CN106916879A - Mitochondrial genomes polymorphism builds cucumber finger-print and its application - Google Patents

Mitochondrial genomes polymorphism builds cucumber finger-print and its application Download PDF

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CN106916879A
CN106916879A CN201511003480.8A CN201511003480A CN106916879A CN 106916879 A CN106916879 A CN 106916879A CN 201511003480 A CN201511003480 A CN 201511003480A CN 106916879 A CN106916879 A CN 106916879A
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cucumber
polymorphism
mitochondria
dna
purity
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陈劲枫
赵宇
沈佳
李海梅
娄群峰
李季
钱春桃
张璐
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Nanjing Agricultural University
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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Abstract

Mitochondrial genomes polymorphism of the present invention builds cucumber finger-print and its application, it is related to Cucumber Mitochondria genomic marker to develop, mitochondrial genomes polymorphism mark screening is carried out between different cucumber varieties, the structure of mt DNA fingerprint collection of illustrative plates, and application of the mt DNA fingerprint collection of illustrative plates in terms of F1 Purity Identifications are hybridized --- Purity is carried out to " Nan Shui 3 " seed using polymorphism mark, belongs to biotechnology breeding field.The present invention builds 21 parts of mt DNA fingerprint collection of illustrative plates of cucumber variety such as " Chang Chun Mi Cis " based on Cucumber Mitochondria genome paternal inheritance characteristic first, first Application Cucumber Mitochondria genome polymorphism mark detection " Nan Shui 3 " cucumber seeds purity, identification, genetic relationship analysis for cucumber variety, the research such as classification and evolution provides a kind of new method, for paternal mitochondrial genomes do technique preparation in following the trail of crossover process, laid the foundation to formulate seedling purity early stage identification technology specification.

