CN106905425A - A kind of preparation method of regenerated silk fibroin - Google Patents
A kind of preparation method of regenerated silk fibroin Download PDFInfo
- Publication number
- CN106905425A CN106905425A CN201710248469.0A CN201710248469A CN106905425A CN 106905425 A CN106905425 A CN 106905425A CN 201710248469 A CN201710248469 A CN 201710248469A CN 106905425 A CN106905425 A CN 106905425A
- Authority
- CN
- China
- Prior art keywords
- silk fibroin
- regenerated silk
- fibroin
- salt
- molten
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Insects & Arthropods (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of preparation method of regenerated silk fibroin, specifically by through the discarded silk of thermokalite degumming including through salt is molten, coarse filtration, ultrafiltration, dry including step and obtain；It is that degumming fibroin albumen is put into mixing salt solution that wherein salt is molten, and 10~15min is heated at 85~100 DEG C；The mixing salt solution is the ethanol water of the calcium chloride of 3.5~4.5mol/L, and the ethanol volume accounting wherein in ethanol water is 20~40%.The inventive method prepares the scheme of regenerated silk fibroin compared with prior art, and the dissolved efficiency of fibroin albumen and acquired regenerated silk fibroin dissolubility, product yield reach splendid value simultaneously；The molecular weight distribution of resulting regenerated silk fibroin product is in 35~245kDa.The inventive method technique takes that short, efficiency high, effect are good, contributes to regenerated silk fibroin in the further genralrlization application in the fields such as food.
Description
Technical field
The present invention relates to the preparation of fibroin albumen, more particularly to a kind of preparation method of regenerated silk fibroin.
Background technology
Natural fibroin albumen has antiparallel beta sheet structure, in intermolecular and intramolecular hydrogen bond and Van der Waals force
In the presence of Stability Analysis of Structures, it is therefore water insoluble.Insoluble natural fibroin albumen is changed into soluble regenerated silk fibroin
(regenerated silk fibroin, RSF), can expand its application field；Regenerated silk fibroin and tissue biofacies
Capacitive is high, and Medicated Permeation is strong, and good mechanical performance and degradability, can be applied to biomedicine, medical equipment, tissue
Engineering and delivery system；In terms of food, research shows that regenerated silk fibroin has reducing blood lipid, promotes alcohol metabolism and drop
The effects such as blood sugar, make an addition to and can have additional nutrients in food, promote health；Research also found that regenerated silk fibroin can also change
The processing characteristics of kind food.A series of Japanese early beverage for having been developed that addition regenerated silk fibroins, fruit wine and cake, it is domestic
Having researcher improves the mouthfeel of cake using the good foaming characteristic of regenerated silk fibroin.
Find that prior art is mainly to be studied the application of regenerated silk fibroin through retrieval, and to fibroin albumen
Dissolving further investigate, the preparation process for regenerated silk fibroin does not carry out case study substantially, especially not to again
The preparation method of raw silk fibroin is studied with the relation of yield, efficiency and properties of product.
The content of the invention
It is an object of the invention to provide a kind of preparation method of the regenerated silk fibroin that dissolubility is good, production efficiency is high.
The preparation method of regenerated silk fibroin of the present invention, specifically by through the discarded silk of thermokalite degumming include through salt it is molten,
Coarse filtration, ultrafiltration, drying are obtained in interior step；The salt is molten, is that the degumming fibroin albumen input that will be obtained through thermokalite degumming is mixed
Salting liquid is closed, 10~15min is heated at 85~100 DEG C；The mixing salt solution is preferably using the chlorination of 3.5~4.5mol/L
Ethanol volume accounting in the ethanol water of calcium, wherein ethanol water is 20~40%.The input amount of the mixing salt solution
Can be selected according to general knowledge by one of ordinary skill in the art, it is molten preferably to put into 15ml~20ml salt-mixtures by every gram of degumming fibroin albumen
Liquid.
In above method, preferred ultrafiltration, drying process are：The silk fibroin protein solution obtained after the molten step of salt is through thick
After filter, using 5KDa ultrafiltration desalinations, then it is spray-dried.
The present invention have studied the condition that insoluble fibroin albumen changes to soluble regenerated silk fibroin, with fibroin albumen
The dissolubility of production efficiency and regenerated silk fibroin is index analysis dissolution system, temperature, salinity and concentration of alcohol to silk
The influence of fibroin dissolving, works out a kind of preparation technology of the regenerated silk fibroin that can be applied to food.Experiment shows：Pass through
The preferred fibroin albumen molten technique of salt of the above, the inventive method prepares the scheme of regenerated silk fibroin compared with prior art, silk
The parameter such as the dissolved efficiency of fibroin and acquired regenerated silk fibroin dissolubility, product yield reaches splendid value simultaneously；Institute
The molecular weight distribution of the regenerated silk fibroin product for obtaining in 35~245kDa, suitably as the wall material raw material of microcapsule product.
