CN106924217A - The preparation method of the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection - Google Patents
The preparation method of the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection Download PDFInfo
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- CN106924217A CN106924217A CN201710095911.0A CN201710095911A CN106924217A CN 106924217 A CN106924217 A CN 106924217A CN 201710095911 A CN201710095911 A CN 201710095911A CN 106924217 A CN106924217 A CN 106924217A
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- 239000002086 nanomaterial Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 241000894006 Bacteria Species 0.000 title claims abstract description 13
- 230000003385 bacteriostatic effect Effects 0.000 title claims abstract description 13
- 238000001514 detection method Methods 0.000 title claims abstract description 13
- 229960003022 amoxicillin Drugs 0.000 claims abstract description 20
- LSQZJLSUYDQPKJ-NJBDSQKTSA-N amoxicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=C(O)C=C1 LSQZJLSUYDQPKJ-NJBDSQKTSA-N 0.000 claims abstract description 20
- LSQZJLSUYDQPKJ-UHFFFAOYSA-N p-Hydroxyampicillin Natural products O=C1N2C(C(O)=O)C(C)(C)SC2C1NC(=O)C(N)C1=CC=C(O)C=C1 LSQZJLSUYDQPKJ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 13
- 238000005253 cladding Methods 0.000 claims abstract description 7
- JLZUZNKTTIRERF-UHFFFAOYSA-N tetraphenylethylene Chemical group C1=CC=CC=C1C(C=1C=CC=CC=1)=C(C=1C=CC=CC=1)C1=CC=CC=C1 JLZUZNKTTIRERF-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229920002454 poly(glycidyl methacrylate) polymer Polymers 0.000 claims description 10
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- ZDOBFUIMGBWEAB-UHFFFAOYSA-N cucurbit[7]uril Chemical compound O=C1N(CN2C(=O)N3CN4C(=O)N5CN6C(=O)N7CN8C(=O)N9CN%10C(=O)N%11C%12)C%13N(C%14=O)CN(C%15=O)C2C3N%15CN(C2=O)C4C5N2CN(C2=O)C6C7N2CN(C2=O)C8C9N2CN(C2=O)C%10C%11N2CN2C(=O)N3C4C2N%12C(=O)N4CN1C%13N%14C3 ZDOBFUIMGBWEAB-UHFFFAOYSA-N 0.000 claims description 6
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000002105 nanoparticle Substances 0.000 claims description 5
- 229910052710 silicon Inorganic materials 0.000 claims description 5
- 239000010703 silicon Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 4
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims description 3
- 239000005977 Ethylene Substances 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 3
- 239000013049 sediment Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 101100346764 Mus musculus Mtln gene Proteins 0.000 claims description 2
- 230000009514 concussion Effects 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 claims description 2
- VPVSTMAPERLKKM-UHFFFAOYSA-N glycoluril Chemical compound N1C(=O)NC2NC(=O)NC21 VPVSTMAPERLKKM-UHFFFAOYSA-N 0.000 claims description 2
- 150000002678 macrocyclic compounds Chemical class 0.000 claims 1
- 150000001768 cations Chemical class 0.000 abstract description 6
- 239000002245 particle Substances 0.000 abstract description 6
- MSBXTPRURXJCPF-DQWIULQBSA-N cucurbit[6]uril Chemical class N1([C@@H]2[C@@H]3N(C1=O)CN1[C@@H]4[C@@H]5N(C1=O)CN1[C@@H]6[C@@H]7N(C1=O)CN1[C@@H]8[C@@H]9N(C1=O)CN([C@H]1N(C%10=O)CN9C(=O)N8CN7C(=O)N6CN5C(=O)N4CN3C(=O)N2C2)C3=O)CN4C(=O)N5[C@@H]6[C@H]4N2C(=O)N6CN%10[C@H]1N3C5 MSBXTPRURXJCPF-DQWIULQBSA-N 0.000 abstract description 4
- 230000003993 interaction Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 230000002776 aggregation Effects 0.000 abstract description 2
- 238000004220 aggregation Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000001988 small-angle X-ray diffraction Methods 0.000 description 3
- 241000209094 Oryza Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000005576 amination reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- -1 ethylenediamine Ester Chemical class 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 125000003055 glycidyl group Chemical group C(C1CO1)* 0.000 description 1
- VOZRXNHHFUQHIL-UHFFFAOYSA-N glycidyl methacrylate Chemical compound CC(=C)C(=O)OCC1CO1 VOZRXNHHFUQHIL-UHFFFAOYSA-N 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002411 thermogravimetry Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5138—Organic macromolecular compounds; Dendrimers obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/425—Thiazoles
