CN106918705A - Detect test paper and its application of Fenpropathrin - Google Patents
Detect test paper and its application of Fenpropathrin Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a kind of test paper for detecting Fenpropathrin and its application.Test paper includes sample absorption pad (1), conjugate release pad (2), reaction film (3), adsorptive pads (4) and base plate (7), the sample absorption pad (1), conjugate release pad (2), reaction film (3), adsorptive pads (4) are pasted onto on base plate (7) successively, have on the reaction film (3) and be coated with the detection line (5) of Fenpropathrin hapten-carrier protein conjugate and be coated with the nature controlling line (6) of sheep anti mouse antiantibody, the conjugate release pad (2) is coated with Fenpropathrin monoclonal antibody colloid gold label thing.The test paper that the present invention is provided can be used for the residue detection of Fenpropathrin in tealeaves, and simple to operate, sensitivity is high, the detection short, low cost of required time, is adapted to the examination and on-site supervision of a large amount of samples.
Description
Technical field
The invention belongs to rapid detection technical field, and in particular to a kind of test paper of detection Fenpropathrin, its is particularly suitable
The detection of Fenpropathrin in Tea Samples.
Background technology
Fenpropathrin (Fenpropathrin), alpha-cyano-phenoxy benzyl -2,2,3,3- tetramethyl-ring propane carboxylic acids
Ester, also known as go out and sweep profit, it is a kind of pyrethroid series insecticidal/acaricidal agent.Fenpropathrin belongs to never poison, can act on insect
Nervous system, makes insect be overexcited, benumb and dead.In recent years, pyrethroid pesticide is because with deinsectization effective advantage
Commonly used, there is detection when Fenpropathrin therein is in food, Fenpropathrin is had moderate toxicity to higher mammal.
The detection of current Fenpropathrin residual is mainly liquid chromatogram, gas-chromatography and gas chromatography-mass spectrography etc., instrument
Method has the advantages such as the high, high specificity of detection sensitivity, but detection Sample pretreatment is cumbersome, time-consuming, and instrument is examined
Surveying needs expensive large-scale instrument and equipment, is equipped with the detection technique personnel of specialty, and its popularization and application is subject to a definite limitation.Colloid
Golden immunochromatographic method has that specific good, sensitivity is high, easy to operate, testing cost is low, it is excellent to be suitable for batch sample detection etc.
Point, is preferable quick screening means.
The content of the invention
It is an object of the invention to provide a kind of detection sensitivity is high, easy to operate, the short detection of low cost, detection time
The test paper of Fenpropathrin and its application.
The test paper of detection Fenpropathrin provided by the present invention, including sample absorption pad 1, conjugate release pad 2, reaction film
3rd, adsorptive pads 4 and base plate 7, the sample absorption pad 1, conjugate release pad 2, reaction film 3, adsorptive pads 4 are pasted onto base plate 7 successively
On, have on the reaction film 3 and be coated with the detection line 5 of Fenpropathrin hapten-carrier protein conjugate and be coated with goat-anti
The nature controlling line 6 of mouse antiantibody, the conjugate release pad 2 is coated with Fenpropathrin monoclonal antibody-colloid gold label thing.
The conjugate release pad 2 is stacked under sample absorption pad 1, and conjugate release pad 2 1/3 is capped on sample
Under product absorption pad 1.
The Fenpropathrin hapten-carrier protein conjugate is obtained by Fenpropathrin haptens and carrier protein couplet,
The carrier protein is bovine serum albumin(BSA), ovalbumin, hemocyanin or thyroprotein.
The Fenpropathrin haptens be by Fenpropathrin and m-hydroxybenzaldehyde, potassium cyanide, first cyanogen chrysanthemum acyl chlorides, to hydroxyl
Benzenpropanoic acid is synthesized and obtains, shown in shown Fenpropathrin haptens structural formula such as formula (I),
The Fenpropathrin monoclonal antibody is using Fenpropathrin hapten-carrier protein conjugate as immunogene system
It is standby to obtain；The sheep anti mouse antiantibody is obtained by mouse source antibody mediated immunity sheep.
