CN106853964A - A kind of preparation method of nano-luminescent material - Google Patents
A kind of preparation method of nano-luminescent material Download PDFInfo
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- CN106853964A CN106853964A CN201510900164.4A CN201510900164A CN106853964A CN 106853964 A CN106853964 A CN 106853964A CN 201510900164 A CN201510900164 A CN 201510900164A CN 106853964 A CN106853964 A CN 106853964A
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Abstract
The invention discloses a kind of preparation method of nano-luminescent material, its step is：After using bovine serum albumin(BSA) and glutaraldehyde cross-linking, polymer is formed, burnt through pyrocarbon in the autoclave of poly- (tetrafluoroethene), then can obtain carbon point, i.e. target nano-luminescent material by simple process again.The inventive method preparation process is simple, and greatly improves the fluorescence quantum yield of carbon point, and low cost can be completed, it is easy to promote in general chemical laboratory.The carbon point of preparation has nontoxic property, has begun to be applied to the fields such as bio-imaging, pharmaceutical carrier instead of bio-toxicity larger quantum point, and is expected to be realized in disease detection the fluorescent nano material of application.
Description
Technical field
The invention belongs to chemistry and nanometer material science field, and in particular to a kind of nano-luminescent material is high-fluorescence quantum yield carbon
The preparation method of point.
Background technology
Fluorescent carbon point is one of most popular carbon nanomaterial after fullerene, CNT and Graphene.In recent years, fluorescence
Carbon-based material increasingly causes concern.Carbon point has excellent optical property and small size property, absorptivity high, chemistry
Stability, hypotoxicity, good biocompatibility, it is easy to accomplish surface-functionalized, in biochemical sensitive, imaging analysis, environment
The fields such as detection, photocatalysis technology and pharmaceutical carrier have good application potential.But, current prepared carbon point fluorescence volume
Sub- low yield, majority is no more than 1.2%, and conventionally employed nitric acid circumfluence method causes that prepared carbon point is aoxidized by strong acid,
Change the property of carbon point, high cost, dressing agent are difficult to remove, and are not easy to promote.Therefore, in order to further widen carbon point in life
The application in substance markers field, explores new synthetic route, and economic and environment-friendly synthetic method prepares the carbon point of high-fluorescence quantum yield
It is highly desirable.
The content of the invention
It is to overcome above-mentioned the deficiencies in the prior art that the purpose of the present invention is, there is provided a kind of nanometer of high-fluorescence quantum yield carbon point is glimmering
The preparation method of luminescent material.The fluorescence quantum yield that can make carbon point by the method brings up to 11.4%, with important utilization
Value.
To achieve these goals, the technical solution adopted by the present invention is as carbon source, using poly- with bovine serum albumin(BSA) (BSA)
The autoclave of (tetrafluoroethene) method that charcoal burns at high temperature, specifically includes following steps：
(1) various sizes of BSA nano particles are prepared：Weigh appropriate BSA grain dissolutions in 1mL ultra-pure waters, so
After be added dropwise over ethanol, 5min is reacted in lucifuge, stirring, adds 8% glutaraldehyde, is stirred vigorously reaction 18h-24h.
(2) bovine serum albumin(BSA) obtained above (BSA) nano-particle is taken into 300 μ L and dilutes ten times, then by solution
It is transferred in the autoclave (15mL) of poly- (tetrafluoroethene), and heats different time at different temperatures, after the completion of reaction,
Temperature of reactor naturally cools to room temperature, the solid solution 40HZ ultrasound 30min that will be obtained then ultrafiltration centrifugation (5000rpm,
10min), lower floor's solid is discarded, then with filtration film process, (3500rpm, 10min) finally is centrifuged with 100KDa super filter tubes
Supernatant is obtained, is concentrated under reduced pressure and is removed solvent, you can obtain target nano-luminescent material.
Advantages of the present invention and innovative point：
This method is simple to operate, low cost, environmental protection, easy to spread, and there is the carbon point for being obtained fluorescent quantum high to produce
Rate, up to 11.4%.In addition, the carbon point is used as the reagent of biology sensor, to Fe3+Selectivity is high, and the response time is fast,
Strong interference immunity, while the features such as there are excellent photostability, unique luminescent properties, can bio-imaging, nano material,
The numerous areas such as environmental monitoring are widely studied and show huge potentiality.
Specific embodiment
Technical scheme is described further below in conjunction with specific embodiment.
Embodiment 1
(1) precise 20mgBSA grain dissolutions are added dropwise over 2mL ethanol in 1mL ultra-pure waters, then, lucifuge,
Stirring, reacts 5min, adds the μ L of 8% glutaraldehyde 25, is stirred vigorously reaction 18h.
(2) BSA nano-particles obtained above are taken into 300 μ L and dilutes ten times, then transfer the solution into poly- (tetrafluoro second
Alkene) autoclave (15mL) in, and 200 DEG C heat 4h, after the completion of reaction, temperature of reactor naturally cools to room temperature,
Solid solution ultrafiltration centrifugation (5000rpm, 10min) that will be obtained, discards lower floor's solid, then with film process is filtered, finally
(3500rpm, 10min) is centrifuged with 100KDa super filter tubes and obtains supernatant, be concentrated under reduced pressure and remove solvent, you can obtain target
Nano-luminescent material, its fluorescence quantum yield is 9.2%.
