CN106893684A - A kind of restructuring yeast strains and its application - Google Patents
A kind of restructuring yeast strains and its application Download PDFInfo
- Publication number
- CN106893684A CN106893684A CN201710090086.5A CN201710090086A CN106893684A CN 106893684 A CN106893684 A CN 106893684A CN 201710090086 A CN201710090086 A CN 201710090086A CN 106893684 A CN106893684 A CN 106893684A
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- yeast
- promoters
- lycopene
- ald6
- restructuring
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
Abstract
The present invention relates to gene engineering technology field, a kind of restructuring yeast strains and its application are disclosed.Restructuring yeast strains of the present invention are lowered for the yeast strain of fermenting and producing lycopene, and its ALD6 promoters expression intensity.The present invention is by the further investigation to yeast strain ALD6 promoters, it was found that building the recombinant bacterial strain of production lycopene by way of all knocking out or lowering ALD6 promoter expression intensities with low expression intensity promoter replacement etc., it is capable of the yield of lycopene of the notable production bacterial strain, the more excellent recombination engineering of production capacity can be constructed as the optimization means of Yeast engineering bacteria synthesis path from now on.
Description
Technical field
The present invention relates to gene engineering technology field, more particularly to a kind of restructuring yeast strains and its application.
Background technology
Worldwide, with economic level and the raising to health demand, the security of food, trophism and function
Property receive more and more attention, therefore functional nutrient chemicals has turned into development trend, represents the new of contemporary food development
Trend, with wide market prospects.Lycopene is a kind of fat-soluble natural food colour, and class is belonged in chemical constitution recklessly
Radish element, possesses extremely strong oxidation resistance, is the focus that functional food composition is studied in the world in recent years.
The preparation of lycopene relies primarily on plant extract, chemical synthesis and Microbe synthesis, and first two method respectively has it
The deficiency of itself, Microbe synthesis are then with low cost, high yield and Product Safety, it is considered to be most promising method.Mesh
Before, in the research of Microbe synthesis lycopene, the Host Strains for being used focus primarily upon Escherichia coli and saccharomyces cerevisiae.Make
, used as generally acknowledged safe mode microorganism, compared to Escherichia coli, its thalline vitamin, protein content are high, can make for brewer yeast
Edible, medicinal and fodder yeast；Compared to trispore Bruce mould, its growth cycle is shorter and is more easy to culture.Therefore, realization kind
High yield of the Lycopene in saccharomyces cerevisiae will represent great competitiveness in carotenoid industrialization.
Adaptation between saccharomyces cerevisiae and lycopene synthesis path is the Main way of current research.One side heterologous organisms
Synthesis path metabolic flux in itself determines the yield of target product, it is therefore desirable to optimizes heterologous path, including optimizes heterologous base
The expression of cause, the screening of gene source, the supply of intracellular precursor substance etc.；On the other hand from the intrinsic metabolism of host cell and tune
Control system can also influence the production capacity of target organism synthesis path, and this is accomplished by that chassis cell is carried out to go deep into the excellent of system
Change.
In saccharomyces cerevisiae, Gene A LD6 (being controlled by ALD6 promoters) encoding glyoxylate dehydrogenase, in activated cell matter
Acetaldehyde generates acetic acid.The expression of Gene A LD6 can directly affect the accumulation of acetic acid in cell, but not yet have any gene
The report of yield of lycopene can be influenceed.
The content of the invention
In view of this, it is an object of the invention to provide a kind of restructuring yeast strains so that the recombinant bacterial strain is for sending out
Yield can be improved during ferment production lycopene, in may apply to related lycopene preparation field.
For achieving the above object, the present invention provides following technical scheme：
A kind of restructuring yeast strains, the bacterial strain is the yeast strain of fermenting and producing lycopene, and its ALD6 promoter
Expression intensity is lowered.
In terms of the research of current yeast strain ALD6 promoter functions is limited to Acetic Acid Accumulation, and otherwise function
There is not relevant report, the present invention is directed to current present situation, ALD6 promoters are studied from many aspects, finds ALD6 promoters
Expression intensity lowers the yield of the yeast strain that can increase production lycopene.
The ALD6 promoters expression intensity of the present invention is adjusted to by under and directly knock out ALD6 promoters or using expression
Intensity is replaced less than other promoters of ALD6 promoters.In the present invention, expression intensity is opened less than other of ALD6 promoters
Mover selection is URA3 promoters, nucleotide sequence such as SEQ ID NO:Shown in 2.
