CN106929538A - A kind of utilization biogas slurry backflow and the stalk anaerobic fermentation method of organic loading - Google Patents
A kind of utilization biogas slurry backflow and the stalk anaerobic fermentation method of organic loading Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
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Abstract
The invention discloses a kind of backflow of utilization biogas slurry and the stalk anaerobic fermentation method of organic loading, the step that it passes through mixing, anaerobic digestion, backflow, filtering, analysis keep sample, dry, by raw material using the multiple anaerobic fermentation of mode for flowing back, the present invention with mixed straw be single raw material, in semi batch reacor, different reflux ratios and organic loading are set, both influences to methane production and liquid-solid phase fermentation character during long-term ingestion are investigated;And by the change of dissolved organic matter in liquid phase, the mechanism that analysis operation parameter plays a role is so as to find optimal methane phase and fermentation condition so that the straw utilization rate of stalk anaerobic fermentation is maximized, when biogas engineering can be more efficient operation.
Description
Technical field
The invention belongs to stalk anaerobic fermentation method, and in particular to the stalk of a kind of utilization biogas slurry backflow and organic loading is detested
Aerobe fermentation method.
Background technology
Agricultural crop straw be always in biomass energy one of very potential raw material and anaerobic fermentation is to lack nothing
Under conditions of machine electron acceptor, produced the sour hydrogenesis and acetogenesis stage by hydrolysed ferment, the mistake of final generation carbon dioxide and methane
Journey, said process is considered as the most important approach of stalk resource, and due to stalk, nitrogen source lacks in itself, is often thrown in biogas engineering
Plus the raw material mixed fermentation such as excrement of animals is high to balance the organic pollution concentration such as carbon-nitrogen ratio, the biogas slurry ammonia nitrogen of generation, but due to
Biogas slurry complicated component, cropland application is also influenceed by factors such as land carrying capacity seasonal varieties, causes the biogas slurry can not be by
Fully dissolve, need further harmless treatment, so as to constrain the development of straw methane engineering, especially different reflux ratios and negative
The researches of stalk aerogenesis and fermentation character under the conditions of lotus, so as to limit application of the technique in engineering, it is therefore desirable to
A kind of scheme solves the above problems.
The content of the invention
It is an object of the invention to provide a kind of backflow of utilization biogas slurry and the stalk anaerobic fermentation method of organic loading.
Technical scheme is as follows:Using biogas slurry backflow and the stalk anaerobic fermentation method of organic loading, according to such as
Lower step：
（1）Mixing：Raw material 75% is mixed with sludge 25%, reactor is poured into after the completion of stirring；
（2）Anaerobic digestion：One is divided into 6 stages, the raw material before 2 hours add；Anaerobism is weighed during methane production
The important indicator of digestive efficiency, each stage methane production is had nothing in common with each other, and the organic loading of first stage is 1.5g/（L.d）, methane
Yield is slow, and the microorganism species epoch cycle more long to the environmental suitability and methane backeria of single stalk all can in reactor
Methane efficiency is had influence on, since second stage, reactor enters even running.5th stage OLR reaches 4g/（L.d）Backflow
Than being reduced to 1:1, methane maximum output reaches 970ml/（L.d）The average methane production in the stage is 768 ml/（L.d）.But
After OLR increases again, methane production drops to 587 ml/（L.d）.The change of unit stalk methane production be subject to reflux ratio and
The joint effect second stage TS methane productions of OLR have reached 194ml/g, but as OLR is gradually increased to 4g/（L.d）Produce first
Alkane amount drops to 152 ml/g on the contrary.It is 1 when biogas slurry reflux ratio is reduced under the conditions of same OLR:Methane production reaches when 1
202 maximum ml/g loads continue to improve, and unit stalk methane production begins to decline；
（3）Filtering：After feed liquid is absorbed to methane, is extruded with gauze and filtered, gas absorbs by 4mol/L NaOH solutions and obtains
The volume of methane；
（4）Analysis keeps sample：The biogas residue of filtering is taken out, the change of stalk each component is analyzed, biogas slurry keeps sample, and solid slag sample is in drying box
In dry 12h to permanent quality with 60 degree, use total organic carbon point for determining carbon-nitrogen ratio and each component fraction solid total organic carbon
Analyzer is determined, and kjeldahl nitrogen is former according to Fan Shi washing methods by the ratio of kelvin analysis-e/or determining cellulose hemicellulose and lignin
Reason is determined, and biogas slurry is by after 5000r/min centrifugations 15min, 10 times of measure ammonia nitrogen quality for being used for each index being diluted with ultra-pure water
After concentration adds 3mol/L phosphoric acid to be centrifuged using nessler reagent determination sample, determined using Shimadzu plus types, fiber mycin and wood
Glycan enzyme activity is determined on spectrophotometer using DNS methods, and dissolved organic matter quality concentration total organic carbon analyzer determines right
Afterwards dilute sample mass concentration to 10mg/L once, determine three-dimensional fluorescence using three-dimensional fluorescence spectrum instrument, parameter setting is：Excite
Wave-length coverage 200-500nm, launch wavelength 200-600nm, excite and are 4nm with transmite slit width, and length scanning interval is equal
It is 10nm, to avoid the occurrence of two grades of Rayleigh scatterings, the cut-off filter disc of 290nm is added in light extraction side；
（5）Dry：Biogas residue is carried out carbon dioxide and melts oxygen after being dried by drying box, secondary to use；
（6）Backflow：After biogas slurry after filtering is kept the skin wet according to reflux ratio, in Returning reactor.
