CN106854643A - The plant extract of lifting grain wire body activity - Google Patents
The plant extract of lifting grain wire body activity Download PDFInfo
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- CN106854643A CN106854643A CN201510897080.XA CN201510897080A CN106854643A CN 106854643 A CN106854643 A CN 106854643A CN 201510897080 A CN201510897080 A CN 201510897080A CN 106854643 A CN106854643 A CN 106854643A
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- wire body
- grain wire
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
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Abstract
The present invention is on a kind of plant extract for lifting grain wire body activity, wherein the plant extract includes plant polyphenol.Plant extract of the present invention can effectively lift the ability that a wire body manufactures ATP.
Description
Technical field
The present invention is that, on a kind of plant extract for lifting grain wire body activity, particularly one kind contains
The plant extract of plant polyphenol.
Background technology
Grain wire body (mitochondria, mitochondrion), it is most of true to be that one kind is present in
In nucleus and by the coated born of the same parents' device of two membranes, diameter is in 0.5 to 10 microns.
Most of eukaryotics are more or less owned by a wire body, but each of which possesses
Grain wire body is all different in the aspects such as size, quantity and outward appearance.This born of the same parents' device
Possess the inhereditary material and genetic system of itself, but because its Genome Size is limited,
So grain wire body is a kind of semi-autonomous born of the same parents' device.Grain wire body be it is intracellular aoxidized,
Phosphorylation and the main place of synthesis atriphos (ATP), are the activity of cell
There is provided chemical energy, so there is " power station of cell " (the powerhouse of
The cell) title.In addition to energy supply, grain wire body also participates in such as cell differentiation, thin
The process such as born of the same parents' information transmission and Apoptosis, and possess regulating cell growth and cell
The ability in cycle.
Normal cell contains several to over thousands of grain wire bodies, and studying confirms, old man's cell
Interior grain wire body content is significantly reduced.Additionally, mistake of the grain wire body in conversion energy
Cheng Zhongxu employs electron transmission.So if not capturing electronics correctly, escape
Electrons and oxygen molecule be combined into superoxide radical, it is easy to destroy base and make
Granulating wire body DNA mutation, and then accumulation some diseases.
Because grain wire body is the energy plants of cell, and there is close pass with many diseases
System, therefore how to protect a wire body not destroyed, and it is maintained normal function,
It is considered as very important R＆D direction on current clinical medicine.
The content of the invention
In view of this, the present invention provides a kind of plant extract is used to prepare the use of lifting grain wire body activity
On the way, wherein the plant extract is plant polyphenol.
In one embodiment of this invention, wherein the lifting grain wire body activity refers to that can effectively lift a line
The basal energy of body, lifting grain wire body manufacture the ability of ATP, the end-point energy of lifting grain wire body, carry
Rise prestore energy and the lifting unit grain wire body energy of grain wire body.
In an embodiment of the present invention, wherein the lifting grain wire body activity refers to reduce grain wire body free radical
Leakage.
In an embodiment of the present invention, wherein the plant polyphenol is green tea polyphenols.
In an embodiment of the present invention, wherein the concentration of the green tea polyphenols is 25~125 μ g/ml.
In the preferred embodiment, wherein the concentration of the green tea polyphenols is 50 μ g/ml.
The present invention separately provide a kind of plant extract for prepare the active purposes of protection grain wire body, wherein institute
Plant extract is stated for plant polyphenol.
In an embodiment of the present invention, wherein the plant polyphenol is green tea polyphenols.
In an embodiment of the present invention, wherein the concentration of the green tea polyphenols is 25~125 μ g/ml.
It is used to prepare the purposes for increasing stem cell proliferation the present invention further provides a kind of plant extract, its
Described in plant extract be plant polyphenol.
In the preferred embodiment, wherein the plant polyphenol includes apple polyphenol, green tea polyphenols
Or Red Wine Polyphenols.
Brief description of the drawings
Basal energy influence of the green tea polyphenols of Fig. 1 display various concentrations on grain wire body.
Free radical leakage contribution of the green tea polyphenols of Fig. 2 display various concentrations to grain wire body.
The green tea polyphenols of Fig. 3 display various concentrations manufactures the influence of ATP abilities to grain wire body.
End-point energy influence of the green tea polyphenols of Fig. 4 display various concentrations on grain wire body.
Prestore energy affect of the green tea polyphenols of Fig. 5 display various concentrations to grain wire body.
The energy affect that the green tea polyphenols of Fig. 6 display various concentrations is produced to the unit grain wire body of grain wire body.
Fig. 7 A are the influence result figure of the Red Wine Polyphenols to fatty mesenchymal stem cell hyperplasia of various concentrations；
Fig. 7 B are the influence result figure of the green tea polyphenols to fatty mesenchymal stem cell hyperplasia of various concentrations；
Fig. 7 C are the influence result figure of the apple polyphenol to fatty mesenchymal stem cell hyperplasia of various concentrations；
Specific embodiment
Heretofore described plant polyphenol refers to green tea polyphenols, apple polyphenol or black tea polyphenols etc..
