CN106932352A - A kind of method for determining activated sludge coefficient of stabilization - Google Patents
A kind of method for determining activated sludge coefficient of stabilization Download PDFInfo
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- CN106932352A CN106932352A CN201710243517.7A CN201710243517A CN106932352A CN 106932352 A CN106932352 A CN 106932352A CN 201710243517 A CN201710243517 A CN 201710243517A CN 106932352 A CN106932352 A CN 106932352A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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Abstract
The invention discloses a kind of method for determining activated sludge coefficient of stabilization, bimolecular reaction rate equation is primarily based onThe equation obtained after integrationThen the standard curve of absorbance A and activated sludge concentration c is drawn out by experiment, activated sludge solutions to be measured are often precipitated into 30s reads an absorption photometric numerical value and continuously read 5min, and calculates the activated sludge concentration c corresponding to absorption photometric number, obtains 1/c；In the case of electrolyte concentration in activated sludge colloidal solution is constant, according to the equation after integration1/c can be released in linear relation with time t, the straight slope is slow flocculation constant ks；Finally byTry to achieve the coefficient of stabilization W of activated Sludge System.Assay method of the present invention has the advantages that operating procedure is simple, time of measuring is short, certainty of measurement is high, and measured experimental result can reach required precision.
Description
Technical field
The present invention relates to a kind of method for determining activated sludge coefficient of stabilization, belong to the assay method neck of activated sludge parameter
Domain.
Background technology
In recent years, with developing rapidly for township enterprise and further speeding up for rural urbanization construction, various sanitary sewages,
The discharge of industrial wastewater increasingly increases, and activated sludge process has been widely applied to sewage treatment area, so as to be ground to microorganism
Study carefully and also increasingly paid attention to by environmentalist.W is a critically important function, and it represents sol system stability, is drawn
Microorganism field is used, is the flocculation relevant with electrolyte concentration and property for characterize the stability of microorganism system
Phenomenon, it is obtained by the ratio between two distinct types of flocculation constant, i.e.,krReferred to as Smoluchowski rapid flocculations are normal
Number, it is as caused by the Brownian movement of bacterium and the diffusion of decentralized mediumIts numerical value is normal by Boltzman
Number (k), the temperature (T) of system, the viscosity (η) of decentralized medium is determined；ksReferred to as slow flocculation constant, be and electrolyte concentration
The flocculation phenomenon relevant with property, its value determined by repulsion potential energy in dlvo theory, Shaw DJ in 1994《Colloid
and surface chemistry》The measure on coefficient of stabilization was illustrated in one book.The Liu Xiaomeng of China Science ＆ Technology University exists
This method is refer in his doctor's thesis, i.e., at the beginning of the purebred microorganism 2h turbidity kinetic curves as obtained by experiment
The slope in stage beginning is ksValue, doctor Liu Xiaomeng assembles and used during the mechanism of action extension DLVO reasons in microorganisms
By, and determine the coefficient of stabilization of microflora and carry out theory analysis with the change of incubation time, then with expansion DLVO theory, manage
Matched with experimental phenomena by analysis result, carry out the research of microbial flocculation performance with expansion DLVO theory so as to demonstrate
Reasonability.Also some people are by with ultramicroscope method, the result by counter or photography, being measured the reality of ion populations
Test.If colloidal sol is Quick Coagulation, velocity constant k does not change with electrolyte concentration C in colloidal sol, so W=1, if slow
Mutually exclusive potential energy V is there is between slow coagulation, particleR, so coagulation constant k is relevant with electrolyte concentration C.Various concentrations
Electrolyte just has different velocity constants, and the definition according to W just can obtain the W of various concentrations, and activated sludge solutions can be approximate
