CN106860493B - Application of kiwi fruit peel extract in preparation of fatty acid synthase inhibitor - Google Patents

Application of kiwi fruit peel extract in preparation of fatty acid synthase inhibitor Download PDF

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CN106860493B
CN106860493B CN201710229079.9A CN201710229079A CN106860493B CN 106860493 B CN106860493 B CN 106860493B CN 201710229079 A CN201710229079 A CN 201710229079A CN 106860493 B CN106860493 B CN 106860493B
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acid synthase
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梁妍
吴昊
罗笛
高萱
任景萍
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Capital University of Physical Education and Sports
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Abstract

The invention discloses a kiwi fruit peel extract, a preparation method thereof and application thereof in inhibiting activity of fatty acid synthase. The invention provides a preparation method of a kiwi fruit peel extract, which comprises the following steps: 1) sequentially removing pulp and degreasing kiwi fruits to obtain a degreased kiwi fruit peel mixture; 2) uniformly mixing the degreased kiwi fruit peel mixture obtained in the step 1) with an organic solvent A aqueous solution to obtain a mixed solution; the organic solvent A aqueous solution is any one of ethanol aqueous solution, propanol aqueous solution, methanol aqueous solution and acetone aqueous solution; 3) carrying out ultrasonic extraction on the acidified mixed liquid obtained in the step 2), and collecting ultrasonic products to obtain the kiwi fruit peel extract. Experiments prove that the extract capable of inhibiting the activity of the fatty acid synthase can be prepared from the kiwi fruit, and is a natural, plant-derived and non-toxic fatty acid synthase inhibitor.

Description

Application of kiwi fruit peel extract in preparation of fatty acid synthase inhibitor
Technical Field
The invention relates to the technical field of biology, in particular to a kiwi fruit peel extract, a preparation method thereof and application thereof in inhibiting activity of fatty acid synthase.
Background
Fatty acid synthases are the most important enzymes in the synthesis of fats in biological cells. Studies of fatty acid synthase in poultry by Tianxi et al found that there is a very high positive correlation between the fat level in the abdominal cavity of poultry and the activity of fatty acid synthase, and proposed that controlling the activity of fatty acid synthase is an effective method for controlling the fat level of animals (Tianxi, 1994. regulation of body fat level and fatty acid synthase activity of animals. chemistry of life, 14, (1): 184; Tianweixi et al, 1996. relationship between body fat level and liver fatty acid synthase activity of layers in different growth stages. journal of Biochemistry, 12(2): 234-. In 2000, results of studies on mouse fatty acid synthase by Loftus et al, university of Hopkins, demonstrated that inhibition of fatty acid synthase caused accumulation of malonyl-CoA, while inhibition of the expression of feeding-related hypothalamic neuropeptide Y, resulted in decreased appetite and weight loss without affecting the mobility of mice. Loftus et al indicate that fatty acid synthase can be a potential therapeutic target for weight control (Loftus. T.M.et al, 2000.Science,288(5475): 2379-. In addition, many studies have found that the activity and expression of Fatty Acid Synthase (FAS) show extremely low levels in almost all non-diseased adult tissues, while its activity is significantly increased and expression is significantly up-regulated in many types of cancer tissues, such as rectal cancer, breast cancer, prostate cancer, endometrial cancer, ovarian cancer, lung cancer, liver cancer, and the like. Meanwhile, it has been found that cerulenin, an inhibitor of FAS, can kill cancer cells and inhibit the growth of xenograft tumors, and other FAS inhibitors such as cerulenin derivative C75, β -lactone orlistat, EGCG and other flavonoids of natural origin, etc. and antibiotic triclosan have also been confirmed to inhibit the growth of tumor cells by inducing apoptosis. The above results indicate that fatty acid synthase may be a potential dual target for the treatment of obesity and tumors. Therefore, the development and development of inhibitors of fatty acid synthase would have great practical significance and potential for development.
