CN106841179A - A kind of EUSA device - Google Patents
A kind of EUSA device Download PDFInfo
- Publication number
- CN106841179A CN106841179A CN201710144044.5A CN201710144044A CN106841179A CN 106841179 A CN106841179 A CN 106841179A CN 201710144044 A CN201710144044 A CN 201710144044A CN 106841179 A CN106841179 A CN 106841179A
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- Prior art keywords
- micropore
- elisa
- storage
- microwell plate
- lath
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
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- Health & Medical Sciences (AREA)
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- Analytical Chemistry (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
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- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
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- Food Science & Technology (AREA)
- Microbiology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
EUSA (ELISA) with microwell plate as fixing phase is widely used in clinical examination diagnosis, this method is carried out in batches in units of microwell plate, when single analysis project a large amount of samples are processed, efficiency is very high, but limited by batch mode determination when a small amount of sample, multiple projects are determined and flexibility is not good enough.The present invention relates to a kind of experimental rig for ELISA, and unlike other immunity analysis instruments：The present apparatus contains at least one set of microwell plate storage split cells, the component respectively or is applied in combination the means cutting, saw, rolling over, heating common ELISA microplates is split into single micropore, the present apparatus carries out follow-up test using the fixing phase that the single micropore that splits out reacts as ELISA, so that batch mode determination has been broken away from ELISA experiments, the discrete that realize carries out reacting independently to enter with each behavioural characteristic using common ELIAS strip is determined.
Description
Technical field
The present invention relates to the device that one kind carries out antigen or TPPA using enzyme linked immunosorbent assay (ELISA), the dress
Put the ELIAS strip and its liquid reagent of usable industry universal.Compared with common automatic enzyme micro-plate reader, it is characterised in that：
Although the present apparatus is also to be measured using common ELISA microwell plates, this equipment is before the reaction first by common microplate
The fixing phase that single micropore reacts as ELISA is disassembled into, detection reaction is determined in single " hole " with " discrete " mode operation
It is unit, the reaction of each micropore is all independently carried out.And common automatic enzyme micro-plate reader and traditional ELISA method
All it is then for unit batch is carried out with one piece of ELISA Plate (generally comprising dozens of reacting hole).
Background technology
Used as a kind of classical way for determining trace antigen, antibody, ELISA is widely used in clinical examination diagnosis, its skill
Art is ripe, widely used, and sensitivity for analysis is high, analysis specificity is good, simple to operate, is adapted to Grass-roots Hospital, prior
It is：In China, ELISA reagent industrialization levels are very high, with low cost, partial analysis project, such as Hepatitis Markers, ELISA
Reagent cost is low compared with chemiluminescence method 10-20 times.
EUSA is that one kind is immunized using polystyrene plastics cuvette (micropore) as the heterogeneous of fixing phase
Assay method is learned, general ELISA is tested used micropore and is linked to be one (lath) in units of 8-12 holes, with 4-12 lath
It is combined into a deblocking reaction plate (microwell plate), reacting hole, lath, the material of microwell plate and size all of substantially standardization；ELISA is anti-
Should be carried out with batch mode, every batch, every piece of microwell plate can simultaneously carry out the 48-96 test using identical check problem.Batch
Pattern has obvious speed advantage in the detection of single analysis project great amount of samples, but in fragmentary sample multiple detection project
It is cumbersome and time-consuming then because check problem difference need to respectively be carried out using plurality of plates multiple batch during measure.So in current each doctor
Learn laboratory not determined directly generally in the sample negligible amounts for receiving, and be to wait for collecting ability after sufficient amount of sample
The measure of a collection of concentration is carried out, wait this for some uncommon detection projects is often a couple of days in even several weeks, is given
Patient makes troubles, in some instances it may even be possible to be delayed diagnosis and treatment.And the clinical practice in modern times usually requires that laboratory to patient specimen with next
With surveying, analysis project report interior can be provided with multiple project independent assortments, 2-3 hour；So that the same day completes diagnosis and treatment, this is also base
It is difficult in the ELISA of batch mode.With the hommization increasingly of increase and the medical services of available analysis project, with change
Learn the luminous vertical method of decile to compare, the batch mode method with ELISA as representative is not only when multiple projects, fragmentary sample is dealt with
Flexibility ratio is poor, while automaticity is also very low, in today of human cost increasingly enterprise high, batch mode is just facing as ELISA
The bottleneck of bed application.