It is a further object to provide a kind of preparation method of above-mentioned test paper, it is mainly included the following steps that：
A) the conjugate release pad 2 for being coated with Fenpropathrin monoclonal antibody-colloid gold label thing is prepared；
B) prepare to have and be coated with the detection line 5 of Fenpropathrin hapten-carrier protein conjugate and be coated with sheep anti mouse
The reaction film 3 of the nature controlling line 6 of antiantibody；
C) conjugate release pad 2, reaction film 3 and 7 groups of sample absorption pad 1, adsorptive pads 4 and the base plate that will a) and b) prepare
Dress up test paper.
The test paper of present invention detection Fenpropathrin is using the antibody antigen reaction of high degree of specificity and immunochromatographiassays assays skill
Art, Fenpropathrin monoclonal antibody-colloid gold label thing is fixed in conjugate release pad, and the Fenpropathrin in sample exists
In flow process, the Fenpropathrin monoclonal antibody-colloid gold label thing first and in conjugate release pad, formation medicine-anti-
Body-colloid gold label thing.The Fenpropathrin hapten-carrier protein conjugate on medicine and reaction film detection line in sample
Competition binding Fenpropathrin monoclonal antibody-colloid gold label thing, whether there is according to detection line red stripes or shade is sentenced
Fenpropathrin residual quantity in disconnected analyte sample fluid, 1mg/kg is limited to dark brownish green sample detection, and 2mg/ is limited to dry tea sample detection
kg。
During detection, sample is instilled in test paper hole clipping after treatment, when Fenpropathrin concentration in the sample is less than test limit
Or when being zero, Fenpropathrin monoclonal antibody-colloid gold label thing can be with the first cyanogen being fixed on reaction film in chromatography process
Chrysanthemum ester hapten-carrier protein couplet thing is combined, and respectively occurs a red stripes in detection line (T lines) and nature controlling line (C lines)；
If Fenpropathrin concentration in the sample is equal to or higher than test limit, Fenpropathrin monoclonal antibody-colloid gold label thing meeting
All combined with Fenpropathrin, so that because competitive reaction will not be with Fenpropathrin hapten-carrier albumen coupling at T lines
Thing is combined and occurs without red stripes.
Test paper of the invention has that sensitivity high, high specificity, low cost, simple to operate, detection time is short, storage is simple
The advantages of list, long shelf-life, applied widely, application easy to spread.
Brief description of the drawings
Fig. 1 is Fenpropathrin hapten synthesis route map of the present invention；
Fig. 2 Fenpropathrin haptens qualification figure (hydrogen spectrogram)；
Fig. 3 Fenpropathrin haptens qualification figure (carbon spectrogram)；
Fig. 4 is test paper cross-sectional view of the present invention；
Fig. 5 is detection paper result judgement figure of the present invention.
Specific embodiment
The present invention is expanded on further with reference to specific embodiment.It should be understood that these embodiments are merely to illustrate this
Invention, and it is not limited to the scope of the present invention.Reagent of the present invention unless otherwise instructed, is conventional reagent.
The preparation of the test paper of the detection Fenpropathrin of embodiment 1
The preparation method of the test paper mainly includes following steps：
A) the conjugate release pad 2 for being coated with Fenpropathrin monoclonal antibody-colloid gold label thing is prepared；
B) prepare to have and be coated with the detection line 5 of Fenpropathrin hapten-carrier protein conjugate and be coated with sheep anti mouse
The reaction film 3 of the nature controlling line 6 of antiantibody；
C) conjugate release pad 2, reaction film 3 and 7 groups of sample absorption pad 1, adsorptive pads 4 and the base plate that will a) and b) prepare
Dress up test paper.