Actinidia chinensis Planch is a deciduous woody vine plant of Actinidia of Actinidiaceae (Actinidiaceae), and has edible fruit and unique flavor. The kiwi fruit contains organic substances such as actinidine, proteolytic enzyme, tannin pectin, saccharides and the like, trace elements such as calcium, potassium, selenium, zinc, germanium and the like, 17 amino acids required by a human body, and also contains abundant vitamin C, glucose, fructose, citric acid, malic acid and fat, so that the kiwi fruit is a new health food and has high nutritional value. Chinese patent publication No. CN105230896A discloses a fruit weight-reducing tea with kiwi fruit juice as a component. The Chinese patent publication No. CN105520007A discloses a dendrobium-kiwi fruit compound beverage and a preparation method thereof, and the compound beverage can effectively supplement nutrient factors necessary for human bodies and maintain dietary balance. Also, as disclosed in chinese patent publication No. CN105361036A, a process for producing cordyceps militaris kiwi enzyme with weight-reducing effect is invented. The health food containing kiwi fruit invented in the above patents is based on the traditional efficacy of kiwi fruit.
Disclosure of Invention
The invention aims to provide a preparation method of a kiwi fruit peel extract.
The method provided by the invention comprises the following steps:
1) degreasing kiwi fruit peel to obtain degreased kiwi fruit peel;
2) ultrasonically extracting the degreased kiwi fruit peel obtained in the step 1), and collecting supernatant to obtain the kiwi fruit peel extract.
In the step 1), the degreasing treatment is to perform reflux extraction on the kiwi fruit peel by using an organic solvent, and filter and remove the organic solvent to obtain the degreased kiwi fruit peel;
or the ratio of the kiwi fruit peel to the organic solvent is 1g: (6-20) ml or 1g (10-20) ml;
or the reflux extraction temperature is 30-90 deg.C, and the time is 1-12 hr or 1-7 hr;
or in the step 2), the extracting solution adopted by the ultrasonic extraction is an ethanol water solution;
the ultrasonic extraction is to perform ultrasonic extraction on the degreased kiwi fruit peel by using an ethanol water solution to obtain an ultrasonic product; filtering and collecting the supernatant of the ultrasonic product;
the ultrasonic power is more than 100W; the ultrasonic mode is that the ultrasonic is carried out for 3 times in sequence, and the ultrasonic time is 10-30min each time. (the ultrasonic treatment is respectively carried out for 3 times in sequence, after ultrasonic extraction liquid is collected after each ultrasonic treatment is finished, a new solution is replaced, the ultrasonic treatment is carried out again for three times in total.)
In the method, in the step 2), the volume percentage content of the ethanol aqueous solution is 20-95%.
In the above method, in the step 2), after the step of collecting the supernatant, the method further comprises the following steps: removing ethanol in the supernatant, extracting the supernatant with ethyl acetate, collecting an organic phase, and removing the ethyl acetate in the organic phase to obtain the kiwi fruit peel extract.
In the method, the volume ratio of the supernatant to the ethyl acetate is 1 (1-4).
In the above method, the removing of ethanol in the supernatant or the removing of ethyl acetate in the organic phase is performed by concentration under reduced pressure;
the temperature of the reduced pressure concentration is 35-55 ℃, and the pressure is 0.01-0.08 MPa.
In the above method, step 1) further comprises the following steps before the degreasing treatment: and crushing the kiwi fruit peel, sieving the crushed kiwi fruit peel with a 20-mesh sieve, and collecting a filtered product.
In the above method, the organic solvent is petroleum ether.
The kiwi fruit peel extract prepared by the above method is also within the protection scope of the present invention.
The application of the kiwi fruit peel extract in inhibiting fatty acid synthase or preparing products for losing weight or preparing products for resisting cancer is also within the protection scope of the invention.
It is another object of the invention to provide a product that inhibits fatty acid synthase.
The active ingredient of the product for inhibiting fatty acid synthase provided by the invention is the kiwi fruit peel extract.
Experiments prove that compared with the prior art, the invention has the following advantages:
1. the invention can prepare an extract which can inhibit the activity of fatty acid synthase from the peel of the kiwi fruit, and the extract is a natural, plant-derived and non-toxic fatty acid synthase inhibitor;
2. the kiwi fruit peel extract obtained by the invention can obviously inhibit the activity of fatty acid synthase, and the activity of the kiwi fruit peel extract is equivalent to that of the currently known fatty acid synthase inhibitors of natural sources, such as EGCG and the like;
3. because the strength of the activity of the fatty acid synthase directly influences the amount of fat synthesized in biological cells and the capability of tumor cells for synthesizing fatty acid, the activity of the fatty acid synthase is inhibited, so that the process of synthesizing fat in the biological cells can be weakened, and the fat content can be controlled or even reduced; on the other hand, the ability of synthesizing fatty acid of tumor cells is reduced, so that the growth of the tumor cells can be inhibited; therefore, the extract of the invention is used as an active ingredient to prepare weight-losing drugs or anticancer drugs of various dosage forms, which has the characteristics of obvious efficacy and activity, clear action mechanism, definite target spot and the like and meets the requirement of pharmaceutical preparation development;
4. because the kiwi fruit is a traditional edible plant resource, the extract prepared from the kiwi fruit is used as a raw material to prepare health-care food, functional cosmetics, common food and the like for losing weight or resisting tumors, and meets the national requirements of the restriction and regulation on the raw materials of the health-care food and the common food, so the extract for inhibiting the activity of the fatty acid synthase has wider application and development prospects.