Regard colloidal solution as, substantial amounts of example shows that it is rational that activated sludge solutions are determined with the assay method of colloid.Using work
The electrical property and dlvo theory of property sludge suspension liquid, after making some approximate processings, obtain final product：LgW=-K1lgC+K2, C is electrolyte
Concentration.According to this formula it is concluded that：If not additional electrolyte solution, the coefficient of stabilization of activated sludge colloidal solution is constant
, according to the equation after integrationI.e.In linear relation with time t, its slope is slow flocculation constant ks,
When activated sludge sample absorbance to be measured is surveyed, an absorbance should be read every 30s, it is continuous to read to obtain ten groups of c in five minutes
Value, c10 adds primary sludge concentration c 0 c1, c2 to be designated as respectively ..., totally 11 groups of numerical value, just be can obtain by mappingWith the straight line of t
Slope.The Nacl solution of finite concentration gradient is added to obtain corresponding W in aforementioned manners again in activated sludge colloidal solution, will
Electrolyte concentration C takes the logarithm respectively with coefficient of stabilization W, and is mapped with lgW and lgC, and its slope is K1, just can test in this way
Card theory deduction formula 1gW=-K1lgC+K2Correctness.Meet linear relationship by lot of experiments checking 1gW and lgC, this
Illustrate that it is believable that the mechanism of Quick Coagulation and slow coagulation is envisioned.Slow coagulation is in lgW ＜ 4, i.e. W ＜ 10-4Feelings
Carried out under condition, by calculating for general typical sols VR≈ 15kT, i.e. potential barrier are 15kT, therefore to obtain a precipitability
The relatively good activated Sludge System of energy, should control condition of culture so that the potential barrier between thalline is less than 15kT, it is offset
The warm-up movement collision of particle.In addition it can further be stated that thalline straight line K of different sizes1And differ, because in coagulation mistake
Thalli granule can not possibly in an identical manner be assembled, precipitate in journey.And thalli granule distribution is also sufficiently complex, there is difference
The aggregation of size, some differences are constantly present with ideal model.
The content of the invention
Goal of the invention：The technical problems to be solved by the invention are to provide a kind of method for determining activated sludge coefficient of stabilization,
The method can accurately measure the coefficient of stabilization of activated sludge, reach experimental precision requirement.
In order to solve the above technical problems, the technical solution adopted in the present invention is：
A kind of method for determining activated sludge coefficient of stabilization, based on bimolecular reaction rate equationAfter integration
The equation for arrivingWherein, k is reaction rate constant；Absorbance A and activated sludge concentration c's are drawn out by experiment
Activated sludge solutions to be measured are often precipitated 30s and read an absorption photometric numerical value and continuously read 5min, and calculate suction by standard curve
Activated sludge concentration c corresponding to the light light number of degrees, obtains 1/c；The constant feelings of electrolyte concentration in activated sludge colloidal solution
Under condition, according to the equation after integration1/c can be released in linear relation with time t, the straight slope is slow
Flocculation constant ks；Finally byTry to achieve the coefficient of stabilization W of activated Sludge System.
The method of said determination activated sludge coefficient of stabilization, specifically includes following steps：
Step 1, draws the standard curve of activated sludge concentration c- absorbance As：
A. weighed after filter paper is dried, be designated as m1；
B. 100mL activated sludge is taken, after standing 30min；
C. the activated sludge after standing is filtered with filter paper, is weighed after drying, be designated as m2；
D. the concentration of the activated sludge is 10 × (m2-m1)；
E. the activated sludge of concentration known is divided into six groups carries out parallel test, and three groups of activated sludge of selection are diluted place
Reason, the concentration after dilution is respectively 3/4 times, 1/2 times and 1/4 times of original concentration, and two groups of activated sludge of selection are carried out at concentration
