CN106906147A - It is adapted to the culture medium of ustilaginoidea virens growth - Google Patents
It is adapted to the culture medium of ustilaginoidea virens growth Download PDFInfo
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- CN106906147A CN106906147A CN201710191224.9A CN201710191224A CN106906147A CN 106906147 A CN106906147 A CN 106906147A CN 201710191224 A CN201710191224 A CN 201710191224A CN 106906147 A CN106906147 A CN 106906147A
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- ustilaginoidea virens
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- 241001474928 Ustilaginoidea virens Species 0.000 title claims abstract description 72
- 230000012010 growth Effects 0.000 title claims abstract description 50
- 229920002148 Gellan gum Polymers 0.000 claims abstract description 40
- 239000000216 gellan gum Substances 0.000 claims abstract description 39
- 235000010492 gellan gum Nutrition 0.000 claims abstract description 39
- 239000001888 Peptone Substances 0.000 claims abstract description 29
- 108010080698 Peptones Proteins 0.000 claims abstract description 29
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 29
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- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 12
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- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 12
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- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 11
- 239000011591 potassium Substances 0.000 claims abstract description 11
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- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of culture medium of suitable ustilaginoidea virens growth, culture medium main body is into being grouped into by following weight content：Sucrose 0.1% 5%, peptone 0.1% 5%, gellan gum 0.1% 1%, Mg2+0.02% 0.1%, balance of water.Micro diphosphonic acid hydrogen potassium, sodium chloride, ferrous sulfate, sodium molybdate, manganese sulfate, calcium carbonate these inorganic salts are added in above-mentioned culture medium main body, the culture medium of suitable ustilaginoidea virens growth is obtained.
Description
Technical field
It is to be related to a kind of culture medium for being suitable to ustilaginoidea virens culture specifically the present invention relates to biological technical field.
Background technology
Ustilaginoidea virens Perfect stage is Claviceps oryzae-sativae Hashioka, belongs to Ascomycota wheat
Angle Cordycepps fungi, Invisible element is Ustilaginoidea virens (Cooke) Takahashi, different name：U.oryzae(Pat)
Bref, belongs to the green pyrenomycetes category of asexual mushroom, mainly infects the fungal disease that Rice Panicle causes.Pathological research discovery,
U.virens is invaded from the lemma of small ear and glumelle gap, formed more than the rice curve of grain and surface cover substantial amounts of yellow or
Blackish green chlamydospore, infected seed center is the white meat mass of the intensive composition of hypohostroma, and its periphery is because producing chlamydospore
Mycelia maturity is different, can be divided into three layers：Outer layer is ripe earliest, in blackish green or olive-green；The second layer is orange-yellow；3rd
Layer is yellow.False smut is also known as false smut, green smut, blue or green powder disease, paddy blossom disease etc..China is early in Ming Dynasty's Li Shizhen (1518-1593 A.D.)《Book on Chinese herbal medicine
Detailed outline》In just have and refer to that ustilaginoidea virens fructification is the record of " fungus-infected rice spike ".
The disease has generation in various degree in more than 30 countries in Asia, America and Africa and each rice region of China.General fringe
The incidence of disease is 4%~6%, and seriously up to more than 50%, the grain incidence of disease is 0.2%~0.4%, high reachable more than 5%.It is not only
Increase not plump paddy rate, green rice-grain rate, broken rice rate, the local field underproduction 20%~30%, can produce has hypertoxic effect to people and livestock
Mycotoxin, and people can be caused when 0.5% infected seed is contained in paddy, poultry poisoning.Since 20 century 70s, due to
The a large amount of of the change of rice varieties, the change of cropping system and chemical fertilizer use, and hybrid rice the factor, rice such as appearance
The generation and harm of bent disease are in exacerbation trend, it has also become one of Chinese and many rice regions in the world Major Diseases.
Ustilaginoidea virens is slow-growing, the inefficiency in its cultured in vitro research, and culture effect is undesirable, it is impossible to reach
Preferable culture effect, is unfavorable for that related fields research work is in progress.It is well known that the effect of fungal culture, economy and effect
What rate played a decisive role is culture medium, and the optimization of culture medium is the core link for setting up efficient fungal culture process of the test.