Substep narration in detail below：
1st, the preparation of Fenpropathrin haptens, synthetic route is shown in Fig. 1
1) m-phenoxy benzene cyanalcohol is synthesized
1.22g m-hydroxybenzaldehydes are dissolved in 50mL tetrahydrofurans, ice bath stirring under be slowly added to 0.65g potassium cyanide and
0.76mL deionized waters, after continuing to stir 30min, are slowly added dropwise 2.1mL2mol/L concentrated hydrochloric acids, continue to stir 1h, the detection of TLC methods
Course of reaction, after reaction terminates, 50mL deionized waters is added toward reaction solution, is extracted with dichloromethane, and organic phase is through anhydrous slufuric acid
Sodium is dried, vacuum distillation removal solvent, obtains yellow oily liquid product；
2) hydroxyl benzal cyanhydrine connects first cyanogen chrysanthemum acyl chlorides between
Previous step products therefrom is dissolved in 50mL acetone, ice bath stirring cooling separately takes 3.54g first cyanogen chrysanthemum acyl chlorides and is dissolved in
50mL dichloromethane, is added in the acetone soln of above-mentioned product, adds 2.14mL pyrimidines, and mixture continues ice bath reaction
3h, after reaction completely, successively with 3mol/L hydrochloric acid and deionized water washing reaction liquid, with anhydrous sodium sulfate drying, vacuum distillation,
Obtain yellow oily liquid；
3) Fenpropathrin haptens is synthesized
Previous step product is dissolved in the 100mL98% concentrated sulfuric acids, 1.66g para hydroxybenzene propionic acid is added, 100 DEG C are heated to reflux
Reaction 6h, TLC detection, after reaction completely, reaction solution is poured into ice, and pH is adjusted to 5.0 with solid sodium bicarbonate, uses acetic acid second
Ester is extracted, anhydrous sodium sulfate drying, and product is purified with silica gel column chromatography, collects target components, is confirmed through proton nmr spectra, figure
Peak in 2 near 12ppm left sides is the hydrogen on haptens linking arm terminal carboxyl group, and the cluster peak near 7ppm is Fenpropathrin benzene
Hydrogen on ring hydrogen and cyano group α carbon, in carbon spectrogram shown in Fig. 3, the peak between 110-140ppm is for two on Fenpropathrin
Carbon on phenyl ring and cyano group, the peak on the left side is the carbon on linking arm terminal carboxyl group between 170-180ppm, and the peak on the right is first cyanogen
Carbon in chrysanthemum ester molecule on ester structure, shows hapten synthesis success.
2nd, immunogene preparation-Fenpropathrin haptens and bovine serum albumin(BSA) (BSA) coupling obtains immunogene
Weigh 40mg Fenpropathrin haptens to be dissolved in 3mL DMF solutions, (the two is molten to add 80mgEDC and 80mgNHS
In 3mL deionized waters) carry out activation 30min, will activation Fenpropathrin be added to 180~400mg BSA (be dissolved in 7mL go from
Sub- water) in carry out coupling and prepare immunogene, dialysed 3 days with 0.02mol/L phosphate buffers, change dialysis sooner or later daily
Liquid, to remove unreacted small-molecule substance, that is, obtains immunogene, packing, is saved backup in -20 DEG C.
3rd, the preparation of coating antigen-Fenpropathrin haptens and ovalbumin (OVA) coupling obtains coating antigen
20mg Fenpropathrin haptens 1.5mLDMF dissolvings are weighed, reaction solution I is obtained when being cooled to 10 DEG C；Take chloro-carbonic acid
The μ L of isobutyl ester 15 are added in reaction solution I, stirring reaction 30min under the conditions of 10 DEG C；45mg ovalbumins are taken, 3mL 50mmol/L are used
Na2CO3Dissolving, 10 DEG C of reaction 4h, shifts 4 DEG C of refrigerator overnights；Dialysed 3 days for 4 DEG C with 0.01mol/L phosphate buffers, to remove
Unreacted small-molecule substance, that is, obtain coating antigen, packing, is saved backup in -20 DEG C.
4th, the preparation of Fenpropathrin monoclonal antibody
1) animal immune
In the immunogene injection Balb/c Mice Bodies that step 2 is obtained, immunizing dose is 120 μ g/, its is produced anti-blood
Clearly.