- A61K31/429—Thiazoles condensed with heterocyclic ring systems
- A61K31/43—Compounds containing 4-thia-1-azabicyclo [3.2.0] heptane ring systems, i.e. compounds containing a ring system of the formula, e.g. penicillins, penems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
- A61K49/0069—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the agent being in a particular physical galenical form
- A61K49/0089—Particulate, powder, adsorbate, bead, sphere
- A61K49/0091—Microparticle, microcapsule, microbubble, microsphere, microbead, i.e. having a size or diameter higher or equal to 1 micrometer
- A61K49/0093—Nanoparticle, nanocapsule, nanobubble, nanosphere, nanobead, i.e. having a size or diameter smaller than 1 micrometer, e.g. polymeric nanoparticle
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/5115—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/5123—Organic compounds, e.g. fats, sugars
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1003—Carbocyclic compounds
- C09K2211/1007—Non-condensed systems
Abstract
The invention discloses a kind of preparation method of the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection, belong to nano material self-assembling technique field, it is using nanometer particle as kernel, interacted with the positive charge of cation high molecular by the carboxyl negative electrical charge on surface, cation high molecular in cladding, then host-guest interaction is passed through again, cladding cucurbituril derivative, acted on by positive and negative charge again, there is the tetraphenylethylene derivative of the carboxyl modified of aggregation luminescent effect in cladding, self-assembled supermolecular nano material is obtained.The present invention is simple and easy to do, with low cost, can be widely applied to the fields such as materialogy, biology, medical science, can both control the release of medicine Amoxicillin, controllable regulation bacteriostatic activity, and the Strength Changes detection bacterium for passing through fluorescence.
Description
Technical field
It is a kind of using positive and negative charge effect, in nanometer the invention belongs to nano material self-assembling technique field
Particle surface coats the nano-particle preparation method of cation high molecular and Cucurbituril [7] supermolecule.
Background technology
Layer-by-layer (LBL), is the principle using successively alternating deposit, by target compound in solution and base
The strong interaction (such as chemical bond) or weak interaction (such as electrostatic attraction, hydrogen bond, coordinate bond) of piece surface functional group,
Order about target compound structural integrity, stable performance are spontaneously formed and formed on matrix, with certain specific function film
A special kind of skill.
The layer-by-layer being related in the present invention is that the nanostructured to be formed is linked by non-bonding, including electrostatic
Gravitation, hydrogen bond action, Subjective and Objective tetra-inclusion complex.
The nano-particle prepared in the present invention, is using nanometer particle (MSN) as kernel, by the carboxyl on surface
Negative electrical charge interacts with the positive charge of cation high molecular, cation high molecular in cladding, then again by host-guest interaction,
Cladding cucurbituril derivative, can effectively suppress the toxic action of cation high molecular, control fungistatic effect.Meanwhile, then pass through
Positive and negative charge is acted on, the tetraphenylethylene derivative in cladding with the carboxyl modified of aggregation luminescent effect.
The content of the invention
The purpose of the present invention is directed to above-mentioned technical Analysis, there is provided a kind of with controllable bacteriostatic activity and Bacteria Detection
The preparation method of self-assembled supermolecular nano material, it is the preparation method process is simple, with low cost and easy to implement.