Therefore, the nutritional need according to ustilaginoidea virens during growth, metabolism and experiment growth table not of the same race reach, designs false smut
The composition of nutrients, content and proportioning are the important contents of work in bacterium culture medium.
In the market lacks the culture medium of special culture ustilaginoidea virens, and the ustilaginoidea virens culture medium used by laboratory is culture one
As fungi culture medium finished product, ustilaginoidea virens is slow-growing on finished product culture medium common on the market, it is impossible to meet experiment
High efficiency requirement.The constituent of fungi culture medium is nothing more than carbon source, nitrogen source, inorganic salts and coagulator；Carbon source is carbon hydrate
Thing, nitrogen source is protein, amino acid, and they are the precursor substances of thalli growth and metabolism institute required material, can synthesize fungi from
Essential biology enzyme in structural proteins and biochemical reaction needed for body, also directly participates in the biological conjunction of secondary metabolite
Into, at the same be also synthesize other biological molecule Metabolic Intermediate, be alternatively arranged as substrate provide cellular physiological events necessary to
Energy, is the important nutrient of cell growth, metabolism and product production.Inorganic salts contain phosphoric acid buffer thing, Mg2+, NaCl,
Fe2+Deng inorganic ion, Ph values are 7.0, and major function of the inorganic salts in microbial life activity has：Constitute thalline into
Point, as the activity of the part or maintenance enzyme of coenzyme or enzyme, regulation Premeabilisation of cells pressure, hydrogen ion concentration, redox electricity
Position etc., some are the activator of some enzymes such as protease.Coagulator is the necessary composition of solid medium, play make culture medium its
The solidification of his composition, is conducive to pathogen growth, and water conservation effect.
Investigate according to the study, do the ustilaginoidea virens culture medium used by the related research institute of ustilaginoidea virens culture and be mostly in the market
Commercially available culture medium, potato sucrose agar medium, potato dextrose agar coerces Ben Zheshi culture mediums and rice
From culture medium, when the concrete condition of culture was for culture 30 days, ustilaginoidea virens mycelial growth situation is, PDA culture medium：60.3mm；
PSA culture mediums：69.8mm；Side of body Ben Zheshi culture mediums：59.6mm；Big grain of rice culture medium：65.9mm, ustilaginoidea virens is cultivated at these
Slow-growing on base, this science investigation and study on prevention to ustilaginoidea virens brings great inconvenience, and inefficiency is research rice
One threshold of bent germ.
Gellan gum can be used as thickener, stabilizer.
2011104555227 invention is informed《A kind of culture medium of the difficult culture bacterium of culture of isolated》, its formula is：Often
Add 0.3mL Freamine Ⅲs, 10 μ L solution Is (trace element), 0.3mL solution IIs (organic in 100mL VL55 culture mediums
Acid), 0.3mL solution IIIs (organic carbohydrate) and 1.5wt% gellan gums (gellan gum).The culture medium can be separated efficiently
A large amount of difficult culture bacteriums.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of culture medium of suitable ustilaginoidea virens growth, i.e. provide a kind of
It is exclusively used in the peptone sucrose gellan gum culture medium P.S.G of ustilaginoidea virens culture.
In order to solve the above-mentioned technical problem, the present invention provides a kind of culture medium of suitable ustilaginoidea virens growth, culture medium master
Body is into being grouped into by following weight content (%)：
The improvement of the culture medium grown as suitable ustilaginoidea virens of the invention：Two phosphorus are added in 1kg culture medium main bodys
25 ± 3mg of potassium hydrogen phthalate, 125 ± 10mg of sodium chloride, 5 ± 1mg of ferrous sulfate, 5 ± 1mg of sodium molybdate, 5 ± 1mg of manganese sulfate, calcium carbonate
100 ± 10mg, obtains the culture medium of suitable ustilaginoidea virens growth.