In a word, the invention not only enriches the sources of pure natural fatty acid synthase inhibitors, but also develops new efficacy and application value of the kiwi fruit plants with regional characteristics and resource advantages.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The following examples are further illustrative of the present invention and are not meant to be any limitation of the present invention. Various alternatives and modifications can be devised by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Example 1 obtaining and testing of Kiwi fruit Peel extract
1. Obtaining and detecting kiwi fruit peel extract
1) Crushing the pulp-removed peel of kiwi fruit, sieving with a 20-mesh sieve, collecting fine powder after filtration, and performing reflux extraction with petroleum ether at the temperature of 30 ℃ (the proportion of the fine powder to the petroleum ether is 1g: 10ml) for 7 hours, filtering and removing petroleum ether to obtain degreased kiwi fruit peel;
2) soaking the degreased kiwi fruit peel obtained in the step 1) in 20% ethanol water solution by volume, performing ultrasonic extraction (XH-2008DE ultrasonic extractor, Beijing auspicin research and development Co., Ltd.) at normal temperature (25 ℃), performing ultrasonic treatment for 3 times with ultrasonic power of 200W, performing ultrasonic treatment for 30min each time, filtering the liquid after ultrasonic treatment by filter paper, and collecting and combining the liquid parts subjected to ultrasonic treatment for 3 times to obtain an extract. (3 times of ultrasound are carried out successively, each time of ultrasound is an extraction process, and the total 3 times of ultrasound is to ensure that the extraction is more sufficient), filtering and collecting the supernatant (namely an ultrasound extract and filtered liquid) of an ultrasound product by using filter paper, and concentrating under reduced pressure at the temperature of 35 ℃ and the pressure of 0.08MPa until no alcohol smell exists to obtain a crude extract;
3) adding ethyl acetate with the volume of 4 times of that of the crude extract obtained in the step 2), extracting at room temperature (25 ℃) and standing for natural layering, collecting an organic phase, concentrating under reduced pressure at the temperature of 35 ℃ and the pressure of 0.08MPa until the solvent is completely volatilized, and drying to constant weight to obtain the kiwi fruit peel extract A.
2. Detection of kiwi fruit peel extract
1) Preparation of Fatty Acid Synthase (FAS)
Crude extraction of fatty acid synthase FAS: fresh chicken liver was weighed and added at 1:1.8(g/mL) to extraction buffer (0.1mol/L KH) at 4 deg.C2PO4-K2HPO4，1mmol/L EDTA，1mmol/L DTT，0.07mol/L KHCO3pH 8.0), homogenization for 50 seconds. The homogenate volume was measured and centrifuged at 8000r/min at 4 ℃ for 15 min. Supernatant fluidThe warp was filtered through a cloth and the volume was measured and centrifuged at 35000r/min at 4 ℃ for 30 min. Filtering the supernatant, subpackaging in low temperature resistant plastic bottles at-20 deg.C overnight, and storing the rest at-80 deg.C for use.
Ammonium sulfate is precipitated step by step: the frozen supernatant obtained by high-speed centrifugation is thawed in a water bath, and then placed in an ice box, and saturated ammonium sulfate (containing 1mmol/L DTT) with pH value of 7.0 at 4 ℃ is added dropwise to 25% saturation according to one third volume amount under the magnetic stirring state. Stirring for 10min, centrifuging at 10000r/min speed and 4 deg.C for 15min, and removing precipitate. Samples were taken to determine FAS activity in the supernatant.
Then, one quarter volume of saturated ammonium sulfate (containing 1mmol/L DTT) with pH7.0 at 4 ℃ of the clear liquid is dripped to 40% saturation under stirring, and after stirring for 10min, the mixture is centrifuged at 10000r/min for 15 min. If the supernatant was assayed to contain no or little FAS activity, the supernatant was discarded and the pellet was retained.