The raw material is selected from one or more in rumen fluid, anaerobism mud, corn stalk, careless straw, rice straw, mixing straw.
The mixing speed is 80r/min.
The nylon gauze is 100 mesh nylon gauzes.
The drying box temperature is 60 degree.
Beneficial effects of the present invention：The present invention is single raw material with mixed straw, in semi batch reacor, sets different
Reflux ratio and organic loading, investigate both influences to methane production and liquid-solid phase fermentation character during long-term ingestion;And lead to
The change of dissolved organic matter in liquid phase is crossed, the mechanism that analysis operation parameter plays a role is so as to find optimal methane phase and fermentation
Condition so that the straw utilization rate of stalk anaerobic fermentation is maximized, when biogas engineering can be more efficient operation.
Brief description of the drawings
Fig. 1 is the graph of a relation of biogas reflux ratio of the invention and run time.
Fig. 2 is the graph of a relation of biogas reflux ratio of the invention and organic loading.
Fig. 3 is the ratio chart of volatile fatty acid of the invention.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1
As shown in Figure 1 to Figure 2, a kind of stalk anaerobic fermentation method of utilization biogas slurry backflow and organic loading, in accordance with the following steps
Carry out：
（1）Mixing：Raw material 75% is mixed with sludge 25%, reactor is poured into after the completion of stirring；
（2）Anaerobic digestion：One is divided into 8 stages, the raw material before 1 hour adds；Anaerobism is weighed during methane production
The important indicator of digestive efficiency, each stage methane production is had nothing in common with each other, and the organic loading of first stage is 1.5g/（L.d）, methane
Yield is slow, and the microorganism species epoch cycle more long to the environmental suitability and methane backeria of single stalk all can in reactor
Methane efficiency is had influence on, since second stage, reactor enters even running.5th stage OLR reaches 4g/（L.d）Backflow
Than being reduced to 1:1, methane maximum output reaches 970ml/（L.d）The average methane production in the stage is 768 ml/（L.d）.But
After OLR increases again, methane production drops to 587 ml/（L.d）.The change of unit stalk methane production be subject to reflux ratio and
The joint effect second stage TS methane productions of OLR have reached 194ml/g, but as OLR is gradually increased to 4g/（L.d）Produce first
Alkane amount drops to 152 ml/g on the contrary.It is 1 when biogas slurry reflux ratio is reduced under the conditions of same OLR:Methane production reaches when 1
202 maximum ml/g loads continue to improve, and unit stalk methane production begins to decline；
（3）Filtering：After feed liquid is absorbed to methane, is extruded with gauze and filtered；Gas absorbs by 4mol/L NaOH solutions and obtains
The volume of methane；
（4）Analysis keeps sample：The biogas residue of filtering is taken out, the change of stalk each component is analyzed, biogas slurry keeps sample；Solid slag sample is in drying box
In dry 12h to permanent quality with 60 degree, use total organic carbon point for determining carbon-nitrogen ratio and each component fraction solid total organic carbon
Analyzer is determined, and kjeldahl nitrogen is former according to Fan Shi washing methods by the ratio of kelvin analysis-e/or determining cellulose hemicellulose and lignin
Reason is determined, and biogas slurry is by after 5000r/min centrifugations 15min, 10 times of measure ammonia nitrogen quality for being used for each index being diluted with ultra-pure water
After concentration adds 3mol/L phosphoric acid to be centrifuged using nessler reagent determination sample, determined using Shimadzu plus types, fiber mycin and wood
Glycan enzyme activity is determined on spectrophotometer using DNS methods, and dissolved organic matter quality concentration total organic carbon analyzer determines right
Afterwards dilute sample mass concentration to 10mg/L once, determine three-dimensional fluorescence using three-dimensional fluorescence spectrum instrument, parameter setting is：Excite
Wave-length coverage 200-500nm, launch wavelength 200-600nm, excite and are 4nm with transmite slit width, and length scanning interval is equal
It is 10nm, to avoid the occurrence of two grades of Rayleigh scatterings, the cut-off filter disc of 290nm is added in light extraction side；
（5）Dry：Biogas residue is carried out carbon dioxide and melts oxygen after being dried by drying box, secondary to use；
（6）Backflow：After biogas slurry after filtering is kept the skin wet according to reflux ratio, in Returning reactor.