The further improvement of the culture medium grown as suitable ustilaginoidea virens of the invention：Mg2+It is magnesium sulfate or magnesium sulfate
Hydrate.
The further improvement of the culture medium grown as suitable ustilaginoidea virens of the invention：In culture medium main body, sucrose is
1%, peptone is 2%, and gellan gum is 0.3%, Mg2+It is 0.02%.
The further improvement of the culture medium grown as suitable ustilaginoidea virens of the invention：Water is ultra-pure water.
The present invention is intended to provide ustilaginoidea virens fast-growth culture medium (that is, being suitable to the culture medium of ustilaginoidea virens growth), tool
Body is related to the application of culture medium prescription and gellan gum in terms of fungal culture.
Inventor, first according to the ratio of conventional culture medium each component, selects different compositions during invention
Formula combination, with time culture with a collection of ustilaginoidea virens, pathogen growth situation is recorded according to test situation into culture medium, is therefrom sieved
The optimal culture medium of ustilaginoidea virens growing state is selected, and replaces agar using gellan gum as coagulator, and it is suitable by addition
The Mg of content2+To realize solving the technical matters that gellan gum is difficult solidification, screening obtains optimal ustilaginoidea virens culture medium 21 days
72.5mm can be grown to, hence it is evident that improve culture efficiency.
In the present invention, sucrose (C12H22O11) used as carbon source, predominantly ustilaginoidea virens growth and biochemical metabolism is expressed and provided
Necessary energy supply；Peptone serves not only as nitrogen source, can also be the growth factor needed for growth of microorganism offer；Inorganic salts are
The mixture of the inorganic ions such as P, Mg, Na, to be adapted to the gellan gum of growth of microorganism as coagulator.
In the present invention, Mg2+Addition be enough to solidify gellan gum.Ion concentration, especially Mg2+Concentration can be right
The growth of ustilaginoidea virens affects, and the growing state of ustilaginoidea virens is different under the ionic condition of various concentrations, this hair
It is bright to find the Mg for being most suitable for ustilaginoidea virens growth2+Concentration, while can solidify gellan gum, ustilaginoidea virens can also with compared with
Good state growth.Compared with the culture medium of other culture fungies, P.S.G of the invention can significantly improve the growth of ustilaginoidea virens
Speed, improves the effect of false smut growth.
The present invention has following technical advantage：
(1) culture medium of the invention can effectively improve the growth rate of ustilaginoidea virens, needed for shortening culture ustilaginoidea virens
Time；
(2) the false smut mycelia health cultivated, is not in underfed situation；
(3) culture medium ingredient of the invention, raw materials easily obtain, it is cheap, with using into well
Sheet and economy；
(4) culture medium of the invention is prepared and easy to use, is suitable to large-scale production and is used.
(5) gellan gum is substituted agar by culture medium of the invention, and other like products of gellan gum are compared to tool
There is consumption few, elasticity, hardness and freezing point, fusing point are adjustable, the gel of formation has intensity and transparency very high, thermally-stabilised
Property good and acid and alkali-resistance the features such as.
Specific embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Embodiment 1, a kind of culture medium P.S.G of suitable ustilaginoidea virens growth, the culture medium main body is by following weight content
(%) into being grouped into：Sucrose 1%, peptone 2%, gellan gum 0.3%, Mg2+0.02%, balance of water (ultra-pure water)；Mg2+
It is magnesium sulfate.
The inorganic salts of following content are added in above-mentioned culture medium main body：Diphosphonic acid hydrogen potassium 0.0025%, sodium chloride
0.0125%, ferrous sulfate 0.0005%, sodium molybdate 0.0005%, manganese sulfate 0.0005%, calcium carbonate 0.01%.
That is, diphosphonic acid hydrogen potassium 25mg, sodium chloride 125mg, ferrous sulfate 5mg, sodium molybdate are added in 1kg culture medium main bodys
5mg, manganese sulfate 5mg, calcium carbonate 100mg, obtain the culture medium of suitable ustilaginoidea virens growth.