Ion exchange chromatography: DEAE cellulose DE52 was treated with 4 ℃ buffer A (5mmol/L KH)2PO4-K2HPO4Containing 1mmol/L EDTA, 1mmol/L DTT, pH 7.0). And (3) after the precipitation is drained, dissolving the precipitation in a proper amount of buffer solution A at 4 ℃, and loading the sample when the measured conductivity value is not more than 5.5 ms/cm. After loading, washing with buffer A at 4 deg.C until the absorption of hetero protein is 0, and sequentially washing with 50mmol/L, 160mmol/L, 250mmol/L, and 1mol/L KH at 4 deg.C2PO4-K2HPO4Buffer (both containing 1mmol/L EDTA, 1mmol/L DTT, pH 7.0). And respectively collecting elution peaks, and measuring the volume, the protein content and the FAS activity. The chromatographic process was monitored using an LKB protein monitor and recorder.
Affinity chromatography: blue Sepharose 6B resin with 4 ℃ buffer B (50mmol/L KH)2PO4-K2HPO4Containing 1mmol/L EDTA, 1mmol/L DTT, pH 7.0). Subjecting DEAE to chromatography at 250mmol/L KH2PO4-K2HPO4And (4) eluting the collected liquid with a buffer solution and loading. After loading, the sample was washed with buffer B at 4 ℃ and then eluted with buffer B containing 0.35mol/L and 2mol/L NaCl at 4 ℃. And respectively collecting elution peaks, and measuring the volume, the protein content and the FAS activity.
Concentrating and storing: press under the stirring stateTwo-thirds of the volume of the eluate pool containing 0.35mol/L NaCl buffer B was slowly added to a 4 ℃ saturated ammonium sulfate solution to 40% saturation to precipitate FAS. Stirring for 10min, and centrifuging at 10000r/min speed and 4 deg.C for 15 min. Discarding supernatant, draining the precipitate, dissolving in small amount of storage buffer (0.1mol/L KH)2PO4-K2HPO41mmol/L EDTA, 10mmol/L DTT, 20% glycerol, pH7.0), making FAS concentration at about 10mg/mL, taking a small amount of sample to determine protein concentration and FAS activity, subpackaging the rest, standing overnight at-20 deg.C, and freezing at-80 deg.C for storage to obtain Fatty Acid Synthase (FAS).
All the steps of the separation and purification process are carried out at the low temperature of 4-8 ℃. The fatty acid synthase of the final sample is detected as a single band by polyacrylamide gel electrophoresis, and the molecular weight is 270 kD.
2) Inhibition of fatty acid synthase by kiwi fruit peel extract
Dissolving 10mg of kiwi fruit peel extract A obtained from 1 in 200mL of DMSO to obtain a test sample solution; then diluting the solution into sample solutions to be detected with different concentrations.
Fatty acid synthase activity was determined by standard activity-determining methods using acetyl-CoA, malonyl-CoA A, NADPH as substrates (W.X.Tian et al, 1985.J.biol.chem.,260(20): 11375-11387; Tianweixi et al, 1996. relationship between body fat levels and hepatic fatty acid synthase activity in layers of different growth phases. J.Biochem., 12(2): 234-236).
A solution of a sample to be tested (concentration: 1, 2, 5, 10, 20, 50, 100, 200. mu.g/mL) was added to a system for measuring the activity (formulation: 100mM phosphate buffer solution of pH7.0 containing 3mM acetyl-CoA, 10mM malonyl-CoA, 35mM NADPH, concentration: 100 mM), and the total volume: 2mL), incubated at 37 ℃ and then added with an amount (15U/mg per enzyme activity) of fatty acid synthase FAS-initiated reaction obtained from 1), and the enzyme activity was measured as Ai. The enzyme activity measured by the sample-added solvent control is A0，Ai/A0This is the Remaining Activity of FAS (Remaining Activity, r.a.). The smaller this value, the stronger the inhibition of FAS by the sample. Inhibition as determined by this method is often the caseThe following is reversible, meaning that the binding of the inhibitor to the enzyme is non-covalent.