Reason, the concentration after concentration is 3/2 times and 2 times of original concentration, and remaining one group of activated sludge is not processed；Drawing absorbance A
During activated sludge concentration c standard curves the sludge concentration of six groups of standard liquids be respectively 3/4 times of primary sludge concentration, 1/2 times,
1/4 times, 1 times, 3/2 times and 2 times, such concentration is set makes curve wide coverage, it is ensured that sludge concentration to be measured falls in standard
On curve；
F. six groups of activated sludge after step e is processed are centrifuged respectively, cleaning treatment, are then re-suspended and are being gone
In ionized water, absorbance is surveyed after fully shaking up at 650nm under ultraviolet specrophotometer, activity is dirty under obtaining six groups of various concentrations
Absorbance of the mud at 650nm；When surveying absorbance at 650nm, reading is needed in time, rapidly, if data fluctuate up and down should read more
Several groups of data are averaged, if data reduce always should take maximum, activity are drawn after the absorbance of six groups of standard liquids has been surveyed
The standard curve of sludge concentration c- absorbance As；
G. the standard curve of activated sludge concentration c absorbance As is drawn；
Step 2, activated sludge sample to be measured is taken out, and is placed under 6500rpm after being centrifuged and cleaning, and is re-suspended and is being gone
In ionized water；
Activated sludge suspension after being suspended in step 3, step 2 reads absorbance at 650nm and is designated as A0, often precipitate 30s
Read a value again afterwards and be designated as Ai, it is continuous to read five minutes, until recorded A10, the activated sludge concentration c- suctions drawn by step 1
The standard curve of luminosity A is calculated and A0、A1。。。A10Corresponding c0、c1。。。c10Value；
Step 4, by c0、c1。。。c10Linear relationship inverted and that 1/c-t is drawn with time t, gained straight slope is slow
Slow flocculation constant ks；
Step 5, using formulaCalculate activated sludge coefficient of stabilization W.
Wherein, in step 5,Wherein, K is Boltzmann constant, and T is system temperature, and η is viscous for decentralized medium
Degree.
Wherein, in step 1a steps, filter paper is placed in baking oven in drying 2h at 105 DEG C.
Wherein, in step 1c steps, by the activated sludge after filtering in drying 8h at 105 DEG C.
Wherein, in step 1e steps, it is specifically to be carried out with the method for volumetric flask constant volume that activated sludge is diluted into treatment
Dilution, it is specifically to be concentrated with the method for volumetric flask constant volume that activated sludge is carried out into concentration.
Wherein, in step 1f steps, six groups of activated sludge are respectively placed under 6500rpm carries out centrifugal treating, at centrifugation
Cleaned with deionized water again after reason.
Compared with prior art, technical solution of the present invention have the advantage that for：
The present invention overcomes that measuring process present in existing method is cumbersome to the assay method of activated sludge coefficient of stabilization, surveys
Big, high to the measuring instrument requirement shortcoming of amount result error, assay method of the present invention has that operating procedure is simple, time of measuring
Short, certainty of measurement advantage high, measured experimental result can reach required precision.
Brief description of the drawings
Fig. 1 is the standard curve of activated sludge concentration c absorbance As in embodiment；
Fig. 2 is the image of the 1/c-t of activated sludge in reactor one in embodiment；
Fig. 3 is the image of the 1/c-t of activated sludge in reactor two in embodiment；
Fig. 4 is the image of the 1/c-t of activated sludge in reactor three in embodiment；
Fig. 5 is the image of the 1/c-t of activated sludge in reactor four in embodiment；
Fig. 6 is the image of the 1/c-t of activated sludge in reactor five in embodiment.
Specific embodiment
Technical scheme is described further below in conjunction with accompanying drawing, but the scope of protection of present invention is simultaneously
It is not limited to this.
Lot of experiments has verified that hydrodynamic condition influences whether every physiological property of activated sludge, wherein just wrapping
The coefficient of stabilization of activated sludge is included, under rational hydrodynamic gradient, the coefficient of stabilization of activated sludge can first be reduced and raised afterwards.
