CN106928324A - The method and reverse osmosis membrane of aquaporin expression activity in a kind of raising vesica - Google Patents
The method and reverse osmosis membrane of aquaporin expression activity in a kind of raising vesica Download PDFInfo
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- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/025—Reverse osmosis; Hyperfiltration
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- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
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Abstract
The invention discloses a kind of method for improving aquaporin expression activity in vesica, the method first step includes the phosphorylation of aquaporin and the preparation of phospholipid capsule bubble system solution；Second step is the introducing of phosphorylation aquaporin.The present invention also provides a kind of aquaporin reverse osmosis membrane, and it is adopted obtained phosphorylation aquaporin vesica with the aforedescribed process and prepares.The phosphorylation that the present invention passes through aquaporin, improve the expression activity of aquaporin, the aquaporin of the phosphorylation modification is embedded into vesica again, it is applied in reverse osmosis membrane, in the case where peer-level water flux and salt rejection rate is reached, the aquaporin and the ratio of vesica for being used are substantially reduced.
Description
Technical field
The present invention relates to water-treatment technology field, more particularly to a kind of side for improving aquaporin expression activity in vesica
Method and reverse osmosis membrane.
Background technology
At present, the purpose final to the research of aquaporin reverse osmosis membrane is to improve the water flux and salt rejection rate of film, and
This is closely bound up with aquaporin, and the loading of aquaporin, activity directly influence the performance of film, often aquaporin egg
White loading is bigger, active higher, then the performance of film is more excellent.However, in the reality of aquaporin reverse osmosis membrane
In preparation process, the loading and activity of aquaporin are well below theoretical level.Its reason is generally as follows：
The aquaporin limited amount that can be embedded in (1) vesica；
(2) because vesica is incomplete to the loading of aquaporin or adhesion is not enough consolidated and causes the stream of aquaporin
Lose；
(3) aquaporin contacts with basement membrane and reduces activity and even lose activity；
(4) work of aquaporin is reduced in vesica and membrane surface cohesive process because of the difference of membrane surface form
Property.
So, in order to improve the water flux and salt rejection rate of aquaporin reverse osmosis membrane it is necessary to from increase aquaporin
Loading and improve its expression activity start with.
However, the quantity of the aquaporin that can be embedded in a vesica is certain, therefore can only be from its table of raising
Achieved the goal up to activity.
Currently, Denmark Aquaporin A/S companies are in the technology of the aquaporin reverse osmosis membrane of merchandized handling
It is that aquaporin is directly embedded into vesica, is prepared by the method for interfacial polymerization.This preparation method has operation
Simply, prepared reverse osmosis membrane size is big, mechanical strength is good, is suitable for the advantage of industrialized production.
But, directly aquaporin is embedded into vesica, embedded quantity is few in itself for aquaporin, along with vesica pair
The loading of aquaporin is not exclusively or adhesion is not enough consolidated or aquaporin is contacted or membrane surface form pair with basement membrane
Effect of vesica etc. influences, and the last real aquaporin for playing active function is few, aquaporin reverse osmosis membrane
Performance do not reach film much in aquaporin all play its active level.
The content of the invention
It is an object of the invention to provide a kind of method for improving aquaporin expression activity in vesica and using thus making
Reverse osmosis membrane obtained in standby vesica, to improve the water flux and salt rejection rate of aquaporin expression activity and reverse osmosis membrane.
