CN106908291A - A kind of preparation method of serum glucose standard substance - Google Patents
A kind of preparation method of serum glucose standard substance Download PDFInfo
- Publication number
- CN106908291A CN106908291A CN201710086306.7A CN201710086306A CN106908291A CN 106908291 A CN106908291 A CN 106908291A CN 201710086306 A CN201710086306 A CN 201710086306A CN 106908291 A CN106908291 A CN 106908291A
- Authority
- CN
- China
- Prior art keywords
- serum
- standard substance
- preparation
- glucose
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N2001/2893—Preparing calibration standards
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A kind of preparation method of serum glucose standard substance, it is characterised in that the preparation method is comprised the following steps：(1) preparation of serum：Sources of initial raw materials health donor's blood, after each donor extracts whole blood, obtains serum amount for 110 170 milliliters；(2) treatment of serum：The serum of each donor is mixed, pure glucose substance to the 7mmol/L of aimed concn 5 is added, is centrifuged to realize " the candidate serum standard substance " of homogeneous clarification；Described " candidate serum standard substance " is sub-packed in sterilized dry brown ampere bottle after aseptic filtration, 1 milliliter every bottle, 70 DEG C of frozen for storage；Obtain that intercommunity is good, stable homogeneous, magnitude tracing are reliable, concentration level is 5 7mmol/L human serum extraction standard materials；It has excellent homogeneity, stability and intercommunity, meets the requirement of ISO, is prepared using new blood, wide material sources, the features such as preparation method is simple.
Description
Technical field
The present invention relates to a kind of Glucose standards material with human serum as matrix, a kind of serum grape is particularly related to
The preparation method of Standard for Sugars material.
Background technology
China's diabetes prevalence in increasing sharply trend, by microvascular complication caused by diabetes (PVR,
Diabetic nephropathy, DPN and diabetes) and the incidence of macrovascular complications (Cardial or cerebral vascular diseases), disability rate and
Fatal rate is high, the life quality even life of serious threat diabetic, also causes huge to medical resource and social economy
Big burden, therefore, the concentration of clinical Accurate Determining glucose in serum is early diagnosis and the diabetic of diabetes
Rationally control is key point to blood sugar level.
Standard substance is used for calibration measurement system, evaluation process of measurement or is material assignment.With clinical labororatory's routine
Also corresponding substantial increase is measured the need for the increase of chemical Samples detection amount, standard substance.The approach for preparing of standard substance mainly has：
Artificial synthesized, nature material purification, human or animal tissues source purification product or genetic engineering restructuring standard substance.As matter
Control material, preferably from crude, has similitude as far as possible with sample to be measured, otherwise will be without sufficiently representative
Property.Therefore the human serum matter frost pooled serum standard substance for developing clinical examination is imperative.
The content of the invention
It is an object of the invention to overcome the shortcomings of that prior art is present, and providing one kind has excellent homogeneity, stability
And intercommunity, meet《Primary standard material technical specification》And the requirement of ISO Guide35, prepared using new blood, source
Extensively, the preparation method of the simple serum glucose standard substance of preparation method.
The purpose of the present invention is achieved through the following technical solutions：A kind of preparation side of serum glucose standard substance
Method, it is characterised in that the preparation method is comprised the following steps：
(1) preparation of serum：Sources of initial raw materials health donor's blood, after each donor extracts whole blood, obtains blood
Clear amount is 110-170 milliliters；
(2) treatment of serum：The serum of each donor is mixed, pure glucose substance to aimed concn 5-7mmol/ is added
L, is centrifuged to realize " the candidate serum standard substance " of homogeneous clarification；
Described " candidate serum standard substance " is sub-packed in sterilized dry brown ampere bottle after aseptic filtration, often
1 milliliter of bottle, -70 DEG C of frozen for storage；Obtain that intercommunity is good, stable homogeneous, magnitude tracing are reliable, concentration level is 5-
7mmol/L human serum extraction standard materials.
