CN106841454B - The chiral analysis of nicotine closes phase chromatographic tandem mass spectrography in a kind of bolete - Google Patents
The chiral analysis of nicotine closes phase chromatographic tandem mass spectrography in a kind of bolete Download PDFInfo
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Abstract
The invention discloses a kind of chiral analysis of nicotine in bolete to close phase chromatographic tandem mass spectrography.It is characterized by: by bolete sample drying, crushing and sieving, sample is after sodium hydroxide solution infiltrates, using methyl tertiary butyl ether(MTBE) and methanol solution extraction, extract liquor is after Dispersive solid phase extraction purifies, it is connected-is closed phase chromatographic tandem analytical reagent composition using two chiral columns, using S- (-)-nicotine in areas of peak normalization method quantitative detection bolete and R- (+)-nicotine ratio.The detection method that this method provides, sample extraction purification method is simple and efficient, pollution of the sample liquid to chromatographic column can be reduced, close phase chromatographic tandem mass spectrography detection method high sensitivity, specificity is good, false positive can be excluded, analysis time is short, can satisfy the chiral analysis of nicotine in high-volume bolete sample.
Description
Technical field
The present invention relates to bolete chemical component measuring methods, and in particular to the chiral analysis of nicotine closes in a kind of bolete
Phase chromatographic tandem mass spectrography.
Background technique
Nicotine is also nicotine, is the important component of a kind of alkaloid being present in plant of Solanaceae and tobacco.Cigarette
Base molecule contains a chiral centre, i.e., 2 carbon atoms on nafoxidine ring, so there are two enantiomers for nicotine: S- (-)-
Nicotine and R- (+)-nicotine.Two enantiomers of nicotine have entirely different content, metabolic mechanism and physiological property.It therefore need to be into
The chiral analysis of nicotine in row bolete.
At present about the chiral analysis of nicotine, mainly studied using gas chromatography, such as application No. is
201610663534.1 patent " a kind of chiral analysis method of nicotine in cigarette shreds ", this method pre-treatment step is complicated,
And detection time reaches 140min, is not able to satisfy the detection of a large amount of samples, and there has been no the hands about nicotine in bolete at present
Property analysis method.It is therefore desirable to develop, a kind of high sensitivity, precision are good, are suitble to S- in high-volume accurate quantitative analysis bolete
(-)-nicotine and R- (+)-nicotine method.Chiral column-conjunction phase chromatographic tandem mass spectrum has significantly in terms of isomer separation
Advantage, therefore chiral column-conjunction phase chromatographic tandem mass spectrometric analysis method of nicotine chirality in bolete is established by many experiments,
This method is particularly suited for the chiral analysis of nicotine in a large amount of samples.
Summary of the invention
The purpose of the present invention has carried out pre-treating method research to bolete sample, has built exactly using nicotine as research object
The analysis method for having found nicotine optical isomer in series connection chiral column-conjunction phase chromatographic tandem mass spectrography detection bolete, realizes
To nicotine optical antipode S- (-)-nicotine in bolete and R- (+)-nicotine Accurate Determining.
The purpose of the present invention is achieved through the following technical solutions: by bolete sample drying, crushing and sieving, sample
Product are after sodium hydroxide solution infiltrates, and using methyl tertiary butyl ether(MTBE) and methanol solution extraction, extract liquor is through matrix dispersed solid phase
After extracting and purifying, using series connection chiral column-conjunction phase chromatographic tandem analytical reagent composition, using areas of peak normalization method quantitative detection ox
S- (-)-nicotine and R- (+)-nicotine ratio in liver bacterium.Specific step is as follows:
1. the preparation of standard solution: weighing S- (-)-nicotine standard product of about 100.0 mg, dilute constant volume with methanol solution
Into 50 mL brown volumetric flasks, it is configured to S- (-)-nicotine standard stock solution that concentration is about 2.0 mg/mL；Weigh about 50.0
The R- (+) of mg-nicotine standard product, with methanol solution dilution constant volume into 50 mL brown volumetric flasks, being configured to concentration is about 1.0
The R- (+) of mg/mL-nicotine standard stock solution；It is dilute using S- (-)-nicotine standard stock solution and R- (+)-nicotine standard stock solution
It releases, prepares S- (-)-nicotine and R- (+)-nicotinic density is respectively the mixed standard solution of 100 μ g/mL and 80 μ g/mL.
2. the mixing of bolete sample: bolete sample being placed in 40 DEG C of baking ovens, is taken out bolete after 1 hour, powder
0.45 millimeter aperture standard screen is crossed after broken.
