system |
| CN101713770B (en) * | 2008-10-06 | 2013-03-20 | 索尼株式会社 | A sensor for thiol analytes |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495038B (en) * | 2011-12-09 | 2013-06-12 | 南京大学 | Optical ion sensing film for detecting metal ions, and preparation method and application thereof |
| CN102539507B (en) * | 2011-12-19 | 2013-12-25 | 北京农业智能装备技术研究中心 | Method for evaluating nitrogen nutrition of paddy by micro dynamic ion flux technique and application thereof |
-
1996
- 1996-12-26 CN CN 96114793 patent/CN1069409C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100522244C (en) * | 1995-06-07 | 2009-08-05 | 杜雷克特公司 | High viscosity liquid controlled delivery system |
| CN101713770B (en) * | 2008-10-06 | 2013-03-20 | 索尼株式会社 | A sensor for thiol analytes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1186237A (en) | 1998-07-01 |
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