The mixing speed is 60r/min.
The drying box temperature is 80 degree
The methane production cycle is short of above-mentioned fermentation process, closest to arm's length standard, speed of production is fast, combustion period for methane content
Short, carbon dioxide content is low.
Embodiment 2
As shown in figure 3, a kind of stalk anaerobic fermentation method of utilization biogas slurry backflow and organic loading, is carried out in accordance with the following steps：
（1）Mixing：Raw material 60% is mixed with sludge 40%, reactor is poured into after the completion of stirring；
（2）Anaerobic digestion：One is divided into 6 stages, the raw material before 1 hour adds；Anaerobism is weighed during methane production
The important indicator of digestive efficiency, each stage methane production is had nothing in common with each other, and the organic loading of first stage is 1.5g/（L.d）, methane
Yield is slow, and the microorganism species epoch cycle more long to the environmental suitability and methane backeria of single stalk all can in reactor
Methane efficiency is had influence on, since second stage, reactor enters even running.5th stage OLR reaches 4g/（L.d）Backflow
Than being reduced to 1:1, methane maximum output reaches 970ml/（L.d）The average methane production in the stage is 768 ml/（L.d）.But
After OLR increases again, methane production drops to 587 ml/（L.d）.The change of unit stalk methane production be subject to reflux ratio and
The joint effect second stage TS methane productions of OLR have reached 194ml/g, but as OLR is gradually increased to 4g/（L.d）Produce first
Alkane amount drops to 152 ml/g on the contrary.It is 1 when biogas slurry reflux ratio is reduced under the conditions of same OLR:Methane production reaches when 1
202 maximum ml/g loads continue to improve, and unit stalk methane production begins to decline；
（3）Filtering：After feed liquid is absorbed to methane, is extruded with gauze and filtered；
（4）Analysis keeps sample：The biogas residue of filtering is taken out, the change of stalk each component is analyzed, biogas slurry keeps sample；
（5）Dry：Biogas residue is carried out carbon dioxide and melts oxygen after being dried by drying box, secondary to use；
（6）Backflow：After biogas slurry after filtering is kept the skin wet according to reflux ratio, in Returning reactor.
The change of organic loading concentration and reflux ratio containing, too high organic loading mass concentration not only react the product for producing acid
Methane it is unbalance, but also the process of anaerobic digestion can be suppressed.
Embodiment 3
A kind of utilization biogas slurry backflow and the stalk anaerobic fermentation method of organic loading, are carried out in accordance with the following steps：
（1）Mixing：Raw material 65% is mixed with sludge 35%, reactor is poured into after the completion of stirring；
（2）Anaerobic digestion：One is divided into 6 stages, the raw material before 1 hour adds；
（3）Filtering：After feed liquid is absorbed to methane, is extruded with gauze and filtered；
（4）Analysis keeps sample：The biogas residue of filtering is taken out, the change of stalk each component is analyzed, biogas slurry keeps sample；
（5）Dry：Biogas residue is carried out carbon dioxide and melts oxygen after being dried by drying box, secondary to use；
（6）Backflow：After biogas slurry after filtering is kept the skin wet according to reflux ratio, in Returning reactor.
The mixing speed is 70r/min.
The drying box temperature is 60 degree
The methane production cycle is short of above-mentioned fermentation process, speed of production is fast, and combustion period is short, and carbon dioxide content is low.
Embodiment 4
A kind of utilization biogas slurry backflow and the stalk anaerobic fermentation method of organic loading, are carried out in accordance with the following steps：
（1）Mixing：Raw material 85% is mixed with sludge 15%, reactor is poured into after the completion of stirring；
（2）Anaerobic digestion：One is divided into 4 stages, the raw material before 1 hour adds；
（3）Filtering：After feed liquid is absorbed to methane, is extruded with gauze and filtered；
（4）Analysis keeps sample：The biogas residue of filtering is taken out, the change of stalk each component is analyzed, biogas slurry keeps sample；
（5）Dry：Biogas residue is carried out carbon dioxide and melts oxygen after being dried by drying box, secondary to use；
（6）Backflow：After biogas slurry after filtering is kept the skin wet according to reflux ratio, in Returning reactor.
The mixing speed is 100r/min.