3. the extraction of bolete sample: weighing the sample that 2 g have been crushed and have in plug centrifuge tube in 15 mL, 2.0 mL are added
5% sodium hydroxide solution, 1 min that is vortexed stand 10 min infiltration sample after mixing.Take 10 mL methyl tertiary butyl ether(MTBE)s and methanol
(90:10, V/V) solution into centrifuge tube, and on vortex mixer with 4000 rpm velocity fluctuation, 30 min, filtering supernatant
And nitrogen blows and is concentrated into 2 mL.
4. the purification of extract liquor: 1.5 mL concentrates being taken (to include 150 mg anhydrous slufuric acids in 2 mL purification centrifuge tube
Magnesium, 25 mg PSA adsorbents, 7.5 mg GCB adsorbents；It is referred to as Dispersive solid phase extraction material), on vortex mixer
2 min are vibrated with 4000 rpm, are centrifuged 1 min with 10000 rpm.Take supernatant liquid nitrogen blow be concentrated into 0.3 mL after cross 0.22 μm
The laggard conjunction phase chromatographic tandem mass spectral analysis of organic phase filter membrane.
5. chiral column-conjunction phase chromatographic tandem mass spectrography condition of connecting: analytical column 1 is Trefoil CEL1 column (column length 150
Mm, 3.0 mm of internal diameter, 2.5 μm of stationary phase partial size), analytical column 2 is Chiralcel OD-H liquid-phase chromatographic column (column length 250
Mm, 4.6 mm of internal diameter, 5 μm of stationary phase partial size)；Mobile phase A are as follows: CO2, Mobile phase B: the methanol containing 0.05% isopropylamine；Stream
Speed is 1.1 mL/min；Condition of gradient elution are as follows: 0.0 min, 97% A；0.5 min, 97% A；8.0 min, 92% A；10.0
Min, 92% A；10.2 min, 97% A；12.0 min, 97% A.40 DEG C of column temperature；Sample introduction room temperature: 10 DEG C；Sampling volume 2
μL；Back pressure: 1600 psi；ISM compensation flow path is mutually the methanol containing 0.1% formic acid, and flow velocity is 0.3 mL/min.When analysis
Between amount to 12 min.Mass Spectrometry Conditions: ion source: electrospray ionization source, scanning mode are positive ion scan, ion source temperature 300
DEG C, electron spray voltage is 5000 V, and atomization gas pressure is 40 psi；Detection mode: multiple-reaction monitoring (MRM)；MRM parameter is seen below
Table.By quantitative S- (-)-nicotine being normalized to peak area and R- (+)-nicotine accounts for the ratio of total nicotine.
The present invention provides a kind of chiral analysis of nicotine in bolete to close phase chromatographic tandem mass spectrography, has following excellent
Effect: can reduce pollution of the sample liquid to chromatographic column, close phase chromatographic tandem mass spectrography detection method high sensitivity, and specificity is good,
It can avoid false positive, analysis time is short, is able to achieve to S- (-)-nicotine and R- (+)-nicotine baseline separation, R- (+)-nicotine
Detection limit (LOD) is 0.04%, can satisfy the chiral analysis of nicotine in high-volume bolete sample.
Detailed description of the invention
Fig. 1 is the chromatogram of mixed standard solution,
Fig. 2 is the chromatogram of bolete sample.
Specific embodiment
The present invention is further described by following specific embodiments, but does not limit the present invention.
1. instrument and reagent
ACQUITY UPC2 ultra high efficiency closes chromatography (Waters, US), 6410B triplex tandem level four bars mass spectrum
Instrument (Agilent company, the U.S.), AE163 electronic balance (sensibility reciprocal: 0.0001 g, Mettler company, Switzerland), high speed disintegrator
(Wuhan Yin Cai Science and Technology Ltd.), U.S.'s ColeparmerVotex-Genie vortex oscillator, German SIGMA 3-30K- high
The desk-top frozen type centrifuge of speed.Methyl tertiary butyl ether(MTBE) (chromatographically pure), sodium hydroxide (analysis is pure), formic acid (chromatographically pure), methanol (color
Compose pure), S- (-)-nicotine standard product (CAS:54-11-5), R- (+)-nicotine standard product (CAS:25162-00-9).
2. the preparation of standard solution
S- (-)-nicotine standard product for weighing about 100.0 mg dilute constant volume to 50 mL brown volumetric flasks with methanol solution
In, it is configured to S- (-)-nicotine standard stock solution that concentration is about 2.0 mg/mL；Weigh R- (+)-nicotine mark of about 50.0 mg
Quasi- product are configured to R- (+)-cigarette that concentration is about 1.0 mg/mL with methanol solution dilution constant volume into 50 mL brown volumetric flasks
Alkali standard reserving solution；It is diluted using S- (-)-nicotine standard stock solution and R- (+)-nicotine standard stock solution, prepares S- (-)-cigarette
Alkali and R- (+)-nicotinic density are respectively the mixed standard solution of 100 μ g/mL and 80 μ g/mL.