2) cell fusion and cloning
After mice serum measurement result is higher, its splenocyte is taken, by 8:1 (quantitative proportion) ratio is thin with SP2/0 myeloma
Born of the same parents are merged, and cell supernatant, the positive hole of screening are determined using indirect competitive ELISA.Positive hole is carried out using limiting dilution assay
Cloning, the hybridoma cell strain until obtaining secreting Fenpropathrin monoclonal antibody.
3) cell cryopreservation and recovery：Monoclonal hybridoma strain is made 1 × 10 with frozen stock solution6The cell of individual/mL hangs
Liquid, preserves for a long time in liquid nitrogen.Cryopreservation tube is taken out during recovery, 37 DEG C of water-bath middling speeds is immediately placed in and is melted, after centrifugation removal frozen stock solution,
Move into culture culture in glassware.
4) production of monoclonal antibody and purifying：Hybridoma is placed in cell culture medium, is entered under the conditions of 37 DEG C
Row culture, the nutrient solution that will be obtained with octanoic acid-saturated ammonium sulfate method is purified, and obtains monoclonal antibody, -20 DEG C of preservations.
The cell culture medium is, to addition calf serum and sodium acid carbonate in RPMI1640 culture mediums, calf serum is existed
Final concentration of 20% (weight/mass percentage composition) in cell culture medium, makes sodium acid carbonate final concentration of in cell culture medium
0.2% (weight/mass percentage composition)；The pH of the cell culture medium is 7.4.
5th, the preparation of sheep anti mouse antiantibody
Using sheep as immune animal, pathogen-free domestic sheep is immunized by immunogene of mouse source antibody, obtains sheep anti mouse and resist
Antibody.
6th, the preparation of Fenpropathrin monoclonal antibody-colloid gold label thing
1) preparation of collaurum
1% gold chloride is diluted to 0.01% (weight/mass percentage composition) with double distilled deionized water, 100mL is taken and is placed in conical flask
In, boiling is heated to thermostatic electromagnetic agitator, the trisodium citrates of 2.5mL 1% are added under continuous high temperature, lasting stirring, after
It is continuous be at the uniform velocity heated with stirring to solution in it is bright red when stop, returning to original volume, 4 DEG C with deionized water after being cooled to room temperature
Preserve.The collaurum outward appearance for preparing is pure, bright, without precipitation and floating object.
2) preparation of Fenpropathrin monoclonal antibody-colloid gold label thing
Under magnetic stirring, the pH value of collaurum is adjusted to 7.2 with 0.2mol/LTris-Hcl solution, by every milliliter of collaurum
Add the standard of 40~80 μ g antibody to Fenpropathrin monoclonal antibody is added in colloidal gold solution in solution, continue to stir and evenly mix
30min, adds 10%BSA mixings, makes its final concentration of 1% (volumn concentration) in colloidal gold solution, stands
10min, 12000r/min, 4 DEG C of centrifugation 40min, abandon supernatant, and precipitation redissolution buffer solution is washed twice, and is initial with volume
The redissolution buffer solution of collaurum volume 1/10 will precipitate resuspended, put 4 DEG C it is standby.
Redissolve buffer solution：Containing bovine serum albumin(BSA) 0.1%~0.3% (volumn concentration), Tween-80 0.05%~
The 0.01mol/LTris-Hcl buffer solutions of 0.2% (weight/mass percentage composition), pH7.2.
7th, the preparation of conjugate release pad
Conjugate release pad is soaked in containing bovine serum albumin(BSA) (concentration of the bovine serum albumin(BSA) in buffer solution is
0.5%) with pH 7.2, in 0.5mol/L phosphate buffers, 1h is uniformly soaked, 37 DEG C of baking 3h are standby.Produced with hundred road companies
ZX1000 types spray film instrument, and the Fenpropathrin monoclonal antibody-colloid gold label thing that will be prepared uniformly is coated on conjugate release
On pad, after being coated with 0.01mL Fenpropathrins monoclonal antibody-colloid gold label thing per 1cm conjugates release pad, 37 DEG C of rings are placed in
Taken out after (humidity ＜ 20%) 60min in border, sealing is placed in dry environment (humidity ＜ 20%) and saves backup.Conjugate discharges
Mat material matter is nitrocellulose filter.
