CN106914035A - A kind of chromatographic separating process of soluble carbon point system - Google Patents
A kind of chromatographic separating process of soluble carbon point system Download PDFInfo
- Publication number
- CN106914035A CN106914035A CN201511004898.0A CN201511004898A CN106914035A CN 106914035 A CN106914035 A CN 106914035A CN 201511004898 A CN201511004898 A CN 201511004898A CN 106914035 A CN106914035 A CN 106914035A
- Authority
- CN
- China
- Prior art keywords
- carbon point
- solvent
- separating process
- chromatographic separating
- mixed solvent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36 using an external stimulus
- B01D15/3871—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36 using an external stimulus using light
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
A kind of chromatographic separating process of soluble carbon point system, the invention discloses a kind of chromatographic separating process of solubility " carbon point " system.The method is used with silica G as adsorbent, the thin plate of 5 × 20cm, and the medium mixed solvent of selection relative polarity is the thin-layer chromatography of solvent, obtains good separating effect.
Description
Technical field
The present invention relates to a kind of chromatographic separating process of soluble carbon point system, belong to separation technology field.
Technical background
Soluble " carbon point " is the class organic matter similar with coal, charcoal, humus etc. or the incomplete carbon of biomass
The product of change, simply carbonizing degree and the different solubility in water.Comparatively, " carbon point " with
Humus is more similar.The research also main sides of solubility " carbon point " focus on preparation method and application at present, right
Composition and its structure, fluorescence radiation mechanism in system and just show with the research of the interphase interaction of cell tissue
Obtain very weak.Generally " carbon point " be it is nontoxic, harmless be it is unscientific, food barbecuing and smoke
During formed multiring aromatic hydrocarbon " carbon point ", such as 3,4 BaPs be obviously exactly a kind of harmful " carbon
Point ".Food is likely to that " carbon point ", the microblogging that particularly everybody is suspected can be formed during microwave cooking
Cooking method.Different carbon sources, different carbonization methods and condition, the composition of " carbon point " system of formation
And complexity is also differed.Due to the complexity of " carbon point " system, existing " carbon point " system lacks conjunction
Suitable separation method.
The content of the invention
In order to solve above-mentioned the deficiencies in the prior art part, the invention provides a kind of solubility " carbon point " system
Chromatographic separating process.
The purpose of the present invention is realized by following technical scheme：Use with silica G as adsorbent, 5 × 20cm
Thin plate, the medium mixed solvent of selection relative polarity (such as toluene: acetone: dichloromethane=1: 1: 1)
It is solvent, or weak acid (such as glacial acetic acid) is added dropwise on the basis of this mixed solvent, or on this mixed solvent basis
Upper dropwise addition weak base (such as triethylamine), changes the acid-base property of separation system, and adjusts the polarity of mixed solvent.
Brief description of the drawings
Fig. 1 is that toluene-acetone-dichloromethane is schemed for the thin layer of solvent is separated, and wherein a is toluene: acetone:
Dichloromethane=1: the separation situation of 1: 1 mixed solvent；B is toluene: acetone: dichloromethane=1: 1:
1, and the separation situation during drop glacial acetic acid of dropwise addition 3；C is toluene: acetone: dichloromethane=1: 1: 1, and
Separation situation during 3 drop triethylamine is added dropwise.
Specific embodiment
With reference to specific embodiment, the invention will be further described.
1. the preparation of " carbon point " test solution：
4.2g monohydrate potassiums, 2.8g hexas are weighed respectively in 250mL conical flasks, plus
Enter 10mL deionized waters, eddy blending machine concussion is allowed to be completely dissolved.Micro-wave oven heating zone center is put into,
Cooling is taken out after total power microwave certain hour.It is dissolved in water, is transferred to constant volume in 50mL colorimetric cylinders.
The solution after certain volume constant volume is taken in 50mL centrifuge tubes, 10 are centrifuged under the rotating speed of 10000r/min
Min, takes supernatant as sample to be separated.
2. chromatograph：
A. drawn line-transect and frontal line, point sample.Appropriate solvent is added in chromatography cylinder.Chromatography cylinder is inclined
Tiltedly, a little excellent thin plate is put into, and notices that thin plate should not in advance encounter solvent, closed chromatography cylinder.Wait to chromatograph
Its level is put well again after vapo(u)rous in cylinder.Solvent rises to frontal line, takes out thin plate, dries.