The concept of " discrete (Discrete Sample Analysis method) " is from discrete full-automatic biochemical point
Analyzer, it is characterized in that each reaction is independently to be carried out in respective reaction vessel respectively, it is advantageous that being marked per a
This check problem is all independent, therefore sample can be to do, it is not necessary to which gathering enough a collection of sample could start inspection, point
Analysis project can also be optional, and flexibility is very high, is especially suitable for middle small sample quantities, multiple analysis projects while the occasion for carrying out.
The ELISA of discrete also once ated fault the practice that loses in the 80-90 ages, was that solid phase is carried with special globule or test tube
Body, as a result precision is poor compared with chemiluminescence, and price is but similar with chemiluminescence, therefore does not obtain market accreditation.Because
The low cost of ELISA is built upon on the basis of industrialization and the huge shipment amount of conventional microplates ELISA product height, separately
The advantage that cooking stove is difficult cost is played, successful discrete ELISA is necessarily built upon on the basis of existing microwell plate ELISA
's.
But for common microwell plate, reacting hole generally even or in the way of 12 connecting plate bars is fixed on 48 holes or 96 holes by 8
On grillage, after being carried out using discrete, need to be split as lath first it is single, then in the way of similar chemiluminescent analyzer
It is measured, due to sample-adding and big and task scheduling the complexity of workload, self-reacting device is necessary.But it is existing using general
The ELISA self-reacting devices of logical microwell plate are all based on batch mode, are adapted to the automatical analysis of common microwell plate discrete pattern
There is not been reported for instrument.
The content of the invention
The invention provides a kind of self-reacting device scheme suitable for discrete pattern ELISA, the essential characteristic of the program
It is that instrument is according to measure needs by the way that common ELISA microwell plates lath to be split into single micropore as the fixing phase of reaction
The different micropore of selection, and independent enzyme-linked immunosorbent assay is carried out in each micropore, so as to realize using existing goods
The discrete that microwell plate ELISA kit is automated is determined.
This instrument include microwell plate storage with split cells, storage/conveying/reaction member, liquid reagent storage element, oneself
Dynamic liquid relief unit, micropore cleaning unit, optical density reading unit.The basic structure and working method of each unit are summarized as follows：
First, the storage of microwell plate and split cells：
This unit is storage and is split as individually reacting micro- by the lath of 8 common companies or 12 micro reaction plates even
The device in hole, is also the characteristic part of this instrument.The device includes at least one box for being used for storing lath, and at least one
It is individual for blocking micropore between coupling part cropper.The lath of micro reaction plate is stored in the box containing drier, box
Attached bag includes an opening outwards sent out for lath, and the lath sent out from opening can be truncated device and block one by one two on lath
Coupling part between micropore, is allowed to split into single reacting hole.Cropper acts on enzyme including following at least one in structure
The device of junction between each micropore of target bar, including：Blade, drift, saw blade, heater, laser cutter, in the following manner
Realize the fractionation of micropore：
1. twist off or fracture the coupling part between two micropores using external force；
2. the coupling part between external force cut-out, sawed-off or two micropores of cutting is used；
3. the coupling part between two micropores of heating power or laser blown is used；
4. both the above or three kinds of modes are applied in combination to block the coupling part between two micropores.
Independent micropore after fractionation is admitted to storage device, reaction unit or conveying device with standby.
2nd, storage/conveying/reaction member：
This unit is similar with general chemistry luminescence analyzer, the independent micropore that storage device is used for after temporary fractionation, conveying
The micropore that device is used to have split is transported to incubating device, board-washing device or optical density reading device.The realization of this unit
Mode has track and rotating disk two ways.The technology that this unit is related to belongs to known technology.
3rd, liquid reagent memory cell：
This unit is similar with general full automatic enzyme micro-plate reader, and liquid reagent is stored in room temperature or low temperature, by pipeline
System or automatic liquid relief unit are filled in micropore.The technology that this unit is related to belongs to known technology.
4th, automatic liquid relief unit：
This unit is similar with common enzyme micro-plate reader, Biochemical Analyzer and chemiluminescent analyzer, by mechanical arm with
And sample needle on the robotic arm is installed, pipe-line system pipettes liquid reagent or sample carries out ELISA reactions.What this unit was related to
Technology belongs to known technology.
