CN106834268A - It is a kind of based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria - Google Patents
It is a kind of based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria Download PDFInfo
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- CN106834268A CN106834268A CN201710072175.7A CN201710072175A CN106834268A CN 106834268 A CN106834268 A CN 106834268A CN 201710072175 A CN201710072175 A CN 201710072175A CN 106834268 A CN106834268 A CN 106834268A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
- C02F2101/327—Polyaromatic Hydrocarbons [PAH's]
Abstract
The invention discloses based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria.Diatomite is prepared into substance B through sodium carbonate and sodium dihydrogen phosphate are modified；Through shitosan, N, mixed liquor prepared by N' diformazans oxalamide and expoxy propane is modified to be prepared into material D to substance B；Material D is prepared into material F through mixed liquor prepared by benzoyl amidoxime, 2,4 dihydroxy benzoyl amines, 4,4' dimethyl diphenylamines and acetonitrile is modified；Material F is based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria through the modified material for obtaining of mixed liquor prepared by 3,5 dichloroanilines, 2,6 dimethylanilines, absolute ethyl alcohol, thiocarbamide and tannic acid.
Description
Technical field
It is more particularly to a kind of to be based on silicon the invention belongs to the functional material research and development of environmental pollution treatment technology and preparation field
The functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria of diatomaceous earth.
Background technology
Immobilized microorganism technique is the biological skill that the sixties are grown up by the enzyme immobilization technology in biochemical industry
Art, by means chemically or physically, free cell is positioned at the area of space of restriction, it is kept activity, can be repeatedly
Utilize.In the seventies later stage, increasingly serious with water pollution, the technology is applied to Industry Waste because of its unique advantage
The biological treatment of water, compared with traditional suspended biological facture, immobilized microorganism technique has micro- life in raising reactor
Thing cell concentration, keep high-efficiency strain, microbe density high and be lost in less, reaction speed is fast, resistance to murder by poisoning ability strong, processing equipment
Simple the advantages of, therefore be always in recent years one of focus of domestic and foreign scholars research, while also achieving encouraging grinding
Study carefully achievement.Immobilized microorganism technique can put into practicality it is critical that the selection of fixation support material, is applied to
Microbial immobilized carrier should have that Resistance to microbes, high mechanical strength, mass-transfer performance be good, nontoxic to microorganism, property
The characteristic such as stable, cheap, long lifespan.Currently used fixation support material mainly has：Organic polymer carrier, nothing
Airborne body and complex carrier three major types.Organic polymer carrier is nontoxic to microorganism, mass-transfer performance is good, but low intensity, Yi Beiwei
Biological decomposition, short life.It is for example porous Tao Zhu of inorganic carrier, calcium-base bentonite, kaolin, diatomite, activated carbon, porous silicon, many
Hole glass etc., with mechanical strength it is big, non-toxic to microorganism, be difficult to be decomposed by the microorganisms, mechanical strength is big, it is simple to make,
The characteristics such as acid and alkali-resistance, low cost, long lifespan, application value is larger.But they have, and density is big, realize the efficiency of fluidisation simultaneously
The absorption of high, microorganism is limited and the shortcomings of easily coming off.Therefore need to be modified carrier, so as to improve the property of carrier
Energy.
Polycyclic aromatic hydrocarbon (Polycyclic Aromatic Hydrocarbons, PAHs) be a class contain two or two with
Upper phenyl ring, it is prominent with strong carcinogenesis, teratogenesis and cause with the thick ring-based compounds that wire, horn shape or tufted are arranged
Change is acted on.Due to there is the big π bonding electrons system of conjugation in PAHs molecules, therefore with stability higher, its photolysis and
Biodegradation is slow.Fixed polycyclic aromatic hydrocarbon-degrading bacteria is to realize that recovering PAHs pollutes one of most effective means of environment, but
It is also to lack the functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria at present, therefore, exploitation is based on diatomaceous more for fixation
The functional material of cyclophane surfactant hydrocarbon degradation bacteria, this has great importance for administering and repairing polycyclic aromatic hydrocarbons contaminated environment.
