CN106880874A - A kind of manually compound hernia paster and preparation method thereof - Google Patents
A kind of manually compound hernia paster and preparation method thereof Download PDFInfo
- Publication number
- CN106880874A CN106880874A CN201710032246.0A CN201710032246A CN106880874A CN 106880874 A CN106880874 A CN 106880874A CN 201710032246 A CN201710032246 A CN 201710032246A CN 106880874 A CN106880874 A CN 106880874A
- Authority
- CN
- China
- Prior art keywords
- preparation
- spinning
- hernia paster
- solution
- collagen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Textile Engineering (AREA)
- Materials For Medical Uses (AREA)
- Prostheses (AREA)
Abstract
The present invention relates to a kind of manually compound hernia paster and preparation method thereof, the hernia paster is the nano-fiber material of PLGA and collagen composite.Dissolved by fixing solvent containing PLGA and collagen, be prepared from using the method for conjugation electrostatic spinning.Fiber obtained by the present invention has good pattern, good mechanical property, and good degradability, well can be fitted with defective tissue position, ensure certain mechanical property in organization activity, and can be degraded in human body, the present invention can be used for the treatment of soft tissue hernia, effect is significant.
Description
Technical field
The invention belongs to hernia paster material and its preparation field, more particularly to a kind of manually compound hernia paster and its system
Preparation Method.
Background technology
Abdominal external hernia refers to abdominal visceral browser prominent to body surface by the defect of iliac region, is commonly called as " hernia ".Abdominal external hernia is
Common disease, frequently-occurring disease, its incidence of disease increase with the increase at age.There is scholar to speculate, the Chinese annual number of the infected of abdominal external hernia can
2,000,000 are can exceed that, and the unique method for curing abdominal external hernia is operation.Operation method has developed from past tension force repairing operation
The Tension-free Hernioplasty under Tension-free Hernioplasty and laparoscope till now.Traditional herniorrhaphy by force will tissue
It is stitched together, the tension force of suture is higher, has certain pain and recurrence rate is higher, postoperative mobility is poor, lives
Quality is substantially reduced.Tension-free Hernioplasty is a kind of hernia repair surgery that the nineties in 20th century proposes, is existed now
It is clinically commonly used.Because Tension-free Hernioplasty effect is substantially better than traditional operation, clinically substantially take
For traditional surgical approaches.Tension-free Hernioplasty clinically commonly used also makes the hernia repair material become research
Focus, so as to various hernia patching materials occur.Meanwhile, POI is also an insoluble problem, tradition
Material degradation it is poor, need second operation to take out mostly, bring pain to patient.The design of hernia paster material not only will
Bionical human body cell epimatrix structure and function solve the biocompatibility issues of material and cell in structure, but also should be
Ensure that the tension force of repairing cover reaches the ability of normal human's soft tissue on mechanics.Therefore repaiied from structurally and functionally structure hernia
Mending material has wide DEVELOPMENT PROSPECT.
PLGA, it is wide because it has good biodegradability and good mechanical properties
It is general for field of tissue engineering technology.But it belongs to synthesis macromolecular material, it is impossible to the biological letter for needed for nerve fiber growth is provided
Number, while hydrophily is poor, it is unfavorable for the adhesion and growth of cell.However, electrostatic spinning technique can combine the two advantage, can
Make timbering material that there is mechanical property higher and meeting human body soft tissue, and bio signal can be provided for tissue growth, promote
The adhesion of cell, increment and break up, so as to meet human body soft tissue the need for.
Collagen is a kind of natural activity albumen, with relatively low immunogenicity, good biocompatibility, itself
And its catabolite is nontoxic to cell and body, or less inflammation and immunological rejection will not be caused.Static Spinning is that one kind exists
Polymer is prepared into the technology of nanofiber, due to its multifunctionality and high efficiency, Static Spinning in the presence of high voltage electric field
It is widely used to prepare various functions nanofiber.Electro spinning nanometer fiber membrane has many significantly characteristics, such as larger ratio
Surface area, the aperture of nano-scale, flexible physical/chemical is modified etc..Because its distinctive property and function have been applied to doctor
The fields such as medicine, biotechnology, filtering, membrane technology, function textile, sensor, the energy.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of manually compound hernia paster and preparation method thereof, the present invention
It is intended to by preparing a kind of Static Spinning degradable polyester hernia repair material, final target can be to provide one preferably
Structure goes to substitute natural cytoplasmic matrix.The larger specific surface area and microcellular structure having by nano fibrous membrane, can lure
Lead fibrocyte and adhere to skin corium, and produce some important extracellular matrix components, such as collagen and cell factor,
So as to repair damaged tissues.And it is applied to the treatment of hernia.
