CN106857499A - Tea Polyphenols is used to prepare the application of pig semen preservation dilution with shitosan compatibility - Google Patents
Tea Polyphenols is used to prepare the application of pig semen preservation dilution with shitosan compatibility Download PDFInfo
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- CN106857499A CN106857499A CN201710004919.1A CN201710004919A CN106857499A CN 106857499 A CN106857499 A CN 106857499A CN 201710004919 A CN201710004919 A CN 201710004919A CN 106857499 A CN106857499 A CN 106857499A
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- shitosan
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- 210000000582 semen Anatomy 0.000 title claims abstract description 51
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 38
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 37
- 238000010790 dilution Methods 0.000 title claims abstract description 29
- 239000012895 dilution Substances 0.000 title claims abstract description 29
- 238000004321 preservation Methods 0.000 title claims abstract description 24
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 10
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 4
- 235000011164 potassium chloride Nutrition 0.000 claims description 4
- 239000001509 sodium citrate Substances 0.000 claims description 4
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 239000003963 antioxidant agent Substances 0.000 abstract description 9
- 230000003078 antioxidant effect Effects 0.000 abstract description 9
- 230000000694 effects Effects 0.000 description 31
- 241000282898 Sus scrofa Species 0.000 description 11
- 235000013350 formula milk Nutrition 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 7
- 244000269722 Thea sinensis Species 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 230000019100 sperm motility Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 230000004899 motility Effects 0.000 description 5
- 229920001661 Chitosan Polymers 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000209094 Oryza Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 235000015165 citric acid Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 235000020610 powder formula Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 235000011083 sodium citrates Nutrition 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102100026041 Acrosin Human genes 0.000 description 1
- 108090000107 Acrosin Proteins 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- -1 oxygen radical Chemical class 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 208000017443 reproductive system disease Diseases 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- USFPINLPPFWTJW-UHFFFAOYSA-N tetraphenylphosphonium Chemical compound C1=CC=CC=C1[P+](C=1C=CC=CC=1)(C=1C=CC=CC=1)C1=CC=CC=C1 USFPINLPPFWTJW-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical class [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physiology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to Tea Polyphenols and shitosan compatibility as pig semen preservation dilution application.The antioxidant that porcine semen at normal temperature of the invention is preserved; as a result of Tea Polyphenols and shitosan compatibility as antioxidant; there is the conspiracy relation of dependence between the two, the Tea Polyphenols of debita spissitudo can well protect the physiologically active of Boar spermatozoa with shitosan compatibility during porcine semen at normal temperature preservation.
Description
Technical field
Formula of liquid and its most is diluted the present invention relates to be used to preparing the Tea Polyphenols of porcine semen at normal temperature preservation and shitosan compatibility
Good compatibility concentration, can effectively improve Semen routine effect, and can effectively reduce the content of bacterium in seminal fluid, improve antioxygen in seminal fluid
Change the activity of enzyme.
Background technology
The normal temperature of pig semen preserves the effective storage in vitro time for extending sperm, and the kind that breeding boar is improved conscientiously is used
Value, reduces the generation of genital system diseases, promotes the development and application of Artificial Insemination Technology of Swine.Because normal temperature preserves dilution powder
Formula is the key for determining porcine semen at normal temperature preservation effect, and country's porcine semen at normal temperature preservation dilution powder formula still cannot be complete at present
The full monopolization for breaking foreign countries, thus the normal temperature of pig semen preserves the dilution powder for needing more efficient stable.But, dilution powder formula
In protectant species used and consumption be the core for determining preservation effect after semen dilution, and ratio with other compositions and match somebody with somebody
5 physiological status and activity that will be directly connected to sperm after preservation.
