CN106906179A - A kind of Seedling height versatile stem cell culture medium - Google Patents
A kind of Seedling height versatile stem cell culture medium Download PDFInfo
- Publication number
- CN106906179A CN106906179A CN201710294842.6A CN201710294842A CN106906179A CN 106906179 A CN106906179 A CN 106906179A CN 201710294842 A CN201710294842 A CN 201710294842A CN 106906179 A CN106906179 A CN 106906179A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- culture
- stem cell
- concentration
- sodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/46—Amines, e.g. putrescine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2306—Interleukin-6 (IL-6)
Abstract
The invention belongs to stem cells technology field, and in particular to a kind of Seedling height versatile stem cell culture medium.Should the composition of culture medium be：DMEM/F12 culture mediums;0.5 ~ 0.9mg/L niacinamides, 22 ~ 25mg/L FGF 2,0.001 ~ 0.003mg/L sodium selenates, 0.2 ~ 0.3mg/L sodium acid carbonates, 350 ~ 400 μ g/L ironic citrates, 200 ~ 300 μ g/L IL 6 and 50 ~ 60 μ g/L putrescines；The concentration of said components is with added to the densimeter in DMEM/F12 culture mediums.The present invention can be for commercial medium, with significantly high growth efficiency, and gained culture medium is more suitable to the culture of versatile stem cell.
Description
Technical field
The invention belongs to stem cells technology field, and in particular to a kind of Seedling height versatile stem cell culture medium.
Background technology
Field, the TeSR-E8 culture mediums of Stemcell companies and Life companies are developed in the culture medium of versatile stem cell
E8 culture mediums be more ripe versatile stem cell culture medium.
The shortcoming that TeSR-E8 culture mediums are primarily present is included but is not limited to：（1）Promotion is added with TeSR-E8 culture mediums
The micromolecular compound or beta -mercaptoethanol of reprogramming, it can cause obvious cytotoxicity, limit versatile stem cell
Low density cell culture；（2）Cytokine content in TeSR-E8 culture mediums is higher, causes the cost of the culture medium very high.
Therefore, exploitation can promote versatile stem cell culture and the less culture medium of additive used is grinding for this area
Originating party to one of.However, existing research does not obtain the achievement for working well also.Only know that ironic citrate can be to a certain extent at present
Replace the people's apotransferrin in TeSR-E8 culture mediums.
In terms of the culture of stem cell is promoted, usual way is control environmental factor（Such as oxygen content, culture space）With
Add some function factors（Such as EGF）.《Enhancement of human neural stem cell self-renewal
in 3D hypoxic culture》By the dimension, oxygen content and the certain density EGF and FGF-2 of addition that control culture space
Promote the increment and differentiation of hNSCs.The maintenance for promoting stem cell function can be played by adding appropriate active oxygen,
On the basis of this,《Active oxygen is to induction versatile stem cell（iPS）Effect and functional study》Compare further investigation.
《Substance P enhances proliferation and paracrine potential of adipose-
derived stem cells in vitro 》Have studied the influence of in vitro cultures of the Substance P for ADSCs.
In addition, more urgent at present is the research and development on the culture medium without animal product.In this respect, CN
103952374 B provide a kind of good thinking, and have obtained good effect.But, it has the disadvantage that addO-on therapy is excessive,
Relatively costly, industrialization prospect is poor.
In terms of serum-free stem cell media, the addition of Sodium Pyruvate is important, such as Application No.
201310134502.9 Chinese patent《A kind of fat mesenchymal stem cell culture medium of serum-free》And Application No.
201410018678.2 Chinese patent《Cultivate the serum free medium of placenta mesenchyma stem cell》In with the addition of pyruvic acid
Sodium.
The Chinese patent of Application No. 201610570585.X make use of putrescine as one of active factors, and match somebody with somebody
Close other adding ingredients and prepare a kind of culture medium that can promote stem cell proliferation speed.The material is in Application No. application
Number for 201210564154.4 Chinese patent in be equally used, can be used for cultivate and amplification of mesenchymal stem cells.
In some known stem cell serum-free culture mediums, niacinamide is also by as one of additive.Such as application number
For the Chinese patent of 201611087240 .5 make use of niacinamide as additive.But the patent need to add Ultroser
G Xue Cheongju substitutes could realize the serum-free of culture medium, relatively costly.
In sum, this area needs one kind badly can promote the value-added serum free medium of versatile stem cell.
