CN106860435A - A kind of mao of plain rice tree extract and preparation method thereof and preparing the application in preventing and treating bacterium infection medicine - Google Patents
A kind of mao of plain rice tree extract and preparation method thereof and preparing the application in preventing and treating bacterium infection medicine Download PDFInfo
- Publication number
- CN106860435A CN106860435A CN201611186026.5A CN201611186026A CN106860435A CN 106860435 A CN106860435 A CN 106860435A CN 201611186026 A CN201611186026 A CN 201611186026A CN 106860435 A CN106860435 A CN 106860435A
- Authority
- CN
- China
- Prior art keywords
- plain rice
- tree extract
- rice tree
- hair
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 38
- 235000009566 rice Nutrition 0.000 title claims abstract description 38
- 239000000284 extract Substances 0.000 title claims abstract description 31
- 239000003814 drug Substances 0.000 title claims abstract description 21
- 241000894006 Bacteria Species 0.000 title claims abstract description 18
- 208000015181 infectious disease Diseases 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000000605 extraction Methods 0.000 title description 5
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 38
- 150000001875 compounds Chemical class 0.000 claims abstract description 22
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 14
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 42
- YMWUJEATGCHHMB-UHFFFAOYSA-N dichloromethane Natural products ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 32
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 24
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 8
- 238000010898 silica gel chromatography Methods 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 241000785753 Flueggea Species 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000002034 butanolic fraction Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- -1 dichloromethane Alkane Chemical class 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims description 2
- 238000003018 immunoassay Methods 0.000 claims 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims 1
- 229940125904 compound 1 Drugs 0.000 abstract description 8
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 abstract description 7
- 229960003085 meticillin Drugs 0.000 abstract description 7
- 241000244317 Tillandsia usneoides Species 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 230000000844 anti-bacterial effect Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 229940125782 compound 2 Drugs 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000002815 broth microdilution Methods 0.000 description 3
- 229940126214 compound 3 Drugs 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229930004069 diterpene Natural products 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 206010041925 Staphylococcal infections Diseases 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 108010059993 Vancomycin Proteins 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 150000004141 diterpene derivatives Chemical class 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 150000002148 esters Chemical group 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 238000002114 high-resolution electrospray ionisation mass spectrometry Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 208000015339 staphylococcus aureus infection Diseases 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/05—Phenols
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
- C07C37/70—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment
- C07C37/82—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment by solid-liquid treatment; by chemisorption
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C39/00—Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring
- C07C39/23—Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring polycyclic, containing six-membered aromatic rings and other rings, with unsaturation outside the aromatic rings
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The application in preventing and treating bacterium infection medicine is being prepared the invention discloses a kind of mao of plain rice tree extract, the hair plain rice tree extract is the one kind in following structural formula.The present invention additionally provides the preparation method of the hair plain rice tree extract, the present invention is to extract the compound for obtaining structure as shown below formula from vegetable hair plain rice tree first.Wherein compound 1 is new compound, and the hair plain rice tree extract that the present invention is provided has certain inhibition, can be applied to prepare the medicine for preventing and treating bacterium infection to methicillin-resistant staphylococcus aureus and/or staphylococcus aureus.
Description
Technical field
The invention belongs to active ingredient of Chinese herbs and technical field of pharmaceuticals, more particularly, to a kind of hair plain rice tree extract
And preparation method thereof prepare prevent and treat bacterium infection medicine in application.
Background technology
After finding penicillin from nineteen twenty-eight Fleming, antibiotic turns into clinical mainstay, but, also bring bacterium resistance to
The problem of the property of medicine.Hospital Statistics in 2014 find, infect the patient of methicillin-resistant staphylococcus aureus than do not infect intolerant to
The mortality of medicine staphylococcus aureus wants high by 64%.Drug resistance has turned into the significant problem of serious harm human health.
Hair plain rice tree (Flueggea acicularis) is Euphorbiaceae plain rice Pterostyrax plant, is the endemic plant of China.
China is distributed mainly on the province such as Hubei, Sichuan, Yunnan.The compound mainly found from the category other plant includes diterpene, biology
Alkali etc..Its activity is mainly seen in anticancer and antiviral both sides report.