Preferably, yeast strain of the present invention is saccharomyces cerevisiae, CEN.PK series saccharomyces cerevisiaes are may be selected from, such as made wine
Yeast CEN.PK2-1C or saccharomyces cerevisiae CEN.PK2-1D or saccharomyces cerevisiae CEN.PK2, also selected from BY series saccharomyces cerevisiae, such as
BY4741 saccharomyces cerevisiaes or BY4742 saccharomyces cerevisiaes or BY4743 saccharomyces cerevisiaes.
Yeast strain does not possess the ability of production lycopene in itself, lacks part enzyme, it is necessary to build foreign gene element
Import and wherein turn into production bacterial strain.Import in the present invention through as follows on yeast autologous recombination and integration to its genome
Genetic fragment：
Yeast trp1 site upstreams homologous sequence, CYC1 terminators, trispore Bruce mould (Blakeslea
Trispora) crtI, GAL10 promoter in source, GAL1 promoters, pantoea agglomerans (Pantoea agglomerans) source
CrtB, PGK1 terminator, the genetic fragment 1 that is sequentially spliced of yeast trp1 sites downstream homologous sequences；
Yeast leu2 site upstreams homologous sequence, LEU2 marks, TDH2 terminators, ACT1 terminators, the HMG-CoA of truncation
What reductase gene tHMGR1, GAL10 promoter, GAL1 promoters, pantoea agglomerans (Pantoea agglomerans) were originated
The genetic fragment 2 that crtE, GPM1 terminator, yeast leu2 sites downstream homologous sequences are sequentially spliced.
The building mode of above-mentioned two genetic fragment is recorded in patent CN105087406A, wherein genetic fragment of the present invention
1 is the genetic fragment 1 in CN105087406A, and genetic fragment of the present invention 2 is the genetic fragment 2 in CN105087406A
On the basis of, the crtE, its nucleotide sequence such as SEQ ID NO originated using pantoea agglomerans (Pantoea agglomerans):1
It is shown.
Additionally, in specific embodiment of the invention, restructuring yeast strains of the present invention use saccharomyces cerevisiae
CEN.PK2-1C, it is quadruple auxotroph (leucine, tryptophan, histidine, uracil) saccharomyces cerevisiae, beneficial to screening just
True bacterial strain, while in order to ensure that saccharomyces cerevisiae does not consume derivant galactolipin, the present invention knocked out gal1 in saccharomyces cerevisiae,
Tri- genes of gal7 and gal10, these above-mentioned changes to yeast are intended merely to facilitate the carrying out of checking test, to final effect
The realization of fruit is without influence.
The shake flask test of lycopene is carried out using recombinant Saccharomyces cerevisiae of the present invention, is as a result shown, in identical kind
Under the premise of Lycopene production bacterial strain, the production bacterium of ALD6 promoters or the promoter replacement using relatively low expression intensity is directly knocked out
Strain, the yield of its lycopene is significantly improved.Therefore, the present invention proposes the restructuring yeast strains and is preparing tomato red
Application in element or the application in product of the production with lycopene as intermediate product.
Meanwhile, the invention provides a kind of method of fermenting and producing lycopene, by restructuring yeast strains of the present invention
It is inoculated into seed culture medium and activates to mid log phase, is then transferred to fermenting and producing lycopene in fermentation medium.
In a specific embodiment, methods described is that restructuring yeast strains of the present invention are inoculated in into 5mL seed cultures
In base, in 30 DEG C, 250rpm culture 14-16h, with initial cell concentration OD600=0.2 transfers in fresh 25mL seed cultures
In base, cultivated to mid log phase under the conditions of 30 DEG C, 250rpm, with initial cell concentration OD600=0.5 is inoculated in respectively
In 50mL fermentation mediums, cultivated under the conditions of 30 DEG C, 250rpm, fermenting and producing lycopene.
Wherein, the seed culture medium preferably comprises 20g/L or 40g/L glucose, 20g/L peptones and 10g/L
Yeast extract；The fermentation medium preferably include 20g/L or 40g/L glucose, 20g/L peptones, 10g/L yeast extracts with
And 10g/L D- galactolipins.
From above technical scheme, the present invention has found to pass through by the further investigation to yeast strain ALD6 promoters
All knock out or build production tomato red in the way of ALD6 promoter expression intensities are lowered in low expression intensity promoter replacement etc.
The recombinant bacterial strain of element, is capable of the yield of lycopene of the notable production bacterial strain, can be used as Yeast engineering bacteria synthesis path from now on
Optimization means, construct the more excellent recombination engineering of production capacity.