The inventive method technique takes that short, efficiency high, effect are good, contributes to regenerated silk fibroin in the further genralrlization in the fields such as food
Using.
Specific embodiment
With reference to specific embodiment, comparative example and corresponding experimental data, further to regenerated silk fibroin of the present invention
The concrete technical scheme and effect of preparation method are illustrated.It should be understood that these embodiments be only for explanation the present invention without
For limiting embodiments of the present invention.
The embodiment of regenerated silk fibroin preparation method of the present invention：The preparation of regenerated silk fibroin of the present invention is first will be discarded
Silk thermokalite degumming, then through including salt is molten, coarse filtration, ultrafiltration, the step including drying and obtain powdered regenerated silk fibroin
Product；Wherein thermokalite degumming, coarse filtration, ultrafiltration, drying can refer to and use prior art, and salt is molten using following technique：Will be through heat
The degumming fibroin albumen that alkali degumming is obtained, 15ml mixing salt solutions are put into by every gram of degumming fibroin albumen, are added at 85~100 DEG C
10~15min of heat；The mixing salt solution is the ethanol water of the calcium chloride of 3.5~4.5mol/L, wherein ethanol water
In ethanol volume accounting be 20~40%.It is preferably super through 5KDa after the silk fibroin protein solution obtained after the molten step of salt is through coarse filtration
Filter desalination, is then spray-dried, that is, obtain regenerated silk fibroin powdery product.Experiment is shown, is prepared using this technique
Regenerated silk fibroin, fibroin albumen is completely dissolved time 3~13min of average out to, average regenerated silk fibroin yield up to 94%,
Regenerated silk fibroin dissolubility (NSI) is 91~93%, and the average desalination time is 1.5~2.5 hours, shows the molten of fibroin albumen
Solution efficiency is higher；Acquired regenerated silk fibroin dissolubility is good, regenerated silk fibroin molecular weight distribution in 35~245kDa, after
Continuous experiment has preferably emulsifying capacity and emulsion stability when confirming to be used cooperatively with other raw materials, is suitably produced as microcapsules
The wall material raw material of product.
Table 1 below -3 is respectively implementation comparative example and corresponding the experimental data (note that different process prepares regenerated silk fibroin：
The difference of each embodiment is that the molten step of salt employs different technological parameters in table, and other steps are using institute above
State, identical technique)：
Table 1
Table 2
Table 3
Above experimental data shows, by the preferred molten technique of fibroin albumen salt, using the inventive method preparation regenerated silk
During fibroin, the dissolved efficiency of fibroin albumen and acquired regenerated silk fibroin dissolubility, regenerated silk fibroin yield are simultaneously
Splendid value is reached, and the desalination time is shorter.
Above content is only some better embodiments enumerated to introduce technical solution of the present invention, be not construed as
Limit form of implementation of the invention.The equivalent variations that all one of ordinary skill in the art can make according to technical solution of the present invention with change
Enter, all should belong within the covering scope of technical solution of the present invention.
Claims (3)
1. a kind of preparation method of regenerated silk fibroin, it is characterised in that：It is that will include through salt through the discarded silk of thermokalite degumming
Molten, coarse filtration, ultrafiltration, drying are obtained in interior step；
The salt is molten, is the degumming fibroin albumen input mixing salt solution that will be obtained through thermokalite degumming, is heated at 85~100 DEG C
10~15min；The mixing salt solution is the ethanol water of the calcium chloride of 3.5~4.5mol/L, wherein in ethanol water
Ethanol volume accounting be 20~40%.
2. the preparation method of regenerated silk fibroin according to claim 1, it is characterised in that：Obtained after the molten step of salt
Silk fibroin protein solution through coarse filtration after, using 5KDa ultrafiltration desalinations, be then spray-dried.