Active oxygen (ROS) is the unstable metabolite (appointing pretty tinkling of pieces of jade etc. 2013) of generation during cell aerobic metabolism.Feed
There is certain ROS systems of defense (Awda et al.2009), predominantly enzyme antioxidant system in newborn animal semen, bag in itself
Include SOD, CAT and GSH-PX.Although the activities of antioxidant enzymes between different plant species is not quite similar (Strzezek et al.2002),
But for same animal, SOD activity can reflect the level of whole system antioxidase, SOD higher, the oxidation resistances of activity
It is stronger.Although CAT and GSH-PX plays synergism during SOD removes free radical, CAT and GSH-PX activity also from
The antioxidant levels (Orzolek et al.2013) of the system are reflected in side.Therefore, in test in laboratory cell metabolism system
Antioxidant levels generally detect its activity using SOD, CAT and GSH-PX kit.H2O2It is one kind of oxygen radical, it is excessive
H2O2Cell can be caused to damage.For spermatoblast, the fertility of sperm, thus H in body can be destroyed2O2Contain
Amount can also be used as the Testing index of plasmalemmae of sperms peroxide injury.By determining the change of these indexs, reflection sperm is dilute
Release the Survival in pendular ring border.
The normal temperature of seminal fluid preserve be one under the conditions of 17 DEG C, the sperm in vitro time-to-live is extended by adding dilution
Technology.Acrosin can reach optimum activity (Pinart 2013) at 17 DEG C.Because sperm is under 17 DEG C of environment
Can carry out normal metabolic activity, produce generally require in harmful substance, thus dilution add corresponding antioxidant or
Other drugs, such as mycillin, keep the relative stability of its microenvironment.
The content of the invention
The present invention is added not using Tea Polyphenols (TPP) and shitosan (CS) as the main component of antioxidant by combining
With the Tea Polyphenols and shitosan of concentration, with the MDA contents in the motility rate of sperm, plasm membrane integrity, seminal fluid after dilution, CAT activity,
SOD activity, GSH-Px activity and the optimal compatibility concentration that bacterial content is deliberated index, screening Tea Polyphenols and shitosan, to protect
Card pig semen maintains good bioactivity during normal temperature preservation.
The invention provides Tea Polyphenols and shitosan compatibility as porcine semen at normal temperature preservation dilution application.
Present invention also offers a kind of porcine semen at normal temperature preservation dilution, the normal temperature preserve dilution comprising Tea Polyphenols with
Shitosan.
Further, porcine semen at normal temperature preservation dilution component of the invention is：Porcine semen at normal temperature described in per 1L is preserved to be used
The formula of dilution is：Tea Polyphenols 0.02g, shitosan 0.05g, glucose 38g, sodium citrate 6.5g, EDTA 1.2g, carbonic acid
Hydrogen sodium 1g, citric acid 0.25g, potassium chloride 0.6g, beta-schardinger dextrin 0.9g, remaining is ultra-pure water.
The antioxidant that porcine semen at normal temperature of the invention is preserved, as a result of Tea Polyphenols and shitosan compatibility as antioxygen
Agent, the conspiracy relation that there is dependence between the two, Tea Polyphenols and the shitosan compatibility of debita spissitudo can be normal in pig semen
Temperature protects the physiologically active of Boar spermatozoa well during preserving.
Brief description of the drawings
Fig. 1 preserves the active influences of pig semen SOD for (17 DEG C) for the tea polyphenol-chitosan compatibility of various concentrations to normal temperature；
Fig. 2 preserves the active influences of pig semen CAT for (17 DEG C) for the tea polyphenol-chitosan compatibility of various concentrations to normal temperature；
Fig. 3 preserves the active shadows of pig semen GSH-PX for (17 DEG C) for the tea polyphenol-chitosan compatibility of various concentrations to normal temperature
Ring；
Fig. 4 is the tea polyphenol-chitosan compatibility of various concentrations to (17 DEG C) influences of preservation pig semen bacterial content of normal temperature.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.
According to technical scheme, the present embodiment provides a kind of Tea Polyphenols preserved for porcine semen at normal temperature and shell gathers
Sugared compatibility dilutes formula of liquid.Wherein：
The formula of dilution is：Tea Polyphenols 0.02g/L, shitosan 0.05g/L, glucose 38g/L, sodium citrate 6.5g/
L, EDTA 1.2g/L, sodium acid carbonate 1g/L, citric acid 0.25g/L, potassium chloride 0.6g/L, beta-schardinger dextrin 0.9g/L (table 1).