B. drawn line-transect and frontal line, point sample.Appropriate solvent is added in chromatography cylinder and three are instilled and drips ice
Acetic acid.Chromatography cylinder is inclined, a little excellent thin plate is put into, and notices that thin plate should not in advance encounter solvent, it is close
Close chromatography cylinder.After its level is put well again after vapo(u)rous in chromatography cylinder.Solvent rises to frontal line, takes out
Thin plate, dries.
C. drawn line-transect and frontal line, point sample.Appropriate solvent is added in chromatography cylinder and three drops three are instilled
Ethamine.Chromatography cylinder is inclined, a little excellent thin plate is put into, and notices that thin plate should not in advance encounter solvent, it is close
Close chromatography cylinder.After its level is put well again after vapo(u)rous in chromatography cylinder.Solvent rises to frontal line, takes out
Thin plate, dries.
3. the observation of fluoroscopic image with record：
Expansion dry after the WFH-203B type dark box type ultraviolet analysis instrument for three purposed that is placed in of chromatography sheet in, 365
Under nm uviol lamps, observed from observation window or shot.
Claims (1)
1. a kind of chromatographic separating process of soluble carbon point system, it is characterized in that using with silica G as adsorbent, 5
The thin plate of × 20cm, the medium mixed solvent of selection relative polarity (such as toluene: acetone: dichloromethane=1:
1: 1), or weak acid (such as glacial acetic acid) is added dropwise on the basis of this mixed solvent, or on the basis of this mixed solvent
It is the thin-layer chromatography of solvent that weak base (such as triethylamine) is added dropwise.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511004898.0A CN106914035A (en) | 2015-12-28 | 2015-12-28 | A kind of chromatographic separating process of soluble carbon point system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511004898.0A CN106914035A (en) | 2015-12-28 | 2015-12-28 | A kind of chromatographic separating process of soluble carbon point system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106914035A true CN106914035A (en) | 2017-07-04 |
Family
ID=59455268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511004898.0A Pending CN106914035A (en) | 2015-12-28 | 2015-12-28 | A kind of chromatographic separating process of soluble carbon point system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106914035A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101954202A (en) * | 2009-07-16 | 2011-01-26 | 中国科学院化学研究所 | Constant-pressure closed ascending chromatographic column device and application method thereof |
| CN102660270A (en) * | 2012-05-03 | 2012-09-12 | 吉林大学 | Method for preparing fluorescent graphene quantum dots by solvothermal method |
| CN102876327A (en) * | 2012-10-29 | 2013-01-16 | 吉林大学 | Method for preparing graphene quantum dots with controllable fluorescence color by chemical modification |
| CN103055827A (en) * | 2013-01-24 | 2013-04-24 | 重庆理工大学 | Chromatographic sheet with self-repairing function and preparation method thereof |
| CN103323587A (en) * | 2013-06-28 | 2013-09-25 | 天津农学院 | Method for detecting imidacloprid by quantum-dot-marked sandwich fluorescence immunoassay |
| CN103497762A (en) * | 2013-06-26 | 2014-01-08 | 上海交通大学 | Method for synthesizing nitrogen doped carbon quantum dot based on one-step monocomponent hydro-thermal synthesis |
| CN103776949A (en) * | 2012-10-18 | 2014-05-07 | 上海原子科兴药业有限公司 | Simple method for examining radiochemical purity of FDG |
| CN104174184A (en) * | 2014-07-31 | 2014-12-03 | 红河学院 | Method for separating chiral drug by using multi-wall carbon nano-tube thin-layer chromatography |
| CN104587990A (en) * | 2015-01-05 | 2015-05-06 | 湖南中医药大学 | Affinity chromatographic medium for carbonyl compound and preparation method of affinity chromatographic medium |
| CN104789217A (en) * | 2015-04-28 | 2015-07-22 | 安徽大学 | Amphipathy carbon quantum dot and preparation method thereof |
| KR20150120664A (en) * | 2014-04-18 | 2015-10-28 | 국립대학법인 울산과학기술대학교 산학협력단 | Carbon dot derivative from sugar alcohols and biological label comprising same |
-
2015
- 2015-12-28 CN CN201511004898.0A patent/CN106914035A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101954202A (en) * | 2009-07-16 | 2011-01-26 | 中国科学院化学研究所 | Constant-pressure closed ascending chromatographic column device and application method thereof |