The content of the invention
The purpose of the present invention aims to solve the problem that a kind of Seedling height versatile stem cell culture medium of art technology problem, its
Composition is：
DMEM/F12 culture mediums;
0.5 ~ 0.9 mg/L niacinamides, 22 ~ 25 mg/L FGF-2,0.001 ~ 0.003 mg/L sodium selenates, 0.2 ~ 0.3 mg/
L sodium acid carbonates, 350 ~ 400 μ g/L ironic citrates, 200 ~ 300 μ g/L IL-6 and 50 ~ 60 μ g/L putrescines；
The concentration of said components is with added to the densimeter in DMEM/F12 culture mediums.
Preferably, the concentration of the ironic citrate is 360 ~ 380 μ g/L.
Preferably, the concentration of the niacinamide is 0.6 ~ 0.8 mg/L.
Preferably, the concentration of the FGF-2 is 23 mg/L.
Preferably, the concentration of the sodium selenate is 0.002 mg/L.
Preferably, the concentration of the IL-6 is 260 μ g/L.
The culture medium is using preceding configuration, in preservation at 0-8 DEG C.
It is a discovery of the invention that the Sodium Pyruvate of prior art report can be replaced using putrescine and sodium selenate, not
While influence is multi-functional, can cause that versatile stem cell is bred faster.
Fibroblast growth factor is commonly used for the adding ingredient of cell culture medium, either in multi-functional stem cell and
In the culture medium of announcement of other stem cells, one of main additive is taken as（Such as the .5 of Chinese patent 201611087240
With the .9 of Chinese patent 201610508983）.
In the present invention, inventor is had found using the FGF-2m of addition as described above（FGF-2）With it is white
Interleukin -6（IL-6）, then it is aided with putrescine, sodium selenate and the niacinamide of addition as described above, just can realize preferably many
The value-added effect of function stem cell.On this basis, the ironic citrate of addition as described above is added, replacement TeSR-E8 can be played
In people's apotransferrin effect, it is to avoid to adding people's apotransferrin or recombinant human lactoferrin egg in culture medium
In vain.
As shown in a comparative example of the invention, when putrescine is lacked, cell is raw after culture 84 hours
Declining to a great extent occurs in speed long, the too early entrance growth platform phase.
In addition, after sodium selenate is replaced with into Sodium Pyruvate, there is decline by a relatively large margin in the value-added speed of cell.
What deserves to be explained is, culture medium of the invention is preferable for the culture effect of versatile stem cell, but for other
The culture effect of stem cell is but without so excellent.
In producing the partly cause of above-mentioned situation to may is that different stem cells, its increment and some critical functions are influenceed
The factor it is different.
At present, with the rise of gene editing technology（Relevant report is such as《The concerted action of type 2
and type 3 deiodinases regulates the cell cycle and survival of basal cell
carcinoma cells》With《ERK Kinases Phosphorylate Lin28a to Modulate P19 Cell
Proliferation and Differentiation》）, the governing factor of increment and biological function for different cells
To be constantly found, more solid theoretical foundation can be provided for the research of the culture medium of different stem cells in the future.
Brief description of the drawings
Fig. 1 is cell growth curve figure of the culture medium of the present invention to versatile stem cell culture；
Fig. 2 is cell growth curve figure of the culture medium of contrast groups 1 to versatile stem cell culture.
Specific embodiment
With reference to embodiments further the present invention will be described, be worth mentioning when, following embodiments are functioned only as
Example is acted on, it is not intended that the present invention has been limited, it should be appreciated by those skilled in the art every to meet spirit of the invention
Technical scheme belong to protection scope of the present invention.
Embodiment 1
DMEM/F12 culture mediums;
0.5 mg/L niacinamides, 22 mg/L FGF-2,0.002 mg/L sodium selenates, 0.2 mg/L sodium acid carbonates, 400 μ g/
L ironic citrates, 300 μ g/L IL-6 and 50 μ g/L putrescines；
The concentration of said components is with added to the densimeter in DMEM/F12 culture mediums.
Embodiment 2
DMEM/F12 culture mediums;
0.9 mg/L niacinamides, 25 mg/L FGF-2,0.001 ~ 0.003 mg/L sodium selenates, 0.3 mg/L sodium acid carbonates,
400 μ g/L ironic citrates, 200 μ g/L IL-6 and 60 μ g/L putrescines；
The concentration of said components is with added to the densimeter in DMEM/F12 culture mediums.
Embodiment 3
DMEM/F12 culture mediums;
0.6 mg/L niacinamides, 23 mg/L FGF-2,0.002 mg/L sodium selenates, 0.2 ~ 0.3 mg/L sodium acid carbonates, 360
μ g/L ironic citrates, 260 μ g/L IL-6 and 55 μ g/L putrescines；
The concentration of said components is with added to the densimeter in DMEM/F12 culture mediums.
Comparative example 1
In addition to without putrescine, remaining is consistent with embodiment 3.
