CN106885780A - A kind of method for determining soil nitrate-N - Google Patents
A kind of method for determining soil nitrate-N Download PDFInfo
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- CN106885780A CN106885780A CN201510944782.9A CN201510944782A CN106885780A CN 106885780 A CN106885780 A CN 106885780A CN 201510944782 A CN201510944782 A CN 201510944782A CN 106885780 A CN106885780 A CN 106885780A
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- 238000000034 method Methods 0.000 title claims abstract description 48
- 239000002689 soil Substances 0.000 title claims abstract description 44
- 238000002835 absorbance Methods 0.000 claims abstract description 34
- 238000004821 distillation Methods 0.000 claims abstract description 27
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 20
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000002386 leaching Methods 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 14
- 238000000205 computational method Methods 0.000 claims abstract description 12
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 6
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 44
- 238000002798 spectrophotometry method Methods 0.000 claims description 17
- 238000005259 measurement Methods 0.000 claims description 9
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 238000012937 correction Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 239000004323 potassium nitrate Substances 0.000 claims description 3
- 235000010333 potassium nitrate Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 16
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 8
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 30
- 239000000243 solution Substances 0.000 description 27
- 239000001103 potassium chloride Substances 0.000 description 15
- 235000011164 potassium chloride Nutrition 0.000 description 15
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 229910052793 cadmium Inorganic materials 0.000 description 3
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
- 229910001199 N alloy Inorganic materials 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000618 nitrogen fertilizer Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- MKNQNPYGAQGARI-UHFFFAOYSA-N 4-(bromomethyl)phenol Chemical compound OC1=CC=C(CBr)C=C1 MKNQNPYGAQGARI-UHFFFAOYSA-N 0.000 description 1
- VRZJGENLTNRAIG-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]iminonaphthalen-1-one Chemical compound C1=CC(N(C)C)=CC=C1N=C1C2=CC=CC=C2C(=O)C=C1 VRZJGENLTNRAIG-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000021384 green leafy vegetables Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
It is specifically a kind of method for determining soil nitrate-N and nitrite nitrogen summation the present invention relates to a kind of method for determining soil nitrate-N.Pedotheque is vibrated into extraction with 4 times of 2mol/L KCl solution of bulking value (g/ml), filtering, gained leaching liquor uses A in 220nm and 275nm absorbances are determined on ultraviolet-visible spectrophotometer220–2×A275Computational methods try to achieve absorbance, and establishing criteria curve, nitrate in inquiry leaching liquor.The concentration passes through formula：Y=1.021x-0.223 is corrected, and as a result determining ammonium nitrogen with the way of distillation is combined, and is set up complete set, efficient, the accurate way of distillation and is determined the inorganic nitrogen systems of soil available.The inventive method is reproducible, and reappearance is high, simple and easy to apply.
Description
Technical field
The present invention relates to the measure of nitrified nitrogen in soil, specifically a kind of measure side for improving soil nitrate-N
Method.
Background technology
Inorganic nitrogen can be divided into water-soluble state, exchange state and stationary state etc. in soil.Determine ammonium state, the nitre state in soil
And nitrite nitrogen element, typically determine both water-soluble state and exchange state total amount.Exchange state is can be by neutral salt solution
Exchange the part extracted (neutral salt solution is potassium chloride or sodium chloride).Neutral salt solution mixes with soil, shakes
Swing, the ammonium nitrogen of adsorption by soil is exchanged and is leached, wherein also including water-soluble state.This extract is through filtering, filtrate
The measure of various form nitrogen is carried out again.Conventional measure ammonium nitrogen method has the way of distillation and indophenol blue colorimetry, so
And the method for determining nitrate nitrogen has a lot, including the way of distillation, disulfonic acid phenol, cadmium reducing process and dual wavelength are purple
Outer AAS (document 1：Lu Rukun, soil agrochemistry analysis method, Beijing：Chinese agriculture science and technology
Publishing house, 2000：156-165).
It should be noted that nitrite nitrogen falls within exchange state nitrogen, in process of crop growth, as effective nitrogenous fertilizer
For crop provides nutrient, even if its content and low, the 5% of not enough nitrate nitrogen.Said determination soil nitrate-N
The value determined in method can contain nitrite nitrogen.Herein involved by us be determine soil nitrate-N and Asia
The method of nitrate nitrogen summation.