Culture medium P.S.G as conventional medium, using preceding needing to carry out conventional high-temperature sterilization (in 1.1 air
Pressure, sterilize 20min at 121 DEG C).
The experiment one, culture studies using ustilaginoidea virens：
1st, culture medium is prepared
It is higher than the ustilaginoidea virens growth rate of other medium cultures in order to confirm basal culture medium invention, select potato grape
Sugared agar medium (PDA), potato sucrose agar medium (PSA), ocean broth agar culture medium (MBA), nutrient broth
Agar medium (NBA), bacteriolyze broth agar culture medium (LBA), yeast glucose agar medium (Ye.G.A), yeast cane sugar
Agar medium (Ye.S.A), yeast cefoxitin-cycloserine-fructose agar culture medium (Ye.F.A), yeast starch agar medium (Ye.D.A), albumen
Peptone glucose agar medium (P.G.A), peptone sucrose agar culture medium (P.S.A), peptone cefoxitin-cycloserine-fructose agar culture medium
(P.F.A), peptone starch agar medium (P.D.A), peptone sucrose agar culture medium (P.S.A), peptone fructose fine jade
Fat culture medium (P.F.A), leucine starch agar medium (L.D.A), leucine sucrose agar culture medium (L.S.A), bright ammonia
Tart fruit sugar agar medium (L.F.A), leucine starch sugar agar medium (L.D.A), glutamic acid starch agar medium
(G.D.A), glutamic acid sucrose agar culture medium (G.S.A), glutamic acid cefoxitin-cycloserine-fructose agar culture medium (G.F.A), glutamic acid starch sugar
Agar medium (G.D.A), this 24 kinds of culture mediums of yeast fructose gellan gum culture medium (Ye.F.G) and peptone sugarcane of the invention
Sugared gellan gum culture medium (P.S.G), the concentration of formula culture medium each component used is the optimum concentration for cultivating ustilaginoidea virens, will
The same ware ustilaginoidea virens of deployed culture medium inoculated, sets two groups of repetitions, cultivates 21 days diameters of observation bacteria cake growth, calculates
Its growth rate.
Condition of culture is：25 DEG C of constant temperature, lucifuge, quiescent culture.
Diameter length (mm)/21 (d) of average growth rate (mm/d)=balanced growth
2nd, shown in the following Tables 1 and 2 of result of the test.
The existing commercially available culture medium cultivation results of table 1, in the market
Table 2, formula medium culture result
Different culture media is illustrated with the comparative test result of culture medium P.S.G of the invention culture ustilaginoidea virens, of the invention
The ustilaginoidea virens of culture medium P.S.G cultures is high more than with the ustilaginoidea virens growth rate of other medium cultures, therefore, this culture
Base can effectively improve the growth rate of ustilaginoidea virens.
Implement two, P.S.G culture separate sources ustilaginoidea virens
1st, experiment material source
False smut bacterial strain picks up from Shandong (SD), and Jiangsu (JS), Zhejiang (ZJ01, ZJ02) is isolated after purification, uses albumen
Peptone sucrose gellan gum culture medium (P.S.G) and potato dextrose agar (PDA) cultivate four kinds from different regions
False smut, culture medium is prepared with embodiment 1, and two repetitions of every group of setting are cultivated 21 days diameters of observation bacteria cake growth, calculate it
Growth rate.
Diameter length (mm)/21 (d) of average growth rate (mm/d)=balanced growth
2nd, result of the test is as described in table 3 below and table 4.
Table 3, peptone sucrose gellan gum culture medium (P.S.G) cultivation results
Table 4, potato dextrose agar (PDA) cultivation results
Find out from the result of the test of the ustilaginoidea virens of different medium culture separate sources, peptone sucrose gellan gum culture
Growth of the base (P.S.G) to the ustilaginoidea virens of separate sources is improved the effect of growth efficiency, can cultivate various area collections
Ustilaginoidea virens.