Comparative example 2
Outside sodium selenate only replaced with into Sodium Pyruvate, remaining is consistent with embodiment 3.
Application Example 1
To the IPS cell line HIPS of recovery people, it is seeded in Matrigel culture dishes, the culture of the embodiment 3 that addition has been configured
Base, carries out changing liquid culture daily, and condition of culture is：Temperature is 37 DEG C, 5% CO2Atmosphere.When versatile stem cell degree of converging is arrived
When 70%, digestion process is carried out, carry out Secondary Culture, 40 generations of continuous culture.Increment curve is drawn, as shown in Figure 1.
Comparative example 2 is processed with same processing mode, 1 is organized as a comparison.Increment curve is drawn, as shown in Figure 2.
Comparative example 1 is processed with same processing mode, 2 are organized as a comparison.It was found that at the 3rd day, number of cells was
800000/per hole or so, after the 3.5th day, declining to a great extent occurs in the growth rate of cell, and to after the 4th day, does not grow substantially,
Number of cells is 1,800,000/every hole or so.
As shown in Figure 1, it is of the invention for commercial medium, with significantly high growth efficiency, gained culture medium
More it is suitable to the culture of versatile stem cell.
From Fig. 2 and the culture situation of contrast groups, the increasing of the selection for versatile stem cell of additive of the invention
Value has significant impact.
Application Example 2
Using the medium culture mesenchymal stem cells of embodiment 3, compared with the conventional medium containing hyclone, gained cell
Number fail to reach its 60%.
Claims (8)
1. a kind of Seedling height versatile stem cell culture medium, it is characterised in that the composition of the culture medium is：
DMEM/F12 culture mediums;
0.5 ~ 0.9 mg/L niacinamides, 22 ~ 25 mg/L FGF-2,0.001 ~ 0.003 mg/L sodium selenates, 0.2 ~ 0.3 mg/
L sodium acid carbonates, 350 ~ 400 μ g/L ironic citrates, 200 ~ 300 μ g/L IL-6 and 50 ~ 60 μ g/L putrescines；
The concentration of said components is with added to the densimeter in DMEM/F12 culture mediums.
2. culture medium according to claim 1, it is characterised in that the concentration of the putrescine is 55 μ g/L.
3. culture medium according to claim 1 and 2, it is characterised in that in the culture medium, the concentration of the ironic citrate
It is 360 ~ 380 μ g/L.
4. culture medium according to claim 3, it is characterised in that in the culture medium, the concentration of the niacinamide is
0.6~0.8 mg/L。
5. culture medium according to claim 4, it is characterised in that in the culture medium, the concentration of the FGF-2 is 23
mg/L。
6. culture medium according to claim 5, it is characterised in that in the culture medium, the concentration of the sodium selenate is
0.002 mg/L。
7. culture medium according to claim 5, it is characterised in that in the culture medium, the concentration of the IL-6 is 260 μ
g/L。
8. culture medium according to claim 1, it is characterised in that the culture medium using preceding configuration, in being protected at 0-8 DEG C
Deposit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710294842.6A CN106906179A (en) | 2017-04-28 | 2017-04-28 | A kind of Seedling height versatile stem cell culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710294842.6A CN106906179A (en) | 2017-04-28 | 2017-04-28 | A kind of Seedling height versatile stem cell culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106906179A true CN106906179A (en) | 2017-06-30 |
Family
ID=59211294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710294842.6A Pending CN106906179A (en) | 2017-04-28 | 2017-04-28 | A kind of Seedling height versatile stem cell culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106906179A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009147400A1 (en) * | 2008-06-05 | 2009-12-10 | Iti Scotland Limited | Stem cell culture media and methods |
| CN102234627A (en) * | 2010-04-30 | 2011-11-09 | 中国科学院广州生物医药与健康研究院 | Culture medium additive and application thereof |
| CN103255103A (en) * | 2013-04-17 | 2013-08-21 | 广州爱菲科生物科技有限公司 | Serum-free adipose tissue-derived mesenchymal stem cell culture medium |
| CN103881971A (en) * | 2012-12-21 | 2014-06-25 | 曾因明 | Culture medium and culture method for culturing and/or amplifying mesenchymal stem cells |
| CN103952374A (en) * | 2014-05-07 | 2014-07-30 | 北京赛贝生物技术有限公司 | Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells |
| CN105907705A (en) * | 2016-06-28 | 2016-08-31 | 广州市搏克生物技术有限公司 | Multi-potent stem cell culture medium |