Conventional measure nitrate nitrogen method has the disadvantage that：The way of distillation needs specific device, and energy resource consumption is big, and
Determine nitrogen alloy and be difficult the mesh sieve of levigate mistake 100；Disulfonic acid phenol operating procedure is excessively cumbersome, soil extraction moisture
Evaporation time is long, take the energy and many kinds of substance has interference；Although Cadmium reduction column is disturbed less, high precision,
Cumbersome and cadmium is highly toxic heavy metal, easily pollutes environment；Ripple is determined in dual-wavelength ultraviolet spectrophotometry
No unified standard (document 2 long：Evergreen, Wen Xinrong is applied, dual-wavelength ultraviolet spectrophotometry determines soil nitre
State nitrogen, soil and fertilizer, 2006 (1)：50-51).
The content of the invention
Simple to operate it is an object of the invention to provide one kind, reproducible without any reagent, reappearance is high
Measure soil nitrate-N method.
To achieve the above object, the technical solution adopted by the present invention is as follows：
A kind of method for determining soil nitrate-N,
1) with 2mol/L KCl as solvent compound concentration be more than 6 in the range of 0.1-20mg N/L not
With the nitrate nitrogen solution of concentration, respectively with ultraviolet-visible spectrophotometer at 220nm and 275nm wavelength
Determine each and prepare solution absorbance A220And A275；Use A220–2×A275Computational methods try to achieve absorbance,
With nitrate as abscissa, the absorbance of calculating is ordinate making standard curve；
2) prepared by soil extraction：By pedotheque with 4 times of 2mol/L KCl solution of bulking value (g/ml)
Vibration extraction more than 1h, filtering obtains leaching liquor；
3) leaching liquor is placed directly within ultraviolet-visible spectrophotometer and determines 220nm and 270nm absorbances
Value, uses A220–2×A275Computational methods try to achieve absorbance, by the absorbance tried to achieve in step 1) make
Standard curve in inquire about obtain leaching liquor in nitrate；
4) correction of ultraviolet spectrophotometry method measured value is turned into way of distillation measured value, formula is
Y=1.021x-0.223, wherein x are the soil nitrate-N concentration measurement of ultraviolet spectrophotometry, and y is distillation
The soil nitrate-N concentration measurement of method.
The step 1) prepare nitrate nitrogen solution detailed process be：It is solvent compound concentration with 2mol/L KCl
It is the nitrate nitrogen solution of more than 6 various concentrations in the range of 0.1-20mg N/L, including 0.1mg
The nitrate nitrogen solution of bis- end value concentration of N/L and 20mg N/L, and more than 0.1mg N/L to less than 20
The nitrate nitrogen solution of more than 4 various concentrations of mg N/L.
The step 1) prepare nitrate nitrogen solution detailed process be：It is solvent compound concentration with 2mol/L KCl
It is the nitrate nitrogen solution of more than 6 various concentrations in the range of 0.1-15mg N/L, including 0.1mg
The nitrate nitrogen solution of bis- end value concentration of N/L and 15mg N/L, and more than 0.1mg N/L to being less than
The nitrate nitrogen solution of more than 4 various concentrations of 15mg N/L.
The step 1) detailed process be：Respectively draw concentration for 100mg N/L nitrate nitrogen solution 0.05,
0.25th, 0.5,2.5,5.0,7.5mL adds 2mol/L KCl to 50mL to carve in 50mL volumetric flasks
Degree line, shake up, with ultraviolet-visible spectrophotometer at 220nm and 275nm wavelength mensuration absorbance；
Use A220–2×A275Computational methods try to achieve absorbance, with nitrate as abscissa, the absorbance of calculating
It is worth for ordinate makes standard curve, the standard curve range of linearity is 0.1-15mg N/L, standard curve one
As be polynomial equation.
The solute that the preparation nitrate nitrogen solution is used is potassium nitrate.
Detailed process can be：A kind of method for determining soil nitrate-N,
1) standard curve is made：100mg N/L nitrate nitrogens solution (2mol/L KCl preparations) 0.05,0.25 are drawn,
0.5,2.5,5.0,7.5mL in 50mL volumetric flasks, add 2mol/L KCl constant volumes, shake up,
With ultraviolet-visible spectrophotometer in 220nm and 275nm wavelength mensuration absorbances, A is used220–2×A275
Computational methods try to achieve absorbance, with nitrate as abscissa, the absorbance of calculating is ordinate
Make standard curve；
2) prepared by soil extraction：Pedotheque is vibrated with 4 times of 2mol/L KCl solution of bulking value (g/ml)
Extraction 1h, filtering obtains leaching liquor；
3) leaching liquor is placed directly within ultraviolet-visible spectrophotometer and determines 220nm and 270nm absorbances,
Use A220–2×A275Computational methods try to achieve absorbance, query criteria curve calculates nitrate nitrogen in leaching liquor
Concentration；
4) correction of ultraviolet spectrophotometry method measured value is turned into way of distillation measured value, formula is y=1.021x-0.223
(R2=0.9722, n=20), wherein x is ultraviolet spectrophotometry method measured value, and y is surveyed for the way of distillation
Definite value.