A kind of manually compound hernia paster of the invention, the hernia paster is PLGA and collagen
Compound nano-fiber material.
The collagen is collagen.
A kind of preparation method of artificial compound hernia paster as claimed in claim 1 of the invention, including：
(1) PLGA and collagen are dissolved in solvent, configuration obtains mixed solution；
(2) by pure collagen solution solvent, pure collagen solution is obtained；
(3) above-mentioned mixed solution, pure collagen solution are carried out into conjugation electrostatic spinning as spinning solution, is obtained final product.
The mass ratio of PLGA and collagen is (58-62) in the step (1)：(38-42).
It is preferred that the mass ratio of PLGA and collagen is 60：40.
Solvent is hexafluoroisopropanol in the step (1), (2) (purity is 99%).
The mass percentage concentration of mixed solution is 8%-12% in the step (1).
It is preferred that the mass percentage concentration of mixed solution is 10%.
Amount takes 10ml mixed solutions in a syringe respectively in step (1), then takes 20ml mixed solutions in another
In syringe.
The mass percentage concentration of pure collagen solution is 8%-12% in the step (2).It is preferred that the quality of pure collagen solution
Percentage concentration is 10%.
The pure collagen solutions of 10ml are measured in step (2) in syringe.
Conjugation electrostatic spinning is specially in the step (3)：Spinning environment temperature is 20-25 DEG C, and tumbler receives rotating speed
1200-1400rpm, conjugate spinning machine both sides add mixed solution and pure collagen solution respectively, and setting spinning voltage is 12-16kV,
Syringe needle is 12-15cm with the distance between tumbler is received, and the fltting speed for advancing pump is 0.10-0.15mm/h, will first be mixed molten
Liquid carries out spinning, prepares one-component layer, and then pure collagen solution continues spinning, prepares bi-component layer, until complete soln spins
Silk terminates, and obtains material.
It is preferred that spinning environment temperature is 25 DEG C.
After conjugate spinning terminates in step (2), need to be by 55-60 DEG C of vacuum dried process 120-130 minutes.
It is preferred that dried process temperature is 60 DEG C, the time is 120 minutes.
The size of the nanofiber body tissue rebulding material of PLGA and collagen composite can basis
Depending on the required size for repairing wound.I.e. the size of hernia repair material can according to it is required reparation wound size depending on.
Invention uses hexafluoroisopropanol (purity 99%) as solvent, by the polylactic-co-glycolic acid for adding HMW
Copolymer and both natural collagen and control proportioning, under certain environmental conditions, using the side of conjugation electrostatic spinning
Method, first carries out conjugate spinning using PLGA and collagen blend solution, then by conjugate spinning machine wherein
Individual shower nozzle is changed to pure fish glue original solution and carries out spinning, successfully electrospinning nanofibers body tissue rebulding material.
Beneficial effect
Compared to prior art, by changing the structure of traditional single repairing film, the structure of bi-component, this wound are changed to
Mouth repairing feed supplement, can provide a preferable structure and substitute natural cytoplasmic matrix.Because nano fibrous membrane have it is larger
Specific surface area and microcellular structure, and double-component layer is due to adding single collagen, and single collagen occurs dissolving change after treatment,
So that material has bigger hole, fibrocyte can be induced to adhere to skin corium, and produce some important extracellular bases
Matter composition, such as collagen and cell factor, so as to repair damaged tissues.Meanwhile, PLGA and glue
Protozoa compatibility is good, possesses good degradation capability, and the method is easy to operate, favorable repeatability, and material has good
Mechanical property, can reach good effect on hernia defect repairing.
