CN106906266A - A kind of utilization alpha amylase prepares the method and Fructus Hordei Germinatus oligose alcohol of Fructus Hordei Germinatus oligose alcohol - Google Patents
A kind of utilization alpha amylase prepares the method and Fructus Hordei Germinatus oligose alcohol of Fructus Hordei Germinatus oligose alcohol Download PDFInfo
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Abstract
The present invention provides a kind of method that utilization alpha amylase prepares Fructus Hordei Germinatus oligose alcohol, and it includes：By starch dispersion in 30~60% alcoholic solution, pretreatment of starch thing is dried to obtain after being processed through ultramicro grinding.Pretreatment of starch thing is configured to 55~65% starch milk, thermostable α-amylase is added, liquefaction obtains liquefied solution.Middle temperature alpha amylase, beta amylase and Pullulanase are added in liquefied solution, after ultrasonically treated 8~10min under conditions of 20~30W, saccharification obtains saccharified solution.Fructus Hordei Germinatus oligose alcohol is obtained after saccharified solution hydrogenation.The present invention also improves a kind of Fructus Hordei Germinatus oligose alcohol, is prepared according to the above method.The above method is simple, the control of parameters solution, and enzymatic activity is high in preparation process, and enzyme dosage is few, and using the starch milk of high concentration, production efficiency is high, and low production cost, obtained Fructus Hordei Germinatus oligose alcohol is best in quality.
Description
Technical field
The present invention relates to enzyme engineering field, and more particularly to a kind of utilization AMS prepare Fructus Hordei Germinatus oligose alcohol method and
Fructus Hordei Germinatus oligose alcohol.
Background technology
Fructus Hordei Germinatus oligose alcohol be Fructus Hordei Germinatus oligose hydrogenation after formed sugar alcohol, be after D-sorbite, mannitol, antierythrite,
The important novel sugar alcohol that newly-developed gets up after maltitol, xylitol, arabite etc., be food, daily use chemicals, medicine,
Epochmaking supplementary material in the fields such as agricultural.The excellent in physical and chemical performance of Fructus Hordei Germinatus oligose alcohol, its sugariness is low, heat-resist, no
Generation Maillard reaction, anti-crystallization are strong, water holding moisture retention is good, permeability is suitable, and the harmonicity with other sweeteners is good, tool
There is absorption and retain the function of effumability fragrance and flavor substance.Additionally, the energy of Fructus Hordei Germinatus oligose alcohol is low, blood sugar will not be caused
Fluctuation, with physiological functions such as non-cariogenic tooth, digestible degree reductions, makes it in sugar-free candy, low-calorie diet and diabetes
The fields such as special food have very important effect.
With the development of science and technology with the raising of human health demand, the market demand of Fructus Hordei Germinatus oligose alcohol can be increasingly
Greatly, the research application for preparing maltitol using starchiness resource become very urgently with it is important.
The content of the invention
It is an object of the invention to provide a kind of method that utilization AMS prepares Fructus Hordei Germinatus oligose alcohol, the letter of the method technique
Single, production is efficient, it is adaptable to industrialization large-scale production.
Another object of the present invention is to provide a kind of Fructus Hordei Germinatus oligose alcohol, this Fructus Hordei Germinatus oligose alcohol with starch material, by α-
The enzymolysis such as amylase obtain Fructus Hordei Germinatus oligose alcohol best in quality.
The present invention is solved its technical problem and is realized using following technical scheme.
The present invention proposes a kind of method that utilization AMS prepares Fructus Hordei Germinatus oligose alcohol, and it includes：：
Pretreatment：By starch dispersion in the alcoholic solution that volume fraction is 30~60%, dried after being processed through ultramicro grinding
Obtain pretreatment of starch thing.
Liquefaction reaction：Pretreatment of starch thing is configured to the starch milk that mass fraction is 55~65%, add high temperature resistant α-
Amylase, liquefied solution is obtained through liquefaction reaction.
Saccharification reaction：Complex enzyme is added in liquefied solution, after ultrasonically treated 8~10min under conditions of 20~30W,
Through being saccharified, reaction obtains saccharified solution, wherein, complex enzyme includes mesophilicα-diastase, beta amylase and Pullulanase.
Hydrogenation reaction：Saccharified solution obtains Fructus Hordei Germinatus oligose alcohol by hydrogenation reaction.
The present invention proposes a kind of Fructus Hordei Germinatus oligose alcohol, and it is prepared according to the above method.