Technical scheme:
A kind of preparation method of the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection, it is described
Self-assembled supermolecular nano material is that Amoxicillin (AMO) is contained as core, in hole with carboxyl mesoporous silicon (MSN), and second is coated successively
Two amination poly (glycidyl methacrylate)s (PGEDA), Cucurbituril (CB [7]), the nano-particle of carboxylated tetraphenylethylene,
Preparation process is as follows：
1) by MSN dispersions in aqueous, it is stirred at room temperature, by the unnecessary Amoxicillin of mixture centrifugation removal, will
The sediment for obtaining is cleaned with water, and until can't detect Amoxicillin in supernatant, the mesoporous silicon for obtaining containing Amoxicillin is received
Rice corpuscles (AMO-MSN)；
2) above-mentioned MSN-AMO is dissolved in PBS, is separately added into the polymethyl acid glycidyl of ethylenediamine
Ester (PGEDA), Cucurbituril [7] (CB [7]), carboxylated tetraphenylethylene [TPE- (COOH)4] shaking table stirring, centrifugal water at room temperature
Wash, you can obtain self-assembled supermolecular nano material (MSN-PGEDA-CB [7]-TPE).
The poly (glycidyl methacrylate) (PGEDA) of the ethylenediamine is poly- by ethylene diamine-modified linear pattern
GMA (L-PGEDA)；The Cucurbituril [7] (CB [7]) is 7 big rings of glycoluril molecular composition point
Son, ethylenediamine is 100 with the mol ratio of poly (glycidyl methacrylate) (PGMA):1.
Further, step 1) in mixture turned into lower centrifugation in 200-8000 remove unnecessary A Moxi within 3-10 minute
Woods.
Further, step 2) in be with the PBS solution configuration concentration of pH 7.4 be 5mg mL-1PGEDA solution, take
State solution and add AMO-MSN, concussion, washing at room temperature obtains MSN-PGEDA；MSN-PGEDA is added in CB [7] solution,
Ultrasonic agitation, washes centrifugation and obtains MSN-PGEDA-CB [7] afterwards；Again by above-mentioned sample and TPE- (COOH)4It is added to pH
Stirred in 7.4 PBS solution, PBS solution washing is finally centrifuged, it is lyophilized to obtain MSN-PGEDA-CB [7]-TPE.
It is an advantage of the invention that：
The micella preparation method is simple is easy, with low cost, can be widely applied to the fields such as materialogy, biology, medical science,
Both the release of medicine Amoxicillin, controllable regulation bacteriostatic activity, and the Strength Changes detection bacterium for passing through fluorescence can have been controlled.
Brief description of the drawings
1) Fig. 1 is the design and the mechanism of action of self-assembled supermolecular anti-biotic material；
2) Fig. 2 is transmission electron microscope picture (a) MSN, (b) MSN-PGEDA, (c) MSN-PGEDA-CB [7], (d) MSN-PGEDA-
CB[7]-TPE
3) Fig. 3 is AMO, MSN-AMO, MSN-PGEDA, MSN-PGEDA-CB [7], and MSN-PGEDA-CB [7]-TPE
(a) wide-angle XRD；(b) small angle XRD；
4) Fig. 4 be CB [7], MSN-PGEDA-CB [7], TPE, and MSN-PGEDA-CB [7]-TPE infared spectrum；
5) Fig. 5 is the thermogravimetric curve of MSN, MSN-PGEDA, and MSN-PGEDA-CB [7].
Specific embodiment
The present invention will be further described below in conjunction with the accompanying drawings.
Accompanying drawing 1 gives the design and the mechanism of action of self-assembled supermolecular anti-biotic material of the present invention, from accompanying drawing 1, this
Invention contains Amoxicillin (AMO) as core, in hole with carboxyl mesoporous silicon (MSN), and ethylenediamine polymethylacrylic acid is coated successively
Ethylene oxidic ester (PGEDA), Cucurbituril (CB [7]), the nano-particle of carboxylated tetraphenylethylene obtains self-assembled supermolecular and receives
Rice material (MSN-PGEDA-CB [7]-TPE).
Instantiation of the present invention is given below to illustrate：
It is 3mg mL by concentration-1MSN and 3mg mL-1Amoxicillin disperses in aqueous, to be stirred at room temperature 2 days.
Mixture is centrifuged 3 minutes unnecessary Amoxicillins of removal under 8000 turns, the sediment that will be obtained is cleaned 3 times with water, until
Amoxicillin is can't detect in supernatant, product is obtained for AMO-MSN.
