CN106888965A - The method for building up of Zhejiang roxburgh anoectochilus terminal bud tissue-culturing quick-propagation system - Google Patents
The method for building up of Zhejiang roxburgh anoectochilus terminal bud tissue-culturing quick-propagation system Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention discloses a kind of method for building up of Zhejiang roxburgh anoectochilus terminal bud tissue-culturing quick-propagation system, including protocorms induce, breed, break up and take root step.In liquid medium within, the preferred condition of culture of protocorms induction is TDZ 0.6mg/L+NAA 0.4mg/L, and inductivity is 62.5%；In solid medium, the preferred condition of culture of protocorms induction is TDZ 0.6mg/L+ NAA 0.2mg/L+6 BA 2.0mg/L, and inductivity is 59.3%.The preferred condition of culture of protocorms propagation is the mg/L+6 BA 1.0mg/L+NAA 0.2mg/L of S 3307 1.5, and growth coefficient is 8.4, and wherein S 3307 is the Main Factors of protocorms propagation.The optimum condition of protocorms differentiation is 6 BA 0.5mg/L+NAA 0.1mg/L, and differentiation rate is 87.3%.The preferred condition of culture of plant rooting induction is 1/2 MS+NAA 1.0mg/L+IBA 1.0mg/L, and rooting rate is up to 100%.
Description
Technical field
It is especially a kind of to be related to the foundation side of Zhejiang roxburgh anoectochilus terminal bud tissue culturing system the invention belongs to plant tissue culture field
Method.
Background technology
Zhejiang roxburgh anoectochilus terminal bud（Anoectochilus zhejiangensis）For the perennial rare draft of orchid family Anoectochilus Blume is planted
Thing, is mainly distributed on the ground such as Zhejiang, Fujian, as a kind of precious Chinese herbal medicine, with wide exploitation prospect.Zhejiang gold thread
The biological characteristics and pharmacological activity of lotus are close with base plant roxburgh anoectochilus terminal bud is belonged to together, relatively stricter to growth conditions requirement, natural
Germination rate is less than one thousandth, and the natural propagation time is long and breeding potential is not high, along with a large amount of excavations artificial in recent years, causes
Zhejiang roxburgh anoectochilus terminal bud wild resource is increasingly exhausted, therefore protects wild Zhejiang roxburgh anoectochilus terminal bud to arrive very urgent stage.
Plant Tissue Breeding is the effective technology means for protecting endangered plants, can in a short time breed a large amount of newborn plants
Strain.In tissue cultures, the screening of culture medium will directly influence the success or not of tissue cultures, the concentration proportioning of plant hormone
The fast numerous efficiency of plant will be directly influenced.Therefore success or not of the selection of culture medium and hormone combination to tissue cultures has
Important function.
So far about roxburgh anoectochilus terminal bud base plant, such as quick brood body of TaiWan, China roxburgh anoectochilus terminal bud, roxburgh anoectochilus terminal bud, Anoectochilus roxburghii
The research of system has been reported [foundation of Wang Yong roxburgh anoectochilus terminal bud tissue cultures new system and optimization north gardening, 2010,13: 178-
179；Chen Yong is fast, woods wholeheartedly, the group culturation rapid propagating technology modern horticulturals of the such as Zou Hui Anoectochilus roxburghiis and TaiWan, China roxburgh anoectochilus terminal bud,
2008,10:9-12.], and find that the difference of the rapid propagation system between different base plants is larger, but on Zhejiang roxburgh anoectochilus terminal bud
Research in terms of tissue cultures still belongs to blank.
The content of the invention
In order to overcome the deficiencies in the prior art, this application provides a kind of Zhejiang roxburgh anoectochilus terminal bud tissue-culturing quick-propagation system
Method for building up.