Brief description of the drawings
Fig. 1 is prepared PLGA and collagen type complex nanometer fibrous tissue engineering material outward appearance
Figure；
Fig. 2 is that PLGA/pure collagen mass ratio is 60/40 nanofiber SEM in embodiment 1
Figure；
Fig. 3 is that PLGA/pure collagen mass ratio is that 60/40 nanofiber is done in embodiment 1
State (a) and the mechanical property picture by (b) after PBS immersions 24H；
Fig. 4 be in embodiment 2 PLGA/pure collagen mass ratio for 60/40 nanofiber water connects
Feeler figure；
It using L929 cells in PLGA/pure collagen mass ratio is 60/ that Fig. 5 is in embodiment 2
The cell SEM figures of growth 7 days are adhered on 40 nanofiber；
It using L929 cells in PLGA/pure collagen mass ratio is 60/ that Fig. 6 is in embodiment 3
The cell dyeing figure of growth 7 days is adhered on 40 nanofiber.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.In addition, it is to be understood that after the content for having read instruction of the present invention, people in the art
Member can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
Embodiment 1
(1) using hexafluoroisopropanol (purity 99.9%) as solvent；
(2) PLGA of 1.2g and the collagen of 0.8g are weighed respectively, are added in above-mentioned solvent,
Solution is placed on heatable magnetic stirring apparatus and is stirred overnight；The pure collagens of 1g are weighed again, are added in above-mentioned solvent, will
Solution is placed on heatable magnetic stirring apparatus and is stirred overnight, and the mass percentage concentration of mixed solution is 8%-12%.
Solution was taken out standby in (3) second days；
(4) electrospinning parameters are set and are respectively spinning voltage for 12-16kV, syringe needle is with the distance between tumbler is received
12-15cm, the fltting speed for advancing pump is 0.10-0.15mm/h, and spinning environment temperature is 25 DEG C.
The 10ml mixed solutions that prepare are taken respectively in a syringe, then takes 20ml mixed solutions injected in another
In device, the pure collagen solutions of 10ml are measured afterwards in a syringe.Conjugate spinning is carried out, mixed solution is first carried out into spinning,
One-component layer is prepared, then pure collagen solution continues spinning, prepares bi-component layer, until complete soln spinning terminates, obtains
Material, can obtain the good PLGA of fiber morphology with collagen mixing feed supplement, and porosity is 50-
60%.Fig. 2 is the scanning electron microscopic picture of the feed supplement, it can be observed that smooth continuous nanofibrous structures.This kind of feed supplement is entered
Row cell experiment, Fig. 3 is the nanofiber that PLGA/pure collagen mass ratio is 60/40 in embodiment 1
Dry state (a) and the mechanical property picture by (b) after PBS immersions 24H；Fig. 3 different colours curves represent same material respectively
The stress-strain diagram of different control groups, it is corresponding for black for same material removes the first sample strip stress in region at random
Strain curve, red is to take second stress-strain diagram of region samples bar at random on same material, by that analogy.
As shown in fig. 6, L929 cells have the effect of good growth adhesion in the feed supplement.
Embodiment 2
(1) using hexafluoroisopropanol (purity 99.9%) as solvent；
(2) PLGA of 1.2g and the collagen of 0.8g are weighed respectively, are added in above-mentioned solvent,
Solution is placed on heatable magnetic stirring apparatus and is stirred overnight；The pure collagens of 1g are weighed again, are added in above-mentioned solvent, will
Solution is placed on heatable magnetic stirring apparatus and is stirred overnight, and the mass percentage concentration of mixed solution is 8%-12%.
Solution was taken out standby in (3) second days；
(4) electrospinning parameters are set and are respectively spinning voltage for 12-16kV, syringe needle is with the distance between tumbler is received
12-15cm, the fltting speed for advancing pump is 0.10-0.15mm/h, and spinning environment temperature is 25 DEG C.
The 10ml mixed solutions that prepare are taken respectively in a syringe, then takes 20ml mixed solutions injected in another
In device, the pure collagen solutions of 10ml are measured afterwards in a syringe.Conjugate spinning is carried out, mixed solution is first carried out into spinning,
One-component layer is prepared, then pure collagen solution continues spinning, prepares bi-component layer, until complete soln spinning terminates, obtains
Material, can obtain the good PLGA of fiber morphology and collagen mixing material, and porosity is 50-
60%.The good mechanical properties of the material, possess more preferable mechanics and drawing effect after being soaked by PBS.Dry state is put down
4, row sample, first maximum stress of sample is 3.45Mpa, and second maximum stress of sample is 3.77Mpa, the 3rd sample
The maximum stress of product is 3.46Mpa, and the 4th maximum stress of sample is 4.84Mpa, and average maximum stress is 3.88Mpa.It is wet
State Duplicate Samples are also 4, wherein, first maximum stress of sample is 3.75Mpa, and the maximum stress of second sample is
3.97Mpa, the 3rd maximum stress of sample is 4.16Mpa, and the 4th maximum stress of sample is 3.83Mpa, average maximum
Stress is 3.93Mpa.The test of contact angle is carried out to the material simultaneously, as shown in figure 4, its water contact angle is 108.71 °, it is hydrophilic
Property is more good.