It is 5mg mL with the PBS solution configuration concentration of pH 7.4-1PGEDA solution, take above-mentioned solution 5mL and add AMO-
MSN, 200r shake 4h at room temperature, then wash 3 times and obtain MSN-PGEDA.MSN-PGEDA is added to 5mg mL-1CB [7] is molten
In liquid, ultrasonic agitation 2h washes centrifugation and obtains MSN-PGEDA-CB [7] afterwards.Again by the above-mentioned samples of 50mg and 2.5mg TPE-
(COOH)4It is added to and stirs in the PBS solution of pH7.4 30 minutes, PBS solution is finally centrifuged and washes 2 times afterwards, it is lyophilized to obtain MSN-
PGEDA-CB[7]-TPE。
Fig. 2 is transmission electron microscope picture (a) MSN of the present invention, (b) MSN-PGEDA, (c) MSN-PGEDA-CB [7], (d) MSN-
PGEDA-CB[7]-TPE。
The figure explanation：The particle size that transmission electron microscope picture can be seen that MSN is 125 ± 10nm, and can see surface
Meso-hole structure (a).After the load AMO and upper PGEDA of absorption, particle size is changed into 142 ± 12nm (b), afterwards MSN-
The particle diameter of PGEDA-CB [7] and MSN-PGEDA-CB [7]-TPE is respectively 158 ± 17 (c) and 161 ± 7nm (d).
Fig. 3 gives AMO of the present invention, MSN-AMO, MSN-PGEDA, MSN-PGEDA-CB [7], and MSN-PGEDA-CB
[7] XRD of-TPE, wherein (a) is wide-angle XRD；B () is small angle XRD.The figure explanation：Detect that MSN is carried using wide-angle XRD
Change before and after medicine, Amoxicillin shows many irregular diffraction maximums, but after it enters in MSN holes, only MSN
Diffraction maximum, and increasing with adsorption layer, the intensity of diffraction maximum reduces (a).In small angle XRD, MSN and MSN-PGEDA-
CB [7]-TPE shows regular XRD curves (b), it was demonstrated that meso-hole structure is not destroyed.In sum, Amoxicillin is loaded
Nanosized supramolecular material successfully builds.
Fig. 4 be CB [7], MSN-PGEDA-CB [7], TPE, and MSN-PGEDA-CB [7]-TPE infared spectrum.The figure is said
It is bright：The infared spectrum of MSN-PGEDA-CB [7] is in 1750cm-1Place shows obvious C=O absworption peaks, in 1790cm-1Place
Absworption peak is TPE- (COOH) in MSN-PGEDA-CB [7]-TPE4C=O key absworption peaks, infared spectrum can be seen that supermolecule
The successful structure of nano material.
Fig. 5 is the thermogravimetric curve of MSN, MSN-PGEDA, and MSN-PGEDA-CB [7].The figure explanation：By thermogravimetric analysis,
We can detect content of the absorption in each layer of mesoporous silicon face.Can by compare the different weightlessness of each sample come,
Finally we can draw every layer of content.
It should be noted that the above implementation method is only the preferred embodiment of the present invention, it is used only for being the present invention
Further illustrate, not thereby limit the scope of the present invention.Only obviously change to belonging to the technology of the present invention design
It is dynamic, equally within the scope of the present invention.
Claims (6)
1. a kind of preparation method of the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection, its feature
It is：The self-assembled supermolecular nano material is that Amoxicillin (AMO) is contained as core, in hole with carboxyl mesoporous silicon (MSN), successively
Cladding ethylenediamine poly (glycidyl methacrylate) (PGEDA), Cucurbituril [7] (CB [7]), carboxylated tetraphenylethylene
Nano-particle, preparation process is as follows：
1) by MSN dispersions in aqueous, it is stirred at room temperature, the unnecessary Amoxicillin of mixture centrifugation removal will obtains
Sediment cleaned with water, until can't detect Amoxicillin in supernatant, obtain containing the nanometer grain of Amoxicillin
Sub (AMO-MSN)；
2) above-mentioned MSN-AMO is dissolved in PBS, is separately added into the poly (glycidyl methacrylate) of ethylenediamine
(PGEDA), Cucurbituril [7] (CB [7]), carboxylated tetraphenylethylene [TPE- (COOH)4] shaking table stirring, centrifugal water at room temperature
Wash, you can obtain self-assembled supermolecular nano material (MSN-PGEDA-CB [7]-TPE).