The drying box temperature is 60 degree
The methane production cycle of above-mentioned fermentation process is long, and speed of production is slow, and combustion period is long, and carbon dioxide content is high, and methane is accounted for
Compare many.
Claims (9)
1. the stalk anaerobic fermentation method of a kind of backflow of utilization biogas slurry and organic loading, it is characterised in that enter in accordance with the following steps
OK：（1）Mixing：Raw material 75% is mixed with sludge 25%, reactor is poured into after the completion of stirring；（2）Anaerobic digestion：One is divided into
It is 6 stages, the raw material before 2 hours add；The important indicator of anaerobic digestion efficiency, each rank are weighed during methane production
Section methane production is had nothing in common with each other, and the organic loading of first stage is 1.5g/（L.d）, methane production is slow, microorganism in reactor
The flora epoch cycle more long to the environmental suitability and methane backeria of single stalk can all have influence on methane efficiency, from second-order
Section starts, and reactor enters even running.
2. the 5th stage OLR reaches 4g/（L.d）Reflux ratio is reduced to 1:1, methane maximum output reaches 970ml/（L.d）The rank
The average methane production of section is 768 ml/（L.d）.
3. but after OLR increases again, methane production drops to 587 ml/（L.d）.
4. the change of unit stalk methane production is reached by the joint effect second stage TS methane productions of reflux ratio and OLR
194ml/g, but as OLR is gradually increased to 4g/（L.d）Methane production drops to 152 ml/g on the contrary.
5. it is 1 when biogas slurry reflux ratio is reduced under the conditions of same OLR:Methane production has reached 202 maximum ml/g and has born when 1
Lotus continues to improve, and unit stalk methane production begins to decline；（3）Filtering：After feed liquid is absorbed to methane, extruded with gauze
Filter；Gas absorbs the volume for obtaining methane by 4mol/L NaOH solutions；（4）Analysis keeps sample：Take out the biogas residue of filtering, analysis
The change of stalk each component, biogas slurry keeps sample；Solid slag sample dries 12h to permanent quality in drying box with 60 degree, for determining carbon
When each component fraction solid total organic carbon is determined nitrogen using total organic carbon analyzer, and kjeldahl nitrogen is fine by kelvin analysis-e/or determining
The ratio of the plain hemicellulose of dimension and lignin is determined according to Fan Shi washing methods principle, and biogas slurry is centrifuged 15min by 5000r/min
Afterwards, dilute 10 times of measure ammonia nitrogen mass concentrations for being used for each index with ultra-pure water and add 3mol/L using nessler reagent determination sample
After phosphoric acid centrifugation, determined using Shimadzu plus types, fiber mycin and xylanase activity are surveyed on spectrophotometer using DNS methods
Fixed, dissolved organic matter quality concentration total organic carbon analyzer measure and then dilute sample mass concentration once, make to 10mg/L
Three-dimensional fluorescence is determined with three-dimensional fluorescence spectrum instrument, parameter setting is：Excitation wavelength range 200-500nm, launch wavelength 200-
600nm, excites and is 4nm with transmite slit width, and length scanning interval is 10nm, to avoid the occurrence of two grades of Rayleigh scatterings,
The cut-off filter disc of 290nm is added in light extraction side；（5）Dry：Biogas residue is carried out carbon dioxide and melted after being dried by drying box
Oxygen, it is secondary to use；（6）Backflow：After biogas slurry after filtering is kept the skin wet according to reflux ratio, in Returning reactor.
6. the stalk anaerobic fermentation method of a kind of backflow of utilization biogas slurry and organic loading according to claim 1, its feature exists
In the raw material is selected from one or more in rumen fluid, anaerobism mud, corn stalk, careless straw, rice straw, mixing straw.
7. the stalk anaerobic fermentation method of a kind of backflow of utilization biogas slurry and organic loading according to claim 1, its feature exists
In the mixing speed is 80r/min.
8. the stalk anaerobic fermentation method of a kind of backflow of utilization biogas slurry and organic loading according to claim 1, its feature exists
In the nylon gauze is 100 mesh nylon gauzes.
9. the stalk anaerobic fermentation method of a kind of backflow of utilization biogas slurry and organic loading according to claim 1, its feature exists
In the drying box temperature is 60 degree.
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| CN111370071A (en) * | 2020-03-03 | 2020-07-03 | 重庆市环卫集团有限公司 | Method for recycling anaerobic biogas slurry of kitchen waste |
| CN112708639A (en) * | 2021-01-05 | 2021-04-27 | 北京化工大学 | Method for improving gas production efficiency of straw anaerobic digestion by coupling hydraulic retention time and feeding concentration |
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Application publication date: 20170707 |