Embodiment 3
(1) hexafluoroisopropanol (purity 99.9%) is claimed as solvent；
(2) PLGA of 1.2g and the collagen of 0.8g are weighed respectively, are added in above-mentioned solvent,
Solution is placed on heatable magnetic stirring apparatus and is stirred overnight；The pure collagens of 1g are weighed again, are added in above-mentioned solvent, will
Solution is placed on heatable magnetic stirring apparatus and is stirred overnight, and the mass percentage concentration of mixed solution is 8%-12%.
Solution was taken out standby in (3) second days；
(4) electrospinning parameters are set and are respectively spinning voltage for 12-16kV, syringe needle is with the distance between tumbler is received
12-15cm, the fltting speed for advancing pump is 0.10-0.15mm/h, and spinning environment temperature is 25 DEG C.
The 10ml mixed solutions that prepare are taken respectively in a syringe, then takes 20ml mixed solutions injected in another
In device, the pure collagen solutions of 10ml are measured afterwards in a syringe.Conjugate spinning is carried out, mixed solution is first carried out into spinning,
One-component layer is prepared, then pure collagen solution continues spinning, prepares bi-component layer, until complete soln spinning terminates, obtains
Material, can obtain the good PLGA of fiber morphology and collagen mixing material, and porosity is 50-
60%.Fig. 5 is the cellscan electron microscopic picture of the dressing, it has also been observed that smooth continuous nanofibrous structures, while
It is observed that L929 cells grown on fibrous material adhesion it is all right, cell dyeing experiment is carried out again to this kind of material,
As shown in fig. 6, L929 cells grown on the fibrous material adhesion it is all right, uniformly sprawl.
Claims (10)
- It is 1. a kind of to be manually combined hernia paster, it is characterised in that：The hernia paster is PLGA and glue The compound nano-fiber material of original.
- 2. a kind of preparation method of hernia paster as claimed in claim 1 artificial compound, including：(1) PLGA and collagen are dissolved in solvent, configuration obtains mixed solution；(2) by pure collagen solution solvent, pure collagen solution is obtained；(3) above-mentioned mixed solution, pure collagen solution are carried out into conjugation electrostatic spinning as spinning solution, is obtained final product.
- 3. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：The step (1) mass ratio of PLGA and collagen is (58-62) in：(38-42).
- 4. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：The step (1), solvent is hexafluoroisopropanol in (2).
- 5. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：The step (1) mass percentage concentration of mixed solution is 8%-12% in.
- 6. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：In step (1) Amount takes 10ml mixed solutions in a syringe respectively, then takes 20ml mixed solutions in another syringe.
- 7. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：The step (2) mass percentage concentration of pure collagen solution is 8%-12% in.
- 8. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：In step (2) The pure collagen solutions of 10ml are measured in syringe.
- 9. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：The step (3) conjugation electrostatic spinning is specially in：Spinning environment temperature is 20-25 DEG C, and tumbler receives rotating speed 1200-1400rpm, and conjugation is spun Silk machine both sides add mixed solution and pure collagen solution respectively, and setting spinning voltage is 12-16kV, between syringe needle and reception tumbler Distance is 12-15cm, and the fltting speed for advancing pump is 0.10-0.15mm/h, and mixed solution first is carried out into spinning, prepares single group Layering, then pure collagen solution continuation spinning.