3. the mixing of bolete sample: bolete sample being placed in 40 DEG C of baking ovens, is taken out bolete after 1 hour, powder
0.45 millimeter aperture standard screen is crossed after broken.
4. the extraction of bolete sample: weighing the sample that 2 g have been crushed and have in plug centrifuge tube in 15 mL, 2.0 mL are added
5% sodium hydroxide solution, 1 min that is vortexed stand 10 min infiltration sample after mixing.Take 10 mL methyl tertiary butyl ether(MTBE)s and methanol
(90:10, V/V) solution into centrifuge tube, and on vortex mixer with 4000 rpm velocity fluctuation, 30 min, filtering supernatant
And nitrogen blows and is concentrated into 2 mL.
5. the purification of extract liquor: 1.5 mL concentrates being taken (to include 150 mg anhydrous slufuric acids in 2 mL purification centrifuge tube
Magnesium, 25 mg PSA adsorbents, 7.5 mg GCB adsorbents), on vortex mixer with 4000 rpm vibrate 2 min, with
10000 rpm are centrifuged 1 min.Take supernatant liquid nitrogen blow be concentrated into 0.3 mL after cross the laggard conjunction phase chromatography of 0.22 μm of organic phase filter membrane
Tandem Mass Spectrometry Analysis.
6. chiral column-conjunction phase chromatographic tandem mass spectrography condition of connecting: analytical column 1 is Trefoil CEL1 column (column length 150
Mm, 3.0 mm of internal diameter, 2.5 μm of stationary phase partial size), analytical column 2 is Chiralcel OD-H liquid-phase chromatographic column (column length 250
Mm, 4.6 mm of internal diameter, 5 μm of stationary phase partial size)；Mobile phase A are as follows: CO2, Mobile phase B: the methanol containing 0.05% isopropylamine；Stream
Speed is 1.1 mL/min；Condition of gradient elution are as follows: 0.0 min, 97% A；0.5 min, 97% A；8.0 min, 92% A；10.0
Min, 92% A；10.2 min, 97% A；12.0 min, 97% A.40 DEG C of column temperature；Sample introduction room temperature: 10 DEG C；Sampling volume 2
μL；Back pressure: 1600 psi；ISM compensation flow path is mutually the methanol containing 0.1% formic acid, and flow velocity is 0.3 mL/min.When analysis
Between amount to 12 min.Mass Spectrometry Conditions: ion source: electrospray ionization source, scanning mode are positive ion scan, ion source temperature 300
DEG C, electron spray voltage is 5000 V, and atomization gas pressure is 40 psi；Detection mode: multiple-reaction monitoring (MRM)；MRM parameter is seen below
Table.By quantitative S- (-)-nicotine being normalized to peak area and R- (+)-nicotine accounts for the ratio of total nicotine.
7. R- (+)-nicotine detection limit
The chiral composition of nicotine usually accounts for the percentage of total nicotine with R- (+)-nicotine to indicate.To assess R- (+)-nicotine
Detection limit, the standard solution of R- (+)-nicotine is added in the method for the present invention in S- (-)-nicotine standard solution.The result shows that
R- (+)-nicotine response signal can be observed in S- (-)-nicotine standard solution, normalizing is carried out to quota ion peak area
After change, R- (+)-nicotine ratio is 0.04%(peak area relative to total nicotine peak area in S- (-)-nicotine standard solution),
In this, as this method R- (+)-nicotine detection limit.
8. the chiral analysis example of nicotine in bolete sample
The bolete sample handled according to above-mentioned steps is carried out to close the mass spectral analysis of phase chromatographic tandem；Using bolete nicotine
MRM ion carries out qualitative analysis, and quantitative S- (-)-nicotine is normalized to peak area and R- (+)-nicotine accounts for the ratio of total nicotine
Example.
4 bolete samples are analyzed using this method, the results are shown in Table 1.
9. the precision of analysis method
It takes same bolete sample in a few days and be in the daytime measured in parallel for 5 times, has investigated the precision of the method for the present invention, tie
Fruit is shown in Table 2 and table 3.The result shows that R- (+)-nicotine in a few days and in the daytime divide by the coefficient of variation of measurement result in bolete sample
Not Wei 2.12% and 1.71%, precision is preferable.
Above-mentioned 4 bolete samples are detected using the method in specific embodiment.Detect obtained chromatography
Figure is as depicted in figs. 1 and 2.