5th, micropore cleaning unit：
This unit is similar with general chemistry luminescence analyzer, by being filled in micropore is reacted repeatedly and blots the side of washing lotion
Formula washes away the material not combined with solid phase antigen or antibody specificity in reacting hole.The technology that this unit is related to belongs to known technology.
6th, optical density reading unit：
This unit is similar with automatic clinical chemistry analyzer, by detecting that monochromatic light is occurred through the reaction system after colour developing
Luminous intensity change, the optical density of reaction system is changed and is converted into electric signal, and be ultimately converted to the concentration numbers of test substance
According to completion is determined.The technology that this unit is related to belongs to known technology.
Brief description of the drawings
Below in conjunction with drawings and Examples, the present invention is further described：
Fig. 1 is a basic structure for exemplary embodiments of the invention
Fig. 2 is the structural representation of the storage with split cells of microwell plate in the embodiment of the present invention
In figure：(1) storage of microwell plate and split cells, (2) reaction tray, (3) three shaft mechanical arms, (4) micropore cleaning are single
Unit, (5) optical density reading unit, (6) reagent position, (7) sample position, (8) washing lotion, (9) lath box, the plate in (10) lath boxes
Bar, (11) ratchet, (12) blades, (13) drift, (14) reaction tray, the micropore in (15) reaction tray
Specific embodiment：
In the present embodiment, as shown in figure 1, the overall structure of device includes the storage in (1) ELISA Plate hole, splits and supply dress
Put, (2) isothermal reaction disk, (3) three shaft mechanical arms, (4) micropore cleaning unit, (5) optical density reading unit and placement (6) are tried
The position of agent, (7) sample and (8) washing lotion.The fractionation of microwell plate is used using cutting off and fractureing the mode that is combined, isothermal reaction disk
Storage, conveying and incubation in micropore, three shaft mechanical arms are used for the automatic liquid relief of liquid reagent and sample.
When equipment is run, in storage and split cells (Fig. 2) lath of microwell plate are contained in lath box (9), bottom right
Angle is opened an outlet and is connected with cropper, and cropper critical piece is ratchet (11), blade (12) and drift (13).In ratchet (11)
Promotion lower plate barrel in lath (10) sent out and clamped by ratchet (11), blade (12) and drift (13) are ejected successively,
Connection between cut-out lath micropore, and single micropore is advanced in the hole position on lower section reaction tray (14) (15).Reaction tray
(14) put and can be used to be incubated in isoperibol, and can be rotated according to check problem requirement, so that reaction micropore is sent into machine in Fig. 1
Tool arm (3) position is loaded/fills the middle cleaning of reagent, micropore cleaning unit (4) and optical density reading unit (5) reading light is close
Angle value, is finally discharged to the dustbin below equipment, so as to complete whole measurement process by the complete micropore of colorimetric.
Claims (4)
1. a kind of device for carrying out EUSA, includes microwell plate storage with split cells, storage/defeated in structure
Give/reaction member, liquid reagent storage element, automatic liquid relief unit, micropore cleaning unit, optical density reading unit, its feature
To store detachment device with least one microplate on the device, and using the single micropore that is split into by microplate as
The fixing phase of EUSA, each micropore individually carries out enzyme linked immunosorbent assay.
2. the box and at least of at least one storage lath is included according to the microplate storage detachment device described in claim 1
One lath cropper.
3. according to the cropper described in claim 2, it is characterized in that：In structure ELIAS strip is acted on including following at least one
The part of junction between each micropore, including：Blade, drift, saw blade, heater, laser cutter.
4., according to the single micropore described in claim 1, cut through the cropper described in claim 2 as common microplate
Junction prepares between disconnected micropore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710144044.5A CN106841179A (en) | 2017-03-10 | 2017-03-10 | A kind of EUSA device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710144044.5A CN106841179A (en) | 2017-03-10 | 2017-03-10 | A kind of EUSA device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106841179A true CN106841179A (en) | 2017-06-13 |
Family
ID=59143677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710144044.5A Pending CN106841179A (en) | 2017-03-10 | 2017-03-10 | A kind of EUSA device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106841179A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020134630A1 (en) * | 2018-12-29 | 2020-07-02 | 苏州汶颢微流控技术股份有限公司 | Elisa analyzer |
-
2017
- 2017-03-10 CN CN201710144044.5A patent/CN106841179A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020134630A1 (en) * | 2018-12-29 | 2020-07-02 | 苏州汶颢微流控技术股份有限公司 | Elisa analyzer |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170613 |