A kind of method for building up of Zhejiang roxburgh anoectochilus terminal bud tissue-culturing quick-propagation system, including protocorms are induced, breed, divided
Change and take root step,
Wherein, with MS as minimal medium, it is minimal medium to take root with 1/2 MS for the induction of protocorms, propagation and differentiation,
Plant growth regulator, pH 5.8 are added, fluid nutrient medium sucrose mass percent concentration is 5.0%, solid medium sucrose matter
Amount percent concentration is 3.0%, and the banana puree of mass content 10% is added in protocorms differential medium；
1）Protocorms are induced：Using fluid nutrient medium or solid medium,
The mg/L containing 0.6 mg/L+NAA of TDZ 0.4 in fluid nutrient medium；
Solid medium mg/L containing 0.6 mg/L+NAA of TDZ, 0.2 mg/L+6-BA 2.0；
2）Protocorms are bred：
The mg/L containing 1.5 mg/L+6-BA of S 3307,1.0 mg/L+NAA 0.2 in increment culture medium, wherein S 3307 is
The Main Factors of protocorms propagation；
3）Protocorms break up：Protocorms are carried out with differentiation culture, 10 days protocorms spheroid tops start projection and come to a point, become
It is green, the seedling with stipes, blade and immature hairy root can be divided into after 60 days,
The mg/L containing 0.5 mg/L+NAA of 6-BA 0.1 in differential medium；
4）Plant takes root：The seedling of differentiation to be cultivated in root media and begin with root after 10 d and grow, and contains in root media
NAA 1.0 mg/L + IBA 1.0 mg/L。
Described 1）Protocorms are induced, and take the aseptic seedling of healthy growth, and its blade is cut from shank on superclean bench
Remove, aerial root cuts off from base portion, stem is cut into containing the 2-4 stem section of section.
Described 1）Protocorms are induced, and using fluid nutrient medium, inoculation for the first time changes culture medium after 3 days, often later
7 days 1 subcultures of replacing, 90 r/min, the h/d of light application time 12,3000 lx, temperature is 25 DEG C.
The condition of culture that the protocorms solid induces, breeds, breaks up and takes root is：3000 lx, light application time 12
H/d, temperature is 25 DEG C.
The beneficial effects of the invention are as follows in order to solve the problems, such as scarcity of resources, it is necessary to set up the optimal body of its quick breeding
System.Therefore the application inquires into different plant hormones with the induction of comparison protocorms, propagation, differentiation with Zhejiang roxburgh anoectochilus terminal bud as material
And the influence taken root, the optimal system that plant quickly breeds is set up, it is that the Amplification Culture of Zhejiang roxburgh anoectochilus terminal bud lays the first stone, also it is the present
Research afterwards to aspects such as Zhejiang roxburgh anoectochilus terminal bud compositions and exploitation offer condition.Result of study is to solving roxburgh anoectochilus terminal bud base simultaneously
Contradiction between conservation of plant resources and rationally utilization has certain directive significance.
Brief description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the induced map of Zhejiang roxburgh anoectochilus terminal bud protocorms；
Fig. 2 is the vegetative map of Zhejiang roxburgh anoectochilus terminal bud protocorms；
Fig. 3 is Zhejiang roxburgh anoectochilus terminal bud protocorms differentiation figure；
Wherein part A：Differentiation culture 10d；Part B：Differentiation culture 60d；
Fig. 4 is the culture of rootage situation map of Zhejiang roxburgh anoectochilus terminal bud after 60 d,
Wherein part A：No. 4 culture mediums；Part B：No. 7 culture mediums；
Fig. 5 is the culture of rootage situation map of Zhejiang roxburgh anoectochilus terminal bud after 120 d,
Wherein part A：No. 4 culture mediums, part B：No. 7 culture mediums；
Fig. 6 is the culture of rootage situation map of Zhejiang roxburgh anoectochilus terminal bud after 180 d,
Wherein part A：No. 4 culture mediums, B parts：No. 7 culture mediums.
Specific embodiment
First, materials and methods
1.1 materials
Zhejiang roxburgh anoectochilus terminal bud（ZJJ）Tissue-cultured seedling is provided by Tongji University-Lishui Institute of Traditional Chinese Medicine's resource plant laboratory.