| CN106190946A (en) * | 2016-07-19 | 2016-12-07 | 安徽惠恩生物科技股份有限公司 | A kind of culture medium for expansion of stem cells |
| CN106591295A (en) * | 2016-12-31 | 2017-04-26 | 成都育芽科技有限公司 | Multifunctional stem cell culture method and used culture medium |
-
2017
- 2017-04-28 CN CN201710294842.6A patent/CN106906179A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009147400A1 (en) * | 2008-06-05 | 2009-12-10 | Iti Scotland Limited | Stem cell culture media and methods |
| CN102234627A (en) * | 2010-04-30 | 2011-11-09 | 中国科学院广州生物医药与健康研究院 | Culture medium additive and application thereof |
| CN103881971A (en) * | 2012-12-21 | 2014-06-25 | 曾因明 | Culture medium and culture method for culturing and/or amplifying mesenchymal stem cells |
| CN103255103A (en) * | 2013-04-17 | 2013-08-21 | 广州爱菲科生物科技有限公司 | Serum-free adipose tissue-derived mesenchymal stem cell culture medium |
| CN103952374A (en) * | 2014-05-07 | 2014-07-30 | 北京赛贝生物技术有限公司 | Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells |
| CN105907705A (en) * | 2016-06-28 | 2016-08-31 | 广州市搏克生物技术有限公司 | Multi-potent stem cell culture medium |
| CN106190946A (en) * | 2016-07-19 | 2016-12-07 | 安徽惠恩生物科技股份有限公司 | A kind of culture medium for expansion of stem cells |
| CN106591295A (en) * | 2016-12-31 | 2017-04-26 | 成都育芽科技有限公司 | Multifunctional stem cell culture method and used culture medium |
Non-Patent Citations (3)
| Title |
|---|
| GHOURICHAEE SS 等: "Enhancement of human neural stem cell self-renewal in 3D hypoxic culture", 《BIOTECHNOL BIOENG》 * |
| 任丽伟 等: "小鼠iPSCs诱导分化为胰岛素分泌细胞及治疗糖尿病的实验研究", 《中国病理生理杂志》 * |
| 赵宗江 主编: "《细胞生物学》", 31 December 2016 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103805562B (en) | Cultivate the serum free medium of placenta mesenchyma stem cell | |
| Gazdar et al. | Establishment of continuous, clonable cultures of small-cell carcinoma of the lung which have amine precursor uptake and decarboxylation cell properties | |
| CN109762782B (en) | Culture medium for promoting growth of mesenchymal stem cells and preparation method thereof | |
| CN102433302B (en) | Serum-free culture medium for mesenchymal stem cells | |
| CN102634482B (en) | Serum-free complete medium for mesenchymal stem cell | |
| CN106635978B (en) | A kind of umbilical cord mesenchymal stem cells serum free medium and its preparation method and application | |
| CN105112362B (en) | A kind of serum free medium of placenta mesenchyma stem cell and preparation method thereof | |
| KR20120008223A (en) | Medium for culturing mesenchymal stem cells derived from amnion and method for culturing mesenchymal stem cells derived from amnion using thereof | |
| CN105112364A (en) | Serum-free medium for human adipose-derived stem cells and preparation method thereof | |
| CN111826348B (en) | In-vitro efficient preparation method and application of mesenchymal stem cells derived from human induced pluripotent stem cells | |
| KR20120082626A (en) | Compositions for suspension culture of stem cells | |
| CN107653225A (en) | A kind of culture medium and its amplification method for being used to expand human mesenchymal stem cell | |
| CN106754675A (en) | A kind of fat stem cell serum free medium and its production and use | |
| CN103497892B (en) | A kind of cell cultures base material and its preparation method and application | |
| CN105112363A (en) | Serum-free medium for human adipose-derived stem cells and preparation method thereof | |
| CN103146645B (en) | Method for inducing mesenchymal stem cells (MSCs) into chondrocytes | |
| CN104830763A (en) | Application of Y-27632 in cultivation of mesenchymal stem cells and cultivation method of mesenchymal stem cells | |
| CN114561358B (en) | Enhanced infection culture medium and method for improving infection rate of cell lentivirus | |
| CN112725270A (en) | Human-derived bone marrow mesenchymal stem cell induction culture medium and induction method | |
| CN103695367A (en) | Improved culture medium for neonatal pig islet cells and use method of same | |
| CN115896010A (en) | Application of compound in improving activity of umbilical cord mesenchymal stem cells | |
| CN114107187B (en) | Dental pulp mesenchymal stem cell culture medium and method for applying same | |
| CN113151165B (en) | Culture medium and culture method for human umbilical cord mesenchymal stem cell amplification | |
| KR20160002490A (en) | Culture medium composition for promoting stem cell proliferation and methods for culturing stem cell | |
| CN112391340A (en) | Mesenchymal stem cell culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170630 |