- 10. the preparation method of a kind of artificial compound hernia paster according to claim 2, it is characterised in that：In step (2) After conjugate spinning terminates, need to be by 55-60 DEG C of vacuum dried process 120-130 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710032246.0A CN106880874A (en) | 2017-01-16 | 2017-01-16 | A kind of manually compound hernia paster and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710032246.0A CN106880874A (en) | 2017-01-16 | 2017-01-16 | A kind of manually compound hernia paster and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106880874A true CN106880874A (en) | 2017-06-23 |
Family
ID=59176786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710032246.0A Pending CN106880874A (en) | 2017-01-16 | 2017-01-16 | A kind of manually compound hernia paster and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106880874A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110141687A (en) * | 2019-05-30 | 2019-08-20 | 四川大学 | A kind of hard soft tissue regeneration functionally gradient material (FGM) of guidance periodontal and preparation method thereof |
| CN116641160A (en) * | 2023-06-27 | 2023-08-25 | 东华大学 | Light elastic iron-cobalt-nickel/carbon-based wave-absorbing material, and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
| CN101773689A (en) * | 2010-03-29 | 2010-07-14 | 苑国忠 | Surgical repairing patch |
| CN102908667A (en) * | 2012-11-09 | 2013-02-06 | 无锡中科光远生物材料有限公司 | Preparation method for partially absorbable fiber film hernia patch |
| CN103132194A (en) * | 2011-11-30 | 2013-06-05 | 杨恩龙 | Orientation electro-spinning nanometer fiber spinning method and device thereof |
| CN105233339A (en) * | 2015-11-06 | 2016-01-13 | 东华大学 | Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent |
-
2017
- 2017-01-16 CN CN201710032246.0A patent/CN106880874A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
| CN101773689A (en) * | 2010-03-29 | 2010-07-14 | 苑国忠 | Surgical repairing patch |
| CN103132194A (en) * | 2011-11-30 | 2013-06-05 | 杨恩龙 | Orientation electro-spinning nanometer fiber spinning method and device thereof |
| CN102908667A (en) * | 2012-11-09 | 2013-02-06 | 无锡中科光远生物材料有限公司 | Preparation method for partially absorbable fiber film hernia patch |
| CN105233339A (en) * | 2015-11-06 | 2016-01-13 | 东华大学 | Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110141687A (en) * | 2019-05-30 | 2019-08-20 | 四川大学 | A kind of hard soft tissue regeneration functionally gradient material (FGM) of guidance periodontal and preparation method thereof |
| CN116641160A (en) * | 2023-06-27 | 2023-08-25 | 东华大学 | Light elastic iron-cobalt-nickel/carbon-based wave-absorbing material, and preparation method and application thereof |
| CN116641160B (en) * | 2023-06-27 | 2024-06-04 | 东华大学 | Light elastic iron-cobalt-nickel/carbon-based wave-absorbing material, and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11696972B2 (en) | Process for the production of a hybrid structure consisting of coupled silk fibroin microfibers and nanofibers, hybrid structure thus obtained and its use as implantable medical device | |
| Yaari et al. | Wet spinning and drawing of human recombinant collagen | |
| EP3351376B1 (en) | Silk biomaterials and methods of use thereof | |
| CN101507843B (en) | Multi-purpose surgery biology patching material | |
| Sanhueza et al. | One-step electrospun scaffold of dual-sized gelatin/poly-3-hydroxybutyrate nano/microfibers for skin regeneration in diabetic wound | |
| EP2465472B1 (en) | Method for producing a silk nanofiber nerve conduit | |
| CN106029361A (en) | Tissue substitute multilayer matrix and uses thereof | |
| CN106693059A (en) | Composite tissue repair patch and preparation method and application thereof | |
| CN105999419A (en) | Bionical type absorbable dura mater patch and preparation method and application thereof | |
| CN101773689B (en) | Surgical repairing patch | |
| Bazbouz et al. | A UV-cured nanofibrous membrane of vinylbenzylated gelatin-poly (ɛ-caprolactone) dimethacrylate co-network by scalable free surface electrospinning | |
| CN105919694A (en) | Multi-layer electrospun membrane and use thereof | |
| CN106668954A (en) | Antibacterial cation-modified absorbable pachymeninx repair material and preparation method and application thereof | |
| CN109260520B (en) | Multifunctional dura mater repair material based on bionic design and preparation method thereof | |
| CN106390196A (en) | Preparation method of nanofiber nerve tissue engineering scaffold | |
| CN105887327B (en) | A kind of composite nano-fiber membrane and preparation method thereof | |
| CN107737364A (en) | A kind of wound dressing and preparation method thereof | |
| CN106880874A (en) | A kind of manually compound hernia paster and preparation method thereof | |
| CN110354316A (en) | A kind of compound tendon Antiadhesive film and the preparation method and application thereof | |
| CN108295319A (en) | A kind of hydrophilic composite material and preparation method of medical nano fiber reinforcement type and purposes | |
| KR100491705B1 (en) | Wound dressing of silk fibroin nanofibers nonwoven and its preparation | |
| CN109943974B (en) | Preparation method of nerve conduit material based on polyhydroxyalkanoate/gelatin electrospun nanofiber | |
| CN113046917B (en) | Electrostatic spinning collagen membrane and preparation method thereof | |
| CN103741262B (en) | A kind of chitosan nano fibrous membrane for cerebral dura mater or Broken dura remedy and preparation method thereof and application | |
| CN115804867A (en) | Fibrosis type III collagen nano-film, preparation method and application in skin regeneration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170623 |