1.2 methods
1.2.1 the preparation of culture medium
The induction of protocorms, propagation and break up with MS as minimal medium, culture of rootage with 1/2 MS be minimal medium, plus
Enter the plant growth regulator of various concentrations, pH 5.8, fluid nutrient medium sucrose mass percent concentration is 5.0%, solid culture
Base sucrose mass percent concentration is 3.0%, 10% banana puree of extra addition in protocorms differential medium.All culture mediums
121 DEG C of min of autoclaving 20, cool down standby.
1.2.2 the induction of protocorms
Liquid Culture induces protocorms.Choosing each 0.2 mg/L of TDZ and NAA, 0.4 mg/L, 0.6 mg/L, 3 kinds of concentration is carried out
Experiment.The aseptic seedling of healthy growth is taken, its blade is cut off from shank on superclean bench, aerial root cuts off from base portion, will
Stem is cut into containing the 2-4 stem section of section, is inoculated at random in each group culture medium, every group 4 bottles, and every bottle is inoculated with 10, inoculation 3 for the first time
Culture medium is changed after d, every 7 d changes 1 subculture later, and statistics induces the stem section number of protocorms after 31 d of inoculation,
Average and calculate its inductivity.Inductivity=（Induce protocorms stem section number/inoculation stem section number）× 100% .
Solid culture induces protocorms.Choose the mg/L of TDZ 0.2,0.3 mg/L, 0.4 mg/L, 0.6 mg/L, NAA 0.2
Totally 5 kinds of concentration proportioning combinations are tested for mg/L, 0.4 mg/L and the mg/L of 6-BA 2.0.Stem section treatment is identical with 3.2.2.1,
It is inoculated into each group culture medium at random, every group 3 bottles, every bottle 7-8, every 7 d changes 1 subculture, is counted after 30 d of inoculation
The stem section number of protocorms is induced, average calculating its inductivity and induced efficiency.Inductivity=（Induce protocorms
Stem section number/inoculation stem section number）× 100% ；Induced efficiency=（The protocorms number for inducing/inoculation stem section number）× 100%
1.2.3 the propagation of protocorms
Choose the mg/L of S 3307 0.5,1.0 mg/L, 1.5 mg/L, 6-BA1.0 mg/L, 2.0 mg/L, 3.0 mg/L and NAA
Each 3 concentration of 0.2 mg/L, 0.4 mg/L, 0.6 mg/L is tested.The stem section with protocorms that will be induced connects at random
Plant in each group culture medium, every group 5 bottles, every bottle is inoculated with 10-15,60 d statisticses, calculating growth coefficient of averaging.
Growth coefficient=（Breed the protocorms number/inoculation stem section number for）× 100% .
1.2.4 the differentiation of protocorms
Choose the mg/L of 6-BA 0.5,1.0 mg/L, 2.0 mg/L and each 3 kinds of the mg/L of NAA 0.1,0.2 mg/L, 0.3 mg/L
Concentration is tested.Protocorms cutting after Multiplying culture is agglomerating, it is inoculated at random in each group culture medium, every group 5 bottles, often
5 protocorms groups of bottle inoculation, culture 60 d statistics differentiation situations, calculating differentiation rate of averaging.Differentiation rate=（The class of differentiation
Protocorm stem number/inoculation protocorms number）× 100%.
1.2.5 culture of rootage
Each 0.5 mg/L of IBA and NAA, 1.0 mg/L, 1.5 mg/L, 3 kinds of concentration are chosen to be tested.To break up what culture was obtained
The tender seedling of children（About 2.0 cm）In Stochastic accessing root media, every group 3 bottles, 7 tender seedlings of -8 childrens of every bottle of inoculation are often cultivated
60 d count the situation, calculating rooting rate of averaging of taking root.Rooting rate=（Take root the young young tender seedling number of tender seedling number/inoculation）×
100% 。
1.2.6 condition of culture
Protocorms liquid induction condition of culture be：90 r/min, light application time 12 h/d, 3000 lx, 25 DEG C；
The condition of culture that protocorms solid induces, breeds, breaks up and takes root is：3000 lx, the h/d of light application time 12, temperature
It is 25 DEG C.