The report of its diterpene compound antimicrobial agent is had no in existing technology.
The content of the invention
It is an object of the invention to according to deficiency of the prior art, there is provided a kind of hair plain rice tree extract.
Another object of the present invention is to provide a mao preparation method for plain rice tree extract.
The application in preventing and treating bacterium infection medicine is being prepared it is still another object of the present invention to provide hair plain rice tree extract.
The purpose of the present invention is achieved through the following technical solutions：
The invention provides application of a kind of mao of plain rice tree extract in preparation prevents and treats bacterium infection medicine, the hair is white
Meal tree extract is the one kind in following chemical formula：
Wherein, compound 1 is noval chemical compound, and compound 2 and 3 is the compound of known structure.
Preferably, the hair plain rice tree extract is to prepare preventing and treating methicillin-resistant staphylococcus aureus and/or gold
Application in staphylococcus aureus infection medicine.
It is highly preferred that the hair plain rice tree extract with suppression methicillin-resistant staphylococcus aureus is：
It is highly preferred that the hair plain rice tree extract with suppression staphylococcus aureus is：
Preferably, the medicine includes pharmaceutically acceptable carrier or auxiliary material.
Present invention simultaneously provides a kind of new compound, the new compound is to extract to obtain from hair plain rice tree, on
The structural formula for stating compound is as follows：
The present invention also provides a kind of preparation method of new hair plain rice tree extract, and the preparation method is by hair plain rice tree
(Flueggea acicularis) complete stool, dries, and crushes, and is extracted three times with 95% ethanol room temperature, 24 hours every time, merges dense
Contracting liquid, respectively with ethyl acetate, extracting n-butyl alcohol obtains ethyl acetate portion and n-butanol fraction, then by ethyl acetate portion
Lease making crosses silica gel column chromatography, dichloromethane：Methyl alcohol is respectively 200 according to percentage:1、100:1、50:1、20:1 and 10:1 gradient
Afford six cuts, again by repeatedly isolated, the hair plain rice tree extract is in following chemical formula to cut
Kind：
Further, it is by dichloromethane：Methyl alcohol according to respectively according to percentage be 200:1、100:1、50:1、20:1
Cut D is obtained after carrying out gradient elution, by cut D isolated hair plain rice tree extracts repeatedly.
Shown based on broth dilution method antibacterial activity result, the compound 1 that extraction is obtained is to methicillin-resistant staphylococcus
Staphylococcic minimum inhibitory concentration (MIC value) is 62.5 μM, compound 2 and the 3 pairs of staphylococcus aureuses it is minimum antibacterial dense
Degree (MIC value) is 62.5 μM.This shows that hair plain rice tree extract 1-3 can be used to prepare the medicine for preventing and treating bacterium infection.
Compared with prior art, the present invention has advantages below and beneficial effect：
The application in preventing and treating bacterium infection medicine is being prepared the invention discloses a kind of mao of plain rice tree extract, while open
A kind of hair plain rice extract of novel structure, the present invention is to extract to obtain the hair plain rice tree first from vegetable hair plain rice tree
Extract, the hair plain rice tree extract that the present invention is provided is to methicillin-resistant staphylococcus aureus and/or Staphylococcus aureus
Bacterium has certain inhibition, can be applied to prepare the medicine for preventing and treating bacterium infection.
Specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment, but embodiment does not do any type of to the present invention
Limit.Unless stated otherwise, the reagent for using of the invention, method and apparatus are the art conventional reagent, method and apparatus.
Unless stated otherwise, agents useful for same of the present invention and material are purchased in market.