Technical scheme is as follows：
The method of aquaporin expression activity, comprises the following steps in a kind of raising vesica：
The first step includes the phosphorylation of aquaporin and the preparation of phospholipid capsule bubble system solution, and both do not have
Sequencing；Wherein, the phosphorylation method of aquaporin is as follows：
(1) the aquaporin solution of 0.5-5mg/mL is pipetted in volumetric bottle, is separately added into the glycerine of 10-200mL,
0.1mmol-5mmol protein kinases, 5-15mmol phosphate group donor agent, the agent of phosphate group donor and aquaporin mole
Ratio is 1: 20-1: 200, demarcated with the phosphate buffer that pH value is 7.5 to the volumetric bottle graduation mark of corresponding range, and prepare dense
Spend the aquaporin stock solution for 0.01-0.5mg/mL；
(2) the above-mentioned aquaporin stock solutions of 10-100ml are extracted, the calcium chloride solution of 0.005-0.1mol/L is added dropwise
0.1-1mL, and/or it is 6-7 that phosphoric acid is added dropwise to change the pH value of solution, and/or activate egg toward 0.5-5mL oxygen is passed through in solution
White kinases makes aquaporin that phosphorylation to occur；
(3) detergent is added in the aquaporin solution of phosphorylation, is centrifuged, remove clear liquid, leave aleuroplast, such as
This is 2-4 times repeatedly；
(4) retain the aleuroplast that centrifugation is obtained, be dissolved in the phosphate buffer that pH value is 7.5, it is cold at being placed in -20 DEG C
Jelly keeps in dark place；
The preparation method of phospholipid capsule bubble system solution is as follows：
By in amphiphilic lipids addition deionized water or phosphate buffer solution, ultrasonic vibration prepares phospholipid capsule bubble system solution,
Obtain the sample that concentration is 0.05-4mg/mL；
The introducing of second step, phosphorylation aquaporin
The aquaporin plastid that the first step is obtained mixes with the phospholipid capsule bubble system solution for preparing, and adds volume dense
Spend 10% glycerine 1-100mL, 0.005-0.1mg/mL n- octyl group-β-D- glucopyranoside 0.05-1mL, then by the mixed liquor
Being placed in carries out dialysis in phosphate buffer, period need to change phosphate buffer 2-5 times, obtains phosphorylation aquaporin vesica.
And preferably, the test of pure water transmission coefficient is carried out to phosphorylation aquaporin vesica to characterize its osmotically active.
Preferably, the aquaporin is selected from one or more in AQP1, AQP2, AQP4, AQP5, AQP6.
Preferably, the protein kinase be selected from PKA (PKA), protein kinase B (PKB), protein kinase C (PKC),
Serineprotein kinase, Serineprotein kinase, tryptophan protein kinase, phosphorylase kinase, pyruvic dehydrogenase kinase, ring
One or more in adenosine pka acid (cAMP), cyclic guanylic acid protein kinase (cGMP), histone kinase.
Preferably, phosphate group donor agent is selected from atriphos (ATP), GTP (GTP), diphosphonic acid bird
One or more in glycosides (GDP), ribonucleoside triphosphote (NTP).
Preferably, the detergent is selected from polyethylene glycol octadecyl ether, Triton X-100 and dodecyl
One or more in sodium sulphate, volumetric concentration is 0.05-O.5%, and addition is 0.05-1mL, and addition detergent makes protein
Subunit is easier migration.
Preferably, the amphiphilic lipids are selected from 1,2- DOPCs (DOPC), (2,3- dioleoyls-the third
Base)-trimethylamine (DOTAP), DOPS (DOPS), POPC (POPC), natural grease
One or more in matter extract, cuorin.
Preferably, the mol ratio of the amphiphilic lipids in the aquaporin plastid and vesica is 1: 10-1: 500.
The present invention also provides a kind of aquaporin reverse osmosis membrane, and it uses above-mentioned phosphorylation aquaporin vesica system
It is standby to obtain.
Preferably, the method for preparing aquaporin reverse osmosis membrane uses interfacial polymerization.
Compared with prior art, beneficial effects of the present invention are as follows：
Aquaporin is carried out phosphorylation modification by the present invention, improves its expression activity, and it is successfully embedded in into vesica afterwards
In, this modified aquaporin vesica is applied in reverse osmosis membrane, improve the water flux and salt rejection rate of film.
Certainly, implement any product of the invention to it is not absolutely required to while reaching all the above advantage.
Specific embodiment
The present invention improves the expression activity of aquaporin by the phosphorylation of aquaporin, then by the phosphorus
The aquaporin for being acidified modification is embedded into vesica, is applied in reverse osmosis membrane, is reaching peer-level water flux and desalination
In the case of rate, the aquaporin and the ratio of vesica for being used are substantially reduced；In other words, in the aquaporin for being used
In the case of the ratio identical of vesica, the water flux and salt rejection rate of reverse osmosis membrane are greatly improved.