2nd, result and analysis
The induction of 2.1 protocorms
2.1.1 Liquid Culture induces protocorms
After the d of Fiber differentiation 10, internode position forms 2-6 milky protocorms to stem section（Fig. 1）, united after 30 d of culture
Meter induces the stem section number of protocorms, such as table 1.Result shows：TDZ and NAA is used cooperatively and can be induced Zhejiang roxburgh anoectochilus terminal bud stem section
Protocorms are grown, but different hormones influences different to the inducing effect of protocorms.When the timing of TDZ concentration one, with NAA
The inductivity of the increase protocorms of concentration does not have significant change, and such as TDZ concentration is 0.2 mg/L, and NAA concentration is respectively 0.2
When mg/L, 0.4 mg/L, 0.6 mg/L, the inductivity of protocorms is followed successively by 10.8%, 12.5%, 9.2%.When NAA concentration one
Regularly, with the increase of TDZ concentration, the inductivity of protocorms has and more significantly increases, such as when NAA concentration is 0.2
When the concentration of mg/L, TDZ is respectively 0.2 mg/L, 0.4 mg/L, 0.6 mg/L, the inductivity of protocorms is followed successively by 10.8%,
29.2%、50.0%.It can be seen that influences of the TDZ to the inducing effect of protocorms is more than NAA.In all hormone combinations, No. 8 cultures
The inductivity highest of base class protocorm, reaches 62.5%.Accordingly, it is preferred that inductive condition is respectively 0.6 for the concentration of TDZ and NAA
Mg/L and 0.4 mg/L.
2.1.2 solid culture induces protocorms
Similar with Liquid Culture induction protocorms, solid Fiber differentiation one week or so starts breast occur at stem section internode position
White protocorms.The stem section number for inducing protocorms is counted after cultivating 30 d, as a result as shown in table 2.TDZ, NAA and 6-BA
By different proportionings using can induce Zhejiang roxburgh anoectochilus terminal bud stem section to grow protocorms, but hormon is former with roxburgh anoectochilus terminal bud class is compared
The inducing effect of bulb is different, the inducibility of No. 3, No. 4, No. 5 culture mediums is preferable from from the point of view of inductivity, and protocorms are lured
Conductance does not exist obvious difference between 3, wherein No. 3 inductivity highests of culture medium, reach 68.0%.Come from induced efficiency
See, No. 5 induced efficiency highests of culture medium, are 144.6%, are significantly higher than other 4 groups.Therefore comprehensive inductivity and induced efficiency,
Protocorms induction optimum condition is the mg/ of 0.6 mg/L+NAA of TDZ, 0.2 mg/L+6-BA 2.0 in solid medium
L。
The propagation of 2.2 protocorms
The stem section that protocorms will be induced is transferred on solid multiplication culture medium, observes that the class of new propagation is former after 60 d of culture
Bulb is large number of, growth is vigorous（See Fig. 2）.Statistics protocorms number, calculates growth coefficient, the results are shown in Table 3.As a result table
It is bright：S 3307,6-BA and NAA are used cooperatively breeds effectively Zhejiang roxburgh anoectochilus terminal bud protocorms, and different hormone combinations pair
The cultivation effect of protocorms has differences, wherein No. 8 growth coefficient highest of culture medium, is 8.4.From the analysis of extreme difference R values
Understand, S 3307 plays a major role to the propagation of protocorms, be secondly 6-BA, influence minimum is NAA.According to k value sizes,
The optimum condition that protocorms propagation can be drawn is the mg/ of 1.5 mg/L+6-BA of S 3307,2.0 mg/L+NAA 0.2
L。
2.2.1 the differentiation of protocorms
Protocorms are carried out with differentiation culture, 10 d or so protocorms spheroid top beginning projection to come to a point, greening, then gradually
Elongation, differentiates tender shoots（Fig. 3 A）, the seedling with stipes, blade and immature hairy root can be divided into after 60 d（Fig. 3 B）.