Embodiment 1
The method of prepare compound 1-3 from hair plain rice tree (Flueggea acicularis)：
Hair plain rice tree (Flueggea acicularis) complete stool, dry, crush, with 95% ethanol room temperature extraction three times, often
Secondary 24 hours, merge concentrate, respectively with ethyl acetate, extracting n-butyl alcohol obtains ethyl acetate, n-butanol fraction, acetic acid second
Silica gel is adsorbed in after the dissolving of ester moiety 76g acetone equal solvent, silica gel column chromatography (600g, 200-300 mesh) after drying at room temperature, with
Absolute dichloromethane, dichloromethane：Methyl alcohol (200:1、100:1、50:1、20:1 and 10:1) gradient elution obtains six cut (A-
F).D cuts (8.5g) are through MCI post (methyl alcohol：Water=85:15), go out most pigment, then water-bath is concentrated under reduced pressure, then passes through
Silica gel column chromatography (is first eluted using absolute dichloromethane, dichloromethane is then used again repeatedly：Methyl alcohol=100:1 is eluted, and is obtained
To tri- cuts of D1-D3).D1 is through preparing HPLC (acetonitriles：Water=85:15) compound 1 is obtained.E cuts are through reverse C18Depigmentation
Cut (methyl alcohol afterwards：Water=60:40 elution fractions), water-bath is concentrated under reduced pressure.Through silica gel column chromatography (dichloromethane：Methyl alcohol 50:1)
The cut E5 for obtaining is through silica gel column chromatography (dichloromethane repeatedly：Methyl alcohol=50:1), then through preparing HPLC (acetonitriles：Water=70:
30) compound 2 is obtained.F cuts are through silica gel column chromatography (dichloromethane：Methyl alcohol=20:1) five cuts are obtained.F3 is passed through repeatedly again
Silica gel column chromatography (dichloromethane：Methyl alcohol=50:1), then through preparing HPLC (acetonitriles：Water=65:35) compound 3 is obtained.
(1) noval chemical compound, its nuclear magnetic data is shown in Table compound 1：
1：White solid；[α]20 D+53.2(c 0.22,MeOH)；UV(MeOH)λmax(logε)236(4.51),272
(3.19)nm；ECD(MeOH)λmax(Δε)206(-3.09),217(-2.05),231(-3.35),248(+0.48),261(-
0.60),345(+2.98)；IR(KBr)νmax3410,3280,3060,2964,2924,2097,1705,1607,1591,1509,
1404,1320,1223,1177,999,971,870,818,777,601cm-1,1H NMR(CDCl3,400MHz)and 13C NMR
(CDCl3, 100MHz) and data, it is shown in Table 1；HRESIMS m/z 309.1495[M-H]-(calcd for C20H21O3,
309.1496), absolute configuration is 2R, 3S.
The nuclear magnetic data of the compound 1 of table 1
The drug effect related experiment of the compound of the gained of 2 embodiment of embodiment 1
The present invention utilizes broth dilution method for the antibacterial activity test screen of compound 1~3, i.e., by test chemical combination
The minimum inhibitory concentration (MIC value) of thing to quantitative determine its antibacterial activity, the results are shown in Table shown in 2.Specific experiment step is as follows：
(1) prepared by MH culture mediums
Take MH (Mueller-Hinton broth, OXOID) culture medium powder a certain amount of, add a certain amount of pure water,
Mix, in 121 DEG C of autoclaving 20min, 4 DEG C save backup.
(2) prepared by pancreas peptone soybean broth
Take pancreas peptone soybean broth (Tryptic broth, OXOID) culture medium powder a certain amount of, add a certain amount of
Pure water, mixes, and in 121 DEG C of autoclaving 20min, 4 DEG C save backup.
It is prepared by (3) 0.9% physiological saline
Weigh sodium chloride a certain amount of, proportionally add a certain amount of pure water, mix, in 121 DEG C of autoclavings
20min, 4 DEG C save backup.
(4) broth dilution method Determination of Antibacterial Activity
A) preparation of positive drug and testing compound：Weigh a certain amount of positive drug (vancomycin sodium and sleeping XiLin
Sodium) and testing compound, it is general that the solution to be measured that simultaneously compound concentration is 5120 μ g/mL is dissolved from dimethyl sulfoxide.
