CN106896100A - The quick decision-making system of antibiotic residue in a kind of livestock products - Google Patents
The quick decision-making system of antibiotic residue in a kind of livestock products Download PDFInfo
- Publication number
- CN106896100A CN106896100A CN201510949003.4A CN201510949003A CN106896100A CN 106896100 A CN106896100 A CN 106896100A CN 201510949003 A CN201510949003 A CN 201510949003A CN 106896100 A CN106896100 A CN 106896100A
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- nitrite ion
- antibiotic
- small chamber
- determination module
- reaction small
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- 230000003115 biocidal effect Effects 0.000 title claims abstract description 59
- 244000144972 livestock Species 0.000 title claims abstract description 19
- 238000006243 chemical reaction Methods 0.000 claims abstract description 53
- 229940005654 nitrite ion Drugs 0.000 claims abstract description 45
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims abstract description 44
- 238000001514 detection method Methods 0.000 claims abstract description 36
- 238000012360 testing method Methods 0.000 claims abstract description 36
- 241000894007 species Species 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 24
- 230000004044 response Effects 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 241000238097 Callinectes sapidus Species 0.000 claims description 3
- 238000002845 discoloration Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 239000003242 anti bacterial agent Substances 0.000 abstract description 6
- 229940088710 antibiotic agent Drugs 0.000 abstract description 6
- 150000001875 compounds Chemical class 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 description 4
- 235000013365 dairy product Nutrition 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 206010002199 Anaphylactic shock Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 230000002223 anti-pathogen Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of quick decision-making system of antibiotic residue in livestock products, including：Sample tap, reaction small chamber, nitrite ion locker room, detection mouth；Sample tap is sampled to detected sample, and nitrite ion and the testing sample are sufficiently mixed in the reaction small chamber and flow to the detection mouth；The detection mouth is provided with image acquisition device and determination module, and image acquisition device starts species and concentration that determination module judges antibiotic in the testing sample after nitrite ion and the testing sample occur metachromasia.Present invention employs the principle matched two-by-two between different antibiotic and different color-developing compounds, the identification Antibiotics and concentration of full intelligence quickly and accurately realize the detection of antibiotic residue.
Description
Technical field
The present invention relates to antibiotics leftover detection technical field, the quick of antibiotic residue is sentenced in specially a kind of livestock products
Determine system.
Background technology
Antibiotic（antibiotics）It is by microorganism（Including bacterium, fungi, actinomyces）Or high animals and plants are in life
The produced class secondary metabolite with antipathogen or other activity during work, can disturb other living cells to send out
The chemical substance of function is educated, now conventional antibiosis have extract and chemically synthesis or hemizygous in microbial culture medium
Into compound.Antibiotic is widely used as antibiotic and feed addictive, is likely to result in antibiotic animal derived
Residual in food.For dairy products such as milk, antibiotic residue can not only influence the quality of dairy products, such as have a strong impact on fermentation
The production of dairy products, reduces yield and destruction later product local flavor, and economic loss is caused to the producer；In addition, antibiotic
Residual can cause greatly harm to human body, such as cause the allergic reaction of human body（Such as：Fash, anaphylactic shock etc.）, suppress intestines
The growth of normal sensitivity flora, makes pathogenic bacteria, candida albicans largely breed and cause whole body or local infection, and can lead in road
Cause human body to develop immunity to drugs antibiotic, immeasurable harm is brought to clinical treatment.At present, milk, honey, poultry, water
Antibiotic residue in product has caused the great attention of consumer, therefore, the residue detection for strengthening antibiotic is particularly important.
The current detection method on antibiotic residue is mainly traditional microorganism detection method, but traditional micro- life
Long the time required to object detecting method, color status judge, by unaided eye discrimination, to be also easy to produce error.Antibiosis can not accurately be realized
The detection of element residual.
The content of the invention
It is an object of the invention to provide a kind of quick decision-making system of antibiotic residue in livestock products, to solve the above-mentioned back of the body
The problem proposed in scape technology.
To achieve the above object, the present invention provides following technical scheme：The quick of antibiotic residue is sentenced in a kind of livestock products
Determine system, including：
Sample tap, reaction small chamber, nitrite ion locker room, detection mouth；
The sample tap and the nitrite ion locker room are connected to the both sides of the reaction small chamber, the reaction small chamber and institute
The side of detection mouth the 3rd is stated to be connected；
The sample tap is sampled to detected sample, and the nitrite ion and the testing sample fill in the reaction small chamber
Point mixing, it is described to be sufficiently mixed rear liquid and flow to the detection mouth；The detection mouth is provided with image acquisition device and determination module,
Whether described image collector is used to judge the nitrite ion and the testing sample in reaction small chamber generation metachromasia,
If generation metachromasia, start the determination module, the determination module is used to judging antibiotic in the testing sample
Species and concentration.