Protocorms number to breaking up is counted, and calculates differentiation rate.As shown in table 4, different hormone combinations are to class protocorm for result
The differentiation effect of stem produces Different Effects.When the timing of 6-BA concentration one, with the increase of NAA concentration, differentiation rate is gradually reduced, example
Such as, when 6-BA concentration is 1.0 mg/L, and NAA concentration is 0.1 mg/L, 0.2 mg/L, 0.3 mg/L, differentiation rate is respectively
73.3%、65.3%、57.3%.When the timing of NAA concentration one, with the increase of 6-BA concentration, differentiation rate is gradually reduced, for example, working as
NAA concentration is 0.2mg/L, when 6-BA concentration is 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, differentiation rate is respectively 81.3%,
65.3%、46.7%.Therefore No. 1 differentiation rate highest of culture medium, the optimum condition of differentiation is the mg/L+NAA 0.1 of 6-BA 0.5
mg/L。
2.2.2 culture of rootage
The seedling of differentiation to be cultivated in root media and begin with root after 10 d and grow, after the d of culture of rootage 60 statistics take root number and
Plant growth condition（Fig. 4）, rooting rate is calculated, as a result as shown in table 5.7 kinds of culture mediums can induce Zhejiang roxburgh anoectochilus terminal bud to take root, and
Hormon matches that influence to rooting efficiency is different, and the rooting rate of wherein 1-6 culture mediums has all reached 100%, but from life
The integral status of the plant strain growth such as radical, root be long see that preferably, mean elements is 1.51 to No. 4 upgrowth situations of culture medium, and root is long
It is 1.53 cm, the cm of plant height 7.90, stipes number 6.50, the upgrowth situation of No. 7 culture mediums is worst, and rooting rate is only 70%, strain
The index such as height, stipes number, mean elements is below other 6 groups of culture mediums.Therefore the optimum condition of Zhejiang roxburgh anoectochilus terminal bud culture of rootage
It is the mg/L of 1/2 MS+NAA, 1.0 mg/L+IBA 1.0.
After 60 d, that is, add up 120 d of culture and observe and count plant condition of rooting again（Fig. 5）, as a result as shown in table 6, with
The extension of incubation time, root is on the increase elongated, and plant constantly grows.See on the whole in No. 4 culture mediums and No. 6 culture mediums and plant
Strain upgrowth situation is all preferable, average plant height, stipes number, root be long and radical between 2 without significant difference.No. 7 culture mediums it is flat
Equal plant height, stipes number, root long, radical and rooting rate also all increased, and rooting rate reaches 92%, but compared to other several groups of entirety
Upgrowth situation is still worst.
I.e. 180 d of accumulative culture, observe the condition of rooting of plant again after 60 d（Fig. 6）, as a result as shown in table 7.Plant entirety
All constantly growing, the gap of upgrowth situation is gradually reduced between 7 groups of plant, wherein the rooting rate of the plant in No. 7 culture mediums reaches
To 100%, it is seen that with the extension of incubation time, rooting rate is continuously increased, but its upgrowth situation with other group than still compared with
Difference.Upgrowth situation is preferably No. 4 culture mediums and No. 5 culture mediums.
Comprehensive 3 experimental results find, with the extension of incubation time, number of taking root is on the increase, and root is constantly elongated, averagely
Plant height constantly increases, and inductivity is constantly raised, and roxburgh anoectochilus terminal bud plant growth condition in Zhejiang is best always on No. 4 culture mediums, No. 7 trainings
The upgrowth situation supported on base is then worst all the time.It can be seen that preferred culture of rootage condition is 1/2 MS+NAA1.0 mg/L+IBA
1.0 mg/L, rooting rate is up to 100%.
Found during Zhejiang roxburgh anoectochilus terminal bud rapid propagation system is set up, the plant hormone of various concentrations is former with class is compared
The induction of bulb, generation large effect of breeding, break up and take root.
For the induction of protocorms, in different culture mediums, preferably hormone combination is different.In Liquid Culture and solid
In culture preferred protocorms inductive condition be respectively the mg/L of 0.6 mg/L+NAA of the TDZ 0.4 and mg/L of TDZ 0.6+
The mg/L of 0.2 mg/L+6-BA of NAA 2.0, inductivity is respectively 62.5%, 59.3%.What protocorms were bred, and broke up, taking root
It is preferred that hormone combinations are followed successively by：1.5 mg/L+6-BA of S 3307,1.0 mg/L+NAA 0.2,0.5 mg/ of mg/L, 6-BA
The mg/L of L+NAA 0.1, the mg/L of 1/2 MS+NAA, 1.0 mg/L+IBA 1.0, growth coefficient, differentiation rate, rooting rate
Respectively 8.4,87.3%, 100%, wherein S 3307 is the Main Factors for promoting protocorms propagation.