8th, the preparation of reaction film
Detection line 5 will be constituted in Fenpropathrin haptens-ovalbumin conjugate coating to reaction film, sheep anti mouse is resisted
Antibody is coated on composition nature controlling line 6 on reaction film.Reaction film is nitrocellulose filter.
Coating process：It is with 0.1mol/L pH7.4 phosphate buffers that Fenpropathrin haptens-ovalbumin conjugate is dilute
10mg/mL is released, ZX1000 type point film instruments are produced with hundred road companies, be coated in the detection line (T on nitrocellulose filter
Line), package amount is 1.0 μ g/cm2；Sheep anti mouse antiantibody is diluted to 200 μ with the phosphate buffer of 0.01mol/L, pH7.4
G/mL, the nature controlling line (C lines) on nitrocellulose filter is coated in point film instrument, and package amount is 1.0 μ g/cm2.To be coated with
Reaction film be placed in 37 DEG C under the conditions of dry 2h, it is standby.
9th, the preparation of sample absorption pad
Sample absorption pad is placed in containing 0.5% bovine serum albumin(BSA) (volumn concentration), pH7.2 0.1mol/L phosphoric acid
2h is soaked in salt buffer, 37 DEG C of baking 2h are standby.The material of sample absorption pad can be glass, non-woven fabrics, preferably hemofiltration film, nonwoven
Cloth.10th, the assembling of test paper, is shown in Fig. 4
Sample absorption pad 1, conjugate release pad 2, reaction film 3, adsorptive pads 4 are pasted onto on base plate 7 in order successively；Knot
Compound release pad 2 has 1/3 region to be absorbed by the sample pad 1 to cover from initiating terminal, end and the reaction film 3 of conjugate release pad 2
Top is connected, and the end of reaction film 3 is connected with the top of adsorptive pads 4, and the top of sample absorption pad 1 aligns with the top of base plate 7,
Alignd with the end of base plate 7 end of adsorptive pads 4；There are detection line 5 and nature controlling line 6, detection line 5 and Quality Control on the reaction film 3
Line 6 is the strip tape perpendicular with the length of the test paper；Detection line 5 is located at the side near the end of conjugate release pad 2；
Nature controlling line 6 is located remotely from the side of the end of conjugate release pad 2；Test paper machine is cut into 3mm small bars wide, mounted in special
Plastics fabrication in, form the test card with well and observation window, can be preserved 12 months under the conditions of 4~30 DEG C.
The detection of Fenpropathrin residual in the Tea Samples of embodiment 2
1. the pre-treatment of Tea Samples
1) sample should recover to 20~25 DEG C of room temperature before detecting；
2) in weighing 2.0 ± 0.05g samples to polystyrene centrifuge tube；
3) 10mL sample extraction liquid is added, is mixed with vortex instrument；；
4) dark brownish green and dry tea dilution process is as follows：
It is dark brownish green：The μ L sample dilutions of 100 μ L samples liquid+400；
Dry tea：The μ L sample dilutions of 100 μ L samples liquid+900；
Sample diluting liquid is 0.2moL/L phosphate buffers.
2nd, detected with the test paper of embodiment 1
Vertically dropwise addition 2~3 is dripped in well to draw Tea Samples solution to be checked with suction pipe, and liquid starts timing when flowing,
Reaction 5min, result of determination, it is invalid that other times judge.
3rd, testing result is analyzed, Fig. 5 is seen
Negative (-)：T lines and C lines all develop the color, and Fenpropathrin drug concentration is less than test limit in representing Tea Samples, such as schemes
5a。
Positive (+)：T lines develop the color without colour developing, C lines, and Fenpropathrin drug concentration is equal to or higher than test limit in representing sample,
Such as Fig. 5 b.
It is invalid：There are not C lines, show the deterioration failure of incorrect operating process or test paper, such as Fig. 5 c.In this situation
Under, retested using new test card.