Further, described image collector is specifically for the nitrite ion in the reaction small chamber and described treating test sample
The reacted liquid of product carries out the collection of red, green and blue component, and then draws tone, color saturation and brightness letter
Breath；
Data memory module is additionally provided with described image collector, the letter of the nitrite ion that is stored with the data memory module
Breath, the information of the antibiotic matched with nitrite ion, and the nitrite ion and the antibiotic matched with nitrite ion become
Tone, color saturation and monochrome information after colour response；
Described image collector by collection after the reaction after the tone of liquid, color saturation and monochrome information and database
Tone, color saturation and monochrome information after the metachromasia of middle storage are contrasted, if unanimously, confirming to occur discoloration instead
Should, start the determination module, the determination module by described image collector by collection after the reaction after liquid color
Adjust, color saturation and monochrome information are converted into digital threshold.
Further, after described image collector is to the nitrite ion in the reaction small chamber and testing sample reaction
Liquid carry out the collection of red, green and blue component, and then draw tone, color saturation and monochrome information, specific bag
Include：
Red, green and blue point are carried out to the nitrite ion in the reaction small chamber and the reacted liquid of the testing sample
The collection of amount；
Brightness is calculated according to formula one
（1）；
Wherein R is red component, and G is green component, and B is blue component；
Color saturation is calculated according to formula two
（2）；
Tone is calculated according to formula three or formula four：
（3）；
（4）.
Further, the determination module is additionally operable to, by antibiotic concentration in the brightness judgement testing sample.
Further, also include：Warning device；
The warning device is connected with the determination module, and the digital threshold of liquid exceeds institute after reaction after collection
When stating predetermined threshold value in determination module, the determination module controls the warning device to send alarm.
Further, the warning device is buzzer.
Further, the warning device is LED light.
Further, the first pump, the institute of nitrite ion locker room are provided between the sample tap and the reaction small chamber
State and be provided with first switch between reaction small chamber.
Further, it is provided with second switch between the reaction small chamber and the detection mouth.
Compared with prior art the beneficial effects of the invention are as follows：The quick judgement of antibiotic residue in livestock products of the invention
System, is sufficiently mixed by making the nitrite ion and the testing sample in the reaction small chamber, described to be sufficiently mixed rear liquid
Body flow to the detection mouth；The detection mouth detects whether the nitrite ion and the testing sample occur in the reaction small chamber
Metachromasia, if there is metachromasia, judges the species and concentration of antibiotic in the testing sample.
Present invention employs the principle matched two-by-two between different antibiotic and different color-developing compounds, to current world city
The all antibiotic generally used on field have carried out the basic research work of minimum developer amount, as long as using antibiotic
Animal product, as long as reaching the minimum residue criterion dosage of detection, testing staff just can in real time obtain result of determination.The knowledge of full intelligence
Other Antibiotics and concentration, quickly and accurately realize the detection of antibiotic residue.
Brief description of the drawings
Fig. 1 is the structural representation of the quick decision-making system of antibiotic residue in livestock products of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
The structural representation of the quick decision-making system of antibiotic residue, refers in the livestock products that Fig. 1 is provided for the present invention
Fig. 1, the present invention provides a kind of quick decision-making system of antibiotic residue in livestock products, including：
Sample tap 1, reaction small chamber 3, nitrite ion locker room 6, detection mouth 5；6 points of the sample tap 1 and the nitrite ion locker room
The both sides of the reaction small chamber 3 are not connected to, and the reaction small chamber 3 is connected with the side of the detection mouth 3 the 3rd；The sample tap
1 pair of detected sample is sampled, and the nitrite ion and the testing sample are sufficiently mixed in the reaction small chamber 3, described
It is sufficiently mixed rear liquid and flow to the detection mouth 5；The detection mouth 5 is provided with image acquisition device and determination module, described image
Collector is used to judge whether the nitrite ion and the testing sample occur metachromasia in the reaction small chamber, if becoming
Colour response, then start the determination module, the determination module be used to judge antibiotic in the testing sample species and
Concentration.