As preferred：Described healthy donor's selection standard is age 20-50 Sui, blood lipid level is appropriate, human immunity
Defective virus antibody and B-mode, hepatitis C surface antigen negative；Each donor extracts 300-400 milliliters of whole blood, average to obtain
It it is 140 milliliters to serum amount.
As preferred：Described healthy donor draws blood before the meal, is collected in ion dry, without any additive
In bottle, place blood coagulation 3-4 hours at room temperature, be centrifuged 20 minutes under 2000~3000g, serum is isolated from clot.
Glucose initial concentration is determined after serum mixing, pure glucose substance is added to aimed concn, under 6000~9000g
Centrifugation 20 minutes, takes supernatant with the composition such as blood coagulation, fibrin in removal serum sample as far as possible, estimates as clear, yellowish
Color and without particulate matter, UV detector absorbance A under the conditions of wavelength 710nm, 1 centimetre of cuvette is no more than 0.5, if
Do not meet, continue to be centrifuged 20 minutes under 6000~9000g.
As preferred：The described pure glucose substance of addition is the standard substance of numbering GBW10062；Concentration of glucose
Valued methods are gas-chromatography-isotope dilution mass spectrometry.
As preferred：" the candidate serum standard substance " carries out negative-pressure ward filtering in Biohazard Safety Equipment, serum according to
It is secondary to be sterile filtered by the hydrophilic film such as 0.45,0.22 μm.
There is following technique effect relative to prior art in the present invention：The standard substance has good uniformity, stabilization
Property and intercommunity, meet《Primary standard material technical specification》And the requirement of ISO Guide35；Standard substance uses new blood
Prepare, wide material sources, prepare simply, it is easy to use；- 70 DEG C can long term storage, for tracing to the source and matter for conventional inspection systems
Quality assurance during amount is evaluated and cooperating research is analyzed can all play a role, and have great application prospect.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.
A kind of preparation method of serum glucose standard substance of the present invention, the preparation method is comprised the following steps：
(1) preparation of serum：Sources of initial raw materials health donor's blood, after each donor extracts whole blood, obtains blood
Clear amount is 110-170 milliliters；
(2) treatment of serum：The serum of each donor is mixed, pure glucose substance to aimed concn 5-7mmol/ is added
L, is centrifuged to realize " the candidate serum standard substance " of homogeneous clarification；
Described " candidate serum standard substance " is sub-packed in sterilized dry brown ampere bottle after aseptic filtration, often
1 milliliter of bottle, -70 DEG C of frozen for storage；Obtain that intercommunity is good, stable homogeneous, magnitude tracing are reliable, concentration level is 5-
7mmol/L human serum extraction standard materials.
Healthy donor's selection standard of the present invention is age 20-50 Sui, blood lipid level is appropriate, human immune deficiency
Antiviral antibody and B-mode, hepatitis C surface antigen negative；Each donor extracts 300-400 milliliters of whole blood, averagely obtains blood
Clear amount is 140 milliliters.
Healthy donor of the present invention draws blood before the meal, is collected in ion bottle dry, without any additive
In, place blood coagulation 3-4 hours at room temperature, it is centrifuged 20 minutes under 2000~3000g, serum is isolated from clot.
Glucose initial concentration is determined after serum mixing, pure glucose substance is added to aimed concn, under 6000~9000g
Centrifugation 20 minutes, takes supernatant with the composition such as blood coagulation, fibrin in removal serum sample as far as possible, estimates as clear, yellowish
Color and without particulate matter, UV detector absorbance A under the conditions of wavelength 710nm, 1 centimetre of cuvette is no more than 0.5, if
Do not meet, continue to be centrifuged 20 minutes under 6000~9000g.
The described pure glucose substance of addition is the standard substance of numbering GBW10062；The valued methods of concentration of glucose are
Laboratory medicine trace to the source joint committee (JCTLM) accreditation announce one-level reference measure method (gas-chromatography-isotopic dilution matter
Spectrometry), it can be ensured that the traceability of measurement of correlation work.