Description

Mitochondrial genomes polymorphism builds cucumber finger-print and its application
First, technical field
Mitochondrial genomes polymorphism of the present invention builds cucumber finger-print and its application belongs to biotechnology breeding field, is related to Huang Mitochondrial genomes polymorphism mark screening, mitochondrial DNA are carried out between melon mitochondrial genomes marker development, different cucumber varieties The structure of finger-print, and mt DNA fingerprint collection of illustrative plates is in hybridization F1Application in terms of Purity Identification --- using many State property mark carries out Purity to " Nan Shui 3 " seed.
2nd, background technology
DNA fingerprinting refers to the specific DNA fragment for being different from other kinds that a certain kind has, and its manifestation mode is one The difference of serial electrophoresis pattern, the heredity with many site property, high variability and simple and stable.The research of DNA fingerprinting, Cultivar identification, variety source evaluation and preservation for animal, plant and microorganism, genetic relationship analysis etc. have important Meaning.Mitochondria finger-print has same meaning, and for paternal inheritance kind, it can quick detection cenospecies Sub- purity, follows the trail of pollen contamination source.
Cucumber (Cucumis sativus L.) belongs to Curcurbitaceae Cucumis, annual herbaceous plant, in China existing 1500 Cultivation history for many years.During long-term natural selection, rich and varied cucumber cultivation type and kind is formd.According to It has been reported that Cucumber Mitochondria DNA is paternal inheritance, and short interspersed repetitive sequence accounts for the 13% of Cucumber Mitochondria genome, Theoretically mitochondrial markers development and application is laid a good foundation.
Amphilepsis characteristic based on cucumber Matrix attachment region, the part cucumber product that RAPD, AFLP, ISSR equimolecular mark build The finger-print planted has been reported.However, the Cucumber Mitochondria DNA built based on Cucumber Mitochondria genome paternal inheritance characteristic Finger-print has not been reported.
The present invention with 21 parts of cucumber varieties such as " Chang Chun Mi Ci " be material, using Cucumber Mitochondria genome exploitation mark, structure 21 parts of mt DNA fingerprint collection of illustrative plates of cucumber variety are built, has been laid the foundation to formulate seedling purity early stage identification technology specification.
3rd, the content of the invention
Technical problem
It is an object of the invention to provide a kind of method for building Cucumber Mitochondria DNA fingerprinting, to the mirror for cucumber variety Fixed, genetic relationship analysis, the research such as classification and evolution provides a kind of new method；And mt DNA fingerprint collection of illustrative plates exists Hybridization F1Application in terms of Purity Identification --- Purity is carried out to " Nan Shui 3 " seed using polymorphism mark, to The false cenospecies of other pollen sources and selfed seed on maternal south No. 2 plant of water are distinguished, it is that one kind is lost using Cucumber Mitochondria is paternal The seed purity identification method for passing characteristic and determining.
Technical scheme
The present invention is based on Cucumber Mitochondria genome paternal inheritance characteristic, builds Cucumber Mitochondria DNA fingerprinting, carries out Huang Melon cenospecies Purity Identification, its embodiment is as follows：
1. Cucumber Mitochondria genomic marker exploitation：
Compared with promitocondrion genomic data using Cucumber Mitochondria genome weight sequencing data, design Cucumber Mitochondria genome Primer, and the existing Cucumber Mitochondria genome SSR primers in Binding experiment room；
2. Cucumber Mitochondria genomic marker polymorphism screening：
1) DNA with 21 parts of cucumber varieties such as " Chang Chun Mi Ci " is template, is entered using following reaction system and PCR programs Row amplification, PCR reaction systems cumulative volume is 24 μ L：The μ L of 2 × Mix 12, sterilize ddH2O 9 μ L, forward and reverse 10 μm of ol L-1 Primer each 1 μ L, template 50ng μ L-1The μ L of DNA 1, are defined as after amplification program optimization：94 DEG C of predegeneration 2min, 94 DEG C Denaturation 20s, 68 DEG C of annealing 1min, 72 DEG C of extension 30s, totally 6 circulate, 94 DEG C of denaturation 20s, 58 DEG C of annealing 1min, 72 DEG C of extension 30s, totally 9 circulations, 94 DEG C of denaturation 20s, 50 DEG C of annealing 30s, 72 DEG C extend 30s, totally 13 circulations, 72 DEG C of extension 5min, 4 DEG C of preservations are entered performing PCR to 21 parts of cucumber samples and are expanded respectively with 81 pairs of mitochondria primers；
2) after PCR amplifications terminate, PCR amplifications are detected using 8% non-denaturing polyacrylamide gel, after 180v electrophoresis 3h Product, record data, collection electrophoretic image of taking pictures after silver staining colour developing；
3. Cucumber Mitochondria DNA fingerprinting is built using the 9 pairs of polymorphism primers for filtering out：
The record of polymorphism mark data：Using the relative position of 0,1 System describe band, clear band is designated as 1, missing note It is 0, according to molecular weight order tape reading from small to large, the band for not having polymorphism refuses statistics；
4. the application of mt DNA fingerprint collection of illustrative plates-cenospecies Purity Identification：