Compared with prior art, beneficial effects of the present invention are：
The method that utilization AMS of the invention prepares Fructus Hordei Germinatus oligose alcohol is simple, and parameters are easily controlled, obtained
Fructus Hordei Germinatus oligose alcohol it is best in quality.Pretreatment of starch thing is obtained by soybean dietary fiber, the solubility of starch is greatly improved
And enzymolysis activity, can be liquefied using high-concentration starch milk, and starch enzyme dosage and enzymolysis time can be saved, improve life
Produce efficiency.After starch milk is processed through Thermostable α-Amylase, the liquefied solution with certain DE values is obtained.Liquefied solution is in middle temperature
The saccharified solution of Fructus Hordei Germinatus oligose content high is obtained under the complex enzyme hydrolysis effect of AMS, beta amylase and Pullulanase.It is many
The synergy of enzyme is planted, without using enzyme preparations such as costly maltotriose enzymes.Additionally, being digested using complex enzyme
When, advance with the liquefied solution that ultrasonication adds complex enzyme.Ul-trasonic irradiation on the one hand cause liquefied solution in
Grain shows to rupture, is recessed, and improves the reactivity of liquefied solution.On the other hand, low frequency ultrasound treatment complex enzyme, energy
Enough effectively improve the enzyme activity of complex enzyme so that the enzyme digestion reaction process efficiency of complex enzyme is higher, effectively reduces enzyme dosage, improve
Production efficiency.
Specific embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below will be in the embodiment of the present invention
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional product that can be obtained by commercially available purchase
Product.
The method and Fructus Hordei Germinatus oligose that prepare Fructus Hordei Germinatus oligose alcohol using AMS to the embodiment of the present invention have below
Body explanation.
First, pre-treatment step：By starch dispersion in the alcoholic solution that volume fraction is 30~60%, at ultramicro grinding
Pretreatment of starch thing is dried to obtain after reason.
Native starch particles are a kind of polycrystalline structure compounds, and it is internal with crystal region and noncrystalline domain.Crystal region master
If being formed with double-spiral structure by branched amylopectin molecules, structure is comparatively dense, is difficult to be acted on by external force and chemical reagent, crystallization
The presence in area, limits the reactivity of starch to a certain extent.Noncrystalline domain is mainly by amylose molecule with loose knot
It is configured to, is easily acted on by external force and chemical reagent.It is understood that the starch in the embodiment of the present invention can form sediment selected from corn
One or more in powder, wheaten starch, tapioca, but it is not limited to this.
Alcoholic solution is the aqueous solution of alcohol, and alcoholic solution can effectively suppress the expansion characteristics of starch granules, the dehydration of alcohol,
Can suppress starch granules absorb excessive moisture and so that starch granules excessively expands.It is excellent by starch dispersion in alcoholic solution
Selection of land, is scattered in ethanol solution, and then by soybean dietary fiber, the mechanical force of high speed shear causes that starch granules occurs to break
Split, be denatured, the birefringent phenomenon of starch is disappeared, and crystalline texture is destroyed.
In the production process of existing glycitols product, typically mass fraction is used to enter for 15~20% or so starch milk
Row treatment, low production efficiency.And in the embodiment of the present invention, by the starch for pre-processing, the crystalline texture of starch is destroyed, water
Easily penetrate into inside starch molecule, the solubility and dilation of starch are increasing, meanwhile, in the presence of mechanical force, form sediment
The good dispersion of powder, water imbibition are strong, specific surface area is big, chemistry and biological respinse activity are high.Therefore, it is possible to utilize high concentration (quality
Fraction is that 55~starch milk 65%) is processed, and needs not move through insulation cooking step, and production efficiency is greatly carried
It is high.What is more important, by the starch for pre-processing, amylase is easily advanced into inside particle, and enzymolysis activity is obtained greatly
Improve, it is only necessary to which a small amount of amylase and shorter enzymolysis time can just reach good liquefaction effect, save biological enzyme dosage,
Reduce production cost.
Further, in present pre-ferred embodiments, ultramicro grinding is processed as the first homogeneous under 10~15MPa pressure,
Carry out microjet homogenization under conditions of 100~130MPa of pressure, 50~60 DEG C of temperature again, obtain average grain diameter for 20~
80 μm of pretreatment of starch thing.The homogenizing process enables to starch granules particle diameter effectively to lower, and the crystalline texture of starch is subject to
Effectively destruction, the reactivity and enzymolysis activity of starch are optimal level.