Brief description of the drawings
Fig. 1 show control lycopene production bacterial strain, knock out ALD6 promoters lycopene production bacterial strain, with URA3
Promoter replaces yield of lycopene of the lycopene production bacterial strain of ALD6 promoters in 20g/L dextrose culture-mediums；Its
In, ordinate is yield of lycopene, and abscissa is three kinds of bacterial strains numbering in the present invention, is corresponding in turn to from left to right in right
According to lycopene production bacterial strain, knock out the lycopene production bacterial strain of ALD6 promoters, ALD6 startups are replaced with URA3 promoters
The lycopene production bacterial strain of son；
Fig. 2 show control lycopene production bacterial strain, knock out ALD6 promoters lycopene production bacterial strain, with URA3
Promoter replaces yield of lycopene of the lycopene production bacterial strain of ALD6 promoters in 40g/L dextrose culture-mediums；Its
In, ordinate is yield of lycopene, and abscissa is three kinds of bacterial strains numbering in the present invention, is corresponding in turn to from left to right in right
According to lycopene production bacterial strain, knock out the lycopene production bacterial strain of ALD6 promoters, ALD6 startups are replaced with URA3 promoters
The lycopene production bacterial strain of son.
Specific embodiment
The invention discloses a kind of restructuring yeast strains and its application, those skilled in the art can use for reference present disclosure,
It is suitably modified technological parameter realization.In particular, all similar replacements and change comes to those skilled in the art
Say it is it will be apparent that they are considered as being included in the present invention.Bacterial strain of the present invention and application have passed through preferably implementation
Example be described, related personnel substantially can not depart from present invention, spirit and scope to bacterial strain described herein and should
Realized and applied the technology of the present invention with being modified or suitably changing with combining.
Each Genetic elements, homologous sequence in genetic fragment of the present invention 1 and 2 etc. are with the base of Wine brewing yeast strain BY4741
Because group is template, suitable primer is designed and synthesized, expanded by PCR and obtained, in specifically can refer to patent CN105087406A
Record.
With reference to embodiment, the present invention is expanded on further.
Embodiment 1：Restructuring yeast strains of the present invention
1st, lycopene produces the structure of bacterial strain
With saccharomyces cerevisiae CEN.PK2-1C as starting strain, according to the method for embodiment in CN105087406A 1 knock out gal1,
Tri- genes of gal7 and gal10, then build genetic fragment 1 according to the method for embodiment in CN105087406A 3 and embodiment 4
With 2, the crtE originated using pantoea agglomerans (Pantoea agglomerans) when genetic fragment 2 is built, its nucleotides
Sequence such as SEQ ID NO:Shown in 1.
Then genetic fragment 1 and 2 is incorporated into wine by homologous recombination according to the method for embodiment in CN105087406A 7
In brewer yeast, the yeast strain of production lycopene is obtained, be named as SyBE_Sc14C07.
2nd, the structure of restructuring yeast strains of the present invention
On the basis of SyBE_Sc14C07, the knockout technique pair of ypl062w is knocked out with reference to embodiment 1 in CN105087406A
ALD6 promoters are knocked out, and difference is the homologous sequence of upstream and downstream homologous sequence ALD6 promoters used, and module used is
ALD6LHA (upstream homologous sequence)-KanMX-ALD6RHA (downstream homologous sequence), obtains restructuring yeast strains of the present invention,
It is named as SyBE_Sc14C66；
On the basis of SyBE_Sc14C07, ALD6LHA, URA3 promoter (its nucleotide sequence such as SEQ ID NO are built:2
It is shown), the genetic fragment sequentially spliced of KanMX and ALD6RHA, be expressed as ALD6LHA-PURA3- KanMX-ALD6RHA, passes through
Homologous recombination replaces ALD6 promoters with URA3 promoters, obtains restructuring yeast strains of the present invention, is named as SyBE_
Sc14C67。
Embodiment 2：Shake flask fermentation is tested
It is right to test with the tri- plants of bacterial strains of SyBE_Sc14C07, SyBE_Sc14C66, SyBE_Sc14C67 in embodiment 1
As carrying out fermentation test as follows：
By inoculation in 5mL seed culture mediums, in 30 DEG C, 250rpm culture 14-16h, with initial cell concentration
OD600=0.2 transfers in fresh 25mL seed culture mediums, is cultivated to mid log phase under the conditions of 30 DEG C, 250rpm,
With initial cell concentration OD600=0.5 is inoculated in 50mL fermentation mediums respectively, is cultivated under the conditions of 30 DEG C, 250rpm, hair
Ferment produces lycopene.