The Tea Polyphenols of table 1 dilutes formula of liquid with shitosan compatibility
Experiment detailed process is as follows：
Dilution formula of liquid according to table 1, weighs 38g glucose, 6.5g sodium citrates, 1.2g EDTA, 1g sodium acid carbonates,
0.25g citric acids, 0.6g potassium chloride, 0.9g beta-schardinger dextrins, weigh respectively 0g, 0.01g, 0.02g, 0.04g Tea Polyphenols and 0g,
0.05g, 0.1g shitosan.Tea Polyphenols is added with shitosan according to the compatibility program of table 2, fixed after being dissolved in 250mL sterilizing beakers
1000mL volumetric flasks are dissolved in, 0.22 μm of bacteriological filtration membrane filtration solution is coordinated with sterilizing filter in aseptic operating platform after 1h, obtained final product
To the mass concentration of Tea Polyphenols and shitosan, the dilution for preparing is stand-by.
The Tea Polyphenols of table 2 and shitosan compatibility dilution formula of liquid
Pig semen comes from 20 monthly age Duroc herd boars used by this experiment.After the seminal fluid filtering that will be collected using hand grip
Incubator is put into, laboratory is taken back rapidly.Choose color and luster and smell is normal, seminal fluid of the sperm motility rate more than 85% is stand-by.
2mL seminal fluid is taken in aseptic 10mL centrifuge tubes, then is slowly added dilution to the mouth of pipe, 4 layers of towel wrap up centrifuge tube,
6d is preserved in being put into 17 DEG C of insulating boxs when seminal fluid temperature and room temperature are close, seminal fluid is slowly stirred 1 time every 12h, every 24h inspections
Look into sperm motility rate (being effective holding time with the number of days that sperm motility rate is 60%), plasm membrane integrity；Respectively Semen routine 0,
2nd, 4, bacterial content during 6d in detection seminal fluid.Each treatment is repeated 3 times.
Sperm motility rate, plasm membrane integrity, SOD activity, CAT activity, GSH-Px activity and bacterial content measurement result with
The diagram form of table sum is represented.
Boar spermatozoa motility rate (%) after the Tea Polyphenols and shitosan compatibility of the various dose of table 3
Boar spermatozoa plasm membrane integrity (%) after the Tea Polyphenols and shitosan compatibility of the various dose of table 4
As can be seen from Table 3, except T3C2And T3C0Outward, other compatibility treatment group sperm effective holding times can reach 6d,
And sperm motility rate and control group significant difference (P ＜ 0.05) when preserving 6d；T2C1Sperm motility rate is most during whole preservation for group
Height, and it is significantly higher than individually addition Tea Polyphenols or shitosan treatment group；Sperm motility rate is 67.40% when preserving 6d, is significantly higher than
Other each groups (P ＜ 0.05).With the increase of combined concentration, high concentration group T3C2Sperm motility rate is minimum when preserving 6d, therefore this is dense
Degree combination is unfavorable for Semen routine.
As can be seen from Table 4, plasmalemmae of sperms percentage of head rice is reduced with the holding time, and each treatment group plasma membrane is complete when normal temperature preserves 1d
Whole rate difference is not notable (P ＞ 0.05)；Since 2d is preserved, the treatment group plasmalemmae of sperms of joint addition Tea Polyphenols and shitosan
Percentage of head rice is significantly higher than the treatment group of individually addition Tea Polyphenols；Normal temperature preserves 2~5d, except T3C2Outer remaining treatment group plasma membrane is complete
Rate is significantly higher than control group (P ＜ 0.05), but most difference is not notable (P ＞ 0.05) between treatment group；After preserving 6d, T2C1Group matter
Film percentage of head rice highest, is significantly higher than exclusive use Tea Polyphenols treatment group (P ＜ 0.05), but poor with shitosan treatment group is used alone
Different not notable (P ＞ 0.05), T3C2Treatment group plasm membrane integrity is minimum, substantially less than other treatment groups (P ＜ 0.05).