Secondly, pretreatment of starch thing is configured to the starch milk that mass fraction is 55~65%, adds high temperature resistant alphalise starch
Enzyme, liquefied solution is obtained through liquefaction reaction.
Preferably, inventor prepares Thermostable α-Amylase (α-amylase, EC using biological engineering method
3.2.1.1), its gene comes from bacillus licheniformis (Bacillus licheniformis).
Further, in present pre-ferred embodiments, after adding Thermostable α-Amylase in starch milk, in 70~80W
Under the conditions of carry out liquefaction reaction again after ultrasonically treated 2~3min.Carried out under above-mentioned condition it is ultrasonically treated, can cause high temperature resistant α-
The enzymatic activity of amylase effectively improves, and causes that starch milk reactivity is higher.
Further, in present pre-ferred embodiments, temperature be 95~105 DEG C, pH be 6.5~7.5 under the conditions of liquid
Change 4~6min of reaction, the consumption of Thermostable α-Amylase is preferably 30~45U/g pretreatment of starch things.By investigating, above-mentioned
Under reaction conditions, liquefied solution of the DE values 15~25% can be obtained, the liquefied solution in the range of the DE values is through follow-up
In the saccharified solution that treatment is obtained, the content of Fructus Hordei Germinatus oligose is higher.
Again, complex enzyme is added in liquefied solution, after ultrasonically treated 8~10min under conditions of 20~30W, through sugar
Change reaction and obtain saccharified solution, wherein, complex enzyme includes mesophilicα-diastase, beta amylase and Pullulanase.
AMS can cut α -1,4 glycosidic bonds from starch molecule internal random；Beta amylase can be from starch chain
Non-reducing end start, sequentially cut maltose unit；Pullulanase can selectivity cut α -1,6 in amylose branch
Glycosidic bond.Liquefied solution is saccharified by the different enzymolysis of three kinds of enzymes, obtains the saccharified solution containing Fructus Hordei Germinatus oligose.
The ultrasonication of lower-wattage is used before saccharification for a period of time.Mechanism of Ultrasonic is cavitation, heating
Effect and high frequency oscillation effect, are on the one hand ul-trasonic irradiations in liquefied solution under ul-trasonic irradiation, are produced in a liquid empty
Change generation, growth, the collapse of core, so as to produce local high temperature, high pressure and dither, the particle in liquefied solution shows
Rupture, depression etc., reactivity is higher.On the other hand, in complex enzyme, enzymatic activity can be given birth to influences ul-trasonic irradiation.Ultrasound
The factors such as power, the time for the treatment of are produced for enzymatic activity and promoted or inhibitory action.Under the ultrasound condition of above-mentioned parameter, compared with
A period of time is processed under low power, enzyme molecule can be transferred energy to, and the structure of enzyme molecule will not damaged, made
Enzymatic activity is obtained to effectively improve.Particularly if by complex enzyme immobilization, enzyme and substrate can be in different phases, and ultrasound is very
The structural form of material molecule can be made to change in big degree, increase permeability, accelerate the reaction of substrate and enzyme molecule, from
And cause that enzyme activity is improved.
Further, in present pre-ferred embodiments, the amount ratio of mesophilicα-diastase, beta amylase and Pullulanase
It is 1:5~7:0.5~2.Under above-mentioned enzyme ratio, the synergy of each enzyme reaches most preferably, the product wheat obtained after saccharification
Bud oligosaccharide content is optimal.
Further, in present pre-ferred embodiments, temperature be 50~60 DEG C, pH be 5~6 under the conditions of be saccharified reaction
5~7h, the consumption of mesophilicα-diastase is preferably 10~15U/g pretreatment of starch things.Due to the optimum reaction condition of different enzymes
Difference, during compound use, the amount ratio of the various enzymes of integrated survey and the reaction condition of various enzymes are preferably obtained above-mentioned
Under reaction condition, under the conditions of being somebody's turn to do, the overall activity of complex enzyme is optimal, to greatest extent using substrate, obtains Fructus Hordei Germinatus oligose content high
Product.