Specifically, the sample tap 1 is connected by first connecting portion 2 with the reaction small chamber 3, the nitrite ion storage
Room 6 is connected by second connecting portion 61 with the reaction small chamber 3, and the detection mouth 3 passes through the 3rd connecting portion 4 and the reaction
Cell 3 is connected.
Further, the first pump 21, the nitrite ion locker room are provided between the sample tap 1 and the reaction small chamber
First switch 62 is provided between the reaction small chamber.
Specifically, the first pump 21 is arranged in first connecting portion 2, for controlling sample tap 1 to pump into detected sample, first
Switch 62 is arranged in second connecting portion 61, in nitrite ion is flowed into reaction small chamber 3.
Further, second switch 41 is provided between the reaction small chamber 3 and the detection mouth 5.
Specifically, second switch 41 is arranged on the 3rd connecting portion 4, for making the testing sample after being sufficiently mixed and reaction
Liquid flows into detection mouth 5.
The quick decision-making system of antibiotic residue of the invention, by making the nitrite ion and the testing sample described
It is sufficiently mixed in reaction small chamber, it is described to be sufficiently mixed rear liquid and flow to the detection mouth；The detection mouth detects the nitrite ion
With the testing sample whether in reaction small chamber generation metachromasia, if there is metachromasia, test sample is treated described in judgement
The species and concentration of antibiotic in product.The principle matched two-by-two between different antibiotic and different color-developing compounds is employed,
All antibiotic to generally being used on current international market have carried out the basic research work of minimum developer amount, as long as making
The animal product of used antibiotic, as long as reaching the minimum residue criterion dosage of detection, testing staff just can obtain judging knot in real time
Really.The identification Antibiotics and concentration of full intelligence, quickly and accurately realize the detection of antibiotic residue.
In the present embodiment, described image collector is reacted the nitrite ion in the reaction small chamber and the testing sample
Liquid afterwards carries out the collection of red, green and blue component, and then draws tone, color saturation and monochrome information；
Data memory module is additionally provided with described image collector, the letter of the nitrite ion that is stored with the data memory module
Breath, the information of the antibiotic matched with nitrite ion, and the nitrite ion and the antibiotic matched with nitrite ion become
Tone, color saturation and monochrome information after colour response；
Described image collector by collection after the reaction after the tone of liquid, color saturation and monochrome information and database
Tone, color saturation and monochrome information after the metachromasia of middle storage are contrasted, if unanimously, confirming to occur discoloration instead
Should, start the determination module, the determination module by described image collector by collection after the reaction after liquid color
Adjust, color saturation and monochrome information are converted into digital threshold.
In the present embodiment, described image collector is reacted the nitrite ion in the reaction small chamber and the testing sample
Liquid afterwards carries out the collection of red, green and blue component, and then draws tone, color saturation and monochrome information；Specifically
Including：
Red, green and blue point are carried out to the nitrite ion in the reaction small chamber and the reacted liquid of the testing sample
The collection of amount；
Brightness is calculated according to formula one
（1）；
, wherein R is red component, and G is green component, and B is blue component；
Color saturation is calculated according to formula two
（2）；
Tone is calculated according to formula three or formula four：
（3）；
（4）.
When B is less than or equal to G, tone can be calculated by publicity three, when B is more than G, can be counted by formula four
Calculation obtains tone.
For example, three primary color values for setting certain pixel are R=100, G=20, B=200.It is calculated：Brightness L=(100+20+
200)/3=107, brightness is medium（0 is completely black, and 255 is most bright）；Color saturation S=1-20/107=81.3%, color is deeper
（100% is most deep）；H=257 ° of tone, it is purple partially blue.By brightness, color saturation and tone, it can be determined that go out color, and then sentence
Break the Antibiotics in testing sample.
In the present embodiment, the determination module is additionally operable to, and antibiotic is dense in judging the testing sample by the brightness
Degree.Accordingly, by the antibiotic concentration output matched with brightness concentration, testing staff is made to observe antibiosis in detected sample
Plain concentration.
In the present embodiment, also include, warning device；The warning device is connected with the determination module, after collection
The reaction after liquid digital threshold in the determination module during predetermined threshold value, the determination module controls the report
Alarm device sends alarm.