Embodiment 2
(1) precise 35mgBSA grain dissolutions are added dropwise over 2mL ethanol in 1mL ultra-pure waters, then, lucifuge,
Stirring, reacts 5min, adds the μ L of 8% glutaraldehyde 25, is stirred vigorously reaction 18h.
(2) BSA nano-particles obtained above are taken into 300 μ L and dilutes ten times, then transfer the solution into poly- (tetrafluoro second
Alkene) autoclave (15mL) in, and 240 DEG C heat 4h, after the completion of reaction, temperature of reactor naturally cools to room temperature,
Solid solution ultrafiltration centrifugation (5000rpm, 10min) that will be obtained, discards lower floor's solid, then with film process is filtered, finally
(3500rpm, 10min) is centrifuged with 100KDa super filter tubes and obtains supernatant, be concentrated under reduced pressure and remove solvent, you can obtain target
Nano-luminescent material, its fluorescence quantum yield is 11.4%.
Claims (4)
1. a kind of preparation method of nano-luminescent material, it is characterised in that comprise the following steps：
(1) various sizes of BSA nano particles are prepared：Appropriate BSA grain dissolutions are weighed in 1mL ultra-pure waters, Ran Houjia
Enter ethanol, lucifuge, stirring is reacted 5min, adds glutaraldehyde, is stirred vigorously reaction a period of time, obtains bovine serum albumin
(BSA) nano-particle in vain；
(2) bovine serum albumin(BSA) obtained above (BSA) nano-particle is taken into 300 μ L and dilutes ten times, then shift solution
To in the autoclave (15mL) of poly- (tetrafluoroethene), and different time is heated at different temperatures, after the completion of reaction, instead
Answer device temperature to naturally cool to room temperature, the mixture 40Hz ultrasound 30min that will be obtained, then ultrafiltration centrifugation (5000rpm,
10min), lower floor's solid is discarded, then with filtration film process, (3500rpm, 10min) finally is centrifuged with 100KDa super filter tubes
Supernatant is obtained, is concentrated under reduced pressure and is removed solvent, residue is target nano-luminescent material.
2. the preparation method of nano-luminescent material according to claim 1, it is characterized in that using the charcoal firing method of environmental protection,
With bovine serum albumin(BSA) (BSA) be carbon source, using poly- (tetrafluoroethene) autoclave at high temperature charcoal burn.
3. the preparation method of nano-luminescent material according to claim 1, it is characterized in that being added dropwise over ethanol, mass fraction is
8% glutaraldehyde.
4. the preparation method of nano-luminescent material according to claim 1, it is characterized in that prepare the reaction of BSA nano particles when
Between be 18h-24h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510900164.4A CN106853964A (en) | 2015-12-08 | 2015-12-08 | A kind of preparation method of nano-luminescent material |
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| CN201510900164.4A CN106853964A (en) | 2015-12-08 | 2015-12-08 | A kind of preparation method of nano-luminescent material |
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| CN106853964A true CN106853964A (en) | 2017-06-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109111917A (en) * | 2018-07-23 | 2019-01-01 | 中国科学院合肥物质科学研究院 | A kind of crosslinking carbon quantum dot nanosphere fluorescence probe material and the preparation method and application thereof |
| CN110606479A (en) * | 2018-06-15 | 2019-12-24 | 中国药科大学 | Simple preparation method of fluorescent carbon nanoparticles |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104709894A (en) * | 2013-12-12 | 2015-06-17 | 中国科学院大连化学物理研究所 | Preparation method and applications of fluorescent carbon quantum dots |
| CN105018082A (en) * | 2015-07-10 | 2015-11-04 | 上海纳米技术及应用国家工程研究中心有限公司 | Method for preparing carbon quantum dot labeled probe for silk fibroin extracted cell development |
-
2015
- 2015-12-08 CN CN201510900164.4A patent/CN106853964A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104709894A (en) * | 2013-12-12 | 2015-06-17 | 中国科学院大连化学物理研究所 | Preparation method and applications of fluorescent carbon quantum dots |
| CN105018082A (en) * | 2015-07-10 | 2015-11-04 | 上海纳米技术及应用国家工程研究中心有限公司 | Method for preparing carbon quantum dot labeled probe for silk fibroin extracted cell development |
Non-Patent Citations (1)
| Title |
|---|
| QINGXIU YANG ET AL.: "Single Particle Dynamic Imaging and Fe3+ Sensing with Bright Carbon Dots Derived from Bovine Serum Albumin Proteins", 《SCIENTIFIC REPORTS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110606479A (en) * | 2018-06-15 | 2019-12-24 | 中国药科大学 | Simple preparation method of fluorescent carbon nanoparticles |
| CN109111917A (en) * | 2018-07-23 | 2019-01-01 | 中国科学院合肥物质科学研究院 | A kind of crosslinking carbon quantum dot nanosphere fluorescence probe material and the preparation method and application thereof |
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Application publication date: 20170616 |