" candidate serum standard substance " of the present invention carries out negative-pressure ward filtering in Biohazard Safety Equipment, and serum is successively
It is sterile filtered by the hydrophilic film such as 0.45,0.22 μm.
Embodiment：The preparation method of serum glucose standard substance of the present invention is comprised the following steps：
(1) preparation of serum：Sources of initial raw materials health donor's blood.Healthy donor's selection standard is age 20-
50 years old, appropriate blood lipid level, human immune defect virus antibody and B-mode, hepatitis C surface antigen negative, choose 4 taxes altogether
The person of helping, each donor is extracting about 300 milliliters of whole bloods before the meal, is collected in dry ion bottle (without any additive),
Place blood coagulation 3-4 hours at room temperature, be centrifuged 20 minutes under 2000~3000g, serum is isolated from clot；
(2) treatment of serum：The serum of each donor is mixed, about 550 milliliters, determine glucose initial concentration, addition
To aimed concn (5-7mmol/L), the concentration is medical science decision level to pure glucose substance (standard substance GBW10062), it is ensured that
Detection quality, to reduce the rate of missed diagnosis and misdiagnosis rate of diabetes.It is centrifuged under 6000~9000g after pooled serum addition pure material
20 minutes, take supernatant with the composition such as blood coagulation, fibrin in removal serum sample as far as possible, estimate as clear, faint yellow and
Without particulate matter, UV detector absorbance A under the conditions of wavelength 710nm, 1 centimetre of cuvette is no more than 0.5, if not being inconsistent
Conjunction then continues to be centrifuged 20 minutes under 6000~9000g, to realize " the candidate serum standard substance " of homogeneous clarification.In biology
Carry out negative-pressure ward filtering in safety cabinet, serum is sequentially passed through after the hydrophilic film such as 0.45,0.22 μm is sterile filtered, and is sub-packed in
In sterilized dry brown ampere bottle, 1 milliliter every bottle, totally 450 bottles, -70 DEG C of frozen for storage.
(3) uniformity testings：Uniformity is one of important attribute of standard substance, according to《Primary standard material technology is advised
Model》And the requirement of ISO Guide35, the serum reference materials are liquid, belong to the sample having good uniformity, from 450 for preparing
10 bottles are randomly selected in bottle sample, every bottle of replication 3 times carries out uniformity testing.
Serum glucose Certified Reference Material Homogeneity checks measurement data result and statistics to be shown in Table 1~3, in conspicuousness water
Under flat a=0.05, statistical analysis is carried out to data in table 1 using F methods of inspection.
x11, x12... ... x1n1,
x21, x22... x2n2,
... ... ... ... ... ...,
xm1, xm2... ... xmnm,
If
Sum of squares between groups is
Quadratic sum is in group
v1=m-1
v2=N-m
The amount of taking statistics F：
It is (ν according to the free degree1, ν2) and given level of significance α, critical F values can be checked in by table, calculate F values are
1.452, less than Fα(2.393) the interior no significant difference and between group of the serum glucose standard substance group after packing, is illustrated, sample is
Uniformly.
The serum glucose Certified Reference Material Homogeneity of table 1 checks measurement data (unit mmol/L)
Serum glucose Certified Reference Material Homogeneity testing result variance analysis (unit mmol/L) of table 2
The serum glucose Certified Reference Material Homogeneity results of statistical analysis of table 3
3. stability assessment
In order to assess the stability of serum glucose standard substance, the present invention observes the short-term stability of the standard substance
And long-time stability.The stability that short-term stability is mainly tested under the conditions of mock standard matter transportation, long-time stability are then
It is stability of the testing standard material under long-term storage requirement.