B) test is prepared with 96 orifice plates：Aseptic 96 orifice plate and culture medium and testing compound etc. are placed in aseptic operating platform
In, wherein the 1st row add 90 μ L culture mediums, the 2nd~11 row to add 50 μ L culture mediums, used as blank, its preceding four for the 12nd row
Hole adds 100 μ LMH culture mediums；Four holes add 100 μ L bacterium solutions afterwards；Last two row is positive control；
C) addition of testing compound：The testing compound solution that will be prepared, once adds the 1st of 10 orifice plates of μ L to 96
In row, drawn in then being arranged the 1st during 50 μ L are added to the 2nd row and mixed, by that analogy, to the 11st row, by last pipette tips
50 μ L mixed liquors are abandoned；
D) preparation of bacterium solution：Picking single bacterium colony bacterial strain, the bacterium equivalent to 0.5 Maxwell than turbid standard is prepared using physiological saline
Suspension (1~2 × 108), CFU/mL with culture medium 1:1000 dilution suspensions, after adding 50 μ L to dilute bacterium solution in every hole, will
96 orifice plates are placed in 35 DEG C of ± 2 DEG C of CO2gas incubators, are incubated 24h and are observed and record result；
E) result judges：It is meaningful when ability is tested under Positive control wells and blank colony growth situation normal condition.
Using absorbance of the all-wave length ELIASA in OD600 is determined per hole, the MIC of compound is then scaled according to absorbance
Value.
F) before going out result, the equal retest of each compound three times.
Antibacterial activities (μM) of the compound 1-3 of table 2 to different strain
Be can see from table 2, minimum inhibitory concentration (MIC of the compound 1 to methicillin-resistant staphylococcus aureus
Value) it is 62.5 μM, compound 2 and 3 pairs of minimum inhibitory concentrations of staphylococcus aureus (MIC value) they are 62.5 μM.This showing
Compound 1-3 can be used to prepare the medicine for preventing and treating bacterium infection.
The above embodiments merely illustrate the technical concept and features of the present invention, its object is to allow person skilled in the art
Scholar will appreciate that present disclosure and implement according to this that it is not intended to limit the scope of the present invention, all according to the present invention
The equivalent change or modification that Spirit Essence is made, should all be included within the scope of the present invention.
Claims (6)
1. a kind of mao of plain rice tree extract is preparing the application in preventing and treating bacterium infection medicine, it is characterised in that the hair plain rice
Tree extract is the one kind in following structural formula：
2. application according to claim 1, it is characterised in that the hair plain rice tree extract is to prepare the resistance to methoxy of preventing and treating
Application in XiLin staphylococcus aureus and/or infection of staphylococcus aureus medicine.
3. application according to claim 1 and 2, it is characterised in that the hair plain rice tree extract is：
4. application according to claim 1, it is characterised in that the medicine includes pharmaceutically acceptable carrier or auxiliary
Material.
5. a kind of mao plain rice tree extract, it is characterised in that the hair plain rice tree extract is the compound of following structural formula：
6. a kind of mao of preparation method of plain rice tree extract, it is characterised in that by hair plain rice tree (Flueggea acicularis)
Complete stool, dries, and crushes, and is extracted three times with 95% ethanol room temperature, 24 hours every time, merges concentrate, respectively with ethyl acetate, just
Butanol, before immunoassay, obtains ethyl acetate portion and n-butanol fraction, then by ethyl acetate portion by silica gel column chromatography, dichloromethane
Alkane：Methyl alcohol is respectively 200 according to percentage:1、100:1、50:1、20:1 and 10:1 gradient elution obtains six cuts, and cut is again
By repeatedly isolated, the hair plain rice tree extract is the one kind in following chemical formula：
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611186026.5A CN106860435A (en) | 2016-12-20 | 2016-12-20 | A kind of mao of plain rice tree extract and preparation method thereof and preparing the application in preventing and treating bacterium infection medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611186026.5A CN106860435A (en) | 2016-12-20 | 2016-12-20 | A kind of mao of plain rice tree extract and preparation method thereof and preparing the application in preventing and treating bacterium infection medicine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106860435A true CN106860435A (en) | 2017-06-20 |