3. the preparation method of regenerated silk fibroin according to claim 1 and 2, it is characterised in that institute in the molten step of salt
The usage amount for stating mixing salt solution is：Every gram of degumming fibroin albumen puts into 15~20ml mixing salt solutions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710248469.0A CN106905425A (en) | 2017-04-14 | 2017-04-14 | A kind of preparation method of regenerated silk fibroin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710248469.0A CN106905425A (en) | 2017-04-14 | 2017-04-14 | A kind of preparation method of regenerated silk fibroin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106905425A true CN106905425A (en) | 2017-06-30 |
Family
ID=59210618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710248469.0A Pending CN106905425A (en) | 2017-04-14 | 2017-04-14 | A kind of preparation method of regenerated silk fibroin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106905425A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775465A (en) * | 2012-08-01 | 2012-11-14 | 中国人民解放军第三军医大学 | Method for preparing regenerative silk fibroin and product and application thereof |
| CN103768654A (en) * | 2012-10-18 | 2014-05-07 | 上海纳米技术及应用国家工程研究中心有限公司 | Water-soluble fibroin powder and preparation method thereof |
| CN105534869A (en) * | 2016-01-30 | 2016-05-04 | 常州市鼎升环保科技有限公司 | Preparation method for anti-ultraviolet silk fibroin |
| CN106046135A (en) * | 2016-08-08 | 2016-10-26 | 南通大学 | Silk fibroins with different degradation rates and use thereof |
| CN106243210A (en) * | 2016-08-12 | 2016-12-21 | 西南大学 | A kind of less salt anhydrous solvent system dissolves the method for natural silk |
-
2017
- 2017-04-14 CN CN201710248469.0A patent/CN106905425A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775465A (en) * | 2012-08-01 | 2012-11-14 | 中国人民解放军第三军医大学 | Method for preparing regenerative silk fibroin and product and application thereof |
| CN103768654A (en) * | 2012-10-18 | 2014-05-07 | 上海纳米技术及应用国家工程研究中心有限公司 | Water-soluble fibroin powder and preparation method thereof |
| CN105534869A (en) * | 2016-01-30 | 2016-05-04 | 常州市鼎升环保科技有限公司 | Preparation method for anti-ultraviolet silk fibroin |
| CN106046135A (en) * | 2016-08-08 | 2016-10-26 | 南通大学 | Silk fibroins with different degradation rates and use thereof |
| CN106243210A (en) * | 2016-08-12 | 2016-12-21 | 西南大学 | A kind of less salt anhydrous solvent system dissolves the method for natural silk |
Non-Patent Citations (2)
| Title |
|---|
| 刘晨: "离心纺不同分子量再生丝素蛋白的可纺性研究", 《中国优秀硕士学位论文全文数据库》 * |
| 苏州丝绸工学院: "《高等纺织院校教材 制丝化学》", 31 October 1979 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Characterization and functional properties of mango peel pectin extracted by ultrasound assisted citric acid | |
| Piccinno et al. | Life cycle assessment of a new technology to extract, functionalize and orient cellulose nanofibers from food waste | |
| CN102766224B (en) | Production technology of low-temperature instant-dissolving agar | |
| CN104086674B (en) | A kind of technique preparing Enoxaparin Sodium | |
| JP2016512702A (en) | Method for extracting protein from oil seeds | |
| CN103238680B (en) | Walnut oil microcapsule and preparation method thereof | |
| CN102327238A (en) | Levocarnitine composition for injection and preparation method of levocarnitine composition | |
| CN108064976A (en) | A kind of method that polysaccharide emulsion gel is prepared using Pickering lotions | |
| CN102258488B (en) | Clindamycin phosphate composition for injection and preparation method thereof | |
| CN101700225B (en) | Fat emulsion injection and production method thereof | |
| CN102718990A (en) | Method for preparing high-stable sweet potato pectin emulsion | |
| CN109259033A (en) | A kind of coconut slurry powder and preparation method thereof | |
| CN106905425A (en) | A kind of preparation method of regenerated silk fibroin | |
| CN103520103A (en) | Vitamin E nano-emulsion and preparation method thereof | |
| CN106262764A (en) | Natural clarifying agent and application thereof | |
| CN102775510B (en) | Preparation technique of low-temperature instant carrageenan | |
| CN106309509B (en) | Inonotus obliquus polyphenol complex compound and preparation method thereof | |
| CN104592340A (en) | Glycyrrhizinic acid monoammonium salt fine product preparation method | |
| CN106967494A (en) | A kind of method for extracting coconut oil | |
| CN104327935A (en) | Egg oil and preparation method and application thereof | |
| Zhao et al. | Effects of pre-emulsion prepared using sucrose esters with different hydrophile-lipophile balances on characteristics of soy protein isolate emulsion films | |
| CN104327282B (en) | A kind of multiple aggregation silk fibroin hydrogel and preparation method thereof | |
| EP2417859B1 (en) | Concentrated protein products and methods for producing same | |
| CN105106106A (en) | Preparation method of vaccarin ointment | |
| CN104844809B (en) | A kind of preparation method of tamarind seed polysaccharide gel |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170630 |