Claims (4)
1. a kind of method for building up of Zhejiang roxburgh anoectochilus terminal bud tissue-culturing quick-propagation system, it is characterized in that：Induced including protocorms,
Breed, break up and take root step,
Wherein, with MS as minimal medium, it is minimal medium to take root with 1/2 MS for the induction of protocorms, propagation and differentiation,
Plant growth regulator, pH 5.8 are added, fluid nutrient medium sucrose mass percent concentration is 5.0%, solid medium sucrose matter
Amount percent concentration is 3.0%, and the banana puree of mass content 10% is added in protocorms differential medium；
1）Protocorms are induced：Using fluid nutrient medium or solid medium,
The mg/L containing 0.6 mg/L+NAA of TDZ 0.4 in fluid nutrient medium；
Solid medium mg/L containing 0.6 mg/L+NAA of TDZ, 0.2 mg/L+6-BA 2.0；
2）Protocorms are bred：
The mg/L containing 1.5 mg/L+6-BA of S 3307,1.0 mg/L+NAA 0.2 in increment culture medium, wherein S 3307 is
The Main Factors of protocorms propagation；
3）Protocorms break up：Protocorms are carried out with differentiation culture, 10 days protocorms spheroid tops start projection and come to a point, become
It is green, the seedling with stipes, blade and immature hairy root can be divided into after 60 days,
The mg/L containing 0.5 mg/L+NAA of 6-BA 0.1 in differential medium；
4）Plant takes root：The seedling of differentiation to be cultivated in root media and begin with root after 10 d and grow, and contains in root media
NAA 1.0 mg/L + IBA 1.0 mg/L。
2. method for building up according to claim 1, it is characterized in that：Described 1）Protocorms are induced, and take the nothing of healthy growth
Vaccine, cuts off its blade from shank on superclean bench, and aerial root cuts off from base portion, and stem is cut into containing the 2-4 stem of section
Section.
3. method for building up according to claim 1, it is characterized in that：Described 1）Protocorms are induced, using fluid nutrient medium,
Culture medium is changed in inoculation for the first time after 3 days, change 1 subculture, 90 r/min, the h/ of light application time 12 within every 7 days later
D, 3000 lx, temperature are 25 DEG C.
4. method for building up according to claim 1, it is characterized in that：The protocorms solid induction, propagation, differentiation and life
The condition of culture of root is：3000 lx, the h/d of light application time 12, temperature are 25 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113080058A (en) * | 2021-03-24 | 2021-07-09 | 福建省中科生物股份有限公司 | Method for promoting proliferation and differentiation of Anoectochilus roxburghii corms |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461118A (en) * | 2013-07-31 | 2013-12-25 | 湖州市农业科学研究院 | Industrialized production method for anoectochilus roxburghii seedlings |
| CN103858762A (en) * | 2014-03-17 | 2014-06-18 | 厦门涌泉科技有限公司 | Rapid proliferation method for anoectochilus formosanus tissue culture |
| CN104823846A (en) * | 2015-04-24 | 2015-08-12 | 福建省农业科学院农业生物资源研究所 | Rapid breeding method of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings |
-
2017
- 2017-01-23 CN CN201710048795.7A patent/CN106888965A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461118A (en) * | 2013-07-31 | 2013-12-25 | 湖州市农业科学研究院 | Industrialized production method for anoectochilus roxburghii seedlings |
| CN103858762A (en) * | 2014-03-17 | 2014-06-18 | 厦门涌泉科技有限公司 | Rapid proliferation method for anoectochilus formosanus tissue culture |
| CN104823846A (en) * | 2015-04-24 | 2015-08-12 | 福建省农业科学院农业生物资源研究所 | Rapid breeding method of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 杨柏云等: "金线莲原球茎的诱导与快速繁殖", 《安徽农业科学》 * |
| 王建明等: "金线莲组织培养的条件优化研究", 《中国现代中药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113080058A (en) * | 2021-03-24 | 2021-07-09 | 福建省中科生物股份有限公司 | Method for promoting proliferation and differentiation of Anoectochilus roxburghii corms |
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Inventor after: Chen Changli Inventor after: Zhu Guanlin Inventor after: Jin Guanrong Inventor after: Luo Xiahong Inventor after: Tang Nannan Inventor after: Yu Yang Inventor after: Li Shan Inventor after: Cheng Zhou Inventor after: An Xia Inventor after: Li Wenlue Inventor before: Chen Changli Inventor before: Jin Guanrong Inventor before: Luo Xiahong Inventor before: Tang Nannan Inventor before: Yu Yang Inventor before: Li Shan Inventor before: Cheng Zhou Inventor before: Zhu Guanlin |
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Application publication date: 20170627 |