Serum glucose standard substance stability assessment measurement data result and statistics are shown in Table 4~5, from average value
Consistency-checking method carries out stability analysis.It is compared with the test result of first time with the test result of different holding times
Analysis.According to formula：
Calculated, whereins1,n1It is average value, the standard of first time standard substance Detection of Stability measurement data
Deviation, pendulous frequency.s2,n2After for separated in time, second standard substance Detection of Stability measurement data it is average
Value, standard deviation, pendulous frequency.
When t and level of significance α, the free degree is n1+n2T inspection critical values t when -2α(n1+n2- 2) relation between is：
t≤tα(n1+n2- 2) when, it is believed that the characteristic magnitude of the standard substance does not occur conspicuousness change, otherwise it is assumed that the standard substance
Characteristic magnitude occurred conspicuousness change.
3.1 short-term stabilities are assessed
Short-term stability experiment is concerned with the stability of the standard substance under specific traffic condition.According to current China
Traffic condition, using express transportation, goods can be transported to China main cities in 2 days.The design of this experiment is as follows：Simulation mark
The environment of quasi- material hair sample and transportation, analyzes the change situation of concentration of glucose in the process.Specifically include following several
Individual step：Would be stored at -70 DEG C of environment Plays materials first to take out rapidly, the interior outsourcing before being transported under the dry-ice temperature
Dress.It is the foam box of 1.8cm that external packing uses wall thickness, and dry ice 1kg is added in each foam box.
The foam box that will be equipped with standard substance candidate and dry ice is placed in room temperature (25 DEG C) environment, in the 0h of storage,
9h, 24h, 33h, 48h, 57h, 72h are sampled to it, and -70 DEG C of refrigerators are placed in after sampling.Observation caliber material before per sub-sampling
State, be changed into after liquid stopping sampling detection.After gross sample is finished, examination of glucose concentration is carried out to sample.
Serum glucose standard substance short-term stability assessment of the measurement result (unit mmol/L) of table 4
3.2 long-time stability are assessed
Long-time stability experiment is concerned with the stability of the standard substance concentration of glucose under specific storage requirement.
This experiment is that under -70 DEG C of storage requirements, the standard substance to storing is sampled analysis.
Serum glucose standard substance long-time stability assessment of the measurement result (unit mmol/L) of table 5
4. definite value
4.1 gas chromatograph-mass spectrometer GC-MS (Agilent 7890-5975C) parameter setting
Chromatographic column：Agilent DB-5 capillary columns；Carrier gas is high-purity helium, and flow velocity is 1.0mL/min；Input mode is
Shunting, sample size is 1 μ L, and split ratio is 10:1,270 DEG C of injector temperature；Column temperature is warmed up to from 80 DEG C with 20 DEG C/min speed
280 DEG C, keep 10min；Ion source temperature：230℃；Acquisition mode：Scan and SIM (Salbutamol Selected Ion Monitoring), selection detection from
Son：M/z 314 and m/z 319.
4.2 sample derivation process
It is accurate weigh glucose sterlings standard substance (GBW10062) of D mono-, D- [I3C6] glucose marker thing (ALDRICH)
It is dissolved in pure water；Accurate measuring standard substance, the glucose sterling titers of D mono- (about 100 μ l), add a certain amount of glucose mark
Note thing solution, jog is fully mixed, and is balanced 2 hours in 4 DEG C of refrigerators；To being added in the sample for having added label, 0.8mL is anhydrous
Ethanol, vibration is mixed, and deproteinized is removed with 5000r/min centrifugations 10min at 4 DEG C, takes supernatant liquor, nitrogen drying at 60 DEG C；
The pyridine solution (0.2mol/L) of 150 μ L hydroxylamine hydrochlorides is added, is mixed, reacted 0.5 hour at 90 DEG C；After cooling, 200 are added
μ L acetic anhydrides, mix, and are reacted 0.5 hour at 90 DEG C；Nitrogen drying at 60 DEG C, adds 500 μ L dichloromethane to redissolve, and crossing has machine filter
Film, carries out the detection of GC/ID-MS.