The present embodiment is divided into five sbr reactor devices, five aeration rates of sbr reactor device control respectively 40L/h, 80L/h,
100L/h, 120L/h and 140L/h, in addition to aeration rate difference, other conditions are consistent, and are controlled by the size of aeration rate
The hydrodynamic condition of measuring system；Five sludge concentrations of sbr reactor device are controlled in 3000mg/l, daily regular spoil disposal, treat anti-
Answer after the Activated Sludge Growth stabilization in device, the coefficient of stabilization of the activated sludge of each reactor is measured by sampling, concrete operations are as follows：
Step 1, draws the standard curve of activated sludge concentration c absorbance As, as shown in Figure 1；It is dirty that each reactor respectively takes 100ml activity
Mud mixed liquor, takes 7, the volumetric flask of 10ml, numbers respectively, and 1. number volumetric flask adds 2.5ml active sludge intermixtures；Number 2. hold
Measuring bottle adds 5ml active sludge intermixtures；3. number volumetric flask adds 7.5ml active sludge intermixtures；4. added in number volumetric flask
10ml active sludge intermixtures；15ml active sludge intermixtures are taken afterwards to outwell supernatant after being centrifuged under 6500rpm, and will
Activated sludge is moved into 5. number volumetric flask；20ml active sludge intermixtures are taken to outwell supernatant after being centrifuged under 6500rpm, and
Sludge is moved into 6. in number volumetric flask, plus pure water is to graduation mark and fully mixes；The sludge concentration of six volumetric flasks is respectively
750mg/l, 1500mg/l, 2250mg/l, 3000mg/l, 4500mg/l, 6000mg/l；Reference is done with pure water, extinction is being determined
The active sludge intermixture in volumetric flask must be shaken up before luminosity, the extinction light that five reactors make sample is determined respectively
Degree, in six standard samples of each reactor the ultraviolet light absorption luminosity of mixed liquor respectively may be about 0.284,0.554,0.86,1.14,
1.71、2.23；Step 2, the mud sample to be measured in five reactors is taken out, and mud sample to be measured should take the SBR at aeration end
Mixing sludge colloidal solution in reactor, each reactor takes three groups of testing samples, and every group of testing sample 10ml exists first
10min is centrifuged under 6500rpm, is washed with deionized twice, be then re-suspended in deionized water；Activity after suspension
Sludge suspension reads absorbance at once at 650nm, reads an absorbance every 30s afterwards, continuously reads 5min, each reactor pair
Data are answered as shown in table 1 below~table 5：
Table 1
Obtained by Fig. 2：ks=6.74；W=1.04 × 10-11。
Table 2
Obtained by Fig. 3：ks=11.41；W=6.13 × 10-12。
Table 3
Obtained by Fig. 4：ks=22.13；W=3.16 × 10-12。
Table 4
Obtained by Fig. 5：ks=12.58；W=5.56 × 10-12。
Table 5
Obtained by Fig. 6：ks=10.35；W=6.76 × 10-12。
By Fig. 2~Fig. 6, the activated sludge coefficient of stabilization for measuring is increased after first reducing, with experiment expected results
It is consistent, illustrates that this method is rational for determining activated Sludge System coefficient of stabilization.
Obviously, above-described embodiment is only intended to clearly illustrate example of the present invention, and is not to of the invention
The restriction of implementation method.For those of ordinary skill in the field, it can also be made on the basis of the above description
The change or variation of its multi-form.There is no need and unable to be exhaustive to all of implementation method.And these belong to this hair
Obvious change that bright spirit is extended out or among changing still in protection scope of the present invention.
Claims (7)
1. it is a kind of determine activated sludge coefficient of stabilization method, it is characterised in that：It is primarily based on bimolecular reaction rate equationThe equation obtained after integrationThen absorbance A and activated sludge concentration c's are drawn out by experiment
Activated sludge solutions to be measured are often precipitated 30s and read an absorption photometric numerical value and continuously read 5min, and calculate suction by standard curve
Activated sludge concentration c corresponding to the light light number of degrees, obtains 1/c；The constant feelings of electrolyte concentration in activated sludge colloidal solution
Under condition, according to the equation after integration1/c can be released in linear relation with time t, the straight slope is slow
Flocculation constant ks；Finally byTry to achieve the coefficient of stabilization W of activated Sludge System.