Herein, the scope for being represented by " numerical value to another numerical value ", is that one kind avoids enumerating in the description
The summary representation of all numerical value in the scope.Therefore, the record of a certain special value scope, covers the number range
Interior any number and the relatively fractional value scope defined by any number in the number range, as bright in the description
Text writes out any number as should be compared with fractional value scope.
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate this hair
It is bright, rather than limit protection scope of the present invention.Technical staff makes according to the present invention in actual applications improvement and tune
It is whole, still fall within protection scope of the present invention.
Comparative example 1
As a comparison, first by the phospholipid capsule bubble system solution point of 0.1mg/mL aquaporins stock solution and 0.1mg/mL
Do not mix according to molar ratio 1: 100 and 1: 20, and be separately added into volumetric concentration for 10% glycerine 20mL, 0.01mg/mL n- are pungent
Base-β-D- glucopyranoside 0.1mL, then the mixed liquor is placed in dialysis is carried out in phosphate buffer, period changes phosphoric acid and delays
Fliud flushing 3 times, prepares aquaporin vesica, and the test of pure water transmission coefficient is carried out to obtained aquaporin vesica to characterize it
Osmotically active, the pure water transmission coefficient for obtaining is respectively 1456.21 × 10-6M/s and 3061.78 × 10-6m/s。
Embodiment 1
The aquaporin solution of 10mL 1mg/mL is pipetted in 100mL volumetric bottles, glycerine 10ml, 1mmol is separately added into
PKA and 0.5mmol cyclic adenosine monophosphate protein kinases, 10mmol ATP, with the phosphate buffer that pH value is 7.5 demarcate to
Graduation mark, prepares 0.1mg/mL aquaporin stock solutions.
The above-mentioned aquaporin stock solutions of 10ml are extracted, the calcium chloride solution 0.2ml of 0.01mol/L is added dropwise, and be added dropwise
The pH value of phosphorus acid-conditioning solution is 6.5, adds the polyethylene glycol octadecyl ether 0.1ml of volumetric concentration 0.1%, and centrifugation removes clear
Liquid, leaves aleuroplast, so repeatedly 4 times.
DOPC is pipetted, adds phosphate buffer solution, ultrasonic vibration 30min to prepare the phospholipid capsule bubble that concentration is 0.1mg/mL
System solution.
Aquaporin plastid and the phospholipid capsule bubble solution for preparing are mixed at 1: 100 in proportion, using with the phase of comparative example 1
Same method prepares phosphorylation aquaporin vesica, and the pure water transmission coefficient of obtained phosphorylation aquaporin vesica is
2593.09×10-6m/s。
Embodiment 2
The aquaporin solution of 10mL 1mg/mL is pipetted in 100mL volumetric bottles, glycerine 10mL, 1mmol is separately added into
PKA and 0.5mmol cyclic adenosine monophosphate protein kinases, 10mmol ATP, with the phosphate buffer that pH value is 7.5 demarcate to
Graduation mark, prepares 0.1mg/mL aquaporin stock solutions.The above-mentioned aquaporin stock solutions of 10ml are extracted, is added dropwise
The calcium chloride solution 0.2mL of 0.01mol/L, the pH value that phosphorus acid-conditioning solution is added dropwise is 6.5, adds the poly- second of volumetric concentration 0.1%
Glycol octadecyl ether 0.1mL, centrifugation, removes clear liquid, leaves aleuroplast, so repeatedly 4 times.Appropriate DOPC is pipetted, plus
Enter phosphate buffer solution, ultrasonic vibration 30min prepares the phospholipid capsule bubble system solution that concentration is 0.1mg/mL.By aquaporin egg
White matter body and the phospholipid capsule bubble solution for preparing mix at 1: 20 in proportion, and phosphorylation is prepared using with the identical method of comparative example 1
Aquaporin vesica, the pure water transmission coefficient of obtained phosphorylation aquaporin vesica is 3125.26 × 10-6m/s。
Embodiment 3
The aquaporin solution of 10mL 1mg/mL is pipetted in 100mL volumetric bottles, glycerine 10mL, 1mmol is separately added into
Protein kinase C and 0.5mmol cyclic adenosine monophosphate protein kinases, 10mmol ATP, with the phosphate buffer that pH value is 7.5 demarcate to
Graduation mark, prepares 0.1mg/mL aquaporin stock solutions.The above-mentioned aquaporin stock solutions of 10ml are extracted, is added dropwise