" Nan Shui 3 " is the first generation of hybrid obtained by " Nan Shui 2 " and " one is not weak female " hybridization, " Nan Shui 2 " conduct Female parent, is Mini cucumber, and " one is not weak female ", as male parent, is the cucumber variety from Japan, randomly selects maternal and father This is each 3 plants, extracts DNA, and the amount mixing of material is waited respectively, and 70 plants of the first generation of hybrid extracts DNA, using mitochondrial DNA Polymorphism mark in finger-print carries out Purity Identification, and qualification result is compared with field shape qualification result, 2 kinds of uniformity of authentication method result of checking.
Beneficial effect
Mitochondrial genomes polymorphism of the present invention builds cucumber finger-print and its application, has the advantages that：
1) present invention explores the polymorphism of Cucumber Mitochondria DNA using Cucumber Mitochondria genomic marker；
2) present invention establishes the mt DNA fingerprint collection of illustrative plates of 21 parts of cucumber varieties such as " Chang Chun Mi Ci " first, is cucumber The identification of kind, genetic relationship analysis, the research such as classification and evolution provide a kind of new method, to follow the trail of crossover process Middle paternal mitochondrial genomes do technique preparation, for the application that new varieties " Nan Shui 3 " carry out patent right provides molecule foundation.
3) present invention carries out quick purity mirror using Cucumber Mitochondria genome polymorphism mark to " Nan Shui 3 " seed first It is fixed, to distinguish the false cenospecies of other pollen sources and selfed seed on maternal " Nan Shui 2 " cucumber plant, to formulate " south Water 3 " seedling purity early stage identification technology specification lays the foundation.
4th, illustrate
Fig. 1：21 parts of cucumber variety chondriogen group echo polymorphism screenings such as " Chang Chun Mi Ci ".
1-A：mtSSR10 1-B：mt81 1-C：mt83
In figure, M：Marker, 1：Chang Chun Mi Ci；2：P01(G)；3：Towards excellent No. 3；4：It is flat to hope (G)；5：L8；6：Two early sons；7：Amir；8：8419s； 9：It is American-European good；10：Tree Chun Meiya；11：EC5；12：407 Beit Alpha；13：JCl；14：JC3；15：Boothbyls Blonde； 16：Homenmade Pickles；17：It is peaceful good No. 7；18：Emerald eight；19：Hardwickii；20：One is not weak female；21：Southern water 2
Fig. 2：" Nan Shui 3 " cucumber seeds purity detecting (mtSSR4).
In figure, M：Marker；1：" Nan Shui 2 "；2：" one is not weak female "；3-70：" Nan Shui 3 "
The mitochondrial genomes polymorphism primer of table 1 and its sequence
Table 1 Polymorphism primer information of mitochondrial genome
2 21 parts of cucumber variety mitochondrial DNA digitized battlefield environments of table
Table 2 The mitochondrial DNA digital fingerprint mapping of 21 cucumber varieties
5th, specific embodiment
This implementation using 21 parts of cucumber varieties such as " Chang Chun Mi Ci " as material, using Cucumber Mitochondria genome paternal inheritance characteristic, Cucumber Mitochondria genome polymorphism mark is developed and screened, 21 parts of mitochondrias of cucumber variety such as " Chang Chun Mi Ci " are constructed DNA fingerprinting, and quick Purity is carried out to " Nan Shui 3 " seed using polymorphism mark.Experimentation is as follows：
1. Cucumber Mitochondria genomic marker exploitation：
Compared with promitocondrion genomic data using Cucumber Mitochondria genome weight sequencing data, design Cucumber Mitochondria genome is more State property primer, and the existing Cucumber Mitochondria genome polymorphism SSR primers in Binding experiment room；
2. Cucumber germplasm DNA is extracted and detected：
Cucumber germplasm DNA is extracted with the CTAB methods of improvement, through the μ g mL of 2 μ L 10-1After RNase digestion, with 1% Agarose gel electrophoresis detection DNA mass, -20 DEG C of Refrigerator stores are standby；
3. Cucumber Mitochondria genomic marker polymorphism screening：
1) DNA with 21 parts of cucumber varieties such as " Chang Chun Mi Ci " is template, is entered using following reaction system and PCR programs Row amplification, PCR reaction systems cumulative volume is 24 μ L：The μ L of 2 × Mix 12, sterilize ddH2O 9 μ L, forward and reverse 10 μm of ol L-1 Primer each 1 μ L, template 50ng μ L-1The μ L of DNA 1, are defined as after amplification program optimization：94 DEG C of predegeneration 2min, 94 DEG C Denaturation 20s, 68 DEG C of annealing 1min, 72 DEG C of extension 30s, totally 6 circulate, 94 DEG C of denaturation 20s, 58 DEG C of annealing 1min, 72 DEG C of extension 30s, totally 9 circulations, 94 DEG C of denaturation 20s, 50 DEG C of annealing 30s, 72 DEG C extend 30s, totally 13 circulations, 72 DEG C of extension 5min, 4 DEG C of preservations are entered performing PCR to 21 parts of cucumber samples and are expanded respectively with 81 pairs of mitochondria primers；
2) after PCR amplifications terminate, detect that PCR amplifications are produced using 8% non-denaturing polyacrylamide gel, after 180v electrophoresis 3h Thing, record data, collection electrophoretic image of taking pictures after silver staining colour developing；
4. Cucumber Mitochondria DNA fingerprinting is built using the 9 pairs of polymorphism primers for filtering out：
The record of polymorphism mark data：Using the relative position of 0,1 System describe band, clear band is designated as 1, missing note It is 0, according to molecular weight order tape reading from small to large, the band for not having polymorphism refuses statistics；
5. " Nan Shui 3 " colony checking：
" Nan Shui 3 " is the first generation of hybrid obtained by " Nan Shui 2 " and " one is not weak female " hybridization, " Nan Shui 2 " conduct Female parent, is Mini cucumber, and " one is not weak female ", as male parent, is the cucumber variety from Japan, randomly selects maternal and father This is each 3 plants, extracts DNA, and the amount mixing of material is waited respectively, and 70 plants of the first generation of hybrid extracts DNA, using mitochondrial DNA Polymorphism mark in finger-print carries out Purity Identification, and qualification result is compared with field shape qualification result, 2 kinds of uniformity of authentication method result of checking.