2. the method for determining activated sludge coefficient of stabilization according to claim 1, it is characterised in that specifically include following step
Suddenly：
Step 1, draws the standard curve of activated sludge concentration c- absorbance As：
A. weighed after filter paper is dried, be designated as m1；
B. 100mL activated sludge is taken, after standing 30min；
C. the activated sludge after standing is filtered with filter paper, is weighed after drying, be designated as m2；
D. the concentration c of the activated sludge is 10 × (m2-m1)；
E. the activated sludge of concentration known c is divided into six groups carries out parallel test, and three groups of activated sludge of selection are diluted treatment,
Concentration after dilution is respectively 3/4 times, 1/2 times and 1/4 times of original concentration, and two groups of activated sludge of selection carry out concentration,
Concentration after concentration is 3/2 times and 2 times of original concentration, and remaining one group of activated sludge is not processed；
F. six groups of activated sludge after step e is processed are centrifuged respectively, cleaning treatment, are then re-suspended in deionization
In water, absorbance is surveyed after fully shaking up at 650nm under ultraviolet specrophotometer, obtain under six groups of various concentrations activated sludge in
Absorbance at 650nm；
G. the standard curve of activated sludge concentration c- absorbance As is drawn；
Step 2, activated sludge sample to be measured is taken out, and is placed under 6500rpm after being centrifuged and cleaning, and is re-suspended in deionization
In water；
Activated sludge suspension after being suspended in step 3, step 2 reads absorbance at 650nm and is designated as A0, read again after often precipitating 30s
One value is designated as Ai, continuous to read five minutes, until recorded A10, the activated sludge concentration c- absorbance As drawn by step 1
Standard curve is calculated and A0、A1。。。A10Corresponding c0、c1。。。c10Value；
Step 4, by c0、c1。。。c10Linear relationship inverted and that 1/c-t is drawn with time t, gained straight slope is slow wadding
Solidifying constant ks；
Step 5, using formulaCalculate activated sludge coefficient of stabilization W.
3. it is according to claim 2 determine activated sludge coefficient of stabilization method, it is characterised in that：In step 5,
Wherein, K is Boltzmann constant, and T is system temperature, and η is the viscosity of decentralized medium.
4. it is according to claim 2 determine activated sludge coefficient of stabilization method, it is characterised in that：In step 1a steps, will
Filter paper is placed in baking oven in drying 2h at 105 DEG C.
5. it is according to claim 2 determine activated sludge coefficient of stabilization method, it is characterised in that：In step 1c steps, will
Activated sludge after filtering at 105 DEG C in drying 8h.
6. it is according to claim 2 determine activated sludge coefficient of stabilization method, it is characterised in that：In step 1e steps, will
It is specifically to be diluted with the method for volumetric flask constant volume that activated sludge is diluted treatment, and activated sludge is carried out into concentration tool
Body is concentrated with the method for volumetric flask constant volume.
7. it is according to claim 2 determine activated sludge coefficient of stabilization method, it is characterised in that：In step 1f steps, will
Six groups of activated sludge carry out centrifugal treating under being respectively placed in 6500rpm, are cleaned with deionized water again after centrifugal treating.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108931419A (en) * | 2018-07-18 | 2018-12-04 | 广西贺州市科隆粉体有限公司 | A kind of detection method of calcium carbonate powder activation grade |
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| CN1089658A (en) * | 1992-03-20 | 1994-07-20 | 郭敬慈 | BOD ultraviolet aeration rapid test method |
| CN102816797A (en) * | 2012-08-29 | 2012-12-12 | 哈尔滨工业大学 | Method for reinforcing flocculation performance of rhodopseudomonas faecalis of light-fermentative bacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108931419A (en) * | 2018-07-18 | 2018-12-04 | 广西贺州市科隆粉体有限公司 | A kind of detection method of calcium carbonate powder activation grade |
| CN108931419B (en) * | 2018-07-18 | 2021-03-23 | 广西贺州市科隆粉体有限公司 | Method for detecting activation degree of calcium carbonate powder |
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