4th, test limit experiment
Take blank dry tea sample, wherein respectively addition Fenpropathrin to final concentration of 1,2,4mg/kg, taking test paper is carried out
Detection, each sample is repeated three times.
During with detection paper Tea Samples, when wherein Fenpropathrin addition concentration is 1mg/kg, meat is shown on test paper
The red lines of visible two of eye, are negative；When wherein Fenpropathrin addition concentration is 2,4mg/kg, test paper nature controlling line shows
Show, but detection line does not show, is positive；The detection line for showing Fenpropathrin in this test paper dry tea sample is 2mg/kg.
Take the dark brownish green sample of blank, wherein respectively addition Fenpropathrin to final concentration of 0.5,1,2mg/kg, take test paper and enter
Row detection, each sample is repeated three times.
During sample dark brownish green with detection paper, when wherein Fenpropathrin addition concentration is 0.5mg/kg, shown on test paper
Macroscopic two red lines, are negative；When wherein Fenpropathrin addition concentration is 1,2mg/kg, test paper nature controlling line shows
Show, but detection line does not show, is positive；The detection line for showing Fenpropathrin in the dark brownish green sample of this test paper is 1mg/kg.
5th, false positive rate, false negative rate experiment
Take the dry tea positive sample of dark brownish green positive of the known Fenpropathrin content more than 1mg/kg, content more than 2mg/kg
Each 20 parts of product and content are less than each 20 parts of 2mg/kg dry tea negative samples less than the dark brownish green negative samples of 1mg/kg, content, with three batches
Test paper detected, calculates its false positive rate and false negative rate.Testing result is shown in Table 1~4.
Table 1 detects positive result
| Batch | Positive dark brownish green sample (20 parts) |
| 1 | 20 parts of positives |
| 2 | 20 parts of positives |
| 3 | 20 parts of positives |
Table 2 detects positive result
| Batch | Positive dry tea sample (20 parts) |
| 1 | 20 parts of positives |
| 2 | 20 parts of positives |
| 3 | 20 parts of positives |
Table 3 detects negative sample result
| Batch | Negative dark brownish green sample (20 parts) |
| 1 | 20 parts of feminine genders |
| 2 | 20 parts of feminine genders |
| 3 | 20 parts of feminine genders |
Table 4 detects negative sample result
| Batch | Negative dry tea sample (20 parts) |
| 1 | 20 parts of feminine genders |
| 2 | 20 parts of feminine genders |
| 3 | 20 parts of feminine genders |
During the detection paper positive produced with 3 batches, as a result it is all positive, it is known that positive coincidence rate is
100%, during the dark brownish green sample of 20 parts of feminine genders of detection, it is all negative, when detecting 20 parts of negative dry tea samples, only batch detection one
Part is positive.Illustrate that test paper of the present invention can be used for quickly detecting to Fenpropathrin in dark brownish green, dry tea.
6th, specific test
Specificity is commonly used cross reacting rate and is represented, refers to the energy that the antibody antigenic determinant different from structure combines
Power.By the sample of the often carbendazim of inspection, Triadimenol, the μ g/L of first bacterium miraculous cure thing 500, detected with test paper of the present invention.Result shows
Show, during with the carbendazim of the μ g/L of detection paper of the present invention 500, Triadimenol, first bacterium miraculous cure thing, test paper nature controlling line and detection line show
Color, is presented negative, illustrates this test paper to these medicine no cross reactions.
Claims (7)
1. a kind of test paper for detecting Fenpropathrin, including sample absorption pad (1), conjugate release pad (2), reaction film (3), water suction
Pad (4) and base plate (7), the sample absorption pad (1), conjugate release pad (2), reaction film (3), adsorptive pads (4) are pasted successively
On base plate (7), it is characterised in that there is the inspection for being coated with Fenpropathrin hapten-carrier protein conjugate on the reaction film
Survey line (5) and the nature controlling line (6) of sheep anti mouse antiantibody is coated with, the conjugate release pad (2) is coated with Fenpropathrin Dan Ke
Grand-colloid gold label thing.