Family
ID=59164754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611186026.5A Pending CN106860435A (en) | 2016-12-20 | 2016-12-20 | A kind of mao of plain rice tree extract and preparation method thereof and preparing the application in preventing and treating bacterium infection medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106860435A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109875985A (en) * | 2018-12-29 | 2019-06-14 | 中山大学 | A kind of mao of plain rice tree extract and its preparing the application in osteosporosis resistant medicament |
-
2016
- 2016-12-20 CN CN201611186026.5A patent/CN106860435A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 吉月菊等: "白饭树枝叶的化学成分研究(II)", 《广东化工》 * |
| 陈泳等: "树脂酸及其衍生物的抗肿瘤和抗微生物活性研究新进展", 《林产化学与工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109875985A (en) * | 2018-12-29 | 2019-06-14 | 中山大学 | A kind of mao of plain rice tree extract and its preparing the application in osteosporosis resistant medicament |
| CN109875985B (en) * | 2018-12-29 | 2021-04-02 | 中山大学 | A Vaccinium bracteatum extract and its application in preparing medicine for treating osteoporosis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rocchetti et al. | Phenolic profiling and in vitro bioactivity of Moringa oleifera leaves as affected by different extraction solvents | |
| Najafi et al. | Phytochemical screening and antibacterial activity of Citrullus colocynthis (Linn.) Schrad against Staphylococcus aureus | |
| Kumar et al. | Phytochemical investication and heptoprotective evalution acacia rubica extract isonized and paracetamol indused animal toxicity | |
| Ncube et al. | Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends | |
| Oladipupo et al. | Phytochemical analysis and antimicrobial effect of Chrysophillum albidum leave extract on gastrointestinal tract pathogenic bacteria and fungi in human | |
| Wu et al. | Enhancement on antioxidant, anti-hyperglycemic and antibacterial activities of blackberry anthocyanins by processes optimization involving extraction and purification | |
| CN106860435A (en) | A kind of mao of plain rice tree extract and preparation method thereof and preparing the application in preventing and treating bacterium infection medicine | |
| Davoodi et al. | The chemical composition and antibacterial activity of a methanolic extract of Satureja khuzistanica | |
| CN107260774A (en) | The purposes of wax-cakes bait Genes For Plant Tolerance pathogenic infection | |
| CN108853068A (en) | A kind of farnesyl phenolic compound grifolin and its pharmaceutical composition and its application | |
| Lima et al. | Anti-Candida and anti-Cryptococcus evaluation of 15 non-alkaloidal compounds from Pterogyne nitens | |
| Jaradat et al. | Preliminary phytochemical screening and in-vitro evaluation of antioxidant and antimicrobial activities for Astragalus pelecinus from Palestine | |
| Njokuocha et al. | Antibacterial and phytochemical properties of crude leaf extracts of Moringa oleifera Lam., Pterocarpus santalinoides L’Herit DC and Ceiba pentandra L. on some clinical bacterial isolates in Nigeria | |
| KR100976927B1 (en) | Antibacterial activity of methyl gallate isolated from the Galla Rhois in combination with ciprofloxacin aganist salmonella | |
| CN108003099B (en) | Bisbenzyl tetrahydroisoquinoline compound and preparation method and application thereof | |
| Gao et al. | Differential metabolome landscape of Kadsura coccinea fruit tissues and potential valorization of the peel and seed tissues | |
| CN103889430A (en) | Honeysuckle extract, and pharmaceutical composition comprising the same and use of the same | |
| CN110172016B (en) | Benzyl alcohol compound, pharmaceutical composition and application thereof | |
| Ejimofor et al. | Determination of antimicrobial susceptibility pattern of bacteria isolated from herbal drugs hawked in Onitsha Anambra State Nigeria | |
| Kumar et al. | Evaluation of in vitro antibacterial property of some plants of subtropical climate against Rhodococcus equi | |
| Akuodor et al. | In vitro antimicrobial activity of leaf extract of Berlina grandiflora Hutch. and Dalz. | |
| Pitre-Ruiz et al. | In vitro antimicrobial activity of Caesalpinia coriaria (Jacq.) Willd extracts on Streptococcus pyogenes and Candida albicans | |
| CN105496962B (en) | A kind of hydrochloric acid sarafloxacin traditional Chinese medicine extraction composite lipidosome and preparation method thereof | |
| CN108926585A (en) | Application of the wintersweet genus plants extract in pharmacy | |
| CN104887677B (en) | Natural naphthoquinones dimer and its purposes in preparing antibacterials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170620 |