As seen from Figure 1, each treatment group SOD activity is reduced with the holding time, preserves T after 2d1C2、T2C1、T2C2Group is equal
Higher than the T of individually addition Tea Polyphenols and shitosan0C2And T3C0Group, but difference is not notable (P ＞ 0.05)；Preserve T after 4d2C1Group according to
Highest SOD activity is so kept, but difference is not notable (P ＞ 0.05)；Two groups of SOD activity highest is T after preserving 6d2C1And T2C2,
And it is significantly higher than other treatment groups (P ＜ 0.05), wherein T2C1Higher than T2C2, but two group differences are not notable (P ＞ 0.05).
As shown in Figure 2, T2C1Treatment group is favorably improved CAT activity during porcine semen at normal temperature is preserved.By 3 measurement data
Show, T2C1The CAT activity for the treatment of group is significantly higher than the T of individually addition Tea Polyphenols and shitosan0C2And T3C0Group (P ＜ 0.05),
Slightly above T1C2、T2C2Two groups, but difference is not notable (P ＞ 0.05).Tea Polyphenols group is individually added, CAT activity is higher than individually addition
Shitosan group, but difference is not notable (P ＞ 0.05).In general, joint addition Tea Polyphenols is better than list with the effect of shitosan
Only additive effect.T2C1To improving CAT active effects preferably, optium concentration is combined as 0.02g/L TPP+0.05g/L CS.
From the figure 3, it may be seen that GSH-PX activity is changed greatly with the holding time, and during preservation, T3C0Group effect is better than T0C2
And control group, but difference is not notable (P ＞ 0.05).T2C1Treatment group can improve GSH-PX activity, and effect is preferably, but and other
Group difference is not notable (P ＞ 0.05).
As shown in Figure 4, bacterial content changes with the Semen routine time in seminal fluid, and 0~2d increases more slow, 2~4d
Growth rate is most fast, and 4~6d growth rate are again relative to be slowed down, and during wherein 0~4d of Semen routine, complexing agent antibacterial effect is better than
Individually two kinds of materials of addition, but difference are not notable (P ＞ 0.05).After Semen routine 6d, control group seminal fluid bacterial content is far above
Other treatment groups (P ＜ 0.05), compatibility group T1C1Bacterial content is higher than individually addition Tea Polyphenols and shitosan treatment group (P ＜
0.05), T2C1Two kinds of material treatment groups (P ＜ 0.05) are substantially less than individually added, and with the increase of combined concentration, seminal fluid
Middle bacterial content is gradually decreased, high concentration combined treatment group T3C2Bacterial content is minimum.
Compared with existing porcine semen at normal temperature preservation dilution, the dilution that this research institute uses is simultaneously more containing tea
Phenol and shitosan, this is the innovative point of this research.The result of study shows that joint adds appropriate Tea Polyphenols and shitosan (example
As being 0.02g/L Tea Polyphenols and 0.05g/L shitosans in above-mentioned embodiment) conventional parameter (essence of Boar spermatozoa can be significantly improved
Sub- motility rate, plasm membrane integrity), effective holding time is up to 6d.In addition, present invention research finds that the tea of suitable concn is more
Phenol can not only improve antioxidase (SOD, the CAT, GSH-PX) activity of sperm with shitosan compatibility, so as to protect sperm to exempt from
Oxidative damage, but also growth rate of the bacterium during porcine semen at normal temperature preservation can be reduced, it is Boar spermatozoa in normal temperature
Possess good microenvironment during preservation and provide guarantee.
It should be appreciated that for those of ordinary skills, can according to the above description be subject to modifications and variations, and
All these modifications and variations should all belong to the protection domain of appended claims of the present invention, and these modifications and variations are caused
T2C1Group is that 0.02g/L Tea Polyphenols can not be pre- relative to existing technical merit with the preservation effect under 0.05g/L shitosan formulas
Material.