Concentration of glucose result of calculation in 4.3 samples
According to the peak area ratio of glucosan derivative mass ions m/z 314 and m/z 319, using internal standard single-point method meter
Calculate, the results are shown in Table 6.
Serum glucose standard substance value data (unit mmol/L) of table 6
5. uncertainty evaluation
According to Guide 35《Definite value-the generic principles and Statistics of standard substance/standard sample》Requirement, use
The principle and method of GUM and QUAM quantify to the partial uncertainty of standard substance characteristic value.
The uncertainty source of characteristic value mainly has：1) uncertainty of sterling standard substance；2) characteristic value measurement process
The uncertainty for causing；3) uncertainty that difference causes between bottle；4) uncertainty that stability causes.
The calculating of 5.1 standard uncertainties
1) the standard uncertainty u of sterling standard substance GBW100621：
GBW10062 glucose definite value be 99.6%, expanded uncertainty be 0.5% (k=2), therefore its contribution it is relative
Standard uncertainty is：0.5% ÷ 2=0.25%.
2) the standard uncertainty u that measurement process is introduced2：
The standard uncertainty that measurement process is introduced is the standard deviation S D of measurement result, and its relative standard uncertainty is
Measurement relative variability coefficient CV=0.42%
3) the standard uncertainty u that difference causes between bottle3：
The uniformity data that standard substance is waited carries out one-way analysis of variance and is shown in Table 2, and inhomogeneities is introduced between assessing its bottle
Standard uncertainty.
The relative standard that i.e. uniformity causes does not know to be 0.34%.
4) the uncertainty u that stability causes4：
Standard substance candidate long-time stability data are shown in Table 5.From linear equation as standard substance empirical model,
Fitting a straight line and to calculate the uncertainty related to slope be the uncertainty that its long-time stability causes, the results are shown in Table 7：
The uncertainty evaluation that the long-time stability of table 7 cause
The calculating of 5.2 Composite Seismograms
Combined standard uncertainty is calculated according to following equation：
Combined standard uncertainty=5.84mmol/L × the 0.686%=of serum glucose standard substance definite value
0.04mmol/L。
Expanded uncertainty Coverage factor k=2, U=k × Uc=2 × 0.04=0.08mmol/L.
Standard substance prepared by the present invention：Glucose sign value is 5.84mmol/L, and expanded uncertainty is 0.08mmol/
L (k=2).
Claims (5)
1. a kind of preparation method of serum glucose standard substance, it is characterised in that the preparation method is comprised the following steps：
(1) preparation of serum：Sources of initial raw materials health donor's blood, after each donor extracts whole blood, obtains serum amount
It is 110-170 milliliters；
(2) treatment of serum：The serum of each donor is mixed, pure glucose substance to aimed concn 5-7mmol/L is added, from
The heart with realize it is homogeneous, clarification " candidate serum standard substance "；
Described " candidate serum standard substance " is sub-packed in sterilized dry brown ampere bottle after aseptic filtration, every bottle 1
Milliliter, -70 DEG C of frozen for storage；Obtain that intercommunity is good, stable homogeneous, magnitude tracing are reliable, concentration level is 5-7mmol/L
Human serum extraction standard material.
2. the preparation method of serum glucose standard substance according to claim 1, it is characterised in that described health tax
The person's of helping selection standard is age 20-50 Sui, appropriate blood lipid level, human immune defect virus antibody and B-mode, hepatitis C table
Face antigen negative；Each donor extracts 300-400 milliliters of whole blood, averagely obtains serum amount for 140 milliliters.