Experiment three, difference Mg2+Influence to ustilaginoidea virens
1st, culture medium is prepared
In order to verify difference Mg in culture medium2+Concentration grows to ustilaginoidea virens, with yeast fructose gellan gum culture medium
(Ye.F.G) with peptone sucrose gellan gum culture medium (P.S.G) based on culture medium, respectively add Mg2+0.02%, 0.05%,
0.1%, i.e., it is specific as follows：
Yeast fructose gellan gum culture medium (Ye.F.G)：Culture medium main body is that fructose is 1%, and yeast is 2%, and gellan gum is
0.3%, Mg2+Respectively 0.01%, 0.02%, 0.05%, 0.1%, balance of water；Following content is added in culture medium main body
Inorganic salts：Diphosphonic acid hydrogen potassium 0.0025%, sodium chloride 0.0125%, ferrous sulfate 0.0005%, sodium molybdate 0.0005%, sulphur
Sour manganese 0.0005%, calcium carbonate 0.01%.
Peptone sucrose gellan gum culture medium (P.S.G)：Culture medium main body is that sucrose is 1%, and peptone is 2%, is tied cold
Glue is 0.3%, Mg2+Respectively 0.01%, 0.02%, 0.05%, 0.1%, balance of water；Below being added in culture medium main body
The inorganic salts of content：Diphosphonic acid hydrogen potassium 0.0025%, sodium chloride 0.0125%, ferrous sulfate 0.0005%, sodium molybdate
0.0005%, manganese sulfate 0.0005%, calcium carbonate 0.01%.
Above-mentioned Mg2+It is magnesium sulfate.
By the deployed same ware ustilaginoidea virens of culture medium inoculated, 21 days diameters of observation bacteria cake growth are cultivated, calculate it
Growth rate.
Diameter length (mm)/21 (d) of average growth rate (mm/d)=balanced growth
2nd, result of the test is as described in table 5 below, table 6.
Table 5, peptone sucrose gellan gum culture medium (P.S.G)
Table 6, yeast fructose gellan gum culture medium (Ye.F.G)
Different Mg2+Concentration can be seen that Mg to the influence result of the test that ustilaginoidea virens grows2+Concentration is higher, ustilaginoidea virens
Growth rate is smaller, and efficiency is lower, so Mg2+Ustilaginoidea virens growth can be affected, concentration is 0.02% Mg2 +It is that gellan gum can both solidified, and it is minimum to ustilaginoidea virens growth effect, it is to cultivate optimal dense used by false smut culture medium
Degree.
From identical Mg2+Yeast fructose gellan gum culture medium (Ye.F.G) and peptone sucrose gellan gum culture medium
(P.S.G) cultivation results understand that ustilaginoidea virens is cultivated on P.S.G culture medium energy more efficient ground.
Experiment four, the formula of culture medium P.S.G is made into it is as follows：
1# culture mediums P.S.G：
Culture medium main body is：Sucrose 5%, peptone 5%, gellan gum 1%, Mg2+0.1%, balance of water；In culture medium master
The inorganic salts of following content are added in body：Diphosphonic acid hydrogen potassium 0.0025%, sodium chloride 0.0125%, ferrous sulfate 0.0005%,
Sodium molybdate 0.0005%, manganese sulfate 0.0005%, calcium carbonate 0.01%.
2# culture mediums P.S.G：
Culture medium main body is：Sucrose 0.1%, peptone 0.1%, gellan gum 0.1%, Mg2+0.02%, balance of water；
The inorganic salts of following content are added in culture medium main body：Diphosphonic acid hydrogen potassium 0.0025%, sodium chloride 0.0125%, ferrous sulfate
0.0005%, sodium molybdate 0.0005%, manganese sulfate 0.0005%, calcium carbonate 0.01%.
3# culture mediums P.S.G：
Culture medium main body is：Sucrose 1%, peptone 2%, gellan gum 1%, Mg2+0.02%, balance of water；In culture medium
The inorganic salts of following content are added in main body：Diphosphonic acid hydrogen potassium 0.0025%, sodium chloride 0.0125%, ferrous sulfate
0.0005%, sodium molybdate 0.0005%, manganese sulfate 0.0005%, calcium carbonate 0.01%.