The calcium chloride solution 0.2mL of 0.01mol/L, the pH value that phosphorus acid-conditioning solution is added dropwise is 6.5, adds the poly- second of volumetric concentration 0.1%
Glycol octadecyl ether 0.1mL, centrifugation, removes clear liquid, leaves aleuroplast, so repeatedly 4 times.Appropriate DOPC is pipetted, plus
Enter phosphate buffer solution, ultrasonic vibration 30min prepares the phospholipid capsule bubble system solution that concentration is 0.1mg/mL.By aquaporin egg
White matter body and the phospholipid capsule bubble solution for preparing mix at 1: 100 in proportion, and phosphorylation is prepared using with the identical method of comparative example 1
Aquaporin vesica, the pure water transmission coefficient of obtained phosphorylation aquaporin vesica is 2658.28 × 10-6m/s。
Embodiment 4
The aquaporin solution of 10mL 1mg/mL is pipetted in 100mL volumetric bottles, the glycerine of 0.1mg/mL is separately added into
10mL, 1mmol PKA and 0.5mmol serinases, 10mmol ATP are demarcated with the phosphate buffer that pH value is 7.5
To graduation mark, 0.1mg/mL aquaporin stock solutions are prepared.The above-mentioned aquaporin stock solutions of 10ml are extracted, is added dropwise
The calcium chloride solution 0.2mL of 0.01mol/L, the pH value that phosphorus acid-conditioning solution is added dropwise is 6.5, adds the poly- second of volumetric concentration 0.1%
Glycol octadecyl ether 0.1mL, centrifugation, removes clear liquid, leaves aleuroplast, so repeatedly 4 times.Appropriate DOPS is pipetted, plus
Enter phosphate buffer solution, ultrasonic vibration 30min prepares the phospholipid capsule bubble system solution that concentration is 0.1mg/mL.By aquaporin egg
White matter body and the phospholipid capsule bubble solution for preparing mix at 1: 100 in proportion, and phosphorylation is prepared using with the identical method of comparative example 1
Aquaporin vesica, the pure water transmission coefficient of obtained phosphorylation aquaporin vesica is 2562.43 × 10-6m/s。
Embodiment 5
The aquaporin solution of 10mL 1mg/mL is pipetted in 100mL volumetric bottles, glycerine 10mL, 1mmol is separately added into
PKA and 0.5mmol cyclic adenosine monophosphate protein kinases, 10mmol ATP, with the phosphate buffer that pH value is 7.5 demarcate to
Graduation mark, prepares 0.1mg/mL aquaporin stock solutions.The above-mentioned aquaporin stock solutions of 10ml are extracted, is added dropwise
The calcium chloride solution 0.2mL of 0.01mol/L, the pH value that phosphorus acid-conditioning solution is added dropwise is 6.5, adds the poly- second of volumetric concentration 0.1%
Glycol octadecyl ether 0.1mL, centrifugation, removes clear liquid, leaves aleuroplast, so repeatedly 4 times.Pipette appropriate natural lipid
Extract, adds phosphate buffer solution, ultrasonic vibration 30min to prepare the phospholipid capsule bubble system solution that concentration is 0.1mg/mL.Will
Aquaporin plastid and the phospholipid capsule bubble solution for preparing mix at 1: 100 in proportion, using with the identical method system of comparative example 1
Standby phosphorylation aquaporin vesica, the pure water transmission coefficient of obtained phosphorylation aquaporin vesica is 2487.65 × 10- 6m/s。
Embodiment 6
The aquaporin solution of 10mL 1mg/mL is pipetted in 100mL volumetric bottles, glycerine 10mL, 1mmol is separately added into
PKA and 0.5mmol serinases, 10mmol ATP are demarcated to graduation mark with the phosphate buffer that pH value is 7.5,
Prepare 0.1mg/mL aquaporin stock solutions.The above-mentioned aquaporin stock solutions of 10ml are extracted, is added dropwise 0.01mol/L's
Calcium chloride solution 0.2mL, the pH value that phosphorus acid-conditioning solution is added dropwise is 6.5, adds the polyethylene glycol octadecyl of volumetric concentration 0.1%
Ether 0.1mL, centrifugation, removes clear liquid, leaves aleuroplast, so repeatedly 4 times.Appropriate natural lipid extract is pipetted, is added
Phosphate buffer solution, ultrasonic vibration 30min, it is the phospholipid capsule bubble system solution of 0.1mg/mL to prepare concentration.By aquaporin
Plastid and the phospholipid capsule bubble solution for preparing mix at 1: 100 in proportion, and phosphorylation water is prepared using with the identical method of comparative example 1