Heretofore described " Red Wine Polyphenols " refers to the polyphenolic substance in red wine, comprising various chemicals
Matter, such as phenolic acid class, flavonols (dihydroflavonols), resveratrol, anthocyanidin, tannin.
Heretofore described " green tea polyphenols " refers to the presence of the polyphenolic substance in tealeaves, including but is not limited
In：Epicatechin (epicatechin, EC), epigallocatechin (epigallocatechin, EGC),
L-Epicatechin gallate (epicatechin gallate, ECG), EGCG
Ester (epigallocatechin gallate, EGCg) etc..
Heretofore described " apple polyphenol " is the common name of contained polyatomic phenol material in apple, is included
Chlorogenic acid, catechin, epicatechin, apple condensed tannin, phloridzin, phloretin, anthocyanidin etc.,
Wherein apple condensed tannin accounts for the half of polyhydric phenols total content.
Plant extract of the invention can give individuality and use by the mode such as oral, nasal inhalation, percutaneous.
Additionally, the dosage of plant extract of the present invention can do appropriate adjustment needed for according to Different Individual.
Embodiment
1. prepared by cell
Using the cell culture method of two-part, smooth cell monolayer is produced.
(1) be suspended in the cell needed for each hole (well) in culture dish among 100 μ l culture mediums by, and one
As for the cell that uses required for each well it is rough between 20,000 to 60,000 cells.
(2) cell suspending liquid that will be prepared is added among well, is advised micro suction when adding culture medium
The top for managing (pipetor) is butted on the hole wall of small hole at bottom part, slowly adds in hole reaching most culture medium
Good effect, it is to be noted that culture medium may only be added in " background correction Well ", it is not necessary to plus
Enter any cell.
(3) be placed in cell culture in CO2gas incubator and cultivate by, until cell has been attached at training
Support the bottom of disk.In general be easier to attach cell take around one to two hours, some compared with
The cell for being not easy to attach may need or so six hours.
(4) after treats cell attachment, appropriate culture medium is added in each hole, it is however generally that with every
Advise adding the culture medium of about 150 μ l or so for night culture, when noting adding culture medium
Invitation card adds to avoid disturbing the cell for being attached at bottom slowly in container wall.
(5) will add the cell culture after culture medium to be placed in CO2gas incubator culture overnight.
2. cell culture medium
Among the process of experiment, it may be desirable to prepare two distinct types of culture medium, the first is to allow cell
The culture medium of growth, second culture medium used when being analysis.
Growth culture medium is identical with the culture medium for being generally used for cell culture, and the culture used when analyzing
Base can then remove the molecule with pH buffer capacities among general culture medium, to it is observed that pH
The change of value.In addition to pH is buffered, also should be noted that contained nutrient molecule among culture medium is
The no demand for suiting experiment.Therefore removing is needed to include 4- HEPESs in principle
(HEPES, 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid), sodium acid carbonate
(Sodium bicarbonate) and all kinds of serum, BSA equimoleculars, after the nutrient needed for adding,
Adjust pH value is certain to pH 7.4.It is furthermore noted that be all kinds of nutrient sheets added in experiment
Body also has the ability of buffered pH value, and its accurate hydrogen ion yield (PPR, proton is calculated if desired
Production rate), then need to calculate its cushion effect.
3. experiment flow
In the culture dish of 96 porose discs, 8000 fatty mesenchymal stem cells and culture medium is added to carry out per hole
Culture.After culture 24 hours, culture medium is replaced as the culture medium containing plant extract of the invention,
It is further cultured for 24 hours, then, culture medium is replaced as the H of 200mM2O2Treatment 30 minutes, uses
To destroy the shape design etc. of grain wire body, finally grain wire body is detected using hippocampus bio-energy analyzer
Basal energy, free radical leakage situation, the manufacture ability of ATP, end-point energy, prestore energy and list
The energy that position grain wire body is produced, wherein not adding plant extract of the present invention and without H2O2What is processed is thin
Born of the same parents as control group, and through H2O2Process but the cell without addition plant extract of the present invention is control
Group.
4. test of the plant extract for the basal energy of lifting grain wire body
With reference to Fig. 1, learnt by experimental result, relative to control group, through H2O2The cell for the treatment of is (i.e. right
According to group), its wire body basal energy is decreased obviously, and in experimental group, addition various concentrations (including
1.25 μ g/ml, 25 μ g/ml and 50 μ g/ml) green tea polyphenols, equal efficiency lifts intracellular grain wire body
Basal energy, and the experimental group of 50 μ g/ml green tea polyphenolses is added, compared with control group, more improve
1.54 times of basal energies of Mitochondria.
5. plant extract is for test that intracellular free radicals are leaked
With reference to Fig. 2, learnt by experimental result, relative to control group, through H2O2The cell for the treatment of is (i.e. right
According to group), the situation of its wire body free radical leakage substantially increases, and in experimental group, adds 50 μ g/ml
The experimental group of green tea polyphenols can effectively reduce a wire body free radical leakage situation, and compared with control group,
More reduce the situation of 39% free radical leakage.