Claims (3)
1. Tea Polyphenols and shitosan compatibility as porcine semen at normal temperature preservation dilution application.
2. a kind of porcine semen at normal temperature preservation dilution, it is characterised in that the normal temperature preserves dilution and gathers with shell comprising Tea Polyphenols
Sugar.
3. porcine semen at normal temperature preservation dilution as claimed in claim 2, it is characterised in that the normal temperature preserves dilution component
For：
The formula of porcine semen at normal temperature preservation dilution is described in per 1L：Tea Polyphenols 0.02g, shitosan 0.05g, glucose 38g,
Sodium citrate 6.5g, EDTA 1.2g, sodium acid carbonate 1g, citric acid 0.25g, potassium chloride 0.6g, beta-schardinger dextrin 0.9g, remaining is
Ultra-pure water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710004919.1A CN106857499B (en) | 2017-01-04 | 2017-01-04 | Application of tea polyphenol and chitosan in preparation of diluent for preserving boar semen |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710004919.1A CN106857499B (en) | 2017-01-04 | 2017-01-04 | Application of tea polyphenol and chitosan in preparation of diluent for preserving boar semen |
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| Publication Number | Publication Date |
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| CN106857499A true CN106857499A (en) | 2017-06-20 |
| CN106857499B CN106857499B (en) | 2020-04-21 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107637589A (en) * | 2017-11-21 | 2018-01-30 | 广东中农联生物制药有限公司 | Diluent of Pig's Spermatic Fluid and its production method |
| CN107873697A (en) * | 2017-11-21 | 2018-04-06 | 广东中农联生物制药有限公司 | One kind partner's formulation Diluent of Pig's Spermatic Fluid |
| CN113317312A (en) * | 2021-06-01 | 2021-08-31 | 福建农林大学 | Diluent preparation capable of prolonging normal-temperature survival period of boar semen |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817158A (en) * | 2006-03-14 | 2006-08-16 | 中国农业科学院茶叶研究所 | Green-tea biological antistaling agent at normal temperature and use thereof |
| CN102106588A (en) * | 2009-12-25 | 2011-06-29 | 南通远大生物科技发展有限公司 | Preservative for water-swollen squid and using method thereof |
| CN104996543A (en) * | 2015-07-31 | 2015-10-28 | 广东海洋大学 | Improved biological preservation agent for aquatic products, as well as preparation method and application thereof |
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2017
- 2017-01-04 CN CN201710004919.1A patent/CN106857499B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817158A (en) * | 2006-03-14 | 2006-08-16 | 中国农业科学院茶叶研究所 | Green-tea biological antistaling agent at normal temperature and use thereof |
| CN102106588A (en) * | 2009-12-25 | 2011-06-29 | 南通远大生物科技发展有限公司 | Preservative for water-swollen squid and using method thereof |
| CN104996543A (en) * | 2015-07-31 | 2015-10-28 | 广东海洋大学 | Improved biological preservation agent for aquatic products, as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
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| 彭夏云 等: "茶多酚对猪精液低温保存的影响", 《江苏农业科学》 * |
| 胡建宏 等: "稀释液成分对猪精液常温保存效果的影响", 《黑龙江畜牧兽医》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107637589A (en) * | 2017-11-21 | 2018-01-30 | 广东中农联生物制药有限公司 | Diluent of Pig's Spermatic Fluid and its production method |
| CN107873697A (en) * | 2017-11-21 | 2018-04-06 | 广东中农联生物制药有限公司 | One kind partner's formulation Diluent of Pig's Spermatic Fluid |
| CN113317312A (en) * | 2021-06-01 | 2021-08-31 | 福建农林大学 | Diluent preparation capable of prolonging normal-temperature survival period of boar semen |
| CN113317312B (en) * | 2021-06-01 | 2022-07-12 | 福建农林大学 | Diluent preparation capable of prolonging normal-temperature survival period of boar semen |
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| Publication number | Publication date |
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| CN106857499B (en) | 2020-04-21 |
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