Wherein, the seed culture medium is 20g/L or 40g/L glucose, 20g/L peptones and 10g/L yeast extracts；
The fermentation medium is 20g/L or 40g/L glucose, 20g/L peptones, 10g/L yeast extracts and 10g/L D- galas
Sugar.
Lycopene detection method：After fermentation 48 hours, the zymotic fluid of two equal portions is taken, 4000g is centrifuged 2min collects thallines,
And wash twice.A copy of it thalline is placed in 80 DEG C of drying to constant weight, calculating dry cell weight of weighing；Another thalline is used to produce
Thing is extracted, and specific method is：With 3N HCl re-suspended cells, it is placed in and 2min is boiled in boiling water bath, immediately after ice bath 3min；Will be broken
Broken cell 12000rpm, 4 DEG C of centrifugation 4min abandon supernatant, and acetone, and the 5min that is vortexed are added after washing 2 times；Finally it is collected by centrifugation
Acetone phase, detects that lycopene Detection wavelength is 471nm with upper ultraviolet liquid phase after 2 μm of membrane filtrations.
Result is shown in Fig. 1 and Fig. 2, it can be clearly seen that, restructuring yeast strains of the present invention are in the yield of lycopene
It is significantly improved, and the producing strain for compareing is relatively low, difference between the two is that ALD6 promoters expression intensity is lowered.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
SEQUENCE LISTING
<110>University Of Tianjin
<120>A kind of restructuring yeast strains and its application
<130> MP1701703
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 924
<212> DNA
<213>Artificial sequence
<400> 1
atggtttctg gttctaaggc tggtgtctca ccacacaggg agattgaggt catgaggcag 60
tctattgacg atcacttggc tggtttgttg cctgagactg actctcagga cattgtctca 120
ttggcaatga gggagggtgt catggctcca ggtaagagga taaggccttt gttgatgttg 180
ttggcagcta gggacttgag gtaccagggt tctatgccta ctttgttgga cttggcttgc 240
gctgtcgaat tgactcacac tgcatcattg atgttggacg acatgccttg catggacaac 300
gcagaattaa ggaggggtca gccaacaaca cacaagaagt tcggtgagtc agtcgcaatt 360
ttggcatcag ttggtttgtt atcaaaggct ttcggattga ttgctgcaac tggtgactta 420
ccaggtgaga ggagggcaca ggctgtcaac gagttgtcta ctgctgtcgg agtccaggga 480
ttggtcttgg gtcagttcag ggacttgaac gacgcagctt tggacaggac tccagacgct 540
atattgtcta caaaccactt aaagacagga attttgttct ctgctatgtt gcagatagtc 600
gctattgcat ctgcttcttc tccatctaca agggagactt tgcacgcttt cgcattggac 660
ttcggtcagg ctttccagtt gttggacgac ttgagggacg atcacccaga gacaggaaag 720
gacaggaaca aggatgcagg taaatcaact ttggtcaaca ggttaggtgc agacgctgct 780
aggcagaagt taagggagca cattgactct gctgacaagc acttgacttt cgcttgccca 840
cagggaggtg ctattaggca gttcatgcac ttgtggttcg gtcaccactt agctgactgg 900
tcacctgtca tgaagatagc ttaa 924
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gagtgcacca taccaccttt tcaattcatc attttttttt tattcttttt tttgatttcg 60
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gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc 180
agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcc 236
Claims (10)
1. a kind of restructuring yeast strains, it is characterised in that the bacterial strain is the yeast strain of fermenting and producing lycopene, and its
ALD6 promoters expression intensity is lowered.
2. restructuring yeast strains according to claim 1, it is characterised in that the ALD6 promoters expression intensity is adjusted to by under
Directly knock out ALD6 promoters or replaced less than other promoters of ALD6 promoters using expression intensity.
3. restructuring yeast strains according to claim 2, it is characterised in that described other promoters are URA3 promoters.
4. restructuring yeast strains according to claim 1, it is characterised in that comprising arriving it through yeast autologous recombination and integration
Following genetic fragment on genome：
Yeast trp1 site upstreams homologous sequence, CYC1 terminators, trispore Bruce mould (Blakeslea trispora) come
CrtI, GAL10 promoter in source, GAL1 promoters, crtB, the PGK1 in pantoea agglomerans (Pantoea agglomerans) source
The genetic fragment 1 that terminator, yeast trp1 sites downstream homologous sequences are sequentially spliced；
Yeast leu2 site upstreams homologous sequence, LEU2 marks, TDH2 terminators, ACT1 terminators, the HMG-CoA reduction of truncation
Enzyme gene tHMGR1, GAL10 promoter, GAL1 promoters, pantoea agglomerans (Pantoea agglomerans) source crtE,
The genetic fragment 2 that GPM1 terminators, yeast leu2 sites downstream homologous sequences are sequentially spliced.