4# culture mediums P.S.G：
Culture medium main body is：Sucrose 1%, peptone 2%, gellan gum 1%, Mg2+0.05%, balance of water；In culture medium
The inorganic salts of following content are added in main body：Diphosphonic acid hydrogen potassium 0.0025%, sodium chloride 0.0125%, ferrous sulfate
0.0005%, sodium molybdate 0.0005%, manganese sulfate 0.0005%, calcium carbonate 0.01%.
5# culture mediums P.S.G：
Culture medium main body is：Sucrose 1%, peptone 2%, gellan gum 1%, Mg2+0.02%, balance of water.Directly with training
Base main body is supported as 5# culture mediums P.S.G, i.e. without other inorganic salts.
The equal solidifiable of gellan gum in above-mentioned culture medium.
, as experiment three is detected, cultivate and observe within 21 days the straight of bacteria cake growth using with three identical ustilaginoidea virens of experiment
Footpath, calculates its growth rate.
Result such as table 7 below：
Peptone sucrose gellan gum culture medium (P.S.G) of table 7, different formulations
Cultivation results after the formula change of culture medium are learnt, raising sucrose, peptone, the content of gellan gum, or
The growth rate of ustilaginoidea virens can be influenceed without inorganic salts, it follows that in P.S.G culture mediums, sucrose is 1%, peptone
It is 2%, gellan gum is 0.3%, Mg2+It is 0.01%, 0.02%, 0.05%, 0.1%, inorganic salts：Diphosphonic acid hydrogen potassium
0.0025%, sodium chloride 0.0125%, ferrous sulfate 0.0005%, sodium molybdate 0.0005%, manganese sulfate 0.0005%, calcium carbonate
0.01%, this formula is the optimum formula of ustilaginoidea virens growth.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, this hair
It is bright to be not limited to above example, there can also be many deformations.One of ordinary skill in the art can be from present disclosure
The all deformations directly derived or associate, are considered as protection scope of the present invention.
Claims (5)
1. the culture medium of ustilaginoidea virens growth is adapted to, it is characterized in that culture medium main body is into being grouped into by following weight content：
2. the culture medium that suitable ustilaginoidea virens according to claim 1 grows, it is characterized in that：In 1kg culture medium main bodys
Addition diphosphonic acid hydrogen 25 ± 3mg of potassium, 125 ± 10mg of sodium chloride, 5 ± 1mg of ferrous sulfate, 5 ± 1mg of sodium molybdate, manganese sulfate 5 ±
1mg, 100 ± 10mg of calcium carbonate, obtain the culture medium of suitable ustilaginoidea virens growth.
3. the culture medium that suitable ustilaginoidea virens according to claim 1 and 2 grows, it is characterized in that：Mg2+It is magnesium sulfate or sulphur
Sour magnesium hydrate.
4. the culture medium that suitable ustilaginoidea virens according to claim 3 grows, it is characterized in that：In the culture medium main body,
Sucrose is 1%, and peptone is 2%, and gellan gum is 0.3%, Mg2+It is 0.02%.
5. the culture medium that suitable ustilaginoidea virens according to claim 4 grows, it is characterized in that：The water is ultra-pure water.
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| US20140248651A1 (en) * | 2011-08-01 | 2014-09-04 | Dohler Gmbh | Nutrient medium |
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| US20140248651A1 (en) * | 2011-08-01 | 2014-09-04 | Dohler Gmbh | Nutrient medium |
| CN103103136A (en) * | 2013-02-25 | 2013-05-15 | 福建省农业科学院植物保护研究所 | Effective Ustilaginoidea virens separation method |
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| CN115181703A (en) * | 2022-07-18 | 2022-10-14 | 江南大学 | Solid culture medium for rapid growth of micromonospora for sisomicin production |
| CN115181703B (en) * | 2022-07-18 | 2023-10-27 | 江南大学 | Solid medium for fast growth of micromonospora for sisomicin production |
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