Channel protein vesica, the pure water transmission coefficient of obtained phosphorylation aquaporin vesica is 2607.59 × 10-6m/s。
The preparation embodiment of aquaporin reverse osmosis membrane
Phosphorylation aquaporin vesica obtained above is applied in reverse osmosis membrane, the permeability of the membrane energy is tested,
And be compared with the reverse osmosis membrane prepared without the aquaporin of phosphorylation modification, it is found that the water of non-phosphorylation modification leads to
When albumen and vesica ratio are 1: 20, film is in 25 DEG C, 5bar pressure, 500ppm chlorinations for reverse osmosis membrane prepared by road albumen
Sodium, under the test condition of the aqueous solution of pH=6.5, water flux and salt rejection rate are respectively 15.21LMH/bar and 99.35%；And phosphorus
The reverse osmosis membrane of aquaporin preparation of modification is acidified when albumen and vesica ratio are 1: 100, film is in 25 DEG C, 5bar
Pressure, 500ppm sodium chloride, under the test condition of the aqueous solution of pH=6.5, water flux and salt rejection rate are respectively 15.08LMH/
Bar and 99.33%.
The present invention carries out the phosphorylation modification without active somatic cell aquaporin to reach raising by artificial preparation raw material
In the purpose of aquaporin expression activity, and the aquaporin insertion amphiphilic lipids vesica that will successfully obtain, this is modified
Aquaporin vesica be applied in reverse osmosis membrane, so as to improve the water flux and salt rejection rate of reverse osmosis membrane.
Under the teaching of the present invention and above-described embodiment, those skilled in the art are easy to it is envisioned that the present invention is cited
Or each raw material for enumerating or its equivalent alterations, each processing method or its equivalent alterations can realize the present invention, and each original
The parameter bound value of material and processing method, interval value can realize the present invention, embodiment numerous to list herein.
Claims (9)
1. it is a kind of improve vesica in aquaporin expression activity method, it is characterised in that comprise the following steps：
The first step, including aquaporin phosphorylation and the preparation of phospholipid capsule bubble system solution；Wherein, aquaporin
Phosphorylation method it is as follows：
(1) the aquaporin solution of 0.5-5mg/mL is pipetted in volumetric bottle, is separately added into the glycerine of 10-200mL,
0.1mmol-5mmol protein kinases, 5-15mmol phosphate group donor agent, the agent of phosphate group donor and aquaporin mole
Ratio is 1: 20-1: 200, to be demarcated to volumetric bottle graduation mark with the phosphate buffer that pH value is 7.5, and compound concentration is 0.01-
The aquaporin stock solution of 0.5mg/mL；；
(2) the above-mentioned aquaporin stock solutions of 10-100mL are extracted, the calcium chloride solution 0.1- of 0.005-0.1mol/L is added dropwise
1mL, and/or it is 6-7 that phosphoric acid is added dropwise to change the pH value of solution, and/or to carry out activator protein sharp toward 0.5-5mL oxygen is passed through in solution
Enzyme makes aquaporin that phosphorylation to occur；
(3) detergent is added in the aquaporin solution of phosphorylation, is centrifuged, remove clear liquid, leave aleuroplast, it is so anti-
It is multiple 2-4 times；
(4) retain the aleuroplast that centrifugation is obtained, be dissolved in the phosphate buffer that pH value is 7.5, freezing keeps away at being placed in -20 DEG C
Light is preserved；
The preparation method of phospholipid capsule bubble system solution is as follows：
By in amphiphilic lipids addition deionized water or phosphate buffer solution, ultrasonic vibration prepares phospholipid capsule bubble system solution, obtains
Concentration is the sample of 0.05-4mg/mL；
The introducing of second step, phosphorylation aquaporin
The aquaporin plastid that the first step is obtained mixes with the phospholipid capsule bubble system solution for preparing, and adds volumetric concentration
10% glycerine 1-100mL, 0.005-0.1mg/mL n- octyl group-β-D- glucopyranoside 0.05-1mL, then the mixed liquor is put
Dialysis is carried out in phosphate buffer, period need to change phosphate buffer 2-5 times, obtain phosphorylation aquaporin vesica.