The warning device can be buzzer, and the digital threshold of liquid is sentenced beyond described after reaction after collection
In cover half block during predetermined threshold value, buzzer buzzing.The warning device can also be LED light, described anti-after collection
The digital threshold of liquid is beyond during predetermined threshold value, LED light flashes in the determination module after answering.As long as it is minimum to reach detection
Residue criterion dosage, detection means and sends alarm, and testing staff just can in real time obtain result of determination.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (9)
1. in a kind of livestock products antibiotic residue quick decision-making system, it is characterised in that including：
Sample tap, reaction small chamber, nitrite ion locker room, detection mouth；
The sample tap and the nitrite ion locker room are connected to the both sides of the reaction small chamber, the reaction small chamber and institute
The side of detection mouth the 3rd is stated to be connected；
The sample tap is sampled to detected sample, and the nitrite ion and the testing sample fill in the reaction small chamber
Point mixing, it is described to be sufficiently mixed rear liquid and flow to the detection mouth；The detection mouth is provided with image acquisition device and determination module,
Whether described image collector is used to judge the nitrite ion and the testing sample in reaction small chamber generation metachromasia,
If generation metachromasia, start the determination module, the determination module is used to judging antibiotic in the testing sample
Species and concentration.
2. in livestock products according to claim 1 antibiotic residue quick decision-making system, it is characterised in that described image
Collector is red, green specifically for being carried out to the nitrite ion in the reaction small chamber and the reacted liquid of the testing sample
The collection of color and blue component, and then draw tone, color saturation and monochrome information；
Data memory module is additionally provided with described image collector, the letter of the nitrite ion that is stored with the data memory module
Breath, the information of the antibiotic matched with nitrite ion, and the nitrite ion and the antibiotic matched with nitrite ion become
Tone, color saturation and monochrome information after colour response；
Described image collector by collection after the reaction after the tone of liquid, color saturation and monochrome information and database
Tone, color saturation and monochrome information after the metachromasia of middle storage are contrasted, if unanimously, confirming to occur discoloration instead
Should, start the determination module, the determination module by described image collector by collection after the reaction after liquid color
Adjust, color saturation and monochrome information are converted into digital threshold.
3. in livestock products according to claim 2 antibiotic residue quick decision-making system, it is characterised in that
Described image collector carries out red to the nitrite ion in the reaction small chamber and the reacted liquid of the testing sample
The collection of color, green and blue component, and then tone, color saturation and monochrome information are drawn, specifically include：
Red, green and blue point are carried out to the nitrite ion in the reaction small chamber and the reacted liquid of the testing sample
The collection of amount；
Brightness is calculated according to formula one
（1）；
Wherein R is red component, and G is green component, and B is blue component；
Color saturation is calculated according to formula two
（2）；
Tone is calculated according to formula three or formula four：
（3）；
（4）.
4. in livestock products according to claim 2 antibiotic residue quick decision-making system, it is characterised in that
The determination module is additionally operable to, by antibiotic concentration in the brightness judgement testing sample.
5. in livestock products according to claim 1 antibiotic residue quick decision-making system, it is characterised in that
Also include：Warning device；
The warning device is connected with the determination module, and the digital threshold of liquid exceeds institute after reaction after collection
When stating predetermined threshold value in determination module, the determination module controls the warning device to send alarm.
6. in livestock products according to claim 5 antibiotic residue quick decision-making system, it is characterised in that
The warning device is buzzer.
7. in livestock products according to claim 5 antibiotic residue quick decision-making system, it is characterised in that
The warning device is LED light.
8. in livestock products according to claim 1 antibiotic residue quick decision-making system, it is characterised in that
The first pump is provided between the sample tap and the reaction small chamber, between reaction small chamber described in the nitrite ion locker room
It is provided with first switch.
9. in livestock products according to claim 1 antibiotic residue quick decision-making system, it is characterised in that
Second switch is provided between the reaction small chamber and the detection mouth.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510949003.4A CN106896100A (en) | 2015-12-18 | 2015-12-18 | The quick decision-making system of antibiotic residue in a kind of livestock products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510949003.4A CN106896100A (en) | 2015-12-18 | 2015-12-18 | The quick decision-making system of antibiotic residue in a kind of livestock products |
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| Publication Number | Publication Date |
|---|---|
| CN106896100A true CN106896100A (en) | 2017-06-27 |
Family
ID=59188928
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510949003.4A Pending CN106896100A (en) | 2015-12-18 | 2015-12-18 | The quick decision-making system of antibiotic residue in a kind of livestock products |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109030480A (en) * | 2018-08-16 | 2018-12-18 | 湖南友哲科技有限公司 | Sample analysis method, device, readable storage medium storing program for executing and computer equipment |
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2015
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| US20050014281A1 (en) * | 2001-10-15 | 2005-01-20 | Langeveld Pieter Cornelis | Apparatus and method for detecting undesired residues in a sample |
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Application publication date: 20170627 |