The content of the invention
It is an object of the invention to provide a kind of based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria, its
Preparation method is comprised the following steps that：
(1) to 44.38g diatomite is added in the sodium carbonate liquor that 350mL mass percent concentrations are 12%, it is well mixed
After be filtered to remove liquid and obtain substance A, after substance A is washed through the sodium dihydrogen phosphate that 750mL mass percent concentrations are 3%
Placed 120 minutes in 105 DEG C of drying box, obtain substance B；
(2) 12.64g shitosans are added in 200mL deionized waters, are stirred 15 minutes under the conditions of 1000r/min, obtained
To mixed liquor M；
(3) 3.66 grams of N, N'- diformazans oxalamide and 8mL expoxy propane are added in mixed liquor M, in 1000r/min
Under the conditions of stir 15 minutes, obtain mixed liquor N；
(4) substance B is added in mixed liquor N, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid
Substance C is obtained, substance C is placed in Muffle furnace and is calcined 90 minutes under the conditions of 515 DEG C, obtain material D；
(5) by 8.6 grams of benzoyl amidoximes, 2.3 grams of 2,4- dihydroxy benzoyl amines and 1.8 grams of 4,4'- dimethyl diphenylamines
It is added in 200mL acetonitriles, is stirred 15 minutes under the conditions of 1000r/min, obtains mixed liquor O；
(6) material D is added in mixed liquor O, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid
Material E is obtained, material E is placed in Muffle furnace after being washed through 500mL deionized waters and is calcined 90 minutes under the conditions of 515 DEG C, obtains
Material F；
(7) 12.5 grams of 3,5- dichloroanilines and 14.9 grams of 2,6- dimethylanilines are added in 200mL absolute ethyl alcohols,
Stirred 3 minutes under the conditions of 1000r/min, obtain mixed liquor P；
(8) 5.3 grams of thiocarbamides and 12.8 grams of tannic acid are added in mixed liquor P, 15 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor Q；
(9) material F is added in mixed liquor Q, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid
Material G is obtained, material G is placed in Muffle furnace after being washed through 500mL deionized waters and is calcined 90 minutes under the conditions of 515 DEG C, obtains
Material be based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria.
The beneficial effects of the invention are as follows obtained based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria
The features such as with high mechanical strength, good, stable in properties mass-transfer performance, long lifespan.
Specific embodiment
The invention provides a kind of based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria, below by
One example illustrates to apply process in fact.
Embodiment 1.
(1) to 44.38g diatomite is added in the sodium carbonate liquor that 350mL mass percent concentrations are 12%, it is well mixed
After be filtered to remove liquid and obtain substance A, after substance A is washed through the sodium dihydrogen phosphate that 750mL mass percent concentrations are 3%
Placed 120 minutes in 105 DEG C of drying box, obtain substance B；
(2) 12.64g shitosans are added in 200mL deionized waters, are stirred 15 minutes under the conditions of 1000r/min, obtained
To mixed liquor M；
(3) 3.66 grams of N, N'- diformazans oxalamide and 8mL expoxy propane are added in mixed liquor M, in 1000r/min
Under the conditions of stir 15 minutes, obtain mixed liquor N；
(4) substance B is added in mixed liquor N, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid
Substance C is obtained, substance C is placed in Muffle furnace and is calcined 90 minutes under the conditions of 515 DEG C, obtain material D；
(5) by 8.6 grams of benzoyl amidoximes, 2.3 grams of 2,4- dihydroxy benzoyl amines and 1.8 grams of 4,4'- dimethyl diphenylamines
It is added in 200mL acetonitriles, is stirred 15 minutes under the conditions of 1000r/min, obtains mixed liquor O；
(6) material D is added in mixed liquor O, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid
Material E is obtained, material E is placed in Muffle furnace after being washed through 500mL deionized waters and is calcined 90 minutes under the conditions of 515 DEG C, obtains
Material F；
(7) 12.5 grams of 3,5- dichloroanilines and 14.9 grams of 2,6- dimethylanilines are added in 200mL absolute ethyl alcohols,
Stirred 3 minutes under the conditions of 1000r/min, obtain mixed liquor P；
(8) 5.3 grams of thiocarbamides and 12.8 grams of tannic acid are added in mixed liquor P, 15 points is stirred under the conditions of 1000r/min
Clock, obtains mixed liquor Q；
(9) material F is added in mixed liquor Q, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid
Material G is obtained, material G is placed in Muffle furnace after being washed through 500mL deionized waters and is calcined 90 minutes under the conditions of 515 DEG C, obtains
Material be based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria.
Here be with obtained in the inventive method be based on the diatomaceous function material for fixing polycyclic aromatic hydrocarbon-degrading bacteria
Material has carried out immobilization to polycyclic aromatic hydrocarbon-degrading bacteria, and polycyclic aromatic hydrocarbons contaminated water body is entered with the polycyclic aromatic hydrocarbon-degrading bacteria after fixed
Degradation experiment is gone, has as a result shown：When luxuriant and rich with fragrance initial concentration is 10.9mg/L, the polycyclic aromatic hydrocarbon-degrading bacteria 6 after fixing is added
Luxuriant and rich with fragrance concentration is reduced to 1.1mg/L after it.