Claims (5)

1. Cucumber Mitochondria genomic marker exploitation：
Compared with promitocondrion genomic data using Cucumber Mitochondria genome weight sequencing data, design Cucumber Mitochondria genome polymorphism primer, and the existing Cucumber Mitochondria genome polymorphism SSR primers in Binding experiment room.
2. the Cucumber Mitochondria genomic marker that pair right 1 is developed carries out polymorphism screening：
1) DNA with 21 parts of cucumber varieties such as " Chang Chun Mi Ci " is template, is expanded using following reaction system and PCR programs, and PCR reaction systems cumulative volume is 24 μ L：The μ L of 2 × Mix 12, sterilize ddH2O 9 μ L, forward and reverse 10 μm of ol L-1Primer each 1 μ L, template 50ng μ L-1The μ L of DNA 1, are defined as after amplification program optimization：94 DEG C of predegenerations 2min, 94 DEG C of denaturation 20s, 68 DEG C of annealing 1min, 72 DEG C of extension 30s, totally 6 circulations, 94 DEG C of denaturation 20s, 58 DEG C of annealing 1min, 72 DEG C of extension 30s, totally 9 circulations, 94 DEG C of denaturation 20s, 50 DEG C of annealing 30s, 72 DEG C of extension 30s, totally 13 circulations, 72 DEG C of extension 5min, 4 DEG C of preservations are entered performing PCR to 21 parts of cucumber samples and are expanded respectively with 81 pairs of mitochondria primers；
2) after PCR amplifications terminate, using 8% non-denaturing polyacrylamide gel, pcr amplification product, record data, collection electrophoretic image of taking pictures after silver staining colour developing are detected after 180v electrophoresis 3h.
3. the 9 pairs of polymorphism primers for being filtered out using right 2 build Cucumber Mitochondria DNA fingerprinting：
The record of polymorphism mark data：Using the relative position of 0,1 System describe band, clear band is designated as 1, and missing is designated as 0, and according to molecular weight order tape reading from small to large, the band for not having polymorphism refuses statistics.
4. the cenospecies Purity Identification of application one of mt DNA fingerprint collection of illustrative plates：
" Nan Shui 3 " is the first generation of hybrid obtained by " Nan Shui 2 " and " one is not weak female " hybridization, " Nan Shui 2 " is used as maternal, it is Mini cucumber, " one is not weak female " is used as male parent, it is the cucumber variety from Japan, randomly select maternal and each 3 plants of male parent, extract DNA, the amount mixing of material is waited respectively, 70 plants of the first generation of hybrid, extracts DNA, and Purity Identification is carried out using the polymorphism mark in the mt DNA fingerprint collection of illustrative plates of right 3, and qualification result is compared with field shape qualification result, verify 2 kinds of uniformity of authentication method result.
5. according to right 1,2,3,4, using Cucumber Mitochondria genome polymorphism, Cucumber Mitochondria DNA fingerprinting is built, fast and effeciently hybridized F1Purity Identification, the method is adapted to whole cucumber varieties.
CN201511003480.8A 2015-12-25 2015-12-25 Mitochondrial genomes polymorphism builds cucumber finger-print and its application Pending CN106916879A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113462803A (en) * 2021-08-02 2021-10-01 青岛农业大学 Cucumber whole genome background selection SNP (Single nucleotide polymorphism) marker system based on multiplex PCR (polymerase chain reaction) sequencing and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102690823A (en) * 2012-06-06 2012-09-26 中国农业科学院蔬菜花卉研究所 SSR (Simple Sequence Repeat) marker tightly interlocked with cucumber fruit bitter gene Bt and application thereof
CN103361425A (en) * 2013-06-13 2013-10-23 广东省农业科学院蔬菜研究所 Primer and method for identifying purity of cucumber 'Yuexiu No.3' hybrid seed
CN104805216A (en) * 2015-05-21 2015-07-29 山东农业大学 Molecular marker for rapidly identifying density of spines of cucumber line, identification method and application
CN105886602A (en) * 2014-12-01 2016-08-24 南京农业大学 Cucumber mitochondrial genome SSR marker development and application of marker in seed purity identification

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102690823A (en) * 2012-06-06 2012-09-26 中国农业科学院蔬菜花卉研究所 SSR (Simple Sequence Repeat) marker tightly interlocked with cucumber fruit bitter gene Bt and application thereof
CN103361425A (en) * 2013-06-13 2013-10-23 广东省农业科学院蔬菜研究所 Primer and method for identifying purity of cucumber 'Yuexiu No.3' hybrid seed
CN105886602A (en) * 2014-12-01 2016-08-24 南京农业大学 Cucumber mitochondrial genome SSR marker development and application of marker in seed purity identification
CN104805216A (en) * 2015-05-21 2015-07-29 山东农业大学 Molecular marker for rapidly identifying density of spines of cucumber line, identification method and application

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
方肇勤: "《分子生物学技术在中医药研究中的应用》", 30 September 2008, 上海科学技术出版社 *
李海梅 等: "黄瓜线粒体基因组SSR标记开发及其在种子纯度鉴定中的应用", 《南京农业大学学报》 *
黄璐琦: "《中药分子鉴定操作指南》", 30 April 2014, 上海科学技术出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113462803A (en) * 2021-08-02 2021-10-01 青岛农业大学 Cucumber whole genome background selection SNP (Single nucleotide polymorphism) marker system based on multiplex PCR (polymerase chain reaction) sequencing and application thereof

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Application publication date: 20170704