2. the method for improving aquaporin expression activity in vesica as claimed in claim 1, it is characterised in that the water leads to
Road albumen is selected from one or more in AQP1, AQP2, AQP4, AQP5, AQP6.
3. the method for improving aquaporin expression activity in vesica as claimed in claim 1, it is characterised in that the albumen
Kinases is selected from PKA, protein kinase B, protein kinase C, serineprotein kinase, Serineprotein kinase, tryptophan egg
White kinases, phosphorylase kinase, pyruvic dehydrogenase kinase, cyclic adenosine monophosphate protein kinase, cyclic guanylic acid protein kinase, histone
One or more in kinases.
4. the method for improving aquaporin expression activity in vesica as claimed in claim 1, it is characterised in that the phosphoric acid
Group donor agent is selected from one or more in atriphos, GTP, guanosine diphosphate (GDP), ribonucleoside triphosphote.
5. the method for improving aquaporin expression activity in vesica as claimed in claim 1, it is characterised in that the decontamination
Agent is selected from one or more in polyethylene glycol octadecyl ether, Triton X-100 and lauryl sodium sulfate, body
Product concentration is 0.05-0.5%, and addition is 0.05-1mL.
6. the method for improving aquaporin expression activity in vesica as claimed in claim 1, it is characterised in that the amphiphilic
Lipid is selected from 1,2- DOPCs, (2,3- dioleoyls-propyl group)-trimethylamine, DOPS, palm fibre
One or more in palmitic acid acyl oleoyl phosphatidylcholine, natural lipid extract, cuorin.
7. the method for improving aquaporin expression activity in vesica as claimed in claim 1, it is characterised in that the water leads to
The mol ratio of the amphiphilic lipids in road aleuroplast and vesica is 1: 10-1: 500.
8. a kind of aquaporin reverse osmosis membrane, it uses phosphorylation water obtained in any described method in claim 1-7
Channel protein vesica is prepared.
9. aquaporin reverse osmosis membrane as claimed in claim 8, it is characterised in that prepare aquaporin reverse osmosis membrane
Method uses interfacial polymerization.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102395357A (en) * | 2009-02-03 | 2012-03-28 | 阿夸兹有限公司 | Nanofabricated membrane using polymerized proteoliposomes |
| CN102802770A (en) * | 2009-06-19 | 2012-11-28 | 水通道蛋白有限公司 | Biometric membranes and uses thereof |
| CN105013334A (en) * | 2014-11-01 | 2015-11-04 | 中国海洋大学 | Preparation method for double-skin forward permeable membrane with aquaporin |
-
2017
- 2017-01-20 CN CN201710045069.XA patent/CN106928324A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102395357A (en) * | 2009-02-03 | 2012-03-28 | 阿夸兹有限公司 | Nanofabricated membrane using polymerized proteoliposomes |
| CN102802770A (en) * | 2009-06-19 | 2012-11-28 | 水通道蛋白有限公司 | Biometric membranes and uses thereof |
| CN105013334A (en) * | 2014-11-01 | 2015-11-04 | 中国海洋大学 | Preparation method for double-skin forward permeable membrane with aquaporin |
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