Further, in present pre-ferred embodiments, for the ease of the recovery of enzyme preparation, recycling, by complex enzyme
It is fixed on carrier, carrier is prepared as follows and obtains：By silicon source and surfactant in molar ratio 1:0.15~0.3 dissolving
In acid solution or alkaline solution, 2~3h of stirring reaction obtains the first solution.Then by resorcinol and hexamethylenetetramine
In molar ratio 5:1~2 is dissolved in solvent, obtains the second solution.Then by the first solution and the second solution in mass ratio 2~4:
1 mixing, and polymerization form polymer, by after polymeric seal under the conditions of 120~150 DEG C 4~8h of solvent heat treatment, dry after
Obtain precursor powder.Finally precursor powder is reacted in carbon dioxide atmosphere, reaction temperature is 600~680 DEG C, reaction
Time is 3~6h, obtains carrier.Preferably, silicon source selects the butyl ester of positive silicic acid four (TEOS), and surfactant selects cetyl
Ammonium bromide (CTAB).
Further, in present pre-ferred embodiments, complex enzyme is with the fixing means of carrier：Complex enzyme is scattered in
In water, carrier, 25~35min of concussion reaction under the conditions of 20~30 DEG C are added.The carrier prepared by the above method,
In the presence of carbon dioxide, charing, activation and the mesoporous silicon oxide for realizing polymer burn till, process is simple, and
The carrier for obtaining is the mixing material of activated carbon and silica, and specific surface area is big, can just be adsorbed well without crosslinking agent
Enzyme molecule, and carrier has high mesoporous rate, can efficient loading enzyme molecule.
Finally, saccharified solution obtains Fructus Hordei Germinatus oligose alcohol by hydrogenation reaction.
Further, in present pre-ferred embodiments, be saccharified reaction solution in add mass fraction be 0.01~
0.03% potassium borohydride and mass fraction are 2~3% catalyst, are carried out under the conditions of 8~10MPa, 120~135 DEG C
Hydrogenation reaction.Preferably, from Raney nickel.In hydrogenation process, the addition of potassium borohydride can be hydrogenated in residual sugar
Aldehyde radical, shortens the hydrogenation time, reducing energy consumption, improves hydrogenation yield.
It should be noted that before carrying out hydrogenation reaction or after hydrogenation reaction step, being filtered with reactant, it is pure
The operation such as change, refined, further to improve the purity of Fructus Hordei Germinatus oligose alcohol.
The embodiment of the present invention also provides a kind of preparation method of Fructus Hordei Germinatus oligose alcohol, and it is prepared according to the above method.
Feature of the invention and performance are described in further detail with reference to embodiments.
Embodiment 1
The method that a kind of utilization AMS that the present embodiment is provided prepares Fructus Hordei Germinatus oligose alcohol, it includes：
Pretreatment：By starch dissolution in the ethanol solution that volume fraction is 50%, starch is dried to obtain after ultramicro grinding
Pretreatment thing.
Liquefaction：Pretreatment of starch thing is added water the starch milk for being made into that mass fraction is 60%, add 40U/g high temperature resistant α-
Amylase, after ultrasound 2min under the conditions of 80W, reaction 5min obtains liquefied solution under the conditions of 100 DEG C, pH are for 7.
Saccharification：The mesophilicα-diastase of addition 10U/g, the beta amylase of 60U/g and 20U/g's is general in liquefied solution
Shandong orchid enzyme, after ultrasound 9min under the conditions of 25W, reaction 6h obtains saccharified solution under the conditions of 55 DEG C, pH are for 5.5.
Hydrogenation：Saccharified solution is placed in hydrogenation kettle, potassium borohydride and mass fraction that mass fraction is 0.02% is added
It is 2% Raney nickel, 2.5h is reacted under the conditions of 10MPa, 130 DEG C and obtains Fructus Hordei Germinatus oligose alcohol.
Embodiment 2
The method that a kind of utilization AMS that the present embodiment is provided prepares Fructus Hordei Germinatus oligose alcohol, it includes：
Pretreatment：By starch dissolution in the ethanol solution that volume fraction is 30%, starch is dried to obtain after ultramicro grinding
Pretreatment thing.
Liquefaction：Pretreatment of starch thing is added water the starch milk for being made into that mass fraction is 55%, add 30U/g high temperature resistant α-
Amylase, after ultrasound 2min under the conditions of 80W, reaction 6min obtains liquefied solution under the conditions of 95 DEG C, pH are for 6.5.
Saccharification：The mesophilicα-diastase of addition 10U/g, the beta amylase of 70U/g and 5U/g's is general in liquefied solution
Shandong orchid enzyme, after ultrasound 8min under the conditions of 30W, reaction 7h obtains saccharified solution under the conditions of 50 DEG C, pH are for 6.