2. the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection according to claim 1
Preparation method, it is characterized in that：The poly (glycidyl methacrylate) (PGEDA) of the ethylenediamine is by ethylene diamine-modified
Linear pattern poly (glycidyl methacrylate) (L-PGEDA).
3. the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection according to claim 1
Preparation method, it is characterized in that：The Cucurbituril [7] (CB [7]) is 7 macrocycle molecules of glycoluril molecular composition.
4. the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection according to claim 1
Preparation method, it is characterized in that：Ethylenediamine is 100 with the mol ratio of poly (glycidyl methacrylate) (PGMA):1.
5. the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection according to claim 1
Preparation method, it is characterized in that：Step 1) in mixture turned into lower centrifugation in 200-8000 remove unnecessary A Moxi within 3-10 minute
Woods.
6. the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection according to claim 1
Preparation method, it is characterized in that：Step 2) in be with the PBS solution configuration concentration of pH 7.4 be 5mg mL-1PGEDA solution, take
Above-mentioned solution adds AMO-MSN, and concussion, washing at room temperature obtains MSN-PGEDA；MSN-PGEDA is added to CB [7] solution
In, ultrasonic agitation is washed centrifugation and obtains MSN-PGEDA-CB [7] afterwards；Again by above-mentioned sample and TPE- (COOH)4It is added to pH
Stirred in 7.4 PBS solution, PBS solution washing is finally centrifuged, it is lyophilized to obtain MSN-PGEDA-CB [7]-TPE.
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| CN201710095911.0A CN106924217A (en) | 2017-02-22 | 2017-02-22 | The preparation method of the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection |
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| CN201710095911.0A CN106924217A (en) | 2017-02-22 | 2017-02-22 | The preparation method of the self-assembled supermolecular nano material with controllable bacteriostatic activity and Bacteria Detection |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502339A (en) * | 2017-07-19 | 2017-12-22 | 贵州大学 | A kind of ratio fluorescent probe for identifying nilotinib and its preparation and recognition methods |
| CN112592494A (en) * | 2020-10-31 | 2021-04-02 | 天津理工大学 | Preparation method of targeted colon part antibacterial imaging nano material based on dendritic cationic polyamide and tetraphenylethylene |
-
2017
- 2017-02-22 CN CN201710095911.0A patent/CN106924217A/en not_active Withdrawn
Non-Patent Citations (3)
| Title |
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| QINGLAN LI ET AL: "Mesoporous Silica Nanoparticles Coated by Layer-by-Layer Selfassembly Using Cucurbit[7]uril for in Vitro and in Vivo AnticancerDrug Release", 《AMERICAN CHEMICAL SOCIETY》 * |
| XIONGQI HAN ET AL.: "Aggregation-Induced-Emissive Molecule Incorporated into Polymeric Nanoparticulate as FRET Donor for Observing Doxorubicin Delivery", 《AMERICAN CHEMICAL SOCIETY》 * |
| YUANHAO WU ET AL.: "Layer-by-Layer (LBL) Self-Assembled Biohybrid Nanomaterials for Efficient Antibacterial Applications", 《AMERICAN CHEMICAL SOCIETY》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502339A (en) * | 2017-07-19 | 2017-12-22 | 贵州大学 | A kind of ratio fluorescent probe for identifying nilotinib and its preparation and recognition methods |
| CN107502339B (en) * | 2017-07-19 | 2019-10-11 | 贵州大学 | A kind of ratio fluorescent probe identifying nilotinib and its preparation and recognition methods |
| CN112592494A (en) * | 2020-10-31 | 2021-04-02 | 天津理工大学 | Preparation method of targeted colon part antibacterial imaging nano material based on dendritic cationic polyamide and tetraphenylethylene |
| CN112592494B (en) * | 2020-10-31 | 2022-08-02 | 天津理工大学 | Preparation method of targeted colon part antibacterial imaging nano material based on dendritic cationic polyamide and tetraphenylethylene |
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