3. the preparation method of serum glucose standard substance according to claim 1 and 2, it is characterised in that described health
Donor draws blood before the meal, is collected in ion bottle dry, without any additive, places blood coagulation 3-4 at room temperature small
When, it is centrifuged 20 minutes under 2000~3000g, serum is isolated from clot；
Glucose initial concentration is determined after serum mixing, pure glucose substance is added to aimed concn, is centrifuged under 6000~9000g
20 minutes, take supernatant with the composition such as blood coagulation, fibrin in removal serum sample as far as possible, estimate as clear, faint yellow and
Without particulate matter, UV detector absorbance A under the conditions of wavelength 710nm, 1 centimetre of cuvette is no more than 0.5, if not being inconsistent
Conjunction then continues to be centrifuged 20 minutes under 6000~9000g.
4. serum glucose standard substance preparation method according to claim 3, it is characterised in that the pure Portugal of described addition
Grape sugar substance is the standard substance of numbering GBW10062；The valued methods of concentration of glucose are gas-chromatography-isotopic dilution matter
Spectrometry.
5. serum glucose standard substance preparation method according to claim 1, it is characterised in that described " candidate serum
Standard substance " carries out negative-pressure ward filtering in Biohazard Safety Equipment, and serum sequentially passes through the hydrophilic film such as 0.45,0.22 μm and carries out nothing
Bacterium is filtered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710086306.7A CN106908291A (en) | 2017-02-17 | 2017-02-17 | A kind of preparation method of serum glucose standard substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710086306.7A CN106908291A (en) | 2017-02-17 | 2017-02-17 | A kind of preparation method of serum glucose standard substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106908291A true CN106908291A (en) | 2017-06-30 |
Family
ID=59207729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710086306.7A Pending CN106908291A (en) | 2017-02-17 | 2017-02-17 | A kind of preparation method of serum glucose standard substance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106908291A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107505460A (en) * | 2017-08-07 | 2017-12-22 | 嘉兴博泰生物科技发展有限公司 | C reactive protein quality for POCT controls the preparation method of product |
| CN110849830A (en) * | 2019-11-15 | 2020-02-28 | 首都医科大学附属北京朝阳医院 | Serum copper standard substance and preparation method and application thereof |
| CN112986065A (en) * | 2021-02-08 | 2021-06-18 | 杭州同创医学检验实验室有限公司 | Whole blood quality control product for hematology analyzer and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1159629A (en) * | 1967-02-13 | 1969-07-30 | Baxter Laboratories Inc | Analytical Reference Standard |
| JPS6134455A (en) * | 1984-07-26 | 1986-02-18 | Shimadzu Corp | Standard liquid for blood sample analysis |
| TW200835914A (en) * | 2006-10-26 | 2008-09-01 | Bionostics Inc | Novel standard reference solutions |
| CN101344528A (en) * | 2008-08-27 | 2009-01-14 | 中生北控生物科技股份有限公司 | Bovine serum cholesterol standard substance and use thereof |
| CN101701962A (en) * | 2009-11-19 | 2010-05-05 | 首都医科大学附属北京朝阳医院 | Serum magnesium standard substance |
| CN101930009A (en) * | 2009-11-19 | 2010-12-29 | 首都医科大学附属北京朝阳医院 | Serum calcium standard substance |
| CN105092336A (en) * | 2015-08-28 | 2015-11-25 | 宁波瑞源生物科技有限公司 | Preparation method of stable glycated albumin calibrating material and quality control material |
-
2017
- 2017-02-17 CN CN201710086306.7A patent/CN106908291A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1159629A (en) * | 1967-02-13 | 1969-07-30 | Baxter Laboratories Inc | Analytical Reference Standard |
| JPS6134455A (en) * | 1984-07-26 | 1986-02-18 | Shimadzu Corp | Standard liquid for blood sample analysis |