Hydrogenation：Saccharified solution is placed in hydrogenation kettle, potassium borohydride and mass fraction that mass fraction is 0.03% is added
It is 3% Raney nickel, 2h is reacted under the conditions of 8MPa, 135 DEG C and obtains Fructus Hordei Germinatus oligose alcohol.
Embodiment 3
The method that a kind of utilization AMS that the present embodiment is provided prepares Fructus Hordei Germinatus oligose alcohol, it includes：
Pretreatment：By starch dissolution in the ethanol solution that volume fraction is 60%, starch is dried to obtain after ultramicro grinding
Pretreatment thing.
Liquefaction：Pretreatment of starch thing is added water the starch milk for being made into that mass fraction is 65%, add 45U/g high temperature resistant α-
Amylase, after ultrasound 3min under the conditions of 70W, reaction 4min obtains liquefied solution under the conditions of 105 DEG C, pH are for 7.5.
Saccharification：The mesophilicα-diastase of addition 15U/g, the beta amylase of 65U/g and 15U/g's is general in liquefied solution
Shandong orchid enzyme, after ultrasound 10min under the conditions of 20W, reaction 5h obtains saccharified solution under the conditions of 60 DEG C, pH are for 5.
Hydrogenation：Saccharified solution is placed in hydrogenation kettle, potassium borohydride and mass fraction that mass fraction is 0.03% is added
It is 3% Raney nickel, 2h is reacted under the conditions of 10MPa, 120 DEG C and obtains Fructus Hordei Germinatus oligose alcohol.
Embodiment 4
The method that a kind of utilization AMS that the present embodiment is provided prepares Fructus Hordei Germinatus oligose alcohol, it includes：
Pretreatment：By starch dissolution in the ethanol solution that volume fraction is 50%, starch is dried to obtain after ultramicro grinding
Pretreatment thing.
Liquefaction：Pretreatment of starch thing is added water the starch milk for being made into that mass fraction is 60%, add 40U/g high temperature resistant α-
Amylase, after ultrasound 2min under the conditions of 80W, reaction 5min obtains liquefied solution under the conditions of 100 DEG C, pH are for 7.
Saccharification：The mesophilicα-diastase of addition 12U/g, the beta amylase of 60U/g and 18U/g's is general in liquefied solution
Shandong orchid enzyme, after ultrasound 9min under the conditions of 25W, reaction 6h obtains saccharified solution under the conditions of 55 DEG C, pH are for 5.5.
Hydrogenation：Saccharified solution is placed in hydrogenation kettle, potassium borohydride and mass fraction that mass fraction is 0.02% is added
It is 2% Raney nickel, 2.5h is reacted under the conditions of 10MPa, 130 DEG C and obtains Fructus Hordei Germinatus oligose alcohol.
Embodiment 5
The method that a kind of utilization AMS that the present embodiment is provided prepares Fructus Hordei Germinatus oligose alcohol, it includes：
Pretreatment：By starch dissolution in the ethanol solution that volume fraction is 50%, starch is dried to obtain after ultramicro grinding
Pretreatment thing.
Liquefaction：Pretreatment of starch thing is added water the starch milk for being made into that mass fraction is 60%, add 40U/g high temperature resistant α-
Amylase, after ultrasound 2min under the conditions of 80W, reaction 5min obtains liquefied solution under the conditions of 100 DEG C, pH are for 7.
Complex enzyme is fixed：By TEOS and CTAB in molar ratio 1:0.2 is dissolved in the ammonia spirit that volume fraction is 25%,
Stirring 3h obtains the first solution.By resorcinol and hexamethylenetetramine in molar ratio 5:1.5 are dissolved in ethanol and obtain second
Solution.By the first solution and the second solution in mass ratio 3:Polymer is formed after 1 mixing.By polymer in a kettle., seal,
In 140 DEG C of solvent heat treatment 6h, precursor powder is obtained in 70 DEG C of drying after taking-up.By precursor powder flow velocity be 100mL/min
CO25h is reacted in atmosphere, reaction temperature is 650 DEG C, obtains carrier.By 1:6:1.5 ratio takes middle temperature α-shallow lake respectively
Powder enzyme, beta amylase and Pullulanase, are dispersed in water, and 30min being fixed complex enzymes are shaken under the conditions of 25 DEG C.