6. plant extract manufactures the test of ATP abilities for lifting grain wire body
With reference to Fig. 3, learnt by experimental result, relative to control group, through H2O2The cell for the treatment of is (i.e. right
According to group), its wire body produces the ability of ATP to be decreased obviously, and in experimental group, adds various concentrations
The green tea polyphenols of (including 1.25 μ g/ml, 25 μ g/ml and 50 μ g/ml), can effectively lift a wire body system
Make the ability of ATP, and add the experimental group of 50 μ g/ml green tea polyphenolses, compared with control group, more carry
The ability of 1.8 times of manufacture ATP is risen.
7. test of the plant extract for the end-point energy of lifting grain wire body
With reference to Fig. 4, learnt by experimental result, relative to control group, through H2O2The cell for the treatment of is (i.e. right
According to group), the end-point energy of its wire body is decreased obviously, and in experimental group, addition various concentrations (including
1.25 μ g/ml, 25 μ g/ml and 50 μ g/ml) green tea polyphenols, can effectively lift the limit of a wire body
Energy, and the experimental group of 50 μ g/ml green tea polyphenolses is added, compared with control group, more improve 1.75
The end-point energy of grain wire body again.
8. the test of prestore energy of the plant extract for lifting grain wire body for meeting the pressure
With reference to Fig. 5, learnt by experimental result, relative to control group, through H2O2The cell for the treatment of is (i.e. right
According to group), the energy that prestores of its wire body is decreased obviously, and in experimental group, addition various concentrations (including
1.25 μ g/ml, 25 μ g/ml and 50 μ g/ml) green tea polyphenols, can effectively lift prestoring for a wire body
Energy, and the experimental group of 50 μ g/ml green tea polyphenolses is added, compared with control group, more improve 7.7 times
The energy that prestores.
9. test of the plant extract for lifting unit grain wire body energy
With reference to Fig. 6, learnt by experimental result, relative to control group, through H2O2The cell for the treatment of is (i.e. right
According to group), the unit grain wire body energy of its wire body is significantly reduced, and in experimental group, is added different dense
The green tea polyphenols of (including 1.25 μ g/ml, 25 μ g/ml and 50 μ g/ml) is spent, a wire body can be effectively lifted
Unit grain wire body energy, and add 50 μ g/ml green tea polyphenolses experimental group, compared with control group, more
Improve 1.2 times of unit grain wire body energy.
10. influence of the plant extract to fatty mesenchymal stem cell hyperplasia
With reference to Fig. 7 A-C, compared with the culture medium for being not added with Polyphenols, with the Red Wine Polyphenols (figure of various concentrations
7A), after green tea polyphenols (Fig. 7 B) or apple polyphenol (Fig. 7 C) are processed, fatty mesenchymal stem cell can all be promoted
Hyperplasia.
Those skilled in the art, by can easily by disclosed herein content in recognize spy of the invention
Levy, and under without departing from spirit and scope of the invention, when can carry out herein various changes, substitution with
And amendment, various conditions and purposes can be adapted to.Therefore, other embodiment also falls within power of the present invention
Within the scope of sharp claim is claimed.
Claims (9)
1. a kind of plant extract is used to prepare the purposes of lifting grain wire body activity, it is characterized in that, the plant
Extract is plant polyphenol.
2. purposes as claimed in claim 1, it is characterized in that, the lifting grain wire body activity refers to can be effective
Lift basal energy, ability, the pole of lifting grain wire body of lifting grain wire body manufacture ATP of grain wire body
Prestore energy or the lifting unit grain wire body energy of limit energy, lifting grain wire body.
3. purposes as claimed in claim 1, it is characterized in that, the lifting grain wire body activity refers to reduction grain
The leakage of wire body free radical.
4. purposes as claimed in claim 1, it is characterized in that, the plant polyphenol is green tea polyphenols.
5. a kind of plant extract is used to prepare the purposes of protection grain wire body activity, it is characterized in that, the plant
Extract is plant polyphenol.
6. purposes as claimed in claim 5, it is characterized in that, the plant extract is green tea polyphenols.
7. purposes as claimed in claim 5, it is characterized in that, the concentration of the green tea polyphenols is
25~125 μ g/ml.
8. a kind of plant extract is used to prepare the purposes for increasing stem cell proliferation, it is characterized in that, the plant
Extract is plant polyphenol.
9. purposes as claimed in claim 8, it is characterized in that, the plant polyphenol includes apple polyphenol, green
Tea Polyphenols or Red Wine Polyphenols.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510897080.XA CN106854643B (en) | 2015-12-08 | 2015-12-08 | Use of plant extracts for increasing mitochondrial activity |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510897080.XA CN106854643B (en) | 2015-12-08 | 2015-12-08 | Use of plant extracts for increasing mitochondrial activity |
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| CN106854643B CN106854643B (en) | 2021-01-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115804739A (en) * | 2021-09-15 | 2023-03-17 | 百岳特生物技术（上海）有限公司 | Fermented product of autumn plum, and its preparation method and application |
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