Claims (1)
1. the chiral analysis of nicotine closes phase chromatographic tandem mass spectrography in a kind of bolete, it is characterised in that: do bolete sample
Dry, crushing and sieving, sample is after sodium hydroxide solution infiltrates, using methyl tertiary butyl ether(MTBE) and methanol solution extraction, extraction
Liquid is analyzed using chiral column-positive liquid chromatogram UV detector, is returned using peak area after Dispersive solid phase extraction purifies
One changes S- (-)-nicotine and R- (+)-nicotine ratio in standard measure detection bolete, the specific steps are as follows:
1) preparation of standard solution: weighing S- (-)-nicotine standard product of about 100.0 mg, is arrived with methanol solution dilution constant volume
In 50 mL brown volumetric flasks, it is configured to S- (-)-nicotine standard stock solution that concentration is about 2.0 mg/mL；Weigh about 50.0 mg
R- (+)-nicotine standard product, with methanol solution dilution constant volume into 50 mL brown volumetric flasks, being configured to concentration is about 1.0
The R- (+) of mg/mL-nicotine standard stock solution；It is dilute using S- (-)-nicotine standard stock solution and R- (+)-nicotine standard stock solution
It releases preparation S- (-)-nicotine and R- (+)-nicotinic density is respectively the mixed standard solution of 100 μ g/mL and 80 μ g/mL；
2) bolete sample the mixing of bolete sample: is placed in 40oIn C baking oven, bolete is taken out after 1 hour, after crushing
Cross 0.45 millimeter aperture standard screen；
3) it the extraction of bolete sample: weighs the sample that 2 g have been crushed and has in plug centrifuge tube in 15 mL, 2.0 mL 5% are added
Sodium hydroxide solution, be vortexed 1 min mix after stand 10 min infiltration sample；Take 10 mL methyl tertiary butyl ether(MTBE)s and methanol molten
Liquid into above-mentioned tool plug centrifuge tube, and on vortex mixer with 4000 rpm velocity fluctuation, 30 min, filtering supernatant and nitrogen
It blows and is concentrated into 2 mL；The volume ratio 90:10 of methyl tertiary butyl ether(MTBE) and methanol；
4) purification of extract liquor: take 1.5 mL extraction supernatant in the purification containing Dispersive solid phase extraction material in 2 mL
In centrifuge tube, in vibrating 2 min on vortex mixer with 4000 rpm, it is centrifuged 1 min with 10000 rpm, supernatant liquid nitrogen is taken to blow
The laggard conjunction phase chromatographic tandem mass spectral analysis of 0.22 μm of organic phase filter membrane is crossed after being concentrated into 0.3 mL；The matrix dispersed solid phase
Fiber material contains: 150 mg anhydrous magnesium sulfates, 25 mg PSA adsorbents, 7.5 mg GCB adsorbents；
5) series connection chiral column-conjunction phase chromatographic tandem mass spectrography condition: analytical column 1 is Trefoil CEL1 column, 150 mm of column length
, 3.0 mm of internal diameter, 2.5 μm of stationary phase partial size, analytical column 2 be Chiralcel OD-H liquid-phase chromatographic column, 250 mm of column length,
4.6 mm of internal diameter, 5 μm of stationary phase partial size；Mobile phase A are as follows: CO2, Mobile phase B: the methanol containing 0.05% isopropylamine；Flow velocity is
1.1 mL/min；Condition of gradient elution are as follows: 0.0 min, 97% A；0.5 min, 97% A；8.0 min, 92% A；10.0 min,
92% A；10.2 min, 97% A；12.0 min, 97% A；40 DEG C of column temperature；Sample introduction room temperature: 10 DEG C；2 μ L of sampling volume；
Back pressure: 1600 psi；ISM compensation flow path is mutually the methanol containing 0.1% formic acid, and flow velocity is 0.3 mL/min；Analysis time is total
Count 12 min；
Mass Spectrometry Conditions: ion source: electrospray ionization source, scanning mode are positive ion scan, and ion source temperature is 300 DEG C, electron spray electricity
Pressure is 5000 V, and atomization gas pressure is 40 psi；Detection mode: multiple-reaction monitoring；It is quantitative by the way that peak area is normalized
S- (-)-nicotine and R- (+)-nicotine account for the ratio of total nicotine.
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| CN107505411A (en) * | 2017-08-15 | 2017-12-22 | 国家烟草质量监督检验中心 | A kind of method for closing nornicotine enantiomter content in phase chromatographic tandem mass spectrometric determination tobacco juice for electronic smoke |
| CN107478746A (en) * | 2017-08-15 | 2017-12-15 | 国家烟草质量监督检验中心 | The chiral analysis of nicotine and nornicotine close phase chromatographic tandem mass spectrography in a kind of electronic cigarette liquid |
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