Saccharification：Immobilization complex enzyme is added in liquefied solution, is 6 in 60 DEG C, pH after ultrasound 9min under the conditions of 25W
Under the conditions of react 6h obtain saccharified solution.
Hydrogenation：Saccharified solution is placed in hydrogenation kettle, potassium borohydride and mass fraction that mass fraction is 0.02% is added
It is 3% Raney nickel, 2h is reacted under the conditions of 10MPa, 120 DEG C and obtains Fructus Hordei Germinatus oligose alcohol.
Comparative example 1
A kind of method for preparing Fructus Hordei Germinatus oligose alcohol that this comparative example is provided, it includes：
Liquefaction：Starch is added water the starch milk for being made into that mass fraction is 25%, adds the Thermostable α-Amylase of 40U/g,
5min is reacted under the conditions of 100 DEG C, pH are for 7 obtain liquefied solution.
Saccharification：The mesophilicα-diastase of addition 10U/g, the beta amylase of 60U/g and 20U/g's is general in liquefied solution
Shandong orchid enzyme, reaction 6h obtains saccharified solution under the conditions of 55 DEG C, pH are for 5.5.
Hydrogenation：By saccharified solution be placed in hydrogenation kettle in, add mass fraction be 2% Raney nickel, 10MPa, 130 DEG C
Under the conditions of react 2.5h obtain Fructus Hordei Germinatus oligose alcohol.
Test example 1
Constituted using the sugar alcohol of high-efficient liquid phase chromatogram technique analysis each sample, as a result as shown in table 1.
The sugar alcohol of table 1 constitutes statistical form
| Project | Sorbierite/% | Maltitol/% | Maltotriose alcohol/% | Maltotetraose alcohol/% | Maltopentaose alcohol/% |
| Embodiment 1 | 5.3 | 35.8 | 43.6 | 5.1 | 4.8 |
| Embodiment 2 | 5.6 | 39.1 | 40.2 | 4.7 | 2.3 |
| Embodiment 3 | 5.5 | 38.0 | 41.7 | 4.5 | 3.0 |
| Embodiment 4 | 5.4 | 36.9 | 40.8 | 4.9 | 2.8 |
| Embodiment 5 | 5.4 | 33.6 | 44.2 | 6.4 | 2.3 |
| Comparative example 1 | 10.6 | 30.2 | 5.1 | 1.0 | 0 |
From table 1, compared to the method for comparative example, it is few that utilization AMS provided in an embodiment of the present invention prepares malt
The method of sugar alcohol, enzymatic activity is higher, using the starch milk of high concentration, prepares the Fructus Hordei Germinatus oligose alcohol product of high-quality, especially
Be maltotriose alcohol in product content it is higher.
In sum, the method that utilization AMS provided in an embodiment of the present invention prepares Fructus Hordei Germinatus oligose alcohol, method is simple,
Parameters are easily controlled, and obtained Fructus Hordei Germinatus oligose alcohol is best in quality, and the content of maltotriose alcohol is higher.The method being capable of profit
Liquefied with high-concentration starch milk, and starch enzyme dosage and enzymolysis time, improve production efficiency can be saved.Starch milk is through resistance to
After high-temperatureα-amylase treatment, then under the complex enzyme hydrolysis effect of mesophilicα-diastase, beta amylase and Pullulanase, pass through
The synergy of various enzymes, obtains the saccharified solution of Fructus Hordei Germinatus oligose content high.
Embodiments described above is a part of embodiment of the invention, rather than whole embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of method that utilization AMS prepares Fructus Hordei Germinatus oligose alcohol, it is characterised in that comprise the following steps：
Pretreatment：By starch dispersion in the alcoholic solution that volume fraction is 30~60%, it is dried to obtain after being processed through ultramicro grinding
Pretreatment of starch thing；
Liquefaction reaction：The pretreatment of starch thing is configured to the starch milk that mass fraction is 55~65%, add high temperature resistant α-
Amylase, liquefied solution is obtained through liquefaction reaction；
Saccharification reaction：Complex enzyme is added in the liquefied solution, after ultrasonically treated 8~10min under conditions of 20~30W,
Through being saccharified, reaction obtains saccharified solution, wherein, the complex enzyme includes mesophilicα-diastase, beta amylase and Pullulanase；
Hydrogenation reaction：The saccharified solution obtains Fructus Hordei Germinatus oligose alcohol by hydrogenation reaction.