Content of Nitrate-Nitrogen in Soil is determined using the method, is had the following advantages：
1. the method is easy to operate, and step is few, without adding any reagent, directly carries out the UV absorption of leaching liquor
Determine, human error is small.
2. the method saves trouble.Original way of distillation or colorimetric method determine nitrate nitrogen for one day no more than 100, identical
1000 can be determined with the method in time, efficiency improves 9 times.
3. the soil nitrate nitrogen concentration and the fabulous (R of way of distillation measured value correlation for being determined using the method2=0.9722,
N=20), therefore after can measured value be corrected determine ammonium nitrogen with the way of distillation to be combined, set up a set of complete
Whole, efficient, the accurate inorganic nitrogen determination method system of soil available.
The inventive method is reproducible, and reappearance is high, simple and easy to apply.
Brief description of the drawings
Fig. 1 nitrate nitrogen bioassay standards curve -1；
Fig. 2 nitrate nitrogen bioassay standards curve -2；
Fig. 3 way of distillations and Soil by Spectrophotometry nitrate nitrogen result are compared；
Fig. 4 verifies the correlation of the way of distillation and determined by ultraviolet spectrophotometry nitrate nitrogen.
Specific embodiment
Embodiment 1
Set up determined by ultraviolet spectrophotometry nitrate nitrogen standard curve
Because nitrate is directly determined without any operation in soil extraction, therefore nitrate should
Fully fall in the standard curve range of linearity, otherwise measurement result is inaccurate.It is computed, when nitre in the soil liquid
When state nitrogen concentration is 15mg N/L, content of soil nitrate-N is about 66kg/mg, equivalent to amount of application of nitrogen fertilizer
(the 0-20cm soil weight is 1.2g/cm to 158kg N/ha3), therefore nitrate nitrogen Cmax sets in standard curve
15m N g/L (if desired, it can also be provided that 20mg N/L) are set to, experiment need can be met completely
Will.
After measured, there is stronger suction all at 220nm in determined by ultraviolet spectrophotometry nitrate nitrogen and nitrite nitrogen
Receive peak, therefore the measurement result of the method contains nitrite nitrogen content, this nitrate nitrogen determined with the way of distillation and
Nitrite nitrogen summation matches, as the experiment basis that the method and the way of distillation are mutually authenticated.In addition, soil is sub-
Nitrate nitrogen is the pilot process of soil nitrification, and content is extremely low, is generally ignored in continuous mode, because
This calibration curve solution is added without standard nitrite nitrogen, and its absorbance control nitrate nitrogen is calculated.
Detailed process is as follows：
1) nitrate nitrogen storing solution is prepared：It is accurate to weigh 7.216g drying potassium nitrate, add 2mol/L KCl molten
Solution, is settled in 1L volumetric flasks, and this solution contains nitrate nitrogen 1000mg/L.This storing solution is pressed into 10 multiples
Dilution, 2mol/L KCl constant volumes obtain the solution that concentration is 100mg N/L；
2) standard curve is made：Draw above-mentioned nitrate nitrogen solution 0.05,0.25,0.5,2.5,5.0,7.5mL
In 50mL volumetric flasks, add 2mol/L KCl to graduation mark, shake up, use ultraviolet-visible spectrophotometer
The mensuration absorbance at 220nm and 275nm wavelength.Use A220–2×A275Computational methods try to achieve absorbance,
With nitrate as abscissa, the absorbance of calculating is ordinate making standard curve.Result such as Fig. 1
Shown, standard curve meets polynomial equation, R2=0.998 (n=7).