2. test paper as claimed in claim 1, it is characterised in that the conjugate release pad (2) is stacked in sample absorption pad (1)
Under, and conjugate release pad (2) 1/3 is capped under sample absorption pad (1).
3. test paper as claimed in claim 1 or 2, it is characterised in that the Fenpropathrin hapten-carrier protein conjugate by
Fenpropathrin haptens is obtained with carrier protein couplet, and the carrier protein is bovine serum albumin(BSA), ovalbumin, hemocyanin
Or thyroprotein.
4. test paper as claimed in claim 3, it is characterised in that the Fenpropathrin haptens is by Fenpropathrin and a hydroxyl
Benzaldehyde, potassium cyanide, first cyanogen chrysanthemum acyl chlorides, para hydroxybenzene propionic acid are synthesized and obtain, and shown Fenpropathrin haptens structural formula is such as
Shown in formula (I),
5. test paper as claimed in claim 1 or 2, it is characterised in that the Fenpropathrin monoclonal antibody is with Fenpropathrin half
Antigen-carrier protein conjugate is prepared as immunogene, and the sheep anti mouse antiantibody is obtained by mouse source antibody mediated immunity sheep
's.
6. the preparation method of test paper described in a kind of any one of Claims 1 to 5, it is comprised the following steps：
A) the conjugate release pad (2) for being coated with Fenpropathrin monoclonal antibody-colloid gold label thing is prepared；
B) preparing has the detection line (5) that is coated with Fenpropathrin hapten-carrier protein conjugate and to be coated with sheep anti mouse anti-
The reaction film (3) of the nature controlling line (6) of antibody；
C) conjugate release pad (2), reaction film (3) and sample absorption pad (1), adsorptive pads (4) and the bottom that will a) and b) prepare
Plate (7) is assembled into test paper.
7. the method that Fenpropathrin is remained in the detection paper Tea Samples described in a kind of any one of application Claims 1 to 5, bag
Include following steps：
A) Tea Samples pre-treatment；
B) detected with the test paper described in any one of Claims 1 to 5；
C) testing result is analyzed.
Priority Applications (1)
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| CN109682961A (en) * | 2018-09-17 | 2019-04-26 | 北京勤邦生物技术有限公司 | A kind of test strips and its application detecting cyphenothrin |
| CN110540526A (en) * | 2019-08-09 | 2019-12-06 | 广西大学 | Carane acidyl 1,2, 3-triazole compound and preparation method and application thereof |
| CN111735951A (en) * | 2020-06-03 | 2020-10-02 | 北京勤邦生物技术有限公司 | Test strip for detecting fenpropathrin and application thereof |
| CN111812316A (en) * | 2020-06-03 | 2020-10-23 | 北京勤邦生物技术有限公司 | Application of fenpropathrin artificial antigen in enzyme linked immunosorbent assay kit |
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Cited By (8)
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| CN109682961A (en) * | 2018-09-17 | 2019-04-26 | 北京勤邦生物技术有限公司 | A kind of test strips and its application detecting cyphenothrin |
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| CN110540526A (en) * | 2019-08-09 | 2019-12-06 | 广西大学 | Carane acidyl 1,2, 3-triazole compound and preparation method and application thereof |
| CN110540526B (en) * | 2019-08-09 | 2022-08-30 | 广西大学 | Carane acidyl 1,2, 3-triazole compound and preparation method and application thereof |
| CN111735951A (en) * | 2020-06-03 | 2020-10-02 | 北京勤邦生物技术有限公司 | Test strip for detecting fenpropathrin and application thereof |
| CN111812316A (en) * | 2020-06-03 | 2020-10-23 | 北京勤邦生物技术有限公司 | Application of fenpropathrin artificial antigen in enzyme linked immunosorbent assay kit |
| CN111812316B (en) * | 2020-06-03 | 2022-11-18 | 北京勤邦生物技术有限公司 | Application of fenpropathrin artificial antigen in enzyme linked immunosorbent assay kit |
| CN111735951B (en) * | 2020-06-03 | 2022-11-18 | 北京勤邦生物技术有限公司 | Test strip for detecting fenpropathrin and application thereof |
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