2. method according to claim 1, it is characterised in that in pre-treatment step, ultramicro grinding be processed as first 10~
Homogeneous under 15MPa pressure, then microjet homogenization is carried out under conditions of 100~130MPa of pressure, 50~60 DEG C of temperature, obtain
To the pretreatment of starch thing that average grain diameter is 20~80 μm.
3. method according to claim 1, it is characterised in that in liquefaction reaction step, adds the high temperature resistant alphalise starch
After enzyme, liquefaction reaction is carried out again after ultrasonically treated 2~3min under the conditions of 70~80W.
4. method according to claim 1, it is characterised in that the reaction condition of liquefaction reaction is：It is 95~105 in temperature
DEG C, pH be to react 4~6min under the conditions of 6.5~7.5, the consumption of the Thermostable α-Amylase is starch described in 30~45U/g
Pretreatment thing.
5. method according to claim 1, it is characterised in that the mesophilicα-diastase, the beta amylase with it is described
The amount ratio of Pullulanase is 1:5~7:0.5~2.
6. method according to claim 4, it is characterised in that saccharification reaction condition is：Temperature be 50~60 DEG C, pH be
5~7h is reacted under the conditions of 5~6, the consumption of the mesophilicα-diastase is pretreatment of starch thing described in 10~15U/g.
7. method according to claim 6, it is characterised in that in saccharification step, the complex enzyme is fixed on carrier, institute
State carrier and be prepared as follows and obtain：
By silicon source and surfactant in molar ratio 1:0.15~0.3 is dissolved in acid solution or alkaline solution, stirring reaction 2
~3h obtains the first solution；
By resorcinol and hexamethylenetetramine in molar ratio 5:1~2 is dissolved in solvent, obtains the second solution；
By the first solution and the second solution in mass ratio 2~4:1 mixing, and polymerization forms polymer, by the polymeric seal
4~8h of solvent heat treatment under the conditions of 120~150 DEG C, precursor powder is obtained after drying afterwards；
The precursor powder reacts in carbon dioxide atmosphere, reaction temperature be 600~680 DEG C, the reaction time be 3~
6h, obtains the carrier.
8. method according to claim 5, it is characterised in that the complex enzyme is with the fixing means of the carrier：Will
The complex enzyme is dispersed in water, and adds the carrier, 25~35min of concussion reaction under the conditions of 20~30 DEG C.
9. method according to claim 1, it is characterised in that in hydrogenation reaction step, in the saccharification reaction solution
It is 0.01~0.03% potassium borohydride and catalyst that mass fraction is 2~3% to add mass fraction, 8~10MPa,
Hydrogenation reaction is carried out under the conditions of 120~135 DEG C.
10. a kind of Fructus Hordei Germinatus oligose alcohol, it is characterised in that the method according to claim 1~9 any one is prepared.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384985A (en) * | 2017-09-20 | 2017-11-24 | 无锡甜丰食品有限公司 | A kind of Mashing process of high concentration maltodextrin |
| CN112048531A (en) * | 2020-09-07 | 2020-12-08 | 广州善合化工有限公司 | Maltooligosaccharide glucoside and preparation method and application thereof |
| CN113502037A (en) * | 2021-08-16 | 2021-10-15 | 苏州科宁多元醇有限公司 | Novel polyhydric sugar alcohol plasticizer, preparation method thereof and application thereof in preparation of starch-based degradable material |
| US11549133B2 (en) * | 2020-01-17 | 2023-01-10 | Jiangnan University | Preparation method of amylodextrin |
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2017
- 2017-04-19 CN CN201710259777.3A patent/CN106906266B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384985A (en) * | 2017-09-20 | 2017-11-24 | 无锡甜丰食品有限公司 | A kind of Mashing process of high concentration maltodextrin |
| US11549133B2 (en) * | 2020-01-17 | 2023-01-10 | Jiangnan University | Preparation method of amylodextrin |
| CN112048531A (en) * | 2020-09-07 | 2020-12-08 | 广州善合化工有限公司 | Maltooligosaccharide glucoside and preparation method and application thereof |
| CN112048531B (en) * | 2020-09-07 | 2022-02-11 | 广州善合化工有限公司 | Maltooligosaccharide glucoside and preparation method and application thereof |
| CN113502037A (en) * | 2021-08-16 | 2021-10-15 | 苏州科宁多元醇有限公司 | Novel polyhydric sugar alcohol plasticizer, preparation method thereof and application thereof in preparation of starch-based degradable material |
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