Embodiment 2
Ultraviolet spectrophotometry determines nitrate and compares with the way of distillation
Specific implementation process：
1) prepared by soil extraction：By pedotheque (common n=44 pedotheque) respectively with 4 times
The 2mol/L KCl solution vibration extraction 1h of bulking value (g/ml), filtering obtains leaching liquor.This extraction sample
It is divided into two parts, a part of (common n=20 pedotheque) is used for determining that two methods determine nitrate
Correlation, remaining sample (common n=24 pedotheque) is used to verify the accuracy of the correlation；
2) standard curve is made：It is the nitrate nitrogen solution 0.05,0.25 of 100mg N/L to draw concentration,
0.5,2.5,5.0,7.5mL in 50mL volumetric flasks, add 2mol/L KCl to graduation mark, shake up,
With ultraviolet-visible spectrophotometer at 220nm and 275nm wavelength mensuration absorbance.Use A220–2×A275
Computational methods try to achieve absorbance, with nitrate as abscissa, the absorbance of calculating is that ordinate makes
Standard curve (Fig. 2)；Standard curve is generally polynomial equation；
3) nitrate in determined by ultraviolet spectrophotometry leaching liquor：By step 1) in leaching liquor (altogether
N=20 pedotheque) measure 220nm and 270nm in ultraviolet-visible spectrophotometer is placed directly within respectively
Absorbance A220And A275, use A220–2×A275Computational methods try to achieve absorbance, inquiry above-mentioned standard is bent
Nitrate in line computation leaching liquor；
4) way of distillation determines nitrate in leaching liquor：Said determination step 3 is taken respectively) same leaching
Extract (common n=20 pedotheque) 50mL in cucurbit, while it is (interior to do blank group in a cucurbit
Equipped with 50mL 2mol/L KCl solution) compareed, 1g magnesia is separately added into, it is placed in distilling apparatus
In, heating distillation is carried out to being passed through water vapour in leaching liquor and blank group respectively immediately, received to condenser pipe lower end
When distillate volume reaches 100mL in the triangular flask of distillate, terminate distillation, remove the triangle for receiving distillate
Bottle, this step purpose is removal ammonium nitrogen.Change the triangular flask for receiving distillate into one and fill 15mL weight
Concentration be 2% boric acid plus one drop (0.05mL) mixed indicator (0.066g methyl reds and 0.099g bromocresol greens
Be settled to 100ml with the ethanol of volumetric concentration 95%) receiving bottle in condenser pipe lower end, continue respectively to cucurbit
Middle addition 0.2g determines nitrogen alloy, and (alloy is constituted：Cu 50%, Al 45%, Zn 5%), continue respectively to distillation
Water vapour is passed through in bottle to be distilled, when the distillate volume into receiving bottle reaches 100mL, terminate distillation,
Remove receiving bottle.Use 0.003molL-1Sulfuric acid standard liquid is titrated, and red is manifested in solution is by grey
When be titration end-point.Sulfuric acid amount according to sample consumption calculates nitrate nitrogen content (document 1：Lu Rukun, soil
Earth agrochemistry analysis method, Beijing：Chinese agriculture Science Press, 2000：156-165)；
Measurement result is shown in Fig. 3, and the variation of the soil extraction nitrate that randomly selects is larger, scope from
0.1-3.7mg/L is representative strong.Coefficient R=0.986 of two methods, illustrates the correlation of two methods
Property preferably, ultraviolet spectrophotometry can be used to replace the cumbersome way of distillation, for determining content of soil nitrate-N.
5) remaining 24 soil extractions are taken, according to above-mentioned 3) -4) described in step, use two kinds of sides
Method is measured to its nitrate, and by determined by ultraviolet spectrophotometry result according to formula y=1.021x-
0.223 correction, acquired results are compared with way of distillation measurement result, as a result as shown in Figure 4.Result shows
Two methods correlation is fabulous, particularly the very low sample of concentration, and measurement result is still very identical, illustrates operation
Simple ultraviolet spectrophotometry can replace the cumbersome way of distillation completely, as measure soil nitrate-N
Important method.