The extent to which Fatty Acid Synthase (FAS) activity is inhibited is determined by IC50(semi-inhibitory concentration) and the calculation method comprises the following steps: determining the fatty acid synthase activity value of the substrate by using only the corresponding extraction solvent as a control, and using A as the value0Expressed and set to 100%. Adding fatty acid synthase inhibitor extract into substrate as experimental group, and measuring the value of fatty acid synthase activity as A0At 50%, the concentration of inhibitor is IC50The value is obtained. The smaller the value, the stronger the inhibition of FAS by the sample.
After the kiwi fruit peel extracts with the concentrations of 1, 2, 5, 10, 20, 50, 100 and 200 mug/mL are respectively added into the tested living systems, the measured values of the relative activities of the fatty acid synthase are respectively as follows: 99%, 97%, 87%, 75%, 66%, 38%, 21% and 13%.
The results indicate the half Inhibitory Concentration (IC) of the kiwi fruit peel extract A against fatty acid synthase50) It was 33.8. mu.g/mL.
From the above results, it can be shown that the extract A of the pericarp of Actinidia chinensis planch has a strong inhibitory effect on the activity of fatty acid synthase, and the required effective dose is very low (less than 50 μ g/mL).
Example 2 obtaining and testing of Kiwi fruit Peel extract
1. Obtaining and detecting kiwi fruit peel extract
1) Crushing the pulp-removed peel of kiwi fruit, sieving the crushed pulp-removed peel with a 20-mesh sieve, collecting fine powder after filtration, and performing reflux extraction with petroleum ether at the temperature of 90 ℃ (the proportion of the fine powder to the petroleum ether is 1g: 15ml) for 3 hours, filtering to remove petroleum ether to obtain degreased kiwi fruit peel;
2) soaking the degreased kiwi fruit peel obtained in the step 1) in 95% ethanol aqueous solution by volume percentage, performing ultrasonic extraction (XH-2008DE ultrasonic extractor, Beijing Sunjin swan science and technology development Co., Ltd.) at normal temperature (25 ℃), performing ultrasonic treatment for 3 times in sequence with ultrasonic power of 200W and ultrasonic power of 200W, performing ultrasonic treatment for 30min each time, filtering the liquid after ultrasonic treatment by filter paper, collecting and combining the liquid parts after 3 times of ultrasonic treatment to obtain the extract. Concentrating under reduced pressure at 55 deg.C and 0.01MPa until no alcohol smell is produced (removing ethanol) to obtain crude extractive solution;
3) adding 1 time volume of ethyl acetate into the crude extract obtained in the step 2), extracting at room temperature (25 ℃) and standing for natural layering, collecting an organic phase, concentrating under reduced pressure at 55 ℃ and 0.01MPa until the solvent is completely volatilized, and drying to constant weight to obtain the kiwi fruit peel extract B.
2. Detection of kiwi fruit peel extract
1) Preparation of Fatty acid synthase (Fatty acid synthase)
Same as 1) of 2 of example 1.
2) Inhibition of fatty acid synthase by kiwi fruit peel extract
The process was the same as 2) of 2 of example 1,
the results indicate the half Inhibitory Concentration (IC) of the kiwi fruit peel extract B for fatty acid synthase50) Was 34.0. mu.g/mL.
After the kiwi fruit peel extracts with the concentrations of 1, 2, 5, 10, 20, 50, 100 and 200 mug/mL are respectively added into the tested living systems, the measured values of the relative activities of the fatty acid synthase are respectively as follows: 98%, 95%, 90%, 77%, 62%, 35%, 17%, 11%.
The kiwi fruit peel extract B has strong inhibition effect on the activity of fatty acid synthase, and the required effective dose is very low (less than 50 mu g/mL).
Example 3 obtaining and testing of Kiwi fruit pericarp extract
1. Obtaining and detecting kiwi fruit peel extract
1) Crushing the pulp-removed peel of kiwi fruit, screening the crushed pulp-removed peel by a 20-mesh screen, collecting fine powder after filtration, and performing reflux extraction by using petroleum ether at the temperature of 60 ℃ (the proportion of the fine powder to the petroleum ether is 1g: 20ml) of the mixture is degreased for 1 hour, and petroleum ether is removed by filtration to obtain degreased kiwi fruit peel;
2) soaking the degreased kiwi fruit peel obtained in the step 1) in 50% ethanol aqueous solution by volume percentage, performing ultrasonic extraction at normal temperature (25 ℃), sequentially performing ultrasonic treatment for 3 times with ultrasonic power of 200W for 30min each time, filtering the ultrasonic liquid by filter paper, collecting and combining the liquid parts subjected to ultrasonic treatment for 3 times to obtain an extract. Concentrating under reduced pressure at 55 deg.C and 0.1MPa until no alcohol smell exists to obtain crude extractive solution;
3) adding 2 times volume of ethyl acetate into the crude extract obtained in the step 2), extracting at room temperature (25 ℃) and standing for natural layering, collecting an organic phase, concentrating under reduced pressure at 55 ℃ and 0.1MPa until the solvent is completely volatilized, drying to constant weight, and drying to obtain the kiwi fruit peel extract C.