| CN102660270A (en) * | 2012-05-03 | 2012-09-12 | 吉林大学 | Method for preparing fluorescent graphene quantum dots by solvothermal method |
| CN103776949A (en) * | 2012-10-18 | 2014-05-07 | 上海原子科兴药业有限公司 | Simple method for examining radiochemical purity of FDG |
| CN102876327A (en) * | 2012-10-29 | 2013-01-16 | 吉林大学 | Method for preparing graphene quantum dots with controllable fluorescence color by chemical modification |
| CN103055827A (en) * | 2013-01-24 | 2013-04-24 | 重庆理工大学 | Chromatographic sheet with self-repairing function and preparation method thereof |
| CN103497762A (en) * | 2013-06-26 | 2014-01-08 | 上海交通大学 | Method for synthesizing nitrogen doped carbon quantum dot based on one-step monocomponent hydro-thermal synthesis |
| CN103323587A (en) * | 2013-06-28 | 2013-09-25 | 天津农学院 | Method for detecting imidacloprid by quantum-dot-marked sandwich fluorescence immunoassay |
| KR20150120664A (en) * | 2014-04-18 | 2015-10-28 | 국립대학법인 울산과학기술대학교 산학협력단 | Carbon dot derivative from sugar alcohols and biological label comprising same |
| CN104174184A (en) * | 2014-07-31 | 2014-12-03 | 红河学院 | Method for separating chiral drug by using multi-wall carbon nano-tube thin-layer chromatography |
| CN104587990A (en) * | 2015-01-05 | 2015-05-06 | 湖南中医药大学 | Affinity chromatographic medium for carbonyl compound and preparation method of affinity chromatographic medium |
| CN104789217A (en) * | 2015-04-28 | 2015-07-22 | 安徽大学 | Amphipathy carbon quantum dot and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| ZHAI XINYUN, ET AL: "Highly luminescent carbon nanodots by microwave-assisted pyrolysis", 《CHEMICAL COMMUNICATIONS》 * |
| 杨梅 等: "碳点的制备与柱层析尺寸分离", 《辽宁师范大学学报（自然科学版）》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Insight into the structural features of Zhaotong lignite using multiple techniques | |
| Marcilla et al. | A review of thermochemical conversion of microalgae | |
| Zhang et al. | Effects of pyrolysis temperature and heating time on biochar obtained from the pyrolysis of straw and lignosulfonate | |
| CN103865552B (en) | A kind of method and charcoal preparing charcoal based on agriculture and forestry organic waste material | |
| RU2559491C2 (en) | Method and device for biomass low-temperature pyrolysis | |
| CN103808595B (en) | A kind of asphalt forming process VOC burst size apparatus for collection and analysis | |
| BRPI0417994A (en) | reactor and pyrolysis method | |
| CN111077257B (en) | Method for determining six components of coal tar | |
| Wu et al. | Analysis of wheat straw lignin by thermogravimetry and pyrolysis–gas chromatography/mass spectrometry | |
| CN104215477B (en) | Electron wastes crushes and the harvester of volatile organic matter in Grape berry | |
| Faria et al. | Wildfire‐induced alterations of topsoil organic matter and their recovery in M editerranean eucalypt stands detected with biogeochemical markers | |
| Shimasaki et al. | Detection of age-related fluorescent substances in rat tissues | |
| CN105131984A (en) | Continuous reproduction technology using biomass wastes and waste liquid | |
| CN103408686A (en) | Macroreticular resin capable of lowering content of phenyl hydroxide in cigarette smoke and preparation method thereof | |
| Wang et al. | Changes of sulfur forms in coal after tetrachloroethylene extraction and theirs transformations during pyrolysis | |
| CN106914035A (en) | A kind of chromatographic separating process of soluble carbon point system | |
| CN107651684A (en) | A kind of method that carbonization manufacture activated carbon is pyrolyzed with municipal sludge | |
| CN103076417B (en) | Method for synchronously extracting organic pollutants in water sample | |
| CN105038848B (en) | A kind of preprocess method of biomass pyrolytic tar | |
| Zhao et al. | Microwave-assisted Extraction of Alantolactone and Isoalantolactone from Inula helenium | |
| CN106622137A (en) | Production method of adsorbent for removing mercury from flue gas | |
| CN115974076A (en) | Preparation method and application of hydrothermal nitrogen-doped cork-based activated carbon | |
| CN106693895B (en) | A kind of technique using low-quality charcoal processing lignite upgrading waste water | |
| Yinxin et al. | Preparation and application of biochar from brewerys spent grain and sewage sludge | |
| Nicoara et al. | Development of sample clean up methods for the analysis of Mycobacterium tuberculosis methyl mycocerosate biomarkers in sputum extracts by gas chromatography–mass spectrometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170704 |