Claims (5)
1. it is a kind of determine soil nitrate-N method, it is characterised in that：
1) with 2mol/L KCl as solvent compound concentration be more than 6 in the range of 0.1-20mg N/L not
With the nitrate nitrogen solution of concentration, respectively with ultraviolet-visible spectrophotometer at 220nm and 275nm wavelength
Determine each and prepare solution absorbance A220And A275；Use A220–2×A275Computational methods try to achieve absorbance,
With nitrate as abscissa, the absorbance of calculating is ordinate making standard curve；
2) prepared by soil extraction：By pedotheque with 4 times of 2mol/L KCl solution of bulking value (g/ml)
Vibration extraction more than 1h, filtering obtains leaching liquor；
3) leaching liquor is placed directly within ultraviolet-visible spectrophotometer and determines 220nm and 270nm absorbances
Value, uses A220–2×A275Computational methods try to achieve absorbance, by the absorbance tried to achieve in step 1) make
Standard curve in inquire about obtain leaching liquor in nitrate；
4) correction of ultraviolet spectrophotometry method measured value is turned into way of distillation measured value, formula is
Y=1.021x-0.223, wherein x are the soil nitrate-N concentration measurement of ultraviolet spectrophotometry, and y is distillation
The soil nitrate-N concentration measurement of method.
2. in accordance with the method for claim 1, it is characterised in that：
The step 1) prepare nitrate nitrogen solution detailed process be：It is solvent compound concentration with 2mol/L KCl
It is the nitrate nitrogen solution of more than 6 various concentrations in the range of 0.1-20mg N/L, including 0.1mg
The nitrate nitrogen solution of bis- end value concentration of N/L and 20mg N/L, and more than 0.1mg N/L to less than 20
The nitrate nitrogen solution of more than 4 various concentrations of mg N/L.
3. in accordance with the method for claim 1, it is characterised in that：
The step 1) prepare nitrate nitrogen solution detailed process be：It is solvent compound concentration with 2mol/L KCl
It is the nitrate nitrogen solution of more than 6 various concentrations in the range of 0.1-15mg N/L, including 0.1mg
The nitrate nitrogen solution of bis- end value concentration of N/L and 15mg N/L, and more than 0.1mg N/L to being less than
The nitrate nitrogen solution of more than 4 various concentrations of 15mg N/L.
4. according to the method described in claim 1 or 3, it is characterised in that：
The step 1) detailed process be：Respectively draw concentration for 100mg N/L nitrate nitrogen solution 0.05,
0.25th, 0.5,2.5,5.0,7.5mL adds 2mol/L KCl to 50mL to carve in 50mL volumetric flasks
Degree line, shake up, with ultraviolet-visible spectrophotometer at 220nm and 275nm wavelength mensuration absorbance；
Use A220–2×A275Computational methods try to achieve absorbance, with nitrate as abscissa, the absorbance of calculating
It is worth for ordinate makes standard curve, the standard curve range of linearity is 0.1-15mg N/L, standard curve one
As be polynomial equation.
5. according to any described methods of claim 1-4, it is characterised in that：The preparation nitrate nitrogen is molten
The solute that liquid is used is potassium nitrate.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107505274A (en) * | 2017-09-01 | 2017-12-22 | 湖北大学 | A kind of method system of visible spectrophotometry measure content of soil nitrate-N |
| CN109085322A (en) * | 2018-08-09 | 2018-12-25 | 中国科学院植物研究所 | A method of measurement soil microbial biomass nitrogen |
| CN112082965A (en) * | 2020-09-18 | 2020-12-15 | 武汉工程大学 | Method for determining occurrence state of residual ammonium salt in weathered crust elution-deposited rare earth ore |
| CN113252843A (en) * | 2021-04-29 | 2021-08-13 | 扬州大学 | Method for measuring available nitrogen of facility soil |
| CN114112614A (en) * | 2021-12-01 | 2022-03-01 | 浙江大学 | Method for determining ammonia nitrogen in soil by using water quality ammonia nitrogen rapid detection kit |
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| CN101545866A (en) * | 2009-05-06 | 2009-09-30 | 北京市农林科学院 | Method for measuring content of inorganic nitrogen in soil |
| CN104198419A (en) * | 2014-08-14 | 2014-12-10 | 河南农业大学 | Soil nitrate nitrogen extracting agent and method for rapid determination of soil nitrate nitrogen |
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| CN109085322A (en) * | 2018-08-09 | 2018-12-25 | 中国科学院植物研究所 | A method of measurement soil microbial biomass nitrogen |
| CN112082965A (en) * | 2020-09-18 | 2020-12-15 | 武汉工程大学 | Method for determining occurrence state of residual ammonium salt in weathered crust elution-deposited rare earth ore |
| CN113252843A (en) * | 2021-04-29 | 2021-08-13 | 扬州大学 | Method for measuring available nitrogen of facility soil |
| CN114112614A (en) * | 2021-12-01 | 2022-03-01 | 浙江大学 | Method for determining ammonia nitrogen in soil by using water quality ammonia nitrogen rapid detection kit |
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