| TW200835914A (en) * | 2006-10-26 | 2008-09-01 | Bionostics Inc | Novel standard reference solutions |
| CN101344528A (en) * | 2008-08-27 | 2009-01-14 | 中生北控生物科技股份有限公司 | Bovine serum cholesterol standard substance and use thereof |
| CN101701962A (en) * | 2009-11-19 | 2010-05-05 | 首都医科大学附属北京朝阳医院 | Serum magnesium standard substance |
| CN101930009A (en) * | 2009-11-19 | 2010-12-29 | 首都医科大学附属北京朝阳医院 | Serum calcium standard substance |
| CN105092336A (en) * | 2015-08-28 | 2015-11-25 | 宁波瑞源生物科技有限公司 | Preparation method of stable glycated albumin calibrating material and quality control material |
Non-Patent Citations (2)
| Title |
|---|
| 刘欧等: "5水平冰冻混合人血清葡萄糖候选标准物质的制备研究", 《中国实验诊断学》 * |
| 申玉星等: "液相色谱-同位素稀释质谱法准确测定人血清中葡萄糖含量", 《质谱学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107505460A (en) * | 2017-08-07 | 2017-12-22 | 嘉兴博泰生物科技发展有限公司 | C reactive protein quality for POCT controls the preparation method of product |
| CN110849830A (en) * | 2019-11-15 | 2020-02-28 | 首都医科大学附属北京朝阳医院 | Serum copper standard substance and preparation method and application thereof |
| CN112986065A (en) * | 2021-02-08 | 2021-06-18 | 杭州同创医学检验实验室有限公司 | Whole blood quality control product for hematology analyzer and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mohun et al. | Simple methods for measuring serum levels of the glutamic-oxalacetic and glutamic-pyruvic transaminases in routine laboratories | |
| Neeld Jr et al. | Macro-and micromethods for the determination of serum vitamin A using trifluoroacetic acid | |
| Cheng et al. | Comparison of pleural fluid pH values obtained using blood gas machine, pH meter, and pH indicator strip | |
| CN104808006B (en) | A kind of glycosylated hemoglobin standard substance and preparation method thereof | |
| CN102091283B (en) | Method for detecting Xingnaojing injection | |
| CN106908291A (en) | A kind of preparation method of serum glucose standard substance | |
| CN108303548A (en) | A kind of calibrating method improving c reactive protein testing result consistency | |
| ITUD20080190A1 (en) | PROCEDURE FOR THE BACTERIOLOGICAL SURVEY ON PLASMA | |
| CN101344528B (en) | Bovine serum cholesterol standard substance and use thereof | |
| CN107586776A (en) | Suitable for the plasmid control material of H7N9 avian influenza virus real-time fluorescence quantitative PCR detection | |
| CN106226425A (en) | Serum glycated albumin detection method and special candidate criteria material thereof | |
| CN102519925A (en) | Kit for screening and checking glucose-6-phosphate dehydrogenase (G6PD) deficiency of neonates and preparation method for kit | |
| CN104181313A (en) | Preparation method of blood coagulation factor IX quality control product | |
| Cao et al. | Determination of clinically acceptable cut-offs for hemolysis index: An application of bootstrap method using real-world data | |
| CN103940783A (en) | Method for predicting shelf-life stability of sterilized milk | |
| CN106124682A (en) | A kind of composition method of inspection of Radix Et Caulis Acanthopanacis Senticosi injection | |
| CN102023221B (en) | Quality control experimental method for full-automatic platelet aggregation instrument | |
| Kim et al. | Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases | |
| CN106168610A (en) | Fluorimetric HPLC measures the method for concentration of Clozapine in blood plasma | |
| CN109239374A (en) | A kind of liquid thrombin time detection reagent | |
| CN114137107A (en) | Method for measuring content of deoxycholic acid injection | |
| CN109097453A (en) | A kind of RNA nucleic acid definite value Quality Control object and preparation method thereof | |
| CN107505460A (en) | C reactive protein quality for POCT controls the preparation method of product | |
| CN109254146A (en) | TORCH-IgM antibody mixing quality-control product | |
| Spezia et al. | Anticoagulant Choices Affect the Mean Platelet Volume Measurement by Impedance. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170630 |