5. restructuring yeast strains according to claim 1, it is characterised in that knock out gal1, gal7 and gal10 gene.
6. application of the restructuring yeast strains described in claim 1-5 any one in lycopene is prepared or in production with tomato
Red pigment is the application in the product of intermediate product.
7. a kind of method of fermenting and producing lycopene, it is characterised in that by recombination yeast described in claim 1-5 any one
Then inoculation is transferred to fermenting and producing tomato red in fermentation medium to activating to mid log phase in seed culture medium
Element.
8. method according to claim 7, it is characterised in that by restructuring yeast strains described in claim 1-5 any one
It is inoculated in 5mL seed culture mediums, in 30 DEG C, 250rpm culture 14-16h, with initial cell concentration OD600=0.2 transfers in new
In fresh 25mL seed culture mediums, cultivated to mid log phase under the conditions of 30 DEG C, 250rpm, with initial cell concentration OD600
=0.5 is inoculated in 50mL fermentation mediums respectively, is cultivated under the conditions of 30 DEG C, 250rpm, fermenting and producing lycopene.
9. the fermentation process according to claim 7 or 8, it is characterised in that the seed culture medium includes 20g/L or 40g/L
Glucose, 20g/L peptones and 10g/L yeast extracts.
10. the fermentation process according to claim 7 or 8, it is characterised in that the fermentation medium includes 20g/L or 40g/L
Glucose, 20g/L peptones, 10g/L yeast extracts and 10g/L D- galactolipins.
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| CN201710090086.5A CN106893684A (en) | 2017-02-20 | 2017-02-20 | A kind of restructuring yeast strains and its application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108373980A (en) * | 2018-04-09 | 2018-08-07 | 石河子大学 | A kind of S. cervisiae, its construction method and its application in fermentation prepares lycopene |
| CN109943493A (en) * | 2019-04-17 | 2019-06-28 | 天津大学 | Realize the mutant strain and its construction method of general enzymatic functional diversity |
| CN109943492A (en) * | 2019-04-03 | 2019-06-28 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of restructuring yeast strains and its application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087406A (en) * | 2015-07-22 | 2015-11-25 | 天津大学 | Recombinant yeast strain as well as construction method and application thereof |
| CN105420134A (en) * | 2015-12-25 | 2016-03-23 | 天津大学 | Recombinant yeast strain, and construction method and application thereof |
| CN105441473A (en) * | 2014-08-18 | 2016-03-30 | 中粮营养健康研究院有限公司 | Glutamic acid producing strain and preparation method thereof |
| CN105779319A (en) * | 2016-03-23 | 2016-07-20 | 天津大学 | Recombinant yeast strain, and building method and application thereof |
-
2017
- 2017-02-20 CN CN201710090086.5A patent/CN106893684A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441473A (en) * | 2014-08-18 | 2016-03-30 | 中粮营养健康研究院有限公司 | Glutamic acid producing strain and preparation method thereof |
| CN105087406A (en) * | 2015-07-22 | 2015-11-25 | 天津大学 | Recombinant yeast strain as well as construction method and application thereof |
| CN105420134A (en) * | 2015-12-25 | 2016-03-23 | 天津大学 | Recombinant yeast strain, and construction method and application thereof |
| CN105779319A (en) * | 2016-03-23 | 2016-07-20 | 天津大学 | Recombinant yeast strain, and building method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| CHRISTOPHER J. WALKEY等: "The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae", 《POLS ONE》 * |
| 陈艳: "高产番茄红素酿酒酵母的设计构建与发酵过程优化", 《中国博士学位论文全文数据库》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108373980A (en) * | 2018-04-09 | 2018-08-07 | 石河子大学 | A kind of S. cervisiae, its construction method and its application in fermentation prepares lycopene |
| CN109943492A (en) * | 2019-04-03 | 2019-06-28 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of restructuring yeast strains and its application |
| CN109943492B (en) * | 2019-04-03 | 2022-08-16 | 广东省微生物研究所（广东省微生物分析检测中心） | Recombinant yeast strain and application thereof |
| CN109943493A (en) * | 2019-04-17 | 2019-06-28 | 天津大学 | Realize the mutant strain and its construction method of general enzymatic functional diversity |
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