2. Detection of kiwi fruit peel extract
1) Preparation of Fatty acid synthase (Fatty acid synthase)
Same as 1) of 2 of example 1.
2) Inhibition of fatty acid synthase by kiwi fruit peel extract
The process was the same as 2) of 2 of example 1,
after the kiwi fruit peel extracts with the concentrations of 1, 2, 5, 10, 20, 50, 100 and 200 mug/mL are respectively added into the tested living systems, the measured values of the relative activities of the fatty acid synthase are respectively as follows: 95%, 94%, 88%, 73%, 65%, 33%, 15%, 12%.
The results indicate the half Inhibitory Concentration (IC) of the kiwi fruit peel extract C for fatty acid synthase50) 35.7. mu.g/mL.
The result shows that the kiwi fruit peel extract C has strong inhibition effect on the activity of fatty acid synthase, and the required effective dose is very low (less than 50 mu g/mL).
Control group:
EGCG was added to a phosphate buffer containing acetyl-CoA and malonyl-coenzyme A, NADPH at concentrations of 5, 10, 20, 40, 80 and 160. mu.g/mL, respectively, and the mixture was incubated at 37 ℃ and then FAS having an enzymatic activity of 10-15U/mg per unit volume was added to initiate the reaction, and the enzymatic activity was determined to be Ai. The extent to which Fatty Acid Synthase (FAS) activity is inhibited is determined by IC50(semi-inhibitory concentration) and the calculation method comprises the following steps: by adding only to the substrateA control was added to the corresponding extraction solvent, and the fatty acid synthase activity value was measured and expressed as A0 and set to 100%. The test group added with fatty acid synthase inhibitor extract solution has fatty acid synthase activity value of 50% of A0, and the concentration of inhibitor is IC50The value is obtained. The smaller the value, the stronger the inhibition of FAS by the sample.
Using the above method, an experiment was conducted using the fatty acid synthase inhibitor EGCG (Epigallocatechin gallate, available from Sigma Co.) whose half Inhibitory Concentration (IC) against fatty acid synthase was50) It was 25. mu.g/mL.

Claims (1)

1. The application of the kiwi fruit peel extract in the preparation of the fatty acid synthase inhibitor is characterized in that: the preparation method of the kiwi fruit peel extract comprises the following steps:
1) crushing the kiwi fruit peel, screening the crushed kiwi fruit peel by a 20-mesh screen, and collecting a filtered product; then carrying out reflux extraction on the filtered product by using petroleum ether, and filtering to remove the petroleum ether to obtain degreased kiwi fruit peel;
the mixture ratio of the filtered product to the petroleum ether is 1g (10-20) ml;
the temperature of the reflux extraction is 30-90 ℃, and the time is 1-7 hours;
2) ultrasonically extracting the degreased kiwi fruit peel obtained in the step 1) by using an ethanol water solution with the volume percentage of 20-95%, and collecting supernatant to obtain a kiwi fruit peel extract;
the ultrasonic power is more than 100W; the ultrasonic extraction mode is that ultrasonic extraction is carried out for 3 times in sequence, and the ultrasonic time is 10-30min each time;
3) removing ethanol in the supernatant obtained in the step 2) by vacuum concentration, extracting the supernatant by using ethyl acetate, collecting an organic phase, and finally removing the ethyl acetate in the organic phase by vacuum concentration to obtain a kiwi fruit peel extract;
the volume ratio of the supernatant to the ethyl acetate is 1 (1-4);
the temperature of the reduced pressure concentration is 35-55 ℃, and the pressure is 0.01-0.08 MPa.
CN201710229079.9A 2017-04-10 2017-04-10 Application of kiwi fruit peel extract in preparation of fatty acid synthase inhibitor Active CN106860493B (en)

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