Claims (1)
1. it is a kind of based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria, it is characterised in that to prepare the function
Material is comprised the following steps that：
(1) to addition 44.38g diatomite in the sodium carbonate liquor that 350mL mass percent concentrations are 12%, mistake after being well mixed
Filter liquid and obtain substance A, substance A washed through the sodium dihydrogen phosphate that 750mL mass percent concentrations are 3% after
Placed 120 minutes in 105 DEG C of drying box, obtain substance B；
(2) 12.64g shitosans are added in 200mL deionized waters, are stirred 15 minutes under the conditions of 1000r/min, mixed
Close liquid M；
(3) 3.66 grams of N, N'- diformazans oxalamide and 8mL expoxy propane are added in mixed liquor M, in 1000r/min conditions
Lower stirring 15 minutes, obtains mixed liquor N；
(4) substance B is added in mixed liquor N, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid and obtain
Substance C, substance C is placed in Muffle furnace and is calcined 90 minutes under the conditions of 515 DEG C, obtains material D；
(5) 8.6 grams of benzoyl amidoximes, 2.3 grams of 2,4- dihydroxy benzoyl amines and 1.8 grams of 4,4'- dimethyl diphenylamines are added
To in 200mL acetonitriles, stirred 15 minutes under the conditions of 1000r/min, obtain mixed liquor O；
(6) material D is added in mixed liquor O, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid and obtain
Material E, material E are placed in Muffle furnace after being washed through 500mL deionized waters and are calcined 90 minutes under the conditions of 515 DEG C, obtain material
F；
(7) 12.5 grams of 3,5- dichloroanilines and 14.9 grams of 2,6- dimethylanilines are added in 200mL absolute ethyl alcohols,
Stirred 3 minutes under the conditions of 1000r/min, obtain mixed liquor P；
(8) 5.3 grams of thiocarbamides and 12.8 grams of tannic acid are added in mixed liquor P, are stirred 15 minutes under the conditions of 1000r/min,
Obtain mixed liquor Q；
(9) material F is added in mixed liquor Q, is shaken 45 minutes in the shaking table that temperature is 35 DEG C, be filtered to remove liquid and obtain
Material G, material G are placed in Muffle furnace after being washed through 500mL deionized waters and are calcined 90 minutes under the conditions of 515 DEG C, the thing for obtaining
Matter is based on the diatomaceous functional material for fixing polycyclic aromatic hydrocarbon-degrading bacteria.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105624141A (en) * | 2016-02-19 | 2016-06-01 | 曲阜师范大学 | Method for preparing nanometer-magnetic-particle immobilized aniline degrading bacteria and application thereof to degradation of chlorophenol |
| CN107177587A (en) * | 2017-07-30 | 2017-09-19 | 光合强化（北京）生物科技有限公司 | Modification ceramic tile particulate vector for fixing thimet degradation bacteria and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674562A (en) * | 2012-06-04 | 2012-09-19 | 中国科学院生态环境研究中心 | Material and method for repairing polluted water and bottom mud |
| CN104312951A (en) * | 2014-10-13 | 2015-01-28 | 华南理工大学 | Microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as preparation method and application of microbial agent |
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2017
- 2017-02-08 CN CN201710072175.7A patent/CN106834268A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674562A (en) * | 2012-06-04 | 2012-09-19 | 中国科学院生态环境研究中心 | Material and method for repairing polluted water and bottom mud |
| CN104312951A (en) * | 2014-10-13 | 2015-01-28 | 华南理工大学 | Microbial agent capable of degrading polycyclic aromatic hydrocarbons as well as preparation method and application of microbial agent |
Non-Patent Citations (2)
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| 刘洁: "《硅藻土的研究现状及进展》", 《环境科学与管理》 * |
| 鲁光辉: "《硅藻土在废水中的应用及研究现状》", 《中国非金属矿工业导刊》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105624141A (en) * | 2016-02-19 | 2016-06-01 | 曲阜师范大学 | Method for preparing nanometer-magnetic-particle immobilized aniline degrading bacteria and application thereof to degradation of chlorophenol |
| CN107177587A (en) * | 2017-07-30 | 2017-09-19 | 光合强化（北京）生物科技有限公司 | Modification ceramic tile particulate vector for fixing thimet degradation bacteria and preparation method thereof |
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Application publication date: 20170613 |
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